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FEBS Journal

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https://www.readbyqxmd.com/read/29791979/functional-and-structural-characterization-of-zebrafish-asc
#1
Yajuan Li, Yi Huang, Xiaocong Cao, Xueying Yin, Xiangyu Jin, Sheng Liu, Jiansheng Jiang, Wei Jiang, Tsan Sam Xiao, Rongbin Zhou, Gang Cai, Bing Hu, Tengchuan Jin
The zebrafish genome encodes homologs for most of the proteins involved in inflammatory pathways; however, the molecular components and activation mechanisms of fish inflammasomes are largely unknown. ASC (apoptosis-associated speck-like protein containing a caspase-recruitment domain (CARD)) is the only adaptor involved in the formation of multiple types of inflammasomes. Here, we demonstrate that zASC is also involved in inflammasome activation in zebrafish. When overexpressed in vitro and in vivo in zebrafish, both the zASC and zASC pyrin domain (PYD) proteins form speck and filament structures...
May 23, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29791777/counting-on-death-quantitative-aspects-of-bcl-2-family-regulation
#2
REVIEW
Annika Hantusch, Markus Rehm, Thomas Brunner
The Bcl-2 protein family members critically regulate mitochondrial outer membrane permeabilization (MOMP), a point-of-no-return in the intrinsic and extrinsic apoptosis pathways. The common view on qualitative interaction and activation patterns of the three subclasses, the BH3-only proteins, prosurvivals and effectors, is static and currently being revolutionized by an emerging understanding of the complex dynamic equilibria that govern cellular fate. Recent experimental evidence on protein associations with the mitochondrial outer membrane, retrotranslocation to the cytosol and differential binding affinities in aqueous and membranous environments instigate the development of a revised model of Bcl-2 family interplay...
May 23, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29791776/polymorphism-in-disease-related-apolipoprotein-c-ii-amyloid-fibrils-a-structural-model-for-rod-like-fibrils
#3
Courtney O Zlatic, Yu Mao, Nevena Todorova, Yee-Foong Mok, Geoffrey J Howlett, Irene Yarovsky, Paul R Gooley, Michael D W Griffin
Human apolipoprotein (apo) C-II is one of several plasma apolipoproteins that form amyloid deposits in vivo and is an independent risk factor for cardiovascular disease. Lipid-free apoC-II readily self-assembles into twisted-ribbon amyloid fibrils but forms straight, rod-like amyloid fibrils in the presence of low concentrations of micellar phospholipids. Charge mutations exerted significantly different effects on rod-like fibril formation compared to their effects on twisted-ribbon fibril formation. For instance, the double mutant K30D, D69K apoC-II readily formed twisted-ribbon fibrils, while the rate of rod-like fibril formation in the presence of micellar phospholipid was negligible...
May 23, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29791079/overexpression-of-tet3-in-donor-cells-enhances-goat-somatic-cell-nuclear-transfer-efficiency
#4
Chengquan Han, Ruizhi Deng, Tingchao Mao, Yan Luo, Biao Wei, Peng Meng, Lu Zhao, Qing Zhang, Fusheng Quan, Liu Jun, Yong Zhang
Ten-eleven translocation 3 (TET3) mediates active DNA demethylation of paternal genomes during mouse embryonic development. However, the mechanism of DNA demethylation in goat embryos remains unknown. In addition, aberrant DNA methylation reprogramming prevalently occurs in cloned embryos by somatic cell nuclear transfer (SCNT). In this study, we reported that TET3 is a key factor in DNA demethylation in goat pre-implantation embryos. Knockdown of Tet3 hindered DNA demethylation at 2-4-cell stage in goat embryos and decreased Nanog expression in blastocysts...
