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Polish Journal of Microbiology

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https://www.readbyqxmd.com/read/28520333/monitoring-of-virulence-genes-drug-resistance-in-campylobacter-coli-isolated-from-golden-retrievers
#1
Marek Selwet, Mariola Galbas, Ryszard Słomski, Tomasz Cłapa, Filip Porzucek
The investigation was performed on 75 of Golden Retriever puppies. Faecal samples were collected on the 42 day of the puppies life (control). Probiotic preparation was administered on 43 day of the puppies life and 10 days after the application of the probiotic, faecal samples were collected again (on 53 day of puppies life). All isolates of Campylobacter coli isolated prior to the administration of the probiotic were found to contain the cadF gene responsible for adhesion, as well as, the flaA gene influencing motility of the examined bacteria...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28520332/enteroviruses-associated-with-aseptic-meningitis-in-poland-2011%C3%A2-2014
#2
Magdalena Wieczorek, Agnieszka Figas, Arleta Krzysztoszek
A 4-year study (2011–2014) of patients with meningitis was performed. Out of the 686 cerebrospinal fluid samples, 465 (67.8%) were positive for eneteroviruses using RT-PCR and out of 334 clinical samples, 216 (64.7%) were positive for enteroviruses using cell culture methods. The highest detection rate was observed in the summer and autumn. In total, 185 enteroviruses were identified by using neutralization test. Echovirus 6 and 30 were the most common (41.7% and 37.5% respectively). The highest frequency of neurological infections (32...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28520331/cytotoxic-and-bacteriostatic-activity-of-nanostructured-tio-2-coatings
#3
Alessandro Di Cerbo, Federica Pezzuto, Antonio Scarano
Nanostructures are structures, mainly synthetic (nanosurfaces, cylindrical nanotubes, and nanospheres), which range between 1–100 nm in at least one dimension and can be engineered to a wide range of physical properties. This paper aims to explore the bacteriostatic and cytotoxic characteristics of nano-TiO(2) coated specimens of glass, stainless steel and ceramic with different thickness and roughness. The results show that stainless steel and glass specimens with a nano-TiO(2) coating thickness of 200 nm have a bacteriostatic effect of 97% and 100%, respectively after 30 minutes of UV exposure...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28520330/qualitative-and-quantitative-characteristics-of-selected-bacterial-groups-in-children-with-inflammatory-bowel-diseases
#4
Dorota Roszak, Mirosława Gałęcka, Wojciech Cichy, Patrycja Szachta
The aim of the study was evaluation of qualitative and quantitative changes in bacterial ecosystem in 109 children with inflammatory bowel diseases. Stools obtained from patients were analysed for selected bacteria and concentration of faecal inflammatory markers (calprotectin, lactoferrin, M2-PK). The number of selected microorganisms depends on the level of clinical activity of disease and is correlated with faecal concentration of inflammatory markers. Differences in microflora disturbance, observed in patients with Crohn’s disease and ulcerative colitis, may suggest different causes of development of both pathologies...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28520329/characterization-of-staphylococcal-cassette-chromosome-mec-sccmec-in-methicillin-resistant-staphylococcus-epidermidis-strains-isolated-from-biomaterial-associated-infections-and-their-antibiotic-resistance-patterns
#5
Ewa Szczuka, Karolina Bosacka, Adam Kaznowski
This work aims to provide an insight into staphylococcal cassette chromosome mec elements and antibiotic resistance in clinical isolates of Staphylococcus epidermidis. The dominating type was SCCmec – IV. Fifteen isolates were assigned to SCCmec type III, two isolates to SCCmec type II. Most isolates were resistant to at least three of the non-β-lactam antibiotics tested. None of the strains exhibited resistance to new generation antibiotics, such as daptomycin and linezolid. Also, none of these strains showed resistance to tigecycline and only four strains were resistant to rifampin i...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28520328/identification-and-localization-of-%C3%AE-d-glucosidase-from-two-typical-oenococcus-oeni-strains
#6
Yahui Li, Yanhong Ma, Kaihong Huang, Hongzhi Zhang
β-D-glucosidase (βG) gene from Oenococcus oeni SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further localized. The results showed that βG gene of the two strains was in high homology (> 99%) to reported βG gene, confirming both strains possess βG activity at the molecular level. Intracellular βG of SD-2a is a mainly soluble protein, existing mostly in the cytoplasm and to some extent in the periplasm. While for 31MBR, intracellular βG is mainly insoluble protein existing in the cytoplasmic membrane...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28517923/prevalence-of-parasitic-contamination-in-salad-vegetables-collected-from-supermarkets-and-street-vendors-in-amman-and-baqa%C3%A2-a-jordan
#7
Yazan Ismail
One of the main ways in transmitting parasites to humans is through consuming contaminated raw vegetables. The aim of this study was to evaluate the prevalence of parasitological contamination (helminthes eggs, Giardia and Entamoeba histolytica cysts) of salad vegetables sold at supermarkets and street vendors in Amman and Baqa’a – Jordan. A total of 133 samples of salad vegetables were collected and examined for the prevalence of parasites. It was found that 29% of the samples were contaminated with different parasites...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28517921/levels-of-organic-compounds-number-of-microorganisms-and-cadmium-accumulation-in-festuca-ovina-hydroponic-culture
