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International Journal of Biological Sciences

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https://www.readbyqxmd.com/read/28123352/mir-15a-3p-and-mir-16-1-3p-negatively-regulate-twist1-to-repress-gastric-cancer-cell-invasion-and-metastasis
#1
Tao Wang, Jingjing Hou, Zengpeng Li, Zihan Zheng, Jie Wei, Dan Song, Tao Hu, Qiao Wu, James Y Yang, Jian-Chun Cai
MicroRNAs are a novel class of gene regulators that function as oncogenes or tumor suppressors. In our current study, we investigated the role of miR-15a-3p and miR-16-1-3p in the regulation of Twist1 expression and EMT process. Our bioinformatics analysis suggested that on the 3' UTR of Twist1, there are two conserved miRNA recognition sites for miR-15a-3p and miR-16-1-3p respectively. Interestingly, overexpression of miR-15a-3p and miR-16-1-3p significantly suppressed the activity of luciferase reporter containing Twist1-3' UTR, reduced mRNA and protein level of EMT related genes such as TWIST1, N-cadherin, α-SMA and Fibronectin, and repressed MMP9 and MMP2 activity, as well as cell migration and invasion...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28123351/involvement-of-rictor-mtorc2-in-cardiomyocyte-differentiation-of-mouse-embryonic-stem-cells-in-vitro
#2
Bei Zheng, Jiadan Wang, Leilei Tang, Chao Tan, Zhe Zhao, Yi Xiao, Renshan Ge, Danyan Zhu
Rictor is a key regulatory/structural subunit of the mammalian target of rapamycin complex 2 (mTORC2) and is required for phosphorylation of Akt at serine 473. It plays an important role in cell survival, actin cytoskeleton organization and other processes in embryogenesis. However, the role of Rictor/mTORC2 in the embryonic cardiac differentiation has been uncovered. In the present study, we examined a possible link between Rictor expression and cardiomyocyte differentiation of the mouse embryonic stem (mES) cells...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28123350/the-effect-of-estradiol-on-the-growth-plate-chondrocytes-of-limb-and-spine-from-postnatal-mice-in-vitro-the-role-of-estrogen-receptor-and-estradiol-concentration
#3
Sheng Shi, Shuang Zheng, Xin-Feng Li, Zu-De Liu
Objectives: Skeletal development is a complex process. Little is known about the different response of limb or spine growth plate chondrocytes (LGP or SGP) to the estrogen level and the role of estrogen receptor (ER) during postnatal stage. Methods: LGP and SGP chondrocytes were isolated from 50 one-week mice and treated with different concentrations of 17β-estradiol. Cell viability was measured by cell counting kit-8 (CCK-8). The expression of collagen II and X were evaluated by real-time PCR and Western blotting...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28123349/metadata-analysis-of-phanerochaete-chrysosporium-gene-expression-data-identified-common-cazymes-encoding-gene-expression-profiles-involved-in-cellulose-and-hemicellulose-degradation
#4
Ayyappa Kumar Sista Kameshwar, Wensheng Qin
In literature, extensive studies have been conducted on popular wood degrading white rot fungus, Phanerochaete chrysosporium about its lignin degrading mechanisms compared to the cellulose and hemicellulose degrading abilities. This study delineates cellulose and hemicellulose degrading mechanisms through large scale metadata analysis of P. chrysosporium gene expression data (retrieved from NCBI GEO) to understand the common expression patterns of differentially expressed genes when cultured on different growth substrates...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28123348/inhibition-of-mirna-210-reverses-nicotine-induced-brain-hypoxic-ischemic-injury-in-neonatal-rats
#5
Lei Wang, Jun Ke, Yong Li, Qinyi Ma, Chiranjib Dasgupta, Xiaohui Huang, Lubo Zhang, DaLiao Xiao
Maternal tobacco use in pregnancy increases the risk of neurodevelopmental disorders and neurobehavioral deficits in postnatal life. The present study tested the hypothesis that perinatal nicotine exposure exacerbated brain vulnerability to hypoxic-ischemic (HI) injury in neonatal rats through up-regulation of miR-210 expression in the developing brain. Nicotine was administered to pregnant rats via subcutaneous osmotic minipumps. Experiments of HI brain injury were performed in 10-day-old pups. Perinatal nicotine treatment significantly decreased neonatal body and brain weights, but increased the brain to body weight ratio...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28123347/forsythoside-a-exerts-antipyretic-effect-on-yeast-induced-pyrexia-mice-via-inhibiting-transient-receptor-potential-vanilloid-1-function
