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Zebrafish

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https://www.readbyqxmd.com/read/28520533/a-fully-automated-high-throughput-zebrafish-behavioral-ototoxicity-assay
#1
Douglas W Todd, Rohit C Philip, Maki Niihori, Ryan A Ringle, Kelsey R Coyle, Sobia F Zehri, Jordan A Mudery, Ross H Francis, Jeffrey J Rodriguez, Abraham Jacob
Zebrafish animal models lend themselves to behavioral assays that can facilitate rapid screening of ototoxic, otoprotective, and otoregenerative drugs. Structurally similar to human inner ear hair cells, the mechanosensory hair cells on their lateral line allow the zebrafish to sense water flow and orient head-to-current in a behavior called rheotaxis. This rheotaxis behavior deteriorates in a dose-dependent manner with increased exposure to the ototoxin cisplatin, thereby establishing itself as an excellent biomarker for anatomic damage to lateral line hair cells...
May 18, 2017: Zebrafish
https://www.readbyqxmd.com/read/28488936/long-term-social-recognition-memory-in-zebrafish
#2
Natália Madeira, Rui F Oliveira
In species in which individuals live in stable social groups, individual recognition is expected to evolve to allow individuals to remember past interactions with different individuals and adjust future behavior toward them accordingly. Thus, social memory is expected to be a ubiquitous component of social cognition of social species. However, few studies have investigated the occurrence of social memory in non-mammals. Here we evaluated the ability of zebrafish (Danio rerio) to recognize different conspecifics and to retain this information in long lasting (i...
May 10, 2017: Zebrafish
https://www.readbyqxmd.com/read/28488934/the-zebrafish-curly-fry-is-required-for-proper-centrosome-and-mitotic-spindle-assembly
#3
Mi Hye Song, Jeffrey C Medley, John Y Kuwada
The zebrafish curly fry (cfy) mutation leads to a dramatic increase in mitotic index and cell death starting during neural tube formation. The mutant phenotype is cell autonomous and does not result from defects in apical/basal polarity within the neuroepithelium. The increase in mitotic index could be due to increased proliferation or cell cycle arrest in mitosis. cfy embryos were analyzed to examine these two possibilities. By labeling embryos with a pulse of BrdU and anti-phospho-histone 3 and examining the DNA content by fluorescence activated cell sorting, we show that cfy mutants exhibit no increase in proliferation, but a significant increase in the number of cells arrested in mitosis...
May 10, 2017: Zebrafish
https://www.readbyqxmd.com/read/28488933/zebrafish-as-a-screening-model-for-testing-the-permeability-of-blood-brain-barrier-to-small-molecules
#4
Seong Soon Kim, So Hee Im, Jung Yoon Yang, Yu-Ri Lee, Geum Ran Kim, Jin Sil Chae, Dae-Seop Shin, Jin Sook Song, Sunjoo Ahn, Byung Hoi Lee, Jae Chun Woo, Jin Hee Ahn, Chang Soo Yun, Phiho Kim, Hyoung Rae Kim, Kyeong-Ryoon Lee, Myung Ae Bae
The objective of this study was to evaluate the permeability of small molecules into the brain via the blood-brain barrier in zebrafish and to investigate the possibility of using this animal model as a screening tool during the early stages of drug discovery. Fifteen compounds were used to understand the permeation into the brain in zebrafish and mice. The ratio of brain-to-plasma concentration was compared between the two animal models. The partition coefficient (Kp,brain), estimated using the concentration ratio at designated times (0...
May 10, 2017: Zebrafish
https://www.readbyqxmd.com/read/28459655/comparative-analyses-of-zebrafish-anxiety-like-behavior-using-conflict-based-novelty-tests
#5
Elana V Kysil, Darya A Meshalkina, Erin E Frick, David J Echevarria, Denis B Rosemberg, Caio Maximino, Monica Gomes Lima, Murilo S Abreu, Ana C Giacomini, Leonardo J G Barcellos, Cai Song, Allan V Kalueff
Modeling of stress and anxiety in adult zebrafish (Danio rerio) is increasingly utilized in neuroscience research and central nervous system (CNS) drug discovery. Representing the most commonly used zebrafish anxiety models, the novel tank test (NTT) focuses on zebrafish diving in response to potentially threatening stimuli, whereas the light-dark test (LDT) is based on fish scototaxis (innate preference for dark vs. bright areas). Here, we systematically evaluate the utility of these two tests, combining meta-analyses of published literature with comparative in vivo behavioral and whole-body endocrine (cortisol) testing...
