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Acta Biochimica et Biophysica Sinica

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https://www.readbyqxmd.com/read/27932393/biological-characteristics-of-embryonic-epicardial-cells-in-vitro-correlate-with-embryonic-day
#1
Xiaoming Wei, Yulin Gao, Xiaodong Jing, Songbai Deng, Jianlin Du, Yajie Liu, Qiang She
The epicardial cell (EpiC) culture system plays an important role in investigating the specific mechanisms and signaling molecules that are involved in the development of EpiCs. From this early formation until adulthood, EpiCs undergo dynamic changes in the expression of embryonic genes that correlate with changes in the embryonic EpiC properties. The differences of embryonic EpiC properties may affect the related results of experiments in which EpiC culture system is used; however, these differences have not been explored...
December 7, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27932392/digital-gene-expression-profiling-analysis-of-dna-repair-pathways-in-colon-cancer-stem-population-of-ht29-cells
#2
Wenxue Wang, Guoxiu Zhang, Jing Yang, Huan Gu, Lei Ding, Haijing Yu, Min Yu, Qinghua Cui, Xinglai Ji, Meizhang Li
Cancer stem cells (CSCs) contribute to the relapse and development of new neoplasm lesions. While most available clinical approaches, such as chemical and radiation therapies, will kill the majority of cancer cells, they do not kill them all. Some resisting cells, like CSCs, are able to survive due to their excellent self-maintaining capabilities, even in challenging environments. In the present study, we investigated the mRNA level of DNA repair genes of colon CSCs from the HT29 cell line in response to single-strand damage and double-strand breaks, as well as the evident upregulation of key genes in base excision repair, mismatch repair, non-homologous end-joining, and homologous recombination pathways in these cells...
December 7, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27864283/tumor-necrosis-factor-%C3%AE-a-key-contributor-to-intervertebral-disc-degeneration
#3
REVIEW
Cheng Wang, Xiaohua Yu, Yiguo Yan, Wei Yang, Shujun Zhang, Yongxiao Xiang, Jian Zhang, Wenjun Wang
Intervertebral disc (IVD) degeneration (IDD) is the most common cause leading to low back pain (LBP), which is a highly prevalent, costly, and crippling condition worldwide. Current treatments for IDD are limited to treat the symptoms and do not target the pathophysiology. Tumor necrosis factor-α (TNF-α) is one of the most potent pro-inflammatory cytokines and signals through its receptors TNFR1 and TNFR2. TNF-α is highly expressed in degenerative IVD tissues, and it is deeply involved in multiple pathological processes of disc degeneration, including matrix destruction, inflammatory responses, apoptosis, autophagy, and cell proliferation...
November 17, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27864282/bxb1-integrase-serves-as-a-highly-efficient-dna-recombinase-in-rapid-metabolite-pathway-assembly
#4
Xianwei Wang, Biao Tang, Yu Ye, Yayi Mao, Xiaolai Lei, Guoping Zhao, Xiaoming Ding
Phage-encoded serine integrases are widely used in genetic engineering. They also have the potential to serve as efficient DNA assemblers, demonstrated by the method of site-specific recombination-based tandem assembly (SSRTA) that can combine biological parts into devices, pathways, and systems. Here, four serine integrases, ϕBT1, TG1, ϕRv1, and Bxb1, were investigated to ascertain their in vitro DNA assembly activities. Bxb1 integrase displayed the highest efficiency to obtain final products. Thus, we conclude that Bxb1 integrase is an excellent choice for DNA assembly in vitro Using this enzyme and its recognition sites, BioBrick standards were designed that are compatible with the SSRTA method for module addition...
November 17, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27864281/lipoprotein-lipase-and-phospholipid-transfer-protein-overexpression-in-human-glioma-cells-and-their-effect-on-cell-growth-apoptosis-and-migration
#5
Weijiang Dong, Huilin Gong, Guanjun Zhang, Simona Vuletic, John Albers, Jiaojiao Zhang, Hua Liang, Yanxia Sui, Jin Zheng
Glioma is one of the common tumors in brain. The expression level of lipoprotein lipase (LPL) or phospholipid transfer protein (PLTP) may influence glioma progression and its relationship with clinical and pathological parameters. The clinical significance of LPL or PLTP expression in glioma has not been established. In the present study, the LPL and PLTP levels in glioma tumors were investigated and the relationship between the LPL and PLTP level and the grade of malignant glioma was analyzed, with the aim to provide new ideas for the diagnosis and treatment of gliomas in clinical and basic research settings...
