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International Journal of Medical Sciences

Hsiang-Hao Chuang, Ming-Shyan Huang, Pei-Hui Wang, Yu-Peng Liu, Michael Hsiao, Chih-Jen Yang
Histone deacetylase 6 (HDAC6), a member of the HDAC enzymes, has been reported to play substantial roles in many cellular processes. Evidence shows that deregulation of HDAC6 may be involved in the progression of some cancers, neurodegenerative diseases, and inflammatory disorders. However, little is known regarding the effect of post-translational modification of HDAC6 on cellular localization and biological functions. In the present study, we identified four phosphorylation sites on HDAC6 under normal conditions by mass spectrometry analysis...
2018: International Journal of Medical Sciences
Ning Li, Zhang-Sheng Wang, Xin-Hua Wang, Ying-Jia Xu, Qi Qiao, Xiu-Mei Li, Ruo-Min Di, Xiao-Juan Guo, Ruo-Gu Li, Min Zhang, Xing-Biao Qiu, Yi-Qing Yang
Atrial fibrillation (AF), as the most common sustained cardiac arrhythmia, is associated with substantially increased morbidity and mortality. Aggregating evidence demonstrates that genetic defects play a crucial role in the pathogenesis of AF, especially in familial AF. Nevertheless, AF is of pronounced genetic heterogeneity, and in an overwhelming majority of cases the genetic determinants underlying AF remain elusive. In the current study, 162 unrelated patients with familial AF and 238 unrelated healthy individuals served as controls were recruited...
2018: International Journal of Medical Sciences
Xianliang Dai, Danfeng Zhang, Chaoqun Wang, Zonggui Wu, Chun Liang
Atherosclerosis is one kind of chronic inflammatory disease, in which multiple types of immune cells or factors are involved. Data from experimental and clinical studies on atherosclerosis have confirmed the key roles of immune cells and inflammation in such process. The thymus as a key organ in T lymphocyte ontogenesis has an important role in optimizing immune system function throughout the life, and dysfunction of thymus has been proved to be associated with severity of atherosclerosis. Based on previous research, we begin with the hypothesis that low density lipoprotein or cholesterol reduces the expression of the thymus transcription factor Foxn1 via low density lipoprotein receptors on the membrane surface and low density lipoprotein receptor related proteins on the cell surface, which cause the thymus function decline or degradation...
2018: International Journal of Medical Sciences
Shumin Xie, Xiaoyu Liu, Zhen Pan, Xing Chen, Anquan Peng, Tuanfang Yin, Jihao Ren, Wei Liu
Objectives: To analyze the miRNAs expression profiling between acquired middle ear cholesteatoma and normal skin, and to identify several novel miRNAs which may be involved in the etiopathogenesis of middle ear cholesteatoma. Methods: MiRNA microarray technology was adopted to analyze the miRNA expression profiling between acquired middle ear cholesteatoma and normal skin. qRT-PCR was used to validate selected differentially expressed miRNAs. Results: The miRNA microarray technology showed totally 44 upregulated (miRNA-21-3p, miRNA-584-5p, miRNA-16-1-3p, etc) and 175 downregulated (miRNA-10a-5p, miRNA-152-5p, miRNA-203b-5p, etc) miRNAs in cholesteatoma tissues with 2-fold change compared with normal skin...
2018: International Journal of Medical Sciences
Chih-Chung Feng, Po-Hsiang Liao, Hsiang-I Tsai, Shiu-Min Cheng, Liang-Yo Yang, Vijaya PadmaViswanadha, Lung-Fa Pan, Ray-Jade Chen, Jeng-Fan Lo, Chih-Yang Huang
Dilated cardiomyopathy (DCM) is the most common form of non-ischemic cardiomyopathy. It is characterized by ventricular chamber dilation, and myocyte hypertrophy. Human tumorous imaginal disc 1 (Tid1), a chaperone protein and response to regulate number of signaling molecules in the mitochondria or cytosol. Tid1 also plays a major role in preventing DCM; however, the role of Tid1 in isoproterenol (ISO)-induced cardiac apoptosis and hypertrophy remains unclear. H9c2 cells were pretreated Tid1 before ISO-induced hypertrophy and apoptosis and then evaluated by IHC, TUNEL assay, IFC, Co-IP, and Western blot...
2018: International Journal of Medical Sciences
Jeong Min Moon, Youn-Jee Chung, Boah Chae, Hee Jin Kang, Hyun Hee Cho, Jang Heub Kim, Mee-Ran Kim
Research Question: To evaluate the effect of mistletoe on the cell viability of patients with endometriosis, the expression levels of vascular endothelial growth factor (VEGF) were measured, and the change in the expression level of VEGF following mistletoe treatment was recorded. Design: Forty reproductive-aged women with endometriosis (stage I/II [group 1, n=20], and stage III/IV [group 2, n=20]) were prospectively enrolled. Twenty women who underwent gynaecologic operations for benign conditions were selected as the control group...
