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Zebrafish

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https://www.readbyqxmd.com/read/28422577/enrichment-of-zebrafish-danio-rerio-hamilton-1822-diet-with-polyunsaturated-fatty-acids-improves-fecundity-and-larvae-quality
#1
Joanna Nowosad, Dariusz Kucharczyk, Katarzyna Targońska
The zebrafish is a freshwater fish of the Cyprinidae family, which is frequently used in scientific research. It owes its popularity to its genome, whose structure is comparable to the human genome and, for this reason, this species is often used in human medical research. However, such research requires high-quality material to conduct tests producing repeatable results. This study examines the effect of providing feed enriched with essential fatty acids, especially docosahexaenoic acid, to zebrafish spawners on fertility as well as the survival rate and growth of their offspring...
April 19, 2017: Zebrafish
https://www.readbyqxmd.com/read/28394248/highest-diploid-number-among-gymnotiformes-first-cytogenetic-insights-into-rhabdolichops-sternopygidae
#2
Pablo Suárez, Inaê Cristina Guerreiro Pinto Barroso, Danillo Dos Santos Silva, Susana S R Milhomem, Diogo Cavalcanti Cabral-de-Mello, Cesar Martins, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi
We report the first comparative cytogenetic analysis of two species from electrogenic fish of genus Rhabdolichops (Sternopygidae, Gymnotiformes): Rhabdolichops troscheli and Rhabdolichops cf eastwardi. R. troscheli has 2n = 54 (fundamental number [FN] = 66), whereas R. cf. eastwardi has 2n = 74 (FN = 78). C-banding revealed centromeric constitutive heterochromatin in both species. Ag-NORs mapped on pair 6 in R. troscheli and pair 30 in R. cf eastwardi. Fluorescense in situ hybridization with 18S rDNA probes confirmed the Ag-NOR staining results and revealed additional (presumably silent) ribosomal genes on pairs 12, 13, 21, 23, 26, and 27 in R...
April 10, 2017: Zebrafish
https://www.readbyqxmd.com/read/28394244/a-zebrafish-model-of-human-fibrodysplasia-ossificans-progressiva
#3
Melissa LaBonty, Nicholas Pray, Pamela C Yelick
Fibrodysplasia ossificans progressiva (FOP) is a rare, autosomal dominant genetic disorder in humans characterized by explosive inflammatory response to injury leading to gradual ossification within fibrous tissues, including skeletal muscle, tendons, and ligaments. A variety of animal models are needed to study and understand the etiology of human FOP. To address this need, here we present characterizations of the first adult zebrafish model for FOP. In humans, activating mutations in the Type I BMP/TGFβ family member receptor, ACVR1, are associated with FOP...
April 10, 2017: Zebrafish
https://www.readbyqxmd.com/read/28355106/chromosomal-mapping-of-repetitive-dna-sequences-in-the-genus-bryconamericus-characidae-and-dna-barcoding-to-differentiate-populations
#4
Angélica Rossotti Dos Santos, Mariana Campaner Usso, Juceli Gonzalez Gouveia, Cristian Araya-Jaime, Wilson Frantine-Silva, Lucia Giuliano-Caetano, Fausto Foresti, Ana Lúcia Dias
The mapping of repetitive DNA sites by fluorescence in situ hybridization has been widely used for karyotype studies in different species of fish, especially when dealing with related species or even genera presenting high chromosome variability. This study analyzed three populations of Bryconamericus, with diploid number preserved, but with different karyotype formulae. Bryconamericus ecai, from the Forquetinha river/RS, presented three new cytotypes, increasing the number of karyotype forms to seven in this population...
March 29, 2017: Zebrafish
https://www.readbyqxmd.com/read/28350247/the-transgenic-zebrafish-display-fluorescence-reflecting-the-expressional-dynamics-of-dihydrofolate-reductase
#5
Wen-Ni Chang, Wan-Yu Chi, Tseng-Ting Kao, Jen-Ning Tsai, Wangta Liu, Shih-Shin Liang, Chien-Chih Chiu, Bing-Hung Chen, Tzu-Fun Fu
Dihydrofolate reductase (DHFR) reduces folic acid and recycles dihydrofolate generated during dTMP biosynthesis to tetrahydrofolate. DHFR is upregulated in rapidly proliferating cells and hence a favored target of antifolate drug against cancers, autoimmune diseases, and microbial infections. However, increased expression of dhfr contributed to the often emerging drug resistance and impeded the therapeutic efficacy of antifolate drugs. Therefore, comprehensive knowledge on the expressional control of dhfr becomes crucial...
