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Konrad Dabrowski, Mackenzie Miller
The current body of work on rearing larval/juvenile zebrafish is based on (1) utilization of freshwater and (2) diurnal light/dark cycle, (3) provision of live feed at modest density, and (4) culture in high visibility environment. We challenged these rearing approaches by maintaining zebrafish under constant light for 46-48 days (days postfertilization [dpf]), while securing continuous feeding in high turbidity and saline (1.8-2.1 parts per thousand) environment for the experiment's duration, allowing 24 h feeding/growth of fish from first exogenous feeding to maturation...
February 27, 2018: Zebrafish
Sebastien Andrew Rider, Finnius Austin Bruton, Richard George Collins, Bryan Ronald Conway, John James Mullins
Defects in the glomerular filtration barrier (GFB) play a major role in the onset of human renal diseases. Highly ramified glomerular cells named podocytes are a critical component of the GFB. Injury to podocytes results in abnormal excretion of plasma proteins, which can lead to chronic kidney disease. The conserved paired nephron of larval zebrafish is an excellent model for assessing glomerular function and injury. The efficacy of two known podocyte toxins was tested to refine models of acute podocyte injury in larval zebrafish...
February 26, 2018: Zebrafish
Tyrone J Tolbert, Shinnosuke Nakayama, Maurizio Porfiri
The advent of automated tracking software has significantly reduced the time required to record movement trajectories, thereby facilitating behavioral studies of zebrafish. However, results are substantially influenced by tracking errors, such as loss and misidentification of individuals. In this study, we present the development of an online citizen science platform, Tracking Nemo, to improve data accuracy on swimming trajectories of zebrafish groups. As an online extension of software for tracking the position of zebrafish from video recordings, Tracking Nemo offers volunteers the opportunity to contribute to science by manually correcting tracked trajectory data from their personal computers...
February 22, 2018: Zebrafish
Heather M Prior, Kerri Letwin, Amber Tuininga, Michelle Nguyen
We have developed a simple method for the induction of cataracts in adult zebrafish using hydrogen peroxide. Our method uses a corneal puncture followed by intraocular injection of hydrogen peroxide into the anterior chamber. The procedure can be done rapidly, forming a highly opaque cataract almost immediately, and fish recover quickly. The optical clarity of the lens is gradually regained over time, showing the innate ability of zebrafish to regenerate healthy lens tissue. This technique provides a simple in vivo system with which to assess mutant strains or targeted disruptions to genes expressed in the lens...
February 13, 2018: Zebrafish
Ananda Marques Pety, Adauto Lima Cardoso, Cleusa Yoshiko Nagamachi, Julio Cesar Pieczarka, Leandro Melo de Sousa, Renata Coelho Rodrigues Noronha
Loricariidae is a diverse group of fish from the neotropical region, occupying a wide variety of freshwater environments. Cytogenetic data have brought important insights into Loricariidae diversity because they help validate undescribed species as well as our understanding of inter- and intraspecific diversity. However, conventional cytogenetic approaches are limited in their ability to detect variability in some lineages, as seen in the Peckoltia clade, owing to their apparent conserved karyotype. Thus, the aim of this work was to map 5S and 18S ribosomal (rDNA) sites in five species of Peckoltia and one species of Ancistomus from the Amazon basin, and discusses the mechanisms of organization and diversification of these clusters...
February 8, 2018: Zebrafish
Rohit C Philip, Jeffrey J Rodriguez, Maki Niihori, Ross H Francis, Jordan A Mudery, Justin S Caskey, Elizabeth Krupinski, Abraham Jacob
Zebrafish have emerged as a powerful biological system for drug development against hearing loss. Zebrafish hair cells, contained within neuromasts along the lateral line, can be damaged with exposure to ototoxins, and therefore, pre-exposure to potentially otoprotective compounds can be a means of identifying promising new drug candidates. Unfortunately, anatomical assays of hair cell damage are typically low-throughput and labor intensive, requiring trained experts to manually score hair cell damage in fluorescence or confocal images...
January 30, 2018: Zebrafish
Melissa L Fangmeier, Daniel W A Noble, Rose E O'Dea, Takuji Usui, Malgorzata Lagisz, Daniel Hesselson, Shinichi Nakagawa
An emergent field of animal personality necessitates a method for repeated high-throughput quantification of behavioral traits across contexts. In this study, we have developed an automated video stimulus approach to sequentially present different contexts relevant to five "personality" traits (exploration, boldness, neophobia, aggression, and sociability), successfully quantifying repeatable trait measurements in multiple individuals simultaneously. Although our method is designed to quantify personality traits in zebrafish, our approach can accommodate the quantification of other behaviors, and could be customized for other species...
