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Virology Journal

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https://www.readbyqxmd.com/read/28438210/specific-and-quantitative-detection-of-human-polyomaviruses-bkpyv-and-jcpyv-in-the-healthy-pakistani-population
#1
Iqra Hussain, Fareeda Tasneem, Muhammed Umer, Ayesha Pervaiz, Muslim Raza, Muhammad Imran Arshad, Naveed Shahzad
BACKGROUND: The BK Polyomavirus (BKPyV) and JC polyomavirus (JCPyV) infections are widespread in human population and have been associated with severe kidney and brain disorders, respectively. The viruses remain latent primarily in reno-urinary tract, reactivating only in case of a compromised immune system. The seroepidemiology and molecular prevalence of BKPyV and JCPyV have been widely studied both in healthy and immunocompromised patients worldwide. However, data regarding the prevalence of these viruses in the immunocompetent or apparently healthy Pakistani population is lacking...
April 24, 2017: Virology Journal
https://www.readbyqxmd.com/read/28431572/hepatitis-c-virus-ns4b-protein-induces-epithelial-mesenchymal-transition-by-upregulation-of-snail
#2
Bicheng Hu, Shenggao Xie, Yuqian Hu, Wen Chen, Xiaofan Chen, Yi Zheng, Xinxing Wu
BACKGROUND: Chronic hepatitis C virus (HCV) infection is an important cause of hepatocellular carcinoma (HCC). Epithelial to mesenchymal transition (EMT) is a key process associated with tumor metastasis and poor prognosis. HCV infection, HCV core and NS5A protein could induce EMT process, but the role of NS4B on EMT remains poorly understood. METHODS: We overexpressed HCV NS4B protein in HepG2 cells or Huh7.5.1 cells infected by HCVcc, the E-cadherin expression, N-cadherin expression and the EMT-associated transcriptional factor Snail were determined...
April 21, 2017: Virology Journal
https://www.readbyqxmd.com/read/28431571/unique-lcr-variations-among-lineages-of-hpv16-18-and-45-isolates-from-women-with-normal-cervical-cytology-in-ghana
#3
Adolf K Awua, Richard M K Adanu, Edwin K Wiredu, Edwin A Afari, Vanessa A Zubuch, Richard H Asmah, Alberto Severini
BACKGROUND: In addition to being useful for classification, sequence variations of human Papillomavirus (HPV) genotypes have been implicated in differential oncogenic potential and a differential association with the different histological forms of invasive cervical cancer. These associations have also been indicated for HPV genotype lineages and sub-lineages. In order to better understand the potential implications of lineage variation in the occurrence of cervical cancers in Ghana, we studied the lineages of the three most prevalent HPV genotypes among women with normal cytology as baseline to further studies...
April 21, 2017: Virology Journal
https://www.readbyqxmd.com/read/28431558/epidemiology-and-genotype-distribution-of-human-papillomavirus-hpv-in-southwest-china-a-cross-sectional-five-years-study-in-non-vaccinated-women
#4
Yishu Tang, Lan Zheng, Shuangshuang Yang, Bing Li, Huiting Su, Li-Ping Zhang
BACKGROUND: Large-size data on type-specific HPV prevalence in Southwest China are required to estimate the cervical cancer burden in the country and to prepare for HPV-based cervical screening program and further HPV vaccination of China. This HPV study is a pooled analysis of data from five years in Chongqing of China, which is cross-sectional in design using data collecting. RESULTS: The positivity of HPV was 26.2% (10542/40311), single type was 25.7% (10360/40311), multiple type was 8...
April 21, 2017: Virology Journal
https://www.readbyqxmd.com/read/28420392/tissue-tropisms-infection-kinetics-histologic-lesions-and-antibody-response-of-the-mr766-strain-of-zika-virus-in-a-murine-model
#5
Anna B Kawiecki, E Handly Mayton, M Fausta Dutuze, Brad A Goupil, Ingeborg M Langohr, Fabio Del Piero, Rebecca C Christofferson
BACKGROUND: The appearance of severe Zika virus (ZIKV) disease in the most recent outbreak has prompted researchers to respond through the development of tools to quickly characterize transmission and pathology. We describe here another such tool, a mouse model of ZIKV infection and pathogenesis using the MR766 strain of virus that adds to the growing body of knowledge regarding ZIKV kinetics in small animal models. METHODS: We infected mice with the MR766 strain of ZIKV to determine infection kinetics via serum viremia...