#6
Cuiling Liu, Hongchang Su, Hongye Wan, Qingxia Qin, Xuan Wu, Xiangying Kong, Na Lin
Transient receptor potential vanilloid 1 (TRPV1) is a non-selective cation channel gated by noxious heat, playing major roles in thermoregulation. Forsythoside A (FT-A) is the most abundant phenylethanoid glycosides in Fructus Forsythiae, which has been prescribed as a medicinal herb for treating fever in China for a long history. However, how FT-A affects pyrexia and what is the underlying molecular mechanism remain largely unknown. Here we found that FT-A exerted apparent antipyretic effect through decreasing the levels of prostaglandin E2 (PGE2) and interleukin 8 (IL-8) in a dose-dependent fashion on the yeast induced pyrexia mice...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28123346/the-efficiency-analysis-of-thrombolytic-rt-pa-combined-with-intravascular-interventional-therapy-in-patients-with-acute-basilar-artery-occlusion
#7
Zhao Xianxian, Yue Chengsong, Mei Qiang, Wei Fei, Shen Lin, Ding Huiyan, Gong Zili
In order to further optimize the treatment strategy for the patients with acute basilar artery occlusion, we were dedicated to study the therapeutic effects and influential factors in the process of treated basilar artery occlusion with thrombolytic combined vascular interventional therapy. 75 patients with acute basilar artery occlusion treated with arterial thrombolytic therapy were analyzed retrospectively. In accordance with the discharge records of patients, their short-term curative effect with 24-hour treatment and 14-days treatment were evaluated...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28123345/the-protective-effect-of-alpha-7-nicotinic-acetylcholine-receptor-activation-on-critical-illness-and-its-mechanism
#8
REVIEW
Chao Ren, Ya-Lin Tong, Jun-Cong Li, Zhong-Qiu Lu, Yong-Ming Yao
Critical illnesses and injuries are recognized as major threats to human health, and they are usually accompanied by uncontrolled inflammation and dysfunction of immune response. The alpha 7 nicotinic acetylcholine receptor (α7nAchR), which is a primary receptor of cholinergic anti-inflammatory pathway (CAP), exhibits great benefits for critical ill conditions. It is composed of 5 identical α7 subunits that form a central pore with high permeability for calcium. This putative structure is closely associated with its functional states...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28123344/deformed-skull-morphology-is-caused-by-the-combined-effects-of-the-maldevelopment-of-calvarias-cranial-base-and-brain-in-fgfr2-p253r-mice-mimicking-human-apert-syndrome
#9
Fengtao Luo, Yangli Xie, Wei Xu, Junlan Huang, Siru Zhou, Zuqiang Wang, Xiaoqing Luo, Mi Liu, Lin Chen, Xiaolan Du
Apert syndrome (AS) is a common genetic syndrome in humans characterized with craniosynostosis. Apert patients and mouse models showed abnormalities in sutures, cranial base and brain, that may all be involved in the pathogenesis of skull malformation of Apert syndrome. To distinguish the differential roles of these components of head in the pathogenesis of the abnormal skull morphology of AS, we generated mouse strains specifically expressing mutant FGFR2 in chondrocytes, osteoblasts, and progenitor cells of central nervous system (CNS) by crossing Fgfr2(+/P253R-Neo) mice with Col2a1-Cre, Osteocalcin-Cre (OC-Cre), and Nestin-Cre mice, respectively...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28123343/let-7a-is-an-antihypertrophic-regulator-in-the-heart-via-targeting-calmodulin
#10
Xin Zhou, Fei Sun, Shenjian Luo, Wei Zhao, Ti Yang, Guiye Zhang, Ming Gao, Renzhong Lu, You Shu, Wei Mu, Yanan Zhuang, Fengzhi Ding, Chaoqian Xu, Yanjie Lu
Background: MicroRNAs (miRNAs) have been emerged as important regulator in a multiple of cardiovascular disease, including arrhythmia, cardiac hypertrophy and fibrosis, and myocardial infarction. The aim of this study was to investigate whether miRNA let-7a has antihypertrophic effects in angiotensin II (AngII)-induced cardiac hypertrophy. Methods: Neonatal rat ventricular myocytes (NRVMs) were exposed to AngII for 36 h as a cellular model of hypertrophy; subcutaneous injection of AngII for 2 weeks was used to establish a mouse model of cardiac hypertrophy in vivo study...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28123342/microrna-135a-regulates-apoptosis-induced-by-hydrogen-peroxide-in-rat-cardiomyoblast-cells
#11