November 17, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27864280/edaravone-injection-reverses-learning-and-memory-deficits-in-a-rat-model-of-vascular-dementia
#6
Xu Li, Fen Lu, Wei Li, Lingzhi Qin, Yong Yao, Xuerong Ge, Qingkai Yu, Xinliang Liang, Dongmei Zhao, Xiaohong Li, Jiewen Zhang
Edaravone is a novel free radical scavenger that exerts neuroprotective effects by inhibiting endothelial injury and by ameliorating neuronal damage in brain ischemia. Recently, it was reported that edaravone could alleviate the pathology and cognitive deficits of Alzheimer's disease patients. However, its relevance to vascular dementia (VaD) is not clear. In this study, we partially occluded the bilateral carotid arteries of rats surgically to induce chronic cerebral hypoperfusion (CCH), a well-known rat model of VaD...
November 17, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27864279/nutrition-deficiency-promotes-apoptosis-of-cartilage-endplate-stem-cells-in-a-caspase-independent-manner-partially-through-upregulating-bnip3
#7
Zhiliang He, Luqiao Pu, Chao Yuan, Min Jia, Jian Wang
Nutrition deficiency is reported to induce apoptosis of chondrocytes and degeneration of cartilage endplate (CEP) in rabbit. Cartilage endplate stem cells (CESCs) are important for the integrity of structure and function of CEP. Bcl-2/adenovirus E1B 19-kDa-interacting protein 3 (BNIP3) has been reported to regulate apoptosis, autophagy, and cytoprotection. In this study, we aimed to determine whether nutrition deficiency induces apoptosis of CESCs, and whether or not the BNIP3-related pathway is activated in CESCs during nutrition deficiency...
November 17, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27864278/integrative-analysis-for-the-role-of-long-non-coding-rnas-in-radiation-induced-mouse-thymocytes-responses
#8
Hui Gao, Zhuo Dong, Wei Wei, Lihong Shao, Linlin Jin, Yahui Lv, Gang Zhao, Shunzi Jin
Long non-coding RNAs (lncRNAs) are a critical class of regulatory molecules involved in a variety of biological functions; however, their role in immune cells response to radiation is unknown. Therefore, in this study we used integrative analysis to determine the expression profile of lncRNAs in mouse thymocytes and the potential functions of lncRNAs in response to radiation. Microarray data profiling indicated that 53 lncRNAs (36 up-regulated and 17 down-regulated) and 74 coding genes (39 up-regulated and 35 down-regulated) were highly differentially expressed in the high dose radiation (HDR) group compared with the control group...
November 17, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27864277/molecular-characterization-of-ghpld%C3%AE-1-and-its-relationship-with-secondary-cell-wall-thickening-in-cotton-fibers
#9
Kai Tang, Jin-Yuan Liu
Phospholipase D (PLD) hydrolyzes phospholipids to generate a free polar head group (e.g., choline) and a second messenger phosphatidic acid and plays diverse roles in plant growth and development, including seed germination, leaf senescence, root hair growth, and hypocotyl elongation. However, the function of PLD in cotton remains largely unexplored. Here, the comprehensive molecular characterization of GhPLDα1 was explored with its role in upland cotton (Gossypium hirsutum) fiber development. The GhPLDα1 gene was cloned successfully, and a sequence alignment showed that GhPLDα1 contains one C2 domain and two HKD (HxKxxxxD) domains...
November 17, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27742678/mir-181a-5p-regulates-3t3-l1-cell-adipogenesis-by-targeting-smad7-and-tcf7l2
#10
Dan Ouyang, Lifeng Xu, Lihua Zhang, Dongguang Guo, Xiaotong Tan, Xiaofang Yu, Junjie Qi, Yaqiong Ye, Qihong Liu, Yongjiang Ma, Yugu Li
MicroRNAs are highly conserved non-coding small RNAs participating in almost all kinds of biological activities. MiR-181a has been reported to be involved in the differentiation of porcine primary preadipocytes, but the profound effect of miR-181a-5p on 3T3-L1 adipocyte differentiation and proliferation is still unclear. In this study, we found that supplementation of miR-181a-5p in 3T3-L1 cells significantly promoted the adipogenesis and inhibited cell proliferation with increased expression of adipogenic marker genes including peroxisome proliferator-activated receptor gamma (Pparγ), CCAAT/enhancer-binding protein alpha (C/ebpα), fatty acid-binding protein 4 (Fabp4), and Adiponectin, accompanied by an accumulation of lipid droplet, an increase of triglyceride content, and a decrease of cell proliferation...