April 18, 2017: Virology Journal
https://www.readbyqxmd.com/read/28420390/a-novel-viewrna-in-situ-hybridization-method-for-the-detection-of-the-dynamic-distribution-of-classical-swine-fever-virus-rna-in-pk15-cells
#6
COMPARATIVE STUDY
Qianyi Zhang, Lu Xu, Yujie Zhang, Tuanjie Wang, Xingqi Zou, Yuanyuan Zhu, Yan Zhao, Cui Li, Kai Chen, Yongfang Sun, Junxiang Sun, Qizu Zhao, Qin Wang
BACKGROUND: Classical swine fever (CSF) is a highly contagious fatal infectious disease caused by classical swine fever virus (CSFV). A better understanding of CSFV replication is important for the study of pathogenic mechanism of CSF. With the development of novel RNA in situ Hybridization method, quantitatively localization and visualization of the virus RNA molecular in cultured cell or tissue section becomes very important tool to address these pivotal pathogenic questions. In this study, we established ViewRNA ISH method to reveal the dynamic distribution of CSFV RNA in PK15 cells...
April 18, 2017: Virology Journal
https://www.readbyqxmd.com/read/28410597/the-role-of-nuclear-localization-signal-in-parvovirus-life-cycle
#7
REVIEW
Peng Liu, Shun Chen, Mingshu Wang, Anchun Cheng
Parvoviruses are small, non-enveloped viruses with an approximately 5.0 kb, single-stranded DNA genome. Usually, the parvovirus capsid gene contains one or more nuclear localization signals (NLSs), which are required for guiding the virus particle into the nucleus through the nuclear pore. However, several classical NLSs (cNLSs) and non-classical NLSs (ncNLSs) have been identified in non-structural genes, and the ncNLSs can also target non-structural proteins into the nucleus. In this review, we have summarized recent research findings on parvovirus NLSs...
April 14, 2017: Virology Journal
https://www.readbyqxmd.com/read/28410592/study-of-sv40-large-t-antigen-nucleotide-specificity-for-dna-unwinding
#8
Damian Wang, Ana Lucia Álvarez-Cabrera, Xiaojiang S Chen
BACKGROUND: Simian Virus 40 (SV40) Large Tumor Antigen (LT) is an essential enzyme that plays a vital role in viral DNA replication in mammalian cells. As a replicative helicase and initiator, LT assembles as a double-hexamer at the SV40 origin to initiate genomic replication. In this process, LT converts the chemical energy from ATP binding and hydrolysis into the mechanical work required for unwinding replication forks. It has been demonstrated that even though LT primarily utilizes ATP to unwind DNA, other NTPs can also support low DNA helicase activity...
April 14, 2017: Virology Journal
https://www.readbyqxmd.com/read/28407817/preliminary-study-of-the-ul55-gene-based-on-infectious-chinese-virulent-duck-enteritis-virus-bacterial-artificial-chromosome-clone
#9
Ying Wu, Yangguang Li, Mingshu Wang, Kunfeng Sun, Renyong Jia, Shun Chen, Dekang Zhu, Mafeng Liu, Qiao Yang, Xinxin Zhao, Xiaoyue Chen, Anchun Cheng
BACKGROUND: Lethal Duck Enteritis Virus (DEV) infection can cause high morbidity and mortality of many species of waterfowl within the order Anseriformes. However, little is known about the function of viral genes including the conserved UL55 gene among alpha herpes virus due to the obstacles in maintenance and manipulation of DEV genome in host cells. METHODS: In this paper, we constructed an infectious bacteria artificial chromosome (BAC) clone of the lethal clinical isolate duck enteritis virus Chinese virulent strain (DEV CHv) by inserting a transfer vector containing BAC mini-F sequence and selection marker EGFP into UL23 gene using homologous recombination...
April 13, 2017: Virology Journal
https://www.readbyqxmd.com/read/28407787/glucose-regulated-protein-78-demonstrates-antiviral-effects-but-is-more-suitable-for-hepatocellular-carcinoma-prevention-in-hepatitis-b
#10
Nai Q Zheng, Zi H Zheng, Hai X Xu, Ming X Huang, Xiao M Peng
BACKGROUND: Hepatitis B virus (HBV) is the leading cause of liver cirrhosis and hepatocellular carcinoma in Asia and Africa. Existing antivirals cannot cure HBV or eliminate risk of hepatocellular carcinoma. Glucose-regulated protein 78 (GRP78) can inhibit HBV replication, but promote virion secretion and hepatocellular cancer cell invasion. For these reasons, the overall effect of GRP78 on HBV production and whether to utilize the HBV replication-inhibitory effect of GRP78 up-regulation or the hepatocellular cancer cell invasion-inhibitory effect of its down-regulation were further investigated in order to improve the efficacy of current antiviral therapy...
