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Cytometry. Part B, Clinical Cytometry

Marina Garcia-Prat, Daniel Álvarez-Sierra, Aina Aguiló-Cucurull, Sandra Salgado-Perandrés, Sara Briongos-Sebastian, Clara Franco-Jarava, Andrea Martin-Nalda, Roger Colobran, Isabel Montserrat, Manuel Hernández-González, Ricardo Pujol-Borrell, Pere Soler-Palacin, Mónica Martínez-Gallo
BACKGROUND: For the accurate diagnosis of immunodeficiencies is crucial to compare patients' immunology laboratory values with age-sex matched controls, yet there is a paucity of normal values for most populations. OBJECTIVES: To define appropriate reference values of extended lymphocyte subpopulations and T-cell receptor excision circle (TRECs) levels in healthy pediatric donors between 1 month and 18 years of age. METHODS: Extended immunophenotyping values were obtained by analysis of multiparameter flow cytometry panels for the following subpopulations: CD4+ and CD8+ Naive, Effector, Effector Memory and Central Memory, T helper subpopulations and their degrees of activation, T Regulatory cells, Recent Thymic Emigrants (RTE), B Lymphocyte subpopulations (Transitional, Naive, Preswitch-Memory, Switch-Memory, Plasmablasts, CD21low, and Exhausted), and subpopulations for Monocytes, NK cells and Dendritic Cells...
October 17, 2018: Cytometry. Part B, Clinical Cytometry
Ru Feng, Vijaya Raj Bhatt, Kai Fu, Samuel Pirruccello, Ji Yuan
OBJECTIVES: The purpose of this study was to determine whether immunophenotypic profiles detected by flow cytometry are useful in differentiating chronic myelomonocytic leukemia (CMML) from reactive monocytosis, and between CMML subtypes. METHODS: Eight-color flow cytometry was used to immunophenotype blasts, monocytes, and granulocytes in the bone marrow of 34 patients with CMML and 12 patients with reactive monocytosis. RESULTS: Bone marrow myeloblast, promonocyte, and monocyte counts by flow cytometry were significantly higher in the CMML group than in the reactive monocytosis group...
October 17, 2018: Cytometry. Part B, Clinical Cytometry
Nikolaos Gardikas, Myrofora Vikentiou, Evgenia Konsta, Christos K Kontos, Sotirios G Papageorgiou, Aris Spathis, Efthimia Bazani, Anthi Bouchla, Violetta Kapsimali, Katherina Psarra, Periklis Foukas, George Dimitriadis, Vasiliki Pappa
BACKGROUND: Myelodysplastic syndromes (MDS) are clonal hematopoietic stem cell disorders with unknown aetiology. Multiparameter flow cytometry (MFC) is being evaluated for the diagnosis and prognosis of MDS. METHODS: In the present study, five-color MFC was performed on bone marrow aspirates of 50 untreated patients, newly diagnosed with MDS and 27 age matched control samples. Patients were classified according to World Health Organization 2016, International Prognostic Scoring System (IPSS), and Revised IPSS (IPSS-R)...
October 17, 2018: Cytometry. Part B, Clinical Cytometry
Catarina Simões, Isabel Silva, Anabela Carvalho, Sandra Silva, Susana Santos, Gilberto Marques, André Ribeiro, Adriana Roque, José Carda, A B Sarmento-Ribeiro, Maria do Rosário Domingues, Letícia Ribeiro, Artur Paiva
BACKGROUND: Vδ1+ T cells, a subset of γδ T cells, are responsible for innate-like immune responses. Recently, an anti-tumor function mediated by MHC-unrestricted recognition of lipid and stress molecules, has also been described in these cells. This study aimed to quantify and phenotypically characterize circulating Vδ1+ T cells in B cell Chronic Lymphocytic Leukemia (CLL) and Monoclonal B cell lymphocytosis (MBL). METHODS: This study enrolled 58 individuals distributed in five groups: Binet B and C CLL (n = 9), Binet A CLL (n = 26), High count-MBL (n = 10), Low count-MBL (n = 5), and a control group (n = 8)...
October 17, 2018: Cytometry. Part B, Clinical Cytometry
John F Marcelletti, Branimir I Sikic, Larry D Cripe, Elisabeth Paietta
BACKGROUND: Multidrug resistance (MDR) transporter proteins such as P-glycoprotein (P-gp) efflux a variety of chemotherapeutic drugs from acute myeloid leukemia (AML) blasts leading to clinical drug resistance. METHODS: This study examined heterogeneity of MDR functional efflux by AML blasts using two flow cytometry bioassays. Bone marrow specimens (N = 50) from elderly patients with newly diagnosed AML were analyzed for CD34+ blasts with MDR efflux function. Efflux was measured with a fluorescent dye (DiOC2 ) as a surrogate for oncology drugs that are substrates for MDR efflux...
