journal
MENU ▼
Read by QxMD icon Read
search

FEBS Journal

journal
https://www.readbyqxmd.com/read/28650586/a-complete-thermodynamic-analysis-of-enzyme-turnover-links-the-free-energy-landscape-to-enzyme-catalysis
#1
Hannah Bl Jones, Stephen A Wells, Erica J Prentice, Anthony Kwok, Liyin L Liang, Vickery L Arcus, Christopher R Pudney
Our understanding of how enzymes work is colored by static structure depictions where the enzyme scaffold is presented as either immobile, or in equilibrium between well-defined static conformations. Proteins however exhibit a large degree of motion over a broad range of timescales and magnitudes and this is defined thermodynamically by the enzyme free energy landscape (FEL). The role and importance of enzyme motion is extremely contentious. Much of the challenge is in the experimental detection of so called 'conformational sampling' involved in enzyme turnover...
June 26, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28646582/comprehensive-mathematical-model-of-oxidative-phosphorylation-valid-for-physiological-up-to-pathological-conditions
#2
Margit Heiske, Thierry Letellier, Edda Klipp
We developed a mathematical model of oxidative phosphorylation (OXPHOS) that allows for a precise description of mitochondrial function with respect to the respiratory flux and the ATP production. The model reproduced flux-force relationships under various experimental conditions (state 3 and 4, uncoupling, shortage of respiratory substrate) as well as time courses, exhibiting correct P/O ratios. The model was able to reproduce experimental threshold curves for perturbations of the respiratory chain complexes, the F1 F0 -ATP synthase, the ADP/ATP carrier, the phosphate/OH carrier, and the proton leak...
June 24, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28644560/identification-and-characterization-of-ars-like-sequences-as-putative-origin-s-of-replication-in-human-malaria-parasite-plasmodium-falciparum
#3
Meetu Agarwal, Krishanu Bhowmick, Kushal Shah, Annangarachari Krishnamachari, Suman Kumar Dhar
DNA replication is a fundamental process in genome maintenance, and initiates from several genomic sites (origins) in eukaryotes. In S. cerevisiae, conserved sequences known as Autonomously Replicating Sequences (ARSs) provide a landing pad for the Origin Recognition Complex (ORC), leading to replication initiation. Although origins from higher eukaryotes share some common sequence features, the definitive genomic organization of these sites remains elusive. The human malaria parasite Plasmodium falciparum undergoes multiple rounds of DNA replication; therefore, control of initiation events is crucial to ensure proper replication...
June 23, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28636167/berberine-ameliorates-collagen-induced-arthritis-in-rats-by-suppressing-th17-cell-responses-via-inducing-cortistatin-in-the-gut
#4
Mengfan Yue, Yufeng Xia, Can Shi, Chunge Guan, Yunfan Li, Rui Liu, Zhifeng Wei, Yue Dai
Berberine, an isoquinoline alkaloid, has been reported to ameliorate various autoimmune diseases including rheumatoid arthritis by oral administration. However, its mechanism remains mysterious due to an extremely low bioavailability. The fact that berberine is easy to accumulate in gut, the largest endocrine organ in the body, attracts us to explore its anti-arthritic mechanism in view of the induction of intestinal immunosuppressive neuropeptides. In this study, berberine (200 mg/kg, i.g.) was shown to ameliorate collagen-induced arthritis in rats, which was manifested by the reduction of clinical signs and joint destruction, as well as marked down-regulation of Th17 cell frequency and IL-17 level in blood...
June 21, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28636114/set-i2pp2a-overexpression-induces-phenotypic-molecular-and-metabolic-alterations-in-an-oral-keratinocyte-cell-line
#5
Lays M Sobral, Ricardo D Coletta, Luciane C Alberici, Carlos Curti, Andréia Machado Leopoldino
The multifunctional SET/I2PP2A protein is known to be overexpressed in head and neck squamous cell carcinoma. However, SET has been reported to have apparently conflicting roles in promoting cancer cell survival under oxidative stress conditions and preventing invasion and metastasis, complicating efforts to understand the contribution of SET to carcinogenesis. In the present study, we overexpressed SET in a spontaneously immortalized oral keratinocyte cell line (NOK-SI SET) and demonstrated that SET up-regulation alone was sufficient to transform cells...
