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Acta Biochimica et Biophysica Sinica

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https://www.readbyqxmd.com/read/29140406/smad3-stat3-crosstalk-in-pathophysiological-contexts
#1
Yuka Itoh, Masao Saitoh, Keiji Miyazawa
Smad3 and STAT3 are intracellular molecules that transmit signals from plasma membrane receptors to the nucleus. Smad3 operates downstream of growth/differentiation factors that utilize activin receptor-like kinase (ALK)-4, 5, or 7, such as transforming growth factor-β (TGF-β), activin, and myostatin. STAT3 principally functions downstream of cytokines that exert their effects via gp130 and Janus family kinases, including interleukin-6 (IL-6), leukemia inhibitory factor (LIF), and oncostatin M. Accumulating evidence indicates that Smad3 and STAT3 engage in crosstalk in a highly context-dependent fashion, cooperating in some conditions while acting antagonistically each other in others...
November 13, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29121225/time-dependent-glucocorticoid-administration-differently-affects-peripheral-circadian-rhythm-in-rats
#2
Tao Wu, Zhengwei Fu
There is a growing recognition that glucocorticoid (GC) acts as an internal timing signal for peripheral circadian oscillators. However, the transcription process of GC-related clock gene in the peripheral tissues is not fully understood. The present study was designed to explore the potential role of clock genes in the GC-induced peripheral circadian gene expression invivo. Real-time RT-PCR analysis indicated that the transcript levels of Per1 and Dec1 were rapidly up-regulated within 0.5 and 1 h in the heart and kidney respectively after stimulation with dexamethasone (Dex)...
November 7, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29121163/gene-expression-profiling-reveals-heterogeneity-of-perivascular-adipose-tissues-surrounding-coronary-and-internal-thoracic-arteries
#3
Danbo Lu, Wei Wang, Limin Xia, Pu Xia, Yan Yan
The internal thoracic artery (ITA) that differs from coronary artery (CA), rarely develops atherosclerosis. Understanding the mechanism underlying such a difference will help to pave a new way to the prevention and treatment of the disease. We hypothesize herein that the difference in susceptibility to atherosclerosis between CA and ITA is attributable to the heterogeneity of perivascular adipose tissues (PVATs) surrounding these two kinds of arteries, i.e. PVAT-CA and PVAT-ITA. We isolated PVAT from eight patients of coronary heart disease (CHD) and four non-CHD patients...
November 7, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29121159/structure-and-function-analysis-of-polygonatum-cyrtonema-lectin-by-site-directed-mutagenesis
#4
Yuyu Chen, Kaimin Lu, Jianzong Li, Danfeng Liang, Hao Luo, Xiaoyun Wang, Xin Wang, Jinku Bao
The crystal structure of mature Polygonatum cyrtonema lectin (PCL) showed three similar carbohydrate-binding sites (CBS I, CBS II, and CBS III). The Gln58 and Asp60 residues of CBS II are substituted with His58 and Asn60. To establish the relationship between the key amino acid residues and structure or activity of PCL, we constructed four recombinant mutants in CBS I, CBS II, and CBS III. The experimental results indicate that CBS I, CBS III and the disulfide bond play vital roles in the binding with mannose...
November 7, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29095973/antimicrobial-activity-and-mechanism-of-larch-bark-procyanidins-against-staphylococcus-aureus
#5
Xinchao Li, Congfen He, Liya Song, Ting Li, Shumei Cui, Liping Zhang, Yan Jia
Larch bark procyanidins (LBPC) have not only antioxidant and antitumor properties, but also strong bacteriostatic effects. However, it is not clear about the antibacterial mechanisms of LBPC. In this work, the antibacterial effects and mechanisms of LBPC on Staphylococcus aureus were studied in the aspects of morphological structure, cell wall and membrane, essential proteins, and genetic material. The results showed that LBPC effectively inhibited bacterial growth at a minimum inhibitory concentration of 1...
October 31, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29087452/tar-cloning-and-integrated-overexpression-of-6-demethylchlortetracycline-biosynthetic-gene-cluster-in-streptomyces-aureofaciens
#6
Naxin Wu, He Huang, Taoling Min, Haifeng Hu
6-Demethylchlortetracycline (6-DCT), a tetracycline antibiotic produced by Streptomyces aureofaciens, is a crucial precursor employed for the semi-synthesis of tigecycline, minocycline, and amadacyclin (PTK 0796). In this study, the 6-DCT biosynthetic gene cluster (BGC) was cloned from genomic DNA of a high 6-DCT-producing strain, S. aureofaciens DM-1, using the transformation-associated recombination method. An extra copy of the 6-DCT BGC was introduced and integrated into the chromosome of S. aureofaciens DM-1...