Ning Liu, Yong-Feng Shi, Hong-Ying Diao, Yang-Xue Li, Yan Cui, Xian-Jing Song, Xin Tian, Tian-Yi Li, Bin Liu
Oxidative stress and apoptosis are the most important pathologic features of ischemic heart disease. Recent research has indicated that microRNAs (miRs) play an essential role in apoptosis. However, whether miRs might regulate B cell lymphoma-2 (Bcl-2) protein in apoptosis during ischemic heart disease is still unclear. The aim of this study, therefore, was to confirm the regulation of microRNA-135a (miR-135a) in oxidative stress injuries induced by hydrogen peroxide (H2O2) in rat cardiomyoblast cells H9c2...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28123341/downregulation-of-lipin-1-induces-insulin-resistance-by-increasing-intracellular-ceramide-accumulation-in-c2c12-myotubes
#12
Shujuan Huang, Suling Huang, Xi Wang, Qingli Zhang, Jia Liu, Ying Leng
Dysregulation of lipid metabolism in skeletal muscle is involved in the development of insulin resistance. Mutations in lipin-1, a key lipid metabolism regulator leads to significant systemic insulin resistance in fld mice. However, the function of lipin-1 on lipid metabolism and insulin sensitivity in skeletal muscle is still unclear. Herein we demonstrated that downregulation of lipin-1 in C2C12 myotubes by siRNA transfection suppressed insulin action, characterized by reduced insulin stimulated Akt phosphorylation and glucose uptake...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28090191/blockage-of-epithelial-to-mesenchymal-transition-and-upregulation-of-let-7b-are-critically-involved-in-ursolic-acid-induced-apoptosis-in-malignant-mesothelioma-cell
#13
Eun Jung Sohn, Gunho Won, Jihyun Lee, Sang Wook Yoon, Ilho Lee, Hee Jeong Kim, Sung-Hoon Kim
Malignant pleural mesothelioma (MPN), which is caused by asbestos exposure, is one of aggressive lung tumors. In the present study, we elucidated the anti-tumor mechanism of ursolic acid in malignant mesotheliomas. Ursolic acid significantly exerted cytotoxicity in a time and dose dependent manner in H28, H2452 and MSTO-211H mesothelioma cells and inhibited cell proliferation by colony formation assay in a dose-dependent fashion. Also, ursolic acid treatment accumulated the sub-G1 population, attenuated the expression of procapase 9, cyclin D1, pAKT, p-glycogen synthase kinase 3-alpha/beta (pGSK3α/β), β-catenin and nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) and also cleaved caspase 3 and poly (ADP-ribose) polymerase (PARP) in mesothelioma cells...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994520/evaluation-of-breast-cancer-stem-cells-and-intratumor-stemness-heterogeneity-in-triple-negative-breast-cancer-as-prognostic-factors
#14
Fang Yang, Lulu Cao, Zijia Sun, Juan Jin, Hehui Fang, Wenwen Zhang, Xiaoxiang Guan
Triple-negative breast cancer (TNBC) is a tumor subtype with aggressive behavior and poor clinical outcome for lacking effective therapies. Breast cancer stem cells (BCSCs) have been suggested to have tumor-initiating properties, but it remains unclear whether their presence contributes to the increased aggressiveness and poor prognosis of TNBC. Also, the breast cancers display frequent inter- and intra-tumor heterogeneity, which adds the complexity in diagnosis and predicting prognosis. Here we investigated the clinical relevance and prognostic value of the BCSC markers, CD44(+)/CD24(-), aldehyde dehydrogenase family 1 member A1 (ALDH1A1) and CD133 in 88 TNBC cases...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994519/anticancer-effects-of-a-new-sirt-inhibitor-mhy2256-against-human-breast-cancer-mcf-7-cells-via-regulation-of-mdm2-p53-binding
#15
Eun Young Park, Youngwoo Woo, Seong Jin Kim, Do Hyun Kim, Eui Kyung Lee, Umasankar De, Kyeong Seok Kim, Jaewon Lee, Jee H Jung, Ki-Tae Ha, Wahn Soo Choi, In Su Kim, Byung Mu Lee, Sungpil Yoon, Hyung Ryong Moon, Hyung Sik Kim
The sirtuins (SIRTs), a family of NAD(+)-dependent class III histone deacetylase, are involved in various biological processes including cell survival, division, senescence, and metabolism via activation of the stress-response pathway. Recently, inhibition of SIRTs has been considered a promising anticancer strategy, but their precise mechanisms of action are not well understood. In particular, the relevance of p53 to SIRT-induced effects has not been fully elucidated. We investigated the anticancer effects of a novel SIRT inhibitor, MHY2256, and its efficacy was compared to that of salermide in MCF-7 (wild-type p53) and SKOV-3 (null-type p53) cells...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994518/signal-transduction-mechanism-for-glucagon-induced-leptin-gene-expression-in-goldfish-liver
