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Zebrafish

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https://www.readbyqxmd.com/read/28737485/risk-taking-during-feeding-between-and-within-population-variation-and-repeatability-across-contexts-among-wild-zebrafish
#1
Tamal Roy, Rohitashva Shukla, Anuradha Bhat
Behavioral responses are shaped by a complex interplay of extrinsic factors, such as predation and flow conditions, and intrinsic properties such as metabolic state. We investigated variations in boldness among four populations of zebrafish collected from widely varying habitat conditions across India. We measured feeding latencies of fish in four contexts: in a novel environment; in an environment with refuge and predators; in the presence of a shoal; and in the presence of a shoal and predators. The population from a high-flow and high-predation habitat was significantly bolder than populations from low-predation and low-flow/stagnant habitats...
July 24, 2017: Zebrafish
https://www.readbyqxmd.com/read/28727940/intestinal-transit-time-and-cortisol-mediated-stress-in-zebrafish
#2
Clayton Brady, Maxwell Denora, Ian Shannon, Karl J Clark, Adam Rich
Intestinal motility, the spontaneous and rhythmic smooth muscle contraction, is a complex process that is regulated by overlapping and redundant regulatory mechanisms. Primary regulators intrinsic to the gastrointestinal tract include interstitial cells of Cajal, enteric neurons, and smooth muscle cells. Extrinsic primary regulators include the autonomic nervous system, immune system, and the endocrine system. Due to this complexity, a reductionist approach may be inappropriate if the ultimate goal is to understand motility regulation in vivo...
July 20, 2017: Zebrafish
https://www.readbyqxmd.com/read/28714785/effects-of-alcohol-on-inhibitory-avoidance-learning-in-zebrafish-danio-rerio
#3
Ricardo Rodrigues Amorim, Priscila Fernandes Silva, Ana Carolina Luchiari
The zebrafish (Danio rerio) can be used in studies addressing the effects of drugs on learning, memory, and anxiety. In the present study, we investigated the effect of different alcohol treatments (chronic and acute) on the learning and anxiety response of zebrafish in an inhibitory avoidance paradigm. Zebrafish were initially exposed to different alcohol treatments and submitted to an inhibitory avoidance protocol, where an electroshock was applied to the fish as they swam from the white to the black side of a shuttle box tank (naturally preferred environment of zebrafish)...
July 17, 2017: Zebrafish
https://www.readbyqxmd.com/read/28704145/adult-zebrafish-danio-rerio-an-alternative-behavioral-model-of-formalin-induced-nociception
#4
Francisco Ernani Alves Magalhães, Caio Átila Prata Bezerra de Sousa, Sacha Aubrey Alves Rodrigues Santos, Renata Barbosa Menezes, Francisco Lucas Alves Batista, Ângela Oliveira Abreu, Messias Vital de Oliveira, Luiz Francisco Wemmenson Gonçalves Moura, Ramon da Silva Raposo, Adriana Rolim Campos
The zebrafish (Danio rerio) has been proposed as a low-cost and simple alternative to the use of higher vertebrates in laboratory research on novel compounds with antinociceptive potential. In this study, we tested adult zebrafish (Danio rerio) as an alternative behavioral model of formalin-induced nociception. We evaluated the nociceptive effect of 0.1% formalin (3 or 5 μL; intramuscularly [i.m.]), applied into the tail or lips, on locomotor activity, using as parameter the number of times the fish crossed the lines between the quadrants of a glass Petri dish during the neurogenic stage (0-5 min) and the inflammatory stage (15-30 min)...
July 13, 2017: Zebrafish
https://www.readbyqxmd.com/read/28678656/a-simple-and-inexpensive-microinjection-system-for-zebrafish-embryos-and-larvae
#5
Seyed-Mohammadreza Samaee, Hanieh Nikkhah, Zoltan M Varga, Bahman Rezaei
Microinjection is a widely used technique to inject defined volumes and concentrations of substances and explore their physiological function in vivo. The technique has been particularly successful with zebrafish embryos; however, the injection equipment can be relatively expensive and therefore available only to well-funded laboratories. In this study, a simple, cheap, easy-to-assemble, and easy-to-use setup with a straightforward, accurate, and efficacious calibration method is introduced. The accuracy of this calibration method was tested by comparing with the results of calibration methods that are currently used in high-cost systems...
