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Juan E Franco-Restrepo, Diego A Forero, Rafael A Vargas
The analysis of behavior in animal models is an important objective in many research fields, including neuroscience, psychology, toxicology, and neuropsychopharmacology. Animal models have been used for many years, and several behavioral paradigms, such as locomotor activity, social interactions, and cognitive behavior, have been studied in animal models to correlate the behaviors with pharmacological or environmental interventions and with molecular, biochemical, and physiological findings. We reviewed the literature looking for open-source, freely available software to analyze animal behavior and found 12 freely available programs: ToxTrack, EthoWatcher, Mouse Behavior Tracker, Mouse Move, JAABA, wrMTrck, AnimalTracker, idTracker, Ctrax, Mousetracker, VideoHacking, and Cowlog, which were developed with different programs, work on different platforms, and have particular types of inputs and outputs and analysis capabilities...
January 9, 2019: Zebrafish
Jose González-Fraga, Victor Dipp-Alvarez, Ulises Bardullas
Recently, the evaluation of spontaneous tail movement (STM) in zebrafish embryos has been proposed as a possible neurotoxicity marker in studies on behavioral changes due to chemical exposure. Currently, automated STM quantification can only be performed using high-cost commercial software. We have developed a simple and reliable MATLAB® script called ZebraSTM that allows the automatic analysis of STM in multiple zebrafish embryos.
January 7, 2019: Zebrafish
Rafael A Lara, Raquel O Vasconcelos
Zebrafish is a well-established model organism in hearing research. Although the acoustic environment is known to shape the structure and sensitivity of auditory systems, there is no information on the natural soundscape of this species. Moreover, zebrafish are typically reared in large-scale housing systems (HS), although their acoustic properties and potential effects on hearing remain unknown. We characterized the soundscape of both zebrafish natural habitats and laboratory captive conditions, and discussed possible impact on auditory sensitivity...
December 26, 2018: Zebrafish
Mustafa Hamimi, Mitra Khabooshan, Hozana A Castillo, Jan Kaslin
Gene editing using clustered regularly interspaced short palindromic repeats (CRISPR) is widely used throughout the zebrafish community for the generation of knockouts and knockins. One of the bottlenecks that exists during the process is the laborious screening of injected embryos for F0 founder fish or CRISPants, weeks after the injection date. In this study we show that the use of fluorescently tagged tracrRNA and sorting for fluorescent embryos as early as the 512-cell stage using stereomicroscope significantly improve yield of fish with successfully CRISPR/Cas9-edited genomes...
December 26, 2018: Zebrafish
Gil Martins, Patrícia Diogo, Wilson Pinto, Paulo J Gavaia
Zebrafish is a model species with a high variability of feeding regimes among fish facilities. The use of live feeds for early life stages is a common practice, and few studies have focused early weaning into microdiets. The lack of standardized feeding protocols among research facilities promotes discrepancies in biological performances, and few studies relate dietary regimes to zebrafish development. The objective of this work was to assess the effect of an early transition into microdiets in zebrafish development by evaluating growth, survival, reproductive performance, and skeletal anomalies...
December 18, 2018: Zebrafish
Jonathan Pena Castro, Ricardo Shohei Hattori, Túlio Teruo Yoshinaga, Duílio Mazzoni Zerbinato de Adrade Silva, Fausto Foresti, Mateus Henrique Santos, Mara Cristina Almeida, Roberto Ferreira Artoni
Astyanax is an abundant fish genus in South America. Some species of this group are characterized by the presence of B chromosomes and absence of morphologically differentiated sex chromosomes. In this study, we used quantitative real-time polymerase chain reaction to characterize mRNA expression of dmrt1 in Astyanax scabripinnis gonads. Maturing gonads of males with the B chromosome overexpressed dmrt1. Our findings suggest that B chromosomes may have an adaptive role in A. scabripinnis sex determination and maintenance...
December 18, 2018: Zebrafish
Aldana P David, Brenda Delfín, Magela Rodao, Roberto Rodríguez-Ortiz
The V Latin American Zebrafish Network Course and Symposium was held from May 4 to 12, 2018 at the Institute of Biotechnology of the National Autonomous University of Mexico. A group of 19 students from eight different countries were selected and trained during a week in a variety of topics and techniques using zebrafish as a model, followed by a 2-day symposium. In this article, we want to point out not only the outstanding activities carried out during the course but also highlight the great experience, the knowledge, and the fantastic relationships we gained from those days...
