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International Journal of Biological Sciences

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https://www.readbyqxmd.com/read/28090191/blockage-of-epithelial-to-mesenchymal-transition-and-upregulation-of-let-7b-are-critically-involved-in-ursolic-acid-induced-apoptosis-in-malignant-mesothelioma-cell
#1
Eun Jung Sohn, Gunho Won, Jihyun Lee, Sang Wook Yoon, Ilho Lee, Hee Jeong Kim, Sung-Hoon Kim
Malignant pleural mesothelioma (MPN), which is caused by asbestos exposure, is one of aggressive lung tumors. In the present study, we elucidated the anti-tumor mechanism of ursolic acid in malignant mesotheliomas. Ursolic acid significantly exerted cytotoxicity in a time and dose dependent manner in H28, H2452 and MSTO-211H mesothelioma cells and inhibited cell proliferation by colony formation assay in a dose-dependent fashion. Also, ursolic acid treatment accumulated the sub-G1 population, attenuated the expression of procapase 9, cyclin D1, pAKT, p-glycogen synthase kinase 3-alpha/beta (pGSK3α/β), β-catenin and nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) and also cleaved caspase 3 and poly (ADP-ribose) polymerase (PARP) in mesothelioma cells...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994520/evaluation-of-breast-cancer-stem-cells-and-intratumor-stemness-heterogeneity-in-triple-negative-breast-cancer-as-prognostic-factors
#2
Fang Yang, Lulu Cao, Zijia Sun, Juan Jin, Hehui Fang, Wenwen Zhang, Xiaoxiang Guan
Triple-negative breast cancer (TNBC) is a tumor subtype with aggressive behavior and poor clinical outcome for lacking effective therapies. Breast cancer stem cells (BCSCs) have been suggested to have tumor-initiating properties, but it remains unclear whether their presence contributes to the increased aggressiveness and poor prognosis of TNBC. Also, the breast cancers display frequent inter- and intra-tumor heterogeneity, which adds the complexity in diagnosis and predicting prognosis. Here we investigated the clinical relevance and prognostic value of the BCSC markers, CD44(+)/CD24(-), aldehyde dehydrogenase family 1 member A1 (ALDH1A1) and CD133 in 88 TNBC cases...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994519/anticancer-effects-of-a-new-sirt-inhibitor-mhy2256-against-human-breast-cancer-mcf-7-cells-via-regulation-of-mdm2-p53-binding
#3
Eun Young Park, Youngwoo Woo, Seong Jin Kim, Do Hyun Kim, Eui Kyung Lee, Umasankar De, Kyeong Seok Kim, Jaewon Lee, Jee H Jung, Ki-Tae Ha, Wahn Soo Choi, In Su Kim, Byung Mu Lee, Sungpil Yoon, Hyung Ryong Moon, Hyung Sik Kim
The sirtuins (SIRTs), a family of NAD(+)-dependent class III histone deacetylase, are involved in various biological processes including cell survival, division, senescence, and metabolism via activation of the stress-response pathway. Recently, inhibition of SIRTs has been considered a promising anticancer strategy, but their precise mechanisms of action are not well understood. In particular, the relevance of p53 to SIRT-induced effects has not been fully elucidated. We investigated the anticancer effects of a novel SIRT inhibitor, MHY2256, and its efficacy was compared to that of salermide in MCF-7 (wild-type p53) and SKOV-3 (null-type p53) cells...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994518/signal-transduction-mechanism-for-glucagon-induced-leptin-gene-expression-in-goldfish-liver
#4
Ai-Fen Yan, Ting Chen, Shuang Chen, Dong-Sheng Tang, Fang Liu, Xiao Jiang, Wen Huang, Chun-Hua Ren, Chao-Qun Hu
Leptin is a peripheral satiety hormone that also plays important roles in energy homeostasis in vertebrates ranging from fish to mammals. In teleost fish, however, the regulatory mechanism for leptin gene expression still remains unclear. In this study, we found that glucagon, a key hormone in glucose homeostasis, was effective at elevating the leptin-AI and leptin-AII transcript levels in goldfish liver via both in vivo intraperitoneal injection and in vitro cells incubation approaches. The responses of leptin-AI and leptin-AII mRNA to glucagon treatment were highly comparable...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994517/cyclooxygenase-2-in-tumor-associated-macrophages-promotes-metastatic-potential-of-breast-cancer-cells-through-akt-pathway
#5
Lu Gan, Zhu Qiu, Jing Huang, Yunhai Li, Hongyan Huang, Tingxiu Xiang, Jingyuan Wan, Tianli Hui, Yong Lin, Hongzhong Li, Guosheng Ren