2018: International Journal of Medical Sciences
Min-Soo Kim, Sarah Soh, So Yeon Kim, Min Sup Song, Jin Ha Park
Background: During robot-assisted laparoscopic radical prostatectomy (RALP), steep Trendelenburg position and carbon dioxide pneumoperitoneum are inevitable for surgical exposure, both of which can impair cardiopulmonary function. This study was aimed to compare the effects of pressure-controlled ventilation with volume guarantee (PCV with VG) and 1:1 equal ratio ventilation (ERV) on oxygenation, respiratory mechanics and hemodynamics during RALP. Methods: Eighty patients scheduled for RALP were randomly allocated to either the PCV with VG or ERV group...
2018: International Journal of Medical Sciences
Xiaotong Wang, Yunyun Li, Yan Mou, Zhen Yue, Haiying Zhang, Ronggui Li, Hongxia Sun
The present study aimed to investigate the effects of ethanol extract from Brucea javanica seed (EEBJS) on the angiogenesis of human umbilical vein endothelial cells (HUVECs) and the possible molecular signal involved. Firstly, a Matrigel-based in vitro angiogenesis assay demonstrated that EEBJS inhibited the angiogenesis of HUVECs in a dose-dependent manner. Then by using porcine aortic endothelial cells which stably express human PDGFR - beta, we found that the inhibition of angiogenesis was mediated by PDGFR - beta...
2018: International Journal of Medical Sciences
Feifei Su, Miaoqian Shi, Jian Zhang, Qiangsun Zheng, Dongwei Zhang, Wei Zhang, Haichang Wang, Xue Li
Cardiac hypertrophy is an independent predictor of cardiovascular morbidity and mortality. To identify the mechanisms by which simvastatin inhibits cardiac hypertrophy induced by pressure overload, we determined effects of simvastatin on 14-3-3 protein expression and autophagic activity. Simvastatin was administered intragastrically to Sprague-Dawley (SD) rats before abdominal aortic banding (AAB). Neonatal rat cardiomyocytes (NRCs) were treated with simvastatin before angiotensin II (AngII) stimulation. 14-3-3, LC3, and p62 protein levels were determined by western blot...
2018: International Journal of Medical Sciences
Aolin Du, Yufeng Jiang, Chuifeng Fan
N-myc downstream regulated gene 1 (NDRG1) plays a variety of roles in human cancers. Our previous studies showed that NDRG1 expression is elevated in non-small cell lung cancer and contributes to cancer growth. However, its function in apoptosis and chemoresistance in malignant tumors, including lung cancer, is not yet fully understood. In this study, we investigated the roles of NDRG1 in chemoresistance to cisplatin in lung cancer cells. We found that overexpression of NDRG1 significantly reduced cisplatin-induced cytotoxicity in lung cancer A549 cells, while overexpression of activating transcription factor 3 (ATF3), a stress-inducible gene found to be associated with apoptosis in some human cancers, significantly promoted cytotoxicity ( P < 0...
2018: International Journal of Medical Sciences
Federica Facchin, Eva Bianconi, Miriam Romano, Alessia Impellizzeri, Francesco Alviano, Margherita Maioli, Silvia Canaider, Carlo Ventura
Human Mesenchymal Stem Cells (hMSCs) undergo senescence in lifespan. In most clinical trials, hMSCs experience long-term expansion ex vivo to increase cell number prior to transplantation, which unfortunately leads to cell senescence, hampering post-transplant outcomes. Hydrogen peroxide (H2 O2 ) in vitro represents a rapid, time and cost-effective tool, commonly used as oxidative stress tantalizing the stem cell ability to cope with a hostile environment, recapitulating the onset and progression of cellular senescence...
2018: International Journal of Medical Sciences
Peng Wang, Yanming Cao, Dongxiang Zhan, Ding Wang, Bin Wang, Yamei Liu, Gang Li, Wei He, Haibin Wang, Liangliang Xu
Purpose: A key factor in regulating bone absorption is the proportion of RANKL/OPG. Although many reports showing diverse transcription factors or epigenetic modification could be responsible for regulating RANKL&OPG ratio, there is still little exploration on promoter methylation status of both genes in osteoporotic bone tissues. Our aim is to investigate the changes of methylation in CpG island of these genes' promoters in patients with primary osteoporosis. Methods: The diagnosis of osteoporosis was based on the results of dual energy X-ray absorptiometry measurements...
2018: International Journal of Medical Sciences
Yun Peng, Zhang Liang, Aihua Liu, Erhua Li, Xiting Dai, Ruolan Bai, Zhenhua Ji, Miaomiao Jian, Mingbiao Ma, Lvyan Tao, Fukai Bao, Feng Wang, YunFeng Bi, Zhe Ding, Abi Manzama-Esso
In this study, we investigated the mechanisms that lead to the production of proinflammatory mediators by the murine macrophage cell line, RAW264.7, when these cells are exposed in vitro to recombinant Borrelia burgdorferi basic membrane protein A (rBmpA). Using antibody protein microarray technology with high-throughput detection ability for detecting 25 chemokines in culture supernatant the RAW264.7 cell culture supernatants at 12 and 24 h post-stimulation with rBmpA, we identified two chemokines, a monocyte chemoattractant protein-5 (MCP-5/CCL12) and a macrophage inflammatory protein-2 (MIP-2/CXCL2), both of which increased significantly after stimulation...