March 28, 2017: Zebrafish
https://www.readbyqxmd.com/read/28318435/determining-zebrafish-epitope-reactivity-to-commercially-available-antibodies
#6
Michael A Villarreal, Nicole M Biediger, Natalie A Bonner, Jennifer N Miller, Samantha K Zepeda, Benjamin J Ricard, Dana M García, Karen A Lewis
Antibodies raised against mammalian proteins may exhibit cross-reactivity with zebrafish proteins, making these antibodies useful for fish studies. However, zebrafish may express multiple paralogues of similar sequence and size, making them difficult to distinguish by traditional Western blot analysis. To identify the zebrafish proteins that are recognized by an antimammalian antibody, we developed a system to screen putative epitopes by cloning the sequences between the yeast SUMO protein and a C-terminal 6xHis tag...
March 20, 2017: Zebrafish
https://www.readbyqxmd.com/read/28318417/itfish-enhanced-staining-by-iterative-fluorescent-in-situ-hybridization
#7
Richard H Row, Benjamin L Martin
Fluorescent in situ hybridization (FISH) is an important tool for zebrafish research, particularly when observing the expression of two different genes in the same embryo. Peroxidase-catalyzed deposition of tyramide-conjugated dyes is a widely used and cost-effective approach to performing FISH. A major limitation of the technique is that it does not work well for weakly expressed genes. Here we present a method adapted from planarian research for use in zebrafish that provides a dramatic enhancement of weak staining...
March 20, 2017: Zebrafish
https://www.readbyqxmd.com/read/28318412/effects-of-lithium-and-2-4-dichlorophenol-on-zebrafish-circadian-rhythm-disorder-and-molecular-effects
#8
Bo Xiao, Li-Qiang Cui, Cheng Ding, Han Wang
The aim of this study was to investigate lithium and 2,4-dichlorophenol (2,4-DCP)-induced circadian rhythm disorder and their genome-wide effects in zebrafish. Zebrafish larvae were exposed to 250 ppm LiCl (n = 40) or 20 ppm 2,4-DCP. RNA was subsequently extracted and determined quantitatively. The mRNA levels of circadian clock-related genes, including clock1a, bmal1b, per2, and per1b, were determined. Microarray datasets were generated and the differentially expressed genes (DEGs) were identified. The mRNA levels of some upregulated and downregulated DEGs were examined by quantitative real-time polymerase chain reaction (RT-PCR)...
March 20, 2017: Zebrafish
https://www.readbyqxmd.com/read/28296621/optical-projection-tomography-as-a-novel-method-to-visualize-and-quantitate-whole-brain-patterns-of-cell-proliferation-in-the-adult-zebrafish-brain
#9
Benjamin W Lindsey, Jan Kaslin
How distinct cell populations are distributed in three-dimensional space under homeostasis or following injury, neurodegeneration, or with senescence can teach us much about brain-wide patterns and signaling along the neuroaxis. Visualizing individual cell populations in the mature vertebrate central nervous system (CNS) has remained a challenge as a result of difficulty clearing adult brain tissue or limitations in imaging depth or resolution. We have developed a simple clearing and imaging pipeline optimally suited for the adult zebrafish brain to investigate changes in patterns of cell proliferation in wild-type and transgenic backgrounds that can easily be quantified and represented using FIJI and IMARIS software...
March 15, 2017: Zebrafish
https://www.readbyqxmd.com/read/28287927/three-dimensional-printed-fish-graders-a-tool-to-rapidly-and-reliably-size-select-zebrafish
#10
Thomas O'Reilly-Pol, Kyle Kniepkamp, Stephen L Johnson
Research into adult zebrafish often requires fish to be of a specific size. Currently, fish must be individually measured to achieve this goal. Here, we design and utilize fish graders to quickly sort fish by width. We characterize graders individually for the length of fish they discriminate between and we also analyze graders in pairs to define the range of lengths for a retained population of fish. We note that a 1 mm increase of fish width increases fish length by 6.2-7.2 mm. We provide the schematics to print a series of eight retention widths, and note that graders of any desired retention width can easily be printed by slightly modifying our design files...
