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Nanomedicine: Nanotechnology, Biology, and Medicine

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https://www.readbyqxmd.com/read/28219741/regulation-of-human-gdnf-gene-expression-in-nigral-dopaminergic-neurons-using-a-new-doxycycline-regulated-nts-polyplex-nanoparticle-system
#1
Armando J Espadas-Alvarez, Michael J Bannon, Carlos E Orozco-Barrios, Lourdes Escobedo-Sanchez, Jose Ayala-Davila, David Reyes-Corona, Guadalupe Soto-Rodriguez, Vicente Escamilla-Rivera, Andrea De Vizcaya-Ruiz, M Eugenia Gutierrez-Castillo, America Padilla-Viveros, Daniel Martinez-Fong
The human glial-cell derived neurotrophic factor (hGDNF) gene transfer by neurotensin (NTS)-polyplex nanoparticles functionally restores the dopamine nigrostriatal system in experimental Parkinson's disease models. However, high levels of sustained expression of GDNF eventually can cause harmful effects. Herein, we report an improved NTS-polyplex nanoparticle system that enables regulation of hGDNF expression within dopaminergic neurons. We constructed NTS-polyplex nanoparticles containing a single bifunctional plasmid that codes for the reverse tetracycline-controlled transactivator advanced (rtTA-Adv) under the control of NBRE3x promoter, and for hGDNF under the control of tetracycline-response element (TRE)...
February 17, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28216150/novel-biodegradable-poly-gamma-glutamic-acid-amphotericin-b-complexes-show-promise-as-improved-amphotericin-b-formulations
#2
T Dinh, Q Zia, S Zubair, P Stapleton, R Singh, M Owais, S Somavarapu
Commercially available amphotericin B (AmB) formulations are limited either by cytotoxicities, lower efficacies, shelf-life related issues or high production costs. AmB complexes based on poly(gamma-glutamic acid) (PGGA) have been prepared and evaluated for their efficacies against AmB-deoxycholate (Fungizone®) and liposomal AmB (AmBisome®). Physical characterizations showed that AmB/PGGA complexes are nanoscopic (20-40nm) with a negative zetapotential (-51.0mV), water-soluble, stable in solution (up to 4weeks, at 4 °C and 25 °C), and have a theoretical drug loading (up to 76...
February 16, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28214610/virosome-bound-antigen-enhances-dc-dependent-specific-cd4-t-cell-stimulation-inducing-a-th1-and-treg-profile-in-vitro
#3
Rebecca A M Blom, Mario Amacker, Christian Moser, R Maarten van Dijk, Raffaela Bonetti, Emilie Seydoux, Sean R R Hall, Christophe von Garnier, Fabian Blank
There is considerable interest to develop antigen-carriers for immune-modulatory clinical applications, but insufficient information is available on their effects on antigen-presenting cells. We employed virosomes coupled to ovalbumin (OVA) to study their interaction with murine bone marrow-derived dendritic cells (BMDCs) and modulation of downstream T cell responses. BMDCs were treated in vitro with virosomes or liposomes prior to determining BMDC phenotype, viability, and intra-cellular trafficking. Antigen-specific CD4(+) T cell activation was measured by co-culture of BMDCs with DO11...
February 15, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28214609/evaluation-of-vascular-tone-and-cardiac-contractility-in-response-to-silver-nanoparticles-using-langendorff-rat-heart-preparation
#4
Ramirez-Lee Manuel Alejandro, Martinez-Cuevas Pedro Pablo, Rosas-Hernandez Hector, Oros-Ovalle Cuauhtémoc, Bravo-Sanchez Mariela, Martinez-Castañon Gabriel Alejandro, Gonzalez Carmen
Silver nanoparticles (AgNPs) have been widely used because of their antimicrobial properties. However, several reports suggest that AgNPs exposure promote cardiac effects that involve nitric oxide (NO) and oxidative stress (OS). Nevertheless, there are no studies related to AgNPs-induced effects in cardiac physiology. The aim of this study was to evaluate the AgNPs direct actions on coronary vascular tone and cardiac contractility using Langendorff rat heart preparation. Low concentrations of AgNPs (0.1 and 1μg/mL) increased NO derived from inducible NO-synthase (iNOS), without modifying cardiac parameters...
