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Cytometry. Part B, Clinical Cytometry

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https://www.readbyqxmd.com/read/29790256/platelet-characteristics-in-patients-with-essential-thrombocytosis
#1
Oliver Heidmann Pedersen, Mads Lamm Larsen, Erik Lerkevang Grove, Peter Buur van Kooten Niekerk, Søren Bønløkke, Peter H Nissen, Steen Dalby Kristensen, Anne-Mette Hvas
BACKGROUND: Essential thrombocytosis (ET) is a myeloproliferative disorder characterized by an increased platelet count. ET is associated with an increased risk of thrombosis, and procoagulant features of the disease may include an increased number of reactive reticulated platelets and an increased aggregation potential. We aimed to explore the association between platelet count, platelet turnover and platelet aggregation in patients with ET. METHODS: We included 24 ET patients who discontinued antiplatelet therapy prior to blood sampling...
May 23, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29747221/flow-cytometry-shows-added-value-in-diagnosing-lymphoma-in-brain-biopsies
#2
Matthijs van der Meulen, Jacoline E C Bromberg, King H Lam, Ruben Dammers, Anton W Langerak, Jeanette K Doorduijn, Johan M Kros, Martin J van den Bent, Vincent H J van der Velden
BACKGROUND: To assess the sensitivity, specificity and turnaround time of flow cytometric analysis on brain biopsies compared to histology plus immunohistochemistry analysis in tumors with clinical suspicion of lymphoma. METHODS: All brain biopsies performed between 2010 and 2015 at our institution and analyzed by both immunohistochemistry and flow cytometry were included in this retrospective study. Immunohistochemistry was considered the gold standard. RESULTS: In a total of 77 biopsies from 71 patients, 49 lymphomas were diagnosed by immunohistochemistry, flow cytometry results were concordant in 71 biopsies (92,2%)...
May 10, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29734502/issue-highlights-may-2018-94b3
#3
Paul K Wallace
No abstract text is available yet for this article.
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29316245/flow-cytometric-aberrancies-in-plasma-cell-myeloma-and-mgus-correlation-with-laboratory-parameters
#4
Sarika Gupta, Nitin J Karandikar, Timothy Ginader, Andrew M Bellizzi, Carol J Holman
BACKGROUND: Multiparametric flow cytometry is a useful tool for diagnosis of plasma cell (PC) dyscrasias and assessment of minimal residual disease in plasma cell myeloma (PCM). However, the immunophenotypic differences between the clonal PCs of PCM and those of monoclonal gammopathy of undetermined significance (MGUS) as well as the correlation of these flow cytometric markers with pertinent laboratory parameters have not been evaluated. METHODS: We retrospectively identified all newly diagnosed treatment-naive PCM and MGUS patients between 09/2014 and 06/2015 who underwent 10-color flow-cytometric evaluation: CD45, CD38, CD138, cKappa, cLambda, CD19, CD27, CD28, CD56, CD117...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29316178/evaluation-of-cd229-as-a-new-alternative-plasma-cell-gating-marker-in-the-flow-cytometric-immunophenotyping-of-monoclonal-gammopathies
#5
Prashant R Tembhare, Sitaram Ghogale, Wilma Tauro, Yajamanam Badrinath, Nilesh Deshpande, Shweta Kedia, Keziah Cherian, Nikhil V Patkar, Gaurav Chatterjee, Sumeet Gujral, Papagudi G Subramanian
BACKGROUND: Current flow-cytometric plasma cell (PC) gating is based on CD138, CD38, and CD45 expression. CD138 is known for variable expression and loss during storage and processing. Introduction of anti-CD38 and anti-CD138 monoclonal-antibody therapies has limited the use of these markers during follow-up. Hence, additional reliable PC-gating markers are required. Recently, CD229 has been claimed as an alternative PC-gating marker. However, these studies are limited to a small cohort of samples...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29244250/human-innate-lymphoid-cells-ilcs-toward-a-uniform-immune-phenotyping
#6
REVIEW