June 21, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28631426/hsp90%C3%AE-regulates-atm-and-nbn-functions-in-sensing-and-repair-of-dna-double-strand-breaks
#6
Rosa Pennisi, Antonio Antoccia, Stefano Leone, Paolo Ascenzi, Alessandra di Masi
The molecular chaperone heat shock protein 90 (Hsp90α) regulates cells proteostasis and mitigates the harmful effects of endogenous and exogenous stressors on the proteome. Indeed, the inhibition of Hsp90α ATPase activity affects the cellular response to ionizing radiation (IR). Although the interplay between Hsp90α and several DNA damage response (DDR) proteins has been reported, its role in the DDR is still unclear. Here, we show that ATM and NBN, but not 53BP1, RAD50, and MRE11, are Hsp90α clients as the Hsp90α inhibitor 17-(allylamino)-17-demethoxygeldanamycin (17-AAG) induces ATM and NBN polyubiquitination and proteosomal degradation in normal fibroblasts and lymphoblastoid cells lines...
June 20, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28631368/crispr-cas9-mediated-loss-of-fgf5-function-increases-wool-staple-length-in-sheep
#7
Wen-Rong Li, Chen-Xi Liu, Xue-Mei Zhang, Lei Chen, Xin-Rong Peng, San-Gang He, Jia-Peng Lin, Bin Han, Li-Qin Wang, Jun-Cheng Huang, Ming-Jun Liu
Fibroblast growth factor 5 (FGF5) regulates hair length in humans and a variety of other animals. To investigate whether FGF5 has similar effects in sheep, we used CRISPR/Cas9 to generate loss-of-function mutations with the FGF5 gene in Chinese Merino sheep. A total of 16 lambs were identified with genetic mutations within the targeting locus: 13 lambs had biallelic modifications and 3 lambs had monoallelic modifications. Characterization of the modifications revealed that 13 were frame shift mutations that led to premature termination; the other 3 were in-frame deletions...
June 20, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28627136/enhancer-of-rudimentary-homologue-interacts-with-scaffold-attachment-factor-b-at-the-nuclear-matrix-to-regulate-sr-protein-phosphorylation
#8
Sotiria Drakouli, Aggeliki Lyberopoulou, Maria Papathanassiou, Ilias Mylonis, Eleni Georgatsou
Scaffold Attachment Factor B1 (SAFB1) is an integral component of the nuclear matrix of vertebrate cells.It binds to DNA on scaffold/matrix attachment region (S/MAR) elements, to RNA, and to a multitude of different proteins, affecting, as has been extensively substantiated, basic cellular activities such as transcription, splicing and DNA damage repair (DDR). In this study, we show that Enhancer of Rudimentary Homologue (ERH) is a new molecular partner of SAFB1 and its 70% homologous paralogue, Scaffold Attachment Factor B2 (SAFB2)...
June 19, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28627025/novel-rankl-de-loop-mutants-antagonize-rank-mediated-osteoclastogenesis
#9
Yizhou Wang, Aart H G van Assen, Carlos R Reis, Rita Setroikromo, Ronald van Merkerk, Ykelien L Boersma, Robbert H Cool, Wim J Quax
Bone is a dynamic tissue that is maintained by continuous renewal. An imbalance in bone resorption and bone formation can lead to a range of disorders, such as osteoporosis. The Receptor Activator of NF-κB (RANK)-RANK ligand (RANKL) pathway plays a major role in bone remodeling. Here, we investigated the effect of mutations at position I248 in the DE-loop of murine RANKL on the interaction of RANKL with RANK, and subsequent activation of osteoclastogenesis. Two single mutants, RANKL I248Y and I248K, were found to maintain binding and have the ability to reduce wild type RANKL-induced osteoclastogenesis...
June 19, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28627020/structural-studies-of-a-hyperthermophilic-thymidylate-kinase-enzyme-reveal-conformational-sub-states-along-the-reaction-coordinate
#10
Ansuman Biswas, Arpit Shukla, Santosh Kumar Chaudhary, R Santhosh, Jeyaraman Jeyakanthan, Kanagaraj Sekar
Thymidylate kinase (TMK) is a key enzyme which plays an important role in DNA synthesis. It belongs to the family of nucleoside monophosphate kinases (NMPKs), several of which undergo structure-encoded conformational changes to perform their function. However, the absence of three dimensional structures for all the different reaction intermediates of a single TMK homolog hinders a clear understanding of its functional mechanism. We herein report the different conformational states along the reaction coordinate of a hyperthermophilic TMK from Aquifex aeolicus, determined via x-ray diffraction and further validated through normal mode studies...