October 14, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27733346/direct-interaction-between-mir-203-and-zeb2-suppresses-epithelial-mesenchymal-transition-signaling-and-reduces-lung-adenocarcinoma-chemoresistance
#11
Xunhuang Duan, Zhaojian Fu, Lingyuan Gao, Jin Zhou, Xiaojie Deng, Xiaojun Luo, Weiyi Fang, Rongcheng Luo
miR-203 is a tumor suppressor which participates in the pathogenesis of many tumors including lung adenocarcinoma. However, the role of miR-203 in suppressing chemotherapy resistance to cisplatin (cis-diamminedichloroplatinum; DDP) as well as its molecular mechanism is still to be determined in lung adenocarcinoma. In this study, we found that miR-203 decreased lung cancer cell migration and invasion, and that increased miR-203 expression sensitized lung adenocarcinoma cells to DDP in vitro Furthermore, ZEB2 was found to be a direct target of miR-203, which induces epithelial-mesenchymal transition (EMT) signal...
October 12, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27697751/mir-495-functions-as-an-adjuvant-to-radiation-therapy-by-reducing-the-radiation-induced-bystander-effect
#12
Jie Fu, Mengmeng Jiang, Meng Zhang, Jing Zhang, Yu Wang, Shensi Xiang, Xiaojie Xu, Qinong Ye, Haifeng Song
The radiation-induced bystander effect (RIBE) is an important factor in tumor radiation therapy because it may increase the probability of normal cellular injury and the likelihood of secondary cancers after radiotherapy. Here, we identified the role of miR-495 in alleviating RIBEs during radiotherapy. Luciferase reporter assay results confirmed that miR-495 regulated endothelial nitric oxide synthase (eNOS) by targeting the Sp1 3'-untranslated region. Consequently, after radiation, tumor cells expressed less eNOS and Sp1 than controls...
October 3, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27649892/energy-sources-identify-metabolic-phenotypes-in-pancreatic-cancer
#13
Chen Liang, Yi Qin, Bo Zhang, Shunrong Ji, Si Shi, Wenyan Xu, Jiang Liu, Jinfeng Xiang, Dingkong Liang, Qiangsheng Hu, Liang Liu, Chen Liu, Guopei Luo, Quanxing Ni, Jin Xu, Xianjun Yu
Metabolic reprogramming is one of the emerging hallmarks of cancers. As a highly malignant tumor, pancreatic ductal adenocarcinoma (PDA) is not only a metabolic disease but also a heterogeneous disease. Heterogeneity induces PDA dependence on distinct nutritive substrates, thereby inducing different metabolic phenotypes. We stratified PDA into four phenotypes with distinct types of energy metabolism, including a Warburg phenotype, a reverse Warburg phenotype, a glutaminolysis phenotype, and a lipid-dependent phenotype...
September 20, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27649891/low-levels-of-prss37-protein-in-sperm-are-associated-with-many-cases-of-unexplained-male-infertility
#14
Jianbing Liu, Chunling Shen, Weimin Fan, Yan Chen, Aijun Zhang, Yun Feng, Zheng Li, Ying Kuang, Zhugang Wang
PRSS37, a putative trypsin-like serine protease, is highly conserved during mammalian evolution as revealed by multiple sequence alignment. Mice deficient for Prss37 gene exhibit male infertility, but their mating behavior, spermatogenesis, sperm morphology, and motility remain unaffected, similar to a situation called unexplained male infertility (UMI) in men (human being). Here, we demonstrated that PRSS37 is restrictively expressed in human testis, where it is mainly located in the elongating and elongated spermatids during spermiogenesis as shown by immunohistochemical analysis of normal human testicular sections...
September 20, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27649890/ricin-b-lectin-enhances-microsporidia-nosema-bombycis-infection-in-bmn-cells-from-silkworm-bombyx-mori
#15
Han Liu, Mingqian Li, Shunfeng Cai, Xinyi He, Yongqi Shao, Xingmeng Lu
Nosema bombycis is an obligate intracellular parasitic fungus that utilizes a distinctive mechanism to infect Bombyx mori Spore germination can be used for host cell invasion; however, the detailed mechanism remains to be elucidated. The ricin-B-lectin (RBL) gene is significantly differentially regulated after N. bombycis spore germination, and NbRBL might play roles in spore germination and infection. In this study, the biological function of NbRBL was examined. Protein sequence analysis showed that NbRBL is a secreted protein that attaches to carbohydrates...