Sara Trabanelli, Alejandra Gomez-Cadena, Bérengère Salomé, Katarzyna Michaud, Domenico Mavilio, Basile Nicolas Landis, Peter Jandus, Camilla Jandus
Helper innate lymphoid cells (ILCs), the most recently identified population of the ILC family, play a fundamental role in the restoration of tissue integrity, in the protection against infiltrating pathogens as well as in tumor immune-surveillance. ILCs have been divided into three main subsets, ILC1, ILC2, and ILC3, that can be specifically activated by different signals coming either indirectly from pathogens or from other cell populations, including cancer cells. Following activation, ILCs are in turn able to promptly secrete a wide range of soluble mediators that modulate effector cell functions...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29244249/flow-cytometric-false-myeloperoxidase-positive-childhood-b-lineage-acute-lymphoblastic-leukemia
#7
Süreyya Savaşan, Steven Buck, Manisha Gadgeel, Ali Gabali
BACKGROUND: Flow cytometric intracellular myeloperoxidase (MPO) staining of leukemic blasts is a useful tool in diagnosis of leukemia subtype. Interpretation of high MPO-positivity can be a diagnostic challenge in B-lineage acute lymphoblastic leukemia (B-ALL). While very few such cases have been reported, high MPO positive B-ALL cases without additional myeloid antigen positivity are suspect and require further investigation. METHODS: Three pediatric cases of B-ALL with strong MPO staining (clone 8E6; Invitrogen) at diagnosis and three others with negative MPO staining were studied by flow cytometry and immunohistochemistry...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29220877/increased-circulating-plasma-cells-detected-by-flow-cytometry-predicts-poor-prognosis-in-patients-with-plasma-cell-myeloma
#8
Mi Hyun Bae, Chan-Jeoung Park, Bo Hyun Kim, Young-Uk Cho, Seongsoo Jang, Dong-Hyun Lee, Eul-Ju Seo, Dok Hyun Yoon, Jung-Hee Lee, Cheolwon Suh
BACKGROUND: Flow cytometry (FC) is a reliable tool for diagnosing and monitoring of plasma cell myeloma (PCM). Recent studies used FC for quantifying plasma cells (PCs) in peripheral blood (PB) using various panels, and an adverse prognostic effect of circulating PCs (cPCs) has been reported. We investigated the prognostic implication of cPCs quantified using a simple panel in patients with PCM. METHODS: Bone marrow (BM) and PB of 85 patients with PCM were analyzed by five-color FC at time of diagnosis...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29220871/role-of-flow-cytometric-immunophenotyping-in-prediction-of-bcr-abl1-gene-rearrangement-in-adult-b-cell-acute-lymphoblastic-leukemia
#9
Francesco Corrente, Silvia Bellesi, Elisabetta Metafuni, Pier Luigi Puggioni, Sara Marietti, Angela Maria Ciminello, Tommaso Za, Federica Sorà, Luana Fianchi, Simona Sica, Valerio De Stefano, Patrizia Chiusolo
We performed a retrospective analysis of 88 adult patients with B-ALL diagnosed in our center by a flow-cytometric assessment. Immunophenotypic expression of leukemic cells was explored by simultaneous evaluation of positivity, percentage of expressing cells and median fluorescence intensity (MFI). BCR/ABL1 fusion transcripts were assessed by RT-PCR analysis and were identified in 36 patients (40.9%). CD10 and CD34 were positive in the totality of BCR/ABL1-positive cases. Patients with gene rearrangement had a greater frequency of CD66c, CD13 and CD33 positivity compared with BCR/ABL1-negative cases...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29160946/panel-proposal-for-the-immunophenotypic-diagnosis-of-hematological-malignancies-a-collaborative-consensus-from-the-groupe-d-etude-immunologique-des-leuc%C3%A3-mies-geil
#10
(no author information available yet)
After a cycle of meetings and discussions, members of the French Groupe d'Etude Immunologique des Leucémies obtained consensus on a series of immunophenotyping panels for hematological malignancies. The rationale of the markers chosen is depicted for each of them. This proposal comes for instruments with 8 or 10 colors. Care was taken to minimize the fluorochromes attached to any given specificity and to leave, whenever possible, some free slots for the cytometrist's choice.