May 1, 2017: Zebrafish
https://www.readbyqxmd.com/read/28422577/enrichment-of-zebrafish-danio-rerio-hamilton-1822-diet-with-polyunsaturated-fatty-acids-improves-fecundity-and-larvae-quality
#6
Joanna Nowosad, Dariusz Kucharczyk, Katarzyna Targońska
The zebrafish is a freshwater fish of the Cyprinidae family, which is frequently used in scientific research. It owes its popularity to its genome, whose structure is comparable to the human genome and, for this reason, this species is often used in human medical research. However, such research requires high-quality material to conduct tests producing repeatable results. This study examines the effect of providing feed enriched with essential fatty acids, especially docosahexaenoic acid, to zebrafish spawners on fertility as well as the survival rate and growth of their offspring...
April 19, 2017: Zebrafish
https://www.readbyqxmd.com/read/28394248/highest-diploid-number-among-gymnotiformes-first-cytogenetic-insights-into-rhabdolichops-sternopygidae
#7
Pablo Suárez, Inaê Cristina Guerreiro Pinto Barroso, Danillo Dos Santos Silva, Susana S R Milhomem, Diogo Cavalcanti Cabral-de-Mello, Cesar Martins, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi
We report the first comparative cytogenetic analysis of two species from electrogenic fish of genus Rhabdolichops (Sternopygidae, Gymnotiformes): Rhabdolichops troscheli and Rhabdolichops cf eastwardi. R. troscheli has 2n = 54 (fundamental number [FN] = 66), whereas R. cf. eastwardi has 2n = 74 (FN = 78). C-banding revealed centromeric constitutive heterochromatin in both species. Ag-NORs mapped on pair 6 in R. troscheli and pair 30 in R. cf eastwardi. Fluorescense in situ hybridization with 18S rDNA probes confirmed the Ag-NOR staining results and revealed additional (presumably silent) ribosomal genes on pairs 12, 13, 21, 23, 26, and 27 in R...
April 10, 2017: Zebrafish
https://www.readbyqxmd.com/read/28394244/a-zebrafish-model-of-human-fibrodysplasia-ossificans-progressiva
#8
Melissa LaBonty, Nicholas Pray, Pamela C Yelick
Fibrodysplasia ossificans progressiva (FOP) is a rare, autosomal dominant genetic disorder in humans characterized by explosive inflammatory response to injury leading to gradual ossification within fibrous tissues, including skeletal muscle, tendons, and ligaments. A variety of animal models are needed to study and understand the etiology of human FOP. To address this need, here we present characterizations of the first adult zebrafish model for FOP. In humans, activating mutations in the Type I BMP/TGFβ family member receptor, ACVR1, are associated with FOP...
April 10, 2017: Zebrafish
https://www.readbyqxmd.com/read/28355106/chromosomal-mapping-of-repetitive-dna-sequences-in-the-genus-bryconamericus-characidae-and-dna-barcoding-to-differentiate-populations
#9
Angélica Rossotti Dos Santos, Mariana Campaner Usso, Juceli Gonzalez Gouveia, Cristian Araya-Jaime, Wilson Frantine-Silva, Lucia Giuliano-Caetano, Fausto Foresti, Ana Lúcia Dias
The mapping of repetitive DNA sites by fluorescence in situ hybridization has been widely used for karyotype studies in different species of fish, especially when dealing with related species or even genera presenting high chromosome variability. This study analyzed three populations of Bryconamericus, with diploid number preserved, but with different karyotype formulae. Bryconamericus ecai, from the Forquetinha river/RS, presented three new cytotypes, increasing the number of karyotype forms to seven in this population...
