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Cytometry. Part B, Clinical Cytometry

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https://www.readbyqxmd.com/read/29220877/increased-circulating-plasma-cells-detected-by-flow-cytometry-predicts-poor-prognosis-in-patients-with-plasma-cell-myeloma
#1
Mi Hyun Bae, Chan-Jeoung Park, Bo Hyun Kim, Young-Uk Cho, Seongsoo Jang, Dong-Hyun Lee, Eul-Ju Seo, Dok Hyun Yoon, Jung-Hee Lee, Cheolwon Suh
BACKGROUND: Flow cytometry (FC) is a reliable tool for diagnosing and monitoring of plasma cell myeloma (PCM). Recent studies used FC for quantifying plasma cells (PCs) in peripheral blood (PB) using various panels, and an adverse prognostic effect of circulating PCs (cPCs) has been reported. We investigated the prognostic implication of cPCs quantified using a simple panel in patients with PCM. METHODS: Bone marrow (BM) and PB of 85 patients with PCM were analyzed by five-color FC at time of diagnosis...
December 8, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29220871/role-of-flow-cytometric-immunophenotyping-in-prediction-of-bcr-abl1-gene-rearrangement-in-adult-b-cell-acute-lymphoblastic-leukemia
#2
Francesco Corrente, Silvia Bellesi, Elisabetta Metafuni, Pier Luigi Puggioni, Sara Marietti, Angela Maria Ciminello, Tommaso Za, Federica Sorà, Luana Fianchi, Simona Sica, Valerio De Stefano, Patrizia Chiusolo
We performed a retrospective analysis of 88 adult patients with B-ALL diagnosed in our center by a flow-cytometric assessment. Immunophenotypic expression of leukemic cells was explored by simultaneous evaluation of positivity, percentage of expressing cells and median fluorescence intensity (MFI). Presence of BCR/ABL1 fusion transcripts were assessed by RT-PCR analysis and were identified in 36 patients (40.9%). CD10 and CD34 were positive in the totality of BCR/ABL1-positive cases. Patients with gene rearrangement had a greater frequency of CD66c, CD13 and CD33 positivity compared with BCR/ABL1-negative cases...
December 8, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29220870/quantitative-assessment-of-informative-immunophenotypic-markers-increases-the-diagnostic-value-of-immunophenotyping-in-mature-cd5-positive-b-cell-neoplasms
#3
Starostka David, Kriegova Eva, Kudelka Milos, Mikula Peter, Zehnalova Sarka, Radvansky Martin, Papajik Tomas, Kolacek David, Chasakova Katerina, Talianova Hana
BACKGROUND: The data on the clinical utility of the quantitative assessment of immunophenotypes in distinguishing mature CD5-positive B-cell neoplasms is limited. The study aim was to assess the diagnostic value of the quantitative assessment of a panel of 18 markers and to identify the most informative ones. METHODS: The immunophenotype of the neoplastic population was determined in diagnostic specimens from 188 patients. BD FACSCanto II flow cytometer and FACSDiva software were used to analyse the positivity/negativity and mean fluorescence intensity (MFI) of the surface expression of 18 markers...
December 8, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29205788/normal-variation-of-bone-marrow-b-cell-precursors-according-to-age-reference-ranges-for-studies-in-myelodysplastic-syndromes-in-brazil
#4
I Lorand-Metze, A L Longhini, G Oliveira-Duarte, R P Correia, M C Santos-Silva, M Yamamoto, A F Sandes, A F Oliveira, E X Souto, Mrv Ikoma, F G Pereira-Cunha, M Beltrame, Konradin Metze
BACKGROUND: Normal B lymphoid maturation occurs in bone marrow (BM) throughout life, but immature B-cell progenitors (BCPs) are more numerous in children than in adults. To assess the normal values according to age became important as BCPs are decreased in myelodysplastic syndromes and have been considered an important diagnostic and prognostic feature in these clonal disorders. METHODS: in a multicenter retrospective study from the Brazilian Group of Flow Cytometry we analyzed the variation of BCPs in normal BM according to age and technical peculiarities of each laboratory...