#16
Ai-Fen Yan, Ting Chen, Shuang Chen, Dong-Sheng Tang, Fang Liu, Xiao Jiang, Wen Huang, Chun-Hua Ren, Chao-Qun Hu
Leptin is a peripheral satiety hormone that also plays important roles in energy homeostasis in vertebrates ranging from fish to mammals. In teleost fish, however, the regulatory mechanism for leptin gene expression still remains unclear. In this study, we found that glucagon, a key hormone in glucose homeostasis, was effective at elevating the leptin-AI and leptin-AII transcript levels in goldfish liver via both in vivo intraperitoneal injection and in vitro cells incubation approaches. The responses of leptin-AI and leptin-AII mRNA to glucagon treatment were highly comparable...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994517/cyclooxygenase-2-in-tumor-associated-macrophages-promotes-metastatic-potential-of-breast-cancer-cells-through-akt-pathway
#17
Lu Gan, Zhu Qiu, Jing Huang, Yunhai Li, Hongyan Huang, Tingxiu Xiang, Jingyuan Wan, Tianli Hui, Yong Lin, Hongzhong Li, Guosheng Ren
Tumor-associated macrophages (TAMs) promote cancer development and progression by releasing various cytokines and chemokines. Previously, we have found that the number of COX-2(+) TAMs was associated with lymph node metastasis in breast cancer. However, the mechanism remains enigmatic. In this study, we show that COX-2 in breast TAMs enhances the metastatic potential of breast cancer cells. COX-2 in TAMs induces MMP-9 expression and promotes epithelial-mesenchymal transition (EMT) in breast cancer cells. In addition, COX-2/PGE2 induces IL-6 release in macrophages...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994516/genes-involved-in-angiogenesis-and-mtor-pathways-are-frequently-mutated-in-asian-patients-with-pancreatic-neuroendocrine-tumors
#18
Wen-Chi Chou, Po-Han Lin, Yi-Chen Yeh, Yi-Ming Shyr, Wen-Liang Fang, Shin-E Wang, Chun-Yu Liu, Peter Mu-Hsin Chang, Ming-Han Chen, Yi-Ping Hung, Chung-Pin Li, Yee Chao, Ming-Huang Chen
Introduction: To address the issue of limited data on and inconsistent findings for genetic alterations in pancreatic neuroendocrine tumors (pNETs), we analyzed sequences of known pNET-associated genes for their impact on clinical outcomes in a Taiwanese cohort. Methods: Tissue samples from 40 patients with sporadic pNETs were sequenced using a customized sequencing panel that analyzed 43 genes with either an established or potential association with pNETs. Genetic mutations and clinical outcomes were analyzed for potential associations...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994515/dose-dependent-effects-of-strontium-ranelate-on-ovariectomy-rat-bone-marrow-mesenchymal-stem-cells-and-human-umbilical-vein-endothelial-cells
#19
Xiaojing Guo, Silong Wei, Mengmeng Lu, Zhengwei Shao, Jiayu Lu, Lunguo Xia, Kaili Lin, Derong Zou
In clinic, strontium ranelate (SrR) is a useful drug to treat osteoporosis by orally taken method, but some side effect appeared in recent years. The aim of this study is to evaluate the effectiveness and safety of SrR on cells by direct application, to study the possibility of local application of this drug. Qualitative ALP staining, quantitative ALP activity assay, alizarin red staining, realtime PCR and westernblot assay were used to evaluate the osteogenesis ability of SrR under normal or osteogenic induction environment of ovariectomy bone marrow mesenchymal stem cells (OVX-BMSCs)...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27994514/the-correlation-between-parp1-and-brca1-in-ar-positive-triple-negative-breast-cancer
#20
Jiayan Luo, Juan Jin, Fang Yang, Zijia Sun, Wenwen Zhang, Yaqin Shi, Jing Xu, Xiaoxiang Guan
Triple-negative breast cancer (TNBC) lacks estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER-2) expression and thus cannot benefit from conventional hormonal or anti-HER2 targeted therapies. Anti-androgen therapy has shown a certain effect on androgen receptor (AR) positive TNBC. The emerging researches have proved that poly (ADP-ribose) polymerase (PARP) inhibitor is effective in BRCA1-deficient breast cancers. We demonstrated that combination of AR antagonist (bicalutamide) and PARP inhibitor (ABT-888) could inhibit cell viability and induce cell apoptosis significantly whatever in vitro or in vivo setting in AR-positive TNBC...
2016: International Journal of Biological Sciences
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