journal
https://read.qxmd.com/read/38000010/comparative-transcriptome-analysis-reveals-the-molecular-mechanisms-of-acetic-acid-reduction-by-adding-nahso-3-in-actinobacillus-succinogenes-gxas137
#21
JOURNAL ARTICLE
Shiyong Li, Chaodong Song, Hongyan Zhang, Yan Qin, Mingguo Jiang, Naikun Shen
Acetic acid (AC) is a major by-product from fermentation processes for producing succinic acid (SA) using Actinobacillus succinogenes . Previous experiments have demonstrated that sodium bisulfate (NaHSO3 ) can significantly decrease AC production by A. succinogenes GXAS137 during SA fermentation. However, the mechanism of AC reduction is poorly understood. In this study, the transcriptional profiles of the strain were compared through Illumina RNA-seq to identify differentially expressed genes (DEGs). A total of 210 DEGs were identified by expression analysis: 83 and 127 genes up-regulated and down-regulated, respectively, in response to NaHSO3 treatment...
November 24, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37999986/comparison-of-mcp-and-tsc-media-to-enumerate-clostridium-perfringens-in-surface-water-samples
#22
JOURNAL ARTICLE
Renata Matuszewska, Łukasz Mąka
The Clostridium perfringens bacteria are used to assess water quality as an indicator parameter. If detected, it can confirm the occurrence of past fecal contamination. Tests determining C. perfringens in water samples are usually performed by membrane filtration where filters are incubated on selective media under anaerobic conditions. Available media include mCP and TSC. The aim of this study was to compare the relative recovery of C. perfringens (including spores) from surface water samples and to determine the performance characteristics of the membrane filtration method using both media...
November 24, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37934050/evaluating-the-sensitivity-of-different-molecular-techniques-for-detecting-mycobacterium-tuberculosis-complex-in-patients-with-pulmonary-infection
#23
JOURNAL ARTICLE
Hassan A Hemeg, Hamzah O Albulushi, Hani A Ozbak, Hamza M Ali, Emad K Alahmadi, Yahya A Almutawif, Sari T Alhuofie, Rana A Alaeq, Areej A Alhazmi, Mustafa A Najim, Ahmed M Hanafy
This study aimed to evaluate the accuracy of detecting drug-resistant Mycobacterium tuberculosis complex (MTBC)-specific DNA in sputum specimens from 48 patients diagnosed with pulmonary tuberculosis. The presence of MTBC DNA in the specimens was validated using the GeneXpert MTB/RIF system and compared with a specific PCR assay targeting the IS 6110 and the mtp 40 gene sequence fragments. Additionally, the results obtained by multiplex PCR assays to detect the most frequently encountered rifampin, isoniazid, and ethambutol resistance-conferring mutations were matched with those obtained by GeneXpert and phenotypic culture-based drug susceptibility tests...
November 6, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37875068/a-mini-review-of-enteroaggregative-escherichia-coli-with-a-specific-target-on-the-virulence-factors-controlled-by-the-aggr-master-regulator
#24
JOURNAL ARTICLE
Jeannett Alejandra Izquierdo-Vega, Rubi Joseline Castillo-Juarez, Manuel Sánchez-Gutiérrez, Miguel A Ares, Miguel A De La Cruz
Enteroaggregative Escherichia coli (EAEC) strains have been linked to several outbreaks of severe diarrhea around the world, and this bacterium is now commonly resistant to antibiotics. As part of the pathophysiology of EAEC, the characteristic pattern of adherence looks like stacked bricks on the intestinal epithelium. This phenotype depends on an aggregative adhesion plasmid (pAA), which codes for a regulatory protein named AggR. The AggR protein is a master regulator that transcriptionally actives the main virulence genes in this E...
October 24, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37817297/biological-characteristics-of-beauveria-majiangensis-strain-mj1015-and-optimization-of-solid-medium-technology-for-sporulation
#25
JOURNAL ARTICLE
Xinghong Wang, Zhenxing Huang, Congchun Li, Man Liu, Guangming Yang, Lilin Luo, Yongchao Rao, Yan Shen, Jing Wang
The entomopathogenic fungus Beauveria majiangensis strain MJ1015, recently isolated from white grubs on a blueberry farm in Guizhou, China, could be used as a biocontrol agent. As a first step toward determining the effect of different solid culture media, temperature, and pH on colony growth rate and sporulation, we evaluated the optimum solid medium for mycelial growth and conidia production on a commercial scale. Subsequently, we also used single-factor analysis and response surface optimization to optimize the composition of the solid culture medium...
