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Journal of Biomedical Materials Research. Part A

Silvia M Becerra-Bayona, Viviana R Guiza-Arguello, Brooke Russell, Magnus Höök, Mariah S Hahn
Collagen I interactions with integrins α1 and α2 are known to support human mesenchymal stem cell (hMSC) osteogenesis. Nonetheless, elucidating the relative impact of specific integrin interactions has proven challenging, in part due to the complexity of native collagen. In the present work, we employed two collagen-mimetic proteins - Scl2-2 and Scl2-3 - to compare the osteogenic effects of integrin α1 versus α2 signaling. Scl2-2 and Scl2-3 were both derived from Scl2-1, a triple helical protein lacking known cell adhesion, cytokine binding, and matrix metalloproteinase sites...
May 15, 2018: Journal of Biomedical Materials Research. Part A
Kai Wang, Rajendra Narayan Mitra, Min Zheng, Zongchao Han
The major purpose of this article is to evaluate oligochitosan coated cerium oxide nanoparticles (OCCNPs) alginate laden injectable hydrogels and their potential treatment for age-related macular degeneration (AMD). The water soluble OCCNPs were loaded within injectable hydrogels as antioxidative agents. The release of OCCNPs from hydrogel, radical scavenging properties, and biocompatibility were evaluated and calculated in vitro. The effects of OCCNP laden hydrogel downregulating expression of angiogenic proteins and pro-inflammatory cytokines were quantified in human retinal pigment epithlium-19 (ARPE-19) and umbilical endothelium cell lines...
May 12, 2018: Journal of Biomedical Materials Research. Part A
Oliwia Jeznach, Dorota Kołbuk, Paweł Sajkiewicz
Cartilage loss due to age-related degeneration and mechanical trauma is a significant and challenging problem in the field of surgical medicine. Unfortunately, cartilage tissue can be characterized by the lack of regenerative ability. Limitations of conventional treatment strategies, such as auto-, allo- and xenografts or implants stimulate an increasing interest in the tissue engineering approach to cartilage repair. This review discusses the application of polymer-based scaffolds, with an emphasis on hydrogels in cartilage tissue engineering...
May 4, 2018: Journal of Biomedical Materials Research. Part A
Mohamed Ibrahim, Julia Schoelermann, Kamal Mustafa, Mihaela R Cimpan
Human exposure to titanium dioxide nanoparticles (nano-TiO2 ) is increasing. An internal source of nano-TiO2 is represented by titanium-based orthopedic and dental implants can release nanoparticles (NPs) upon abrasion. Little is known about how the size of NPs influences their interaction with cytoskeletal protein networks and the functional/homeostatic consequences that might follow at the implant-bone interface with regard to osteoblasts. We investigated the effects of size of anatase nano-TiO2 on SaOS-2 human osteoblast-like cells exposed to clinically relevant concentrations (0...
April 30, 2018: Journal of Biomedical Materials Research. Part A
Mattias Pettersson, Jean Pettersson, Margareta Molin Thorén, Anders Johansson
Inflammation and bone reduction around dental implants are described as peri-implantitis and can be caused by an inflammatory response against bacterial products and toxins. Titanium (Ti) forms aggregates with serum proteins, which activate and cause release of the cytokine interleukin (IL-1β) from human macrophages. It was hypothesized that cobalt (Co) ions can interact in the formation of pro-inflammatory aggregates, formed by titanium. To test this hypothesis, we differentiated THP-1 cells into macrophages and exposed them to Ti ions alone or in combination with Co ions to investigate if IL-1β release and cytotoxicity were affected...
April 30, 2018: Journal of Biomedical Materials Research. Part A
Wei Wu, Xifeng Liu, Zifei Zhou, A Lee Miller, Lichun Lu
Three-dimensional structural scaffolds have played an important role in tissue engineering, especially broad applications in areas such as regenerative medicine. We have developed novel biodegradable porous polypropylene fumarate-co-poly (lactic-co-glycolic acid) (PPF-co-PLGA) scaffolds using thermally induced phase separation (TIPS), and determined the effects of critical parameters such as copolymer concentration (6, 8, and 10 wt%) and the binary solvent ratio of dioxane:water (78/22, 80/20, 82/18 wt/wt%) on the fabrication process...
April 29, 2018: Journal of Biomedical Materials Research. Part A
M Parvizi, A H Petersen, C A F M van Spreuwel-Goossens, S G J M Kluijtmans, M C Harmsen
No abstract text is available yet for this article.