October 26, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29077787/mir-219a-5p-inhibits-breast-cancer-cell-migration-and-epithelial-mesenchymal-transition-by-targeting-myocardin-related-transcription-factor-a
#7
Chunyu Zhuang, Ying Yuan, Tiefeng Song, Huiqin Wang, Liwen Huang, Xuegang Luo, Hongpeng He, Lihong Huo, Hao Zhou, Nan Wang, Tongcun Zhang
Although many miRNAs are reported to be involved in tumor formation and progression, the effect of miR-219a-5p on breast cancer metastasis is not well-known. The aim of this study is to investigate the effect of miR-219a-5p on the migratory ability and epithelial-mesenchymal transition (EMT) of breast cancer cells. First, miR-219a-5p was found to be highly expressed in low-invasive breast cancer MCF-7 cells, but lowly expressed in high-invasive breast cancer MDA-MB-231 cells. Wound scratch assay and transwell assay showed that miR-219a-5p inhibited the migratory ability of MDA-MB-231 cells...
October 25, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29077784/effects-of-high-intensity-focused-ultrasound-on-cisplatin-resistant-human-lung-adenocarcinoma-in-vitro-and-in-vivo
#8
Tao Zhang, Libin Chen, Shengmin Zhang, Youfeng Xu, Yabo Fan, Lizhong Zhang
It is widely accepted that high-intensity focused ultrasound (HIFU) is a minimally invasive treatment option for different tumors, but its roles and the corresponding mechanism in cisplatin (DDP) chemoresistance in lung adenocarcinoma (LA) remain unclear. In this study, we investigated the response of DDP-resistant LA cells to HIFU and its underlying molecular mechanisms using molecular biology techniques. It was found that HIFU exposure inhibited the proliferation of DDP-resistant A549 (A549/DDP) cells through arresting cell cycle at the G1/G0 phase via the Cyclin-dependent pathway and promoting apoptosis in a Bcl-2-dependent manner...
October 25, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29069287/the-concomitant-apoptosis-and-emt-underlie-the-fundamental-functions-of-tgf-%C3%AE
#9
Jianguo Song, Weiwei Shi
TGF-β's multipotent cellular effects and their relations are critical for TGF-β's pathophysiological functions. However, these effects may appear to be paradoxical in understanding TGF-β's functions. Apoptosis and epithelial-mesenchymal transition (EMT) are two fundamental events that are deeply linked to various physiological and disease-related processes. These two major cellular fates are subtly regulated and can be potently stimulated by TGF-β, which profoundly contribute to the biological roles of TGF-β...
October 21, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29040365/role-of-the-akt-mtor-signaling-pathway-in-human-papillomavirus-associated-nasal-and-sinonasal-inverted-papilloma
#10
Yongliang Liu, Lihua Duan, Jie Tian, Daoliang Song, Min Zhang, Shenlin Zhao, Zhaofu Yin, Xinxin Xiang, Xuezhong Li
Nasal and sinonasal inverted papilloma (NSIP) is a benign tumor in which surface epithelial cells grow downward into the underlying supportive tissue with varying degrees of metaplasia. Human papillomavirus (HPV) has been proposed as the causal agent in the pathogenesis of this disease. Many studies have shown that HPV can activate the Akt/mechanistic target of rapamycin (mTOR) signaling pathway, but the role of this pathway in HPV-associated NSIP is largely unknown. In this study, we enrolled 40 control tissue samples and 80 NSIP tissue samples...
October 13, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29040392/%C3%AE-cypermethrin-and-its-metabolite-3-phenoxybenzoic-acid-exhibit-immunotoxicity-in-murine-macrophages
#11
Xia Wang, Bingnan He, Baida Kong, Lai Wei, Rong Wang, Chenqian Zhou, Yiyan Shao, Jiajia Lin, Yuanxiang Jin, Zhengwei Fu
β-Cypermethrin (β-CYP), one of most important pyrethroids, is widely used to control insects, and has been detected in organisms, including human. Pyrethroids have been shown to pose neurotoxicity, hepatotoxicity, endocrine disruption and reproductive risks in mammals. However, research in immunotoxicity of pyrethroids, especially their metabolites, is limited. A common metabolite of pyrethroids is 3-phenoxybenzoic acid (3-PBA) in mammals. Thus, in this study, we evaluated the immunotoxicity of β-CYP and 3-PBA in mouse macrophages, RAW 264...
October 11, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29040348/desumoylation-signaling-a-novel-mechanism-of-liver-csc-properties-and-hepatocarcinogenesis-in-hypoxia
#12
Hong Zhao, Pingbo Yao, Nian Fu, Linxi Chen
No abstract text is available yet for this article.
October 11, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29036539/immune-cells-and-autoantibodies-in-pulmonary-arterial-hypertension
#13
Cheng Li, Pingping Liu, Rong Song, Yiqing Zhang, S Lei, Shangjie Wu
Analyses of immunity in pulmonary arterial hypertension (PAH) support the notion that maladaptation of the immune response exists. Altered immunity is an increasingly recognized feature of PAH. Indeed, a delicate balance between immunity and tolerance exists and any disturbance may result in chronic inflammation or autoimmunity. This is suggested by infiltration of various immune cells (e.g. macrophages, T and B lymphocytes) in remodeled pulmonary vessels. In addition, several types of autoantibodies directed against antinuclear antigens, endothelial cells (ECs) and fibroblasts have been found in idiopathic and systemic sclerosis-associated PAH...