October 10, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37816501/do-nsaids-and-other-pain-relief-drugs-can-inhibit-the-growth-of-lactobacillaceae
#26
JOURNAL ARTICLE
Hanna Kruszewska, Anna Zawistowska-Rojek, Stefan Tyski
Non-steroidal anti-inflammatory drugs (NSAIDs) commonly used in clinical practice may cause gastrointestinal injuries and influence the gut microbiota. This study investigated the effects of various NSAIDs and some analgesics on the viability of Lactobacillaceae strains (including probiotic strains) in vitro . It was found that diclofenac, ibuprofen, ketoprofen, dexketoprofen, flurbiprofen, and acetylsalicylic acid inhibited the growth of lactobacilli at a concentration of 0.05-3.2 mg/ml. These MICs of NSAIDs are well above therapeutic plasma concentrations achieved in humans, indicating that the tested drugs should not inhibit the growth of lactobacilli in the human digestive tract...
October 10, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37668434/seroepidemiological-survey-of-cytomegalovirus-infection-among-pregnant-women-in-sudan
#27
JOURNAL ARTICLE
Ehssan H Moglad, Ahmed O Hassan, Mawada S Atta Elmanan, Samar M Saeed, Wafaa Mohammed Abdalla, Kawthar Abdelgaleil Mohammedsalih, Hatim T Ali, Mohammed Siddig Abd Elaziz, Hind Haidar Ahmed
Human Cytomegalovirus (HCMV) is a leading healthcare problem associated with stillbirth and congenital abnormalities. Determining the seroprevalence and the possible risk factors related to HCMV infections may be a cornerstone in preventing its complications. This cross-sectional study was conducted in Kassala and River Nile States to determine the seroprevalence and risk factors associated with HCMV infection in pregnant women. One hundred eighty-four (n = 184) blood specimens were collected from pregnant women from February 2018 to January 2020...
September 5, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37725900/cloning-heterologous-expression-and-characterization-of-a-neutral-uricase-from-arthrobacter-sp-csaj-16-in-cangshan-mountain
#28
JOURNAL ARTICLE
Xin Yan, Wei Hu, Yun-Guo Zhu, Qing-Qing Liu, Shuai Wang, Hong-Yan Liu, Dan Zhu, Zhi-Hua Lv, Lin-Hua Li, Yi-Rui Yin
Uricase (or Urate oxidase), a key enzyme involved in purine metabolism, is commonly used in treating conditions such as gout, hyperuricemia, and tumor lysis syndrome. In this study, a uricase-producing strain (named CSAJ-16) was isolated from the soil sample of Cangshan Mountain, Yunnan Province, China. This strain was identified as Arthrobacter sp. CSAJ-16. Based on the gene sequence alignment, the uricase gene (named aruox ) of Arthrobacter sp. CSAJ-16 was amplified and heterologously expressed. The recombinant uricase (ArUOX) was about 32 kDa...
September 1, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37725899/identification-of-hub-genes-and-typing-of-tuberculosis-infections-based-on-autophagy-related-genes
#29
JOURNAL ARTICLE
Yunfeng Sheng, Haibo Hua, Yan Yong, Lihong Zhou
Tuberculosis (TB) caused by Mycobacterium tuberculosis is one of the leading causes of morbidity and death in humans worldwide. Some autophagy genes associated with TB and some miRNAs regulating TB have been found, but the identification of autophagy-related genes in M. tuberculosis remains to be explored. Forty-seven autophagy-related genes differentially expressed in TB were identified in this study by analysis of TB-related datasets in the Gene Expression Omnibus (GEO) and autophagy-related genes in the Human Autophagy Database...
September 1, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37725898/control-effect-and-mechanism-of-trichoderma-asperellum-tm11-against-blueberry-root-rot
#30
JOURNAL ARTICLE
Si Li, Fu-Mei Zhang, Xiao-Jing Shang, Rui Hou
Fusarium oxysporum is the primary pathogen of blueberry root rot; furthermore, we found that Fusarium commune can also cause root rot in blueberries. Trichoderma spp. is widely used to control plant diseases. We isolated Trichoderma asperellum (TM11) from blueberry rhizosphere soil to explore its control effect and mechanism on F. oxysporum and F. commune . We found that the inhibitory effects of TM11 volatiles and broth metabolites on F. oxysporum were significant, but only F. commune volatile metabolites had a significant inhibitory effect on its growth...