October 17, 2018: Cytometry. Part B, Clinical Cytometry
Thomas Daix, Estelle Guérin, Elsa Tavernier, Jean-Philippe Marsaud, Adélaïde Hacan, François Gauthier, Alessandro Piccardo, Philippe Vignon, Jean Feuillard, Bruno François
BACKGROUND: Similarly, to sepsis, cardiac surgery with cardiopulmonary bypass (CPB) induces major changes in leukocyte subsets. Immature granulocytes (IGs) increase both in sepsis and after open-heart surgery. Secondary infections are a major complication of cardiac surgery with CPB. We hypothesized that the assessment of leukocyte subsets with multicolor flow cytometry (FCM) could help the front-line clinician to better identify patients at high risk of infectious complications in this clinical setting...
October 16, 2018: Cytometry. Part B, Clinical Cytometry
Camila Santos Nobre, Jeovania Almeida Silva, Rafael Henriques Jácomo, Lídia Freire Abdalla Nery, Gustavo Barcelos Barra
BACKGROUND: The cytometric flow osmotic fragility test (FC-OFT) was recently introduced. However, the test is still under development and some variables have not yet been fully tested. METHODS: The osmotic fragility of hereditary spherocytosis (HS) cases and healthy controls were evaluated by FC-OFT using a series of tubes containing decreasing concentrations of NaCl. The analyses were executed in fresh and incubated (37°C for 24 h) blood samples anticoagulated with EDTA and heparin...
October 16, 2018: Cytometry. Part B, Clinical Cytometry
Alba Mora, Rosa Bosch, Carolina Cuellar, Eva Puy Vicente, Laura Blanco, Rodrigo Martino, José M Ubeda, Jorge Sierra, Carol Moreno, Josep Nomdedeu
BACKGROUND: The diagnosis of CLL is supported by a typical morphology and immunophenotype and usually does not present difficulties. Nevertheless, some patients with CLL can show an atypical phenotype, this raising the possibility of a lymphoproliferative disorder other than CLL. It has been recently shown that the expression of CD200 could be a rather consistent marker for CLL. METHODS: The expression of CD200 was investigated in 120 consecutive patients with B-cell chronic lymphoproliferative disorders (B-CLPD) (65 cases diagnosed as typical CLL, 16 atypical CLL, and 39 non-CLL before entering the study) by using multiparametric flow cytometry with four color combinations...
October 16, 2018: Cytometry. Part B, Clinical Cytometry
Pedro Horna, Lynn C Moscinski, Lubomir Sokol, Haipeng Shao
BACKGROUND: Mycosis fungoides (MF) and Sézary Syndrome (SS) are clinically distinct cutaneous T-cell lymphomas with strikingly similar morphologic and phenotypic features. Prior studies have suggested phenotypic differences based on markers of antigen experience, suggesting a different cell of origin. METHODS: Seventy-nine involved peripheral blood or bone marrow samples from 33 patients with SS and 19 patients with MF were studied by 10-color flow cytometry, including CD62L, CD45RA, CCR4, and PD-1...
October 16, 2018: Cytometry. Part B, Clinical Cytometry
Thomas R Cimato, Alexis Conway, Julianne Nichols, Paul K Wallace
BACKGROUND: Circulating hematopoietic progenitors (HPCs) are involved in inflammatory diseases such as atherosclerosis, the immune response to cancer, and disorders of the hematopoietic system. HPC characterization by flow cytometry typically utilizes CD34 in combination with other cell surface markers to identify cell populations that give rise to specific hematopoietic lineages. CD133, also known as prominin-1, is a cell surface protein found in HPCs that has a similar but not interchangeable expression pattern with CD34 for characterization of HPC populations...
October 16, 2018: Cytometry. Part B, Clinical Cytometry
Wei Yang, Xiaochen Yu, Dan Liu, Xiuru Guan
BACKGROUND: The UF-1000i has been widely used in screening urinary sediments. However, the interference factor of the UF-1000i in the screening urinary sediments of pregnant women has not been reported. The aim of the study was to demonstrate that epithelial cells (ECs) cause a high false positive rate of white blood cells (WBCs) by the UF-1000i in pregnant women. METHODS: Urine samples were collected from 207 pregnant women. All samples were measured by the UF-1000i and a microscopic method...
October 10, 2018: Cytometry. Part B, Clinical Cytometry
Fabrízia Rennó Sodero Faulhaber, Gustavo Adolpho Moreira Faulhaber, Natália Aydos Marcondes, Renato Soibelmann Procianoy, Rita C Silveira
BACKGROUND: Jaundice due to indirect hyperbilirubinemia affects more than 60% of neonates and phototherapy is the treatment for severe types. There are no previous studies evaluating the effect of phototherapy on the function of neonates neutrophils. The aim of this study was to assess and compare the function of neutrophils by measuring the expression of neutrophils main surface markers in icteric neonates before and after phototherapy. METHODS: Neonates at a gestational age ≥35 weeks and birth weight ≥2,000 g who met the American Academy of Pediatrics criteria for phototherapy were included...
October 8, 2018: Cytometry. Part B, Clinical Cytometry
Neil Came
No abstract text is available yet for this article.
