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Virology Journal

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https://www.readbyqxmd.com/read/28821267/small-size-recombinant-adenoviral-hexon-protein-fragments-for-the-production-of-virus-type-specific-antibodies
#1
Martin Pacesa, Rodinde Hendrickx, Manuela Bieri, Justin W Flatt, Urs F Greber, Silvio Hemmi
BACKGROUND: Adenoviruses are common pathogens infecting animals and humans. They are classified based on serology, or genome sequence information. These methods have limitations due to lengthy procedures or lack of infectivity data. Adenoviruses are easy to produce and amenable to genetic and biochemical modifications, which makes them a powerful tool for biological studies, and clinical gene-delivery and vaccine applications. Antibodies directed against adenoviral proteins are important diagnostic tools for virus identification in vivo and in vitro, and are used to elucidate infection mechanisms, often in combination with genomic sequencing and type specific information from hyper-variable regions of structural proteins...
August 18, 2017: Virology Journal
https://www.readbyqxmd.com/read/28818113/respiratory-syncytial-virus-rsv-entry-is-inhibited-by-serine-protease-inhibitor-aebsf-when-present-during-an-early-stage-of-infection
#2
Winke Van der Gucht, Annelies Leemans, Marjorie De Schryver, Annick Heykers, Guy Caljon, Louis Maes, Paul Cos, Peter L Delputte
BACKGROUND: Host proteases have been shown to play important roles in many viral activities such as entry, uncoating, viral protein production and disease induction. Therefore, these cellular proteases are putative targets for the development of antivirals that inhibit their activity. Host proteases have been described to play essential roles in Ebola, HCV, HIV and influenza, such that specific protease inhibitors are able to reduce infection. RSV utilizes a host protease in its replication cycle but its potential as antiviral target is unknown...
August 17, 2017: Virology Journal
https://www.readbyqxmd.com/read/28814340/detection-and-genetic-characterization-of-canine-astroviruses-in-pet-dogs-in-guangxi-china
#3
Huabo Zhou, Lin Liu, Ruikai Li, Yifeng Qin, Qingli Fang, Vinod Rmt Balasubramaniam, Guojun Wang, Zuzhang Wei, Kang Ouyang, Weijian Huang, Ying Chen
BACKGROUND: Astroviruses (AstVs) have been reported to infect and cause gastroenteritis in most animal species. Human AstVs were regarded the causative agent of viral diarrhea in children. In dogs, little is known about the epidemiology and clinical significance of AstV infection. FINDINGS: In this study, we collected and tested 253 rectal swabs from pet dogs; of which 64 samples (25.3%) tested positive for AstVs with diarrhea and 15 more samples (5.9%) also was identified as AstVs, however without any clinical signs...
August 17, 2017: Virology Journal
https://www.readbyqxmd.com/read/28810884/a-novel-method-for-transmitting-southern-rice-black-streaked-dwarf-virus-to-rice-without-insect-vector
#4
Lu Yu, Jing Shi, Lianlian Cao, Guoping Zhang, Wenli Wang, Deyu Hu, Baoan Song
BACKGROUND: Southern rice black-streaked dwarf virus (SRBSDV) has spread from the south of China to the north of Vietnam in the past few years, and has severely influenced rice production. However, previous study of traditional SRBSDV transmission method by the natural virus vector, the white-backed planthopper (WBPH, Sogatella furcifera), in the laboratory, researchers are frequently confronted with lack of enough viral samples due to the limited life span of infected vectors and rice plants and low virus acquisition and inoculation efficiency by the vector...
August 15, 2017: Virology Journal
https://www.readbyqxmd.com/read/28807054/transcriptomic-profile-of-tobacco-in-response-to-tomato-zonate-spot-orthotospovirus-infection
#5
Changjun Huang, Yupeng Cun, Haiqin Yu, Zhijun Tong, Bingguang Xiao, Zhongbang Song, Bingwu Wang, Yongping Li, Yong Liu
BACKGROUND: Tomato zonate spot virus (TZSV), a dominant species of thrips-transmitted orthotospoviruses in Yunnan and Guangxi provinces in China, causes significant loss of yield in lots of crops and is a major threat to incomes of rural families. However, the detailed molecular mechanism of crop disease caused by TZSV remains obscure. METHODS: Next-generation sequencing (NGS)-based transcriptome analysis (RNA-seq) was performed to investigate and compare the gene expression changes in systemic leaves of tobacco upon infection with TZSV and mock-inoculated plants as a control...
