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Víctor René Dipp, Selma Valles, Héctor Ortiz-Kerbertt, Julio V Suarez, Ulises Bardullas
Inorganic arsenic (As) is one of the most ubiquitous and toxic substances with widespread health effects on human populations and biodiversity. Although arsenic is a frequent surface water pollutant, there is scant evidence about neurotoxicity in aquatic species in different stages of development. In the present study, we investigated the neurobehavioral effects of chronic exposure to environmentally relevant doses of arsenic. We exposed zebrafish to 50 and 500 ppb during the larval, juvenile, and adult stage (from 4 h to 150 days postfertilization)...
September 5, 2018: Zebrafish
Juceli Gonzalez Gouveia, Vivian Patrícia Oliveira de Moraes-Manécolo, Ana Claudia Swarça, Alberto Sergio Fenocchio, Lucia Giuliano-Caetano, Ana Lúcia Dias
Several neotropical Siluriformes groups suffered important taxonomic revisions based on the evaluation of morphological and molecular characteristics that allow the construction of new phylogenetic hypothesis. In the present study were cytogenetically analyzed six species belonging to Heptapteridae (Cetopsorhamdia iheringi, Phenacorhamdia tenebrosa, Rhamdella eriarcha, Pimelodella meeki, Pimelodella australis, Heptapterus mustelinus) and two to Pseudopimelodidae families (Microglanis cottoides and Microglanis cibelae) by means of differential staining techniques to describe more precisely cytogenetic similarities and differences...
September 5, 2018: Zebrafish
Christopher Mark LaCoursiere, Jeremy F P Ullmann, Annapurna Poduri
Electronic databases provide effective and efficient management of zebrafish colony operations, but commercially available options are expensive. In this study we have developed a free zebrafish management repository alternative using free Google applications. Husbandry information is logged into a Google Sheets-based catalog through Google Form (GF) entries. Form autopopulation can be streamlined by barcodes, which can be generated and deciphered through free smartphone applications. The repository is capable of calculating pertinent husbandry dates from GF input and sending e-mail reminders to users for specified tasks...
September 5, 2018: Zebrafish
Melissa LaBonty, Nicholas Pray, Pamela C Yelick
Fibrodysplasia Ossificans Progressiva (FOP) is a rare, autosomal dominant genetic disorder in humans characterized by the gradual ossification of fibrous tissues, including skeletal muscle, tendons, and ligaments. In humans, mutations in the Type I BMP/TGFβ family member receptor gene, ACVR1, are associated with FOP. Zebrafish acvr1l, previously known as alk8, is the functional ortholog of human ACVR1. We previously created and characterized the first adult zebrafish model for FOP by generating animals harboring heat shock-inducible mCherry-tagged constitutively active Acvr1l (Q204D)...
September 5, 2018: Zebrafish
Seth P Johnson, Joshua D Yates, Zachary B Frederich, Jonathon T Hill
Zebrafish and other aquatic organisms depend on careful monitoring and adjustment of water quality for health and survival. This ideally includes continuous monitoring of several water parameters, including temperature, pH, conductivity, and dissolved oxygen. However, manual readings can be laborious, and commercially available monitors are cost-prohibitive for many installations, especially in small laboratories and classrooms. To address these issues, we have created ZeMo, a high-end open-source water monitoring system that includes a touchscreen, web interface, and email alerts-making continuous water quality monitoring attainable for a wide range of aquarium installations...
September 5, 2018: Zebrafish
Martina Api, Valentina Notarstefano, Ike Olivotto, Alessandro Cellerino, Oliana Carnevali
The present study was conducted to examine, for the first time in Nothobranchius furzeri, the effects of mating in different aged breeders with particular emphasis on reproductive fitness and the effects of parental aging on offspring gametogenesis. In N. furzeri, the increase of maternal and paternal age is often a predictable indicator of the upcoming deterioration on their natural habitat (i.e., ephemeral puddles) during African dry season. We previously revealed that elderly parents respond to their physiological decline state by decelerating offspring's development and growth rate...
August 28, 2018: Zebrafish
Francisco Ernani Alves Magalhães, Francisco Lucas Alves Batista, Luiza Michelly Gonçalves Lima, Izamar de Araújo Abrante, Francisca Leidivânia Alves Batista, Izabel de Araújo Abrante, José Ismael Feitosa de Araújo, Sacha Aubrey Alves Rodrigues Santos, Breytiner Amaro de Oliveira, Ramon da Silva Raposo, Adriana Rolim Campos
Zebrafish is an excellent model that can be utilized as an adjunct to current rodent models for studies of eye diseases because the anatomy and ultrastructural characterization of its cornea show much similarity with the human cornea. Therefore, we developed a behavioral model of corneal nociception using the adult zebrafish (Danio rerio). We analyzed the nociceptive effect of hypertonic saline (0.15-5.0 M sodium chloride [NaCl]) applied to the surface of the right or left cornea, on the animals' gender and locomotor activity through the open-field test...