June 19, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28627018/the-nucleotide-free-state-of-heterotrimeric-g-proteins-%C3%AE-subunit-adopts-a-highly-stable-conformation
#11
Sai Krishna Andhirka, Ravichandran Vignesh, Gopala Krishna Aradhyam
Deciphering the mechanism of activation of heterotrimeric G proteins by their cognate receptors continues to be an intriguing area of research. The recently solved crystal structure of the ternary complex captured the receptor-bound α-subunit in an open conformation, without bound nucleotide has improved our understanding of the activation process. Despite these advancements, the mechanism by which the receptor causes GDP release from the α-subunit remains elusive. To elucidate the mechanism of activation, we studied guanine nucleotide-induced structural stability of the α-subunit (in response to thermal/chaotrope-mediated stress)...
June 19, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28626941/binding-of-canonical-wnt-ligands-to-their-receptor-complexes-occurs-in-ordered-plasma-membrane-environments
#12
Erdinc Sezgin, Yagmur Azbazdar, Xue Wen Ng, Cathleen Teh, Kai Simons, Gilbert Weidinger, Thorsten Wohland, Christian Eggeling, Gunes Ozhan
While the cytosolic events of Wnt/β-catenin signaling (canonical Wnt signaling) pathway have been widely studied, only little is known about the molecular mechanisms involved in Wnt binding to its receptors at the plasma membrane. Here, we reveal the influence of the immediate plasma membrane environment on the canonical Wnt-receptor interaction. While the receptors are distributed both in ordered and disordered environments, Wnt binding to its receptors selectively occurs in more ordered membrane environments which appear to co-internalize with the Wnt-receptor complex...
June 19, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28622459/structural-plasticity-among-glutathione-transferase-phi-members-natural-combination-of-catalytic-residues-confers-dual-biochemical-activities
#13
Henri Pégeot, Sandrine Mathiot, Thomas Perrot, Frédéric Gense, Arnaud Hecker, Claude Didierjean, Nicolas Rouhier
The glutathione transferase (GST) gene family is divided into fourteen classes in photosynthetic organisms. Among them, the Phi class (GSTF) is composed of a large number of genes that are often induced in response to environmental constraints due to their ability to detoxify xenobiotics, to their peroxidase activity and to their involvement in the biosynthesis and/or transport of secondary metabolites. However, the exact functions of GSTFs from many plants including Populus trichocarpa are unknown. Here, following GSTF1 characterization, we have performed a comparative analysis of the seven other GSTFs found in poplar by systematically evaluating the biochemical and enzymatic properties of the corresponding recombinant proteins and of variants mutated for active site residues and by determining the three-dimensional structures of several representatives...
June 16, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28622436/mechanistic-basis-for-increased-human-gene-targeting-by-promoterless-vectors-roles-of-homology-arms-and-rad54-paralogs
#14
Shinta Saito, Aya Kurosawa, Noritaka Adachi
Gene targeting by homologous recombination provides the definitive tool for analyzing gene function. Promoterless vectors, which do not possess a promoter to drive marker gene expression, confer higher targeting efficiencies than conventional vectors due to reduced number of drug-resistant clones. We here show that gene-targeting efficiency is typically ≥ 25% with the use of exon-trapping-type promoterless vectors in a human diploid cell line, Nalm-6. The efficiency of exon-trapping gene targeting was correlated with the level of target gene expression when a 2A peptide sequence was linked to the marker gene...
June 16, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28618199/lipid-interactions-modulate-the-structural-and-antigenic-properties-of-the-c-terminal-domain-of-the-malaria-antigen-merozoite-surface-protein-2
#15
Sreedam C Das, Rodrigo A V Morales, Jeffrey Seow, Krishnarjuna Bankala, Ravindu Dissanayake, Robin F Anders, Christopher A MacRaild, Raymond S Norton
Merozoite surface protein 2 (MSP2) is a highly abundant, GPI-anchored antigen on the malaria parasite Plasmodium falciparum. MSP2 induces an immune response in the context of natural infections and vaccine trials, and these responses are associated with protection from parasite infection. Recombinant MSP2 is highly disordered in solution but antigenic analyses suggest that it is more ordered on the merozoite surface. We have shown previously that the interaction of recombinant full-length MSP2 with lipid surfaces induces a conformational change in the conserved N-terminal region of MSP2, which contributes to epitope masking in this region...