August 14, 2017: Virology Journal
https://www.readbyqxmd.com/read/28807043/an-equine-herpesvirus-type-1-ehv-1-vector-expressing-rift-valley-fever-virus-rvfv-gn-and-gc-induces-neutralizing-antibodies-in-sheep
#6
Abdelrahman Said, Mona Elmanzalawy, Guanggang Ma, Armando Mario Damiani, Nikolaus Osterrieder
Rift Valley fever virus (RVFV) is an arthropod-borne bunyavirus that can cause serious and fatal disease in humans and animals. RVFV is a negative-sense RNA virus of the Phlebovirus genus in the Bunyaviridae family. The main envelope RVFV glycoproteins, Gn and Gc, are encoded on the M segment of RVFV and known inducers of protective immunity. In an attempt to develop a safe and efficacious RVF vaccine, we constructed and tested a vectored equine herpesvirus type 1 (EHV-1) vaccine that expresses RVFV Gn and Gc...
August 14, 2017: Virology Journal
https://www.readbyqxmd.com/read/28806976/quantitative-detection-of-human-malawi-polyomavirus-in-nasopharyngeal-aspirates-sera-and-feces-in-beijing-china-using-real-time-taqman-based-pcr
#7
Fen-Lian Ma, Dan-di Li, Tian-Li Wei, Jin-Song Li, Li-Shu Zheng
BACKGROUND: Human Malawi polyomavirus (MWPyV) was discovered in 2012, but its prevalence and clinical characteristics are largely unknown. METHODS: We used real-time TaqMan-based PCR to detect MWPyV in the feces (n = 174) of children with diarrhea, nasopharyngeal aspirates (n = 887) from children with respiratory infections, and sera (n = 200) from healthy adults, and analyzed its clinical characteristics statistically. All the MWPyV-positive specimens were also screened for other common respiratory viruses...
August 14, 2017: Virology Journal
https://www.readbyqxmd.com/read/28793904/the-impact-of-p53-on-the-early-stage-replication-of-retrovirus
#8
Michaela Kinnetz, Faris Alghamdi, Michael Racz, Wenwei Hu, Binshan Shi
BACKGROUND: The function of p53 in cancer biology has been studied extensively, but its role in anti-retrovirus infection has been elusive for many years. The restriction of retrovirus early stage replication by p53 was investigated in this study. METHOD: VSV-G pseudotyped retrovirus with GFP reporter gene was used to infect both HCT116 p53(+/+) cells and its isogenic p53 knockout HCT116 p53(-/-) cells. The infection was detected by flow cytometry. Reverse transcription products were quantified by real time PCR...
August 9, 2017: Virology Journal
https://www.readbyqxmd.com/read/28789701/a-novel-bladder-cancer-specific-oncolytic-adenovirus-by-cd46-and-its-effect-combined-with-cisplatin-against-cancer-cells-of-car-negative-expression
#9
Wenjuan Cao, Junqiang Tian, Chong Li, Yanjun Gao, Xingchen Liu, Jianzhong Lu, Yuhan Wang, Zhiping Wang, Robert S Svatek, Ronald Rodriguez
BACKGROUND: Conditionally replicative oncolytic adenoviruses (CRAds) display significant anti-tumor effects. However, the traditional adenovirus of serotype 5 (Ad5) entering cancer cells via coxsackie virus and adenovirus receptor (CAR) can't be utilized for bladder cancer with low expression of CAR, which limits the application of Ad5. METHODS: We utilized Ad5/F11p containing the chimeric fiber gene encoding the Ad5 fiber tail domain and Ad11p fiber shaft and knob domains to construct bladder cancer-specific chimeric type viruses Ad5/F11p-PSCAE-UPII-E1A, which can infect bladder cancer cells mediated by CD46 molecule...
August 8, 2017: Virology Journal
https://www.readbyqxmd.com/read/28789694/co-infection-of-two-reoviruses-increases-both-viruses-accumulation-in-rice-by-up-regulating-of-viroplasm-components-and-movement-proteins-bilaterally-and-rna-silencing-suppressor-unilaterally
#10
Shu Li, Tong Zhang, Yingzhi Zhu, Guohui Zhou
BACKGROUND: Synergism between southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV) not only aggravates disease symptoms but also enhances their vector acquisition efficiencies by increasing both viruses' titers in co-infected rice plants, which may exacerbate the epidemic of both viruses and cause significant damage to rice production. The molecular mechanism of viral synergism of these two viruses remains unexplored. METHODS: Single and double infection of SRBSDV and RRSV were obtained with the viruliferous white-backed planthopper and brown planthopper inoculation on four-leaf stage rice seedlings, respectively, under experimental condition...
