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Cytometry. Part B, Clinical Cytometry

Quentin Baca, Antonio Cosma, Garry Nolan, Brice Gaudilliere
No abstract text is available yet for this article.
November 22, 2016: Cytometry. Part B, Clinical Cytometry
M Lindemann, V Lenz, B Nyadu, F M Heinemann, A Heinold, H Guberina, U Eisenberger, N Lachmann, C Schönemann, A Kribben, A Paul, P A Horn, O Witzke
BACKGROUND: Due to its high sensitivity, the flow cytometry crossmatch (FCXM) has been described as valuable tool for identifying an optimal donor. We here focused on the impact of ABO incompatibility on FCXM results. METHODS: We analyzed 29 ABO incompatible and 89 ABO compatible donor-recipient pairs (73 and 175 data sets, respectively) prior to living donor kidney transplantation. In all patients, lymphocytotoxic crossmatches for B and T cells were negative. RESULTS: Recipients with blood group O (A to O and B to O) displayed significantly (P < 0...
November 18, 2016: Cytometry. Part B, Clinical Cytometry
Wolfgang Kern, Ulrike Bacher, Claudia Haferlach, Tamara Alpermann, Susanne Schnittger, Torsten Haferlach
No abstract text is available yet for this article.
November 14, 2016: Cytometry. Part B, Clinical Cytometry
Jiakui Zhang, Yanjie Ruan, Yuanyuan Shen, Qianshan Tao, Huiping Wang, Lili Tao, Yin Pan, Huizi Fang, Yiping Wang, Zhimin Zhai
Immune thrombocytopenia (ITP) is an autoimmune disorder in which its immune system destroy platelets and lead to haemorrhage symptom. Recent studies have found that regulatory T cells (Tregs) in peripheral blood, bone marrow, and spleen were reduced in ITP patients and recovered after effective ITP therapy. Low-dose Interleukin-2 (IL-2) has been reported recently to increase Tregs and used to treat autoimmune disease including graft-versus-host disease (GVHD) after organ transplantation and HCV-related autoimmune vasculitis...
November 9, 2016: Cytometry. Part B, Clinical Cytometry
Linda Labberton, Andy T Long, Sandra J Gendler, Christine Snozek, Evi X Stavrou, Katrin F Nickel, Coen Maas, Stefan Blankenberg, James S Hernandez, Thomas Renné
BACKGROUND: Platelet polyphosphate is an inorganic procoagulant polymer of orthophosphate units that is stored in dense granules and is released upon platelet activation. Here we describe an assay to measure polyphosphate on the surface of procoagulant human platelets. METHODS AND RESULTS: Recombinant E. coli expressed exopolyphosphatase deletion mutant PPX_Δ12 labeled with fluorescent Alexa488 dye was used as a polyphosphate probe in flow cytometry. PPX_Δ12-Alexa488-signal dose-dependently increased with long-chain polyphosphate binding to platelets...
November 4, 2016: Cytometry. Part B, Clinical Cytometry
Yi Yao, Tobias Welp, Qing Liu, Naiqian Niu, Xiaomei Wang, Clemente J Britto, Smita Krishnaswamy, Geoff L Chupp, Ruth R Montgomery
Airway diseases affect over 7% of the U.S. population and millions of patients worldwide. Asthmatic patients have wide variation in clinical severity with different clinical and physiologic manifestations of disease that may be driven by distinct biologic mechanisms. Further, the immunologic underpinnings of this complex trait disease are heterogeneous and treatment success depends on defining subgroups of asthmatics. Due to the limited availability and number of cells from the lung, the active site, in-depth investigation has been challenging...
November 3, 2016: Cytometry. Part B, Clinical Cytometry
Enikő Balogh, Béla Nagy, Ágnes Gyetvai, Zsolt Bene, Zoltán Hendrik, Viktória Jeney, Péter Nagy, Ágnes Papp, József Balla, György Balla, János Kappelmayer, Béla Nagy
BACKGROUND: Bronchoalveolar mesenchymal stem cells (MSCs) play an important role in the maintenance of lung integrity. Therapeutic application of bone marrow-derived MSCs reduced chronic bronchial inflammation in idiopathic pulmonary fibrosis, and improved the ratio of survivors in sepsis with pneumonia. This study investigated the effect of MSCs from bronchoalveolar lavage fluid (BALF) of hypersensitivity pneumonitis (HP) on T-cell function under in vitro conditions. METHODS: Bronchoalveolar MSCs were obtained via bronchoscopy with BAL from children with severe subacute HP...
