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Protein Journal

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https://www.readbyqxmd.com/read/29785642/differential-binding-of-active-and-inactive-integrin-to-talin
#1
Dongchuan Wang, Qiang Guo, Ailin Wei, Ang Li
Bi-directional signaling of integrins plays an important role in platelet and leukocyte function. Talin plays a key role in integrin bi-directional signaling and its binding to integrin is highly regulated. The precise regulation of the recruitment and binding of talin to integrin is still being elucidated. In particular, the recruitment of talin to integrin is controlled by the RAP-1 and RIAM/lamellipodin signaling axis and the affinity between talin and integrin is regulated by the conformation or protease cleavage of talin...
May 21, 2018: Protein Journal
https://www.readbyqxmd.com/read/29785641/in-silico-analysis-of-natural-resistance-associated-macrophage-protein-nramp-family-of-transporters-in-rice
#2
Anitha Mani, Kavitha Sankaranarayanan
In Oryza sativa (rice) there are seven members in the NRAMP (natural resistance- associated macrophage protein) family of transporter proteins. They have been identified as OsNRAMP1, OsNRAMP2, OsNRAMP3, OsNRAMP4, OsNRAMP5, OsNRAMP6 and OsNRAMP7. Several metal ions like Zn2+ , Mn2+ , Fe2+ , Cd2+ etc. have been studied to be transported via NRAMP transporter proteins in rice plant. In spite of this, very little information is available regarding these transporters. Hence it is important to computationally predict and characterize the OsNRAMP family of transporters for studying and understanding their molecular insights in future studies...
May 21, 2018: Protein Journal
https://www.readbyqxmd.com/read/29779193/functional-role-of-tyr12-in-the-catalytic-activity-of-novel-zeta-like-glutathione-s-transferase-from-acidovorax-sp-kks102
#3
Dayyabu Shehu, Zazali Alias
Glutathione S-transferases (GSTs) are a family of enzymes that function in the detoxification of variety of electrophilic substrates. In the present work, we report a novel zeta-like GST (designated as KKSG9) from the biphenyl/polychlorobiphenyl degrading organism Acidovorax sp. KKS102. KKSG9 possessed low sequence similarity but similar biochemical properties to zeta class GSTs. Functional analysis showed that the enzyme exhibits wider substrate specificity compared to most zeta class GSTs by reacting with 1-chloro-2,4-dinitrobenzene (CDNB), p-nitrobenzyl chloride (NBC), ethacrynic acid (EA), hydrogen peroxide, and cumene hydroperoxide...
May 19, 2018: Protein Journal
https://www.readbyqxmd.com/read/29761378/inhibition-and-substrate-specificity-properties-of-fkbp22-from-a-psychrotrophic-bacterium-shewanella-sp-sib1
#4
Cahyo Budiman, Herman Umbau Lindang, Bo Eng Cheong, Kenneth F Rodrigues
SIB1 FKBP22 is a peptidyl prolyl cis-trans isomerase (PPIase) member from a psychrotrophic bacterium, Shewanella sp. SIB1, consisting of N- and C-domains responsible for dimerization and catalytic PPIase activity, respectively. This protein was assumed to be involved in cold adaptation of SIB1 cells through its dual activity of PPIase activity and chaperone like-function. Nevertheless, the catalytic inhibition by FK506 and its substrate specificity remain unknown. Besides, ability of SIB1 FKBP22 to inhibit phosphatase activity of calcinuerin is also interesting to be studied since it may reflect wider cellular functions of SIB1 FKBP22...
May 14, 2018: Protein Journal
https://www.readbyqxmd.com/read/29691701/the-use-of-human-bovine-and-camel-milk-albumins-in-anticancer-complexes-with-oleic-acid
#5
Esmail M El-Fakharany, Marwa M Abu-Serie, Ekaterina A Litus, Sergei E Permyakov, Eugene A Permyakov, Vladimir N Uversky, Elrashdy M Redwan
Oleic acid (OA) is a monounsaturated fatty acid that upon binding to milk proteins, such as α-lactalbumin and lactoferrin, forms potent complexes, which exert selective anti-tumor activity against malignant cells but are nontoxic for healthy normal cells. We showed that the interaction of OA with albumins isolated from human, bovine, and camel milk results in the formation of complexes with high antitumor activity against Caco-2, HepG-2, PC-3, and MCF-7 tumor cells. The antitumor effect of the complexes is mostly due to the action of oleic acid, similar to the case of OA complexes with other proteins...