August 8, 2017: Virology Journal
https://www.readbyqxmd.com/read/28784141/association-of-the-tandem-polymorphisms-rs148314165-rs200820567-in-tnfaip3-with-chronic-hepatitis-b-virus-infection-in-chinese-han-population
#11
Na Li, Ying Shi, Pingping Zhang, Jiao Sang, Fang Li, Huan Deng, Yi Lv, Qunying Han, Zhengwen Liu
BACKGROUND: Chronic hepatitis B virus (HBV) infection remains an important public health issue. A20, a ubiquitin-editing protein encoded by tumor necrosis factor alpha-inducible protein 3 (TNFAIP3) gene, is complicated in HBV infection and liver injury. The tandem polymorphisms (rs148314165, rs200820567), deletion T followed by a T to A transversion and collectively referred to as TT > A in TNFAIP3, may attenuate A20 expression. METHODS: The rs148314165 and rs200820567 polymorphisms were examined using PCR amplification followed by direct sequencing in 419 patients with chronic HBV infection, 77 HBV infection resolvers and 175 healthy controls of Chinese Han ethnicity...
August 7, 2017: Virology Journal
https://www.readbyqxmd.com/read/28774342/crimean-congo-hemorrhagic-fever-among-the-one-humped-camel-camelus-dromedaries-in-central-sudan
#12
Hajer M Suliman, Ibrahim A Adam, Shamseldin I Saeed, Sanaa A Abdelaziz, Eltahir M Haroun, Imadeldin E Aradaib
BACKGROUND: Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral zoonotic disease caused by Crimean-Congo hemorrhagic fever virus (CCHFV), a member of the genus Nairovirus in the family Bunyaviridae. CCHF is typically asymptomatic in animals but can be highly fatal in humans approaching case fatality rate of approximately 30%. In the present investigation, a cross sectional study was conducted to determine the prevalence of CCHF and to identify the potential risk factors associated with CCHFV seropositivity among the one-humped camel (Camelus dromedaries) in Central Sudan...
August 3, 2017: Virology Journal
https://www.readbyqxmd.com/read/28754134/occurrence-of-a-novel-mastrevirus-in-sugarcane-germplasm-collections-in-florida-guadeloupe-and-r%C3%A3-union
#13
Wardatou Boukari, Ricardo I Alcalá-Briseño, Simona Kraberger, Emmanuel Fernandez, Denis Filloux, Jean-Heinrich Daugrois, Jack C Comstock, Jean-Michel Lett, Darren P Martin, Arvind Varsani, Philippe Roumagnac, Jane E Polston, Philippe C Rott
BACKGROUND: In Africa and Asia, sugarcane is the host of at least seven different virus species in the genus Mastrevirus of the family Geminiviridae. However, with the exception of Sugarcane white streak virus in Barbados, no other sugarcane-infecting mastrevirus has been reported in the New World. Conservation and exchange of sugarcane germplasm using stalk cuttings facilitates the spread of sugarcane-infecting viruses. METHODS: A virion-associated nucleic acids (VANA)-based metagenomics approach was used to detect mastrevirus sequences in 717 sugarcane samples from Florida (USA), Guadeloupe (French West Indies), and Réunion (Mascarene Islands)...
July 28, 2017: Virology Journal
https://www.readbyqxmd.com/read/28754129/3-2-chloropropyl-amide-4-methoxy-n-phenylbenzamide-inhibits-expression-of-hpv-oncogenes-in-human-cervical-cancer-cell
#14
Fang Han, Yanping Li, Qiaoni Lu, Linlin Ma, Huiqiang Wang, Jiandong Jiang, Zhuorong Li, Yuhuan Li
BACKGROUND: Human papillomaviruses (HPVs) are the primary causative agents for cervical cancer, and HPV oncoproteins E6 and E7 are known to be the main reason for the onset and maintenance of the malignancies. Therefore, inhibition of viral E6 and E7 oncoproteins expression represents a viable strategy to cervical cancer therapies. This study is to evaluate the antiviral effect of a novel N-Phenylbenzamide derivative, 3-(2-Chloropropyl amide)-4-methoxy-N-phenylbenzamide (L17), against HPV16 in vitro and identify its associated mechanism of action in cervical cancer cells...
