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Nolan J Tiersch, William M Childress, Terrence R Tiersch
Vitrification is a method of cryopreservation that freezes samples rapidly, while forming an amorphous solid ("glass"), typically in small (μL) volumes. The goal of this project was to create, by three-dimensional (3D) printing, open vitrification devices based on an elliptical loop that could be efficiently used and stored. Vitrification efforts can benefit from the application of 3D printing, and to begin integration of this technology, we addressed four main variables: thermoplastic filament type, loop length, loop height, and method of loading...
May 16, 2018: Zebrafish
Hiroko Nakayama, Kan Katayama, Yukimasa Onabe, Atsushi Sato, Norihio Nishimura, Yasuhito Shimada
Rotifers are now becoming a standard live feed for first-feeding larvae, because of their easy rearing protocol and improved growth and survival rate, compared with other fish feed, paramecia, and processed powder fish feeds. In a group of rotifers, bdelloid rotifer, a freshwater plankton, exerts cryptobiosis (an ametabolic state of life) in response to desiccation and survived for a long time. Here, we created "dried rotifer sheet" for rearing larvae in their early developmental stage. More than 80% rotifers could survive after 18-month desiccation in the filter paper...
April 24, 2018: Zebrafish
John C Achenbach, Jessica Hill, Joseph P M Hui, Michael G Morash, Fabrice Berrue, Lee D Ellis
The Cannabis sativa plant contains numerous phytocannabinoids and terpenes with known or potential biological activity. For decades, plant breeders have been breeding the Cannabis plant to control for a desired ratio of the major cannabinoids. A high-throughput in vivo model to understand the relationship between the chemical composition of different strains and their therapeutic potential then becomes of value. Measuring changes in the behavioral patterns of zebrafish larvae is an established model with which to test the biological activity of neuroactive compounds...
April 10, 2018: Zebrafish
Mariane Gavazzoni, Leonardo M Paiz, Carlos A M Oliveira, Carla S Pavanelli, Weferson J Graça, Vladimir P Margarido
Astyanax is a species-rich polyphyletic genus distributed between the southern United States and central Argentina. The genus contains groups of cryptic species, which are difficult to distinguish, and are sometimes identified wrongly. Basic and molecular cytogenetic analyses were run on Astyanax abramis and three junior synonyms of Astyanax lacustris: Astyanax altiparanae, from the upper Paraná River basin, Astyanax asuncionensis, from the lower Paraná basin, and Astyanax jacuhiensis, from the upper Uruguay River...
April 10, 2018: Zebrafish
Li Zhou, Samwel Mchele Limbu, Fang Qiao, Zhen-Yu Du, Meiling Zhang
The use of antibiotics for anti-infection and growth promotion has caused the overuse of antibiotics in aquaculture. However, the benefit or risk of the long-term use of antibiotics on fish growth or health has not been fully addressed. In the present study, zebrafish were fed with sulfamethoxazole (SMX) or oxytetracycline (OTC) at the therapeutic concentrations (100 and 80 mg/kg body weight per day, respectively) for 6 weeks to mimic the long-term use of antibiotics. The digestive enzyme activities were higher in both antibiotic treatments, and higher oxygen consumption rate was found in OTC treated group...
April 2, 2018: Zebrafish
Arturo Vargas, Basilio Randazzo, Paola Riolo, Cristina Truzzi, Giorgia Gioacchini, Elisabetta Giorgini, Nino Loreto, Sara Ruschioni, Matteo Zarantoniello, Matteo Antonucci, Sara Polverini, Gloriana Cardinaletti, Simona Sabbatini, Francesca Tulli, Ike Olivotto
A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards. Due to minimal environmental impact, compared with most conventional feed commodities, insects deserve a growing attention as candidate ingredients for aquafeeds. The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing. Through a multidisciplinary approach, the major biological responses of fish to the new diets were assessed...
March 28, 2018: Zebrafish
Xiao-Yu Zhu, Si-Qi Wu, Sheng-Ya Guo, Hua Yang, Bo Xia, Ping Li, Chun-Qi Li
Heart failure is a leading cause of death and the development of effective and safe therapeutic agents for heart failure has been proven challenging. In this study, taking advantage of larval zebrafish, we developed a zebrafish heart failure model for drug screening and efficacy assessment. Zebrafish at 2 dpf (days postfertilization) were treated with verapamil at a concentration of 200 μM for 30 min, which were determined as optimum conditions for model development. Tested drugs were administered into zebrafish either by direct soaking or circulation microinjection...
