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David Kimelman
In June 2018, I traveled to India to teach in a Tibetan Buddhist monastery under the auspices of the Emory-Tibet Science Initiative (ETSI), a program that brings aspects of science education to the three major Tibetan monastic universities in exile. My role was to teach developmental biology to the monks over a 9-day period, and I found zebrafish development to be an excellent vehicle for introducing them to both the wonder of embryonic development and to some of the most advanced findings in the field of developmental biology...
October 31, 2018: Zebrafish
Katherine J Robinson, Kristy C Yuan, Emily K Don, Alison L Hogan, Claire G Winnick, Madelaine C Tym, Caitlin W Lucas, Hamideh Shahheydari, Maxinne Watchon, Ian P Blair, Julie D Atkin, Garth A Nicholson, Nicholas J Cole, Angela S Laird
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive loss of motor neurons. ALS can be modeled in zebrafish (Danio rerio) through the expression of human ALS-causing genes, such as superoxide dismutase 1 (SOD1). Overexpression of mutated human SOD1 protein causes aberrant branching and shortening of spinal motor axons. Despite this, the functional relevance of this axon morphology remains elusive. Our aim was to determine whether this motor axonopathy is correlated with impaired movement in mutant (MT) SOD1-expressing zebrafish...
October 27, 2018: Zebrafish
Anita Rácz, Toni Dwyer, Shaun S Killen
In 2017, the zebrafish unit at University of Glasgow experienced a detrimental outbreak of pathogenic bacterium, Mycobacterium haemophilum. The presence of other bacterial species was also confirmed by bacteriology growth in the same unit. The affected individuals composed of a wild-origin parental population sourced from India and their F1 offspring generation. Bacteria were diagnostically confirmed to be present systemically in fish and within the water and biofilm of the recirculating zebrafish system. In the absence of a publicly accessible step-by-step disinfectant protocol for these difficult-to-eliminate pathogens, we devised a successful procedure to eradicate mycobacteria and Aeromonas species after colony removal using Cleanline Chlorine tablets (active ingredient Sodium dichloroisocyanurate) and Virkon Aquatic® ...
October 24, 2018: Zebrafish
Diovani Piscor, Allan Pierre Bonetti Pozzobon, Carlos Alexandre Fernandes, Liano Centofante, Patricia Pasquali Parise-Maltempi
Astyanax is a genus with a wide distribution ranging from the south United States to north of Patagonia (Argentina). The available cytogenetic data on Astyanax indicate a high karyotypic diversity, with diploid number of 36-52 chromosomes, presence of B chromosomes, heterochromatin polymorphism, and variations with respect to the number and localization of nucleolar organizer regions (NORs) and 18S and 5S ribosomal DNA sites. In the present study, we estimated the evolutionary history and times of divergence for 10 nominal Astyanax species from the South and Central/North American (Cna) continents, which present distinct chromosomal characteristics, based on molecular clocks inferred from mitochondrial DNA sequence...
October 24, 2018: Zebrafish
Rachele Macirella, Manuela Tripepi, Elvira Brunelli
In agroecosystems, a variety of pesticides may enter aquatic habitats exerting a number of toxic effects on living organisms. Due to their wide use and the repeated application, fungicides can be found at a much greater concentration than other agrochemicals in all environmental compartments. One of these fungicides, tebuconazole (TBZ), has been recognized as an endocrine-disrupting chemical, and the available literature focuses mainly on this mechanism of action. In the present study by subjecting the zebrafish model to an environmentally realistic concentration of TBZ, we clearly demonstrate that this fungicide has the potential to induce severe morphological and ultrastructural alterations in gills...
October 17, 2018: Zebrafish
Nobuyuki Shimizu, Miho Matsuda
Testicular tumors are the most common solid malignant tumors in men 20-35 years of age. Although most of testicular tumors are curable, current treatments still fail in 15%-20% of patients. However, insufficient understanding of the molecular basis and lack of animal models limit development of more effective treatments. This study reports the identification of a novel zebrafish mutant line, ns1402, which develops testicular germ cell tumors (TGCTs). While both male and female ns1402 mutants were fertile at young age, male ns1402 mutants became infertile as early as 9 months of age...
October 9, 2018: Zebrafish
Anthony J Treichel, Jacob H Hines
During vertebrate neural development, oligodendrocytes insulate nerve axons with myelin sheaths. Zebrafish (Danio rerio) has emerged as a useful model organism for studying oligodendrocyte development. However, the absence of an in vitro culture system necessitates in vivo manipulations and analyses, which, in some instances, limits the questions that can be addressed. To fill this gap we developed a mixed coculture system for embryonic zebrafish neurons and oligodendrocyte-lineage cells. Cultures harvested from embryos ≥30 hours postfertilization (hpf) yielded oligodendrocyte progenitor cells (OPCs) positive for olig2 and sox10 transgenic reporters...
