journal
MENU ▼
Read by QxMD icon Read
search

Cytometry. Part B, Clinical Cytometry

journal
https://www.readbyqxmd.com/read/29790256/platelet-characteristics-in-patients-with-essential-thrombocytosis
#1
Oliver Heidmann Pedersen, Mads Lamm Larsen, Erik Lerkevang Grove, Peter Buur van Kooten Niekerk, Søren Bønløkke, Peter H Nissen, Steen Dalby Kristensen, Anne-Mette Hvas
BACKGROUND: Essential thrombocytosis (ET) is a myeloproliferative disorder characterized by an increased platelet count. ET is associated with an increased risk of thrombosis, and procoagulant features of the disease may include an increased number of reactive reticulated platelets and an increased aggregation potential. We aimed to explore the association between platelet count, platelet turnover and platelet aggregation in patients with ET. METHODS: We included 24 ET patients who discontinued antiplatelet therapy prior to blood sampling...
May 23, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29747221/flow-cytometry-shows-added-value-in-diagnosing-lymphoma-in-brain-biopsies
#2
Matthijs van der Meulen, Jacoline E C Bromberg, King H Lam, Ruben Dammers, Anton W Langerak, Jeanette K Doorduijn, Johan M Kros, Martin J van den Bent, Vincent H J van der Velden
BACKGROUND: To assess the sensitivity, specificity and turnaround time of flow cytometric analysis on brain biopsies compared to histology plus immunohistochemistry analysis in tumors with clinical suspicion of lymphoma. METHODS: All brain biopsies performed between 2010 and 2015 at our institution and analyzed by both immunohistochemistry and flow cytometry were included in this retrospective study. Immunohistochemistry was considered the gold standard. RESULTS: In a total of 77 biopsies from 71 patients, 49 lymphomas were diagnosed by immunohistochemistry, flow cytometry results were concordant in 71 biopsies (92,2%)...
May 10, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29734502/clinical-cytometry-b-issue-highlights-may-2018-94b3
#3
Paul K Wallace
No abstract text is available yet for this article.
May 7, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29704326/polyclonal-b-cell-lymphocytosis-report-of-three-cases
#4
Natália Aydos Marcondes, Laiana Schneider, Bianca Michel Spindler, Flavo Beno Fernandes, Gustavo Adolpho Moreira Faulhaber
BACKGROUND: Persistent polyclonal B-cell lymphocytosis (PPBL) is a rare benign condition characterized by a polyclonal B-cell lymphocytosis with binucleated lymphocytes. We hereby report three cases of PPBL. METHODS: Flow cytometry immunophenotyping was performed in peripheral blood samples from three patients with clinical suspicion of lymphoproliferative disease. RESULTS: Case 1 was a female middle-aged smoker; Case 2 was an elderly male; and Case 3 was a non-smoker female...
April 27, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29669178/the-utility-of-multiparametric-flow-cytometry-in-the-detection-of-primary-effusion-lymphoma-pel
#5
Jacobo Galán, Isaac Martin, Itziar Carmona, José María Rodriguez-Barbero, Esperanza Cuadrado, Luis García-Alonso, José Antonio García-Vela
Primary effusion lymphoma (PEL) is a rare B cell lymphoproliferative disorder that arises predominantly in body cavities causing malignant effusions. The incidence of PEL is very low, accounting for approximately 4% of all HIV-associated non-Hodgkin lymphomas. PEL has also been described in elderly patients and after solid-organ transplantation. It is associated in all cases with human herpes virus 8 (HHV8). We describe a case of PEL in a 88-year-old HIV-negative woman who presented with progressive dyspnea and moderate right-sided pleural effusion without significant lymphadenopathies or other effusions...