April 26, 2018: Journal of Biomedical Materials Research. Part A
Caasy Thomas-Porch, Jie Li, Fabiana Zanata, Elizabeth C Martin, Nicholas Pashos, Kaylynn Genemaras, J Nicholas Poche, Nicholas P Totaro, Melyssa R Bratton, Dina Gaupp, Trivia Frazier, Xiying Wu, Lydia Masako Ferreira, Weidong Tian, Guangdi Wang, Bruce A Bunnell, Lauren Flynn, Daniel Hayes, Jeffrey M Gimble
Decellularized human adipose tissue has potential clinical utility as a processed biological scaffold for soft tissue cosmesis, grafting and reconstruction. Adipose tissue decellularization has been accomplished using enzymatic-, detergent-, and/or solvent-based methods. To examine the hypothesis that distinct decellularization processes may yield scaffolds with differing compositions, the current study employed mass spectrometry to compare the proteomes of human adipose-derived matrices generated through three independent methods combining enzymatic-, detergent-, and/or solvent-based steps...
April 25, 2018: Journal of Biomedical Materials Research. Part A
Shirin Toosi, Nima Behravan, Javad Behravan
Depending on the duration of healing process, 5-10% of bone fractures may result in either non-union or delayed union. Because non-unions remain a clinically important problem, there is interest in the utilization of tissue engineering strategies to augment bone fracture repair. Three basic biologic elements that are required for bone regeneration include cells, extracellular matrix scaffolds and biological adjuvants for growth, differentiation and angiogenesis. Mesenchymal Stem Cells (MSCs) are capable to differentiate into various types of the cells including chondrocytes, myoblasts, osteoblasts, and adipocytes...
April 24, 2018: Journal of Biomedical Materials Research. Part A
B Ö Malekzadeh, M C Erlandsson, P Tengvall, A Palmquist, M Ransjo, M I Bokarewa, A Westerlund
Osteoporosis is a major cause of age-related fractures. Healing complications in osteoporotic patients are often associated with increased mortality and morbidity. Stimulation of the implant-adjacent bone could be beneficial in terms of the surgical outcome. Over the past decade, numerous investigations have implicated insulin in normal bone growth, and recent studies have described the advantages of administering insulin locally to increase bone formation. Therefore, we hypothesized that insulin-coated titanium implants would increase bone formation in osteoporotic animals...
April 19, 2018: Journal of Biomedical Materials Research. Part A
Austin Hensley, Jess Rames, Victor Casler, Christopher Rood, Joshua Walters, Christopher Fernandez, Sanjitpal Gill, Jeremy Mercuri
Intervertebral disc (IVD) degeneration is a multi-factor process that results in the physical destruction of the nucleus pulposus (NP) and annulus fibrosus (AF). This compromises IVD function and causes significant disability and economic burden. Strategies to replace the entire composite structure of the IVD are limited and most approaches do not recapitulate the heterogenous biochemical composition, micro-architecture or mechanical properties of the native tissue. Our central hypothesis was that donor IVDs which resemble the size and biochemistry of human lumbar IVDs could be successfully decellularized while retaining the tissue's structure and function with the long-term goal of creating a composite scaffold for tissue engineering the human IVD...
April 19, 2018: Journal of Biomedical Materials Research. Part A
Mohammad Mousaei Ghasroldasht, Maryam M Matin, Hossein Kazemi Mehrjerdi, Hojjat Naderi-Meshkin, Ali Moradi, Masoud Rajabioun, Faeze Alipour, Samaneh Ghasemi, Mohammad Zare, Mahdi Mirahmadi, Hamid Reza Bidkhori, Ahmad Reza Bahrami
ECM components include a number of osteoinductive and osteoconductive factors, which are involved in bone fracture healing. In this study, a combination of adipose derived mesenchymal stem cells (Ad-MSCs), cancellous bone graft (CBG), and chitosan hydrogel (CHI) was applied to the in non-union bone fracture and healing effects were evaluated for the first time. After creation of animal models with non-union fracture in rats, they were randomly classified into seven groups. Radiography at 0, 2, 4 and 8 weeks after surgery, indicated the positive effects of Ad-MSCs+CBG+CHI and Ad-MSCs+CBG in treatment of bone fractures as early as 2 weeks after the surgery...
April 19, 2018: Journal of Biomedical Materials Research. Part A
Saeed Zahiri, Elahe Masaeli, Elahe Poorazizi, Mohammad Hossein Nasr-Esfahani
Current studies based on regenerative medicine suggest, decellularized extracellular matrix (DC-ECM) components can regulate cell phenotype. In this regard, it is believed, presence of cartilage extracellular matrix particles in culture condition could produce physical and biochemical supportive cues for chondrogenesis. In this study, DC-ECM nanoparticles with average size of 61.5± 22.4 nm were produced by decellularization and mechanical processing. Homogenous distribution and nano scale size of yield particles were observed by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) microscopy imaging...