#8
Małgorzata Majewska, Anna Słomka
Understanding the microbiological, biochemical and physiological aspects of phytoremediation of soil and water environments polluted to different degrees with heavy metals has very important theoretical and practical implications. In this study, a comparison was made between total cadmium concentration in root and shoot tissues as well as concentrations of particular fractions of Cd immobilized by roots of Festuca ovina (Sheep’s fescue) hydroponically cultivated in nutrient solutions supplemented with 1 μg Cd ml(–1) and those cultivated at 10 μg Cd ml(–1)...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28517920/characterization-of-bacteria-isolation-of-bacteria-from-pinyon-rhizosphere-producing-biosurfactants-from-agro-industrial-waste
#9
(no author information available yet)
Two hundred and fifty bacterial strains were isolated from pinyon rhizosphere and screened for biosurfactants production. Among them, six bacterial strains were selected for their potential to produce biosurfactants using two low cost wastes, crude glycerol and lactoserum, as raw material. Both wastes were useful for producing biosurfactants because of their high content in fat and carbohydrates. The six strains were identified by 16S rDNA with an identity percentage higher than 95%, three strains belonged to Enterobacter sp...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28517919/enzymes-involved-in-naproxen-degradation-by-planococcus-sp-s5
#10
Danuta Wojcieszyńska, Dorota Domaradzka, Katarzyna Hupert-Kocurek, Urszula Guzik
Naproxen is a one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) entering the environment as a result of high consumption. For this reason, there is an emerging need to recognize mechanisms of its degradation and enzymes engaged in this process. Planococcus sp. S5 is a gram positive strain able to degrade naproxen in monosubstrate culture (27%). However, naproxen is not a sufficient growth substrate for this strain. In the presence of benzoate, 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid or vanillic acid as growth substrates, the degradation of 21...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28517918/biocontrol-of-gray-mold-decay-in-pear-by-bacillus-amyloliquefaciens-strain-ba3-and-its-effect-on-postharvest-quality-parameters
#11
Hui Qu, Longyu Zhao, Fengchun Zhao, Yufang Liu, Zhengyou Yang
The economic losses caused by postharvest fruits diseases have attracted global attention. Traditional chemical fungicide could not meet the need of humans. In recent years, microbial agent which has begun to take the place of chemical fungicide comes into people’s vision. The aim of this paper was to investigate the potential of Bacillus amyloliquefaciens strain BA3 for its biocontrol capability on gray mold decay of pears and its effect on postharvest quality of pears. Compared with other treatments, the inhibition effect on gray mold of washed cell suspension of B...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28517917/interaction-of-gram-positive-and-gram-negative-bacteria-with-ceramic-nanomaterials-obtained-by-combustion-synthesis-%C3%A2-adsorption-and-cytotoxicity-studies
#12
Andrzej Borkowski, Filip Owczarek, Mateusz Szala, Marek Selwet
This paper presents the interactions of Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas putida) bacteria with ceramic materials obtained by combustion synthesis. These studies were conducted based on an analysis of the adsorption of bacteria onto aggregates of ceramic materials in an aqueous suspension. The materials used in the studies were of a nanostructured nature and consisted mainly of carbides: silicon carbide (SiC) in the form of nanofibers (NFs) and nanorods (NRs), titanium carbide, and graphite, which can also be formed by combustion synthesis...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28517916/relationship-between-ureb-sequence-diversity-urease-activity-and-genotypic-variations-of-different-helicobacter-pylori-strains-in-patients-with-gastric-disorders
#13
Hossein Ghalehnoei, Alireza Ahmadzadeh, Nastaran Farzi, Masoud Alebouyeh, Hamid Asadzadeh Aghdaei, Pendram Azimzadeh, Mahsa Molaei, Mohammad Reza Zali
Association of the severity of Helicobacter pylori induced diseases with virulence entity of the colonized strains was proven in some studies. Urease has been demonstrated as a potent virulence factor for H. pylori. The main aim of this study was investigation of the relationships of ureB sequence diversity, urease activity and virulence genotypes of different H. pylori strains with histopathological changes of gastric tissue in infected patients suffering from different gastric disorders. Analysis of the virulence genotypes in the isolated strains indicated significant associations between the presence of severe active gastritis and cagA+ (P = 0...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28517915/microbial-glycosylation-of-flavonoids
#14
REVIEW
Sandra Sordon, Jarosław Popłoński, Ewa Huszcza
