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Cytometry. Part B, Clinical Cytometry

Samaneh Kouzegaran, Soheila Siroosbakht, Bahram Fariborz Farsad, Bijan Rezakhaniha, Banafshe Dormanesh, Vahid Behnod, Amir Saber Tanha
BACKGROUND: In this study, we investigated the role and expression of interleukin (IL)-17A and IL-22 in chronic lymphocytic leukemia Methods: We evaluated the expression of markers above on CLL by ELISA, qRT-PCR, flow cytometric analysis and nonparametric Kruskal-Wallis test. RESULTS: Quantitative RT-PCR revealed that the mRNA levels of IL-17A and IL-22 in PBMCs of CLL patients were upregulated compared with those from healthy subjects (mean ± SD: 1.96 ± 0...
October 8, 2016: Cytometry. Part B, Clinical Cytometry
Dara M Strauss-Albee, Emily C Liang, Thanmayi Ranganath, Natali Aziz, Catherine A Blish
BACKGROUND: Infection is a leading cause of death worldwide in babies under one month of age. Better vaccines and therapeutics are desperately needed for this vulnerable population. METHODS: Because newborns rely heavily on the innate immune system, we evaluated cell phenotype and function of some of the earliest cellular responders during infection, natural killer (NK) cells. We used mass cytometry to provide a comprehensive comparison of NK cells from umbilical cord blood and adult peripheral blood...
October 7, 2016: Cytometry. Part B, Clinical Cytometry
Prashant R Tembhare, Sitaram Ghogale, Nisha Ghatwai, Yajamanam Badrinath, Nikesh Kunder, Nikhil V Patkar, Asma R Bibi, Gaurav Chatterjee, Brijesh Arora, Gaurav Narula, Shripad Banawali, Nilesh Deshpande, Prathibha Amare, Sumeet Gujral, Papagudi G Subramanian
BACKGROUND: Multiparametric flow cytometry (MFC) is a popular technique for minimal residual disease (MRD) analysis. However, its applicability is still limited to 90% of B-cell precursor acute lymphoblastic leukemia (BCPALL) due to two major issues, i.e. a proportion of cases do not express adequate leukemia associated immunophenotype (LAIPs) with currently used markers and drug-induced antigen-modulation. Hence, the incorporation of additional reliable markers is required for the further improvement of MFC-based MRD evaluation...
October 7, 2016: Cytometry. Part B, Clinical Cytometry
Nele Schnipper, Hans H Stassen, Tilmann Kallinich, Karl Sperling, Katrin Hoffmann
The nuclear morphology of neutrophils depends on different endogenous and exogenous factors, which can lead to hypo- or hypersegmentation of the normally 2-4 segmented nucleus. Hyposegmentation can be due to mutations in the LBR-gene (Pelger-Huët-Anomaly) or can be induced, for example, by colchicine treatment. The range of this phenotypic variation is known as "norm of reaction", which can be of major relevance for clinical diagnosis and therapeutic intervention. In this project, we studied the "norm of reaction" in 26 subjects with 0-3 wild type LBR alleles...
September 29, 2016: Cytometry. Part B, Clinical Cytometry
Myung Chul Suh, Jang Soo Suh, Dong Il Won
BACKGROUND: Platelet or neutrophil antibodies (Ab) detected by an indirect method need to be further investigated to confirm that they are HLA (lymphocyte)-specific. This can only be determined by the assessment of lymphocytes from the same donor. We have developed an improved flow cytometric method for the simultaneous detection of Abs against three distinct cell types (platelets, lymphocytes, and neutrophils) in a single suspension. METHODS: We prepared white blood cell (WBC) suspensions using an overlay procedure...
September 15, 2016: Cytometry. Part B, Clinical Cytometry
Sindhu Cherian, Valerie Miller, Vivian McCullouch, Katy Dougherty, Jonathan R Fromm, Brent L Wood
BACKGROUND: Residual disease detection following therapy is an important prognostic variable in B-lymphoblastic leukemia (B-LL). Most flow cytometric strategies for detecting B cell malignancy utilize CD19 to identify B cells. With growing use of anti-CD19 targeted therapies, alternative strategies are needed for residual disease detection. We describe an approach for residual disease detection in this setting. METHODS: A novel combination was designed using expression of CD22 or CD24 (without CD66b) for B cell detection in combination with markers aberrantly expressed in B-LL (CD10, CD20, CD34, CD38, and CD45)...
September 6, 2016: Cytometry. Part B, Clinical Cytometry
Nalin Leelatian, Deon B Doxie, Allison R Greenplate, Bret C Mobley, Jonathan M Lehman, Justine Sinnaeve, Rondi M Kauffmann, Jay A Werkhaven, Akshitkumar M Mistry, Kyle D Weaver, Reid C Thompson, Pierre P Massion, Mary A Hooks, Mark C Kelley, Lola B Chambless, Rebecca A Ihrie, Jonathan M Irish
BACKGROUND: Mass cytometry measures 36 or more markers per cell and is an appealing platform for comprehensive phenotyping of cells in human tissue and tumor biopsies. While tissue disaggregation and fluorescence cytometry protocols were pioneered decades ago, it is not known whether established protocols will be effective for mass cytometry and maintain cancer and stromal cell diversity. METHODS: Tissue preparation techniques were systematically compared for gliomas and melanomas, patient derived xenografts of small cell lung cancer, and tonsil tissue as a control...