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29144585/therapeutic-potential-of-low-dose-il-2-in-immune-thrombocytopenia-an-analysis-of-3-cases
#11
Jiakui Zhang, Yanjie Ruan, Xuanxuan Xu, Huiping Wang, Qianshan Tao, Jun Lu, Linhuan Xia, Qiuye Zhang, Jeffrey Wang, Yiping Wang, Zhimin Zhai
Immune thrombocytopenia (ITP) is an acquired immune-mediated disorder with regulatory T cells (Tregs) reduction. Recent studies have shown that low-dose interleukin-2 can preferentially induce Treg expansion in vivo, and therefore offers a therapeutic strategy against immune thrombocytopenia. We have demonstrated in a previous study that Tregs and platelet counts significantly improve in an adult with ITP following low-dose IL-2 treatment. Here we report the efficacy of low-dose IL-2 in another three adults with immune thrombocytopenia who failed the first-line treatment...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29059705/low-synovial-double-negative-t-and-%C3%AE-%C3%AE-t-cells-predict-longer-free-disease-survival-in-oligoarticular-jia
#12
Francesco Licciardi, Maria Ceci, Claudia Toppino, Marco Turco, Silvana Martino, Emanuela Ricotti, Francesca Ferro, Davide Montin
BACKGROUND: Oligoarticular juvenile idiopathic arthritis (oJIA) is the most frequent form of chronic arthritis in children; the clinical course is extremely variable. In this study we have characterized by flow cytometry synovial B and T cells subsets in patients with oJIA in order to identify any parameters that could predict a more aggressive course of disease. METHODS: B and T cells from synovial fluid (SF) of 39 patients with oJIA were characterized by flow cytometry...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28888074/early-recovery-of-circulating-immature-b-cells-in-b-lymphoblastic-leukemia-patients-after-cd19-targeted-car-t-cell-therapy-a-pitfall-for-minimal-residual-disease-detection
#13
Wenbin Xiao, Dalia Salem, Catharine S McCoy, Daniel Lee, Nirali N Shah, Maryalice Stetler-Stevenson, Constance M Yuan
BACKGROUND: CD19-targeted chimeric-antigen receptor-modified T-cells (CAR-T) are promising in the treatment of refractory B-lymphoblastic leukemia (B-ALL). Minimal residual disease (MRD) detection by multicolor flow cytometry (FCM) is critical to distinguish B-ALL MRD from regenerating, non-neoplastic B-cell populations. METHODS: FCM was performed on samples from 9 patients with B-ALL treated with CAR-T. RESULTS: All 9 patients showed response to CAR-T...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28865180/automated-and-simplified-identification-of-normal-and-abnormal-plasma-cells-in-multiple-myeloma-by-flow-cytometry
#14
Elina Alaterre, Sébastien Raimbault, Jean-Michel Garcia, Thierry Rème, Guilhem Requirand, Bernard Klein, Jérôme Moreaux
BACKGROUND: Multiple myeloma (MM) is an incurable disease characterized by clonal plasma cell (PC) proliferation within the bone marrow (BM). Next-generation flow cytometry has become the reference tool to follow minimal residual disease (MRD). We developed a new simpler and cheaper flow cytometry method to analyze bone marrow samples in patients with MM. METHODS: To identify and characterize abnormal PCs, we designed a simple panel composed of anti-CD38, antikappa, and antilambda light chain antibodies, combined with two antibody pools with the same fluorophore (anti-CD19 and anti-CD27 for the negative pool and anti-CD56, anti-CD117, and anti-CD200 antibodies for the positive pool)...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28834234/multicolor-flow-cytometric-analysis-of-tlr2-and-tlr9-expression-and-function-in-nk-cells-from-patients-with-anca-associated-vasculitis
#15
Rossana Scrivo, Giovanna Peruzzi, Angelica Gattamelata, Catharina C Gross, Raffaella Carletti, Cira Di Gioia, Jessica Brandt, Roberta Priori, Stefania Morrone, Angela Santoni, Guido Valesini
BACKGROUND: The primary objective of this study was to provide an assessment of NK cells in patients with ANCA-associated vasculitis (AAV). METHODS: Patients were classified based on the presence or absence of ANCAs and compared with healthy controls (HCs). By multiparameter flow cytometry, we evaluated the number and proportion of NK cells (CD3-CD56+) and the CD56dim , CD56bright , CD56dim CD57bright subsets; TLR2 and TLR9 expression; intracellular IFN-γ production upon stimulation with TLR2 and TLR9 ligands; degranulation activity; serum cytokines; immunohistochemical staining of available biopsies...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28802100/influence-of-il-6-il-33-and-tnf-%C3%AE-on-human-mast-cell-activation-lessons-from-single-cell-analysis-by-flow-cytometry