May 23, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29782686/promotion-and-inhibition-of-synchronous-glycolytic-oscillations-in-yeast-by-chitosan
#5
Kenichi Shibata, Takashi Amemiya, Yu Kawakita, Kohei Obase, Kiminori Itoh, Masahiro Takinoue, Satoshi Nakata, Tomohiko Yamaguchi
Synchronous rhythmic activities play crucial roles in diverse biological systems. Glycolytic oscillations in yeast cells have been studied for 50 years with the aim of elucidating the mechanisms underlying the intracellular oscillations and their synchronization. We investigated the effects of chemical disturbances on the individual and collective glycolytic oscillations in yeast cells encapsulated in alginate microparticles, and demonstrated that the addition of chitosan, an antimicrobial agent, decreased the duration of these oscillations...
May 21, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29777624/characterisation-of-a-putative-glutamate-5-kinase-from-leishmania-donovani
#6
Natasha Sienkiewicz, Han B Ong, Alan H Fairlamb
Previous metabolic studies have demonstrated that leishmania parasites are able to synthesise proline from glutamic acid and threonine from aspartic acid. The first committed step in both biosynthetic pathways involves an amino acid kinase, either a glutamate 5-kinase (EC 2.7.2.11) or an aspartokinase (EC 2.7.2.4). Bioinformatic analysis of multiple leishmania genomes identifies a single amino-acid-kinase gene (LdBPK 262740.1) variously annotated as either a putative glutamate or aspartate kinase. To establish the catalytic function of this Leishmania donovani gene product, we have determined the physical and kinetic properties of the recombinant enzyme purified from Escherichia coli...
May 19, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29776009/dependence-receptors-the-dark-side-awakens
#7
REVIEW
Ana-Maria Negulescu, Patrick Mehlen
Transmembrane receptors are usually seen as on and off switch: when the specific ligand is bound, the receptor is on and transduces a downstream signal, while when the ligand is absent, the receptor is off. Over the last two decades several reports have argued from an alternative view where some receptors, depending on the context, will be active both in the presence and in the absence of ligand, being sort of onA and onB switch rather than on and off. These receptors have been named Dependence Receptors (DR) and they share the ability to actively trigger cell death when unbound by their respective ligands...
May 18, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29775998/high-pressure-raman-study-of-type-i-collagen
#8
Amalia Maria Paschou, Maria Katsikini, Dimitrios Christofilos, John Arvanitidis, Sotirios Ves
The high pressure response of type-I collagen from bovine Achilles tendon is investigated with micro-Raman spectroscopy. Fluorinert™ and methanol-ethanol mixtures were used as pressure transmitting media (PTM) in a diamond anvil cell. The Raman spectrum of collagen is dominated by three bands centred at approximately 1450, 1660 and 2930 cm-1 , attributed to C-H deformation, C=O stretching of the peptide bond (amide-I band) and C-H stretching modes, respectively. Upon pressure increase, using Fluorinert™ as PTM, a shift towards higher frequencies of the C-H stretching and deformation peaks is observed...
May 18, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29775996/interplay-between-adenylate-metabolizing-enzymes-and-amp-activated-protein-kinase
#9
REVIEW
Marcella Camici, Simone Allegrini, Maria Grazia Tozzi
Purine nucleotides are involved in a variety of cellular functions, such as energy storage and transfer, and signalling, in addition to being the precursors of nucleic acids and cofactors of many biochemical reactions. They can be generated through two separate pathways, the de novo biosynthesis pathway and the salvage pathway. De novo purine biosynthesis leads to the formation of IMP, from which the adenylate and guanylate pools are generated by two additional steps. The salvage pathways utilize hypoxanthine, guanine and adenine to generate the corresponding mononucleotides...
May 18, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29775995/phosphorylation-alters-bim-mediated-mcl-1-stabilization-and-priming
#10
Jason E Conage-Pough, Lawrence H Boise
Mcl-1 is a highly labile protein, subject to extensive posttranslational regulation. This distinguishes Mcl-1 from other anti-apoptotic proteins and necessitates further study to better understand how interactions with pro-apoptotic Bcl-2 proteins affect its regulation. One such protein, Bim, is known to stabilize Mcl-1, and Bim phosphorylation has been associated with increased Mcl-1 binding. Consequently, we investigated the potential impact of Bim phosphorylation on Mcl-1 stability. We found that Bim stabilizes and primes Mcl-1 in RPCI-WM1 cells and is constitutively phosphorylated...