December 4, 2018: Zebrafish
Patrícia Diogo, Gil Martins, Ana Eufrásio, Tomé Silva, Elsa Cabrita, Paulo Gavaia
Selection criteria for sperm cryopreservation are highly relevant in zebrafish since sperm quality is particularly variable in this species. Successful cryopreservation depends on high-quality sperm, which can only be ensured by the selection of breeders. Consequently, male selection and management are a priority to improve cryopreservation, and therefore, this study aimed to characterize optimal age and sperm collection frequency in zebrafish. For this purpose, males from wild type (AB) and from a transgenic line [Tg(runx2:eGFP)] were sampled at 6, 8, 12, and 14 months...
December 4, 2018: Zebrafish
Hemagowri Venkatasubramanian, Velanganni Selvaraj, Mohankumar Ramasamy, Kirankumar Santhakumar
Induction of all-male population in Siamese fighting fish, Betta splendens, has potential application in ornamental fish trade. In addition, the sexually dimorphic nature of aggressive behavior exhibited by this species has made it into an emerging model for behavioral studies. Fadrozole, an aromatase inhibitor, which has been used widely in masculinization, has captivated us to use it in this study. Twenty one days postfertilization (dpf), B. splendens fry were subjected to discrete immersion treatment with various concentrations of fadrozole, and eventually, analyses of various socioreproductive behaviors and analyses of stress markers such as dopamine in brain samples, sex hormones, cortisol, and glucose in plasma samples were performed...
December 4, 2018: Zebrafish
Ruilin Zhang, Jie He, Xu Wang, Weijun Pan, Ying Cao, Yao Zu, Qiu Jiang, Jiulin Du, Tao P Zhong
The fifth zebrafish research conference of China was held from October 29 to November 1, 2017, at Wuzhen, China, a beautiful small town that is close to Shanghai. The organizing committee, composed of Tao P. Zhong, Ruilin Zhang, Jie He, Xu Wang, Weijun Pan, Ying Cao, Yao Zu, Qiu Jiang, and Jiulin Du, organized enthusiastically and successfully the 4-day meeting. A total of 646 attendees from 110 academic and corporate institutions, mainly from China and also United States, Germany, Sweden, Singapore, Taiwan, and Japan, presented and discussed their recent research progresses...
November 27, 2018: Zebrafish
Seyed-Mohammadreza Samaee, Nafiseh Manteghi, Alicia Estévez
Sperm quality is an important topic in general health, chemotherapy, and gamete preservation technology. Fatty acid (FA) composition of membranes, which is influenced by the diet, plays key roles in sperm biology and quality. Dietary supplementation with natural products can be used as a technique to screen potential agents to protect, modify, and recover sperm quality. In this study, zebrafish (male [♂-ZF] and female [♀-ZF]) were fed a single cultivar olive oil (OO) bioencapsulated in Artemia. OO-treated ♂-ZF had higher (p < 0...
November 20, 2018: Zebrafish
Marina S Cunha, Aline R Fregonezi, Lucioni Fava, Alexandre W S Hilsdorf, Lucio A O Campos, Jorge A Dergam
Astyanax bimaculatus, a ubiquitous species in many Neotropical basins, is characterized by a complex taxonomy and are currently considered a species complex. The goal of this study was to analyze 31 populations (N = 136) of this species from southeastern Brazil using cytogenetic techniques: conventional staining, nucleolar organizer region (Ag-NOR), C-banding, and 18S and 5S recombinant DNA fluorescent in situ hybridization (FISH) probes; and molecular techniques: S72, RAG2, and COI. All populations were 2n = 50 (6m + 20sm +18st +6a); Ag-NORs were predominantly simple, C-banding revealed high variation levels within and among basins, and the FISH probes 18S and 5S were restricted to chromosome pairs 14 and 7, respectively...
November 20, 2018: Zebrafish
Cristhian Camilo Conde-Saldaña, Marina Souza Cunha, Juan Gabriel Albornoz-Garzón, Cynthia Aparecida Valiati Barreto, Nicole Ibagón, Francisco Antonio Villa-Navarro, Jorge Abdala Dergam
The monotypic Astroblepidae fish family includes 81 species distributed along Central and Northern Andes in South America and Panamá in Central America; most aspects regarding its biology, taxonomy, and chromosomal features remain largely unknown. This study reports the karyotype of two sympatric Astroblepus species from the Colombian Andes, aiming to provide novel information on karyotype organization and reveal possible chromosomal rearrangements occurred on these species, through mapping of different repetitive DNA classes, including microsatellites and ribosomal DNA multigene families...
November 20, 2018: Zebrafish
Timo Friedrich, Alon M Douek, Celia Vandestadt, Donald Wlodkowic, Jan Kaslin
Zebrafish larvae are suitable in vivo models for toxicological and pharmacological screens due to their transparency, small size, ex utero development, and genetic and physiological similarity to humans. Using modern imaging techniques, cells and tissues can be dynamically visualized over several days in multiple zebrafish larvae. However, precise specimen immobilization and maintenance of homeostatic conditions remain a challenge for longitudinal studies. A highly customizable mounting configuration with inbuilt means of controlling temperature and media flow would therefore be a valuable tool to facilitate long-term imaging of a large number of specimens...