January 30, 2018: Zebrafish
Zu Soh, Motoki Matsuno, Masayuki Yoshida, Toshio Tsuji
Fear and anxiety in fish are generally evaluated by video-based behavioral analysis. However, it is difficult to distinguish the psychological state of fish exclusively through video analysis, particularly whether the fish are freezing, which represents typical fear behavior, or merely resting. We propose a system that can measure bioelectrical signals called ventilatory signals and simultaneously analyze swimming behavior in real time. Experimental results comparing the behavioral analysis of the proposed system and the camera system showed a low error level with an average absolute position error of 9...
January 30, 2018: Zebrafish
Ana Beatriz Delavia Thomasi, Carmen Sonntag, Danilo Fernando Pires, David Zuidema, Antonio Benci, Peter David Currie, Alasdair John Wood
Muscle fiber detachment from myoseptal boundaries is a common finding in zebrafish models of muscular dystrophies. In some instances, there is a weakening of the interaction between muscle fiber and myosepta, which is yet to manifest as a fiber detachment phenotype. Therefore, to push the fiber detachment of muscle, mutant fish but not their wild-type siblings, beyond their binding threshold, a series of small electrical pulses can be applied to the larvae to create a maximal force contraction and ultimately fiber detachment...
January 30, 2018: Zebrafish
Murilo S Abreu, Thiago A Oliveira, Gessi Koakoski, Rodrigo E Barreto, Leonardo J G Barcellos
Studies regarding predator-prey relationships have often focused on prey assessment and the responses to predation risk, but few have verified the relationship in the presence or absence of visual contact with a predator (e.g., tiger oscar, Astronotus ocellatus) or a nonpredator (e.g., goldfish, Carassius auratus) during the developmental phase, which could alter several physiological and neuroendocrine mechanisms in adulthood. Herein, we determined responses to physical (chasing with a net) and biological stressors (visualization to predator) in adult zebrafish raised in visual contact with a predator or nonpredator fish...
January 25, 2018: Zebrafish
Lauren J Norris, Virginia Watral, Michael L Kent
Cryopreservation is a common method used to preserve the sperm of various animal species, and it is widely used with zebrafish (Danio rerio). As with other animals, there is a possibility of paternal pathogen transmission through sperm. We evaluated the ability of five common and important pathogens of zebrafish to survive cryopreservation as used with zebrafish sperm and freezing without cryopreservant. We evaluated Mycobacterium chelonae, Mycobacterium marinum, and Edwardsiella ictaluri, each originally isolated from zebrafish, eggs of Pseuodocapillaria tomentosa, and spores of Pseudoloma neurophilia...
January 25, 2018: Zebrafish
Clay Patton, Gist H Farr, Ding An, Paolo G V Martini, Lisa Maves
Lipid nanoparticles (LNPs) are an attractive platform for the delivery of therapeutic RNA molecules because LNPs are versatile, have been validated in clinical trials, and are well tolerated. Here, we test whether LNPs can be used to deliver a reporter green fluorescent protein (gfp) mRNA to different tissues in zebrafish embryos. We show that LNP-packaged gfp mRNA can be delivered, through injection, and taken up by cells in multiple tissues in zebrafish embryos without any apparent detrimental effects on embryonic health or survival...
January 25, 2018: Zebrafish
Jennifer L Matthews, Joy M Murphy, Carrie Carmichael, Huiping Yang, Terrence Tiersch, Monte Westerfield, Zoltan M Varga
Sperm cryopreservation is a highly efficient method for preserving genetic resources. It extends the reproductive period of males and significantly reduces costs normally associated with maintenance of live animal colonies. However, previous zebrafish (Danio rerio) cryopreservation methods have produced variable outcomes and low post-thaw fertilization rates. To improve post-thaw fertilization rates after cryopreservation, we developed a new extender and cryoprotective medium (CPM), introduced quality assessment (QA), determined the optimal cooling rate, and improved the post-thaw in vitro fertilization process...
January 25, 2018: Zebrafish
Rebecca Wilk, Naomi Ali, Samantha J England, Katharine E Lewis
Zebrafish are widely used as a model organism for research. Zebrafish embryos are also a useful resource for teaching students about vertebrate development. Here we describe a collaboration between two high school teachers and two university professors that used zebrafish to bring hands-on laboratory experiences to inner-city students, with the aim of increasing tangibility, and improving student understanding and retention, of several fundamental scientific concepts, such as the scientific method, cell division, mitosis, and Mendelian genetics...