March 29, 2017: Zebrafish
https://www.readbyqxmd.com/read/28350247/the-transgenic-zebrafish-display-fluorescence-reflecting-the-expressional-dynamics-of-dihydrofolate-reductase
#10
Wen-Ni Chang, Wan-Yu Chi, Tseng-Ting Kao, Jen-Ning Tsai, Wangta Liu, Shih-Shin Liang, Chien-Chih Chiu, Bing-Hung Chen, Tzu-Fun Fu
Dihydrofolate reductase (DHFR) reduces folic acid and recycles dihydrofolate generated during dTMP biosynthesis to tetrahydrofolate. DHFR is upregulated in rapidly proliferating cells and hence a favored target of antifolate drug against cancers, autoimmune diseases, and microbial infections. However, increased expression of dhfr contributed to the often emerging drug resistance and impeded the therapeutic efficacy of antifolate drugs. Therefore, comprehensive knowledge on the expressional control of dhfr becomes crucial...
March 28, 2017: Zebrafish
https://www.readbyqxmd.com/read/28318435/determining-zebrafish-epitope-reactivity-to-commercially-available-antibodies
#11
Michael A Villarreal, Nicole M Biediger, Natalie A Bonner, Jennifer N Miller, Samantha K Zepeda, Benjamin J Ricard, Dana M García, Karen A Lewis
Antibodies raised against mammalian proteins may exhibit cross-reactivity with zebrafish proteins, making these antibodies useful for fish studies. However, zebrafish may express multiple paralogues of similar sequence and size, making them difficult to distinguish by traditional Western blot analysis. To identify the zebrafish proteins that are recognized by an antimammalian antibody, we developed a system to screen putative epitopes by cloning the sequences between the yeast SUMO protein and a C-terminal 6xHis tag...
March 20, 2017: Zebrafish
https://www.readbyqxmd.com/read/28318417/itfish-enhanced-staining-by-iterative-fluorescent-in-situ-hybridization
#12
Richard H Row, Benjamin L Martin
Fluorescent in situ hybridization (FISH) is an important tool for zebrafish research, particularly when observing the expression of two different genes in the same embryo. Peroxidase-catalyzed deposition of tyramide-conjugated dyes is a widely used and cost-effective approach to performing FISH. A major limitation of the technique is that it does not work well for weakly expressed genes. Here we present a method adapted from planarian research for use in zebrafish that provides a dramatic enhancement of weak staining...
March 20, 2017: Zebrafish
https://www.readbyqxmd.com/read/28318412/effects-of-lithium-and-2-4-dichlorophenol-on-zebrafish-circadian-rhythm-disorder-and-molecular-effects
#13
Bo Xiao, Li-Qiang Cui, Cheng Ding, Han Wang
The aim of this study was to investigate lithium and 2,4-dichlorophenol (2,4-DCP)-induced circadian rhythm disorder and their genome-wide effects in zebrafish. Zebrafish larvae were exposed to 250 ppm LiCl (n = 40) or 20 ppm 2,4-DCP. RNA was subsequently extracted and determined quantitatively. The mRNA levels of circadian clock-related genes, including clock1a, bmal1b, per2, and per1b, were determined. Microarray datasets were generated and the differentially expressed genes (DEGs) were identified. The mRNA levels of some upregulated and downregulated DEGs were examined by quantitative real-time polymerase chain reaction (RT-PCR)...
March 20, 2017: Zebrafish
https://www.readbyqxmd.com/read/28296621/optical-projection-tomography-as-a-novel-method-to-visualize-and-quantitate-whole-brain-patterns-of-cell-proliferation-in-the-adult-zebrafish-brain
#14
Benjamin W Lindsey, Jan Kaslin
How distinct cell populations are distributed in three-dimensional space under homeostasis or following injury, neurodegeneration, or with senescence can teach us much about brain-wide patterns and signaling along the neuroaxis. Visualizing individual cell populations in the mature vertebrate central nervous system (CNS) has remained a challenge as a result of difficulty clearing adult brain tissue or limitations in imaging depth or resolution. We have developed a simple clearing and imaging pipeline optimally suited for the adult zebrafish brain to investigate changes in patterns of cell proliferation in wild-type and transgenic backgrounds that can easily be quantified and represented using FIJI and IMARIS software...
March 15, 2017: Zebrafish
https://www.readbyqxmd.com/read/28287927/three-dimensional-printed-fish-graders-a-tool-to-rapidly-and-reliably-size-select-zebrafish
#15
Thomas O'Reilly-Pol, Kyle Kniepkamp, Stephen L Johnson
Research into adult zebrafish often requires fish to be of a specific size. Currently, fish must be individually measured to achieve this goal. Here, we design and utilize fish graders to quickly sort fish by width. We characterize graders individually for the length of fish they discriminate between and we also analyze graders in pairs to define the range of lengths for a retained population of fish. We note that a 1 mm increase of fish width increases fish length by 6.2-7.2 mm. We provide the schematics to print a series of eight retention widths, and note that graders of any desired retention width can easily be printed by slightly modifying our design files...