December 2, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29171939/initial-flow-cytometric-evaluation-of-the-clearllab-lymphoid-screen
#5
B D Hedley, G Cheng, J Luider, W Kern, G Lozanski, I Chin-Yee, L E Lowes, M Keeney, D Careaga, R Magari, L Tejidor
INTRODUCTION: Flow cytometric immunophenotyping (FCI) is an integral part in the diagnosis and classification of hematologic malignancies. FCI results also influence therapeutic decisions and disease prognosis. ClearLLab LS is a 12-antibody 10-color cocktail provided in dry format designed as a screen for patients suspected of having hematolymphoid disease. METHODS: A blinded comparison between ClearLLab LS, (CD8-FITC,Kappa-FITC,CD4-PE,Lambda-PE,CD19-ECD,CD56-PE-Cy5...
November 24, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29160946/collective-publication-panel-proposal-for-the-immunophenotypic-diagnosis-of-hematological-malignancies-a-collaborative-consensus-from-the-groupe-d-etude-immunologique-des-leuc%C3%A3-mies-geil
#6
https://www.readbyqxmd.com/read/29144585/therapeutic-potential-of-low-dose-il-2-in-immune-thrombocytopenia-an-analysis-of-3-cases
#7
Jiakui Zhang, Yanjie Ruan, Xuanxuan Xu, Huiping Wang, Qianshan Tao, Jun Lu, Linhuan Xia, Qiuye Zhang, Jeffrey Wang, Yiping Wang, Zhimin Zhai
Immune thrombocytopenia (ITP) is an acquired immune-mediated disorder with regulatory T cells (Tregs) reduction. Recent studies have shown that low-dose interleukin-2 can preferentially induce regulatory T cell expansion in vivo, and therefore offers a therapeutic strategy against immune thrombocytopenia. We have demonstrated in a previous study that Tregs and platelet counts significantly improve in an adult with ITP following low-dose IL-2 treatment. Here we report the efficacy of low-dose interleukin-2 in another three adults with immune thrombocytopenia who failed the first-line treatment...
November 16, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29108126/high-sensitivity-of-the-hematoflow%C3%A2-solution-for-chronic-myelomonocytic-leukemia-screening
#8
Romain Vazquez, Mikael Roussel, Bouchra Badaoui, Nicolas Freynet, Sihem Tarfi, Eric Solary, Dorothée Selimoglu-Buet, Orianne Wagner-Ballon
BACKGROUND: Accumulation of classical monocytes CD14(++) CD16(-) (also called MO1) ≥94% can accurately distinguish chronic myelomonocytic leukemia (CMML) from reactive monocytosis. The HematoFlow™ solution, able to quantify CD16 negative monocytes, could be a useful tool to manage monocytosis which remains a common issue in routine laboratories. METHODS: Classical monocytes were quantified from 153 whole blood samples collected on EDTA using both flow cytometry methods, either MO1 percentage determination by the multiparameter assay previously published and regarded here as the reference method, or CD16 negative monocyte percentage determination by the means of HematoFlow™...
November 6, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29072803/the-presence-of-f-cells-with-a-fetal-phenotype-in-adults-with-hemoglobinopathies-limits-the-utility-of-flow-cytometry-for-quantitation-of-fetomaternal-hemorrhage
#9
Jad Othman, Daniel Orellana, Lin Selina Chen, Megan Russell, Teh-Liane Khoo
BACKGROUND: Detection and quantitation of fetomaternal hemorrhage (FMH) can be difficult in patients with pre-existing elevations of HbF, such as those with hemoglobinopathies. The aim of this study was to evaluate the utility of dual-color flow cytometry with the Fetal Cell Count Kit (FCCK) in differentiating adult and fetal HbF in this population, as compared to flow cytometry (FC) using HbF alone. METHODS: Peripheral blood was obtained from normal adults and patients with hemoglobinopathies (β-thalassemia and sickle cell disease), including a small number of pregnant females...
October 26, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29059705/low-synovial-double-negative-t-and-%C3%AE-%C3%AE-t-cells-predict-longer-free-disease-survival-in-oligoarticular-jia
#10
Francesco Licciardi, Maria Ceci, Claudia Toppino, Marco Turco, Silvana Martino, Davide Montin
Background Oligoarticular juvenile idiopathic arthritis (oJIA) is the most frequent form of chronic arthritis in children; the clinical course is extremely variable. In this study we have characterized by flow cytometry synovial B and T cells subsets in patients with oJIA in order to identify any parameters that could predict a more aggressive course of disease. Methods B and T cells from synovial fluid (SF) of 39 patients with oJIA were characterized by flow cytometry. In 22 patients SF was analysed at the onset of the disease (GroupA), in 17 SF was analysed at articular relapse (Group B)...