April 24, 2018: Protein Journal
https://www.readbyqxmd.com/read/29666988/obituary-g%C3%A3-nter-blobel
#6
(no author information available yet)
No abstract text is available yet for this article.
April 17, 2018: Protein Journal
https://www.readbyqxmd.com/read/29651717/alcohol-metabolic-inefficiency-structural-characterization-of-polymorphism-induced-aldh2-dysfunctionality-and-allosteric-site-identification-for-design-of-potential-wildtype-reactivators
#7
Emmanuel A Adeniji, Fisayo A Olotu, Mahmoud E S Soliman
Liver mitochondrial aldehyde dehydrogenase 2 (ALDH2) enzyme is responsible for the rapid conversion of acetaldehyde to acetic acid. ALDH2 (E487K) polymorphism results in an inactive allele (ALDH2*2) which cause dysfunctional acetaldehyde metabolism. The 3D structure of an enzyme is crucial to its functionality and a disruption in its structural integrity could result in its metabolic inefficiency and dysfunctionality. Allosteric targeting of polymorphs could facilitate the restoration of wildtype functionalities in ALDH2 polymorphs and serve as an advancement in the treatment of associated diseases...
April 12, 2018: Protein Journal
https://www.readbyqxmd.com/read/29651716/super-secondary-structure-consisting-of-a-polyproline-ii-helix-and-a-%C3%AE-turn-in-leucine-rich-repeats-in-bacterial-type-iii-secretion-system-effectors
#8
Dashdavaa Batkhishig, Khurelbaatar Bilguun, Purevjav Enkhbayar, Hiroki Miyashita, Robert H Kretsinger, Norio Matsushima
Leucine rich repeats (LRRs) are present in over 100,000 proteins from viruses to eukaryotes. The LRRs are 20-30 residues long and occur in tandem. LRRs form parallel stacks of short β-strands and then assume a super helical arrangement called a solenoid structure. Individual LRRs are separated into highly conserved segment (HCS) with the consensus of LxxLxLxxNxL and variable segment (VS). Eight classes have been recognized. Bacterial LRRs are short and characterized by two prolines in the VS; the consensus is xxLPxLPxx with Nine residues (N-subtype) and xxLPxxLPxx with Ten residues (T-subtype)...
April 12, 2018: Protein Journal
https://www.readbyqxmd.com/read/29564676/the-catalytically-inactive-mutation-of-the-ubiquitin-conjugating-enzyme-cdc34-affects-its-stability-and-cell-proliferation
#9
Xun Liu, Yang Zhang, Zhanhong Hu, Qian Li, Lu Yang, Guoqiang Xu
The ubiquitin proteasome system (UPS) plays important roles in the regulation of protein stability, localization, and activity. A myriad of studies have focused on the functions of ubiquitin ligases E3s and deubiquitinating enzymes DUBs due to their specificity in the recognition of downstream substrates. However, the roles of the most ubiquitin-conjugating enzymes E2s are not completely understood except that they transport the activated ubiquitin and form E2-E3 protein complexes. Ubiquitin-conjugating enzyme CDC34 can promote the degradation of downstream targets through the UPS whereas its non-catalytic functions are still elusive...