May 18, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29775245/subunits-of-the-dna-polymerase-alpha-primase-complex-promote-notch-mediated-proliferation-with-discrete-and-shared-functions-in-c-elegans-germline
#11
Dong Suk Yoon, Dong Seok Cha, Mohammad A Alfhili, Brett D Keiper, Myon-Hee Lee
Notch receptor signaling is a highly conserved cell communication system in most multicellular organisms and plays a critical role at several junctures in animal development. In C. elegans, GLP-1/Notch signaling is essential for both germline stem cell maintenance and germ cell proliferation during gonad development. Here, we show that subunits (POLA-1, DIV-1, PRI-1, and PRI-2) of the DNA polymerase alpha-primase complex are required for germ cell proliferation in response to GLP-1/Notch signaling in different tissues at different developmental stages...
May 18, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29775242/mutations-in-mars-identified-in-a-specific-type-of-pulmonary-alveolar-proteinosis-alter-methionyl-trna-synthetase-activity
#12
Martine Comisso, Alice Hadchouel, Jacques de Blic, Marc Mirande
Biallelic missense mutations in MARS are responsible for rare but severe cases of pulmonary alveolar proteinosis (PAP) prevalent on the island of La Réunion. MARS encodes cytosolic methionyl-tRNA synthetase (MetRS), an essential translation factor. The multisystemic effects observed in patients with this form of PAP is consistent with a loss-of-function defect in an ubiquitously expressed enzyme. The pathophysiological mechanisms involved in MARS related PAP are currently unknown. In this work, we analyzed the effect of the PAP related mutations in MARS on the thermal stability and on the catalytic parameters of the MetRS mutants, relative to wild-type...
May 18, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29775232/mannosylinositol-phosphorylceramides-and-ergosterol-coodinately-maintain-cell-wall-integrity-in-the-yeast-saccharomyces-cerevisiae
#13
Seiya Tanaka, Motohiro Tani
In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of the normal composition causes several cellular dysfunctions. Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity. In this study, we screened for multicopy suppressor genes that rescue the defect in cell wall integrity in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis...
May 18, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29770564/physiological-and-oncogenic-roles-of-the-prl-phosphatases
#14
REVIEW
Serge Hardy, Elie Kostantin, Teri Hatzihristidis, Yevgen Zolotarov, Noriko Uetani, Michel L Tremblay
The human Phosphatase of Regenerative Liver (PRL) family comprises three members (PRL-1, -2, -3; gene name PTP4A1, PTP4A2, PTP4A3) that are highly expressed in a majority of cancers. This review summarizes our current understanding of PRL biology, including an overview of their evolutionary relationships and the regulatory mechanisms controlling their expression. We provide an updated view on our current knowledge on the PRL functions in solid tumors, hematological cancer and normal physiology, particularly emphasizing on the use of in vivo mouse models...
May 16, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29766642/arf1-and-er-mitochondrial-tethering-a-new-trick-for-an-old-dog
#15
Dana A Davis
Endoplasmic reticulum (ER)-mitochondria tethering by the ERMES complex is critical for numerous mitochondrial functions in fungi. It was recently discovered that the GTPase Arf1, which regulates within Golgi vesicular trafficking also affects ER-mitochondrial tethering potentially through ERMES. In Candida albicans, Arf1 not only functions in vesicular trafficking and affects ERMES but also impacts reactive oxygen species generation, which is a key immune defense molecule.