April 18, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29577600/quantification-of-number-of-cd38-sites-on-bone-marrow-plasma-cells-in-patients-with-light-chain-amyloidosis-and-smoldering-multiple-myeloma
#6
Katharina Kriegsmann, Tobias Dittrich, Brigitte Neuber, Mohamed H S Awwad, Ute Hegenbart, Hartmut Goldschmidt, Jens Hillengass, Dirk Hose, Anja Seckinger, Carsten Müller-Tidow, Anthony D Ho, Stefan Schönland, Michael Hundemer
BACKGROUND: Recent approaches in multiple myeloma (MM) treatment have targeted CD38. As antigen expression levels on plasma cells (PCs) were demonstrated to affect response to monoclonal antibody (mAb) treatment, a precise characterization of PC phenotype is warranted. METHODS: Anti-CD38 mAb (isatuximab) was tested for antibody-dependent cellular cytotoxicity (ADCC) in MM cell lines. Quantification of the number of sites (NOS) of CD38 on bone marrow PCs and other immune cells obtained from light chain (AL) amyloidosis (n = 46) and smoldering multiple myeloma (SMM) patients (n = 19) was performed with two different quantitative flow cytometry (QFCM) applications...
March 25, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29573142/development-of-an-unbiased-semi-automated-approach-for-classifying-plasma-cell-immunophenotype-following-multicolor-flow-cytometry-of-bone-marrow-aspirates
#7
Steven R Post, Ginell R Post, Dejan Nikolic, Rebecca Owens, Giovanni Insuasti-Beltran
BACKGROUND: Despite increased usage of multiparameter flow cytometry (MFC) to assess diagnosis, prognosis, and therapeutic efficacy (minimal residual disease, MRD) in plasma cell neoplasms (PCNs), standardization of methodology and data analysis is suboptimal. We investigated the utility of using the mean and median fluorescence intensities (FI) obtained from MFC to objectively describe parameters that distinguish plasma cell (PC) phenotypes. METHODS: In this retrospective study, flow cytometry results from bone marrow aspirate specimens from 570 patients referred to the Myeloma Institute at UAMS were evaluated...
March 24, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29534323/a-simple-and-effective-method-for-flow-cytometric-study-of-lymphoid-malignancies-using-needle-core-biopsy-specimens
#8
Pei-Dong Chi, Natalie S Freed, Laura Wake, Cynthia Page, Lynette M Smith, Catalina Amador, Hina Naushad Qureishi, Timothy C Greiner, Scott J Rodig, Samuel J Pirruccello, Ji Yuan, Kai Fu
OBJECTIVES: We developed a simple and effective rinsing technique (RT) of needle biopsies to produce cell suspensions for flow cytometry (FCM) and evaluated whether the RT is comparable to the conventional tissue cell suspension (TCS) technique. METHODS: We retrieved 93 needle core biopsy cases employing the RT for FCM and 25 needle biopsy cases using TCS for FCM. RESULTS: The diagnostic concordance between the FCM results and the morphologic diagnoses of both groups was compared...
March 13, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29489061/obituary
#9
Bruno Brando
No abstract text is available yet for this article.
March 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29438583/issue-highlights-march-2018
#10
Bruno Brando
No abstract text is available yet for this article.
March 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28885784/changes-in-tonsil-b-cell-phenotypes-and-ebv-receptor-expression-in-children-under-5-years-old
#11
Eric M Wohlford, Paul C Baresel, Joel R Wilmore, Anthony J Mortelliti, Carrie B Coleman, Rosemary Rochford
BACKGROUND: Palatine tonsils are principally B cell organs that are the initial line of defense against many oral pathogens, as well as the site of infection for others. While the size of palatine tonsils changes greatly in the first five years of life, the cellular changes during this period are not well studied. Epstein Barr virus (EBV) is a common orally transmitted virus that infects tonsillar B cells. Naïve B cells are thought to be the target of primary infection with EBV in vivo, suggesting that they are targeted by the virus...