September 1, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37725897/kestose-increases-the-relative-abundance-of-faecalibacterium-spp-and-nominally-increases-cow-milk-tolerant-dose-in-children-with-cow-s-milk-allergy-preliminary-results
#31
JOURNAL ARTICLE
Shohei Kubota, Shiro Sugiura, Mayuko Takahashi, Yoshihiro Kadota, Yoshihiro Takasato, Teruaki Matsui, Katsumasa Kitamura, Takumi Tochio, Komei Ito
A single-arm study was conducted with 10 children aged 2-12 years with severe cow's milk allergy (CMA) requiring complete allergen elimination. Subjects were administered kestose, a prebiotic, at 1 or 2 g/day for 12 weeks. Results of a subsequent oral food challenge (OFC) showed a statistically significant increase in the total dose of cow's milk ingestion (1.6 ml vs. 2.7 ml, p = 0.041). However, the overall evaluation of the OFC results, TS/Pro (total score of Anaphylaxis Scoring Aichi (ASCA)/cumulative dose of protein), showed no statistically significant improvement, although the values were nominally improved in seven out of 10 subjects...
September 1, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37725896/screening-of-potential-probiotic-lactobacillaceae-and-their-improvement-of-type-2-diabetes-mellitus-by-promoting-pi3k-akt-signaling-pathway-in-db-db-mice
#32
JOURNAL ARTICLE
Yueyang Li, Tong Tong, Peifan Li, Yian Peng, Michael Zhang, Jia Liu, Yongxin She, Zuming Li, Yongli Li
The study aimed to isolate Lactobacillaceae strains with in vitro hypoglycemic activity and probiotic properties and to determine their antidiabetic abilities in vivo . Lactiplantibacillus plantarum 22, L. plantarum 25, Limosilactobacillus fermentum 11, and L. fermentum 305 with high in vitro hypoglycemic activity were screened from 23 strains of Lactobacillaceae isolated from human feces and identified by 16S rDNA sequencing. The fasting blood glucose (FBG) of the mice was recorded weekly. After 12 weeks, liver, kidney, and pancreas tissues were stained with hematoxylin and eosin (H&E) to observe histomorphology; the inflammatory factors were assayed by Quantitative Real-time PCR; PI3K and AKT were measured by Western blot; the short-chain fatty acids (SCFAs) were determined by LC-MS/MS...
September 1, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37725895/a-recombinase-aided-amplification-assay-for-the-detection-of-chlamydia-felis
#33
JOURNAL ARTICLE
Jian Liu, Weidong Qian, Jian Wang, Yilan Bai, Yaping Gui, Luming Xia, Guohua Gong, Feifei Ge, Haixiao Shen, Xiaojing Chang, Hongjin Zhao
Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis . RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the C. felis plasmid DNA per reaction. No positive signals for other pathogens were detected. The coincidence rate of RAA and conventional PCR was 95.24% (20/21) and 100% (96/96) for positive and negative samples, respectively. The established RAA assay is a simple, rapid, highly sensitive, and specific method for detecting C...
September 1, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37725894/dissemination-and-characterization-of-stenotrophomonas-maltophilia-isolates-from-dairy-cows-in-northeast-china
#34
JOURNAL ARTICLE
Lanjun Shao, Xiaojing Liu, Yi Liu, Jie Shen, Ruishan Liu, Pingnan Chen
This work investigated the genetic relationship among Stenotrophomonas maltophilia strains in fecal samples from dairy cows in northeast China and identified the dominant β-lactamase genotype. One hundred and six samples were collected from two randomly selected cow farms in northeast China, and the isolates were identified with MALDI-TOF/MS. Whole-genome sequencing was conducted using Illumina HiSeq 4000-PE150 platform (Illumina, Inc., USA). The antimicrobial resistance genes were detected using CGE services...
September 1, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37725893/the-prokaryotic-microalga-limnothrix-redekei-knua012-to-improve-aldehyde-decarbonylase-expression-for-use-as-a-biological-resource
#35
JOURNAL ARTICLE
Young-Saeng Kim, Haeri Baek, Hyun-Sik Yun, Jae-Hak Lee, Kyoung-In Lee, Han-Soon Kim, Ho-Sung Yoon
The prokaryotic microalga Limnothrix redekei KNUA012 isolated from a freshwater bloom sample from Lake Hapcheon, Hapcheon-gun, South Korea, was investigated for its potential as a biofuel feedstock. Microalgae produce straight-chain alkanes/alkenes from acyl carrier protein-linked fatty acyls via aldehyde decarbonylase (AD; EC 1.2.1.3), which can convert aldehyde intermediates into various biofuel precursors, such as alkanes and free fatty acids. In L. redekei KNUA012, long-chain ADs can convert fatty aldehyde intermediates into alkanes...