May 15, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29761627/muscle-fiber-type-diversity-revealed-by-anti-myosin-heavy-chain-antibodies
#16
REVIEW
Stefano Schiaffino
Different forms of myosin heavy chains (MyHCs), coded by a large family of sarcomeric MYH genes, are expressed in striated muscles. The generation of specific anti-MyHC antibodies has provided a powerful tool to define the fiber types present in skeletal muscles, their functional properties, their response to conditions that affect muscle plasticity and their changes in muscle disorders. Cardiomyocyte heterogeneity has been revealed by the serendipitous observation that different MyHCs are present in atrial and ventricular myocardium and in heart conduction tissue...
May 14, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29756681/neuronal-il-4r%C3%AE-modulates-neuronal-apoptosis-and-cell-viability-during-the-acute-phases-of-cerebral-ischemia
#17
Han Kyu Lee, Sehwon Koh, Donald C Lo, Douglas A Marchuk
Ischemic stroke caused by an embolus or local thrombosis results in neural tissue damage (an infarct) in the territory of the occluded cerebral artery. Decades of studies have increased our understanding of the molecular events during cerebral infarction; however, translation of these discoveries to druggable targets for ischemic stroke treatment has been largely disappointing. Interleukin-4 (IL-4) is a multifunctional cytokine that exerts its cellular activities via the interleukin-4 receptor α (IL-4Rα)...
May 14, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29745002/helicobacter-pylori-shows-asymmetric-and-polar-cell-divisome-assembly-associated-with-dna-replisome
#18
Mohammad Kamran, Priyanka Dubey, Vijay Verma, Santanu Dasgupta, Suman K Dhar
DNA replication and cell division are two fundamental processes in the life cycle of a cell. Majority of the prokaryotic cells undergo division by means of binary fission in coordination with replication of the genome. Both processes, specially their coordination, are poorly understood in Helicobacter pylori. Here, we studied the cell divisome assembly and the subsequent processes of membrane and peptidoglycan syntheses in the bacterium. To our surprise, we found the cell divisome assembly to be polar, as well-corroborated by the asymmetric membrane and peptidoglycan synthesis at the poles...
May 9, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29734522/a-selective-determination-of-the-catalytic-cysteine-pk-a-of-2-cysteine-succinic-semialdehyde-dehydrogenase-from-acinetobacter-baumannii-using-burst-kinetics-and-enzyme-adduct-formation
#19
Jittima Phonbuppha, Somchart Maenpuen, Pobthum Munkajohnpong, Pimchai Chaiyen, Ruchanok Tinikul
Succinic semialdehyde dehydrogenase from Acinetobacter baumannii (AbSSADH) catalyzes the oxidation of succinic semialdehyde. This enzyme has two conserved cysteines (Cys289 and Cys291) and prefers to use NADP+ over NAD+ as a hydride acceptor. Steady-state kinetic analysis showed that AbSSADH has the highest catalytic turnover (137 s-1 ) and can tolerate SSA inhibition the most (<500 μM) among all SSADHs reported. Alanine substitutions of the two conserved cysteines indicated that Cys291Ala has ~65% activity compared to the wild-type enzyme while Cys289Ala is inactive, suggesting that Cys289 is the active residue participating in catalysis...
May 7, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29734505/identification-of-phosphorylation-sites-and-binding-pockets-for-modulation-of-na-v-1-5-channel-by-fyn-tyrosine-kinase
#20
Shahid Muhammad Iqbal, Mohammed Aufy, Waheed Shabbir, Rosa Lemmens-Gruber
Cardiac sodium channel NaV 1.5 is the predominant form of sodium channels in cardiomyocytes, which exists as a macromolecular complex and interacts with multiple protein partners. Fyn kinase is one of the interacting proteins which co-localize, phosphorylate and modulate the NaV 1.5 channel. To elaborate this interaction we created expression vectors for the N-terminal, intracellular loop and C-terminal regions of the NaV 1.5 channel, to express in HEK-293 cells. By co-immunoprecipitation and anti-phosphotyrosine blotting, we identified proline-rich binding sites for Fyn kinase in the N-terminal, IC-loopi-ii and C-terminal...
May 7, 2018: FEBS Journal
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