Tumor-associated macrophages (TAMs) promote cancer development and progression by releasing various cytokines and chemokines. Previously, we have found that the number of COX-2(+) TAMs was associated with lymph node metastasis in breast cancer. However, the mechanism remains enigmatic. In this study, we show that COX-2 in breast TAMs enhances the metastatic potential of breast cancer cells. COX-2 in TAMs induces MMP-9 expression and promotes epithelial-mesenchymal transition (EMT) in breast cancer cells. In addition, COX-2/PGE2 induces IL-6 release in macrophages...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994516/genes-involved-in-angiogenesis-and-mtor-pathways-are-frequently-mutated-in-asian-patients-with-pancreatic-neuroendocrine-tumors
#6
Wen-Chi Chou, Po-Han Lin, Yi-Chen Yeh, Yi-Ming Shyr, Wen-Liang Fang, Shin-E Wang, Chun-Yu Liu, Peter Mu-Hsin Chang, Ming-Han Chen, Yi-Ping Hung, Chung-Pin Li, Yee Chao, Ming-Huang Chen
Introduction: To address the issue of limited data on and inconsistent findings for genetic alterations in pancreatic neuroendocrine tumors (pNETs), we analyzed sequences of known pNET-associated genes for their impact on clinical outcomes in a Taiwanese cohort. Methods: Tissue samples from 40 patients with sporadic pNETs were sequenced using a customized sequencing panel that analyzed 43 genes with either an established or potential association with pNETs. Genetic mutations and clinical outcomes were analyzed for potential associations...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994515/dose-dependent-effects-of-strontium-ranelate-on-ovariectomy-rat-bone-marrow-mesenchymal-stem-cells-and-human-umbilical-vein-endothelial-cells
#7
Xiaojing Guo, Silong Wei, Mengmeng Lu, Zhengwei Shao, Jiayu Lu, Lunguo Xia, Kaili Lin, Derong Zou
In clinic, strontium ranelate (SrR) is a useful drug to treat osteoporosis by orally taken method, but some side effect appeared in recent years. The aim of this study is to evaluate the effectiveness and safety of SrR on cells by direct application, to study the possibility of local application of this drug. Qualitative ALP staining, quantitative ALP activity assay, alizarin red staining, realtime PCR and westernblot assay were used to evaluate the osteogenesis ability of SrR under normal or osteogenic induction environment of ovariectomy bone marrow mesenchymal stem cells (OVX-BMSCs)...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994514/the-correlation-between-parp1-and-brca1-in-ar-positive-triple-negative-breast-cancer
#8
Jiayan Luo, Juan Jin, Fang Yang, Zijia Sun, Wenwen Zhang, Yaqin Shi, Jing Xu, Xiaoxiang Guan
Triple-negative breast cancer (TNBC) lacks estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER-2) expression and thus cannot benefit from conventional hormonal or anti-HER2 targeted therapies. Anti-androgen therapy has shown a certain effect on androgen receptor (AR) positive TNBC. The emerging researches have proved that poly (ADP-ribose) polymerase (PARP) inhibitor is effective in BRCA1-deficient breast cancers. We demonstrated that combination of AR antagonist (bicalutamide) and PARP inhibitor (ABT-888) could inhibit cell viability and induce cell apoptosis significantly whatever in vitro or in vivo setting in AR-positive TNBC...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994513/lis1-regulates-osteoclastogenesis-through-modulation-of-m-scf-and-rankl-signaling-pathways-and-cdc42
#9
Shiqiao Ye, Toshifumi Fujiwara, Jian Zhou, Kottayil I Varughese, Haibo Zhao
We have previously reported that depletion of LIS1, a key regulator of microtubules and cytoplasmic dynein motor complex, in osteoclast precursor cells by shRNAs attenuates osteoclastogenesis in vitro. However, the underlying mechanisms remain unclear. In this study, we show that conditional deletion of LIS1 in osteoclast progenitors in mice led to increased bone mass and decreased osteoclast number on trabecular bone. In vitro mechanistic studies revealed that loss of LIS1 had little effects on cell cycle progression but accelerated apoptosis of osteoclast precursor cells...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994512/exosomes-derived-from-human-endothelial-progenitor-cells-accelerate-cutaneous-wound-healing-by-promoting-angiogenesis-through-erk1-2-signaling
#10
Jieyuan Zhang, Chunyuan Chen, Bin Hu, Xin Niu, Xiaolin Liu, Guowei Zhang, Changqing Zhang, Qing Li, Yang Wang