July 5, 2017: Zebrafish
https://www.readbyqxmd.com/read/28678643/a-novel-photocatalytic-purification-system-for-fish-culture
#6
Basilio Randazzo, Giulia Chemello, Ivan Tortarolo, Gian Luca Chiarello, Maciej Zalas, Alessandra Santini, Margaret Liberatore, Marco Liberatore, Elena Selli, Ike Olivotto
The accumulation of nitrogen compounds represents a pivotal problem in the management of fish culture. Several methods were investigated in the last decades for treatment of waste waters, and the use of photocatalytic materials has received increasing attention. The photocatalytic degradation (PCD) process with titanium dioxide (TiO2) represents the most promising single-step method to promote the removal of nitrogen compounds from water. The present study compares for the first time the effects on fish culture of a classical mechanical, biological, and ultraviolet purification system to a TiO2-PCD one, with particular emphasis on water chemistry and on physiological responses in zebrafish...
July 5, 2017: Zebrafish
https://www.readbyqxmd.com/read/28654369/gymnotus-coatesi-gymnotiformes-a-case-of-colocation-of-multiple-sites-of-18s-rdna-with-telomeric-sequences
#7
Milla de Andrade Machado, Adauto Lima Cardoso, Susana Suely Rodrigues Milhomem-Paixão, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi
Gymnotus coatesi is a small and rare species of banded knife fish that was originally described by LaMonte in 1935, found along the main stretch of the Amazon River. There is no described cytogenetic data on this species. We analyzed the karyotype of five specimens of G. coatesi collected from Cururutuia Stream in Bragança, Pará, Brazil. The obtained diploid number is 50 and the karyotypic formula is 24 m/sm +26 st/a. The constitutive heterochromatin is DAPI positive and distributed mainly in the centromeric and pericentromeric regions of the chromosomes...
June 27, 2017: Zebrafish
https://www.readbyqxmd.com/read/28654351/zz-z0-the-new-system-of-sex-chromosomes-in-eigenmannia-aff-trilineata-teleostei-gymnotiformes-sternopygidae-characterized-by-molecular-cytogenetics-and-dna-barcoding
#8
Cristian Araya-Jaime, Nadayca T Bonani Mateussi, Ricardo Utsunomia, Guilherme J Costa-Silva, Claudio Oliveira, Fausto Foresti
The cytogenetic characteristics of Eigenmannia aff. trilineata were analyzed by basic and molecular cytogenetics, applying fluorescent in situ hybridization, with 18S and 5S rDNA and U2 snRNA probes. The species revealed a kind of polymorphism associated to ZZ/Z0 type sex chromosomes, with 2n = 32 (8m+2sm+22a, NF = 42) in all males under analysis, whereas females evidenced 2n = 31 (8m+1sm+22a, NF = 40). C-banding showed constitutive heterochromatin restricted to the pericentromeric region of all chromosomes and single-nucleolus organized regions on pair 11...
June 27, 2017: Zebrafish
https://www.readbyqxmd.com/read/28570122/simufish-an-interactive-application-to-teach-k-12-students-about-zebrafish-behavior
#9
Violet Mwaffo, Veronika Korneyeva, Maurizio Porfiri
As the zebrafish is rapidly becoming a species of choice in preclinical research, several efforts are being placed toward creating educational programs for K-12 students based on this promising model organism. However, as any other model organisms, the use of zebrafish in classroom settings requires additional experimental resources and poses ethical challenges related to animal use. To mitigate these factors, we have developed an application (app), simUfish, which implements a mathematical model of zebrafish behavior for generating multiple fish trajectories and animating their body undulations...