September 6, 2016: Cytometry. Part B, Clinical Cytometry
Marta Morado, Alex Freire Sandes, Enrique Colado, Dolores Subirá, Paloma Isusi, María Soledad Noya, María Belén Vidriales, Amparo Sempere, José Ángel Díaz, Alfredo Minguela, Beatriz Álvarez, Cristina Serrano, Teresa Caballero, Mercedes Rey, Ana Pérez Corral, María Cristina Fernández Jiménez, Elena Magro, Angelina Lemes, Celina Benavente, Helena Bañas, Juana Merino, Celine Castejon, Olivier Gutierrez, Pilar Rabasa, Matheus Vescosi Gonçalves, Martin Perez-Andres, Alberto Orfao
BACKGROUND: Although consensus guidelines have been proposed in 2010 for the diagnostic screening of paroxysmal nocturnal hemoglobinuria (PNH) by flow cytometry (FCM), so far no study has investigated the efficiency of such medical indications in multicentric vs. reference laboratory settings. METHODS: Here we evaluate the efficiency of consensus medical indications for PNH testing in 3,938 peripheral blood samples submitted to FCM testing in 24 laboratories in Spain and one reference center in Brazil...
September 6, 2016: Cytometry. Part B, Clinical Cytometry
A L Henning, A S Venable, J L Vingren, D W Hill, B K McFarlin
BACKGROUND: Macrophage-derived foam cells are the predominant component of arterial plaques in the early stages of atherosclerosis. One factor that poses a major risk for plaque development is high levels of plasma low-density lipoprotein (LDL) as a result of a high-fat meal. In order to better understand how an individuals' diet affects arterial plaque deposition via the process of foam cell formation, we measured the acute circulating monocyte activity response after consuming a high-fat meal (85% of daily fat allowance)...
August 29, 2016: Cytometry. Part B, Clinical Cytometry
Teona Roschupkina, Gunnar Juliusson
BACKGROUND: CD4(+) FoxP3(+) regulatory T cells (Tregs) are potent suppressors of activation and proliferation of conventional T cells. Tregs subdivided by their expression of FoxP3 and CD45RA identify clinically important functional subsets. METHODS: We analyzed Treg subpopulations in hematopoietic stem cell harvests (SCH) from 22 allogeneic (matched unrelated and sibling) donors with flow cytometry by their expression of CD45RA, CD127, CD25, and FoxP3 marker combinations...
August 1, 2016: Cytometry. Part B, Clinical Cytometry
Agathe Debliquis, Jimmy Voirin, Inès Harzallah, Maxime Maurer, Felix Lerintiu, Bernard Drénou, Guido Ahle
BACKGROUND: Central nervous system lymphomas are aggressive tumors requiring a prompt diagnosis for successful treatment. Stereotactic biopsy remains the standard procedure, but the time needed for histopathology is usually over 2 days. We evaluated the contribution of cytomorphology and flow cytometry to histopathology of the brain biopsy in particular on the rinse fluid usually removed. METHODS: Eighteen patients with suspected localized brain lymphoma underwent stereotactic brain biopsy...
August 1, 2016: Cytometry. Part B, Clinical Cytometry
J P McCoy
No abstract text is available yet for this article.
September 2016: Cytometry. Part B, Clinical Cytometry
Dharmendra Jain, Pranav Dorwal, Smeeta Gajendra, Amit Pande, Simmi Mehra, Ritesh Sachdev
OBJECTIVE: The aberrant expression of CD5 in both HCL and HCL-v is a very rare event. Although a number of CD5 positive HCL and HCL-v cases have been reported, but these are far and few in between. We aimed to review the reported cases of CD5 positive HCL and its variant. METHOD: We hereby report a case of CD5 positive HCL, with variations in PIK3CA and PDGFRA gene, along with a brief review of literature of the cases of CD5 positive HCL and its variant. RESULTS AND CONCLUSION: The current case was positive for CD103, CD11c, CD25, and CD123 which has led the diagnosis to be of typical HCL...
September 2016: Cytometry. Part B, Clinical Cytometry
Lori Soma, Vivian G Oehler, Cheng Ding, Sindhu Cherian
BACKGROUND: The 2008 WHO is not specific regarding subclassification of chronic myelogenous leukemia (CML) with less than 20% abnormal B lymphoid blasts (ABLB), and suggests patients with ABLB often show rapid progression (Swerdlow, 2008). Recent studies have shown variable outcomes when small abnormal B cell populations are seen by flow cytometry (El Rassi et al., Cancer 2015; 121:872-875; Vrotsos et al., Cytometry B Clin Cytom 2015). METHODS: The hematopathology database was searched (7...
