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Zebrafish

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https://www.readbyqxmd.com/read/28933679/thyroid-hormone-stimulates-the-onset-of-adult-feeding-kinematics-in-zebrafish
#1
Sarah McMenamin, Casey Carter, Wiliam James Cooper
The physical demands for swimming and feeding change dramatically over the course of development for many aquatic animals. Indeed, in teleosts, the transition from larva to adult involves major shifts in both trophic morphology and feeding behavior. A spike in thyroid hormone (TH) coordinates many developmental processes that occur during this adult transition in numerous vertebrate species. Using mutant and transgenic zebrafish, we tested the hypothesis that TH is essential for the transition from larval to adult feeding kinematic profiles...
September 21, 2017: Zebrafish
https://www.readbyqxmd.com/read/28933662/deficiency-of-serotonin-in-raphe-neurons-and-altered-behavioral-responses-in-tryptophan-hydroxylase-2-knockout-medaka-oryzias-latipes
#2
Satoshi Ansai, Hiroshi Hosokawa, Shingo Maegawa, Kiyoshi Naruse, Youhei Washio, Kenji Sato, Masato Kinoshita
Serotonin (5-hydroxytryptamine [5-HT]) is a bioactive monoamine that acts as a neurotransmitter in the central and peripheral nervous system of animals. Teleost fish species have serotonergic neurons in the raphe nuclei of the brainstem; however, the role of 5-HT in the raphe neurons in teleost fish remains largely unknown. Here, we established a medaka (Oryzias latipes) strain with targeted disruption of tryptophan hydroxylase 2 (tph2) gene that is involved in the 5-HT synthesis in the raphe nuclei. Immunohistochemistry and mass spectrometry analysis revealed that the homozygous mutants (tph2(Δ13/Δ13)) lacked the ability to synthesize 5-HT in the raphe neurons...
September 21, 2017: Zebrafish
https://www.readbyqxmd.com/read/28910236/quantitative-responses-of-adult-zebrafish-to-changes-in-ambient-illumination
#3
Enhua Shao, Qing Bai, Yangzhong Zhou, Edward A Burton
The use of zebrafish models to study central nervous system aging and late-onset neurological diseases will be facilitated by assays allowing rapid evaluation of neurological phenotypes in adult zebrafish. We analyzed groups of 12 adult zebrafish swimming simultaneously in single-animal arenas, and quantified their responses to changes in ambient illumination. Under these conditions, stereotypical locomotor patterns were observed and readily quantified using open source software. Continuous, low-velocity movements were observed during 10-min periods of darkness, whereas intermittent high-velocity movements occurred in bright light...
September 14, 2017: Zebrafish
https://www.readbyqxmd.com/read/28880735/2017-midwest-zebrafish-meeting-report
#4
Elizabeth Sandquist, Sarah C Petersen, Cody J Smith
The 2017 Midwest Zebrafish meeting was held from June 16 to 18 at the University of Cincinnati, sponsored by the Cincinnati Children's Hospital Divisions of Developmental Biology, Molecular Cardiovascular Biology, and Gastroenterology, Hepatology, and Nutrition. The meeting, organized by Saulius Sumanas, Joshua Waxman, and Chunyue Yin, hosted >130 attendees from 16 different states. Scientific sessions were focused on morphogenesis, neural development, novel technologies, and disease models, with Steve Ekker, Stephen Potter, and Lila Solnica-Krezel presenting keynote talks...
September 7, 2017: Zebrafish
https://www.readbyqxmd.com/read/28873052/the-importance-of-holding-water-salinity-and-chemosensory-cues-affect-zebrafish-behavior
#5
Samantha Mahabir, Robert Gerlai
The zebrafish is becoming a popular model organism for studying numerous biological phenomena. Among these are brain function and behavior, including social behavior. Although usually neglected, few studies have already demonstrated that even trivial factors, such as features of the holding water may alter zebrafish behavior. In this study, we employed a 2 × 2 between-subject experimental design, exposing zebrafish to water of either high or low salinity and with chemosensory/olfactory cues of conspecifics either present or absent (while maintaining pH, temperature, nitrate, nitrite, and ammonia levels constant)...