October 23, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29024518/cd5-b-lymphoproliferative-disorder-with-subsequent-development-of-plasma-cell-leukaemia-diagnostic-and-aetiologic-reasoning
#11
Evdoxia Gounari, Georgia Kaiafa, Triantafyllia Koletsa, Vasiliki Tsavdaridou, Ioannis Kostopoulos, Lilian Toptsi, Lemonia Skoura
BACKGROUND: Plasma cell myeloma (PCM) has been sporadically reported to occur simultaneously or subsequently to mature B lymphoproliferative disorders (LPDs), predominantly chronic lymphocytic leukaemia (CLL). METHODS: We describe the clinical and laboratory findings of a 69-year-old male patient who developed plasma cell leukaemia (PCL) 8 years after an initial diagnosis of a low stage CD5+ B LPD and 3 years after treatment for LPD. RESULTS: The transition from a clinically indolent B LPD to an aggressive PCM was documented by bone marrow (BM) biopsy, while flow cytometric (FC) immunophenotyping conferred additional information by disclosing the co-existence of both disorders in BM and the presence of abnormal monotypic PCs in peripheral blood above PCL levels...
October 10, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29024461/reproducible-diagnosis-of-chronic-lymphocytic-leukemia-by-flow-cytometry-an-european-research-initiative-on-cll-eric-european-society-for-clinical-cell-analysis-escca-harmonisation-project
#12
Andy C Rawstron, Karl-Anton Kreuzer, Asha Soosapilla, Martin Spacek, Olga Stehlikova, Peter Gambell, Neil McIver-Brown, Neus Villamor, Katherina Psarra, Maria Arroz, Raffaella Milani, Javier de la Serna, M Teresa Cedena, Ozren Jaksic, Josep Nomdedeu, Carol Moreno, Gian Matteo Rigolin, Antonio Cuneo, Preben Johansen, Hans E Johnsen, Richard Rosenquist, Carsten Utoft Niemann, Wolfgang Kern, David Westerman, Marek Trneny, Stephen Mulligan, Michael Doubek, Sarka Pospisilova, Peter Hillmen, David Oscier, Michael Hallek, Paolo Ghia, Emili Montserrat
The diagnostic criteria for CLL rely on morphology and immunophenotype. Current approaches have limitations affecting reproducibility and there is no consensus on the role of new markers. The aim of this project was to identify reproducible criteria and consensus on markers recommended for the diagnosis of CLL. ERIC/ESCCA members classified 14 of 35 potential markers as "required" or "recommended" for CLL diagnosis, consensus being defined as >75% and >50% agreement, respectively. An approach to validate "required" markers using normal peripheral blood was developed...
October 10, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28985649/peripheral-blood-mononuclear-cell-immunophenotyping-in-fibrodysplasia-ossificans-progressiva-patients-evidence-for-monocyte-dnam1-up-regulation
#13
Genny Del Zotto, Francesca Antonini, Irma Azzari, Claudio Ortolani, Gino Tripodi, Francesca Giacopelli, Serena Cappato, Lorenzo Moretta, Roberto Ravazzolo, Renata Bocciardi
Background Fibrodysplasia Ossificans Progressiva (FOP) is a rare genetic disorder caused by sporadic heterozygous mutations in ACVR1 gene which progressively leads to severe heterotopic ossification. FOP is characterized by episodic flare-ups triggered by different factors such as viral infections, tissue injuries, vaccinations or occurring without a recognizable cause. The sporadic course of the disease, the documented presence of an important inflammatory reaction in early lesions and the partial response to corticosteroids support the idea that the immune system, and in particular the innate component, may play a role in FOP pathogenesis...
