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Cytometry. Part B, Clinical Cytometry

Katharina Kriegsmann, Tobias Dittrich, Brigitte Neuber, Mohamed H S Awwad, Ute Hegenbart, Hartmut Goldschmidt, Jens Hillengass, Dirk Hose, Anja Seckinger, Carsten Müller-Tidow, Anthony D Ho, Stefan Schönland, Michael Hundemer
BACKGROUND: Recent approaches in multiple myeloma (MM) treatment have targeted CD38. As antigen expression levels on plasma cells (PCs) were demonstrated to affect response to monoclonal antibody (mAb) treatment, a precise characterization of PC phenotype is warranted. METHODS: Anti-CD38 mAb (isatuximab) was tested for antibody-dependent cellular cytotoxicity (ADCC) in MM cell lines. Quantification of the number of sites (NOS) of CD38 on bone marrow PCs and other immune cells obtained from light chain (AL) amyloidosis (n=46) and smoldering multiple myeloma (SMM) patients (n=19) was performed with two different quantitative flow cytometry (QFCM) applications...
March 25, 2018: Cytometry. Part B, Clinical Cytometry
Steven R Post, Ginell R Post, Dejan Nikolic, Rebecca Owens, Giovanni Insuasti-Beltran
Background - Despite increased usage of multiparameter flow cytometry (MFC) to assess diagnosis, prognosis, and therapeutic efficacy (minimal residual disease - MRD) in plasma cell neoplasms (PCNs), standardization of methodology and data analysis is suboptimal. We investigated the utility of using the mean and median fluorescence intensities (FI) obtained from MFC to objectively describe parameters that distinguish plasma cell (PC) phenotypes. Methods - In this retrospective study, flow cytometry results from bone marrow aspirate specimens from 570 patients referred to the Myeloma Institute at UAMS were evaluated...
March 24, 2018: Cytometry. Part B, Clinical Cytometry
Pei-Dong Chi, Natalie S Freed, Laura Wake, Cynthia Page, Lynette Smith, Catalina Amador, Hina Naushad Qureishi, Timothy Greiner, Scott Rodig, Samuel Pirruccello, Ji Yuan, Kai Fu
OBJECTIVES: We developed a simple and effective rinsing technique of needle biopsies to produce cell suspensions for flow cytometry and evaluated if the rinsing technique is comparable to the conventional tissue cell suspension technique. METHODS: We retrieved 93 needle core biopsy cases employing the rinsing technique (RT) for flow cytometry and 25 needle biopsy cases using tissue cell suspension (TCS) for flow cytometry. RESULTS: The diagnostic concordance between the flow cytometry results and the morphologic diagnoses of both groups was compared...
March 13, 2018: Cytometry. Part B, Clinical Cytometry
Alessandro Moretta
No abstract text is available yet for this article.
February 28, 2018: Cytometry. Part B, Clinical Cytometry
Boonyanuch Chutvanichkul, Phantip Vattanaviboon, Sumana Mas-Oodi, Yaowalak U-Pratya, Wanchai Wanachiwanawin
BACKGROUND: Labile iron pool (LIP) is intracellular non-protein bound iron that can generate oxygen radicals via the Fenton reaction resulting in oxidative cell damage. Therefore, quantitative measurement of LIP will be helpful for detecting and monitoring the toxic iron status in iron overloaded patients. This study demonstrated LIP level and its correlation to oxidative stress status in β-thalassemic erythrocytes. METHODS: LIP and reactive oxygen species (ROS) level, numbers of erythrocyte vesicles and apoptosis were assayed by flow cytometric methods in 30 blood samples from β-thalassemia/hemoglobin E patients and 17 blood samples from healthy volunteers with normal hemoglobin type...
February 27, 2018: Cytometry. Part B, Clinical Cytometry
Yang Zeng, Alan Hiti, Sherry Moranville, Gloria Vicent, Sylvia Chavira, Monika de Arruda Indig, Sharon Graminske, Amanda Boerner, Anna Schmidt, Farzad Oreizy, Angela Chen, Maryam Saleminik, Fred Mosqueda, Anna Lin, Kevin Judge
The BD FACSVia™ system* is a novel flow cytometer with improved workflow efficiencies. To evaluate the HLA-B27 application developed on the BD FACSVia system utilizing the BD™ HLA-B27 kit, we conducted a concordance study at three centers to compare with the BD FACSCalibur™ system. Prepared donor samples (n = 594) were analyzed on both the BD FACSVia and BD FACSCalibur for the HLA-B27 assay. Adjudication of HLA-B27 discordant results was performed using the Reverse Sequence Specific Oligonucleotide (rSSO) DNA typing method (LABType® SSO, One Lambda)...