September 5, 2017: Zebrafish
https://www.readbyqxmd.com/read/28873048/gene-ontology-based-analysis-of-zebrafish-omics-data-using-the-web-tool-comparative-gene-ontology
#6
Esmaeil Ebrahimie, Mario Fruzangohar, Seyyed Hani Moussavi Nik, Morgan Newman
Gene Ontology (GO) analysis is a powerful tool in systems biology, which uses a defined nomenclature to annotate genes/proteins within three categories: "Molecular Function," "Biological Process," and "Cellular Component." GO analysis can assist in revealing functional mechanisms underlying observed patterns in transcriptomic, genomic, and proteomic data. The already extensive and increasing use of zebrafish for modeling genetic and other diseases highlights the need to develop a GO analytical tool for this organism...
September 5, 2017: Zebrafish
https://www.readbyqxmd.com/read/28873037/breeding-zebrafish-a-review-of-different-methods-and-a-discussion-on-standardization
#7
Benjamin Tsang, Hifsa Zahid, Rida Ansari, Richard Chi-Yeung Lee, Aman Partap, Robert Gerlai
In recent years, a rapidly increasing number of scientific papers have been published that utilize zebrafish (Danio rerio) as an alternative model organism in the study of a wide range of biological phenomena from cancer to behavior. This is, in large part, due to the prolific nature, relative ease of maintenance, and sufficiently high genetic homology of zebrafish to humans. With the surge of zebrafish use in animal research, the variations in methodologies of breeding and husbandry of this species have also increased...
September 5, 2017: Zebrafish
https://www.readbyqxmd.com/read/28829283/fragile-x-mental-retardation-1-knockout-zebrafish-shows-precocious-development-in-social-behavior
#8
Yao-Ju Wu, Mao-Ting Hsu, Ming-Chong Ng, Tamara G Amstislavskaya, Maria A Tikhonova, Yi-Ling Yang, Kwok-Tung Lu
Fragile X syndrome (FXS) is a generally hereditary form of human mental retardation that is caused by triplet repeat expansion (CGG) mutation in fragile X mental retardation 1 (fmr1) gene promoter and that results in the absence of the fragile X mental retardation protein (FMRP) expression. The common symptoms of FXS patients include learning disabilities, anxiety, autistic behaviors, as well as other behavioral abnormalities. Our previous results demonstrated the behavioral abnormalities in fmr1 knockout (KO) zebrafish such as fear memory impairment and autism-like behavior...
August 22, 2017: Zebrafish
https://www.readbyqxmd.com/read/28829251/challenges-in-development-of-sperm-repositories-for-biomedical-fishes-quality-control-in-small-bodied-species
#9
Leticia Torres, Yue Liu, Amy Guitreau, Huiping Yang, Terrence R Tiersch
Quality control (QC) is essential for reproducible and efficient functioning of germplasm repositories. However, many biomedical fish models present significant QC challenges due to small body sizes (<5 cm) and miniscule sperm volumes (<5 μL). Using minimal volumes of sperm, we used Zebrafish to evaluate common QC endpoints as surrogates for fertilization success along sequential steps of cryopreservation. First, concentrations of calibration bead suspensions were evaluated with a Makler(®) counting chamber by using different sample volumes and mixing methods...
August 22, 2017: Zebrafish
https://www.readbyqxmd.com/read/28767326/tracking-of-indels-by-decomposition-is-a-simple-and-effective-method-to-assess-efficiency-of-guide-rnas-in-zebrafish
#10
Christelle Etard, Swarnima Joshi, Johannes Stegmaier, Ralf Mikut, Uwe Strähle
A bottleneck in CRISPR/Cas9 genome editing is variable efficiencies of in silico-designed gRNAs. We evaluated the sensitivity of the TIDE method (Tracking of Indels by DEcomposition) introduced by Brinkman et al. in 2014 for assessing the cutting efficiencies of gRNAs in zebrafish. We show that this simple method, which involves bulk polymerase chain reaction amplification and Sanger sequencing, is highly effective in tracking well-performing gRNAs in pools of genomic DNA derived from injected embryos. The method is equally effective for tracing INDELs in heterozygotes...