February 15, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28214608/near-infrared-activatable-phthalocyanine-poly-l-glutamic-acid-conjugate-increased-cellular-uptake-and-light-dark-toxicity-ratio-towards-an-effective-photodynamic-cancer-therapy
#5
Lik Voon Kiew, Hoay Yan Cheah, Siew Hui Voon, Elena Gallon, Julie Movellan, Kia Hui Ng, Serkan Alpugan, Hong Boon Lee, Fabienne Dumoulin, María J Vicent, Lip Yong Chung
In photodynamic therapy (PDT), the low absorptivity of photosensitizers in an aqueous environment reduces singlet oxygen generation efficiency and thereby decreases photosensitizing efficacy in biological conditions. To circumvent this problem, we designed a phthalocyanine-poly-L-glutamic acid conjugate (1-PG) made from a new phthalocyanine (Pc 1) monofunctionalized to allow adequate conjugation to PGA. The resulting 1-PG conjugate retained high absorptivity in the near-infrared (NIR) region at its λmax 675nm in an aqueous environment...
February 15, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28214607/real-time-liver-uptake-and-biodistribution-of-magnetic-nanoparticles-determined-by-ac-biosusceptometry
#6
Caio C Quini, André G Próspero, Marcos F F Calabresi, Gustavo M Moretto, Nicholas Zufelato, Sunil Krishnan, Diana R Pina, Ricardo B Oliveira, Oswaldo Baffa, Andris F Bakuzis, Jose R A Miranda
We describe the development of a joint in vivo/ex vivo protocol to monitor magnetic nanoparticles in animal models. Alternating current biosusceptometry (ACB) enables the assessment of magnetic nanoparticle accumulation, followed by quantitative analysis of concentrations in organs of interest. We present a study of real-time liver accumulation, followed by the assessment of sequential biodistribution using the same technique. For quantification, we validated our results by comparing all of the data with electron spin resonance (ESR)...
February 15, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28214606/payload-release-by-liposome-burst-thermal-collapse-of-microgels-induces-satellite-destruction
#7
Alexander Yaroslavov, Irina Panova, Andrey Sybachin, Vasiliy Spiridonov, Alexander Zezin, Olga Mergel, Arjan Gelissen, Rahul Tiwari, Felix Plamper, Walter Richtering, Fredric Menger
No abstract text is available yet for this article.
February 15, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28185940/twisting-electrospun-nanofiber-fine-strips-into-functional-sutures-for-sustained-co-delivery-of-gentamicin-and-silver
#8
Shixuan Chen, Liangpeng Ge, Aubrey Mueller, Mark A Carlson, Matthew J Teusink, Franklin D Shuler, Jingwei Xie
Surgical site infections (SSIs) represent the most common nosocomial infection among surgical patients. In order to prevent SSIs in a sustained manner and lessen side effects, we developed a twisting method for generation of nanofiber-based sutures capable of simultaneous delivery of silver and gentamicin. The prepared sutures are composed of core-sheath nanofibers with gentamicin/pluronic F127 in the core and silver/PCL in the sheath produced by co-axial electrospinning. The diameters of obtained sutures range from ~ 80 μm to ~ 1...
February 6, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28185939/ph-redox-and-photothermal-tri-responsive-dna-polyethylenimine-conjugated-gold-nanorod-as-nanocarrier-for-specific-intracellular-co-release-of-doxorubicin-and-chemosensitizer-pyronaridine-to-combat-multidrug-resistant-cancer
#9
Yun Wang, Zhipeng Zhang, Shaohui Xu, Feihu Wang, Yuanyuan Shen, Shengtang Huang, Shengrong Guo
Pharmacotherapy of multidrug resistant (MDR) cancer remains a challenging task in clinic. Herein, a pH-responsive DNA and disulfide-linked polyethylenimine functionalized gold nanorod was developed for specific co-delivery of chemotherapeutic agent doxorubicin (DOX) and chemosensitizer pyronaridine (PND) to effectively overcome MDR cancer cells. DOX and PND were firstly carried by a multifunctional nanocomplex for reversing MDR cancer. The nanocomplex can responsively and rapidly release its drugs payload under acidic pH environment (pH, ~ 5), intracellular GSH concentration content (5mM) and/or 808nm NIR laser irradiation...
February 6, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28185938/folate-targeted-nanoparticle-delivery-of-androgen-receptor-shrna-enhances-the-sensitivity-of-hormone-independent-prostate-cancer-to-radiotherapy
#10
Xinjun Zhang, Nianli Liu, ZhiYing Shao, Hui Qiu, Hong Yao, JiaYin Ji, Jianshe Wang, Wenchao Lu, Ronald C Chen, Longzhen Zhang
Androgen receptor (AR) plays a crucial role in the development and progression of prostate cancer (PCa). PCa patients typically receive androgen deprivation therapy; nonetheless, these patients eventually develop castration and radiation resistance. We hypothesized that we could further improve radiotherapeutic efficacy of hormone-independent PCa (HIPC) by silencing AR. In this study, nanoparticle (NP) AR-shRNA was formulated using folate-targeted H1 nanopolymer. We demonstrated that NP AR-shRNA enhances PCa radiosensitivity as indicated by the inhibition of cell growth, increased apoptosis, and increased cell cycle arrest in AR-dependent HIPC in vitro...
