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Polish Journal of Microbiology

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https://www.readbyqxmd.com/read/28735321/betaine-improves-polymer-grade-d-lactic-acid-production-by-sporolactobacillus-inulinus-using-ammonia-as-green-neutralizer
#1
Guoping Lv, Chengchuan Che, Li Li, Shujing Xu, Wanyi Guan, Baohua Zhao, Jiansong Ju
The traditional CaCO3-based fermentation process generates huge amount of insoluble CaSO4 waste. To solve this problem, we have developed an efficient and green D-lactic acid fermentation process by using ammonia as neutralizer. The 106.7 g/L of D-lactic acid production and 0.89 g per g of consumed sugar were obtained by Sporolactobacillus inulinus CASD with a high optical purity of 99.7% by adding 100 mg/L betaine in the simple batch fermentation process. The addition of betaine was experimentally proven to protect cell at high concentration of ammonium ion, increase the D-lactate dehydrogenase specific activity and thus promote the production of D-lactic acid...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735320/in-vitro-interaction-between-fumonisin-b-sub-1-sub-and-the-intestinal-microflora-of-pigs
#2
Huu Anh Dang, Attila Zsolnai, Melinda Kovacs, Istvan Bors, Andras Bonai, Brigitta Bota, Judit Szabo Fodor
The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in the amount of bacterial groups between the experimental (buffer, chyme, and fumonisin B1) and control 1 groups (buffer + chyme) were observed in both methods...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735319/intracellular-siderophore-detection-in-an-egyptian-cobalt-treated-f-solani-isolate-using-sem-edx-with-reference-to-its-tolerance
#3
Rasha M Farrag
An Egyptian, plant pathogenic Fusarium solani isolate was grown on cobalt concentrations of 0, 50, 200, 500, 800, and 1000 ppm. The isolate survived concentrations up to 800 ppm, however failed to grow at 1000 ppm. Morphology and elemental analysis of the isolate under the investigated Co concentrations were studied using Scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). The isolate reserved its morphology up to a concentration of 200 ppm. Morphological distortions were dramatic at 500 and 800 ppm...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735318/trends-of-bloodstream-infections-in-a-university-greek-hospital-during-a-three-year-period-incidence-of-multidrug-resistant-bacteria-and-seasonality-in-gram-negative-predominance
#4
Fevronia Kolonitsiou, Matthaios Papadimitriou-Olivgeris, Anastasia Spiliopoulou, Vasiliki Stamouli, Vasileios Papakostas, Eleni Apostolopoulou, Christos Panagiotopoulos, Markos Marangos, Evangelos D Anastassiou, Myrto Christofidou, Iris Spiliopoulou
The aim of the study was to assess the epidemiology, the incidence of multidrug-resistant bacteria and bloodstream infections' (BSIs) seasonality in a university hospital. This retrospective study was carried out in the University General Hospital of Patras, Greece, during 2011-13 y. Blood cultures from patients with clinical presentation suggestive of bloodstream infection were performed by the BacT/ALERT System. Isolates were identified by Vitek 2 Advanced Expert System. Antibiotic susceptibility testing was performed by the disk diffusion method and E-test...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735317/genetic-characterization-of-a-novel-composite-transposon-carrying-arma-and-aac-6-ib-genes-in-an-escherichia-coli-isolate-from-egypt
#5
Mona T Kashef, Omneya M Helmy
Aminoglycosides are used in treating a wide range of infections caused by Gram-positive and Gram-negative bacteria; however, aminoglycoside resistance is common and occurs by several mechanisms. Among these mechanisms is bacterial rRNA methylation by the 16S rRNA methyl transferase (16S-RMTase) enzymes; but data about the spread of this mechanism in Egypt are scarce. Cephalosporins are the most commonly used antimicrobial agents in Egypt; therefore, this study was conducted to determine the frequency of 16S-RMTase among third generation cephalosporin-resistant clinical isolates in Egypt...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735316/biochar-rhizosphere-interactions-a-review
#6
Sławomir Głuszek, Lidia Sas Paszt, Beata Sumorok, Ryszard Kozera
Biochar is a solid material of biological origin obtained from the biomass carbonization, designed as a mean to reduce greenhouse gases emission and carbon sequestration in soils for a long time. Biochar has a wide spectrum of practical utilization and is applied as a promising soil improver or fertilizer in agriculture, or as a medium for soil or water remediation. Preparations of biochar increase plant growth and yielding when applied into soil and also improve plant growth conditions, mainly bio, physical and chemical properties of soil...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735315/the-diversity-growth-promoting-abilities-and-anti-microbial-activities-of-bacteria-isolated-from-the-fruiting-body-of-agaricus-bisporus
