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Journal of Biomedical Materials Research. Part A

Raquel C Barros, Edith Gelens, Erna Bulten, Annemarie Tuin, Menno R de Jong, Roel Kuijer, Theo G van Kooten
Nanofibers are thought to enhance cell adhesion, growth, and function. We demonstrate that the choice of building blocks in self-assembling nanofiber systems can be used to control cell behavior. The use of 2 D-coated, self-assembled nanofibers in controlling lens epithelial cells, fibroblasts, and mesenchymal stem cells was investigated, focusing on gene and protein expression related to the fibrotic response. To this end, three nanofibers with different characteristics (morphology, topography, and wettability) were compared with two standard materials frequently used in culturing cells, TCPS, and a collagen type I coating...
May 17, 2017: Journal of Biomedical Materials Research. Part A
Ines Fasolino, Vincenzo Guarino, Valentina Cirillo, Luigi Ambrosio
Incomplete regeneration after trauma or muscular dysfunction is a common problem in muscle replacement therapies. Recent approaches in tissue engineering allow for the replication of skeletal muscle structure and function in vitro and in vivo by molecular therapies and implantable scaffolds which properly address muscle cells towards myotube differentiation and maturation. Here, we investigate the in vitro response of human Mesenchymal Stem Cells (hMSC) on electrospun fibres made of Polycaprolactone (PCL) in the presence of 5-azacytidine (5-AZA) to evaluate how fibrous network may influence the therapeutic effect of drug during in vitro myogenesis...
May 16, 2017: Journal of Biomedical Materials Research. Part A
Dustin L Williams, R Tyler Epperson, Jeffery P DeGrauw, Mattias B Nielsen, Nicholas B Taylor, Ryan D Jolley
Orthodontic retention has been proposed as a life-long commitment for patients who desire to maintain straight teeth. However, the presence of foreign material increases risk of bacterial colonization and caries formation, of which Streptococcus mutans is a key contributor. Multiple studies have assessed the ability of silver to be added to base plate material and resist attachment of S. mutans. However, it does not appear that long term washout in connection with biofilm growth under physiologically relevant conditions has been taken into consideration...
May 16, 2017: Journal of Biomedical Materials Research. Part A
Govinda Bhattarai, Sher Bahadur Poudel, Sung-Ho Kook, Jeong-Chae Lee
Genistein, a dietary polyphenol primarily found in soy products, has beneficial effects on bone. However, the effect of genistein on inflammatory periodontal destruction has not been investigated in detail. We explored whether genistein protects against lipopolysaccharide (LPS)/ligature-induced periodontitis in mice. We also examined the effect of genistein on LPS-stimulated inflammatory and oxidative stress using RAW 264.7 macrophages and human gingival fibroblasts (hGFs). The results from μCT and histological analyses revealed that intraperitoneal injection of genistein (20 mg/kg body weight) daily for three weeks inhibited LPS-mediated alveolar bone loss and periodontal tissue degradation...
May 16, 2017: Journal of Biomedical Materials Research. Part A
M McLuckie, C A Schmidt, A Oosthuysen, N Sanchez-Macedo, H Merker, D Bezuidenhout, S P Hoerstrup, N Lindenblatt
Three-dimensional scaffolds have the capacity to serve as an architectural framework to guide and promote tissue regeneration. Parameters such as the type of material, growth factors, and pore dimensions are therefore critical in the scaffold's success. In this study, heparin has been covalently bound to the surface of macroporous polyurethane (PU) discs via two different loading methods in order to determine if the amount of heparin content had an influence on the therapeutic affinity loading and release of (VEGF165 ) in full thickness skin defects...
May 16, 2017: Journal of Biomedical Materials Research. Part A
Xiaolin Zhang, Oliver W Stockhammer, Leonie de Boer, Norbert O E Vischer, Herman P Spaink, Dirk W Grijpma, Sebastian A J Zaat
In order to rapidly assess early inflammatory cell responses provoked by biomaterials in the full complexity of the living organism, we developed a zebrafish embryo model which allows real time analysis of these responses to biomaterial microspheres. Fluorescently labeled microspheres with different properties were injected into embryos of selected transgenic zebrafish lines expressing distinct fluorescent proteins in their neutrophils and macrophages. Recruitment of leukocytes and their interactions with microspheres were monitored using fluorescence microscopy...
May 16, 2017: Journal of Biomedical Materials Research. Part A
F Kianoush, M Nematollahi, J D Waterfield, D M Brunette
Recognition of topographical features induces phenotypic changes in macrophages although the receptors and signaling pathways are not completely characterized. As integrin molecules in focal adhesions/podosomes are in intimate contact with topography and topography modulates the NFkB pathway through cholesterol enriched raft-associated adhesive signaling structures we hypothesized that a cell-surface signaling complex comprised of galectin-3 together with its ligand CD98 and integrinβ1 is important for topography-directed lineage determination...