March 21, 2018: Protein Journal
https://www.readbyqxmd.com/read/29549627/substitution-of-alanine-at-position-184-with-glutamic-acid-in-escherichia-coli-pbp5-%C3%AE-like-loop-introduces-a-moderate-cephalosporinase-activity
#10
Debasish Kar, Satya Deo Pandey, Sathi Mallick, Mouparna Dutta, Anindya S Ghosh
Escherichia coli PBP5, a DD-carboxypeptidase (DD-CPase), helps in maintaining cell shape and intrinsic β-lactam resistance. Though PBP5 does not have β-lactamase activity under physiological pH, it has a common but shorter Ω-like loop resembling class A β-lactamases. However, such Ω-like loop lacks the key glutamic acid residue that is present in β-lactamases. It is speculated that β-lactamases and DD-CPases might have undergone divergent evolution leading to distinct enzymes with different substrate specificities and functions indicating the versatility of the Ω-loops...
April 2018: Protein Journal
https://www.readbyqxmd.com/read/29508210/biochemical-characterization-of-the-cytochrome-p450-cyp107cb2-from-bacillus-lehensis-g1
#11
Swi See Ang, Abu Bakar Salleh, Leow Thean Chor, Yahaya M Normi, Bimo Ario Tejo, Mohd Basyaruddin Abdul Rahman, Mariam-Aisha Fatima
The bioconversion of vitamin D3 catalyzed by cytochrome P450 (CYP) requires 25-hydroxylation and subsequent 1α-hydroxylation to produce the hormonal activated 1α,25-dihydroxyvitamin D3. Vitamin D3 25-hydroxylase catalyses the first step in the vitamin D3 biosynthetic pathway, essential in the de novo activation of vitamin D3. A CYP known as CYP107CB2 has been identified as a novel vitamin D hydroxylase in Bacillus lehensis G1. In order to deepen the understanding of this bacterial origin CYP107CB2, its detailed biological functions as well as biochemical characteristics were defined...
April 2018: Protein Journal
https://www.readbyqxmd.com/read/29508209/expression-purification-and-characterization-of-hiv-1-capsid-precursor-protein-p41
#12
Zhiqing Zhang, Lei Wang, Shimeng Bai, Jiaming Qiao, Honglin Shen, Fang Huang, Shuangquan Gao, Shaoyong Li, Shaowei Li, Ying Gu, Ningshao Xia
Human immunodeficiency virus type 1 (HIV-1) has been a global epidemic since 1983; yet, the virology and immunology related to HIV-1 remain elusive. Furthermore, as there is still no effective chemoprophylaxis or vaccine to treat patients with HIV-1, most research focuses on strategies to prevent HIV-1 infection, such as with antiviral drugs, novel therapeutics, or improved diagnostic kits. The HIV-1 Gag precursor protein (p55)-comprising the matrix (MA/p17), capsid (CA/p24), and nucleocapsid (NC/p7) protein domains-is the main structural HIV-1 protein, and is uniquely responsible for virion assembly within the virus life cycle...
April 2018: Protein Journal
https://www.readbyqxmd.com/read/29500718/interaction-between-a-gelsolin-from-dendrorhynchus-zhejiangensis-with-three-gelsolin-like-domains-and-actin-in-vitro
#13
Ye Li, Lei Chen, Jun Zhou, Xiurong Su, Taiwu Li
The gelsolin family proteins are best known for involvement in cytoskeletal rearrangement by controlling actin organization during a variety of cellular processes. Previously, a 1962 bp cDNA encoding a 41.7 kDa protein with three gelsolin-like domains (G domains) from Dendrorhynchus zhejiangensis was identified and named as DzGSN. In this study, the sequence and function of a novel member of the gelsolin family proteins from D. zhejiangensis have been analyzed. Sequence alignment indicates that DzGSN is highly homologous to human gelsolin (35% identity) and human CapG (36% identity)...
April 2018: Protein Journal
https://www.readbyqxmd.com/read/29464485/determining-the-roles-of-a-conserved-%C3%AE-helix-in-a-global-virulence-regulator-from-staphylococcus-aureus
#14
Avisek Mahapa, Sukhendu Mandal, Debabrata Sinha, Subrata Sau, Keya Sau
SarA, a pleiotropic transcription regulator, is encoded by Staphylococcus aureus, a pathogenic bacterium. The expression of many virulence and non-virulence genes in S. aureus is modulated by this regulator. Structural studies have shown it to be a winged-helix DNA-binding protein carrying two monomers. Each SarA monomer is composed of five α-helices (α1-α5), three β-strands (β1-β3) and multiple loops. The putative DNA binding region of SarA is constituted with α3, α4, β2, and β3, whereas, its dimerization seems to occur using α1, α2, and α5...