#7
Quanju Xiang, Lihua Luo, Yuhuan Liang, Qiang Chen, Xiaoping Zhang, Yunfu Gu
Agaricus bisporus plays an important role in ecological processes and is one of the most widely cultivated mushrooms worldwide. Mushroom growth-promoting bacteria have been isolated from casing soil and compost, but microorganisms in the fruiting body have received only a little attention. To get an overview of phylogenetic diversity of microorganisms in the fruiting body of A. bisporus, as well as to screen antimicrobial and mushroom growth-promoting strains, and eventually intensify mushroom production, we isolated and characterized microorganisms from the fruiting body of A...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735314/gene-expression-during-btex-biodegradation-by-a-microbial-consortium-acclimatized-to-unleaded-gasoline-and-a-pseudomonas-putida-strain-hm346961-isolated-from-it
#8
Jesus A Morlett-Chavez, Jose A Ascacio-Martinez, William E Haskins, Karim Acuña-Askar, Hugo A Barrera-Saldaña
Pseudomonas putida strain (HM346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. The consortium can efficiently remove benzene, toluene, ethylbenzene and xylene (BTEX) isomers, and a similar capability was observed with the P. putida strain. Proteome of this strain showed certain similarities with that of other strains exposed to the hydrocarbon compounds. Furthermore, the toluene di-oxygenase (tod) gene was up-regulated in P. putida strain when exposed to toluene, ethylbenzene, xylene, and BTEX...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735313/early-kpc-producing-klebsiella-pneumoniae-bacteremia-among-intensive-care-unit-patients-non-colonized-upon-admission
#9
Matthaios Papadimitriou-Olivgeris, Fotini Fligou, Iris Spiliopoulou, Christina Bartzavali, Vasiliki Dodou, Sophia Vamvakopoulou, Kyriaki Koutsileou, Anastasia Zotou, Evangelos D Anastassiou, Myrto Christofidou, Markos Marangos
Among 140 patients colonized by KPC-producing Klebsiella pneumoniae (KPC-Kp) between fourth and seventh day of Intensive Care Unit stay, 24 developed bacteraemia immediately after colonization. Colistin-resistance of the colonizing isolate was the factor significantly associated with early KPC-Kp bacteraemia (P < 0.001; OR 6.6, 95% CI 2.4-18.4), a worrisome finding since infections by colistin-resistant isolates is associated with increased mortality due to limited remaining therapeutic options.
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735312/effect-of-lyophilization-on-survivability-and-growth-kinetic-of-trichoderma-strains-preserved-on-various-agriculture-by-products
#10
Danuta Witkowska, Katarzyna Buska-Pisarek, Wojciech Łaba, Michał Piegza, Anna Kancelista
Growth of four Trichoderma strains were tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for their survival rate and growth after lyophilization on these carriers. All applied monocomponent and bicomponent media were substrates for the production and preservation of Trichoderma biomass. However, the maximum number of colony forming units (CFU/g dm) was acquired on bicomponent media based on dried grass and beet pulp or grass with corn cobs, when compared to monocomponent media...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735311/erythromycin-or-clindamycin-is-it-still-an-empirical-therapy-against-streptococcus-agalactiae-in-patients-allergic-to-penicillin
#11
Piotr Leszczyński, Beata Sokół-Leszczyńska, Bronisława Pietrzak, Anna Sawicka-Grzelak, Mirosław Wielgoś
Retrospective analysis of Streptococcus agalactiae antibiotic susceptibility isolated in 2010-2013 was performed. Penicillin was still the first-line antibiotic. Due to the high percentage of strains resistant to erythromycin and clindamycin empirical treatment with these antibiotics may not be effective. Lower resistance rate to erythromycin and clindamycin among strains isolated from infected pregnant women and newborns were observed than among strains isolated from samples from patients hospitalized in other departments (29% and 47% v...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735310/an-improve-protocol-for-pcr-using-lm1-and-lm2-primers-for-listeria-monocytogenes-detection-in-food-matrices
#12
Angélica Godínez-Oviedo, Gerardo M Nava, Sofía M Arvizu-Medrano, Montserrat Hernández-Iturriaga
Several studies have observed that a conventional PCR protocol using primers LM1 and LM2 for the identification of gene hlyA Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason in this study, we provide a modified PCR protocol that improves the specificity of the LM1 and LM2 primers.
