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Journal of Biomedical Materials Research. Part A

Jizhen Lu, Katrin Kaestle, Jijun Huang, Qiao Liu, Peng Zhang, Ling Gao, James Gardiner, Helmut Thissen, Huang-Tian Yang
Human embryonic stem cell-derived cardiovascular progenitor cells (hESC-CVPCs) hold great promise for cell-based therapies of heart diseases. However, little is known about their niche microenvironment and in particular the required extracellular matrix (ECM) components. Here we screened combinations of surface-immobilized ECM proteins to identify substrates that support the attachment and survival of hESC-CVPCs. Covalent immobilization of ECM proteins laminin (Lm), fibronectin (Fn), collagen I (CI), collagen III (CIII), and collagen IV (CIV) in multiple combinations and concentrations was achieved by reductive amination on transparent acetaldehyde plasma polymer (AAPP) interlayer coatings...
January 13, 2017: Journal of Biomedical Materials Research. Part A
Kazuki Igeta, Yuta Kuwamura, Naohiro Horiuchi, Kosuke Nozaki, Daichi Shiraishi, Mamoru Aizawa, Kazuaki Hashimoto, Kimihiro Yamashita, Akiko Nagai
Synthetic hydroxyapatite (HAp) is used clinically as a material for bone prostheses owing to its good bone-bonding ability; however, it does not contribute to bone remodeling. Carbonate-substituted hydroxyapatite (CAp) has greater bioresorption capacity than HAp while having similar bone-bonding potential, and is therefore considered as a next promising material for bone prostheses. However, the effects of the CAp instability on inflammatory and immune responses are unknown in detail. Here, we show that the surface layer of CAp is more hydrated than that of HAp and induces changes in the shape and function of macrophage-like cells...
January 13, 2017: Journal of Biomedical Materials Research. Part A
J Ribeiro, A R Caseiro, T Pereira, Pas Armada-da-Silva, I Pires, J Prada, I Amorim, I Leal Reis, S Amado, J D Santos, S Bompasso, S Raimondo, Asp Varejão, S Geuna, A L Luís, A C Maurício
The therapeutic effect of three polyvinyl alcohol (PVA) membranes loaded with electrically conductive materials - carbon nanotubes (PVA-CNTs) and polypyrrole (PVA-PPy) - were tested in vivo for neuro-muscular regeneration after an axonotmesis injury in the rat sciatic nerve. The membranes electrical conductivity measured was 1.5±0.5x10(-6) S/m, 579±0.6x10(-6) S/m, and 1837.5±0.7 x10(-6) S/m, respectively. At week-12, a residual motor and nociceptive deficit were present in all treated groups, but at week-12, a better recovery to normal gait pattern of the PVA-CNTs and PVA-PPy treated groups was observed...
January 12, 2017: Journal of Biomedical Materials Research. Part A
Ting Gong, Boon Chin Heng, Jianguang Xu, Shaoyue Zhu, Changyong Yuan, Edward Chin Man Lo, Chengfei Zhang
Dental stem cells can serve as a potential source of functional endothelial cells (ECs) for tissue engineering applications, but the endothelial-lineage differentiation efficiency is rather low even with growth factors and mechanical stimuli, which greatly limits their clinical applications. This is partly due to the deficiency of standard two-dimensional (2-D) culture systems, which is unable to recapitulate the three-dimensional (3-D) in vivo milieu that is rich in extracellular matrix. Hence we extracted decellularized extracellular matrix from human umbilical vein endothelial cells (HUVECs-DECM) to provide a bioactive substratum conducive to the endothelial differentiation of dental stem cells...
January 11, 2017: Journal of Biomedical Materials Research. Part A
Ju-Suk Nam, Ashish Ranjan Sharma, Supriya Jagga, Dong-Hyun Lee, Garima Sharma, Lich Thi Nguyen, Yeon Hee Lee, Jun-Dong Chang, Chiranjib Chakraborty, Sang-Soo Lee
Periprosthetic osteolysis remains the leading obstacle for total joint replacements. Primarily, it was thought that aseptic loosening is mainly caused by macrophage mediated inflammatory process arising from production of wear debris. The role of osteoclasts and its sequential bone resorption ability has been extensively studied, but little is known about impaired osteogenesis during osteolysis. In the current study, we have tried to delineate the regulatory mechanism of osteogenic signals by Ti particles in osteoprogenitor cells as well its participatory role in wear debris induced osteolysis...
January 11, 2017: Journal of Biomedical Materials Research. Part A
Dandan Shi, Jiangwei Xiao, Ruicai Gu, Gang Wu, Hua Liao
The synthesized short peptide-polymers would provide key functions for tissue regeneration and repair, through enriching bioactive molecules on polymers or releasing these molecules pre-conjugated on the materials. We have developed a degradable PU bearing HSNGLPL peptide, which has affinity binding ability to TGF-β. For deeply understanding spatial release of TGF-β from the PU polymers and its localized bioactivity, QCM and Elisa test were used to verify TGF-β binding capacities in vitro and in vivo. The PU polymers, with or without pre-conjugating of TGF-β, were implanted into gastronomies muscle (GN) of C57BL/6 mice, for addressing TGF-β release from the polymers and its bio-regulating function in vivo...