April 13, 2017: Virology Journal
https://www.readbyqxmd.com/read/28403874/intranasal-immunization-of-pigs-with-porcine-reproductive-and-respiratory-syndrome-virus-like-particles-plus-2-3-cgamp-vaccigrade%C3%A2-adjuvant-exacerbates-viremia-after-virus-challenge
#11
Alexandria Van Noort, April Nelsen, Angela E Pillatzki, Diego G Diel, Feng Li, Eric Nelson, Xiuqing Wang
BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) causes reproductive failure in pregnant sows and acute respiratory disease in young pigs. It is a leading infectious agent of swine respiratory complex, which has significant negative economic impact on the swine industry. Commercial markets currently offer both live attenuated and killed vaccines; however, increasing controversy exists about their efficacy providing complete protection. Virus-like particles (VLPs) possess many desirable features of a potent vaccine candidate and have been proven to be highly immunogenic and protective against virus infections...
April 12, 2017: Virology Journal
https://www.readbyqxmd.com/read/28399888/a-guinea-pig-model-of-zika-virus-infection
#12
Mukesh Kumar, Keeton K Krause, Francine Azouz, Eileen Nakano, Vivek R Nerurkar
BACKGROUND: Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemic of Zika virus (ZIKV). Here we report that immunocompetent guinea pigs are susceptible to infection by a contemporary American strain of ZIKV. METHODS: Dunkin-Hartley guinea pigs were inoculated with 10(6) plaque-forming units of ZIKV via subcutaneous route and clinical signs were observed. Viremia, viral load in the tissues, anti-ZIKV neutralizing antibody titer, and protein levels of multiple cytokine and chemokines were analyzed using qRT-PCR, plaque assay, plaque reduction neutralization test (PRNT) and multiplex immunoassay...
April 11, 2017: Virology Journal
https://www.readbyqxmd.com/read/28390433/evaluation-of-the-performance-of-human-papillomavirus-testing-in-paired-urine-and-clinician-collected-cervical-samples-among-women-aged-over-30%C3%A2-years-in-bhutan
#13
Ugyen Tshomo, Silvia Franceschi, Tshokey Tshokey, Tashi Tobgay, Iacopo Baussano, Vanessa Tenet, Peter J F Snijders, Tarik Gheit, Massimo Tommasino, Alex Vorsters, Gary M Clifford
BACKGROUND: Urine sampling may offer a less invasive solution than cervical sampling to test for human papillomavirus (HPV) for HPV vaccine impact monitoring. METHODS: Paired samples of urine and exfoliated cervical cells were obtained for 89 women with history of high-risk (HR) HPV-positive normal cytology in Bhutan. Urine sampling protocol included self-collection of first-void urine immediately into a conservation medium and procedures to optimize DNA yield. Colposcopical abnormalities were biopsied...
April 8, 2017: Virology Journal
https://www.readbyqxmd.com/read/28388934/isolation-and-identification-of-a-new-strain-of-hirame-rhabdovirus-hirrv-from-japanese-flounder-paralichthys-olivaceus-in-china
#14
Jialin Zhang, Xiaoqian Tang, Xiuzhen Sheng, Jing Xing, Wenbin Zhan
BACKGROUND: Hirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe. METHODS: In this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong province, China. Cell isolation, electron microscopic observation, RT-PCR detection and phylogenetic analysis were used for virus identification. Further, artificial infection experiment was conducted for virulence testing...
April 7, 2017: Virology Journal
https://www.readbyqxmd.com/read/28388926/proof-of-concept-study-profile-of-circulating-micrornas-in-bovine-serum-harvested-during-acute-and-persistent-fmdv-infection
#15
Carolina Stenfeldt, Jonathan Arzt, George Smoliga, Michael LaRocco, Joseph Gutkoska, Paul Lawrence
BACKGROUND: Changes in the levels of circulating microRNAs (miRNAs) in the serum of humans and animals have been detected as a result of infection with a variety of viruses. However, to date, such a miRNA profiling study has not been conducted for foot-and-mouth disease virus (FMDV) infection. METHODS: The relative abundance of 169 miRNAs was measured in bovine serum collected at three different phases of FMDV infection in a proof-of-concept study using miRNA PCR array plates...
