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Cytometry. Part B, Clinical Cytometry

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https://www.readbyqxmd.com/read/28509416/the-importance-of-cd39-cd43-cd81-and-cd95-expression-for-differentiating-b-cell-lymphoma-by-flow-cytometry
#1
Chandra Chiappin Cardoso, Mariangeles Auat, Iris Mattos Santos-Pirath, Renata Cristina Messores Rudolf-Oliveira, Jessica Pires da Silva, Bárbara Gil Lange, Danielle Siegel, Ana Carolina Rabello de Moraes, Joanita Angela Gonzaga Del Moral, Maria Claudia Santos-Silva
BACKGROUND: B cell lymphomas' (BCL) current diagnosis is usually based on a combination of morphology, immunophenotype, recurrent cytogenetic aberration and clinical features. However, even with these diagnostic tools, a definitive diagnosis can be difficult to achieve. Therefore, the aim of this study was to assess the profile of CD39, CD43, CD81, and CD95 expressions in diffuse large B cell lymphoma (DLBCL), follicular lymphoma (FL), and Burkitt lymphoma (BL) cases. METHODS: To address this issue, we investigated the expression of CD39, CD43, CD81, and CD95 by eight-color flow cytometry in retrospective cases from 2014 to 2016...
May 16, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28493330/improved-panels-for-clinical-immune-phenotyping-utilization-of-the-violet-laser
#2
Mark Ryherd, Matthew Plassmeyer, Connor Alexander, Ines Eugenio, Yuliya Kleschenko, Ariel Badger, Raavi Gupta, Oral Alpan, Søren Ulrik Sønder
BACKGROUND: Clinical diagnostic laboratories are subject to numerous regulations imposed by government agencies. Laboratory developed tests for flow cytometry panels are essentially restricted to the use of analyte-specific reagents (ASR) antibodies. With the advances in clinical flow cytometry systems, there is a trend toward the utilization of blue/red/violet laser flow systems and 8 to 10-color panels. Currently, the selection of commercially available ASR antibodies for the violet laser is very limited...
May 10, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28480599/laboratory-accuracy-improvement-in-the-uk-neqas-leucocyte-immunophenotyping-immune-monitoring-programme-an-eleven-year-review-via-longitudinal-mixed-effects-modeling
#3
John Bainbridge, Wes Rountree, Raul Louzao, John Wong, Liam Whitby, Thomas N Denny, David Barnett
BACKGROUND: The United Kingdom National External Quality Assessment Service (UK NEQAS) for Leucocyte Immunophenotyping Immune Monitoring Programme, provides external quality assessment (EQA) to non-U.S. laboratories affiliated with the NIH NIAID Division of AIDS (DAIDS) clinical trials networks. Selected laboratories are required to have oversight, performance monitoring and remediation undertaken by Immunology Quality Assessment (IQA) staff under the DAIDS contract. We examined whether laboratory accuracy improves with longer EQA participation and whether IQA remediation is effective...
May 8, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28475275/a-qa-program-for-mrd-testing-demonstrates-that-systematic-education-can-reduce-discordance-among-experienced-interpreters
#4
Michael Keeney, Brent L Wood, Benjamin D Hedley, Joseph A DiGiuseppe, Maryalice Stetler-Stevenson, Elisabeth Paietta, Gerard Lozanski, Adam C Seegmiller, Bruce W Greig, Aaron C Shaver, Lata Mukundan, Howard R Higley, Caroline C Sigman, Gary Kelloff, J Milburn Jessup, Michael J Borowitz
BACKGROUND: Minimal residual disease (MRD) in B lymphoblastic leukemia (B-ALL) by flow cytometry is an established prognostic factor used to adjust treatment in most pediatric therapeutic protocols. MRD in B-ALL has been standardized by the Children's Oncology Group (COG) in North America, but not routine clinical labs. The Foundation for National Institutes of Health sought to harmonize MRD measurement among COG, oncology groups, academic, community and government, laboratories. METHODS: Listmode data from post-induction marrows were distributed from a reference lab to seven different clinical FCM labs with variable experience in B-ALL MRD...