February 24, 2018: Cytometry. Part B, Clinical Cytometry
Mariangeles Auat, Chandra Chiappin Cardoso, Iris Mattos Santos-Pirath, Renata Cristina Messores Rudolf-Oliveira, Camila Matiollo, Bárbara Gil Lange, Jessica Pires da Silva, Gisele Cristina Dametto, Mayara Marin Pirolli, Maria Daniela Holthausen Perico Colombo, Maria Claudia Santos-Silva
BACKGROUND: Flow cytometric immunophenotyping is deemed a fundamental tool for the diagnosis of B-cell neoplasms. Currently, the investigation of novel immunophenotypic markers has gained importance, as they can assist in the precise subclassification of B-cell malignancies by flow cytometry. Therefore, the purpose of the present study was to evaluate the expression of CD307a during normal B-cell maturation and in B-cell malignancies as well as to investigate its potential role in the differential diagnosis of these entities...
February 24, 2018: Cytometry. Part B, Clinical Cytometry
Bruno Brando
No abstract text is available yet for this article.
February 13, 2018: Cytometry. Part B, Clinical Cytometry
Adam Viktorisson, Sherin T Mathew, Ola Hammarsten, Pegah Johansson
BACKGROUND: The phosphorylation of histone H2AX (γ-H2AX) at the DNA double-strand break (DSB) site is frequently used for quantifying DSBs and may be useful as a biomarker for clinical applications. We have previously reported a flow cytometry-based quantification of γ-H2AX for clinical routine. One major challenge, however, is the lack of a control sample for normalization of the day-to-day variation of the flow cytometry γ-H2AX assay. METHODS: Here, we report development of a mix-control sample containing peripheral blood mononuclear cells (PBMC) from 10 control individuals, for normalization of day-to-day variation of the flow cytometry-γ-H2AX assay...
February 7, 2018: Cytometry. Part B, Clinical Cytometry
D Robert Sutherland, Fernando Ortiz, Graeme Quest, Andrea Illingworth, Miroslav Benko, Rakesh Nayyar, Iuri Marinov
BACKGROUND: Paroxysmal Nocturnal Hemoglobinuria (PNH) is a rare acquired hematopoietic stem cell disorder characterized by an inability to make Glyco-Phosphatidyl-Inositol (GPI)-linked cell surface structures. Fluorescent proaerolysin (FLAER-Alexa488) is increasingly used to detect GPI-deficient WBCs by flow cytometry. However, FLAER is not available in all countries and is expensive to obtain in others. An earlier study to compare FLAER-based and non-FLAER assays confirmed very good agreement between the two tubes suggesting a cost effective simultaneous evaluation of PNH neutrophils and monocytes is possible without FLAER...
January 30, 2018: Cytometry. Part B, Clinical Cytometry
Johanna Blaha, Klaus Schwarz, Claudia Fischer, Peter Schauwecker, Britta Höchsmann, Hubert Schrezenmeier, Markus Anliker
BACKGROUND: CD157, encoded by BST-1, has been described as a useful flow cytometric marker for the analysis of paroxysmal nocturnal hemoglobinuria (PNH) as it is a glycosylphosphatidylinositol (GPI)-linked molecule highly expressed on normal monocytes and neutrophils. We and others observed isolated CD157 signal dropouts during intended PNH analysis. We hypothesize that these negative populations occur due to an antibody failure. To investigate the reason for this finding, we compared two different anti-CD157 antibody clones for PNH analysis...
January 23, 2018: Cytometry. Part B, Clinical Cytometry
Sang Hyuk Park, Chan-Jeoung Park, Borae G Park, Mi-Hyun Bae, Bo-Hyun Kim, Young-Uk Cho, Seongsoo Jang, Ae-Ja Park, Dae-Young Kim, Jung-Hee Lee, Je-Hwan Lee, Kyoo-Hyung Lee
BACKGROUND: We prospectively evaluated prognostic value of lymphocyte subpopulations in peripheral blood of allogeneic hematopoietic stem cell transplant (HSCT) recipients. METHODS: 113 allogeneic HSCT (47 sibling matched, 37 unrelated matched, 29 haploidentical)-performed patients diagnosed as AML (n = 66), ALL (n = 28), and MDS (n = 19) were prospectively enrolled. 14 lymphocyte subpopulations were quantified by flow cytometry of PB at specific time-points after HSCT, and their prognostic impacts were analyzed...