Chronic skin wounds represent one of the most common and disabling complications of diabetes. Endothelial progenitor cells (EPCs) are precursors of endothelial cells and can enhance diabetic wound repair by facilitating neovascularization. Recent studies indicate that the transplanted cells exert therapeutic effects primarily via a paracrine mechanism and exosomes are an important paracrine factor that can be directly used as therapeutic agents for regenerative medicine. However, application of exosomes in diabetic wound repair has been rarely reported...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994511/autophagy-activation-protects-shock-wave-induced-renal-tubular-epithelial-cell-apoptosis-may-through-modulation-of-akt-gsk-3%C3%AE-pathway
#11
Qingzhi Long, Xiang Li, Hui He, Dalin He
PURPOSE: Extracorporeal shock wave lithotripsy (ESWL) is well documented to exert destructive effect to renal cells and its mechanism is not clear. Autophagy is one of cell basic response for stressful conditions and it is important to determine cell's fate. The aim of this study is to elucidate the role of autophagy in the process of shock wave-induced renal cells injury. METHODS: NRK-52E cell, a rat renal tubular epithelial cell, was exposed to shock wave at the voltage of 14KV...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994510/pathological-characterization-of-ifnar-mice-infected-with-bluetongue-virus-serotype-4
#12
Alejandro Marín-López, Roberto Bermúdez, Eva Calvo-Pinilla, Sandra Moreno, Alejandro Brun, Javier Ortego
Bluetongue virus (BTV) replicates in lymphoid tissues where infected mononuclear leukocytes secrete proinflammatory and vasoactive mediators that can contribute to bluetongue (BT) pathogenesis. Using the well-characterized IFNAR(-/-) mice animal model, we have now studied the histopathology and dynamics of leukocyte populations in different target tissues (spleen, thymus, and lung) during BTV-4 infection by histological and immunohistochemical techniques. The spleen and thymus of BTV-4 infected mice showed severe lymphoid depletion on H&E stained sections...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994509/mir-99a-and-mir-491-regulate-cisplatin-resistance-in-human-gastric-cancer-cells-by-targeting-capns1
#13
Yajie Zhang, Wenxia Xu, Pan Ni, Aiping Li, Jianwei Zhou, Shan Xu
Cisplatin is the first-line agent utilized for the clinical treatment of a wide variety of solid tumors including gastric cancer. However, the intrinsic or acquired cisplatin resistance is often occurred in patients with gastric cancer and resulted in failure of cisplatin therapy. In order to investigate if miRNA involves in cisplatin resistance of human gastric cancer, we first screened and compared the expression of miRNAs between cisplatin resistant gastric cancer cell lines SGC-7901/DDP and BGC-823/DDP and their sensitive parental cells by miRNAs microarray and followed by analysis of 2D-GE/MS to identify their target proteins...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994508/crispr-cas9-from-genome-editing-to-cancer-research
#14
REVIEW
Si Chen, Heng Sun, Kai Miao, Chu-Xia Deng
Cancer development is a multistep process triggered by innate and acquired mutations, which cause the functional abnormality and determine the initiation and progression of tumorigenesis. Gene editing is a widely used engineering tool for generating mutations that enhance tumorigenesis. The recent developed clustered regularly interspaced short palindromic repeats-CRISPR-associated 9 (CRISPR-Cas9) system renews the genome editing approach into a more convenient and efficient way. By rapidly introducing genetic modifications in cell lines, organs and animals, CRISPR-Cas9 system extends the gene editing into whole genome screening, both in loss-of-function and gain-of-function manners...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994507/catalpol-protects-pre-myelinating-oligodendrocytes-against-ischemia-induced-oxidative-injury-through-erk1-2-signaling-pathway
#15
Qiyan Cai, Teng Ma, Chengren Li, Yanping Tian, Hongli Li
The vulnerability of pre-myelinating oligodendrocytes (PreOLs) to ischemic injury plays an important role in the pathogenesis and progression of perinatal white matter injury. Although oxidative stress is thought to be a major pathogenic mechanism predisposing the PreOLs to injury, no effective therapies have been identified to date. The present study aimed to investigate the direct protective effects of catalpol, a potent antioxidant and free radical scavenger, on ischemia-induced oxidative damage in PreOLs and to explore whether the ERK1/2 signaling pathway contributed to the protection provided by catalpol...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994506/distributed-features-of-vimentin-containing-neural-precursor-cells-in-olfactory-bulb-of-sod1g93a-transgenic-mice-a-study-about-resource-of-endogenous-neural-stem-cells
