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Journal of Biomedical Materials Research. Part A

Yanan Jiang, Bi Wang, Zhanrong Jia, Xiong Lu, Liming Fang, Kefeng Wang, Fuzeng Ren
Magnesium alloys have the great potential to be used as orthopaedic implants due to their biodegradability and mechanical resemblance to human cortical bone. However, the rapid degradation in physiological environment with the evolution of hydrogen gas release hinders their clinical applications. In this study, we developed a novel functional and biocompatible coating strategy through polydopamine mediated assembly of hydroxyapatite nanoparticles and growth factor, bone morphogenetic protein-2 (BMP-2), onto the surface of AZ31 Mg alloys...
June 12, 2017: Journal of Biomedical Materials Research. Part A
Benjamin D M Coverdale, Julie E Gough, William W Sampson, Judith A Hoyland
We elucidate the effects of incorporating surfactants into electrospun poly (ε-caprolactone) (PCL) scaffolds on network homogeneity, cellular adherence and osteogenic differentiation. Lecithin was added with a range of concentrations to PCL solutions, which were electrospun to yield functionalised scaffolds. Addition of lecithin yielded a dose-dependent reduction in scaffold hydrophobicity, whilst reducing fibre width and hence increasing specific surface area. These changes in scaffold morphology were associated with increased cellular attachment of Saos-2 osteoblasts 3-hours post-seeding...
June 12, 2017: Journal of Biomedical Materials Research. Part A
Ying-Zheng Zhao, Xi Jiang, Qian Lin, He-Lin Xu, Ya-Dong Huang, Cui-Tao Lu, Jun Cai
Traumatic spinal cord injury (SCI) results in paraplegia or quadriplegia, and currently, therapeutic interventions for axonal regeneration after SCI are not clinically available. Animal studies have revealed that glial cell-derived neurotrophic factor (GDNF) plays multiple beneficial roles in neuroprotection, glial scarring remodelling, axon regeneration and remyelination in SCI. However, the poor physicochemical stability of GDNF, as well as its limited ability to cross the blood-spinal cord barrier, hampers the development of GDNF as an effective therapeutic intervention in clinical practice...
June 7, 2017: Journal of Biomedical Materials Research. Part A
Sharon L Hyzy, Illya Kajan, D Scott Wilson, Kelsey A Lawrence, Devon Mason, Joseph K Williams, Rene Olivares-Navarrete, David J Cohen, Zvi Schwartz, Barbara D Boyan
Biologics can improve bone formation, but may diffuse away from sites of therapeutic need. We developed a click-chemistry hydrogel that rapidly polymerizes in situ to control delivery of biologics during post-suturectomy resynostosis in 21-d-old male mice. Here, we used this model to determine the role of angiogenesis in post-suturectomy resynostosis and examine whether controlled release of angiogenesis inhibitors could delay bone regeneration. Hydrogels [DB-co-PEG/poly(TEGDMA)-co-(N3-TEGDMA)] were produced containing anti-angiogenic compounds (anti-VEGFA-antibody or HIF1α-inhibitor topotecan)...
June 7, 2017: Journal of Biomedical Materials Research. Part A
Shima Tanha, Morteza Rafiee-Tehrani, Mohamad Abdollahi, Saeid Vakilian, Zahra Esmaili, Zahra Safaei Naraghi, Ehsan Seyedjafari, Hamid Akbari Javar
Sustained release of functional growth factors can be considered as a beneficial methodology for wound healing. In this study, recombinant human granulocyte colony-stimulating factor (G-CSF)-loaded chitosan nanoparticles were incorporated in PCL nanofibers, followed by surface coating with collagen type I. Physical and mechanical properties of the PCL nanofibers containing G-CSF loaded chitosan nanoparticles PCL/NP(G-CSF) and in vivo performance for wound healing were investigated. G-CSF structural stability was evaluated through SDS_PAGE, reversed phase (RP)HPLC and size-exclusion chromatography (SEC), as well as circular dichroism (CD)...
June 7, 2017: Journal of Biomedical Materials Research. Part A
E V Sokolova, A S Kuz'mich, A O Byankina, L M Yermak
The research described here was focused on the effect on human intestinal epithelial cell monolayers of sulphated red algal polysaccharides (κ-, λ-, and κ/β-carrageenans) alone and in combination with casein or lipopolysaccharide (LPS). HT-29 cells were investigated under normal and stress conditions; stress was induced by exposure to ethanol. Cell viability was monitored with a real-time system. The change in binding properties of negatively sulphated red algal polysaccharides assessed by the measurement of free carrageenans in mixtures with casein or McCoy's 5A culture medium by means of toluidine blue O...