January 11, 2017: Journal of Biomedical Materials Research. Part A
Souvik Mondal, Anupam Giri, Yunjiao Zhang, Samir Kumar Pal, Wei Zhou, Long-Ping Wen
Hidden effects of nano-materials to induced autophagy, a lysosomal degradative pathway, remain an exciting topic, in the level of material-protein interaction and subsequent cellular signaling features. Here, our studies show that surface modified hausmannite nanoparticles (Mn3 O4 NPs) can uniformly cleave/splice Beclin-1 protein and alter cellular mechanism on the emphasis of tuning autophagy and subsequently promote enhancement of apoptosis. Details investigation of Beclin-1 dependency and its uniform cleavage/splice pattern by surface modified Mn3 O4 NPs, shows tuning of cellular mechanism on emphasis of caspase mediated autophagy tuning...
January 11, 2017: Journal of Biomedical Materials Research. Part A
S A Grant, S E Smith, H Schmidt, F Pfeiffer, K Kuroki, S Sherman, R A White, D A Grant
Acellular human gracilis tendons conjugated with gold nanoparticles (AuNP) and hydroxyapatite nanoparticles (nano-HAp) were used as a graft in an anterior cruciate ligament (ACL) reconstruction rabbit model. The ACLs of 11 New Zealand rabbits were reconstructed using grafts conjugated without nanoparticles, with AuNP only, and with both AuNP and nano-HAp. Semi-quantitative histological scoring of bone tunnel portion of grafts was performed after 14 weeks. Bone tunnels were scored for graft degeneration, graft remodeling, percentage of new host fibrous connective, collateral connection, head-to-head connection, graft collagen fiber organization, new host fibrous connective tissue organization, and graft and interface vascularity...
January 11, 2017: Journal of Biomedical Materials Research. Part A
Caifeng Wang, Sangjin Oh, Hyun Ah Lee, Jieun Kang, Ki-Jae Jeong, Seon Woo Kang, Dae Youn Hwang, Jaebeom Lee
Carbon nanotubes (CNTs), with their unique and outstanding properties, such as strong mechanical strength and high electrical conductivity, have become very popular for the repair of tissues, particularly for those requiring electrical stimuli. Polydimethylsiloxane (PDMS)-based elastomers have been used in a wide range of biomedical applications because of their optical transparency, physiological inertness, blood compatibility, non-toxicity, and gas permeability. In present study, most of artificial nerve guidance conduits (ANGCs) are not transparent...
January 11, 2017: Journal of Biomedical Materials Research. Part A
Hanlei Zhou, Chuangang You, Ronghua Jin, Pan Wu, Qiong Li, Chunmao Han
Wound healing is an inherent response resulting in the restoration of tissue integrity. It is a complex process involving cell migration, proliferation, differentiation, apoptosis, and the synthesis and remodeling of the extracellular matrix (ECM). The dermal tissue is an important component of skin that acts as a connecting link between the epidermis and hypodermis. The appearance of scars and contractures after autologous split-thickness skin transplantation or single epidermis diaphragm transplantation for full skin defects indicates that the dermal tissue plays an important role in skin regeneration...
January 7, 2017: Journal of Biomedical Materials Research. Part A
Mohammad A Chowdhury
The metal-organic-frameworks (MOFs) materials are increasingly gaining attraction to utilise into biomedical applications. MOFs are playing a major role to harnessing dual or multiple modalities in therapeutics and diagnostics. MOFs are mostly devised for particular biomedical application by post-synthetic functionalization or modification using variety of polymers, bio-ligands, and silica coating processes. This articles presents a brief overview of two particular areas of biomedical applications where a broad range of MOFs have been used: 1) variety of drug delivery including intracellular drug delivery systems using the MOFs-based carriers; and, 2) development of MOFs-based contrast agents for magnetic resonance image enhancement...
December 29, 2016: Journal of Biomedical Materials Research. Part A
Ki-Jae Jeong, Younseong Song, Hye-Ri Shin, Ji Eun Kim, Jeonghyo Kim, Fangfang Sun, Dae-Youn Hwang, Jaebeom Lee
Chitosan, produced from chitin, is one of the polymers with promising applications in various fields. However, despite diverse research studies conducted on its biocompatibility, its uses are still limited. The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property. In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses...
December 29, 2016: Journal of Biomedical Materials Research. Part A
Elanur Aydın, Hasan Türkez, Fazıl Hacımüftüoğlu, Abdulgani Tatar, Fatime Geyikoğlu
Titanium nanoparticles (NPs) have very wide application areas such as paint, cosmetics, pharmaceuticals and biomedical applications. And, to translate these nanomaterials to the clinic and industrial domains, their safety needs to be verified, particularly in terms of genotoxicity and cytotoxicity. Therefore, in this study, we aimed to investigate of cytotoxicity and changes in gene expression profiles influenced by commonly titanium (as titanium carbide, titanium carbo-nitride, titanium (II) oxide, titanium (III) oxide, titanium (IV) oxide, titanium nitride, titanium silicon oxide) NPs in human alveolar epithelial (HPAEpiC) and pharynx (HPPC) cell lines in vitro since inhalation is an important pathway for exposure to these nanoparticles...