August 2, 2017: Zebrafish
https://www.readbyqxmd.com/read/28767325/evolutionary-relationships-and-cytotaxonomy-considerations-in-the-genus-pyrrhulina-characiformes-lebiasinidae
#11
Renata Luiza Rosa de Moraes, Luiz Antonio Carlos Bertollo, Manoela Maria Ferreira Marinho, Cassia Fernanda Yano, Terumi Hatanaka, Felipe Faix Barby, Waldo Pinheiro Troy, Marcelo de Bello Cioffi
Although fishes exhibit the greatest biodiversity among the vertebrates, a large percentage of this fauna is still underexplored on evolutionary cytogenetic questions, particularly the miniature species. The Lebiasinidae family is a particular example for such case. This study is the first one presenting differential cytogenetic methods, such as C-banding, repetitive DNAs mapping, comparative genomic hybridization (CGH), and whole chromosome painting in lebiasinid species. Pyrrhulina australis and Pyrrhulina aff...
August 2, 2017: Zebrafish
https://www.readbyqxmd.com/read/28737485/risk-taking-during-feeding-between-and-within-population-variation-and-repeatability-across-contexts-among-wild-zebrafish
#12
Tamal Roy, Rohitashva Shukla, Anuradha Bhat
Behavioral responses are shaped by a complex interplay of extrinsic factors, such as predation and flow conditions, and intrinsic properties such as metabolic state. We investigated variations in boldness among four populations of zebrafish collected from widely varying habitat conditions across India. We measured feeding latencies of fish in four contexts: in a novel environment; in an environment with refuge and predators; in the presence of a shoal; and in the presence of a shoal and predators. The population from a high-flow and high-predation habitat was significantly bolder than populations from low-predation and low-flow/stagnant habitats...
July 24, 2017: Zebrafish
https://www.readbyqxmd.com/read/28727940/intestinal-transit-time-and-cortisol-mediated-stress-in-zebrafish
#13
Clayton Brady, Maxwell Denora, Ian Shannon, Karl J Clark, Adam Rich
Intestinal motility, the spontaneous and rhythmic smooth muscle contraction, is a complex process that is regulated by overlapping and redundant regulatory mechanisms. Primary regulators intrinsic to the gastrointestinal tract include interstitial cells of Cajal, enteric neurons, and smooth muscle cells. Extrinsic primary regulators include the autonomic nervous system, immune system, and the endocrine system. Due to this complexity, a reductionist approach may be inappropriate if the ultimate goal is to understand motility regulation in vivo...
July 20, 2017: Zebrafish
https://www.readbyqxmd.com/read/28714785/effects-of-alcohol-on-inhibitory-avoidance-learning-in-zebrafish-danio-rerio
#14
Ricardo Rodrigues Amorim, Priscila Fernandes Silva, Ana Carolina Luchiari
The zebrafish (Danio rerio) can be used in studies addressing the effects of drugs on learning, memory, and anxiety. In the present study, we investigated the effect of different alcohol treatments (chronic and acute) on the learning and anxiety response of zebrafish in an inhibitory avoidance paradigm. Zebrafish were initially exposed to different alcohol treatments and submitted to an inhibitory avoidance protocol, where an electroshock was applied to the fish as they swam from the white to the black side of a shuttle box tank (naturally preferred environment of zebrafish)...
July 17, 2017: Zebrafish
https://www.readbyqxmd.com/read/28704145/adult-zebrafish-danio-rerio-an-alternative-behavioral-model-of-formalin-induced-nociception
#15
Francisco Ernani Alves Magalhães, Caio Átila Prata Bezerra de Sousa, Sacha Aubrey Alves Rodrigues Santos, Renata Barbosa Menezes, Francisco Lucas Alves Batista, Ângela Oliveira Abreu, Messias Vital de Oliveira, Luiz Francisco Wemmenson Gonçalves Moura, Ramon da Silva Raposo, Adriana Rolim Campos
The zebrafish (Danio rerio) has been proposed as a low-cost and simple alternative to the use of higher vertebrates in laboratory research on novel compounds with antinociceptive potential. In this study, we tested adult zebrafish (Danio rerio) as an alternative behavioral model of formalin-induced nociception. We evaluated the nociceptive effect of 0.1% formalin (3 or 5 μL; intramuscularly [i.m.]), applied into the tail or lips, on locomotor activity, using as parameter the number of times the fish crossed the lines between the quadrants of a glass Petri dish during the neurogenic stage (0-5 min) and the inflammatory stage (15-30 min)...