March 20, 2018: Zebrafish
Himanshu Gaur, Narasimha Pullaguri, Shubham Nema, Srinithi Purushothaman, Yogesh Bhargava, Anamika Bhargava
For the assessment of cardiac function, heartbeat represents one key parameter. Current methods of heartbeat measurements in the zebrafish larvae usually require larval immobilization, fluorescent transgenic strains and a confocal microscope, costly commercial software for analysis, or strong programming skills if the software is open-source. Here, we present a simple yet powerful method of heartbeat analysis using untethered, unlabeled zebrafish larva using ImageJ (open-source software), which does not require programming skills...
March 13, 2018: Zebrafish
João P Morais-Silva, Alessandra V de Oliveira, Thomaz M C Fabrin, Nathália Alves Diamante, Sônia M A P Prioli, Augusto Frota, Weferson J da Graça, Alberto J Prioli
The current analysis investigates whether the uplift of the Serra da Esperança and the Ponta Grossa Arch in the Serra Geral resulted in ichthyofaunistic changes in adjacent basins. For this, we describe the phylogeographic structure among populations of Trichomycterus collected in hydrographic basins in southern Brazil by using partial nucleotide sequences of the mitochondrial gene Cytochrome C Oxidase subunit I. Analyses revealed that the nomenclature Trichomycterus davisi fails to contain the whole genetic diversity range found in the collected specimens and indicates at least six genetic lineages in Trichomycterus...
March 13, 2018: Zebrafish
Lucas Baumgärtner, Leonardo Marcel Paiz, Fabio Hiroshi Takagui, Roberto Laridondo Lui, Orlando Moreira Filho, Lucia Giuliano-Caetano, Ana Luiza de Brito Portela-Castro, Vladimir Pavan Margarido
Doradidae has been a target of phylogenetic studies over the last few years, but chromosomal information about the family is still scarce. Therefore, new cytogenetic data are provided herein and they are correlated with phylogenetic proposals to contribute to the knowledge of chromosomal evolution within doradids. Cytogenetic studies were performed on Trachydoras paraguayensis, Anadoras sp. "araguaia," Ossancora eigenmanni, Platydoras armatulus, and Rhinodoras dorbignyi. O. eigenmanni, P. armatulus, and R...
March 13, 2018: Zebrafish
Konrad Dabrowski, Mackenzie Miller
The current body of work on rearing larval/juvenile zebrafish is based on (1) utilization of freshwater and (2) diurnal light/dark cycle, (3) provision of live feed at modest density, and (4) culture in high visibility environment. We challenged these rearing approaches by maintaining zebrafish under constant light for 46-48 days (days postfertilization [dpf]), while securing continuous feeding in high turbidity and saline (1.8-2.1 parts per thousand) environment for the experiment's duration, allowing 24 h feeding/growth of fish from first exogenous feeding to maturation...
February 27, 2018: Zebrafish
Sebastien Andrew Rider, Finnius Austin Bruton, Richard George Collins, Bryan Ronald Conway, John James Mullins
Defects in the glomerular filtration barrier (GFB) play a major role in the onset of human renal diseases. Highly ramified glomerular cells named podocytes are a critical component of the GFB. Injury to podocytes results in abnormal excretion of plasma proteins, which can lead to chronic kidney disease. The conserved paired nephron of larval zebrafish is an excellent model for assessing glomerular function and injury. The efficacy of two known podocyte toxins was tested to refine models of acute podocyte injury in larval zebrafish...
February 26, 2018: Zebrafish
Tyrone J Tolbert, Shinnosuke Nakayama, Maurizio Porfiri
The advent of automated tracking software has significantly reduced the time required to record movement trajectories, thereby facilitating behavioral studies of zebrafish. However, results are substantially influenced by tracking errors, such as loss and misidentification of individuals. In this study, we present the development of an online citizen science platform, Tracking Nemo, to improve data accuracy on swimming trajectories of zebrafish groups. As an online extension of software for tracking the position of zebrafish from video recordings, Tracking Nemo offers volunteers the opportunity to contribute to science by manually correcting tracked trajectory data from their personal computers...