March 13, 2017: Zebrafish
https://www.readbyqxmd.com/read/28277948/karyotypic-evolution-and-chromosomal-organization-of-repetitive-dna-sequences-in-species-of-panaque-panaqolus-and-scobinancistrus-siluriformes-and-loricariidae-from-the-amazon-basin
#11
Thayana Ayres-Alves, Adauto Lima Cardoso, Cleusa Yoshiko Nagamachi, Leandro Melo de Sousa, Julio Cesar Pieczarka, Renata Coelho Rodrigues Noronha
Loricariidae family comprises the greatest variability of Neotropical catfish species, with more than 800 valid species. This family shows significant chromosomal diversity. Mapping of repetitive DNA sequences can be very useful in exploring such diversity, especially among groups that appear to share a preserved karyotypic macrostructure. We describe the karyotypes of Panaque armbrusteri and Panaqolus sp., as assessed using classical cytogenetic methods. Moreover, we offer a map of their repetitive sequences, including 18S and 5S ribosomal DNAs, the Rex1 and Rex3 retrotransposons, and the Tc1-mariner transposon in P...
March 9, 2017: Zebrafish
https://www.readbyqxmd.com/read/28266909/exposure-to-1-2-propanediol-impacts-early-development-of-zebrafish-danio-rerio-and-induces-hyperactivity
#12
Andrey Massarsky, Ayham Abdel, Lilah Glazer, Edward D Levin, Richard T Di Giulio
The use of electronic cigarettes (e-cigarettes) is increasing as an alternative to tobacco burning cigarettes; however, their safety remains to be fully determined. The long-term effects of e-cigarettes are unknown, including the effects of maternal e-cigarette use on pre- and postnatal development. Additional research on the safety of e-cigarettes is needed. Especially useful would be information from high- and moderate-throughput economic model systems. This study investigates the effects of 1,2-propanediol, which was identified as the main component of e-cigarette liquid, on early development of zebrafish (an in vivo high-throughput model system that was recently proposed for the study of tobacco cigarette and e-cigarette toxicity)...
March 7, 2017: Zebrafish
https://www.readbyqxmd.com/read/28192065/vascular-endothelial-growth-factor-a-and-leptin-expression-associated-with-ectopic-proliferation-and-retinal-dysplasia-in-zebrafish-optic-pathway-tumors
#13
Laura E Schultz, Staci L Solin, Wesley A Wierson, Janna M Lovan, Judith Syrkin-Nikolau, Deborah E Lincow, Andrew J Severin, Donald S Sakaguchi, Maura McGrail
In the central nervous system injury induces cellular reprogramming and progenitor proliferation, but the molecular mechanisms that limit regeneration and prevent tumorigenesis are not completely understood. We previously described a zebrafish optic pathway tumor model in which transgenic Tg(flk1:RFP)is18/+ adults develop nonmalignant retinal tumors. Key pathways driving injury-induced glial reprogramming and regeneration contributed to tumor formation. In this study, we examine a time course of proliferation and present new analyses of the Tg(flk1:RFP)is18/+ dysplastic retina and tumor transcriptomes...
February 13, 2017: Zebrafish
https://www.readbyqxmd.com/read/28192063/karyotype-diversity-in-doradidae-siluriformes-doradoidea-and-presence-of-the-heteromorphic-zz-zw-sex-chromosome-system-in-the-family
#14
Fábio Hiroshi Takagui, Lucas Ferreira de Moura, Daniela Cristina Ferreira, Liano Centofante, Carla de Andrade Vitorino, Vanessa Bueno, Vladimir Pavan Margarido, Paulo Cesar Venere
Karyotypes and other chromosomal markers as revealed by conventional and molecular cytogenetic protocols in four species of the catfish family Doradidae from the Araguaia-Tocantins river basin, namely Hassar wilderi, Leptodoras cataniae, Tenellus leporhinus and Tenellus trimaculatus were examined. All species had diploid chromosome number 2n = 58 and karyotypes dominated by biarmed chromosomes, simple NOR phenotype, that is, one chromosome pair bearing this site in terminal position, but some differences in karyotypes and distribution of constitutive heterochromatin, position of rDNA sites...