March 2018: Cytometry. Part B, Clinical Cytometry
Prashant R Tembhare, Sitaram Ghogale, Wilma Tauro, Yajamanam Badrinath, Nilesh Deshpande, Shweta Kedia, Keziah Cherian, Nikhil V Patkar, Gaurav Chatterjee, Sumeet Gujral, Papagudi G Subramanian
BACKGROUND: Current flow-cytometric plasma cell (PC) gating is based on CD138, CD38, and CD45 expression. CD138 is known for variable expression and loss during storage and processing. Introduction of anti-CD38 and anti-CD138 monoclonal-antibody therapies has limited the use of these markers during follow-up. Hence, additional reliable PC-gating markers are required. Recently, CD229 has been claimed as an alternative PC-gating marker. However, these studies are limited to a small cohort of samples...
January 9, 2018: Cytometry. Part B, Clinical Cytometry
Fabien Pitoiset, Michèle Barbié, Guillaume Monneret, Cécile Braudeau, Pierre Pochard, Isabelle Pellegrin, Jacques Trauet, Myriam Labalette, David Klatzmann, Michelle Rosenzwajg
BACKGROUND: Quantification of regulatory T cells (Tregs) is crucial in immunomonitoring in clinical trials as this cell population has been shown to be involved in a wide range of diseases, including cancers, autoimmune diseases, infections, and allergies. Human Tregs are defined as CD4+ CD25+ CD127low FoxP3+ cells, and the standardization of their staining by flow cytometry is a challenge, especially in multicenter clinical trials, notably because of the intracellular location of FoxP3...
January 6, 2018: Cytometry. Part B, Clinical Cytometry
Sarika Gupta, Nitin J Karandikar, Timothy Ginader, Andrew M Bellizzi, Carol J Holman
BACKGROUND: Multiparametric flow cytometry is a useful tool for diagnosis of plasma cell (PC) dyscrasias and assessment of minimal residual disease in plasma cell myeloma (PCM). However, the immunophenotypic differences between the clonal PCs of PCM and those of monoclonal gammopathy of undetermined significance (MGUS) as well as the correlation of these flow cytometric markers with pertinent laboratory parameters have not been evaluated. METHODS: We retrospectively identified all newly diagnosed treatment-naive PCM and MGUS patients between 09/2014 and 06/2015 who underwent 10-color flow-cytometric evaluation: CD45, CD38, CD138, cKappa, cLambda, CD19, CD27, CD28, CD56, CD117...
January 6, 2018: Cytometry. Part B, Clinical Cytometry
Marc Sorigue, Mireia Franch-Sarto, Edurne Sarrate, Jordi Junca
BACKGROUND: The CLLflow score was recently suggested as an improvement over the Moreau score (MS) for the diagnosis and classification of B-cell lymphoproliferative disorders (B-LPD). METHODS: We determined the CLLflow score in peripheral blood or bone marrow of a series of cases with an inconclusive immunophenotype, including samples with a MS of 3 (n = 52) and CD5-positive with a score of 2 (n = 38). As controls, B-LPD with a MS of 0-1 (n = 95), CD5-negative score 2 (n = 24), and score 4-5 (i...
January 6, 2018: Cytometry. Part B, Clinical Cytometry
Giovanni Carulli, Francesco Caracciolo, Paola Sammuri, Cristiana Domenichini, Angela Tarasco, Martina Rousseau, Virginia Ottaviano, Mario Petrini
No abstract text is available yet for this article.
January 2, 2018: Cytometry. Part B, Clinical Cytometry
Stephen J Richards
No abstract text is available yet for this article.
January 2018: Cytometry. Part B, Clinical Cytometry
F Preffer
No abstract text is available yet for this article.
January 2018: Cytometry. Part B, Clinical Cytometry
F Preffer
No abstract text is available yet for this article.
January 2018: Cytometry. Part B, Clinical Cytometry
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