August 28, 2018: Zebrafish
Molly R Morris, Ryan J Friebertshauser, Megan Zupi, Melissa N Liotta, Garrett Dunn, Nicole Kleinas, Oscar Rios-Cardenas
A better understanding of the role of appetite regulation in obesity and metabolic disorders requires consideration of both genetic and developmental influences on appetite. Previously we detected genetic differences in responses to nutritional programming (e.g., the permanent influence that nutrition in early life has on the physiological and metabolic states in adults) on a presumed measure of appetite (feeding rate) in the swordtail fish Xiphophorus multilineatus. In this study we validate that feeding rate is a good measure of appetite, by first demonstrating that it is repeatable and correlated with food consumed when controlling for body size...
August 23, 2018: Zebrafish
Katrina N Murray, Jeffrey C Wolf, Sean T Spagnoli, David Lains, Nadine Budrow, Michael L Kent
A laboratory zebrafish colony developed red masses, predominantly under the jaw, in a significant portion of the population. The masses were diagnosed histopathologically as thyroid follicular hyperplasia, adenoma, or carcinoma in accordance with published morphologic criteria. After switching to a higher iodine brand of salt used to maintain a low level of salinity within the water system and a small diet change, the thyroid lesions regressed dramatically. Within 5 months the masses were no longer grossly visible...
August 23, 2018: Zebrafish
Andrey D Volgin, Oleg V Yakovlev, Konstantin A Demin, Murilo S de Abreu, Denis B Rosemberg, Darya A Meshalkina, Polina A Alekseeva, Ashton J Friend, Tamara G Amstislavskaya, Allan V Kalueff
Environmental stimuli are critical in preclinical research that utilizes laboratory animals to model human brain disorders. The main goal of environmental enrichment (EE) is to provide laboratory animals with better choice of activity and greater control over social and spatial stressors. Thus, in addition to being a useful experimental tool, EE becomes an important strategy for increasing the validity and reproducibility of preclinical data. Although zebrafish (Danio rerio) is rapidly becoming a promising new organism for neuroscience research, the role of EE in zebrafish central nervous system (CNS) models remains poorly understood...
August 22, 2018: Zebrafish
Hanseul Oh, C-Yoon Kim, Bokyeong Ryu, Ukjin Kim, Jin Kim, Ji-Min Lee, Byoung-Hee Lee, Jisook Moon, Cho-Rok Jung, Jae-Hak Park
Humidifier disinfectants containing polyhexamethylene guanidine phosphate (PHMG-P) can induce pulmonary toxicity and has caused human casualties in South Korea since 2006. Thereby, the safety evaluation of household chemicals such as PHMG-P has garnered increased importance. However, many limitations, such as the lack of specialized facilities and animal welfare concerns associated with the use of murine models, persist. Zebrafish gills have high functional and structural similarity to mammalian lungs. Moreover, zebrafish are sensitive to toxic substances, resulting in changes in behavioral or ventilatory patterns...
August 22, 2018: Zebrafish
Kavinash Loganathan, Shogo Moriya, Ishwar S Parhar
Ambient light and temperature affect reproductive function by regulating kisspeptin and gonadotrophin-releasing hormone (GnRH) in vertebrates. Melatonin and melatonin receptors, as well as the two-pore domain K+ channel-related K+ (TREK) channels, are affected by light and/or temperature; therefore, these molecules could modulate kisspeptin and GnRH against ambient light and temperature. In this study, we investigated the effect of light and temperature, which affect melatonin levels in gene expression levels of TREK channels, kisspeptin, and GnRH...
August 13, 2018: Zebrafish
Amy Kolb, Friedhelm Hildebrandt, Christian Lawrence
Zebrafish (Danio rerio) have proven their efficiency as an animal model for genetics and development, but their nutrition and housing requirements continue to elude researchers. Diet and housing density were predicted to affect weight change and reproductive success in 120 days postfertilization (dpf) zebrafish, and growth performance of their progeny. Fish were fed one of four diets, each utilizing a different primary protein source (fish meal [Zeigler™], algae, or insect), while being housed 3.3 or 6.6 fish/L for 3 weeks...
August 13, 2018: Zebrafish
Rafael E Hernandez, Louie Galitan, James Cameron, Nicola Goodwin, Lalita Ramakrishnan
The use of early-stage zebrafish for biomedical research spans early organogenesis to free-swimming larva. A key benefit of this model organism is that repeated assessments spanning several days can be performed of individual larvae within a single experiment, often in conjunction with administered drugs. However, the initiation of feeding, typically at 5 days postfertilization (dpf), can make serial assessments challenging. Therefore, delayed feeding would increase the utility of the model. To ask whether feeding could be delayed without adversely affecting larval growth and development up to 39 dpf, we systematically raised zebrafish and introduced feeding at 5 dpf or delayed initial feeding up to 9 dpf...