June 1, 2017: Zebrafish
https://www.readbyqxmd.com/read/28557696/comparative-cytogenetics-of-the-black-ghost-knifefish-gymnotiformes-apteronotidae-evidence-of-chromosomal-fusion-and-pericentric-inversions-in-karyotypes-of-two-apteronotus-species
#10
Carlos Alexandre Fernandes, Leonardo Marcel Paiz, Lucas Baumgärtner, Vladimir Pavan Margarido, Margarida Maria de Rossi Vieira
The karyotype and chromosomal characteristics of Apteronotus albifrons and Apteronotus caudimaculosus collected from populations of two different large Brazilian river basins were analyzed by conventional and molecular cytogenetic techniques, to contribute to the differentiation and identification of the species in this genus. The diploid chromosome number was 2n = 24 for A. albifrons, but with difference in the karyotype structure and fundamental number values between two populations under study. In A...
May 30, 2017: Zebrafish
https://www.readbyqxmd.com/read/28609254/chromosomal-diversity-of-three-species-of-hypostomus-lac%C3%A3-p%C3%A3-de-1803-siluriformes-loricariidae-from-the-paran%C3%A3-river-basin-brazil-a-species-complex-in-hypostomus-ancistroides-reinforced-by-a-zz-zw-sex-chromosome-system
#11
Marcia Cristina de Souza Lara Kamei, Lucas Baumgärtner, Suzana Paiva, Cláudio Henrique Zawadzki, Isabel Cristina Martins-Santos, Ana Luiza de Brito Portela-Castro
Hypostomus shows wide morphological patterns, corroborated by great chromosomal diversity that has suggested the existence of new species, especially from small tributaries. Cytogenetic analysis has contributed to estimate a cryptic diversity providing important data for taxonomic and evolutionary studies. Cytogenetic techniques were carried out on species from a small tributary of Ivaí River, Keller River (upper Paraná River basin): Hypostomus aff. ancistroides, Hypostomus topavae, and Hypostomus aff. hermanni that presented 2n = 68, 80, and 72 chromosomes, respectively...
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28570143/zebrafish-and-the-cancer-moonshot
#12
Lisa A Schimmenti
No abstract text is available yet for this article.
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28557653/efficient-transduction-of-zebrafish-melanoma-cell-lines-and-embryos-using-lentiviral-vectors
#13
Maurizio Fazio, Serine Avagyan, Ellen van Rooijen, William Mannherz, Charles K Kaufman, Riadh Lobbardi, David M Langenau, Leonard I Zon
The establishment of in vitro cultures of zebrafish cancer cells has expanded the potential of zebrafish as a disease model. However, the lack of effective methods for gene delivery and genetic manipulation has limited the experimental applications of these cultures. To overcome this barrier, we tested and optimized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviral and retroviral vector transduction protocols. We show that lentivirus successfully and efficiently transduced zebrafish melanoma cell lines in vitro, allowing antibiotic selection, fluorescence-based sorting, and in vivo allotransplantation...
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28537503/open-rac-open-design-recirculating-and-auto-cleaning-zebrafish-maintenance-system
#14
Shubham Nema, Yogesh Bhargava
Zebrafish is a vertebrate animal model. Their maintenance in large number under laboratory conditions is a daunting task. Commercially available recirculating zebrafish maintenance systems are used to efficiently handle the tasks of automatic sediment cleaning from zebrafish tanks with minimal waste of water. Due to their compact nature, they also ensure the maximal use of available lab space. However, the high costs of commercial systems present a limitation to researchers with limited funds. A cost-effective zebrafish maintenance system with major features offered by commercially available systems is highly desirable...
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28520533/a-fully-automated-high-throughput-zebrafish-behavioral-ototoxicity-assay
#15
Douglas W Todd, Rohit C Philip, Maki Niihori, Ryan A Ringle, Kelsey R Coyle, Sobia F Zehri, Leanne Zabala, Jordan A Mudery, Ross H Francis, Jeffrey J Rodriguez, Abraham Jacob
Zebrafish animal models lend themselves to behavioral assays that can facilitate rapid screening of ototoxic, otoprotective, and otoregenerative drugs. Structurally similar to human inner ear hair cells, the mechanosensory hair cells on their lateral line allow the zebrafish to sense water flow and orient head-to-current in a behavior called rheotaxis. This rheotaxis behavior deteriorates in a dose-dependent manner with increased exposure to the ototoxin cisplatin, thereby establishing itself as an excellent biomarker for anatomic damage to lateral line hair cells...