April 7, 2017: Virology Journal
https://www.readbyqxmd.com/read/28388925/a-robust-and-cost-effective-approach-to-sequence-and-analyze-complete-genomes-of-small-rna-viruses
#16
Kiril M Dimitrov, Poonam Sharma, Jeremy D Volkening, Iryna V Goraichuk, Abdul Wajid, Shafqat Fatima Rehmani, Asma Basharat, Ismaila Shittu, Tony M Joannis, Patti J Miller, Claudio L Afonso
BACKGROUND: Next-generation sequencing (NGS) allows ultra-deep sequencing of nucleic acids. The use of sequence-independent amplification of viral nucleic acids without utilization of target-specific primers provides advantages over traditional sequencing methods and allows detection of unsuspected variants and co-infecting agents. However, NGS is not widely used for small RNA viruses because of incorrectly perceived cost estimates and inefficient utilization of freely available bioinformatics tools...
April 7, 2017: Virology Journal
https://www.readbyqxmd.com/read/28388922/viral-and-serological-kinetics-in-zika-virus-infected-patients-in-south-korea
#17
Young Eui Jeong, Go-Woon Cha, Jung Eun Cho, Eun Ju Lee, Youngmee Jee, Won-Ja Lee
Zika virus is a mosquito-borne flavivirus that causes clinical symptoms similar to those observed in dengue and chikungunya virus infections. The Korea Centers for Disease Control and Prevention initiated laboratory testing using a real-time reverse transcription-polymerase chain reaction in January 2016. More than 1,000 suspected cases of infection were tested and nine were confirmed as imported cases of Zika virus infection from January to July 2016. The travel destinations of the infected individuals were Brazil, Philippines, Viet Nam, Guatemala, Puerto Rico, and the Dominican Republic...
April 7, 2017: Virology Journal
https://www.readbyqxmd.com/read/28381285/new-oligonucleotide-microarray-for-rapid-diagnosis-of-avian-viral-diseases
#18
Kulyaisan T Sultankulova, Nurlan S Kozhabergenov, Vitaliy M Strochkov, Yerbol D Burashev, Kamshat A Shorayeva, Olga V Chervyakova, Nurkuisa M Rametov, Nurlan T Sandybayev, Abylay R Sansyzbay, Mukhit B Orynbayev
BACKGROUND: We developed a new oligonucleotide microarray comprising 16 identical subarrays for simultaneous rapid detection of avian viruses: avian influenza virus (AIV), Newcastle disease virus (NDV), infection bronchitis virus (IBV), and infectious bursal disease virus (IBDV) in single- and mixed-virus infections. The objective of the study was to develop an oligonucleotide microarray for rapid diagnosis of avian diseases that would be used in the course of mass analysis for routine epidemiological surveillance owing to its ability to test one specimen for several infections...
April 5, 2017: Virology Journal
https://www.readbyqxmd.com/read/28376852/antiviral-efficacy-of-entecavir-for-hepatitis-b-virus-rta181v-t-mutants
#19
Ping Li, Jiabao Geng, Wei Li, Xiaobing Xu, Xin Zhang, Wenkai Zheng, Yuecheng Yu, Zhiguo Yang, Maorong Wang
BACKGROUND: The amino acid substitution at position 181 of the Hepatitis B virus (HBV) polymerase is a multi-drug resistance affecting both the L-nucleoside and acyclic phosphonate nucleotide groups. Data is limited on the efficacy of entecavir (ETV) rescuing chronic hepatitis B (CHB) patients with rtA181T/V mutation. METHODS: Thirty-one patients with rtA181T/V mutation and 25 patients with rtA181T/V and rtN236T mutation were enrolled. Virological, serological and biochemical outcomes of ETV rescue therapy over 12 months in CHB patients with rtA181T/V mutation strains were investigated...
April 4, 2017: Virology Journal
https://www.readbyqxmd.com/read/28376821/emergence-of-mosaic-recombinant-strains-potentially-associated-with-vaccine-jxa1-r-and-predominant-circulating-strains-of-porcine-reproductive-and-respiratory-syndrome-virus-in-different-provinces-of-china
#20
Huajian Zhao, Qinggong Han, Lei Zhang, Zhiyong Zhang, Yufeng Wu, Hong Shen, Ping Jiang
BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) has caused several outbreaks in China since 2006. However, the genetic diversity of PRRSV in China has greatly increased by rapid evolution or recombination events. Modified live-attenuated vaccines are widely used to control this disease worldwide. Although the risk and inefficacy of the vaccine has been reported, the genetic diversity between epidemic field strains and vaccine strains in China has not been completely elucidated...
April 4, 2017: Virology Journal
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