March 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28480599/laboratory-accuracy-improvement-in-the-uk-neqas-leucocyte-immunophenotyping-immune-monitoring-program-an-eleven-year-review-via-longitudinal-mixed-effects-modeling
#12
John Bainbridge, Wes Rountree, Raul Louzao, John Wong, Liam Whitby, Thomas N Denny, David Barnett
BACKGROUND: The United Kingdom National External Quality Assessment Service (UK NEQAS) for Leucocyte Immunophenotyping Immune Monitoring Programme, provides external quality assessment (EQA) to non-U.S. laboratories affiliated with the NIH NIAID Division of AIDS (DAIDS) clinical trials networks. Selected laboratories are required to have oversight, performance monitoring, and remediation undertaken by Immunology Quality Assessment (IQA) staff under the DAIDS contract. We examined whether laboratory accuracy improves with longer EQA participation and whether IQA remediation is effective...
March 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28475275/a-qa-program-for-mrd-testing-demonstrates-that-systematic-education-can-reduce-discordance-among-experienced-interpreters
#13
Michael Keeney, Brent L Wood, Benjamin D Hedley, Joseph A DiGiuseppe, Maryalice Stetler-Stevenson, Elisabeth Paietta, Gerard Lozanski, Adam C Seegmiller, Bruce W Greig, Aaron C Shaver, Lata Mukundan, Howard R Higley, Caroline C Sigman, Gary Kelloff, J Milburn Jessup, Michael J Borowitz
BACKGROUND: Minimal residual disease (MRD) in B lymphoblastic leukemia (B-ALL) by flow cytometry is an established prognostic factor used to adjust treatment in most pediatric therapeutic protocols. MRD in B-ALL has been standardized by the Children's Oncology Group (COG) in North America, but not routine clinical labs. The Foundation for National Institutes of Health sought to harmonize MRD measurement among COG, oncology groups, academic, community and government, laboratories. METHODS: Listmode data from post-induction marrows were distributed from a reference lab to seven different clinical FCM labs with variable experience in B-ALL MRD...
March 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28378895/monitoring-cd49d-receptor-occupancy-a-method-to-optimize-and-personalize-natalizumab-therapy-in-multiple-sclerosis-patients
#14
Joan Puñet-Ortiz, José Vicente Hervás-García, Aina Teniente-Serra, Antonio Cano-Orgaz, Maria José Mansilla, Bibiana Quirant-Sánchez, Juan Navarro-Barriuso, Marco A Fernández-Sanmartín, Silvia Presas-Rodríguez, Cristina Ramo-Tello, Eva María Martínez-Cáceres
BACKGROUND: In natalizumab-treated relapsing-remitting MS (RRMS) patients, various extended interval dosing strategies are under evaluation to minimize severe treatment-associated side effects, mainly progressive multifocal leukoencephalopathy development. Up to now, it has not been presented any approach, even in form of assay design, to determine the optimal percentage of CD49d receptor occupancy (RO) associated with a favorable clinical, radiological, and immunological response. METHODS: A multiparametric quantitative flow cytometry method was settled to measure CD49d RO on peripheral blood lymphocytes...
March 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28257592/visualization-of-cell-composition-and-maturation-in-the-bone-marrow-using-10-color-flow-cytometry-and-radar-plots
#15
Katayoon Jafari, Anne Tierens, Amr Rajab, Rumina Musani, André Schuh, Anna Porwit
BACKGROUND: The enormous potential of complex data files generated by 10-color flow cytometry (FC) is hindered by the requirement for exhaustive manual gating and the complexity of multidimensional data visualization. We propose a model using radar plots (RPs), to improve FC data visualization by capturing multidimensionality and integration of FC findings. METHOD: We analysed 12 normal/reactive bone marrow (N/R BM) samples and 12 BM samples from patients with myelodysplasia (MDS) with 10-color FC...
March 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28188679/quantitative-flow-cytometry-concerns-and-recommendations-in-clinic-and-research
#16
REVIEW
Olga Mizrahi, Eliran Ish Shalom, Michal Baniyash, Yair Klieger
BACKGROUND: Quantitative flow cytometry (QFCM) can be an important element within the developing toolbox for clinical diagnostics which relies on precise and rapid tests that provide a conclusive answer for physicians. The FC technology combines all of these features. Until recently, this imperative discipline was based on qualitative assessments of cell populations. However, due to the enormous advancement in FC technology, which allows the quantification of a number of antigens on cell surface and within the cells by units of median fluorescence intensity (MFI), this method becomes meaningful and fits the clinical needs...