August 8, 2018: Zebrafish
Tommaso Ruberto, Romain J G Clément, Chiara Spinello, Daniele Neri, Simone Macrì, Maurizio Porfiri
One commonly used method to preserve individual identity in the study of social behavior of zebrafish is through silicone-based visible implant elastomers (VIEs), which represent a safe and durable tagging procedure. While the effects of VIE tagging on welfare and general health have been addressed in detail, whether this procedure influences social behavior remains unclear. In this study, we compared individual and group behaviors exhibited by shoals composed of three individuals: two nontagged and one (focal subject) that was either nontagged (control condition) or sham-, purple-, blue-, or yellow tagged...
August 2, 2018: Zebrafish
Ana Camila Prizon, Daniel Pacheco Bruschi, Camilla Borges Gazolla, Luciana Andrea Borin-Carvalho, Ana Luiza de Brito Portela-Castro
The repetitive DNAs are the expressive substrate to genomic evolution and directly related to chromosomal diversification in eukaryote, including fishes. Ancistrus is an interesting group for studies about interplay between repetitive DNA and karyotype evolution, given its extensive chromosomal variation. In this study, we aimed to understand the evolutionary dynamics in genome of the distinct Ancistrus populations of the Paraná basin to the contribution of three classes of repetitive DNA sequences. Nucleotide sequence was isolated, characterized the nonlong terminal repeat (non-LTR) retrotransposable Rex-3, and evaluated the chromosomal organization in the Ancistrus populations...
July 26, 2018: Zebrafish
Mauricio da Silva Sopezki, José Maria Monserrat, João Sarkis Yunes, Juliano Zanette
Microcystin-leucine arginine (MC-LR) is a natural toxin produced by cyanobacterial blooms that causes severe liver damage in fish. It is crucial to investigate if housekeeping genes are affected by MC-LR in zebrafish, to permit the adequate evaluation of gene expression by RT-qPCR. We evaluated the gene expression stability (M value) and regulation by chemical treatment (using E-Ct and E-ΔCt ) to validate the use of eight housekeeping genes in fish exposed to 0, 0.31, and 6.10 μg L-1 MC-LR for 24 h. We suggest the use of the combination of β-actin1, b2m, and arnt2 in the liver and β-actin1, 18S rRNA, and arnt2 in gills as housekeeping genes...
July 25, 2018: Zebrafish
Vanessa Bueno, Jocicléia Thums Konerat, Cláudio Henrique Zawadzki, Paulo Cesar Venere, Daniel Rodrigues Blanco, Vladimir Pavan Margarido
Hypostominae is the largest subfamily of Loricariidae, and is widely distributed throughout the Neotropic. In the present article, we analyze three Loricariidae species that were considered part of Hypostominae, from three different tribes, to discuss chromosome evolution in this fish group and to review the existent data for the subfamily. Rhinelepis aspera had 54 chromosomes (20m + 26sm+8st), whereas Pterygoplichthys ambrosettii and Megalancistrus parananus had 52 chromosomes, with 16m+24sm+8st+4a and 18m+24sm+10st, respectively...
June 29, 2018: Zebrafish
Julia Tandberg, Leidy Lagos, Erik Ropstad, Gro Smistad, Marianne Hiorth, Hanne C Winther-Larsen
The introduction of fish vaccination has had a tremendous impact on the aquaculture industry by providing an important measurement in regard to disease control. Infectious diseases caused by intracellular pathogens do, however, remain an unsolved problem for the industry. This is in many cases directly connected to the inability of vaccines to evoke a cellular immunity needed for long-term protection. Thus, there is a need for new and improved vaccines and adjuvants able to induce a strong humoral and cellular immune response...
August 2018: Zebrafish
Ricardo Utsunomia, Silvana Melo, Priscilla Cardim Scacchetti, Claudio Oliveira, Milla de Andrade Machado, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi, Fausto Foresti
Microsatellites show great abundance in eukaryotic genomes, although distinct chromosomal distribution patterns might be observed, from small dispersed signals to strong clustered motifs. In Neotropical fishes, the chromosome mapping of distinct microsatellites was employed several times to uncover the origin and evolution of sex and supernumerary chromosomes, whereas a detailed comparative analysis considering different motifs at the chromosomal level is scarce. Here, we report the chromosomal location of several simple sequence repeats (SSRs) in distinct electric knife fishes showing variable diploid chromosome numbers to unveil the structural organization of several microsatellite motifs in distinct Gymnotus species...
August 2018: Zebrafish
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