September 20, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27624953/ascl1-represses-the-mesendoderm-induction-in-xenopus
#16
Zheying Min, Hao Lin, Xuechen Zhu, Li Gao, Aftab A Khand, Qinghua Tao
Ascl1 is a multi-functional regulator of neural development in invertebrates and vertebrates. Ectopic expression of Ascl1 can generate functional neurons from non-neural somatic cells. The abnormal expression of ASCL1 has been reported in several types of carcinomas. We have previously identified Ascl1 as a crucial maternal regulator of the germ layer pattern formation in Xenopus Functional studies have indicated that the maternally-supplied Ascl1 renders embryonic cells a propensity to adopt neural fates on one hand, and represses the mesendoderm formation on the other...
September 13, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27827798/unimpaired-immunogenicity-of-yeast-expressed-hepatitis-b-surface-antigen-stored-at-elevated-temperatures
#17
Zhongliang Shen, Yuliang Rao, Shuai Tao, Mengjun Luo, Lijun Ming, Jing Liu, Shaokun Pan, Youhua Xie
Global adoption of hepatitis B virus (HBV) vaccines has greatly reduced new HBV infections. Current HBV vaccines are liquid suspensions containing recombinant hepatitis B surface antigen (HBsAg) particles mixed with aluminum phosphate or aluminum hydroxide. Refrigeration (2-8°C) as recommended for vaccine transport and storage may be unachievable in certain HBV-prevalent developing countries or regions. In this study, we stored yeast-expressed HBsAg and aluminum hydroxide separately at the standard (4°C) and elevated temperature (25, 37, or 45°C) for 14, 23, and 30 days, then mixed them and used the mixture to vaccinate mice with a prime-boost program...
December 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27827797/the-clustered-regularly-interspaced-short-palindromic-repeats-associated-proteins-system-for-the-induction-of-gene-mutations-and-phenotypic-changes-in-bombyx-mori
#18
Jia Song, Jiaqian Che, Zhengying You, Xiaogang Ye, Jisheng Li, Lupeng Ye, Yuyu Zhang, Qiujie Qian, Boxiong Zhong
To probe the general phenomena of gene mutations, Bombyx mori, the lepidopterous model organism, was chosen as the experimental model. To easily detect phenotypic variations, the piggyBac system was utilized to introduce two marker genes into the silkworm, and 23.4% transposition efficiency aided in easily breeding a new strain for the entire experiment. Then, the clustered regularly interspaced short palindromic repeats/an associated protein (Cas9) system was utilized. The results showed that the Cas9 system can induce efficient gene mutations and the base changes could be detected since the G0 individuals in B...
December 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27797722/antitumor-effect-of-dobutamine-on-multiple-myeloma-via-mitogen-activated-protein-kinase%C3%A2-pathway-in-vitro
#19
Bingqian Xie, Zhijian Xu, Guang Yang, Gege Chen, Bo Li, Liangning Hu, Wenqin Xiao, Xi Sun, Minjie Gao, Lu Gao, Xiaosong Wu, Yi Tao, Weiliang Zhu, Jumei Shi
No abstract text is available yet for this article.
December 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27797721/zfx-modulates-the-growth-of-human-leukemic-cells-via-b4galt1
#20
Jie Wu, Lun Xiao, Haixia Zhou, Hong Liu, Yue Ge, Jing Yang, Yuanyuan Li, Depei Wu, Yun Zhao, Xiuyan Zhang
Zinc finger protein X-linked (ZFX) is a key regulator of both embryonic stem cells (ESCs) and hematopoietic stem cells (HSCs), which is required for both Notch intracellular domain (NotchIC)-induced acute T-cell leukemia and MLL-AF9-induced myeloid leukemia in mouse models. However, the role of ZFX and its underlying mechanism in human leukemic cells remain unclear yet, though accumulating data have demonstrated that ZFX is aberrantly expressed in various human tumors and plays an important role. Herein, we found that ZFX was aberrantly expressed in various human leukemic cell lines and primary cells from leukemia patients compared with control cells...
December 2016: Acta Biochimica et Biophysica Sinica
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