Flavonoids constitute a large group of polyphenolic compounds naturally found in plants, which have a wide range of biological activity. Although flavonoids are beneficial to human health, their application is limited by their low bioavailability and poor water-solubility. Therefore, recently there has been a particular interest in glycosylated forms of flavonoids, which usually are better soluble, more stable, and more functional compared to their aglycones. Microbial transformation of natural flavonoids may be an attractive way of receiving their glycosylated derivatives in amounts sufficient for the research on the effect of glycoside group on compound properties and for further application of these compounds as ingredients of dietary supplements and pharmaceuticals...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/27282005/etiologic-agents-and-antifungal-susceptibility-of-oral-candidosis-from-romanian-patients-with-hiv-infection-or-type-1-diabetes-mellitus
#15
Bogdan Minea, Valentin Nastasa, Anna Kolecka, Magdalena Mares, Narcisa Marangoci, Irina Rosca, Mariana Pinteala, Monica Hancianu, Mihai Mares
This is the first Romanian investigation of oral candidosis in patients suffering of HIV-infection or type 1 diabetes mellitus (T1DM). Candida albicans was the dominant species in both types of isolates: n = 14 (46.7%) in T1DM, n = 60 (69.8%) in HIV. The most frequent non-albicans Candida spp. were Candida kefyr (n = 6; 20%) in T1DM and Candida dubliniensis (n = 8; 9.3%) in HIV. Resistance to fluconazole was detected only in the HIV non-albicans Candida group (n = 8; 9.3%). All isolates were susceptible to VOR...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/27282004/igg-avidity-an-important-serologic-marker-for-the-diagnosis-of-tick-borne-encephalitis-virus-infection
#16
Tatjana Vilibic-Cavlek, Ljubo Barbic, Vladimir Stevanovic, Goranka Petrovic, Gordana Mlinaric-Galinovic
A total of 52 serum samples from patients with symptoms suggestive of tick-borne encephalitis virus (TBEV) infection and positive IgM and/or IgG antibodies were tested for IgG avidity. Acute/recent TBEV infection was confirmed by low/borderline avidity index (AI) in 94.8% IgM positive/IgG positive samples, while in 5.2% high AI was found indicating persisting IgM antibodies. Majority of IgM negative/IgG positive samples (78.6%) showed high AI consistent with past TBEV infection. However, in 21.3% patients without measurable IgM antibodies current/recent infection was confirmed by AI...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/27282003/enterobacter-asburiae-kuni5-a-nickel-resistant-bacterium-for-possible-bioremediation-of-nickel-contaminated-sites
#17
Anirudha Paul, Samir Kumar Mukherjee
Nickel resistant bacterial strain Enterobacter asburiae KUNi5 was isolated and showed resistance up to 15 mM and could remove Ni optimally better at 37 degrees C and pH 7. Maximum removal was found at initial concentration of 0.5 to 2 mM, however, growth and Ni removal were affected by other heavy metals. Major amount of the metal was accumulated in the membrane fractions and certain negatively charged groups were found responsible for Ni binding. KUNi5 could also produce 1-aminocyclopropane-1-carboxylate deaminase, indole-acetic acid and siderophore...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/27282002/usefulness-of-chromagar-candida-medium-biochemical-methods-api-id32c-and-vitek-2-compact-and-two-maldi-tof-ms-systems-for-candida-spp-identification
#18
Elzbieta Stefaniuk, Anna Baraniak, Monika Fortuna, Waleria Hryniewicz
This study was conducted to compare of the yeasts identification results obtained with two new systems using the MALDI-TOF MS technique with the ones obtained using the routine identification methods of Candida spp. in clinical microbiology laboratories. All 124 Candida spp. isolates were recovered from the routine examination of clinical specimens in microbiological laboratories and collected in the Centre of Quality Control in Microbiology in Warsaw (Poland). Our findings confirm the high agreement (98%) of fungal identification using the standard, biochemistry laboratory methods and mass spectrometry technique...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/27282001/antimicrobial-activity-of-penicillin-g-and-n-acetylcystein-on-planktonic-and-sessile-cells-of-streptococcus-suis
#19
Ivette Espinosa, Michel Báez, Evelyn Lobo, Siomara Martínez, Marcelo Gottschalk
The aim of this study was to investigate the capacity of Streptococcus suis strains to form biofilms and to evaluate the antimicrobial activity of Penicillin G and N-acetylcystein (NAC) on both S. suis sessile and planktonic forms. Only non-typeable isolates of S. suis were correlated with a greater biofilm formation capacity. The MCI of Penicillin G and NAC required for inhibiting biofilm growth were higher than the required concentration for inhibiting planktonic growth. The combinations of NAC and Penicillin G showed a strong synergistic activity that inhibited biofilm formation and disrupted the pre-formed biofilm of S...
2016: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/27282000/assessment-of-the-microbiological-status-of-probiotic-products
#20
Anna Zawistowska-Rojek, Tomasz Zareba, Agnieszka Mrówka, Stefan Tyski
The aim of this study was to perform the microbiological analysis of quality of 25 probiotic products, available on the Polish market. Analysis of bacterial viability in probiotic products showed that not all of these preparations possess a suitable number of bacteria. Moreover, some of the tested probiotic products contained bacterial strains other than those declared by the manufacturer. All tested strains recovered from probiotic products were found to be resistant to metronidazole and susceptible to nitrofurantoin...
2016: Polish Journal of Microbiology
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