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Polish Journal of Microbiology

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https://www.readbyqxmd.com/read/29319529/seroprevalence-of-rubella-and-cytomegalia-in-young-women-from-bia%C3%A5-a-podlaska-district
#1
Dorota Plewik, Małgorzata Tokarska-Rodak, Justyna Paszkiewicz, Adam Szepeluk
The aim of this study was to analyze the seroprevalence of rubella and cytomegalia among young women. The study included 175 healthy women from the Biała Podlaska District, aged 16 to 35 years. Anti-rubella and anti-CMV IgG were determined by ELISA. 172 (98.3%) study subjects tested positive for rubella antibodies, 1 (0.6%) was seroindeterminate and 2 (1.1%) were seronegative. CMV antibodies were detected in 119 (68.0%) participants; the series included also 1 (0.6%) seroindeterminate and 55 (31.4%) seronegative women...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319528/metagenomic-profiling-of-the-bacterial-community-changes-from-koji-to-mash-stage-in-the-brewing-of-soy-sauce
#2
Hongbin Wang, Quanzeng Wei, Shuqi Gui, Yongrui Feng, Yong Zhang, Yihan Liu, Fuping Lu
The improvement of soy sauce fermentation is restricted by the insufficient information on bacterial community. In this study, bacterial communities in the koji and mash stage were compared based on next-generation sequencing technology. A total of 29 genera were identified in the koji stage, while 34 in the mash stage. After koji stage, 7 genera disappeared and 12 new genera appeared in the mash stage. The dominant bacteria were Kurthia, Weissella and Staphylococcus in the koji stage and Staphylococcus, Kurthia, Enterococcus and Leuconostoc in the mash stage...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319527/identification-of-lactobacillus-delbrueckii-and-streptococcus-thermophilus-strains-present-in-artisanal-raw-cow-milk-cheese-using-real-time-pcr-and-classic-plate-count-methods
#3
Milena A Stachelska
The aim of this paper was to detect Lactobacillus delbrueckii and Streptococcus thermophilus using real-time quantitative PCR assay in 7-day ripening cheese produced from unpasteurised milk. Real-time quantitative PCR assays were designed to identify and enumerate the chosen species of lactic acid bacteria (LAB) in ripened cheese. The results of molecular quantification and classic bacterial enumeration showed a high level of similarity proving that DNA extraction was carried out in a proper way and that genomic DNA solutions were free of PCR inhibitors...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319525/physiological-effects-of-whey-and-milk-based-probiotic-yogurt-in-rats
#4
Judit Szabó-Fodor, András Bónai, Brigitta Bóta, Linda Szommerné Egyed, Ferenc Lakatos, Gréta Pápai, Attila Zsolnai, Róbert Glávits, Katalin Horvatovich, Melinda Kovács
In an in vitro experiment commercially available probiotic products were tested for the survival of bacteria under conditions of simulated human digestion either when used alone or mixed into yogurt. In the in vivo experiment the effects of feeding a whey- and milk-based yogurt prepared with the probiotic strain showing adequate survival in the in vitro experiment, was measured on body weight, feed con¬sumption and immune response of rats (IgG and IgA level after immunisation), on the composition and volatile fatty acid production of the intestinal microbiota and on the structure of intestinal villi...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319523/temperature-ph-and-trimethoprim-sulfamethoxazole-are-potent-inhibitors-of-biofilm-formation-by-stenotrophomonas-maltophilia-clinical-isolates
#5
Marjan Biočanin, Haowa Madi, Zorica Vasiljević, Milan Kojić, Branko Jovčić, Jelena Lozo
Stenotrophomonas maltophilia, an opportunistic pathogen usually connected with healthcare-associated infections, is an environmental bacterium. Intrinsic resistance to multiple antibiotics, with different virulence determinants in the last decade classified this bacterium in the group of global multiple drug resistant (MDR) organism. S. maltophilia clinical isolates, were collected from tertiary care pediatric hospital in Belgrade, Serbia to investigate influence of different factors on biofilm formation, kinetics of biofilm formation for strong biofilm producers and effect of trimethoprim-sulfamethoxazole (TMP/SMX) on formed biofilm...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319522/characterization-of-microbial-communities-in-acidified-sulfur-containing-soils
#6
Grzegorz Czerwonka, Iwona Konieczna, Paulina Żarnowiec, Artur Zieliński, Agnieszka Malinowska-Gniewosz, Agnieszka Gałuszka, Zdzisław Migaszewski, Wiesław Kaca
Over a period of three years, microbial communities in acidified soil with high sulfur content were analyzed. In soil water extracts ureolytic, proteolytic, oxidoreductive, and lipolytic activity were detected. The presented results indicate that the enzymatic activity of soil microbial communities varied considerably over time. Isolated 26 (80%) bacterial strains belonged to genus Bacillus sp. and were identified by cultivation and 16S rRNA methods. The commercially available procedures for bacterial DNA isolation from acidified soil failed, therefore a new, specific DNA isolation method was established...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319519/analyses-of-plasmids-harbouring-quinolone-resistance-determinants-in-enterobacteriaceae-members
#7
Bayri Erac, Fethiye Ferda Yilmaz, Ismail Ozturk, Sabire Sohret Aydemir, Mine Hosgor-Limoncu