May 5, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28449204/an-escca-initiative-on-advanced-analyses-of-monocyte-lineage-using-flow-cytometry
#5
Claude Lambert, Ulrich Sack
No abstract text is available yet for this article.
April 27, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28431200/the-utility-of-flow-cytometry-in-differentiating-nk-t-cell-lymphoma-from-indolent-and-reactive-nk-cell-proliferations
#6
Sanjay de Mel, Jenny Bei Li, Muhammad Bilal Abid, Tiffany Pooi Ling Tang, Hui Ming Tay, Wen Chang Ting, Li Mei Poon, Tae Hoon Chung, Benjamin Mow, Allison Tso, Kiat Hoe Ong, Wee Joo Chng, Te Chih Liu
BACKGROUND: The WHO defines three categories of NK cell malignancies; extra nodal NK/T cell lymphoma (NKTCL), aggressive NK cell leukemia, and the provisional entity chronic lymphoproliferative disorder of NK cells (CLPD-NK). Although the flow cytometric (FC) phenotype of CLPD-NK has been described, studies on FC phenotype of NKTCL are limited. To the best of our knowledge ours is the first study to compare the phenotype of NKTCL, CLPD-NK, reactive NK lymphocytosis (RNKL), and normal NK cells using eight color (8C) FC...
April 21, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28378896/a-comprehensive-assessment-of-immunophenotyping-performed-in-cryopreserved-peripheral-whole-blood
#7
Chris P Verschoor, Vikas Kohli, Cynthia Balion
BACKGROUND: Monitoring the frequency and phenotype of white blood cell subsets using flow cytometry (immunophenotyping) has proven to be an incredibly powerful tool in the assessment of health. Although improved technologies have aided in the practical implementation of immunophenotyping in clinical and epidemiological studies, the transportation of blood from the site of collection to a central laboratory for analysis within a reasonable timeframe may not be feasible. Hence, the purpose of the following study was to investigate the validity of cryopreserved whole blood as a simple to prepare and cost-effective biospecimen for multi-colour immunophenotyping in a large epidemiological study-namely, The Canadian Longitudinal Study on Aging (CLSA)...
April 5, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28378895/monitoring-cd49d-receptor-occupancy-a-method-to-optimize-and-personalize-natalizumab-therapy-in-multiple-sclerosis-patients
#8
Joan Puñet-Ortiz, José Vicente Hervás-García, Aina Teniente-Serra, Antonio Cano-Orgaz, Maria José Mansilla, Bibiana Quirant-Sánchez, Juan Navarro-Barriuso, Marco A Fernández-Sanmartín, Silvia Presas-Rodríguez, Cristina Ramo-Tello, Eva María Martínez-Cáceres
BACKGROUND: In natalizumab-treated relapsing-remitting MS (RRMS) patients, various extended interval dosing strategies are under evaluation to minimize severe treatment-associated side effects, mainly progressive multifocal leukoencephalopathy development. Up to now, it has not been presented any approach, even in form of assay design, to determine the optimal percentage of CD49d receptor occupancy (RO) associated with a favorable clinical, radiological, and immunological response. METHODS: A multiparametric quantitative flow cytometry method was settled to measure CD49d RO on peripheral blood lymphocytes...
April 5, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28321976/a-5-color-flow-cytometric-method-for-extended-8-part-leukocyte-differential
#9
Julien Guy, Orianne Wagner-Ballon, Olivier Pages, François Bailly, Jessica Borgeot, Marie-C Béné, Marc Maynadié
OBJECTIVES: Microscopic leukocyte differentials display many drawbacks. Several single 5 to 8-color tubes using multiparameter flow cytometry (MFC) are able to provide extended differentials with sequential gating-based analysis strategies. We investigated a new 5-color MFC method to perform an extended 8-part differential with a simplified gating strategy. METHODS: Whole blood was stained with a combination of antibodies including HLA-DR-FITC/CD19-PE/CD45-ECD/CD16-PC5+CD71-PC5/CD5-PC7...