#16
Nathalie Cop, Didier G Ebo, Chris H Bridts, Jessy Elst, Margo M Hagendorens, Christel Mertens, Margaretha A Faber, Luc S De Clerck, Vito Sabato
BACKGROUND: Mechanisms that govern priming and degranulation of human mast cells (MCs) remain elusive. Besides, most of our knowledge is based on experiments from which data only reflect an average of all stimulated cells. This study aims at investigating the effects of proinflammatory cytokines IL-6, IL-33, and TNF-α on IgE-dependent and IgE-independent activation of individual MCs. METHODS: MCs were derived from CD34+ progenitors isolated from 50 mL whole blood from 4 healthy controls and 5 birch pollen allergic patients...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28732145/evaluation-of-primary-mediastinal-large-b-cell-lymphoma-by-flow-cytometry
#17
Sindhu Cherian, Jonathan R Fromm
BACKGROUND: Primary mediastinal large B cell lymphoma (PMLBCL) is a B cell non-Hodgkin lymphoma (B-NHL) that shows morphologic, immunophenotypic, and genetic similarities to classical Hodgkin lymphoma (CHL). We evaluated the neoplastic and reactive infiltrate of PMLBCL by flow cytometry (FC). METHODS: A total of 24 cases of PMLBCL were retrospectively characterized using FC combinations for B-NHL, T-NHL, and CHL. RESULTS: The CHL assay identified the neoplastic population (NP) in all cases, while the B-NHL assay identified the NP in 18 of 24 cases...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28719736/cd326-epcam-testing-by-flow-cytometric-berep4-antibody-is-a-useful-and-rapid-adjunct-to-histopathology
#18
Pranav Dorwal, Helen Moore, Pam Stewart, Barbara Harrison, Julie Monaghan
Flow cytometry has been traditionally used to diagnose leukaemia and lymphoma in peripheral blood, bone marrow, body fluids, and tissue samples. The diagnosis of a malignant epithelial tumour is usually made by correlation between histopathologic appearance and the use of immunohistochemical staining. A CE approved BerEP4 antibody (anti-EpCAM, CD326) is available for flow cytometric testing, but has been evaluated predominantly in body fluids in the current literature. In this study, we have evaluated the performance of this antibody in detecting the presence of epithelial cells in tissue samples which have traditionally been reported as CD45 negative cells by flow cytometry...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28718205/bcl-2-maturation-pattern-in-t-cells-distinguishes-thymic-neoplasm-hyperplasia-t-lymphoblastic-lymphoma-and-reactive-lymph-nodes
#19
Nicholas Ward, Junaid Baqai, Alexandra Zehnpfennig, Nancy Fine, James Huang, Marc D Smith
Anterior mediastinal biopsies consisting predominantly of small lymphocytes can be a diagnostic challenge, especially in small core biopsies. In these cases, immunophenotyping is often employed using flow cytometry, and/or immunohistochemistry. However, due the overlap in T-cell phenotype between thymic neoplasm/hyperplasia (THY), T-lymphoblastic lymphoma (T-LBL), and reactive lymph nodes (RLN), biopsies consisting predominantly of T-cells may still be difficult to differentiate. Previous studies have shown a specific CD3/bcl-2 staining pattern in thymic T cells of humans and mice using flow cytometry...
May 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28718199/the-clec12a-receptor-marks-human-basophils-potential-implications-for-minimal-residual-disease-detection-in-acute-myeloid-leukemia
#20
Marie Toft-Petersen, Anne Stidsholt Roug, Trine Plesner, Lene Ebbesen, Gordon D Brown, Line Nederby
BACKGROUND: The transmembrane receptor C-type lectin domain family 12, member A (CLEC12A) is known to be highly expressed on monocytes and neutrophils and is a reliable leukemia associated marker in acute myeloid leukemia. Consequently, detailed knowledge of the various normal cell types expressing this receptor is essential. We have observed CLEC12A to be expressed on CD45lowSSClowCD14-CD123+ basophils in peripheral blood (PB) and in this study, we aimed at verifying this observation and further delineate the CD45lowSSClowCD14-CD123 + CLEC12A+ subpopulation...
May 2018: Cytometry. Part B, Clinical Cytometry
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