January 22, 2018: Zebrafish
Máté Varga
The history of science offers multiple examples of how the perseverance of a single visionary person could open the floodgates for a whole new area of research. Zebrafish research is one of these fields with an exciting founding story, as it was the dogged persistence of one man, George Streisinger, that ultimately lifted this little fish out of the obscurity of pet shops into the pantheon of genetic model organisms. The Hungarian born Streisinger was one of the most gifted geneticists of his era and his network of mentors and friends really reads like a who-is-who of 20th century genetics...
January 5, 2018: Zebrafish
Simone Rutz Costa, Robson Rabelo Velasques, Monique Tomazele Rovani, Marta Marques Souza, Juliana Zomer Sandrini
Fish cellular models are commonly used to study the toxic potential of environmentally relevant compounds. Several of these pollutants act on DNA and compromise its integrity. Little is known, however, about the DNA repair ability of these cellular models. Therefore, the aim of this study was to evaluate the DNA base excision repair (BER) of zebrafish Liver (ZF-L) cell line and primary hepatocytes. We performed kinetic studies of the DNA damage levels after exposure to hydrogen peroxide (H2O2, 20 μM for 10 min) using the Comet Assay...
January 5, 2018: Zebrafish
Martina Api, Piera Biondi, Ike Olivotto, Eva Terzibasi, Alessandro Cellerino, Oliana Carnevali
Studies on parental aging are a very attractive field, although it is poorly understood how parental age affects embryonic development and adult traits of the offspring. In this study, we used the turquoise killifish Nothobranchius furzeri, as is the vertebrate with shortest captive lifespan and an interesting model. The embryos of N. furzeri can follow two distinct developmental pathways either entering diapause or proceeding through direct development. Thus, this embryonic plasticity allows this model to be used to study different factors that could affect their embryonic development, including parental age...
January 5, 2018: Zebrafish
Jorge Guerra-Varela, Maite Baz-Martínez, Sabela Da Silva-Álvarez, Ana Paula Losada, María Isabel Quiroga, Manuel Collado, Carmen Rivas, Laura Sánchez
The zebrafish, Danio rerio, has become recognized as a valuable model for infectious diseases. Here we evaluated the susceptibility of zebrafish to be infected with the mammalian vesicular stomatitis virus (VSV). Both zebrafish cells and embryos were highly susceptible to VSV infection. Mortalities exceeded 80% in infected embryos and were preceded by the invasion of the central nervous system by VSV. Live imaging of the infection with GFP-VSV as well as virus titration from infected fish confirmed the viral replication...
January 5, 2018: Zebrafish
Marc T Tye, Jacob E Montgomery, Maurine R Hobbs, Kayce T Vanpelt, Mark A Masino
The lack of standardized diet for laboratory animals can have profound effects on animal health and lead to less reproducible research outcomes. Live diets are commonly used in zebrafish culture and, although they are a more natural feed than flake or pellet food, are also a potential source of pathogens and toxic compounds. Heavy metals are a group of such compounds, which can accumulate in fish leading to developmental abnormalities, reduced growth, and increased rates of mortality. Two to three weeks after feeding adult zebrafish a new lot of nonhatching decapsulated brine shrimp cysts (Decaps), embryos at the University of Minnesota Zebrafish Core Facility (ZCF) and the University of Utah Centralized Zebrafish Animal Resource (CZAR) began to exhibit an orange color in the yolk, and larval health began to decline...
January 2, 2018: Zebrafish
Jana Oltová, Carrie Barton, Ana Catarina Certal, Francesco Argenton, Zoltán M Varga
A husbandry workshop on July 3, 2017, at the 10th European Zebrafish Meeting in Budapest, Hungary (July 3-July 7, 2017), focused on the standardization, optimization, and streamlining of fish facility procedures. Standardization can be achieved for example by developing novel software and hardware tools, such as a fish facility database for husbandry and environmental facility management (Zebrabase, Oltova), or a hand-held, air-pressurized fish feeder for consistent food distribution (Blowfish, Argenton). Streamlining is achieved when work hours are reduced, as with the standardized fish feeder, or by limiting the number and types of fish diets and observing the effect on animal welfare and performance (Barton)...
January 2, 2018: Zebrafish
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