June 7, 2017: Journal of Biomedical Materials Research. Part A
Cristina Velasquillo, Phaedra Silva-Bermudez, Nadia Vázquez, Alan Martínez, Andres Espadín, Julieta García-López, Antonio Medina-Vega, Hugo Lecona, Raúl Pichardo, Clemente Ibarra, Keiko Shirai
Autologous skin transplantation is today₹s "gold standard" treatment for full-thickness burns. However, when more than 30% of total body surface area is damaged, there is an important shortage of autologous donor sites for skin grafting; then, treatment alternatives become crucial. Such alternatives can be based on polymeric scaffolds capable of functioning as protective covers and cells/factors carriers. Chitosan (CTS) is a natural-derived polymer with relevant biological-related properties but poor mechanical performance...
June 7, 2017: Journal of Biomedical Materials Research. Part A
Sudip Dahal, Peter Huang, Bruce T Murray, Gretchen J Mahler
Alterations in shear stress, mechanical deformation, extracellular matrix (ECM) composition and exposure to inflammatory conditions are known to cause endothelial to mesenchymal transformation (EndMT). This change in endothelial phenotype has only recently been linked to adult pathologies such as cancer progression, organ fibrosis, and calcific aortic valve disease; and its function in adult physiology, especially in response to tissue mechanics, has not been rigorously investigated. EndMT is a response to mechanical and biochemical signals that results in the remodeling of underlying tissues...
June 7, 2017: Journal of Biomedical Materials Research. Part A
Taimoor H Qazi, Shahzad Hafeez, Jochen Schmidt, Georg N Duda, Aldo R Boccaccini, Evi Lippens
Bioactive glasses (BAGs) are highly interesting materials for bone regeneration applications in orthopedic and dental defects. It is quite well known that ionic release from BAGs influences cell behavior and function. Mindful of the clinical scenario, we hypothesized that local cell populations might additionally physically interact with the implanted BAG particles and respond differently than to just the ionic stimuli. We therefore studied the biological effect of two BAG types (45S5 and 1393) applied to human mesenchymal stromal cells (hMSCs) in three distinct presentation modes: (a) direct contact; and to dissolution products in (b) 2D, and (c) 3D culture...
June 1, 2017: Journal of Biomedical Materials Research. Part A
Hadi Shirali, Mehdi Rafizadeh, Faramarz Afshar Taromi, Esmaiel Jabbari
A combination of elastic poly(butylene succinate-co-ethylene terephthalate) and rigid nano-hydroxyapatite were used to prepare an in-situ synthesized nanocomposite mimicing bone structure. The microstructure, morphology and dispersion of nanoparticles in the nanocomposites were studied using (1) H-NMR, SEM and TEM, respectively. Then, electrospinning method was used to produce nanofiber matrix with lowest fiber diameter. Presence of chemical bonds among nanoparticles and polymer leads to the excellent particle dispersion and solution phase stability...
June 1, 2017: Journal of Biomedical Materials Research. Part A
Muhammad Shafiq, Deling Kong, Soo Hyun Kim
The design of bioactive scaffolds that can invoke host's own regenerative capabilities and facilitate endogenous tissue repair hold great promise. This study aims to evaluate the potential of stromal cell-derived factor 1 alpha (SDF-1α)-derived peptide and heparin tethered poly(L-lactide-co-ε-caprolactone) (PLCL) copolymers for blood vessel regeneration applications. Amino acid analysis and toluidine blue assays confirm successful conjugation of SDF-1α peptide and heparin with the PLCL copolymers. Assessment of biocompatibility after subcutaneous implantation in rats discloses higher cell infiltration in SDF-1α peptide (SDF-1 group) or SDF-1 peptide and heparin (SDF-1/heparin group) than the control group...
June 1, 2017: Journal of Biomedical Materials Research. Part A
Hadar Zigdon-Giladi, Alaa Khutaba, Rina Elimelech, Eli E Machtei, Samer Srouji
Angiogenesis plays a pivotal role in tissue engineering and regenerative medicine. This study aimed to develop an electrospun fiber scaffold that supports release of recombinant human vascular endothelial growth factor (rhVEGF) to enhance angiogenesis. Scaffolds composed of core-shell fibers were fabricated using co-electrospinning. The core solution was composed of polyethylene oxide and mixed with rhVEGF. The shell solution was composed of polycarpolactone, with 0.25, 1, and 3% of polyethylene glycol (PEG) to manipulate pore size on the shell...
May 27, 2017: Journal of Biomedical Materials Research. Part A
Junxi Xiang, Peng Liu, Xinglong Zheng, Dinghui Dong, Shujuan Fan, Jian Dong, Xufeng Zhang, Xuemin Liu, Bo Wang, Yi Lv
Weak mechanical property and unstable degradation rate limited the application of decellularized liver matrix in tissue engineering. The aim of this study was to explore a new method for improving the mechanical properties, anti-degeneration and angiogenic capability of decellularized liver matrix. This was achieved by a novel approach using riboflavin/ultraviolet A treatment to induce collagen cross-linking of decellularized matrix. Histological staining and scanning electron microscope showed that the diameter of cross-linked fibers significantly increased compared with the control group...