March 21, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28316130/simultaneous-use-of-multiplex-ligation-dependent-probe-amplification-assay-and-flow-cytometric-dna-ploidy-analysis-in-patients-with-acute-leukemia
#10
Virginia Reyes-Núñez, Evelyn Galo-Hooker, Beatriz Pérez-Romano, Ricardo E Duque, Alejandro Ruiz-Arguelles, Javier Garcés-Eisele
BACKGROUND: The aim of this work was to simultaneously use multiplex ligation-dependent probe amplification (MLPA) assay and flow cytometric DNA ploidy analysis (FPA) to detect aneuploidy in patients with newly diagnosed acute leukemia. METHODS: MLPA assay and propidium iodide FPA were used to test samples from 53 consecutive patients with newly diagnosed acute leukemia referred to our laboratory for immunophenotyping. Results were compared by nonparametric statistics...
March 18, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28296063/choosing-a-new-cd4-technology-can-statistical-method-comparison-tools-influence-the-decision
#11
Lesley E Scott, Luc Kestens, Kovit Pattanapanyasat, Kasma Sukapirom, Wendy S Stevens
BACKGROUND: Method comparison tools are used to determine the accuracy, precision, agreement, and clinical relevance of a new or improved technology versus a reference technology. Guidelines for the most appropriate method comparison tools as well as their acceptable limits are lacking and not standardized for CD4 counting technologies. METHODS: Different method comparison tools were applied to a previously published CD4 dataset (n = 150 data pairs) evaluating five different CD4 counting technologies (TruCOUNT, Dual Platform, FACSCount, Easy CD4, CyFlow) on a single specimen...
March 10, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28257592/visualization-of-cell-composition-and-maturation-in-the-bone-marrow-using-10-color-flow-cytometry-and-radar-plots
#12
Katayoon Jafari, Anne Tierens, Amr Rajab, Rumina Musani, André Schuh, Anna Porwit
BACKGROUND: The enormous potential of complex data files generated by 10-color flow cytometry (FC) is hindered by the requirement for exhaustive manual gating and the complexity of multidimensional data visualization. We propose a model using radar plots (RPs), to improve FC data visualization by capturing multidimensionality and integration of FC findings. METHOD: We analysed 12 normal/reactive bone marrow (N/R BM) samples and 12 BM samples from patients with myelodysplasia (MDS) with 10-color FC...
March 3, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28504481/comprehensive-mass-cytometry-analysis-of-cell-cycle-activation-and-co-inhibitory-receptors-expression-in-cd4-t-cells-from-healthy-and-hiv-infected-individuals
#13
(no author information available yet)
No abstract text is available yet for this article.
May 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28266139/-markers-and-function-of-human-nk-cells-in-normal-and-pathological-conditions
#14
EDITORIAL
Andreas Lundqvist, Veronika Kremer
No abstract text is available yet for this article.
March 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28054442/markers-and-function-of-human-nk-cells-in-normal-and-pathological-conditions
#15
REVIEW
Genny Del Zotto, Emanuela Marcenaro, Paola Vacca, Simona Sivori, Daniela Pende, Mariella Della Chiesa, Francesca Moretta, Tiziano Ingegnere, Maria Cristina Mingari, Alessandro Moretta, Lorenzo Moretta
Natural killer (NK) cells, the most important effectors of the innate lymphoid cells (ILCs), play a fundamental role in tumor immune-surveillance, defense against viruses and, in general, in innate immune responses. NK cell activation is mediated by several activating receptors and co-receptors able to recognize ligands on virus-infected or tumor cells. To prevent healthy cells from auto-aggression, NK cells are provided with strong inhibitory receptors (KIRs and NKG2A) which recognize HLA class I molecules on target cells and, sensing their level of expression, allow killing of targets underexpressing HLA-class I...
March 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/27416483/polychromatic-flow-cytometry-is-more-sensitive-than-microscopy-in-detecting-small-monoclonal-plasma-cell-populations
#16
Daniel N Tran, Sandy A B C Smith, David A Brown, Andrew J C Parker, Joanne E Joseph, Nicola Armstrong, William A Sewell
BACKGROUND: There is an emerging role for flow cytometry (FC) in the assessment of small populations of plasma cells (PC). However, FC's utility has been questioned due to consistent underestimation of the percentage of PC compared to microscopy. METHODS: A retrospective study was performed on bone marrow samples analysed by 8-colour FC. Plasma cell populations were classified as polyclonal or monoclonal based on FC analysis. FC findings were compared with microscopy of aspirates, histology and immunohistochemistry of trephine biopsies, and immunofixation (IFX) of serum and/or urine...