September 1, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37725892/the-impacts-of-fecal-microbiota-transplantation-from-same-sex-on-the-symptoms-of-ulcerative-colitis-patients
#36
JOURNAL ARTICLE
Shu Shang, Jian Zhu, Xi Liu, Wei Wang, Tingting Dai, Li Wang, Baojun Li
We aimed to compare the clinical efficacy of fecal microbiota transplantation (FMT) from the same sex on ulcerative colitis (UC) patients. A total of 272 UC patients were selected in the prospective clinical study, which incorporated four distinct groups, each comprising male and female patients, who were either receiving FMT or placebo, respectively. FMT was performed by sending the gut microbiota of healthy female or male adolescents to the same gender patients via gastroscope three times (one time/three weeks), and a placebo was used with an equal volume of saline...
September 1, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37606269/comparative-study-of-linezolid-and-vancomycin-regimens-in-one-stage-surgery-for-treating-limb-traumatic-osteomyelitis-caused-by-methicillin-resistant-staphylococcus-aureus
#37
JOURNAL ARTICLE
Rongchang Zhou, Kai Huang, Qiaofeng Guo, Bingyuan Lin, Haiyong Ren, Yiyang Liu, Hongu Song
To compare the clinical outcomes of linezolid and vancomycin regimens combined with one-stage surgery in treating traumatic osteomyelitis of the limbs caused by methicillin-resistant Staphylococcus aureus (MRSA) infection. A retrospective study was performed to analyze patients with traumatic osteomyelitis of the limbs attributable to MRSA infection. All of these patients received one-stage surgery to debride their wounds, with subsequent implantation of a vancomycin-loaded calcium sulfate artificial bone. Patients received either intravenous linezolid (study group) or vancomycin (control group) during the perioperative period...
August 22, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37314360/transfer-of-t6ss-spi-19-from-salmonella-gallinarum-to-salmonella-typhimurium-lacking-t6ss-spi-6-complements-its-colonization-defect-in-mice
#38
JOURNAL ARTICLE
David Pezoa, Carlos J Blondel, Fernando A Amaya, Carlos A Santiviago
Salmonella genus harbors five Type VI Secretion System (T6SS) gene clusters. The T6SS encoded in SPI-6 (T6SSSPI-6 ) contributes to Salmonella Typhimurium colonization of chickens and mice, while the T6SS encoded in SPI-19 (T6SSSPI-19 ) of Salmonella Gallinarum contributes to chicken colonization. Interestingly, the T6SSSPI-19 of Salmonella Gallinarum complemented the defect in chicken colonization of a Salmonella Typhimurium strain that lacks the T6SSSPI-6 , suggesting that both T6SSs are interchangeable. Here we show that the transfer of Salmonella Gallinarum T6SSSPI-19 complemented the defect in mice colonization of a Salmonella Typhimurium ΔT6SSSPI-6 strain, indicating that both T6SSs are functionally redundant during host colonization...
June 1, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37314359/contribution-of-ypro15c-overexpression-to-the-resistance-of-saccharomyces-cerevisiae-by4742-strain-to-furfural-inhibitor
#39
JOURNAL ARTICLE
Getachew Tafere Abrha, Qian Li, Xiaolin Kuang, Difan Xiao, Ellen Ayepa, Jinjian Wu, Huan Chen, Zhengyue Zhang, Yina Liu, Xiumei Yu, Quanju Xiang, Menggen Ma
Lignocellulosic biomass is still considered a feasible source of bioethanol production. Saccharomyces cerevisiae can adapt to detoxify lignocellulose-derived inhibitors, including furfural. Tolerance of strain performance has been measured by the extent of the lag phase for cell proliferation following the furfural inhibitor challenge. The purpose of this work was to obtain a tolerant yeast strain against furfural through overexpression of YPR015C using the in vivo homologous recombination method. The physiological observation of the overexpressing yeast strain showed that it was more resistant to furfural than its parental strain...
June 1, 2023: Polish Journal of Microbiology
https://read.qxmd.com/read/37314358/physicochemical-and-microbiological-water-quality-assessment-of-a-northwestern-algerian-dam-detection-of-ichtyopathogenic-bacteria
#40
JOURNAL ARTICLE
Hanane Boumerdassi, Lydia Neïla Djouadi, Abderrahmane Hambli, Marie-Laure Fardeau, Hadda-Imen Ouzari, Farida Nateche
Freshwater fish are often exposed to threats from anthropogenic or natural origins, such as pathogenic or opportunistic microorganisms responsible for a broad range of severe infections. In this study, we aimed to assess this microbiological threat to fish in an Algerian northwestern dam Sekkak (Tlemcen) by evaluating the diversity of ichtyopathogenic bacteria. In order to determine the water quality, physicochemical analyses of the dam water were carried out in situ. Ichtyopathogenic bacteria were isolated on selective media and identified by API galleries and molecular techniques (PCR and sequencing of the 16S rRNA gene)...
June 1, 2023: Polish Journal of Microbiology
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