#16
Chunyan Tang, Lei Zhu, Weiming Gan, Huiting Liang, Jiao Li, Jie Zhang, Xiong Zhang, Yi Lu, Renshi Xu
No any effective treatments can prevent from the motor neuron degeneration in amyotrophic lateral sclerosis (ALS) at present. In order to modulating the endogenous neural precursor cells (NPCs) to repairing the degenerative motor neurons in ALS, we studied the alteration of endogenous vimentin-containing NPCs in olfactory bulb (OB) at the different stages of SOD1 wlid-type and G93A transgenic mice. The results showed that the vimentin-containing cells (VCCs) were mainly distributed in the glomerular layer (Gl), the accessory OB (AOB), the OB core, the granular cell layer (GRO) and the mitral cell layer (MI)+the internal plexiform layer (IPL) of the OB of adult mice...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27877091/conserved-chemosensory-proteins-in-the-proboscis-and-eyes-of-lepidoptera
#17
Jiao Zhu, Immacolata Iovinella, Francesca Romana Dani, Yu-Ling Liu, Ling-Qiao Huang, Yang Liu, Chen-Zhu Wang, Paolo Pelosi, Guirong Wang
Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are endowed with several different functions besides being carriers for pheromones and odorants. Based on a previous report of a CSP acting as surfactant in the proboscis of the moth Helicoverpa armigera, we revealed the presence of orthologue proteins in two other moths Plutella xylostella and Chilo suppressalis, as well as two butterflies Papilio machaon and Pieris rapae, using immunodetection and proteomic analysis. The unusual conservation of these proteins across large phylogenetic distances indicated a common specific function for these CSPs...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27877090/genetic-variant-in-flavin-containing-monooxygenase-3-alters-lipid-metabolism-in-laying-hens-in-a-diet-specific-manner
#18
Jing Wang, Cheng Long, Haijun Zhang, Yanan Zhang, Hao Wang, Hongyuan Yue, Xiaocui Wang, Shugeng Wu, Guanghai Qi
Genetic variant T329S in flavin-containing monooxygenase 3 (FMO3) impairs trimethylamine (TMA) metabolism in birds. The TMA metabolism that under complex genetic and dietary regulation, closely linked to cardiovascular disease risk. We determined whether the genetic defects in TMA metabolism may change other metabolic traits in birds, determined whether the genetic effects depend on diets, and to identify genes or gene pathways that underlie the metabolic alteration induced by genetic and diet factors. We used hens genotyped as FMO3 c...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27877089/insights-into-the-nanog-gene-a-propeller-for-stemness-in-primitive-stem-cells
#19
REVIEW
Wei Zhang, Yi Sui, Jun Ni, Tao Yang
Self-renewal and pluripotency are two major characteristics of embryonic stem cells (ESCs) that allow ESCs to maintain stem cell population, and differentiate into multiple types of adult tissues. Nanog is the key transcription factor that controls both self-renewal and pluripotency of ESCs. Similarly, cancer stem cells (CSCs) are capable of preserving population and initiating new tumor development by self-renewal. Expression of Nanog family proteins can be increased in many types of cancer which is correlated with tumor outcomes...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27877088/aloe-emodin-suppresses-hypoxia-induced-retinal-angiogenesis-via-inhibition-of-hif-1%C3%AE-vegf-pathway
#20
Jianming Wu, Xiao Ke, Wei Wang, Hongcheng Zhang, Na Ma, Wei Fu, Manxi Zhao, Xiaoping Gao, Xiaofeng Hao, Zhirong Zhang
Background: Aloe-emodin (AE) has been reported to possess the antiangiogenic effect on laser induced choroidal neovascularization. AE inhibits the vessel formation in the zebrafish embryos. However, it is still unclear whether AE can alleviate neovascularization. Here, we investigated the inhibitory effect of AE on the hypoxia-induced retinal neovascularization and the possible mechanisms. Methods: We established a vascular endothelial growth factor (VEGF) secretion model under chemical induced hypoxia by exposure of 150 µM CoCl2 to the ARPE-19 cells, then treated the cells with different concentrations of AE (0...
2016: International Journal of Biological Sciences
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