The aim of this study was to explore the plasmid characteristics of eight clinical Enterobacteriaceae strains containing extended broad spectrum beta-lactamases and plasmid-mediated quinolone resistance. Plasmids were transferred by conjugation or transformation and resistance determinants were investigated by PCR. We showed that at least one plasmid harbouring qnrB or qnrS determinant was transferred by conjugation in five isolates. QepA determinant was confirmed to be on a non-conjugative plasmid. We found at least one beta-lactamase gene in seven of the eight clinical isolates having plasmid-mediated quinolone resistance, which indicated that these two resistance determinants were mostly on the same conjugative plasmids...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319517/characterisation-of-antimicrobial-properties-of-extracts-of-selected-medicinal-plants
#8
Monika Cioch, Paweł Satora, Magdalena Skotniczny, Dorota Semik-Szczurak, Tomasz Tarko
The scope of the experiments included analysis of the antimicrobial activity of ethanolic, methanolic and aqueous extracts against bacterial and fungal cultures and determination of the minimum inhibitory concentration of plant extracts tested microbial growth. Analysis of the antifungal and antibacterial activity was carried out by the disc diffusion method using paper discs. In the experiment 11 species of microorganisms - 8 bacterial and 3 fungal strains were used. The highest antimicrobial activity against the tested strains was demonstrated by black elder (Sambucus nigra L...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319515/the-determination-and-correlation-of-various-virulence-genes-esbl-serum-bactericidal-effect-and-biofilm-formation-of-clinical-isolated-classical-klebsiella-pneumoniae-and-hypervirulent-klebsiella-pneumoniae-from-respiratory-tract-infected-patients
#9
Rambha K Shah, Zhao H Ni, Xiao Y Sun, Guo Q Wang, Fan Li
Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent K. pneumoniae (hvKP) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and biofilm formation of clinical isolated cKP and hvKP from respiratory tract infected patients. A total of 96 K. pneumoniae strains were isolated from sputum of respiratory tract infected patients...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319514/characterization-of-bacteriocin-producing-lactic-acid-bacteria-isolated-from-native-fruits-of-ecuadorian-amazon
#10
Karina Garzón, Clara Ortega, Gabriela N Tenea
Tropical, wild-type fruits are considered biodiverse "hotspots" of microorganisms with possible functional characteristics to be investigated. In this study, several native lactic acid bacteria (LAB) of Ecuadorian Amazon showing highly inhibitory potential were identified and characterized. Based on carbohydrate fermentation profile and 16S rRNA gene sequencing, seven strains were assigned as Lactobacillus plantarum and one strain as Weissella confusa. Using agar-well diffusion method the active synthetized components released in the neutralized and hydroxide peroxide eliminated cell-free supernatant were inhibited by proteolytic enzymes, while the activity was maintained stable after the treatment with catalase, lysozyme, α-amylase and lipase suggesting their proteinaceous nature...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319513/production-characterization-and-valuable-applications-of-exopolysaccharides-from-marine-bacillus-subtilis-sh1
#11
Sahar W M Hassan, Hassan A H Ibrahim
Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic. Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields. The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319511/study-of-patterns-and-markers-of-human-immune-deficiency-virus-1-hiv-1-progression-and-unemployment-rate-among-patients-from-alexandria-egypt
#12
Faika M Ghoneim, May M Raouf, Noha S Elshaer, Sarah M Abdelhamid, Reem A Noor Eldeen
Middle East and North Africa (MENA) new HIV cases show the highest increase among all regions in the world. Even though Egypt has a low prevalence among the general population (< 0.02%), a national HIV epidemic occurs in certain population risk groups. The current study was conducted to asses clinical and immunological disease progression; following up viral load (VL) and detecting delta-32 CCR5 genotype polymorphism in selected cases, determining unemployment rate and identify predictors of employment for HIV-cases...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319509/prevalence-influencing-factors-antibiotic-resistance-toxin-and-molecular-characteristics-of-staphylococcus-aureus-and-mrsa-nasal-carriage-among-diabetic-population-in-the-united-states-2001-2004
#13
Jialing Lin, Yang Peng, Chan Bai, Ting Zhang, Haoqu Zheng, Xiaojie Wang, Jiaping Ye, Xiaohua Ye, Ying Li, Zhenjiang Yao
Diabetic population were reported more likely to suffer carriage and infection with Staphylococcus aureus (S. aureus) and methicillin-resistant Staphylococcus aureus (MRSA) than non-diabetic population. We aim to elucidate the prevalence and characteristics of S. aureus and MRSA nasal carriage among diabetic population in the United States National Health and Nutrition Examination Survey, 2001-2004. Univariate analyses were conducted using Chi-square test, Fisher's exact probability test or student t test, as appropriate...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319507/inhibition-of-multidrug-resistant-gram-positive-and-gram-negative-bacteria-by-a-photoactivated-porphyrin
#14