May 12, 2017: Journal of Biomedical Materials Research. Part A
M N Biao, Y M Chen, S B Xiong, B Y Wu, B C Yang
In order to improve the biological properties of bioactive titanium metal, recombinant human bone morphogenetic protein 2(rhBMP-2) and fibronectin (Fn) were adsorbed on its surface solely or contiguously to modify the anodic oxidized titanium (AO-Ti), acid-alkali treated titanium (AA-Ti) and polished titanium (P-Ti). It's found the different bioactive titanium surface structures had great influence on protein adsorption. The adsorption amounts of BMP adsorbed solely and Fn/BMP adsorbed contiguously were AA-Ti > P-Ti > AO-Ti, and that for Fn adsorbed solely was AA-Ti≈P-Ti>AO-Ti...
May 12, 2017: Journal of Biomedical Materials Research. Part A
Duc H T Le, Yoko Tsutsui, Ayae Sugawara-Narutaki, Hiroshi Yukawa, Yoshinobu Baba, Chikara Ohtsuki
We have recently developed a novel double-hydrophobic elastin-like triblock polypeptide called GPG, designed after the uneven distribution of two different hydrophobic domains found in elastin, an extracellular matrix protein providing elasticity and resilience to tissues. Upon temperature trigger, GPG undergoes a sequential self-assembling process to form flexible beaded nanofibers with high homogeneity and excellent dispersibility in water. Given that GPG might be a potential elastin-mimetic material, we sought to explore the biological activities of this block polypeptide...
May 9, 2017: Journal of Biomedical Materials Research. Part A
Samaneh Mirzaei, Akbar Karkhaneh, Masoud Soleimani, Abdolreza Ardeshirylajimi, Hasti Seyyed Zonouzi, Hana Hanaee-Ahvaz
Recently, tissue engineering has become one of the most important approaches in medical research for the treatment of injuries and lesions. In the present study, Poly (L-lactide) acid (PLLA), and Poly ethylene glycol (PEG) with different ratios and PEG molecular weights were used in order to produce appropriate nanofibrous scaffolds using the electrospinning technique for cartilage tissue engineering applications. Glucosamine was also incorporated into the polymeric scaffolds to enhance the biological properties...
May 8, 2017: Journal of Biomedical Materials Research. Part A
Chu Gong, Meng Shan, Bingqiang Li, Guolin Wu
An injectable dual redox responsive diselenide-containing polyethylene glycol hydrogel was successfully developed by combing the conceptions of injectable hydrogels and dual redox responsive diselenides. In the first step, 4-armed poly(ethylene glycol) was modified with N-hydroxysuccinimide activated esters and thereafter, cross-linked by selenocystamine cross-linkers to form injectable hydrogels via the rapid reaction between N-hydroxysuccinimide activated esters and amino groups. The cross-sectional morphology, mechanical properties and cross-linking modes of hydrogels were well characterized via scanning electron microscope (SEM), rheological measurements and Fourier transform infrared spectra, respectively...
May 8, 2017: Journal of Biomedical Materials Research. Part A
Alexandra M Smink, Li Shiri, Daniël H Swart, Don T Hertsig, Bart J de Haan, Jan A A M Kamps, Leendert Schwab, Aart A van Apeldoorn, Eelco de Koning, Marijke M Faas, Jonathan R T Lakey, Paul de Vos
The liver as transplantation site for pancreatic islets is associated with significant loss of islets, which can be prevented by grafting in a prevascularized, subcutaneous scaffold. Supporting vascularization of a scaffold to limit the period of ischemia is challenging and was developed here by applying liposomes for controlled release of angiogenic factors. The angiogenic capacity of platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), acidic fibroblast growth factor (aFGF), and basic FGF (bFGF) were compared in a tube formation assay...
May 3, 2017: Journal of Biomedical Materials Research. Part A
Madhura P Nijsure, Meet Pastakia, Joseph Spano, Michael B Fenn, Vipuil Kishore
Bone tissue engineering mandates the development of a functional scaffold that mimics the physicochemical properties of native bone. Bioglass 45S5 (BG) is a highly bioactive material known to augment bone formation and restoration. Hybrid scaffolds fabricated using collagen type I and BG resemble the organic and inorganic composition of the bone extracellular matrix and hence have been extensively investigated for bone tissue engineering applications. However, collagen-BG scaffolds developed thus far do not recapitulate the aligned collagen structure found in native bone...