October 28, 2016: Cytometry. Part B, Clinical Cytometry
Francis Mandy
No abstract text is available yet for this article.
October 27, 2016: Cytometry. Part B, Clinical Cytometry
B K Singleton, M Ahmed, C A Green, H Heimpel, M J Woźniak, L Ranjha, F Seeney, A Bomford, P Mehta, A Guest, R Mushens, M-J King
BACKGROUND: Bone marrow examination has been the confirmatory test for congenital dyserythropoietic anemia type II (CDAII). Occasional spherocytes on peripheral blood smear can confound the diagnosis. Since a screening test is still unavailable, we explored the feasibility of using flow cytometry as a preliminary screening method. METHODS: Thirteen monoclonal antibodies with specificities for eight erythrocyte membrane proteins were used in FACS analysis to probe the cellular features of red cells from CDAII, normal adults, hereditary spherocytosis (HS), and cord red cells...
October 26, 2016: Cytometry. Part B, Clinical Cytometry
Samaneh Kouzegaran, Soheila Siroosbakht, Bahram Fariborz Farsad, Bijan Rezakhaniha, Banafshe Dormanesh, Vahid Behnod, Amir Saber Tanha
BACKGROUND: In this study, we investigated the role and expression of interleukin (IL)-17A and IL-22 in chronic lymphocytic leukemia Methods: We evaluated the expression of markers above on CLL by ELISA, qRT-PCR, flow cytometric analysis and nonparametric Kruskal-Wallis test. RESULTS: Quantitative RT-PCR revealed that the mRNA levels of IL-17A and IL-22 in PBMCs of CLL patients were upregulated compared with those from healthy subjects (mean ± SD: 1.96 ± 0...
October 8, 2016: Cytometry. Part B, Clinical Cytometry
Dara M Strauss-Albee, Emily C Liang, Thanmayi Ranganath, Natali Aziz, Catherine A Blish
BACKGROUND: Infection is a leading cause of death worldwide in babies under one month of age. Better vaccines and therapeutics are desperately needed for this vulnerable population. METHODS: Because newborns rely heavily on the innate immune system, we evaluated cell phenotype and function of some of the earliest cellular responders during infection, natural killer (NK) cells. We used mass cytometry to provide a comprehensive comparison of NK cells from umbilical cord blood and adult peripheral blood...
October 7, 2016: Cytometry. Part B, Clinical Cytometry
Prashant R Tembhare, Sitaram Ghogale, Nisha Ghatwai, Yajamanam Badrinath, Nikesh Kunder, Nikhil V Patkar, Asma R Bibi, Gaurav Chatterjee, Brijesh Arora, Gaurav Narula, Shripad Banawali, Nilesh Deshpande, Prathibha Amare, Sumeet Gujral, Papagudi G Subramanian
BACKGROUND: Multiparametric flow cytometry (MFC) is a popular technique for minimal residual disease (MRD) analysis. However, its applicability is still limited to 90% of B-cell precursor acute lymphoblastic leukemia (BCPALL) due to two major issues, i.e. a proportion of cases do not express adequate leukemia associated immunophenotype (LAIPs) with currently used markers and drug-induced antigen-modulation. Hence, the incorporation of additional reliable markers is required for the further improvement of MFC-based MRD evaluation...
October 7, 2016: Cytometry. Part B, Clinical Cytometry
Nele Schnipper, Hans H Stassen, Tilmann Kallinich, Karl Sperling, Katrin Hoffmann
The nuclear morphology of neutrophils depends on different endogenous and exogenous factors, which can lead to hypo- or hypersegmentation of the normally 2-4 segmented nucleus. Hyposegmentation can be due to mutations in the LBR-gene (Pelger-Huët-Anomaly) or can be induced, for example, by colchicine treatment. The range of this phenotypic variation is known as "norm of reaction", which can be of major relevance for clinical diagnosis and therapeutic intervention. In this project, we studied the "norm of reaction" in 26 subjects with 0-3 wild type LBR alleles...
September 29, 2016: Cytometry. Part B, Clinical Cytometry
Myung Chul Suh, Jang Soo Suh, Dong Il Won
BACKGROUND: Platelet or neutrophil antibodies (Ab) detected by an indirect method need to be further investigated to confirm that they are HLA (lymphocyte)-specific. This can only be determined by the assessment of lymphocytes from the same donor. We have developed an improved flow cytometric method for the simultaneous detection of Abs against three distinct cell types (platelets, lymphocytes, and neutrophils) in a single suspension. METHODS: We prepared white blood cell (WBC) suspensions using an overlay procedure...