2018: International Journal of Medical Sciences
Guanglin Chen, Lei Peng, Zhongxian Zhu, Chunxia Du, Ziyang Shen, Rujin Zang, Yang Su, Yankai Xia, Weibing Tang
[This corrects the article DOI: 10.7150/ijms.18392.].
2018: International Journal of Medical Sciences
Abdulkadir Burak Cankaya, Metin Berk Kasapoglu, Mehmet Ali Erdem, Cetin Kasapoglu
Aim: Malfixed miniplates can impair fracture healing, and the screw pilot holes may widen during repeated fixation trials. This in vitro study explored the extent to which screw fixation of mandibular angle fractures could be improved by augmenting the drilling holes with polymethylmethacrylate (PMMA). Materials and Methods: We measured stabilization by recording specimen displacement under a vertical force of 50 N applied using a hydraulic tester. We included 20 hemimandibles from sheep (average weight 40 kg)...
2018: International Journal of Medical Sciences
Guo-Xin Qu, Zhi-Min Ying, Chen-Chen Zhao, Shi-Gui Yan, Xun-Zi Cai
Aseptic loosening is the most common complication of joint replacement. Previous studies showed that acrylic bone cement loaded with a commercially-available alendronate powder (APAC) had good promise against wear debris-mediated osteolysis for prevention of aseptic loosening. The purpose of the present study was to investigate the effect of adding alendronate powder to an acrylic bone cement on quasi-static mechanical properties (namely, compressive strength, compressive modulus, tensile strength, and flexural strength), fatigue life, porosity, and microstructure of the cement...
2018: International Journal of Medical Sciences
Abel Olusola Idowu, Sanjib Bhattacharyya, Steve Gradus, Wellington Oyibo, Zenas George, Carolyn Black, Joseph Igietseme, Anthony Ajayi Azenabor
The artemisinin-based combined therapy (ACT) post-treatment illness in Plasmodium falciparum -endemic areas is characterized by vague malaria-like symptoms. The roles of treatment modality, persistence of parasites and host proinflammatory response in disease course are unknown. We investigated the hypothesis that ACT post-treatment syndrome is driven by parasite genetic polymorphisms and proinflammatory response to persisting mutant parasites. Patients were categorized as treated, untreated and malaria-negative...
2018: International Journal of Medical Sciences
Xinju Zhang, Yanli Li, Pengfei Qi, Zhongliang Ma
MicroRNAs, a class of short endogenous RNAs, acting as post-transcriptional regulators of gene expression, mostly silence gene expression via binding imperfectly matched sequences in the 3'UTR of target mRNA. MiR-17-92, a highly conserved gene cluster, has 6 members including miR-17, miR-18a, miR-19a, miR-20a, miR-19b-1 and miR-92a. The miR-17-92 cluster, regarded as oncogene, is overexpressed in human cancers. Lung cancer is the leading cause of death all over the world. The molecular mechanism of lung cancer has been partly known at the levels of genes and proteins in last decade...
2018: International Journal of Medical Sciences
Mei Yang, Yang-Yang Zhuang, Wei-Wei Wang, Hai-Ping Zhu, Yan-Jie Zhang, Sao-Ling Zheng, Yi-Rrong Yang, Bi-Cheng Chen, Peng Xia, Yan Zhang
Renal tubule cell apoptosis plays a pivotal role in the progression of chronic renal diseases. The previous study indicates that Sirolimus is effective on unilateral ureteral obstruction (UUO)-induced renal fibrosis. However, the role of Sirolimus in renal tubular apoptosis induced by UUO has not yet been addressed. The aim of this study was to determine the role of Sirolimus in renal tubular apoptosis induced by UUO. Male Sprague-Dawley rats were divided into three groups, sham-operated rats, and after which unilateral ureteral obstruction (UUO) was performed: non-treated and sirolimus-treated (1mg/kg)...
2018: International Journal of Medical Sciences
Shuai An, Meng Zhou, Zheng Li, Mingli Feng, Guanglei Cao, Shibao Lu, Limin Liu
Peripheral nerve injury is known to activate the hypoxia-inducible factor-1α (HIF-1α) pathway as one of pro-regenerative transcriptional programs, which could stimulate multiple injury-induced gene expression and contribute to axon regeneration and functional recovery. However, the role of HIF-1α in peripheral nerve regeneration remains to be fully elucidated. In this study, rats were divided into three groups and treated with sham surgery, surgery with cobalt chloride (CoCl2 ) and surgery with saline, respectively...
2018: International Journal of Medical Sciences
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