December 28, 2016: Journal of Biomedical Materials Research. Part A
Ahmed El-Hussein, Samuel S K Lam, Joseph Raker, Wei R Chen, Michael R Hamblin
Immunotherapy has become one of the fastest growing areas of cancer research. A promising in situ autologous cancer vaccine (inCVAX) uses a novel immune activator, N-dihydrogalactochitosan (GC), that possesses the ability to stimulate dendritic cells (DC). inCVAX is a combination treatment procedure involving treatment of the tumor with a thermal near-infrared laser to liberate whole cell tumor antigens, followed by injection of GC (a glucosamine polymer with galactose attached to the amino groups) into the treated tumor thereby inducing a systemic anti-tumor immune response...
December 28, 2016: Journal of Biomedical Materials Research. Part A
Corina White, Tyler DiStefano, Ronke Olabisi
Although transplantation of retinal pigment epithelial (RPE) cells has shown promise for the treatment of retinal degenerative diseases, this therapeutic approach is not without challenges. Two major challenges are RPE cell dedifferentiation and inflammatory response following transplantation. The aim of this work is to understand how the rigidity of a scaffold, a relatively unexplored design aspect in retinal tissue engineering, affects RPE cells, particularly the pathways associated with the aforementioned challenges...
December 28, 2016: Journal of Biomedical Materials Research. Part A
Anne Handrini Dewi, Ika Dewi Ana, John Jansen
Calcium sulfate (CS), also known as POP (Plaster of Paris) is a self-setting, biocompatible and osteoconductive biomaterial with a long history for the treatment of skeletal defects. However, calcium sulfate cements show a too fast resorption rate and are unable to provide a long term 3D framework during the osteogenesis process. In our previous studies, it was found that the incorporation of CaCO3 and CaCO3 hydrogel provided enhanced mechanical and degradation properties of POP composites. Furthermore, it was also found that the use of cinnamaldehyde to crosslink CaCO3 hydrogel may have some advantages, e...
December 28, 2016: Journal of Biomedical Materials Research. Part A
Xiao-Li Li, Jun Zhang, Hua-Nan Luo, Xiaoyan Zhao, A-Ling Zhang, Zheng-Hui Wang
The source of seed cells is a critical factor for tissue engineering . The goal of this study was to evaluate the chondrogenesis of Sox9-overexpressing human umbilical cord mesenchymal stem cells (hUCMSCs) seeded onto bone matrix gelatin (BMG)/fibrin hybrid scaffolds both in vitro and in vivo. hUCMSCs were stably transfected with Sox9-expressing plasmid and grown on the three-dimensional BMG/fibrin hybrid scaffold for 8 weeks. Scanning electron microscopy and histochemistry were performed. The hUCMSC-loaded scaffolds were implanted into the subcutaneous layer of immunocompetent rats and chondrogenesis and host immune responses were monitored for 8 weeks...
December 28, 2016: Journal of Biomedical Materials Research. Part A
M Walkowiak-Przybyło, P Komorowski, B Walkowiak
Three medically used alloys (Ti6Al4V, Ti6Al7Nb and AISI 316L) are compared due to proliferative potential and metabolic response of human cells (osteoblasts line Saos-2 and endothelial cells line EA.hy-926) seeded on the surfaces of these alloys. Although no statistically significant difference in the proliferative potential of the cells cultured on the surfaces of examined biomaterials was observed, it does not exclude relevant differences in metabolic response of these cells assessed as changes in genes' expression...
December 21, 2016: Journal of Biomedical Materials Research. Part A
E Jacobs, G Gronowicz, M Hurley, L T Kuhn
Combinations of growth factors synergistically enhance tissue regeneration, but typically require sequential, rather than co-delivery from biomaterials for maximum efficacy. Polyelectrolyte multilayer (PEM) coatings can deliver multiple factors without loss of activity; however, sequential delivery from PEM has been limited due to interlayer diffusion that results in co-delivery of the factors. This study shows that addition of a biomimetic calcium phosphate (bCaP) barrier layer to a PEM coating effectively prevents interlayer diffusion and enables sequential delivery of two different biomolecules via direct cell access...
December 21, 2016: Journal of Biomedical Materials Research. Part A
Ryan M Bock, Erin N Jones, Darin A Ray, B Sonny Bal, Giuseppe Pezzotti, Bryan J McEntire
Perioperative and latent infections are leading causes of revision surgery for orthopaedic devices resulting in significant increased patient care, comorbidities, and attendant costs. Identifying biomaterial surfaces that inherently resist biofilm adhesion and bacterial expression is an important emerging strategy in addressing implant-related infections. This in vitro study was designed to compare biofilm formation on three biomaterials commonly employed in spinal fusion surgery - silicon nitride (Si3 N4 ), polyetheretherketone (PEEK), and a titanium alloy (Ti6Al4V-ELI) - using one gram-positive and one gram-negative bacterial species...
December 21, 2016: Journal of Biomedical Materials Research. Part A
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