March 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/27282966/translating-microfluidics-cell-separation-technologies-and-their-barriers-to-commercialization
#17
REVIEW
C Wyatt Shields, Korine A Ohiri, Luisa M Szott, Gabriel P López
Advances in microfluidic cell sorting have revolutionized the ways in which cell-containing fluids are processed, now providing performances comparable to, or exceeding, traditional systems, but in a vastly miniaturized format. These technologies exploit a wide variety of physical phenomena to manipulate cells and fluid flow, such as magnetic traps, sound waves and flow-altering micropatterns, and they can evaluate single cells by immobilizing them onto surfaces for chemotherapeutic assessment, encapsulate cells into picoliter droplets for toxicity screenings and examine the interactions between pairs of cells in response to new, experimental drugs...
March 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/27001939/expression-of-death-receptor-3-on-peripheral-blood-mononuclear-cells-differes-in-adult-ibd-patients-and-children-with-newly-diagnosed-ibd
#18
Tomasz J Slebioda, Agnieszka Bojarska-Junak, Marta Cyman, Piotr Landowski, Barbara Kaminska, Krzysztof Celinski, Zbigniew Kmiec
BACKGROUND: Interaction between TL1A and death receptor 3 (DR3) is associated with the pathogenesis of inflammatory bowel disease (IBD), although their role in the development of this disease remains not fully explained. Some studies showed elevated expression of TL1A and DR3 in inflamed intestinal tissue but currently there are no reports concerning expression of DR3 on peripheral blood mononuclear cells (PBMCs) of IBD patients which was the subject of our study. METHODS: We performed flow cytometry analysis of DR3 expression on CD4(+), CD8(+), CD11c(+), CD14(+) or CD20(+) PBMCs of adults and children with IBD and healthy volunteers with respect to C-reactive protein (CRP) levels in blood...
March 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/26918565/streptamer-technology-allows-accurate-and-specific-detection-of-cmv-specific-hla-a-02-cd8-t-cells-by-flow-cytometry
#19
Miriam Ciáurriz, Lorea Beloki, Eva Bandrés, Cristina Mansilla, Amaya Zabalza, Estela Pérez-Valderrama, Mercedes Lachén, Berta Ibáñez, Eduardo Olavarría, Natalia Ramírez
BACKGROUND: Multimer technology is widely used to screen antigen-specific immune recovery after allogeneic hematopoietic stem cell transplantation (allo-HSCT) as it enables identification, enumeration, phenotypic characterization and isolation of virus-specific T-cells. Novel approaches of multimerization might improve on classical tetramer staining; however, their use as standard monitoring technique to quantify antigen-specific cells has not been validated yet. We have compared two of these available multimeric complexes: pentamer and streptamer to select the best strategy for the incorporation into clinical monitoring practice...
March 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/26666746/potential-therapeutic-targets-in-plasma-cell-disorders-a-flow-cytometry-study
#20
Katharina Lisenko, Stefan Schönland, Ute Hegenbart, Katrin Wallenwein, Ute Braun, Elias K Mai, Jens Hillengass, Hartmut Goldschmidt, Anna Jauch, Anthony D Ho, Marc Raab, Michael Hundemer
The discovery of new targets for tailored therapy is a major improvement in oncology, and tools for the rapid and reliable detection of these targets are essential. Clinical trials demonstrated the benefit of recently developed antibodies against antigens on malignant B-cells. The aim of this study was to assess patients with plasma cell (PC) disorders for expression of antigens on malignant PCs that have exhibited promise in targeted cancer therapy. We retrospectively analyzed the expression of CD20, CD22, CD27, CD30, CD38, CD52, CD81, CD138, and SLAMF7 on PCs by flow cytometry in 103 patients with PC disorders...
March 2017: Cytometry. Part B, Clinical Cytometry
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