September 2016: Cytometry. Part B, Clinical Cytometry
Thomas R Cimato, Rosemary L Furlage, Alexis Conway, Paul K Wallace
Hematopoietic stem cells are the source of all inflammatory cell types. Discovery of specific cell surface markers unique to human hematopoietic stem (HSC) and progenitor (HSPC) cell populations has facilitated studies of their development from stem cells to mature cells. The specific marker profiles of HSCs and HSPCs can be used to understand their role in human inflammatory diseases. The goal of this study is to simultaneously measure HSCs and HSPCs in normal human venous blood using multicolor flow cytometry...
September 2016: Cytometry. Part B, Clinical Cytometry
Alicia M Hunt, Wendy Shallenberger, Stephen P Ten Eyck, Fiona E Craig
OBJECTIVES: Flow cytometry is an important tool for identification of neoplastic T-cells, but immunophenotypic abnormalities are often subtle and must be distinguished from nonneoplastic subsets. Use of internal control (IC) T-cells in the evaluation for T-cell neoplasms was explored, both as a quality measure and as a reference for evaluating abnormal antigen expression. METHODS: All peripheral blood specimens (3-month period), or those containing abnormal T-cells (29-month period), stained with CD45 V500, CD2 V450, CD3 PE-Cy7, CD7 PE, CD4 Per-CP-Cy5...
September 2016: Cytometry. Part B, Clinical Cytometry
Fanélie Mestrallet, Pierre Sujobert, Clémentine Sarkozy, Alexandra Traverse-Glehen, Evelyne Callet-Bauchu, Jean-Pierre Magaud, Gilles Salles, Lucile Baseggio
BACKGROUND: Altered Toll-like receptor (TLR) expression levels and/or mutations in its signaling pathway (such as MyD88 mutation) contribute to the pathogenesis of lymphoproliferative disorders (LPD). CD180 is an orphan member of the TLR family that modulates the signaling of several TLRs, but only limited studies have evaluated its expression by flow cytometry (FCM) in LPD. METHODS: Using a multiparameter FCM approach, we have assessed CD180 mean fluorescence intensity (MFI) in lymph nodes (LNs) and peripheral blood (PB) samples obtained from patients with follicular lymphoma (FL; LN/PB, n = 44/n = 15), chronic lymphocytic leukemia (CLL, n = 26/n = 21), mantle cell lymphoma (MCL, n = 13/n = 17), and marginal zone lymphoma (MZL, n = 16/n = 12)...
September 2016: Cytometry. Part B, Clinical Cytometry
Eren Gunduz, Serap Sermet, Ahmet Musmul
BACKGROUND: Regulatory T cells (Tregs) are a specialized subpopulation of CD4+ T cells which maintain the immune system homeostasis. They may increase during cancer progression and have been correlated with a worse prognosis in many malignancies. However, the role of Treg cells in lymphoma is debated. METHODS: In this study, we assessed the peripheral blood levels of CD4+ CD25+ FOXP3+ Tregs in newly diagnosed patients with lymphoma and tried to find a relationship with patient characteristics...
September 2016: Cytometry. Part B, Clinical Cytometry
Caroline Mayeur-Rousse, Julien Guy, Laurent Miguet, Sabrina Bouyer, Franck Geneviève, Nelly Robillard, Françoise Solly, Aida Maar, Marie C Bené, Laurent Mauvieux
CD180, a related member of the Toll-like receptor family, is lost or underexpressed at the plasma membrane in circulating cells of various B-cell lymphomas except marginal zone lymphomas (MZL). In order to confirm its clinical relevance in routine analysis, we evaluated prospectively the expression of CD180 in 236 patients from 5 French University Hospital laboratories on behalf of the GEIL. Highly comparable results were obtained in all centers using the EuroFlow standardization protocol. We observed that CD180 median fluorescence (MdFI) was significantly higher in MZL and hairy cell leukaemia (HCL) compared to all other B-cell proliferations (P < 0...
September 2016: Cytometry. Part B, Clinical Cytometry
Bethany D Vallangeon, Christy Tyer, Beverly Williams, Anand S Lagoo
BACKGROUND: Flow cytometric immunophenotyping (FCI) is recognized as a rapid, sensitive, and accurate method for diagnosis of B-cell lymphomas. We observed that FCI failed to identify the clonal B-cell population in several cases of large B-cell lymphoma (DLBCL) when tissue samples were prepared by a commercially available mechanical tissue disaggregation method. We tested a manual tissue disaggregation method and compared it with the mechanical method. METHODS: FCI findings from 51 cases of DLBCL processed with the mechanical tissue disaggregation method, 27 cases processed using the manual method, and 15 cases processed using a combination of both methods were compared...
September 2016: Cytometry. Part B, Clinical Cytometry
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