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Rafael Antonio Vargas
Heart rate (HR) is a periodic activity that is variable over time due to intrinsic cardiac factors and extrinsic neural control, largely by the autonomic nervous system. Heart rate variability (HRV) is analyzed by measuring consecutive beat-to-beat intervals. This variability can contain information about the factors regulating cardiac activity under normal and pathological conditions, but the information obtained from such analyses is not yet fully understood. In this article, HRV in zebrafish larvae was evaluated under normal conditions and under the effect of substances that modify intrinsic cardiac activity and cardiac activity modulated by the nervous system...
December 5, 2016: Zebrafish
Fernanda Moreira Lopes, Sergiane Souza Caldas, Ednei Gilberto Primel, Carlos Eduardo da Rosa
It has been demonstrated that glyphosate-based herbicides are toxic to animals. In the present study, reactive oxygen species (ROS) generation, antioxidant capacity against peroxyl radicals (ACAP), and lipid peroxidation (LPO), as well as the activity and expression of the acetylcholinesterase (AChE) enzyme, were evaluated in Danio rerio males exposed to 5 or 10 mg/L of glyphosate for 24 and 96 h. An increase in ACAP in gills after 24 h was observed in the animals exposed to 5 mg/L of glyphosate. A decrease in LPO was observed in brain tissue of animals exposed to 10 mg/L after 24 h, while an increase was observed in muscle after 96 h...
December 1, 2016: Zebrafish
Seyed-Mohammadreza Samaee, Sahar Seyedin, Zoltan M Varga
Intraperitoneal (IP) injections are an effective and reproducible route of drug administration. However, current IP equipment can be either costly, inaccurate, or unsafe for zebrafish. We describe a simple, low-cost IP setup, which can be easily assembled from inexpensive and readily available parts, and which provides a safe, reproducible, and accurate IP-injection method for experimenters.
November 14, 2016: Zebrafish
Stephanie Grainger, Brianna Lonquich, Chet Huan Oon, Nicole Nguyen, Karl Willert, David Traver
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has been applied to edit genomes in a wide variety of model systems. Although this process can be quite efficient, editing at precise locations in the genome remains difficult without a suitable single guide RNA (sgRNA). We have developed a method for screening sgRNA function in vitro, using reagents that most zebrafish laboratories are already using. The results from our in vitro assay correlate with function in vivo in every sgRNA that we have examined so far...
November 9, 2016: Zebrafish
Fei Tieng Lim, Satoshi Ogawa, A Ian Smith, Ishwar S Parhar
The central nervous system (CNS) of the non-mammalian vertebrates has better neuroregenerative capability as compared with the mammalian CNS. Regeneration of habenula was observed 40 days after damage in zebrafish. During the early stage of regeneration, we found a significant increase of apoptotic cells on day-1 post-damage and of proliferative cells on day-3 post-damage. To identify the molecular factor(s) involved in the early stages of neuroregeneration, differentially expressed proteins during sham, 20- and 40-h post-habenula damage were investigated by proteomic approach by using two-dimensional differential gel electrophoresis (2D-DIGE) coupled with Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight (MALDI-ToF) and tandem mass spectrometry...
October 31, 2016: Zebrafish
Carl Breacker, Iain Barber, William H J Norton, Jonathan R McDearmid, Ceinwen A Tilley
Fin clipping of live fish under anesthesia is widely used to collect samples for DNA extraction. An alternative, potentially less invasive, approach involves obtaining samples by swabbing the skin of nonanesthetized fish. However, this method has yet to be widely adopted for use in laboratory studies in the biological and biomedical sciences. Here, we compare DNA samples from zebrafish Danio rerio and three-spined sticklebacks Gasterosteus aculeatus collected via fin clipping and skin swabbing techniques, and test a range of DNA extraction methods, including commercially available kits and a lower-cost, in-house method...
October 27, 2016: Zebrafish
Matthew D Keefe, Joshua L Bonkowsky
There has been a rapid expansion in use of transgenic technologies in zebrafish. We report a novel example of trans-interactions of genetic elements, or transvection. This interaction led to a novel expression pattern and illustrates a precautionary example regarding use of transgenes in zebrafish.
October 25, 2016: Zebrafish
Kayla Huemer, Jayne M Squirrell, Robert Swader, Danny C LeBert, Anna Huttenlocher, Kevin W Eliceiri
Zebrafish, an established model organism in developmental biology, is also a valuable tool for imaging wound healing in space and time with cellular resolution. However, long-term imaging of wound healing poses technical challenges as wound healing occurs over multiple temporal scales. The traditional strategy of larval encapsulation in agarose successfully limits sample movement but impedes larval development and tissue regrowth and is therefore not amenable to long-term imaging of wound healing. To overcome this challenge, we engineered a functionally compartmentalized device, the zebrafish Wounding and Entrapment Device for Growth and Imaging (zWEDGI), to orient larvae for high-resolution microscopy, including confocal and second harmonic generation (SHG), while allowing unrestrained tail development and regrowth...
September 27, 2016: Zebrafish
Jamille A Bitencourt, Iracilda Sampaio, Robson T C Ramos, Marcelo Ricardo Vicari, Paulo Roberto A de M Affonso
American soles (family Achiridae) have been characterized by remarkable chromosomal variation even though several species lack basic cytogenetic information. This trend indicates that chromosomal traits can be useful to taxonomy once the morphological identification of some taxa in this family (e.g., Achirus species) is controversial. In this work, we expand the cytogenetic data in Achiridae by providing the first karyotypic analysis of Achirus achirus. An unusual multiple sex chromosome system (X1X1X2X2/X1X2Y) was detected, once males presented 2n = 33 with three unpaired chromosomes (X1X2Y) while females presented 2n = 34 with two acrocentric pairs (X1X1 and X2X2) found in hemizygosis in males...