September 2018: Cytometry. Part B, Clinical Cytometry
Julie Pont, Alice Souvignet, Lydia Campos, Adriana Plesa, Bénédicte Bulabois, Martine Pernollet, Tatiana Raskovalova, Chantal Dumestre-Perard, Jean-Yves Cesbron, Marie-Christine Jacob
BACKGROUND: Studies of normal bone marrow (BM) cell composition by flow cytometry are scarce. Presently, we aimed to quantify 14 cell subsets from infants to elderly patients. METHODS: Cell subsets in BM samples from 180 individuals without morphologically abnormal leukocytes were analyzed using a single combination of eight antibodies: CD3/CD10/CD38/CD19/CD36/CD16/CD34/CD45. RESULTS: By comparison with the Holdrinet score, we first validated the immature granulocyte/neutrophil (IGRA/N) ratio as a readily obtainable criterion of BM sample purity in 145 cases...
September 2018: Cytometry. Part B, Clinical Cytometry
John D M Campbell, Alasdair R Fraser
Cellular therapeutics are a fast-growing, highly innovative area of medicine. This field encompasses well-established immune therapies for infection and cancer, as well as newer cell therapies aimed at regenerating diseased tissue. Flow cytometry is arguably the most important tool in the development of advanced cellular therapeutics and plays a role in many aspects of manufacturing. Quality control of raw materials, assessing rates of cellular growth during complex in vitro culture processes, differentiation status, as well as final product characterization, viability, and product stability are all essential data to be collected and documented...
September 2018: Cytometry. Part B, Clinical Cytometry
Daniel Payne, Ulrika Johansson, David Bloxham, Stephen Couzens, Anthony Carter, Pamela Holtom, Bronia Baker, Mark Hughes, Tara Knill, Tim Milne, Alison Morilla, Ricardo Morilla, David O'Brien, Lisa Thomas
BACKGROUND: A network comprising 11 laboratories aimed to consolidate PNH testing by developing and validating an assay guided by previous guidelines and studies. Network analyses of >20 native samples yielded key findings that were used to create and reshape the final protocol. METHODS: Twenty-seven native samples were distributed to all participating laboratories for blind testing, local analysis, and subsequent re-analysis by a central laboratory. Inter-laboratory clone size precision (coefficient of variation [CV]) was monitored for each sample, and the findings used to refine the test protocol...
September 2018: Cytometry. Part B, Clinical Cytometry
Sa A Wang
No abstract text is available yet for this article.
September 2018: Cytometry. Part B, Clinical Cytometry
Steven R Post, Ginell R Post, Dejan Nikolic, Rebecca Owens, Giovanni Insuasti-Beltran
BACKGROUND: Despite increased usage of multiparameter flow cytometry (MFC) to assess diagnosis, prognosis, and therapeutic efficacy (minimal residual disease, MRD) in plasma cell neoplasms (PCNs), standardization of methodology and data analysis is suboptimal. We investigated the utility of using the mean and median fluorescence intensities (FI) obtained from MFC to objectively describe parameters that distinguish plasma cell (PC) phenotypes. METHODS: In this retrospective study, flow cytometry results from bone marrow aspirate specimens from 570 patients referred to the Myeloma Institute at UAMS were evaluated...
September 2018: Cytometry. Part B, Clinical Cytometry
Yang Zeng, Alan Hiti, Sherry Moranville, Gloria Vicent, Sylvia Chavira, Monika de Arruda Indig, Sharon Graminske, Amanda Boerner, Anna Schmidt, Farzad Oreizy, Angela Chen, Maryam Saleminik, Fred Mosqueda, Anna Lin, Kevin Judge
The BD FACSVia™ system is a novel flow cytometer with improved workflow efficiencies. To evaluate the HLA-B27 application developed on the BD FACSVia system utilizing the BD™ HLA-B27 kit, we conducted a concordance study at three centers to compare with the BD FACSCalibur™ system. Prepared donor samples (n = 594) were analyzed on both the BD FACSVia and BD FACSCalibur for the HLA-B27 assay. Adjudication of HLA-B27 discordant results was performed using the reverse sequence-specific oligonucleotide (rSSO) DNA typing method (LABType® SSO, One Lambda)...
September 2018: Cytometry. Part B, Clinical Cytometry
Joseph A DiGiuseppe, Jolene L Cardinali, William N Rezuke, Dana Pe'er
BACKGROUND: Flow cytometric identification of neoplastic T-cell populations is complicated by the wide range of phenotypic abnormalities in T-cell neoplasia, and the diverse repertoire of reactive T-cell phenotypes. We evaluated whether a recently described clustering algorithm, PhenoGraph, and dimensionality-reduction algorithm, viSNE, might facilitate the identification of abnormal T-cell populations in routine clinical flow cytometric data. METHODS: We applied PhenoGraph and viSNE to peripheral blood mononuclear cells labeled with a single 8-color T/NK-cell antibody combination...
September 2018: Cytometry. Part B, Clinical Cytometry
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