February 22, 2018: Zebrafish
Heather M Prior, Kerri Letwin, Amber Tuininga, Michelle Nguyen
We have developed a simple method for the induction of cataracts in adult zebrafish using hydrogen peroxide. Our method uses a corneal puncture followed by intraocular injection of hydrogen peroxide into the anterior chamber. The procedure can be done rapidly, forming a highly opaque cataract almost immediately, and fish recover quickly. The optical clarity of the lens is gradually regained over time, showing the innate ability of zebrafish to regenerate healthy lens tissue. This technique provides a simple in vivo system with which to assess mutant strains or targeted disruptions to genes expressed in the lens...
April 2018: Zebrafish
Rohit C Philip, Jeffrey J Rodriguez, Maki Niihori, Ross H Francis, Jordan A Mudery, Justin S Caskey, Elizabeth Krupinski, Abraham Jacob
Zebrafish have emerged as a powerful biological system for drug development against hearing loss. Zebrafish hair cells, contained within neuromasts along the lateral line, can be damaged with exposure to ototoxins, and therefore, pre-exposure to potentially otoprotective compounds can be a means of identifying promising new drug candidates. Unfortunately, anatomical assays of hair cell damage are typically low-throughput and labor intensive, requiring trained experts to manually score hair cell damage in fluorescence or confocal images...
April 2018: Zebrafish
Melissa L Fangmeier, Daniel W A Noble, Rose E O'Dea, Takuji Usui, Malgorzata Lagisz, Daniel Hesselson, Shinichi Nakagawa
An emergent field of animal personality necessitates a method for repeated high-throughput quantification of behavioral traits across contexts. In this study, we have developed an automated video stimulus approach to sequentially present different contexts relevant to five "personality" traits (exploration, boldness, neophobia, aggression, and sociability), successfully quantifying repeatable trait measurements in multiple individuals simultaneously. Although our method is designed to quantify personality traits in zebrafish, our approach can accommodate the quantification of other behaviors, and could be customized for other species...
April 2018: Zebrafish
Zu Soh, Motoki Matsuno, Masayuki Yoshida, Toshio Tsuji
Fear and anxiety in fish are generally evaluated by video-based behavioral analysis. However, it is difficult to distinguish the psychological state of fish exclusively through video analysis, particularly whether the fish are freezing, which represents typical fear behavior, or merely resting. We propose a system that can measure bioelectrical signals called ventilatory signals and simultaneously analyze swimming behavior in real time. Experimental results comparing the behavioral analysis of the proposed system and the camera system showed a low error level with an average absolute position error of 9...
April 2018: Zebrafish
Lauren J Norris, Virginia Watral, Michael L Kent
Cryopreservation is a common method used to preserve the sperm of various animal species, and it is widely used with zebrafish (Danio rerio). As with other animals, there is a possibility of paternal pathogen transmission through sperm. We evaluated the ability of five common and important pathogens of zebrafish to survive cryopreservation as used with zebrafish sperm and freezing without cryopreservant. We evaluated Mycobacterium chelonae, Mycobacterium marinum, and Edwardsiella ictaluri, each originally isolated from zebrafish, eggs of Pseuodocapillaria tomentosa, and spores of Pseudoloma neurophilia...
April 2018: Zebrafish
Rebecca Wilk, Naomi Ali, Samantha J England, Katharine E Lewis
Zebrafish are widely used as a model organism for research. Zebrafish embryos are also a useful resource for teaching students about vertebrate development. Here we describe a collaboration between two high school teachers and two university professors that used zebrafish to bring hands-on laboratory experiences to inner-city students, with the aim of increasing tangibility, and improving student understanding and retention, of several fundamental scientific concepts, such as the scientific method, cell division, mitosis, and Mendelian genetics...
April 2018: Zebrafish
Simone Rutz Costa, Robson Rabelo Velasques, Monique Tomazele Rovani, Marta Marques Souza, Juliana Zomer Sandrini
Fish cellular models are commonly used to study the toxic potential of environmentally relevant compounds. Several of these pollutants act on DNA and compromise its integrity. Little is known, however, about the DNA repair ability of these cellular models. Therefore, the aim of this study was to evaluate the DNA base excision repair (BER) of zebrafish Liver (ZF-L) cell line and primary hepatocytes. We performed kinetic studies of the DNA damage levels after exposure to hydrogen peroxide (H2 O2 , 20 μM for 10 min) using the Comet Assay...
April 2018: Zebrafish
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