February 13, 2017: Zebrafish
https://www.readbyqxmd.com/read/28192066/performance-of-debaryomyces-hansenii-as-a-diet-for-rotifers-for-feeding-zebrafish-larvae
#15
Rafael Opazo, Karen Fuenzalida, Francisca Plaza-Parrochia, Jaime Romero
The zebrafish larval stage is a critical moment due to high mortality rates associated with inadequate supplies of nutritional requirements. Larval feeding has important challenges associated with such factors as small mouth gape (≈100 μm), the low activity of digestive enzymes, and the intake of live food. A common zebrafish live food at the onset of exogenous feeding is rotifers, mainly Brachionus plicatilis. These rotifers should be fed with other microorganisms such as microalgae or yeast, mostly from the Saccharomyces genus...
April 2017: Zebrafish
https://www.readbyqxmd.com/read/28186458/correction-to-zebrafish-2016-13-545-547
#16
(no author information available yet)
No abstract text is available yet for this article.
April 2017: Zebrafish
https://www.readbyqxmd.com/read/28151699/cytogenetic-and-molecular-data-support-the-occurrence-of-three-gymnotus-species-gymnotiformes-gymnotidae-used-as-live-bait-in-corumb%C3%A3-brazil-implications-for-conservation-and-management-of-professional-fishing
#17
Tatiane Pires de Sousa, Débora Karla Silvestre Marques, Carla de Andrade Vitorino, Karina de Cassia Faria, Gisele da Silva Ferreira Braga, Daniela Cristina Ferreira, Paulo Cesar Venere
In the Pantanal of Mato Grosso do Sul, electric fish (Gymnotus spp.) are the primary source of live bait, accounting for more than three-quarters of total sales. Based on chromosomal and molecular markers, the present study attempted to identify the Gymnotus species used as bait in the region of Corumbá, Mato Grosso do Sul, Brazil. Three species were detected, based on their distinct karyotypes: G. paraguensis (2n = 54), G. sylvius (2n = 40), and G. pantanal (2n = 39-40, X1X2Y/X1X1X2X2), with no evidence being found of interspecific hybrids...
April 2017: Zebrafish
https://www.readbyqxmd.com/read/28151697/microstructured-surface-arrays-for-injection-of-zebrafish-larvae
#18
Felix Ellett, Daniel Irimia
Microinjection of zebrafish larvae is an essential technique for delivery of treatments, dyes, microbes, and xenotransplantation into various tissues. Although a number of casts are available to orient embryos at the single-cell stage, no device has been specifically designed to position hatching-stage larvae for microinjection of different tissues. In this study, we present a reusable silicone device consisting of arrayed microstructures, designed to immobilize 2 days postfertilization larvae in lateral, ventral, and dorsal orientations, while providing maximal access to target sites for microinjection...
April 2017: Zebrafish
https://www.readbyqxmd.com/read/28135163/optimal-anesthetic-regime-for-motionless-three-dimensional-image-acquisition-during-longitudinal-studies-of-adult-nonpigmented-zebrafish
#19
Nicola Lockwood, Jennifer Parker, Carole Wilson, Paul Frankel
With many live imaging techniques, it is crucial that a deep level of anesthesia is reached and maintained throughout image acquisition without reducing zebrafish viability. This is particularly true for three-dimensional tomographic imaging modalities. Currently, the most commonly used anesthetic in the zebrafish community, MS-222 (tricaine methanesulfonate), does not allow this. We show, using a combination of both MS-222 and isoflurane, that we can significantly improve the anesthetic regime required for motionless image acquisition of live adult zebrafish...
April 2017: Zebrafish
https://www.readbyqxmd.com/read/28092494/zebrafish-are-able-to-detect-ethanol-in-their-environment
#20
Steven Tran, Hayden Chow, Benjamin Tsang, Amanda Facciol, Prabhlene Gandhi, Priyanka Desai, Robert Gerlai
Zebrafish have become a popular animal model for studying the development of alcohol addiction. Several behavioral paradigms for studying alcohol addiction have been developed for zebrafish, including conditioned place preference, alcohol-induced tolerance, and withdrawal. However, alcohol choice preference tasks have not been established in zebrafish as of yet. The ability of zebrafish to detect alcohol in their environment is required in alcohol choice or preference tasks. To our knowledge, it is currently unknown whether zebrafish are able to detect alcohol in their environment immediately following bath immersion...
April 2017: Zebrafish
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