June 15, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28618168/streptococcus-pyogenes-quinolinate-salvage-pathway-structural-and-functional-studies-of-quinolinate-phosphoribosyl-transferase-and-nh3-dependent-nad-synthetase
#16
William T Booth, Trevor L Morris, David P Mysona, Milan J Shah, Linda K Taylor, Taylor W Karlin, Kathryn Clary, Karolina A Majorek, Lesa R Offermann, Maksymilian Chruszcz
Streptococcus pyogenes, also known as Group A Strep (GAS), is an obligate human pathogen that is responsible for millions of infections and numerous deaths per year. Infection manifestations can range from simple, acute pharyngitis to more complex, necrotizing fasciitis. To date, most treatments for GAS infections involve the use of common antibiotics including tetracycline and clindamycin. Unfortunately, new strains have been identified that are resistant to these drugs therefore, new targets must be identified to treat drug resistant strains...
June 15, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28618157/liver-microsomal-lipid-enhances-the-activity-and-redox-coupling-of-co-localised-cytochrome-p450-reductase-cytochrome-p450-3a4-in-nanodiscs
#17
Kang-Cheng Liu, John M X Hughes, Sam Hay, Nigel S Scrutton
The haem-containing monooxygenase cytochrome P450 3A4 (CYP3A4) and its redox partner NADPH-dependent cytochrome P450 oxidoreductase (CPR) are among the most important enzymes in human liver for metabolising drugs and xenobiotic compounds. They are membrane-bound in the endoplasmic reticulum (ER). How ER co-localisation and the complex ER phospholipid composition influence enzyme activity are not well understood. CPR and CYP3A4 were incorporated into phospholipid bilayer nanodiscs, both singly, and together in a 1:1 ratio, to investigate the significance of membrane insertion and the influence of varying membrane composition on steady-state reaction kinetics...
June 15, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28613457/live-cell-imaging-of-cell-signaling-using-genetically-encoded-fluorescent-reporters
#18
REVIEW
Qiang Ni, Sohum Mehta, Jin Zhang
Synergistic advances in fluorescent protein (FP) engineering and live-cell imaging techniques in recent years have fueled the concurrent development and application of genetically encoded fluorescent reporters that are tailored for tracking signaling dynamics in living systems over multiple length and time scales. These biosensors are uniquely suited for this challenging task, owing to their specificity, sensitivity, and versatility, as well as to the non-invasive and non-destructive nature of fluorescence and the power of genetic encoding...
June 14, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28605567/mir-30a-increases-mdsc-differentiation-and-immunosuppressive-function-by-targeting-socs3-in-mice-with-b-cell-lymphoma
#19
Zhen Xu, Jianjian Ji, Jingjing Xu, Dan Li, Guoping Shi, Fei Liu, Liang Ding, Jing Ren, Huan Dou, Tingting Wang, Yayi Hou
Myeloid-derived suppressor cells (MDSCs), including granulocytic (G)-MDSCs and monocytic (M)-MDSCs, play a critical role in tumor-induced T cell tolerance. MDSCs immunosuppressive function and differentiation are significantly promoted in patients and B-cell lymphoma model mice. However, the mechanisms regulating these processes remain largely unclear. In this study, we observed increased microRNA (miR)-30a expression both in G-MDSCs and in M-MDSCs from B cell lymphoma model mice. After transfection with miR-30a mimics, the differentiation and suppressive capacities of MDSCs were significantly increased via up-regulation of arginase-1...
June 12, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28605118/histone-methyltransferase-1-regulates-the-encystation-process-in-the-parasite-giardia-lamblia
#20
Agostina Salusso, Natacha Zlocowski, Gonzalo Mayol, Nahuel Zamponi, Andrea S Rópolo
In eukaryotes, histone lysine methylation is associated with either active or repressed chromatin states, depending on the status of methylation. Even when the amino-terminus of Giardia lamblia histones diverges from other organisms, these regions contain lysine residues that are potential targets for methylation. When we examined the role of the histone methyltransferase 1 (HMT1) in the regulation of the encystation process by GlHMT1 overexpression or downregulation, we observed an increase or a decrease of cyst production respectively, compared to wild-type trophozoites...
June 12, 2017: FEBS Journal
journal
journal
40797
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"