April 17, 2018: Journal of Biomedical Materials Research. Part A
Jia Dong, Mei Yu, Yan Zhang, Yin Yin, Weidong Tian
Natural and synthetic materials have been developed to provide a three-dimensional (3-D) scaffold that mimics the extracellular matrix properties for tissue regeneration and reconstruction. On account of the excellent biological and mechanical properties, diverse decellularized tissue materials have been developed as natural scaffolds. Among them, decellularized adipose tissue (DAT) has generated great interest in recent years due to the extensiveness of the source and the eximious potential to enhance the regeneration and repair of damaged tissues...
April 17, 2018: Journal of Biomedical Materials Research. Part A
Jimmy Su, Simon C Satchell, Ramille N Shah, Jason A Wertheim
Hydrogels, highly-hydrated crosslinked polymer networks, closely mimic the microenvironment of native extracellular matrix (ECM) and thus present as ideal platforms for three-dimensional cell culture. Hydrogels derived from tissue- and organ-specific decellularized ECM (dECM) may retain bioactive signaling cues from the native tissue or organ that could in turn modulate cell-material interactions and response. In this study, we demonstrate that porcine kidney dECM can be processed to form hydrogels suitable for cell culture and encapsulation studies...
April 17, 2018: Journal of Biomedical Materials Research. Part A
Amit P Khandhar, Gregory J Wilson, Michael G Kaul, Johannes Salamon, Caroline Jung, Kannan M Krishnan
Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles provide a safer alternative to gadolinium-based contrast agents (GBCAs) in T1-weighted MR imaging. MRI contrast behavior of USPIOs depends on their magnetic properties, which in turn depend on their physicochemical composition. Identifying and tailoring USPIO structural characteristics that influence proton relaxation in MRI is crucial to developing effective gadolinium-free T1 contrast agents. Here we present a systematic empirical evaluation of the relationship between USPIO size and MRI relaxivity (r1 and r2 values)...
April 17, 2018: Journal of Biomedical Materials Research. Part A
T Truong, K S Jones
Capsaicin reduced poly(lactic-co-glycolic) acid (PLGA)-induced fibrosis by promoting IL-10 secretion and suppressing alpha-smooth muscle actin (α-SMA) expression. The lifetime and efficacy of tissue engineering scaffolds are determined by the foreign body response. In this study, we investigated the in vitro and in vivo effects of capsaicin to reduce biomaterial-induced fibrosis. RAW 264.7 cells cultured on PLGA films with capsaicin responded with significant (p<0.05) upregulation in M2 markers arginase-1 and IL-10 and downregulation of M1 markers iNOS and IL-12, demonstrating the potential of capsaicin to reduce PLGA-induced inflammation...
April 17, 2018: Journal of Biomedical Materials Research. Part A
Monica Chana, Jill B Lewis, Ryan Davis, Yolanda Elam, David Hobbs, Petra E Lockwood, John C Wataha, Regina L Messer
Corrosion and release of nickel ions from biomedical alloys are well documented, but little is still known about the effects of released nickel ions on cellular function with recurrent inflammatory challenges. Evidence suggests Ni(II) ions amplify LPS-induced secretion of several pro-inflammatory cytokines from monocytes. Exacerbating the inflammatory response, hyperglycemic conditions also affect monocytic function. This study investigated how Ni(II) and hyperglycemic conditions, both singly and in combination, alter monocyte proliferation, mitochondrial activity, inflammatory responses, and differentiation...
April 16, 2018: Journal of Biomedical Materials Research. Part A
Meghan R Pinezich, Lauren N Russell, Nicholas P Murphy, Kyle J Lampe
Biomaterial drug delivery systems (DDS) can be used to regulate growth factor release and combat the limited intrinsic regeneration capabilities of central nervous system (CNS) tissue following injury and disease. Of particular interest are systems that aid in oligodendrocyte regeneration, as oligodendrocytes generate myelin which surrounds neuronal axons and helps transmit signals throughout the CNS. Oligodendrocyte precursor cells (OPCs) are found in small numbers in the adult CNS, but are unable to effectively differentiate following CNS injury...
April 16, 2018: Journal of Biomedical Materials Research. Part A
Ting Guo, Julia P Ringel, Casey G Lim, Laura G Bracaglia, Maeesha Noshin, Hannah B Baker, Douglas A Powell, John P Fisher
Proper cell-material interactions are critical to remain cell function and thus successful tissue regeneration. Many fabrication processes have been developed to create microenvironments to control cell attachment and organization on a 3D scaffold. However, these approaches often involve heavy engineering and only the surface layer can be patterned. We found that 3D extrusion based printing at high temperature and pressure will result an aligned effect on the polymer molecules, and this molecular arrangement will further induce the cell alignment and different differentiation capacities...
April 16, 2018: Journal of Biomedical Materials Research. Part A
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