November 13, 2018: Zebrafish
Lindsay A Holden, Charles Wilson, Zachary Heineman, Kimberly P Dobrinski, Kim H Brown
Zebrafish (Danio rerio) are a widely utilized model system for human disorders, but common laboratory strains have distinct behavioral and physiological differences. Accompanying these known strain differences, commonly used "wildtype" zebrafish strains have both shared and unique suites of single nucleotide polymorphisms and copy number variants (CNVs). Despite this, genomic variation is often ignored in study design, and the actual strain used is often not adequately reported. The goal of this study was to assess CNVs across three common laboratory strains of zebrafish-AB, Tubingen (TU), and WIK-and provide these data as a tool for the zebrafish community...
November 10, 2018: Zebrafish
Katherine J Robinson, Kristy C Yuan, Emily K Don, Alison L Hogan, Claire G Winnick, Madelaine C Tym, Caitlin W Lucas, Hamideh Shahheydari, Maxinne Watchon, Ian P Blair, Julie D Atkin, Garth A Nicholson, Nicholas J Cole, Angela S Laird
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive loss of motor neurons. ALS can be modeled in zebrafish (Danio rerio) through the expression of human ALS-causing genes, such as superoxide dismutase 1 (SOD1). Overexpression of mutated human SOD1 protein causes aberrant branching and shortening of spinal motor axons. Despite this, the functional relevance of this axon morphology remains elusive. Our aim was to determine whether this motor axonopathy is correlated with impaired movement in mutant (MT) SOD1-expressing zebrafish...
October 27, 2018: Zebrafish
Anita Rácz, Toni Dwyer, Shaun S Killen
In 2017, the zebrafish unit at University of Glasgow experienced a detrimental outbreak of pathogenic bacterium, Mycobacterium haemophilum. The presence of other bacterial species was also confirmed by bacteriology growth in the same unit. The affected individuals composed of a wild-origin parental population sourced from India and their F1 offspring generation. Bacteria were diagnostically confirmed to be present systemically in fish and within the water and biofilm of the recirculating zebrafish system. In the absence of a publicly accessible step-by-step disinfectant protocol for these difficult-to-eliminate pathogens, we devised a successful procedure to eradicate mycobacteria and Aeromonas species after colony removal using Cleanline Chlorine tablets (active ingredient Sodium dichloroisocyanurate) and Virkon Aquatic® ...
October 24, 2018: Zebrafish
Diovani Piscor, Allan Pierre Bonetti Pozzobon, Carlos Alexandre Fernandes, Liano Centofante, Patricia Pasquali Parise-Maltempi
Astyanax is a genus with a wide distribution ranging from the south United States to north of Patagonia (Argentina). The available cytogenetic data on Astyanax indicate a high karyotypic diversity, with diploid number of 36-52 chromosomes, presence of B chromosomes, heterochromatin polymorphism, and variations with respect to the number and localization of nucleolar organizer regions (NORs) and 18S and 5S ribosomal DNA sites. In the present study, we estimated the evolutionary history and times of divergence for 10 nominal Astyanax species from the South and Central/North American (Cna) continents, which present distinct chromosomal characteristics, based on molecular clocks inferred from mitochondrial DNA sequence...
October 24, 2018: Zebrafish
Zoltan M Varga, Stephen C Ekker, Christian Lawrence
Current standards for husbandry and maintenance of zebrafish and other aquatic species in the laboratory are diverse, and are subject to laboratory performance, engineering, and practice standards (the Guide), institutional interpretation, national animal welfare laws, and cultural differences. Consequently, it is difficult, and probably not advantageous, to establish a single standard in view of the hardy nature of zebrafish and the diversity of research requirements it is used to address. Based on their natural habitat, zebrafish can thrive in a variety of environmental conditions, which is a specific advantage for working with this laboratory organism...
December 2018: Zebrafish
Anthony J Treichel, Jacob H Hines
During vertebrate neural development, oligodendrocytes insulate nerve axons with myelin sheaths. Zebrafish (Danio rerio) has emerged as a useful model organism for studying oligodendrocyte development. However, the absence of an in vitro culture system necessitates in vivo manipulations and analyses, which, in some instances, limits the questions that can be addressed. To fill this gap we developed a mixed coculture system for embryonic zebrafish neurons and oligodendrocyte-lineage cells. Cultures harvested from embryos ≥30 hours postfertilization (hpf) yielded oligodendrocyte progenitor cells (OPCs) positive for olig2 and sox10 transgenic reporters...
December 2018: Zebrafish
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