July 13, 2017: Zebrafish
https://www.readbyqxmd.com/read/28678656/a-simple-and-inexpensive-microinjection-system-for-zebrafish-embryos-and-larvae
#16
Seyed-Mohammadreza Samaee, Hanieh Nikkhah, Zoltan M Varga, Bahman Rezaei
Microinjection is a widely used technique to inject defined volumes and concentrations of substances and explore their physiological function in vivo. The technique has been particularly successful with zebrafish embryos; however, the injection equipment can be relatively expensive and therefore available only to well-funded laboratories. In this study, a simple, cheap, easy-to-assemble, and easy-to-use setup with a straightforward, accurate, and efficacious calibration method is introduced. The accuracy of this calibration method was tested by comparing with the results of calibration methods that are currently used in high-cost systems...
July 5, 2017: Zebrafish
https://www.readbyqxmd.com/read/28678643/a-novel-photocatalytic-purification-system-for-fish-culture
#17
Basilio Randazzo, Giulia Chemello, Ivan Tortarolo, Gian Luca Chiarello, Maciej Zalas, Alessandra Santini, Margaret Liberatore, Marco Liberatore, Elena Selli, Ike Olivotto
The accumulation of nitrogen compounds represents a pivotal problem in the management of fish culture. Several methods were investigated in the last decades for treatment of waste waters, and the use of photocatalytic materials has received increasing attention. The photocatalytic degradation (PCD) process with titanium dioxide (TiO2) represents the most promising single-step method to promote the removal of nitrogen compounds from water. The present study compares for the first time the effects on fish culture of a classical mechanical, biological, and ultraviolet purification system to a TiO2-PCD one, with particular emphasis on water chemistry and on physiological responses in zebrafish...
July 5, 2017: Zebrafish
https://www.readbyqxmd.com/read/28609254/chromosomal-diversity-of-three-species-of-hypostomus-lac%C3%A3-p%C3%A3-de-1803-siluriformes-loricariidae-from-the-paran%C3%A3-river-basin-brazil-a-species-complex-in-hypostomus-ancistroides-reinforced-by-a-zz-zw-sex-chromosome-system
#18
Marcia Cristina de Souza Lara Kamei, Lucas Baumgärtner, Suzana Paiva, Cláudio Henrique Zawadzki, Isabel Cristina Martins-Santos, Ana Luiza de Brito Portela-Castro
Hypostomus shows wide morphological patterns, corroborated by great chromosomal diversity that has suggested the existence of new species, especially from small tributaries. Cytogenetic analysis has contributed to estimate a cryptic diversity providing important data for taxonomic and evolutionary studies. Cytogenetic techniques were carried out on species from a small tributary of Ivaí River, Keller River (upper Paraná River basin): Hypostomus aff. ancistroides, Hypostomus topavae, and Hypostomus aff. hermanni that presented 2n = 68, 80, and 72 chromosomes, respectively...
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28570143/zebrafish-and-the-cancer-moonshot
#19
Lisa A Schimmenti
No abstract text is available yet for this article.
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28557653/efficient-transduction-of-zebrafish-melanoma-cell-lines-and-embryos-using-lentiviral-vectors
#20
Maurizio Fazio, Serine Avagyan, Ellen van Rooijen, William Mannherz, Charles K Kaufman, Riadh Lobbardi, David M Langenau, Leonard I Zon
The establishment of in vitro cultures of zebrafish cancer cells has expanded the potential of zebrafish as a disease model. However, the lack of effective methods for gene delivery and genetic manipulation has limited the experimental applications of these cultures. To overcome this barrier, we tested and optimized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviral and retroviral vector transduction protocols. We show that lentivirus successfully and efficiently transduced zebrafish melanoma cell lines in vitro, allowing antibiotic selection, fluorescence-based sorting, and in vivo allotransplantation...
August 2017: Zebrafish
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