May 3, 2017: Journal of Biomedical Materials Research. Part A
Christopher R Anderson, Filippo Gambinossi, Katarina M DiLillo, André Laschewsky, Erik Wischerhoff, James K Ferri, Lauren S Sefcik
Thermoresponsive polymer (TRP) cell culture substrates are widely utilized for non-enzymatic, temperature triggered release of adherent cells. Increasingly, multicomponent TRPs are being developed to facilitate refined control of cell adhesion and detachment, which requires an understanding of the relationships between composition-dependent substrate physico-chemical properties and cellular responses. Here, we utilize a homologous series of poly(MEO2 MAx -co-OEGMAy ) brushes with variable copolymer ratio (x/y) to explore the effects of substrate hydrophobicity on L-929 fibroblast adhesion, morphology, and temperature triggered cell detachment...
May 3, 2017: Journal of Biomedical Materials Research. Part A
Daniele Pezzoli, Elisa Cauli, Pascale Chevallier, Silvia Farè, Diego Mantovani
Electrospun PET (ePET) is a promising material for small caliber vascular graft applications owing to its tunable mechanical properties, biocompatibility and nanofibrous structure that mimics the morphology of natural extracellular matrix. However, the inherent inertness of PET impairs the adhesion and proliferation of endothelial cells on the inner surface of ePET tubular grafts, hindering the formation of a functional endothelium. Gelatin coatings, owing to their ability to promote endothelialization, are a valuable approach to overcome the limitations of ePET...
May 3, 2017: Journal of Biomedical Materials Research. Part A
Sujan Lamichhane, Jordan A Anderson, Thomas Vierhout, Tyler Remund, Hongli Sun, Patrick Kelly
Polytetrafluoroethylene (PTFE) is one of the commonly used materials in making various cardiovascular implants. However, the success rates of these implants in several occasions is hindered by unwanted immune responses from immune cells, such as macrophages. In this study, we investigated the response of macrophages with different structures (flat, expanded, and electrospun) of PTFE having varied surface topographies: smooth planar surface (flat PTFE), node-fibrils (ePTFE), and randomly oriented micro fibers (electrospun PTFE)...
May 3, 2017: Journal of Biomedical Materials Research. Part A
Abshar Hasan, Gyan Waibhaw, Sakshi Tiwari, K Dharmalingam, I Shukla, Lalit M Pandey
This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application. CNWs were prepared by acid hydrolysis of cellulose with sulfuric acid. Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length. X-ray powder diffraction (XRD) analysis confirmed highly crystalline nature of CNWs with 92.81% crystallinity. Incorporation of CNWs enhanced the thermal and mechanical properties of films...
April 26, 2017: Journal of Biomedical Materials Research. Part A
Kuihua Zhan, Lun Bai, Qinqin Wu, Derong Lei, Guangqian Wang
The neovascularization of biomaterials for tissue engineering is not only related to growth of capillaries but also involves appropriate hierarchy distribution of the microvessels. In this study, we proposed hierarchy distribution contrast method which can assess vascular transport capacity, in order to examine the hierarchy distribution of the neovessels during vascularization of the porous silk fibroin biomaterials implanted into rats and its evolution. The results showed that the fractal characteristics appeared toward the end of the vascularization stages, and the structure of the microvascular network after 3 weeks of implantation was similar to the fractal microvascular tree with bifurcation exponent x=3 and fractal dimension D=1...
April 26, 2017: Journal of Biomedical Materials Research. Part A
Leyla Saeednia, Li Yao, Marcus Berndt, Kim Cluff, Ramazan Asmatulu
Chitosan has the ability to make injectable thermosensitive hydrogels which has been highly investigated for drug delivery applications. The addition of nanoparticles is one way to increase the mechanical strength of thermosensitive chitosan hydrogel and subsequently and control the burst release of drug. Graphene nanoparticles have shown unique mechanical, optical and electrical properties which can be exploited for biomedical applications, especially in drug delivery. This study, have focused on the mechanical properties of a thermosensitive and injectable hybrid chitosan hydrogel incorporated with graphene nanoparticles...
April 26, 2017: Journal of Biomedical Materials Research. Part A
Le B Hang Pham, Yie-Ri Yoo, Sun Hwa Park, Sang A Back, Sung Won Kim, Isabel Bjørge, Jun-Hyeog Jang
Many extracellular matrix proteins have positive influences on the adhesion, proliferation and differentiation of stem cells into specific cell linages. Fibulin-1 (FBLN1), a member of a growing family of extracellular glycoproteins, contributes to the structure of the extracellular matrix. Here, we investigated the effect of FBLN1 on the ability of human nasal inferior turbinate-derived mesenchymal stem cells (hTMSCs) to undergo osteogenic differentiation. After we generated recombinant FBLN1, the characteristics of FBLN1-treated hTMSCs were evaluated using MTT assay, ALP and mineralization activities, and quantitative real-time PCR...
April 26, 2017: Journal of Biomedical Materials Research. Part A
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