October 9, 2018: Zebrafish
Ralph Slijkerman, Alexander Goloborodko, Sanne Broekman, Erik de Vrieze, Lisette Hetterschijt, Theo Peters, Milou Gerits, Hannie Kremer, Erwin van Wijk
The frequent deep-intronic c.7595-2144A>G mutation in intron 40 of USH2A generates a high-quality splice donor site, resulting in the incorporation of a pseudoexon (PE40) into the mature transcript that is predicted to prematurely terminate usherin translation. Aberrant USH2A pre-mRNA splicing could be corrected in patient-derived fibroblasts using antisense oligonucleotides. With the aim to study the effect of the c.7595-2144A>G mutation and USH2A splice redirection on retinal function, a humanized zebrafish knockin model was generated, in which 670 basepairs of ush2a intron 40 were exchanged for 557 basepairs of the corresponding human sequence using an optimized CRISPR/Cas9-based protocol...
October 3, 2018: Zebrafish
Chongjun Zhao, Erwen Li, Zhaoyi Wang, Jinghuan Tian, Yihang Dai, Yuanyuan Ni, Farong Li, Zhiqiang Ma, Ruichao Lin
Zebrafish larvae were used to further understand the liver toxicity of nux vomica. The histopathology, protein expression, and gene expression were assessed to confirm apoptosis in the liver, and then, profiles of the metabolites in zebrafish were investigated by untargeted metabolomic assessment to understand the potential toxicity mechanism of nux vomica. Histopathological observations showed that nux vomica caused damage to liver cells. Western blot results indicated increased expression of activated caspase3, and the result of real-time polymerase chain reaction showed a significant increase in the expression level of caspase-3, caspase-8, and caspase-9 genes (p < 0...
October 2, 2018: Zebrafish
Jana Oltova, Jindrich Jindrich, Ctibor Skuta, Ondrej Svoboda, Olga Machonova, Petr Bartunek
Small fish species, such as zebrafish and medaka, are increasingly gaining popularity in basic research and disease modeling as a useful alternative to rodent model organisms. However, the tracking options for fish within a facility are rather limited. In this study, we present an aquatic species tracking database, Zebrabase, developed in our zebrafish research and breeding facility that represents a practical and scalable solution and an intuitive platform for scientists, fish managers, and caretakers, in both small and large facilities...
September 20, 2018: Zebrafish
Mariana Campaner Usso, Angélica Rossotti Dos Santos, Juceli Gonzalez Gouveia, Wilson Frantine-Silva, Cristian Araya-Jaime, Maria Ligia Marques de Oliveira, Fausto Foresti, Lucia Giuliano-Caetano, Ana Lúcia Dias
Rhamdia quelen, a species of Heptapteridae, is considered to be a complex because of taxonomic and phylogenetic inconsistencies. Determining the physical location of repetitive DNA sequences on the chromosomes and the DNA barcode might increase our understanding of these inconsistencies within different groups of fish. To this end, we analyzed R. quelen populations from two river basins in Brazil, Paraguay and Parana, using DNA barcoding and different chromosomal markers, including U2 snDNA, which has never been analyzed for any Rhamdia species...
September 18, 2018: Zebrafish
Alec I M Simmonds, Clare Miln, Frank Seebacher
Sprint performance is important ecologically and physiologically, and it can influence fitness by determining outcomes of predator-prey relationships, for example, and it can confer substantial human health benefits. In this article we test whether zebrafish (Danio rerio) are a suitable model to test hypotheses about the effects and consequences of sprint exercise training, and the physiological underpinnings of sprint performance. We show that stage 3 c-starts that capture the initial escape response of fish lasting <1 s were repeatable within individuals...
September 15, 2018: Zebrafish
Víctor René Dipp, Selma Valles, Héctor Ortiz-Kerbertt, Julio V Suarez, Ulises Bardullas
Inorganic arsenic (As) is one of the most ubiquitous and toxic substances with widespread health effects on human populations and biodiversity. Although arsenic is a frequent surface water pollutant, there is scant evidence about neurotoxicity in aquatic species in different stages of development. In the present study, we investigated the neurobehavioral effects of chronic exposure to environmentally relevant doses of arsenic. We exposed zebrafish to 50 and 500 ppb during the larval, juvenile, and adult stage (from 4 h to 150 days postfertilization)...