May 27, 2017: Journal of Biomedical Materials Research. Part A
Yashdeep Phanse, Brenda R Carrillo-Conde, Amanda E Ramer-Tait, Rajarshi Roychoudhury, Scott Broderick, Nicola Pohl, Krishna Rajan, Balaji Narasimhan, Michael J Wannemuehler, Bryan H Bellaire
Rational design of adjuvants and delivery systems will promote development of next-generation vaccines to control emerging and re-emerging diseases. To accomplish this, understanding the immune enhancing properties of new adjuvants relative to those induced by natural infections can help with the development of pathogen mimicking materials that will effectively initiate innate immune signaling cascades. In this work, the surfaces of polyanhydride nanoparticles composed of sebacic acid (SA) and 1,6-bis(p-carboxyphenoxy) hexane (CPH) were decorated with an ethylene diamine spacer partially modified with either a glycolic acid linker or an α-1,2-linked di-mannopyranoside (di-mannose) to confer "pathogen-like" properties and enhance adjuvanticity...
May 27, 2017: Journal of Biomedical Materials Research. Part A
Fatemeh Soleimanifar, Yousef Mortazavi, Samad Nadri, Masoud Soleimani
With the aid of tissue engineering, the capability of nanofibrous scaffolds was examined for corneal epithelial differentiation of Conjunctiva mesenchymal stem cells (CJMSCs) as a new source. This study tried to provide a synthetic corneal sheet which could be applied in corneal regeneration. The hybrid arrangement of fine Silk fibers with the diameter of 93.65 nm and the polyurethane (PU) fibers with an average diameter of 575.5 makes a mat with sufficient mechanical properties and interconnected pores that could accelerate nutrient diffusion...
May 27, 2017: Journal of Biomedical Materials Research. Part A
Alysha P Kishan, Elizabeth M Cosgriff-Hernandez
Electrospinning, a technique used to fabricate fibrous scaffolds, has gained popularity in recent years as a method to produce tissue engineered grafts with architectural similarities to the extracellular matrix. Beyond its versatility in material selection, electrospinning also provides many tools to tune the fiber morphology and scaffold geometry. Recent efforts have focused on extending the capabilities of electrospinning to produce scaffolds that better recapitulate tissue properties and enhance regeneration...
May 27, 2017: Journal of Biomedical Materials Research. Part A
Masako Fujioka-Kobayashi, Eizaburo Kobayashi, Benoit Schaller, Matthias Mottini, Richard J Miron, Nikola Saulacic
Recent research has demonstrated that recombinant human bone morphogenetic protein 9 (rhBMP9) has been considered the most osteoinductive growth factor of the BMP-family. In the present study, rhBMP9 was investigated for its influence in combination with two biomaterials for bone regenerative medicine. Either porcine-derived collagen membrane (CM) or deproteinized bovine bone mineral (DBM) combined with 20 µg of rhBMP9 were implanted in 6 mm rabbit calvarial defects. Bone augmentation was evaluated by microCT and histomorphometry at 8 weeks post-surgery...
May 27, 2017: Journal of Biomedical Materials Research. Part A
Kristine M Fischenich, Katie Boncella, Jackson T Lewis, Travis S Bailey, Tammy L Haut Donahue
Understanding how human meniscal tissue responds to loading regimes mimetic of daily life as well as how it compares to larger animal models is critical in the development of a functionally accurate synthetic surrogate. Seven human and 8 ovine cadaveric meniscal specimens were regionally sectioned into cylinders 5mm in diameter and 3 mm thick along with 10 polystyrene-b-polyethylene oxide block copolymer-based thermoplastic elastomer (TPE) hydrogels. Samples were compressed to 12% strain at 1 Hz for 5000 cycles, unloaded for 24 hours, and then retested...
May 27, 2017: Journal of Biomedical Materials Research. Part A
Margaret K Boushell, Nora T Khanarian, Raquel Z LeGeros, Helen H Lu
The osteochondral interface functions as a structural barrier between cartilage and bone, maintaining tissue integrity postinjury and during homeostasis. Regeneration of this calcified cartilage region is thus essential for integrative cartilage healing, and hydrogel-ceramic composite scaffolds have been explored for calcified cartilage formation. The objective of this study is to test the hypothesis that Ca/P ratio of the ceramic phase of the composite scaffold regulates chondrocyte biosynthesis and mineralization potential...
May 25, 2017: Journal of Biomedical Materials Research. Part A
Reza Khorramirouz, Seyedeh Maryam Kameli, Sahar Eftekharzadeh, Abdol-Mohammad Kajbafzadeh
This study investigates the extent of cell seeding as well as lumen patency in acellular Internal mammary artery (IMA) scaffolds that have been re-cellularized by omentum as a natural bioreactor. Sixteen Virgin female Wistar rats were selected for implantation of acellular scaffold in omentum. Following laparotomy omentum was retracted to the outside of the abdomen and the more vascularized portion of it was selected for the experiment. The scaffold was wrapped by omentum and placed between two layers of rectus muscles that have been previously dissected...
May 25, 2017: Journal of Biomedical Materials Research. Part A
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