Moreno Bondi, Anna Mazzini, Simona de Niederhäusern, Ramona Iseppi, Patrizia Messi
The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecyl-pyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable susceptibility of S. aureus and E. faecalis to treatment while, under the same conditions, E. coli and P. aeruginosa showed very low susceptibility...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29313518/transferrin-and-lactoferrin-human-iron-sources-for-enterococci
#15
Paweł Lisiecki
To overcome limitations in iron acquisition, enterococci have evolved a number of mechanisms to scavenge iron from the host iron-binding proteins - transferrin (TR) and lactoferrin (LF). The aim of this study was to demonstrate the mechanisms by which enterococci utilize human TR and LF bound iron. The study included two strains of Enterococcus faecalis grown in iron-deficient and iron-excess media respectively. The binding activity of both proteins was monitored using proteins labelled with 125I. The uptake of iron by enterococci was determined using 59Fe labelled proteins...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29313517/the-prevalence-of-exoenzyme-s-gene-in-multidrug-sensitive-and-multidrug-resistant-pseudomonas-aeruginosa-clinical-strains
#16
Tomasz Bogiel, Aleksander Deptuła, Joanna Kwiecińska-Piróg, Małgorzata Prażyńska, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska
Pseudomonas aeruginosa rods are one of the most commonly isolated microorganisms from clinical specimens, usually responsible for nosocomial infections. Antibiotic-resistant P. aeruginosa strains may present reduced expression of virulence factors. This fact may be caused by appropriate genome management to adapt to changing conditions of the hospital environment. Virulence factors genes may be replaced by those crucial to survive, like antimicrobial resistance genes. The aim of this study was to evaluate, using PCR, the occurrence of exoenzyme S-coding gene (exoS) in two distinct groups of P...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319532/the-ability-of-a-novel-strain-scheffersomyces-syn-candida-shehatae-isolated-from-rotten-wood-to-produce-arabitol
#17
Monika Kordowska-Wiater, Adam Kuzdraliński, Tomasz Czernecki, Zdzisław Targoński, Magdalena Frąc, Karolina Oszust
Arabitol is a polyalcohol which has about 70% of the sweetness of sucrose and an energy density of 0.2 kcal/g. Similarly to xylitol, it can be used in the food and pharmaceutical industries as a natural sweetener, a texturing agent, a dental caries reducer, and a humectant. Biotechnological production of arabitol from sugars represents an interesting alternative to chemical production. The yeast Scheffersomyces shehatae strain 20BM-3 isolated from rotten wood was screened for its ability to produce arabitol from L-arabinose, glucose, and xylose...
September 27, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319531/metagenomic-analysis-of-soil-bacterial-community-and-level-of-genes-responsible-for-biodegradation-of-aromatic-hydrocarbons
#18
Jakub Czarny, Justyna Staninska-Pięta, Jolanta Powierska-Czarny, Jacek Nowak, Łukasz Wolko, Agnieszka Piotrowska-Cyplik
The aim of the studies was to compare the composition of soil bacterial metabiomes originating from urbanized areas and areas con¬taminated with hydrocarbons with those from agricultural soil and forest soil obtained from a protected wild-life park area. It should be noted that hydrocarbons are everywhere therefore bacteria capable of their utilization are present in every soil type. In the hydrocarbon-contaminated soil and in the soil of anthropogenic origin, the bacteria belonging to Gammaproteobacteria were dominant (28...
September 27, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319530/coomassie-blue-g250-for-visualization-of-active-bacteria-from-lake-environment-and-culture
#19
Bartosz Kiersztyn, Waldemar Siuda, Ryszard Chróst
Bacteria play a fundamental role in the cycling of nutrients in aquatic environments. A precise distinction between active and inactive bacteria is crucial for the description of this process. We have evaluated the usefulness of Coomassie Blue G250 for fluorescent staining of protein containing potentially highly active bacteria. We found that the G250 solution has excitation and emission properties appropriate for direct epifluorescence microscopy observations. It enables fast and effective fluorescent visualization of living, protein-rich bacteria, both in freshwater environment and culture...
September 27, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319526/mrsa-in-pig-population
#20
Meldra Ivbule, Edvīns Miklaševičs, Liene Čupāne, Laima Bērziņa, Andris Bāliņš, Anda Valdovska
Methicillin resistant Staphylococcus aureus (MRSA) is widespread worldwide in different types of animal species and as a zoonosis takes a great risk for human health not only as a food toxicoinfection, but also as a highly resistant pathogen causing serious soft tissue infectious, septicaemia and even death. One of the most affected food-producing animal species is swine in the production of which new antibiotics in big amounts are used more and more continuously, increasing antimicrobial resistance. In this study several commercial pig farms and pigs with different age groups as well as farm workers and samples from environment were examined with the purpose of detecting MRSA prevalence and evaluating antimicrobial resistance...
September 27, 2017: Polish Journal of Microbiology
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