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735309/safety-evaluation-of-enterocin-producer-enterococcus-sp-strains-isolated-from-traditional-turkish-cheeses
#13
Mine Avcı, Banu Özden Tuncer
The purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in Enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. Enterococcus strains were isolated from 33 different cheeses. Enterococcus faecium (6 strains) and Enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16S rDNA analyses. Structural genes entA, entB, entP and entX were detected in some isolates. Multiple enterocin structural genes were found in 7 strains...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735308/bactericidal-activity-of-octenidine-to-various-genospecies-of-borrelia-burgdorferi-sensu-lato-spirochetes-in-vitro-and-in-vivo
#14
Stanisława Tylewska-Wierzbanowska, Urszula Rogulska, Grażyna Lewandowska, Tomasz Chmielewski
The aim of our studies was to invent a reliable method for detection of the bactericidal activity of disinfectants against Borrelia burgdorferi in suspension (in vitro) and in cell line cultures (in vivo). In the suspension method, 0.01% octenidine at 20°C and 35°C was bactericidal to Borrelia afzeli; Borrelia garini, B. burgdorferi sensu stricto after 5 minutes treatment. Increase of the temperature to 35°C speed up the bactericidal effect to 1 minute. The bactericidal action of octenidine towards B. burgdorferi spirochetes growing in fibroblasts was less effective and needed a longer time to kill them than in the suspension...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735307/influence-of-pseudomonas-and-bacillus-strains-isolated-from-lolium-perenne-rhizospheric-soil-in-vojvodina-serbia-on-planth-growth-and-soil-microbial-communities
#15
Stamenov R Dragana, Simonida Đurić, Timea I Hajnal Jafari, Snezana Anđelković
The aim of this study was the isolation of Pseudomonas sp. and Bacillus sp. strains from rhizospheric soil and monitoring the impact of two isolates denoted as P12 (Pseudomonas sp.) and B1 (Bacillus sp.) on the parameters of English ryegrass (Lolium perenne) yield and activity of the soil microbial communities. During 2012-2014, a plot experiment was set up following the randomized block system. Better effect on the plant growth was recorded with the use of Pseudomonas sp. P12 isolate than with Bacillus sp...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735306/the-serological-surveillance-of-hepatitis-e-virus-among-hunters-and-foresters-in-eastern-poland
#16
Marcin Weiner, Małgorzata Tokarska-Rodak, Dorota Plewik, Anna Pańczuk, Adam Szepeluk, Monika Krajewska
The aim of the study was the analysis of the occurrence of specific antibodies against HEV among hunters and foresters who are at risk to be exposed. The study group consisted of 210 hunters (23-80 years old) and 60 foresters (22-64 years old). Anti-HEV IgG were present in 3.81% of the samples of the hunters and in 5% of the samples of the foresters. The statistical analysis shows no significant differences in the results anti-HEV IgG between the groups of hunters and foresters (p = 0.5278). Significantly higher anti-HEV IgG titers were found in the older age group (> 55 years old)...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735305/molecular-study-of-indigenous-bacterial-community-composition-on-exposure-to-soil-arsenic-concentration-gradient
#17
Semanti Basu, Tanima Paul, Priya Yadav, Abhijit Debnath, Keka Sarkar
Community structure of bacteria present in arsenic contaminated agricultural soil was studied with qPCR (quantitative PCR) and DGGE (Denaturing Gradient Gel Electrophoresis) as an indicator of extreme stresses. Copy number of six common bacterial taxa (Acidobacteria, Actinobacteria, α-, β- and γ-Proteobacteria, Firmicutes) was calculated using group specific primers of 16S rDNA. It revealed that soil contaminated with low concentration of arsenic was dominated by both Actinobacteria and Proteobacteria but a shift towards Proteobacteria was observed with increasing arsenic concentration, and number of Actinobacteria eventually decreases...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735304/morphological-and-molecular-characterization-of-phoma-complanata-a-new-causal-agent-of-archangelica-officinalis-hoffm-in-poland
#18
Beata Zimowska, Ewa D Zalewska, Ewa D Król, Agnieszka Furmańczyk
The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of P. complanata Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of the fungus to the species...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735334/detection-of-polioviruses-in-sewage-using-cell-culture-and-molecular-methods
#19
Agnieszka Figas, Magdalena Wieczorek, Bogumiła Litwińska, Włodzimierz Gut
The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally, we wanted to determine if sewage concentration affects the results obtained for RT-PCR and cell cultures...
January 2, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735333/survival-of-bacteria-in-respiratory-protective-filters
#20
Ewa B Miaśkiewicz-Pęska, Tomasz Słomczyński
The aim of this study was to estimate the susceptibility of Staphylococcus hominis bacteria to the bacteriostatic agent in respiratory protective filters. Four types of filter media of different characteristics were tested. The number of bacteria was estimated by a culture-based method. It was proved that in treated filters the number of S. hominis was significantly reduced, even below the detection limit, while in untreated material bacteria were able to grow and multiply up to 100-fold within 8 hours. There was no correlation between filter structure and changes in the number of bacterial cells...
January 2, 2017: Polish Journal of Microbiology
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