October 6, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28980766/cancer-related-mrna-expression-analysis-using-a-novel-flow-cytometry-based-assay
#14
REVIEW
Barbara Depreter, Jan Philippé, Magali Meul, Barbara Denys, Karl Vandepoele, Barbara De Moerloose, Tim Lammens
BACKGROUND: Cancer-related gene expression data mostly originate from unfractionated bulk samples, leading to "expression averaging" of heterogeneous populations. Multicolor flow cytometry (FCM) may distinguish heterogeneous populations based on the phenotypic characterization of single-cells, but is not applicable for RNA targets. Here, we evaluated the PrimeFlow™ RNA assay, a novel FCM-based assay designed to measure gene expressions, in two cancer entities with high and low RNA target levels...
October 5, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28960773/flow-cytometry-assessment-of-intracellular-btk-expression
#15
LETTER
Natália Aydos Marcondes, Flavo Beno Fernandes, Bianca Michel Spindler, Gustavo Adolpho Moreira Faulhaber
No abstract text is available yet for this article.
September 28, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29077268/issue-highlights-november-2017-92-b6
#16
Luc Kestens, Frank Mandy
No abstract text is available yet for this article.
November 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28321976/a-5-color-flow-cytometric-method-for-extended-8-part-leukocyte-differential
#17
Julien Guy, Orianne Wagner-Ballon, Olivier Pages, François Bailly, Jessica Borgeot, Marie-C Béné, Marc Maynadié
OBJECTIVES: Microscopic leukocyte differentials display many drawbacks. Several single 5 to 8-color tubes using multiparameter flow cytometry (MFC) are able to provide extended differentials with sequential gating-based analysis strategies. We investigated a new 5-color MFC method to perform an extended 8-part differential with a simplified gating strategy. METHODS: Whole blood was stained with a combination of antibodies including HLA-DR-FITC/CD19-PE/CD45-ECD/CD16-PC5 + CD71-PC5/CD5-PC7...
November 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28296063/choosing-a-new-cd4-technology-can-statistical-method-comparison-tools-influence-the-decision
#18
Lesley E Scott, Luc Kestens, Kovit Pattanapanyasat, Kasma Sukapirom, Wendy S Stevens
BACKGROUND: Method comparison tools are used to determine the accuracy, precision, agreement, and clinical relevance of a new or improved technology versus a reference technology. Guidelines for the most appropriate method comparison tools as well as their acceptable limits are lacking and not standardized for CD4 counting technologies. METHODS: Different method comparison tools were applied to a previously published CD4 dataset (n = 150 data pairs) evaluating five different CD4 counting technologies (TruCOUNT, Dual Platform, FACSCount, Easy CD4, CyFlow) on a single specimen...
November 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/27684937/image-analysis-of-neutrophil-nuclear-morphology-learning-about-phenotypic-range-and-its-reliable-analysis-from-patients-with-pelger-hu%C3%A3-t-anomaly-and-treated-with-colchicine
#19
Nele Schnipper, Hans H Stassen, Tilmann Kallinich, Karl Sperling, Katrin Hoffmann
The nuclear morphology of neutrophils depends on different endogenous and exogenous factors, which can lead to hypo- or hypersegmentation of the normally 2-4 segmented nucleus. Hyposegmentation can be due to mutations in the LBR-gene (Pelger-Huët-Anomaly) or can be induced, for example, by colchicine treatment. The range of this phenotypic variation is known as "norm of reaction," which can be of major relevance for clinical diagnosis and therapeutic intervention. In this project, we studied the norm of reaction in 26 subjects with 0-3 wild type LBR alleles...
November 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/27406947/thirty-five-years-of-cd4-t-cell-counting-in-hiv-infection-from-flow-cytometry-in-the-lab-to-point-of-care-testing-in-the-field
#20
REVIEW
L Kestens, F Mandy
CD4 T-cell counting was introduced in clinical laboratories shortly after the discovery of the human immune deficiency virus (HIV) in the early eighties. In western clinical laboratories, improvements in the CD4 T-cell counting methods were mainly driven by progress in the field of flow cytometry and immunology. In contrast, the development of dedicated CD4 T-cell counting technologies were needs driven. When antiretroviral treatment (ART) was made available on a large scale by international Acquired Immune Deficiency Syndrome (AIDS) relief programs to HIV+ patients living in low income countries in 2003, there was a distinct need for simplified and affordable CD4 T-cell counting technologies...
November 2017: Cytometry. Part B, Clinical Cytometry
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