September 5, 2018: Zebrafish
Juceli Gonzalez Gouveia, Vivian Patrícia Oliveira de Moraes-Manécolo, Ana Claudia Swarça, Alberto Sergio Fenocchio, Lucia Giuliano-Caetano, Ana Lúcia Dias
Several neotropical Siluriformes groups suffered important taxonomic revisions based on the evaluation of morphological and molecular characteristics that allow the construction of new phylogenetic hypothesis. In the present study were cytogenetically analyzed six species belonging to Heptapteridae (Cetopsorhamdia iheringi, Phenacorhamdia tenebrosa, Rhamdella eriarcha, Pimelodella meeki, Pimelodella australis, Heptapterus mustelinus) and two to Pseudopimelodidae families (Microglanis cottoides and Microglanis cibelae) by means of differential staining techniques to describe more precisely cytogenetic similarities and differences...
September 5, 2018: Zebrafish
Christopher Mark LaCoursiere, Jeremy F P Ullmann, Annapurna Poduri
Electronic databases provide effective and efficient management of zebrafish colony operations, but commercially available options are expensive. In this study we have developed a free zebrafish management repository alternative using free Google applications. Husbandry information is logged into a Google Sheets-based catalog through Google Form (GF) entries. Form autopopulation can be streamlined by barcodes, which can be generated and deciphered through free smartphone applications. The repository is capable of calculating pertinent husbandry dates from GF input and sending e-mail reminders to users for specified tasks...
September 5, 2018: Zebrafish
Melissa LaBonty, Nicholas Pray, Pamela C Yelick
Fibrodysplasia Ossificans Progressiva (FOP) is a rare, autosomal dominant genetic disorder in humans characterized by the gradual ossification of fibrous tissues, including skeletal muscle, tendons, and ligaments. In humans, mutations in the Type I BMP/TGFβ family member receptor gene, ACVR1, are associated with FOP. Zebrafish acvr1l, previously known as alk8, is the functional ortholog of human ACVR1. We previously created and characterized the first adult zebrafish model for FOP by generating animals harboring heat shock-inducible mCherry-tagged constitutively active Acvr1l (Q204D)...
September 5, 2018: Zebrafish
Seth P Johnson, Joshua D Yates, Zachary B Frederich, Jonathon T Hill
Zebrafish and other aquatic organisms depend on careful monitoring and adjustment of water quality for health and survival. This ideally includes continuous monitoring of several water parameters, including temperature, pH, conductivity, and dissolved oxygen. However, manual readings can be laborious, and commercially available monitors are cost-prohibitive for many installations, especially in small laboratories and classrooms. To address these issues, we have created ZeMo, a high-end open-source water monitoring system that includes a touchscreen, web interface, and email alerts-making continuous water quality monitoring attainable for a wide range of aquarium installations...
September 5, 2018: Zebrafish
Martina Api, Valentina Notarstefano, Ike Olivotto, Alessandro Cellerino, Oliana Carnevali
The present study was conducted to examine, for the first time in Nothobranchius furzeri, the effects of mating in different aged breeders with particular emphasis on reproductive fitness and the effects of parental aging on offspring gametogenesis. In N. furzeri, the increase of maternal and paternal age is often a predictable indicator of the upcoming deterioration on their natural habitat (i.e., ephemeral puddles) during African dry season. We previously revealed that elderly parents respond to their physiological decline state by decelerating offspring's development and growth rate...
August 28, 2018: Zebrafish
Francisco Ernani Alves Magalhães, Francisco Lucas Alves Batista, Luiza Michelly Gonçalves Lima, Izamar de Araújo Abrante, Francisca Leidivânia Alves Batista, Izabel de Araújo Abrante, José Ismael Feitosa de Araújo, Sacha Aubrey Alves Rodrigues Santos, Breytiner Amaro de Oliveira, Ramon da Silva Raposo, Adriana Rolim Campos
Zebrafish is an excellent model that can be utilized as an adjunct to current rodent models for studies of eye diseases because the anatomy and ultrastructural characterization of its cornea show much similarity with the human cornea. Therefore, we developed a behavioral model of corneal nociception using the adult zebrafish (Danio rerio). We analyzed the nociceptive effect of hypertonic saline (0.15-5.0 M sodium chloride [NaCl]) applied to the surface of the right or left cornea, on the animals' gender and locomotor activity through the open-field test...
August 28, 2018: Zebrafish
Katrina N Murray, Jeffrey C Wolf, Sean T Spagnoli, David Lains, Nadine Budrow, Michael L Kent
A laboratory zebrafish colony developed red masses, predominantly under the jaw, in a significant portion of the population. The masses were diagnosed histopathologically as thyroid follicular hyperplasia, adenoma, or carcinoma in accordance with published morphologic criteria. After switching to a higher iodine brand of salt used to maintain a low level of salinity within the water system and a small diet change, the thyroid lesions regressed dramatically. Within 5 months the masses were no longer grossly visible...
August 23, 2018: Zebrafish
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