September 15, 2016: Cytometry. Part B, Clinical Cytometry
Lin Hong, Stephanie Chavez, Yelena Smagley, Alexandre Chigaev, Larry A Sklar
BACKGROUND: Cdc42 GTPase has important roles in regulating intracellular actin reorganization. The current methods to monitor actin changes are typically complex and point by point. METHODS: The effects of Cdc42 inhibitors on the side scatter changes were tested in a newly developed continuous assay using the flow cytometer. Staining with fluorescently labeled phalloidin was used for comparison. RESULTS: Cdc42-specific inhibitors caused dose-dependent changes of both the right-angle side scatter and the phalloidin-stained actin...
November 2016: Cytometry. Part B, Clinical Cytometry
Sindhu Cherian, Valerie Miller, Vivian McCullouch, Katy Dougherty, Jonathan R Fromm, Brent L Wood
BACKGROUND: Residual disease detection following therapy is an important prognostic variable in B-lymphoblastic leukemia (B-LL). Most flow cytometric strategies for detecting B cell malignancy utilize CD19 to identify B cells. With growing use of anti-CD19 targeted therapies, alternative strategies are needed for residual disease detection. We describe an approach for residual disease detection in this setting. METHODS: A novel combination was designed using expression of CD22 or CD24 (without CD66b) for B cell detection in combination with markers aberrantly expressed in B-LL (CD10, CD20, CD34, CD38, and CD45)...
September 6, 2016: Cytometry. Part B, Clinical Cytometry
Nalin Leelatian, Deon B Doxie, Allison R Greenplate, Bret C Mobley, Jonathan M Lehman, Justine Sinnaeve, Rondi M Kauffmann, Jay A Werkhaven, Akshitkumar M Mistry, Kyle D Weaver, Reid C Thompson, Pierre P Massion, Mary A Hooks, Mark C Kelley, Lola B Chambless, Rebecca A Ihrie, Jonathan M Irish
BACKGROUND: Mass cytometry measures 36 or more markers per cell and is an appealing platform for comprehensive phenotyping of cells in human tissue and tumor biopsies. While tissue disaggregation and fluorescence cytometry protocols were pioneered decades ago, it is not known whether established protocols will be effective for mass cytometry and maintain cancer and stromal cell diversity. METHODS: Tissue preparation techniques were systematically compared for gliomas and melanomas, patient derived xenografts of small cell lung cancer, and tonsil tissue as a control...
September 6, 2016: Cytometry. Part B, Clinical Cytometry
Marta Morado, Alex Freire Sandes, Enrique Colado, Dolores Subirá, Paloma Isusi, María Soledad Noya, María Belén Vidriales, Amparo Sempere, José Ángel Díaz, Alfredo Minguela, Beatriz Álvarez, Cristina Serrano, Teresa Caballero, Mercedes Rey, Ana Pérez Corral, María Cristina Fernández Jiménez, Elena Magro, Angelina Lemes, Celina Benavente, Helena Bañas, Juana Merino, Celine Castejon, Olivier Gutierrez, Pilar Rabasa, Matheus Vescosi Gonçalves, Martin Perez-Andres, Alberto Orfao
BACKGROUND: Although consensus guidelines have been proposed in 2010 for the diagnostic screening of paroxysmal nocturnal hemoglobinuria (PNH) by flow cytometry (FCM), so far no study has investigated the efficiency of such medical indications in multicentric vs. reference laboratory settings. METHODS: Here we evaluate the efficiency of consensus medical indications for PNH testing in 3,938 peripheral blood samples submitted to FCM testing in 24 laboratories in Spain and one reference center in Brazil...
September 6, 2016: Cytometry. Part B, Clinical Cytometry
J P McCoy
No abstract text is available yet for this article.
September 2016: Cytometry. Part B, Clinical Cytometry
Dharmendra Jain, Pranav Dorwal, Smeeta Gajendra, Amit Pande, Simmi Mehra, Ritesh Sachdev
OBJECTIVE: The aberrant expression of CD5 in both HCL and HCL-v is a very rare event. Although a number of CD5 positive HCL and HCL-v cases have been reported, but these are far and few in between. We aimed to review the reported cases of CD5 positive HCL and its variant. METHOD: We hereby report a case of CD5 positive HCL, with variations in PIK3CA and PDGFRA gene, along with a brief review of literature of the cases of CD5 positive HCL and its variant. RESULTS AND CONCLUSION: The current case was positive for CD103, CD11c, CD25, and CD123 which has led the diagnosis to be of typical HCL...
September 2016: Cytometry. Part B, Clinical Cytometry
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