March 13, 2017: Zebrafish
https://www.readbyqxmd.com/read/28277948/karyotypic-evolution-and-chromosomal-organization-of-repetitive-dna-sequences-in-species-of-panaque-panaqolus-and-scobinancistrus-siluriformes-and-loricariidae-from-the-amazon-basin
#16
Thayana Ayres-Alves, Adauto Lima Cardoso, Cleusa Yoshiko Nagamachi, Leandro Melo de Sousa, Julio Cesar Pieczarka, Renata Coelho Rodrigues Noronha
Loricariidae family comprises the greatest variability of Neotropical catfish species, with more than 800 valid species. This family shows significant chromosomal diversity. Mapping of repetitive DNA sequences can be very useful in exploring such diversity, especially among groups that appear to share a preserved karyotypic macrostructure. We describe the karyotypes of Panaque armbrusteri and Panaqolus sp., as assessed using classical cytogenetic methods. Moreover, we offer a map of their repetitive sequences, including 18S and 5S ribosomal DNAs, the Rex1 and Rex3 retrotransposons, and the Tc1-mariner transposon in P...
March 9, 2017: Zebrafish
https://www.readbyqxmd.com/read/28266909/exposure-to-1-2-propanediol-impacts-early-development-of-zebrafish-danio-rerio-and-induces-hyperactivity
#17
Andrey Massarsky, Ayham Abdel, Lilah Glazer, Edward D Levin, Richard T Di Giulio
The use of electronic cigarettes (e-cigarettes) is increasing as an alternative to tobacco burning cigarettes; however, their safety remains to be fully determined. The long-term effects of e-cigarettes are unknown, including the effects of maternal e-cigarette use on pre- and postnatal development. Additional research on the safety of e-cigarettes is needed. Especially useful would be information from high- and moderate-throughput economic model systems. This study investigates the effects of 1,2-propanediol, which was identified as the main component of e-cigarette liquid, on early development of zebrafish (an in vivo high-throughput model system that was recently proposed for the study of tobacco cigarette and e-cigarette toxicity)...
March 7, 2017: Zebrafish
https://www.readbyqxmd.com/read/28437173/the-karyotype-of-microsternarchus-aff-bilineatus-a-first-case-of-y-chromosome-degeneration-in-gymnotiformes
#18
Jéssica Almeida Batista, Adauto Lima Cardoso, Susana Suely Rodrigues Milhomem Paixão, Jonathan Stuart Ready, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi
Various species and lineages that until recently were identified as Microsternarchus bilineatus (Hypopomidae, Gymnotiformes) have a widespread distribution in the Amazon and Orinoco river basins and across the Guiana shield. Recent molecular studies show five distinct lineages for Microsternarchus from different localities. These results suggest that this previously monotypic genus actually consists of more than one species. Here, we describe the karyotype of M. aff. bilineatus from the Cururutuia River (Bragança, Pará, Brazil)...
March 6, 2017: Zebrafish
https://www.readbyqxmd.com/read/28192066/performance-of-debaryomyces-hansenii-as-a-diet-for-rotifers-for-feeding-zebrafish-larvae
#19
Rafael Opazo, Karen Fuenzalida, Francisca Plaza-Parrochia, Jaime Romero
The zebrafish larval stage is a critical moment due to high mortality rates associated with inadequate supplies of nutritional requirements. Larval feeding has important challenges associated with such factors as small mouth gape (≈100 μm), the low activity of digestive enzymes, and the intake of live food. A common zebrafish live food at the onset of exogenous feeding is rotifers, mainly Brachionus plicatilis. These rotifers should be fed with other microorganisms such as microalgae or yeast, mostly from the Saccharomyces genus...
April 2017: Zebrafish
https://www.readbyqxmd.com/read/28186458/correction-to-zebrafish-2016-13-545-547
#20
(no author information available yet)
No abstract text is available yet for this article.
April 2017: Zebrafish
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