February 6, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28137659/smuggling-gold-nanoparticles-across-cell-types-a-new-role-for-exosomes-in-gene-silencing
#11
Catarina Roma-Rodrigues, Francisca Pereira, António P Alves de Matos, Marta Fernandes, Pedro V Baptista, Alexandra R Fernandes
Once released to the extracellular space, exosomes enable the transfer of proteins, lipids and RNA between different cells, being able to modulate the recipient cells' phenotypes. Members of the Rab small GTP-binding protein family, such as RAB27A, are responsible for the coordination of several steps in vesicle trafficking, including budding, mobility, docking and fusion. The use of gold nanoparticles (AuNPs) for gene silencing is considered a cutting-edge technology. Here, AuNPs were functionalized with thiolated oligonucleotides anti-RAB27A (AuNP@PEG@anti-RAB27A) for selective silencing of the gene with a consequent decrease of exosomes´ release by MCF-7 and MDA-MB-453 cells...
January 27, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28131884/inflammation-induced-brain-endothelial-activation-leads-to-uptake-of-electrostatically-stabilized-iron-oxide-nanoparticles-via-sulfated-glycosaminoglycans
#12
Dominique Berndt, Jason M Millward, Jörg Schnorr, Matthias Taupitz, Verena Stangl, Friedemann Paul, Susanne Wagner, Jens T Wuerfel, Ingolf Sack, Antje Ludwig, Carmen Infante-Duarte
Based on our previous data on the presence of very small superparamagnetic iron oxide nanoparticles (VSOP) on brain endothelial structures during experimental autoimmune encephalomyelitis (EAE), we investigated the mechanisms of VSOP binding on inflamed brain endothelial cells in vivo and in vitro. After intravenous application, VSOP were detected in brain endothelial cells of EAE animals at peak disease and prior to clinical onset. In vitro, inflammatory stimuli increased VSOP uptake by brain endothelial bEnd...
January 25, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28131883/layer-by-layer-self-assembled-multilayers-on-peek-implants-improve-osseointegration-in-an-osteoporosis-rabbit-model
#13
Xilin Liu, Fei Han, Peng Zhao, Chao Lin, Xuejun Wen, Xiaojian Ye
This study aims to fabricate and deposit nanoscale multilayers on polyetheretherketone (PEEK) to improve cell adhesion and osseointegration. Bio-activated PEEK constructs were designed with prepared surface of different layers of polystyrene sulfonate (PSS) and polyallylamine hydrochloride (PAH) multilayers. Irregular morphology was found on the 5 and 10-layer PEEK surfaces, while "island-like" clusters were observed for 20-layer (20 L) multilayers. Besides, the 20 L PEEK showed more hydrophilic feature than native PEEK, and the surface contact angle reduced from 39...
January 25, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28131882/in-vitro-flow-through-assay-for-rapid-detection-of-endotoxin-in-human-sera-a-proof-of-concept
#14
Prasanta Kalita, Lakshmi M Chaturvedula, Venkataraman Sritharan, Shalini Gupta
An increase in endotoxin concentration in the bloodstream can trigger activation of innate immune response leading to septic shock. There is currently no method available for rapid endotoxin detection at a patient's bedside. We demonstrate a simple, portable and cost-effective strategy to measure endotoxin levels in human serum within 5min using a flow-through assay. A drop of serum containing LPS was spotted on an endotoxin-affinity membrane placed over high-wicking absorbent pads. Subsequent addition of polymyxin B sulfate drug-conjugated gold nanoparticles allowed concentration-dependent visualization of spots by the naked eye in the clinically-relevant range of 10pg/mL to 10ng/mL...
January 25, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28115254/design-synthesis-and-evaluation-of-biotin-decorated-inulin-based-polymeric-micelles-as-long-circulating-nanocarriers-for-targeted-drug-delivery
#15
Delia Mandracchia, Antonio Rosato, Adriana Trapani, Theodora Chlapanidas, Isabella Monia Montagner, Sara Perteghella, Cinzia Di Franco, Maria Luisa Torre, Giuseppe Trapani, Giuseppe Tripodo
Here, long-circulating behaviours of Inulin-based nanomicelles are demonstrated for the first time in vivo. We show the synthesis and evaluation of biotin (BIO)-decorated polymeric INVITE micelles constituted of substances of natural origin, Inulin (INU) and Vitamin E (VITE), as long-circulating carriers for receptor-mediated targeted drug delivery. The resulting INVITE or INVITE-BIO micelles, nanometrically sized, did not reveal any cytotoxicity after 24h of incubation with Caco-2 cells. Moreover, in vitro studies on Caco-2 cells monolayers indicated that the transport of INVITE-BIO micelles was faster than surface unmodified INVITE micelles...