September 27, 2016: Zebrafish
João Gabriel Santos da Rosa, Heloísa Helena de Alcântara Barcellos, Renan Idalencio, Alessandra Marqueze, Michele Fagundes, Mainara Rossini, Cristiane Variani, Francine Balbinoti, Tássia Michele Huff Tietböhl, Denis Broock Rosemberg, Leonardo José Gil Barcellos
In this study, we show that an adaptation of the spinning test can be used as a model to study the exercise-exhaustion-recovery paradigm in fish. This forced swimming test promotes a wide range of changes in the hypothalamus-pituitary-interrenal axis functioning, intermediary metabolism, as well in fish behavior at both exercise and recovery periods. Our results pointed that this adapted spinning test can be considered a valuable tool for evaluating drugs and contaminant effects on exercised fish. This can be a suitable protocol both to environmental-to evaluate contaminants that act in fish energy mobilization and recovery after stressors-and translational perspectives-effects of drugs on exercised or stressed humans...
September 27, 2016: Zebrafish
Emily K Don, Isabel Formella, Andrew P Badrock, Thomas E Hall, Marco Morsch, Elinor Hortle, Alison Hogan, Sharron Chow, Serene S L Gwee, Jack J Stoddart, Garth Nicholson, Roger Chung, Nicholas J Cole
Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover effective therapeutics. We have generated a Tol2 Gateway-compatible toolbox to study neurodegenerative disorders in zebrafish, which includes promoters for astrocytes, microglia and motor neurons, multiple fluorophores, and compatibility for the introduction of genes of interest or disease-linked genes...
September 15, 2016: Zebrafish
Lisa A Schimmenti
No abstract text is available yet for this article.
December 2016: Zebrafish
Umur Önal, Chris Langdon
To optimize diet particle size for ingestion by zebrafish larvae, Danio rerio, a series of diet selection experiments were carried out using two different length classes: 5-day-old, first feeding larvae with a mean standard length (MLs) of 3.8 mm and 15-day-old larvae with MLs of 5.2 mm. For this purpose, crosslinked protein-walled capsules with five different size classes (≤20, 21-45, 46-75, 76-106, and 107-212 μm) were used in selection experiments. A particle size selection model was then developed after accounting for both rate of loss of capsules in the water column and their ingestion by larvae...
December 2016: Zebrafish
Doreen H Bartlett, Susan B Silk
In support of the new column in Zebrafish dedicated to initiating conversations about animal care policies and compliance for the zebrafish, the Office of Laboratory Animal Welfare (OLAW) has offered the following clarification and guidance.
December 2016: Zebrafish
Lloyd Hamilton, Katy R Astell, Gergana Velikova, Dirk Sieger
Glioblastoma multiforme is the most common and deadliest form of brain cancer. Glioblastomas are infiltrated by a high number of microglia, which promote tumor growth and surrounding tissue invasion. However, it is unclear how microglia and glioma cells physically interact and if there are differences, depending on glioma cell type. Hence, we have developed a novel live imaging assay to study microglia-glioma interactions in vivo in the zebrafish brain. We transplanted well-established human glioblastoma cell lines, U87 and U251, into transgenic zebrafish lines with labelled macrophages/microglia...
December 2016: Zebrafish
Louis W Wang, Inken G Huttner, Celine F Santiago, Diane Fatkin
No abstract text is available yet for this article.
December 2016: Zebrafish
Amina Lebcir, Rahma Hassan-Abdi, Constantin Yanicostas, Nadia Soussi-Yanicostas
No abstract text is available yet for this article.
December 2016: Zebrafish
Helena Fernandes, Helena Peres, António Paulo Carvalho
A standard diet for zebrafish, based on their specific nutritional requirements, is of primary importance to improve experimental outcomes with this model organism and optimize its large-scale production. However, the main basic nutritional requirements of zebrafish are yet to be determined. This study aimed at contributing to fill this gap by evaluating the dietary protein requirement of zebrafish juvenile. Ten isoenergetic fishmeal-based diets with increasing protein levels (15%-60%) were formulated, and each diet was assigned to duplicated groups of zebrafish (53...
December 2016: Zebrafish
Vasudev Kantae, Elke H J Krekels, Anita Ordas, Oskar González, Rob C van Wijk, Amy C Harms, Peter I Racz, Piet H van der Graaf, Herman P Spaink, Thomas Hankemeier
Zebrafish larvae (Danio rerio) are increasingly used to translate findings regarding drug efficacy and safety from in vitro-based assays to vertebrate species, including humans. However, the limited understanding of drug exposure in this species hampers its implementation in translational research. Using paracetamol as a paradigm compound, we present a novel method to characterize pharmacokinetic processes in zebrafish larvae, by combining sensitive bioanalytical methods and nonlinear mixed effects modeling...
December 2016: Zebrafish
Susana S Lopes, Martin Distel, Claudia Linker, Rita Fior, Rui Monteiro, Isaac H Bianco, Ruben Portugues, Uwe Strähle, Leonor Saúde
The European Zebrafish Principal Investigator Meeting (EZPM) is an ideal forum for group leaders using this fantastic animal model not only to discuss science but also to strengthen their interactions, to push forward technological advances, and to define guidelines for the use of this fish in research. The city of Lisbon (Portugal) was voted by the European group leaders to be the setting for the 4th EZPM, and the organizing committee, composed by Leonor Saúde (iMM Lisboa, PT), Susana Lopes (CEDOC, PT), Michael Orger (Champalimaud Foundation, PT), Rui Oliveira (ISPA, PT), and António Jacinto (CEDOC, PT), was very enthusiastic to organize a productive event...
December 2016: Zebrafish
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