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28488936/long-term-social-recognition-memory-in-zebrafish
#16
Natália Madeira, Rui F Oliveira
In species in which individuals live in stable social groups, individual recognition is expected to evolve to allow individuals to remember past interactions with different individuals and adjust future behavior toward them accordingly. Thus, social memory is expected to be a ubiquitous component of social cognition of social species. However, few studies have investigated the occurrence of social memory in non-mammals. Here we evaluated the ability of zebrafish (Danio rerio) to recognize different conspecifics and to retain this information in long lasting (i...
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28488934/the-zebrafish-curly-fry-is-required-for-proper-centrosome-and-mitotic-spindle-assembly
#17
Mi Hye Song, Jeffrey C Medley, John Y Kuwada
The zebrafish curly fry (cfy) mutation leads to a dramatic increase in mitotic index and cell death starting during neural tube formation. The mutant phenotype is cell autonomous and does not result from defects in apical/basal polarity within the neuroepithelium. The increase in mitotic index could be due to increased proliferation or cell cycle arrest in mitosis. cfy embryos were analyzed to examine these two possibilities. By labeling embryos with a pulse of BrdU and anti-phospho-histone 3 and examining the DNA content by fluorescence activated cell sorting, we show that cfy mutants exhibit no increase in proliferation, but a significant increase in the number of cells arrested in mitosis...
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28488933/zebrafish-as-a-screening-model-for-testing-the-permeability-of-blood-brain-barrier-to-small-molecules
#18
Seong Soon Kim, So Hee Im, Jung Yoon Yang, Yu-Ri Lee, Geum Ran Kim, Jin Sil Chae, Dae-Seop Shin, Jin Sook Song, Sunjoo Ahn, Byung Hoi Lee, Jae Chun Woo, Jin Hee Ahn, Chang Soo Yun, Phiho Kim, Hyoung Rae Kim, Kyeong-Ryoon Lee, Myung Ae Bae
The objective of this study was to evaluate the permeability of small molecules into the brain via the blood-brain barrier in zebrafish and to investigate the possibility of using this animal model as a screening tool during the early stages of drug discovery. Fifteen compounds were used to understand the permeation into the brain in zebrafish and mice. The ratio of brain-to-plasma concentration was compared between the two animal models. The partition coefficient (Kp,brain), estimated using the concentration ratio at designated times (0...
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28422577/enrichment-of-zebrafish-danio-rerio-hamilton-1822-diet-with-polyunsaturated-fatty-acids-improves-fecundity-and-larvae-quality
#19
Joanna Nowosad, Dariusz Kucharczyk, Katarzyna Targońska
The zebrafish is a freshwater fish of the Cyprinidae family, which is frequently used in scientific research. It owes its popularity to its genome, whose structure is comparable to the human genome and, for this reason, this species is often used in human medical research. However, such research requires high-quality material to conduct tests producing repeatable results. This study examines the effect of providing feed enriched with essential fatty acids, especially docosahexaenoic acid, to zebrafish spawners on fertility as well as the survival rate and growth of their offspring...
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28394244/a-zebrafish-model-of-human-fibrodysplasia-ossificans-progressiva
#20
Melissa LaBonty, Nicholas Pray, Pamela C Yelick
Fibrodysplasia ossificans progressiva (FOP) is a rare, autosomal dominant genetic disorder in humans characterized by explosive inflammatory response to injury leading to gradual ossification within fibrous tissues, including skeletal muscle, tendons, and ligaments. A variety of animal models are needed to study and understand the etiology of human FOP. To address this need, here we present characterizations of the first adult zebrafish model for FOP. In humans, activating mutations in the Type I BMP/TGFβ family member receptor, ACVR1, are associated with FOP...
August 2017: Zebrafish
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