April 2018: Protein Journal
https://www.readbyqxmd.com/read/29430595/the-c19s-substitution-enhances-the-stability-of-hepcidin-while-conserving-its-biological-activity
#15
Edina Pandur, Zsuzsanna Fekete, Kitti Tamási, László Grama, Edit Varga, Katalin Sipos
Hepcidin, the key hormone of iron homeostasis is responsible for lowering the serum iron level through its interaction with iron exporter ferroportin. Thus, hepcidin agonists provide a promising opportunity in the treatment of iron disorders caused by lacking or decreased hepcidin expression. We investigated the importance of each of the eight highly conserved cysteines for the biological activity of hepcidin. Eight cysteine mutants were created with site directed mutagenesis. The binding ability of these hepcidin mutants to the hepcidin receptor ferroportin was determined using bacterial two-hybrid system and WRL68 human hepatic cells...
April 2018: Protein Journal
https://www.readbyqxmd.com/read/29411223/in-vitro-screening-of-synthetic-fluorogenic-substrates-for-detection-of-cancer-procoagulant-activity
#16
Jason Krause, Carminita L Frost
Cancer procoagulant (CP), a direct activator of coagulation factor X, is among one of the tumour cell products or activities which may promote fibrin formation and has been suggested to be selectively associated with the malignant phenotype. At present, the most reliable assay for the quantification of CP activity is the three-stage chromogenic assay which utilises the ability of CP to activate factor X. In this assay, the activation of factor X leads to the formation of activated thrombin from prothrombin and the eventual hydrolyses of a thrombin chromogenic substrate which contains a p-nitroaniline leaving group...
April 2018: Protein Journal
https://www.readbyqxmd.com/read/29411222/physicochemical-characterization-glycosylation-pattern-and-biosimilarity-assessment-of-the-fusion-protein-etanercept
#17
Othman Montacir, Houda Montacir, Andreas Springer, Stephan Hinderlich, Fereidoun Mahboudi, Amirhossein Saadati, Maria Kristina Parr
Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1. It possesses three N- and 13 O-glycosylation sites. Due to its complex structure, an analytical challenge is facing the development and approval of biosimilars. In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals...
April 2018: Protein Journal
https://www.readbyqxmd.com/read/29468489/2018-letter-from-the-editor-in-chief
#18
EDITORIAL
Lawrence Berlilner
No abstract text is available yet for this article.
February 2018: Protein Journal
https://www.readbyqxmd.com/read/29380264/expression-and-characterization-of-recombinant-sucrose-phosphorylase
#19
Hui Zhang, Xiao Sun, Wenjie Li, Tuoping Li, Suhong Li, Motomitsu Kitaoka
SPase is widely used in the food, cosmetics, and pharmaceutical industries. Previously, a SPase gene was cloned from Bifidobacterium longum JCM1217 and constructed into Escherichia coli BL21. In this paper, its expression conditions were optimized. The results showed that several induction factors determined the expression efficiency of SPase. The initial cell density, IPTG concentration, and induction time and temperature significantly (p < 0.01) affected the total protein content and activity of expressed SPase...
February 2018: Protein Journal
https://www.readbyqxmd.com/read/29330644/hydrocarbon-stapled-antimicrobial-peptides
#20
Dorian Migoń, Damian Neubauer, Wojciech Kamysz
Antimicrobial peptides are promising candidates for anti-infective pharmaceuticals. Unfortunately, because of their low proteolytic and chemical stability, their usage is generally narrowed down to topical formulations. Until now, numerous approaches to increase peptide stability have been proposed. One of them, peptide hydrocarbon stapling, a modification based on stabilizing peptide secondary structure with a side-chain covalent hydrocarbon bridge, have been successfully applied to many peptides. Moreover, constraining secondary structure of peptides have also been proven to increase their biological activity...
February 2018: Protein Journal
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