July 28, 2017: Virology Journal
https://www.readbyqxmd.com/read/28750646/metabolomics-of-rhabdomyosarcoma-cell-during-echovirus-30-infection
#15
Sarika Tiwari, Tapan N Dhole
BACKGROUND: Echovirus 30 (E30) causes acute aseptic meningitis. Viral replication requires energy and macromolecular precursors derived from the metabolic network of the host cell. The effect of viral infection within a host cell metabolic activity remains unclear. METHODS: To gain an insight into cell-virus interaction during E30 infection we used a human rhabdomyosarcoma cell line. In a new approach to metabolomics, (1H) NMR was used to measure the level of various cellular metabolites at different times of infection and morphological examination of the cells...
July 27, 2017: Virology Journal
https://www.readbyqxmd.com/read/28750638/erratum-to-prevalence-of-anelloviruses-ttv-ttmdv-and-ttmv-in-healthy-blood-donors-and-in-patients-infected-with-hbv-or-hcv-in-qatar
#16
Ahmed A Al-Qahtani, Enas S Alabsi, Raed AbuOdeh, Lukman Thalib, Mohamed E El Zowalaty, Gheyath K Nasrallah
No abstract text is available yet for this article.
July 27, 2017: Virology Journal
https://www.readbyqxmd.com/read/28747230/full-length-genome-sequence-analysis-of-enzootic-nasal-tumor-virus-isolated-from-goats-in-china
#17
Yapeng He, Qi Zhang, Jing Wang, Man Zhou, Mingzhe Fu, Xingang Xu
BACKGROUND: Enzootic nasal tumor virus (ENTV) is a betaretrovirus of sheep (ENTV-1) and goats (ENTV-2) associated with neoplastic transformation of epithelial cells of the ethmoid turbinate. Confirmation of the role of ENTV in the pathogenesis of enzootic nasal adenocarcinoma (ENA) has yet to be resolved due to the inability to culture the virus. Very little is known about the prevalence of this disease, particularly in China. METHODS: To evaluate the genetic diversity of ENTV-2 from Shaanxi province of China, the complete genome sequence of four isolates from Shaanxi province was determined by RT-PCR...
July 26, 2017: Virology Journal
https://www.readbyqxmd.com/read/28747200/rubella-herpes-simplex-virus-type-2-and-preeclampsia
#18
Shimos A Alshareef, Ahmed M Eltom, Abubakr M Nasr, Hamdan Z Hamdan, Ishag Adam
BACKGROUND: Preeclampsia is a major health problem. Although, the pathophysiology of preeclampsia is not fully understood, there are recent studies on association between infections and preeclampsia. OBJECTIVE: The aim of the present study was to investigate the association between maternal seropositivity of rubella, Herpes simplex virus type 2 (HSV-2) and preeclampsia. METHOD: A case -controls study (90 women in each arm) was conducted at Saad Abualila Maternity Hospital, Khartoum, Sudan...
July 26, 2017: Virology Journal
https://www.readbyqxmd.com/read/28743260/molecular-evolution-of-two-asymptomatic-echovirus-6-strains-that-constitute-a-novel-branch-of-recently-epidemic-echovirus-6-in-china
#19
Hao Sun, Xiaoqin Huang, Keqin Lin, Kai Huang, Jiayou Chu, Zhaoqing Yang, Shaohui Ma
BACKGROUND: Echovirus 6 (E6) infections are associated with aseptic meningitis and acute flaccid paralysis (AFP). But some infections, sometimes most of them, are asymptomatic. The mechanism of E6 virulence is unknown. Analyses of the molecular evolution of asymptomatic E6 may help understand why the infections show different manifestations. METHODS: Ninety-six stool samples of healthy children in Yunnan, China were collected and two E6 strains were isolated from them...
July 25, 2017: Virology Journal
https://www.readbyqxmd.com/read/28738896/real-time-reverse-transcription-pcr-based-sequencing-independent-pathotyping-of-eurasian-avian-influenza-a-viruses-of-subtype-h7
#20
Annika Graaf, Martin Beer, Timm Harder
Low pathogenic avian influenza viruses (LPAIV) of the subtypes H5 and H7 are known to give rise to highly pathogenic (HP) phenotypes by spontaneous insertional mutations which convert a monobasic trypsin-sensitive endoproteolytical cleavage site (CS) within the hemagglutinin (HA) protein into a polybasic subtilisin-sensitive one. Sporadic outbreaks of notifiable LPAIV H7 infections are continuously recorded in Europe and in Asia, and some lineages showed zoonotic transmission. De novo generation of HPAIV H7 from LPAIV precursors has been reported several times over the past decade...
July 24, 2017: Virology Journal
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