January 20, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28115253/gold-nanorods-reflectance-discriminate-benign-from-malignant-oral-lesions
#16
Abraham Hirshberg, Irit Allon, Ilya Novikov, Rinat Ankri, Ariel Ashkenazy, Dror Fixler
Nanoparticle-based contrast agents have been used as an imaging tool for selectively detecting cancerous processes. We aimed to evaluate the detection sensitivity of reflection measurements of gold nanorods (GNRs) bio-conjugated to anti-epidermal growth factor receptor (GNRs-EGFR) monoclonal antibodies in discriminating benign from premalignant and malignant human oral lesions. Tissue sections incubated with GNRs-EGFR and the reflectance spectrum was measured using hyperspectral microscopy. Reflectance intensity increased with the progression of the disease, lowest in the control group and increasing as the dysplastic changes increase (p<0...
January 20, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28115252/micropatterning-of-nanocomposite-polymer-scaffolds-using-sacrificial-phosphate-glass-fibers-for-tendon-tissue-engineering-applications
#17
Feras Alshomer, Camilo Chaves, Tiziano Serra, Ifty Ahmed, Deepak M Kalaskar
This study presents a simple and reproducible method of micropatterning the novel nanocomposite polymer (POSS-PCU) using a sacrificial phosphate glass fiber template for tendon tissue engineering applications. The diameters of the patterned scaffolds produced were dependent on the diameter of the glass fibers (15 μm) used. Scaffolds were tested for their physical properties and reproducibility using various microscopy techniques. For the first time, we show that POSS-PCU supports growth of human tenocytes cells...
January 20, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28115251/spatial-controlled-multistage-nanocarriers-through-hybridization-of-dendrimers-and-gelatin-nanoparticles-for-deep-penetration-and-therapy-into-tumor-tissue
#18
Yuchao Fan, Shengxiao Yuan, MengMeng Huo, Arka Sen Chaudhuri, Meihui Zhao, Zhenghong Wu, Xiaole Qi
Most nanoparticles (NPs) have difficulty deeply penetrating into tumor tissues. Here, we designed a spatially controlled multistage nanocarrier by encapsulating small polyamidoamine (PAMAM) dendrimers (~5nm) within large gelatin NPs (~200nm). This multistage nanocarrier is meant to be stable during systemic circulation and to leak through tumor vasculature walls by the enhanced permeation and retention (EPR) effect. Afterwards, this multistage nanocarrier release PAMAM dendrimers in response to the high matrix metalloproteinase-2 (MMP-2) enzymes in the tumor microenvironment, and further transport into tumor cells...
January 20, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28115250/lead-adsorption-anticoagulation-and-in-vivo-toxicity-studies-on-the-new-magnetic-nanomaterial-fe3o4-sio2-dmsa-as-a-hemoperfusion-adsorbent
#19
Yang Xiang, Zhiming Bai, Shufang Zhang, Yan Sun, Shunlan Wang, Xiaobin Wei, Wenshi Mo, Jiale Long, Zhenxiang Liu, Chao Yang, Linlin Zheng, Xueyi Guo, Wang Xiaoyang, Fangfang Mao, Ningchuan Feng
This project aimed to develop and characterize a new nanoadsorbent for hemoperfusion. Fe3O4 nanoparticles synthesized by a facile solvothermal method were coated with SiO2 and further modified by DMSA. TEM, XRD, FTIR, XPS and SEM were performed before and after lead adsorption to reveal the general performance and adsorption mechanism. Rabbit lead poisoning models were established to study the adsorption rate; then, a pig hemoperfusion experiment was used for further validation. In addition, coagulation, liver, kidney and heart function, blood lipids, electrolytes and the immune inflammatory system were studied before and after hemoperfusion...
January 20, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28115249/rapid-and-high-throughput-colorimetric-screening-for-anti-aggregation-reagents-of-protein-conformational-diseases-by-using-gold-nanoplasmonic-particles
#20
Hye Young Kim, Jung A Kwon, Taewook Kang, Inhee Choi
Cellular deposition of destabilized proteins and their aggregates is considered one of the most indisputable factors implicated in protein conformational diseases. Here, we report an innovative high-throughput screening method for discovering anti-aggregation reagents out of numerous potential candidates by using gold nanoplasmonic particles. In our method, nanoparticles act as catalytic activators to accelerate protein aggregation and simultaneously exhibit a colorimetric response according to their embedded shape on the protein aggregates...
January 20, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
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