March 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28187524/phenotypic-characterization-of-trisomy-12-monoclonal-b-cell-lymphocytosis
#17
Marc Sorigue, Clara Maluquer, Jordi Junca
BACKGROUND: Trisomy 12 chronic lymphocytic leukemia (CLL) is phenotypically different from the rest of CLL cytogenetic subgroups. However, it is unknown whether this is also the case for trisomy 12 CLL-phenotype monoclonal B-cell lymphocytosis (MBL). METHODS: We analyzed the expression of several markers in a series of 89 cytogenetically characterized MBL (including 17 trisomy 12 cases). Additionally, we compared the expression of these markers between trisomy 12 MBL, trisomy 12 CLL and a series of cases with trisomy 12 but fulfilling only 3 of the 5 Moreau CLL diagnostic criteria...
March 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28124496/flow-cytometric-characteristics-of-extrathymic-thymocytes-in-adenoid-tissue-a-case-report-and-comparison-to-normal-thymus-and-thymoma
#18
Ji Yuan, Vasantha L Gali, Deborah A Perry, Kai Fu, Hina Qureishi, Catalina Amador-Ortiz, Timothy Greiner, Samuel J Pirruccello
BACKGROUND: Normal thymocyte precursors in secondary lymphoid organs have previously been described. It is important to recognize normal thymocyte precursors by flow cytometry to differentiate them from T-cell lymphoblastic leukemia. METHODS: A 3-year-old boy status 2 years postallogenic cardiac transplant underwent adenoidectomy to exclude post-transplant lymphoproliferative disorder. Microscopic, immunohistochemical, and flow cytometry analyses of the adenoid were performed...
March 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28103645/prognostic-impact-of-lymphocyte-subpopulations-in-peripheral-blood-after-hematopoietic-stem-cell-transplantation-for-hematologic-malignancies
#19
Sang Hyuk Park, Chan-Jeoung Park, Borae G Park, Mi-Hyun Bae, Bo-Hyun Kim, Young-Uk Cho, Seongsoo Jang, Ae-Ja Park, Dae-Young Kim, Jung-Hee Lee, Je-Hwan Lee, Kyoo-Hyung Lee
BACKGROUND: We prospectively evaluated prognostic value of lymphocyte subpopulations in peripheral blood of allogeneic hematopoietic stem cell transplant (HSCT) recipients. METHODS: 113 allogeneic HSCT (47 sibling matched, 37 unrelated matched, 29 haploidentical)-performed patients diagnosed as AML (n = 66), ALL (n = 28), and MDS (n = 19) were prospectively enrolled. 14 lymphocyte subpopulations were quantified by flow cytometry of PB at specific time-points after HSCT, and their prognostic impacts were analyzed...
March 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28052584/activated-platelet-derived-and-leukocyte-derived-circulating-microparticles-and-the-risk-of-thrombosis-in-heparin-induced-thrombocytopenia-a-role-for-pf4-bearing-microparticles
#20
Elena Campello, Claudia M Radu, Elena Duner, Anna M Lombardi, Luca Spiezia, Raffaele Bendo, Silvia Ferrari, Paolo Simioni, Fabrizio Fabris
BACKGROUND: Though the presence of platelets-derived microparticles (MPs) have previously been described in heparin-induced thrombocytopenia (HIT), the mechanism of thrombosis in HIT remains poorly understood. We aimed to assess the presence and origin of MPs in patients with HIT and their possible contribution to HIT with thrombosis (HITT). METHODS: Forty-five patients with HIT and 45 matched hospitalized patients with not confirmed HIT (HIT-negative) were enrolled...
March 2018: Cytometry. Part B, Clinical Cytometry
journal
journal
40908
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"