September 27, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29036564/elabela-apj-axis-a-novel-therapy-target-for-cardiovascular-diseases
#14
Lanfang Li, Qionglin Zhou, Xiaoxiao Li, Linxi Chen
No abstract text is available yet for this article.
November 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29036538/stlr4-md-2-complex-inhibits-colorectal-cancer-migration-and-invasiveness-in-vitro-and-in-vivo-by-lncrna-h19-down-regulation
#15
Weijun Liang, Yan Zou, Fengxian Qin, Jifei Chen, Junyi Xu, Shifeng Huang, Jingfan Chen, Shengming Dai
Long non-coding RNAs (lncRNAs) have multiple functions in gene regulation and during cellular processes. However, the functional roles of lncRNAs in colorectal cancer (CRC) have not yet been well understood. In our previous study, we demonstrated that sTLR4/MD-2 complex can inhibit CRC in vitro and in vivo by targeting LPS. Therefore, the aim of the present study is to investigate the expression of lncRNA H19 in CRC and to evaluate its effect on the inhibition of sTLR4/MD-2 complex. The expression of H19 is measured in 63 CRC tumor tissues and adjacent normal tissues by quantitative real-time PCR (qRT-PCR)...
November 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29036495/the-p16-mir-217-egr1-pathway-modulates-age-related-tenogenic-differentiation-in-tendon-stem-progenitor-cells
#16
Weifeng Han, Bing Wang, Junpeng Liu, Lei Chen
Previous studies have shown that the differentiation potential declines with the age of progenitor cells and is linked to altered levels of senescence markers. The purpose of this study was to test whether senescence marker p16 affects age-related tenogenic differentiation in tendon stem/progenitor cells (TSPCs). Young and aged TSPCs were isolated from young/healthy and aged/degenerated human Achilles tendons, respectively. Cellular aging and capacity for tenogenic differentiation were examined. The results showed that the tenogenic differentiation capacity of TSPCs significantly decreases with advancing age...
November 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29036449/resveratrol-attenuates-bone-cancer-pain-through-regulating-the-expression-levels-of-asic3-and-activating-cell-autophagy
#17
Haili Zhu, Jieqiong Ding, Ji Wu, Tingting Liu, Jing Liang, Qiong Tang, Ming Jiao
Bone cancer pain (BCP) is one of the most common pains in patients with malignant cancers. The mechanism underlying BCP is largely unknown. Our previous studies and the increasing evidence both have shown that acid-sensing ion channels 3 (ASIC3) is an important protein in the pathological pain state in some pain models. We hypothesized that the expression change of ASIC3 might be one of the factors related to BCP. In this study, we established the BCP model through intrathecally injecting rat mammary gland carcinoma cells (MRMT-1) into the left tibia of Sprague-Dawley female rats, and found that the BCP rats showed bone destruction, increased mechanical pain sensitivities and up-regulated ASIC3 protein expression levels in L4-L6 dorsal root ganglion...
November 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29036390/endothelial-cells-modified-by-adenovirus-vector-containing-nine-copies-hypoxia-response-elements-and-human-vascular-endothelial-growth-factor-as-the-novel-seed-cells-for-bone-tissue-engineering
#18
Xiaobin Song, Liang Shi, Lamei Chen, Xinyu Liu, Xun Qu, Ketao Wang, Fengcai Wei
Vascularization is one of the hotspots during the development of new therapeutic strategies for bone tissue engineering, which can alleviate hypoxic circumstance and prevent transplant failure. Vascular endothelial growth factor (VEGF) gene transfection using recombinant adenovirus (Ad) vector can effectively promote angiogenesis, but uncontrolled long-term continuous expression of VEGF brings safety concern. Here we constructed a recombinant Ad vector containing nine copies of HRE promoter and the hVEGF165 gene, which conserved the oxygen sensitivity of hypoxia-inducible factor-1/hypoxia response elements (HIF-1/HRE)...
November 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29036356/lipopolysaccharide-promotes-angiogenesis-in-mice-model-of-hcc-by-stimulating-hepatic-stellate-cell-activation-via-tlr4-pathway
#19
Yi Lu, Jianliang Xu, Shuxian Chen, Zheng Zhou, Nan Lin
Angiogenesis plays a key role in the progression of hepatocellular carcinoma (HCC). This study aimed to investigate whether lipopolysaccharide (LPS) could promote HCC angiogenesis and the role of hepatic stellate cell (HSC) in this process. In vivo orthotopic HCC model and the effect of LPS on HSC in vitro were studied. Our results demonstrated that LPS-induced HSC activation during the promotion of HCC growth and angiogenesis in mice. The LPS-TLR4 (Toll-like receptor 4) pathway in HSC is responsible for HCC angiogenesis...
November 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29036266/alterations-in-no-ros-ratio-and-expression-of-trx1-and-prdx2-in-isoproterenol-induced-cardiac-hypertrophy
#20
Hao Su, Marco Pistolozzi, Xingjuan Shi, Xiaoou Sun, Wen Tan
The development of cardiac hypertrophy is a complicated process, which undergoes a transition from compensatory hypertrophy to heart failure, and the identification of new biomarkers and targets for this disease is greatly needed. Here we investigated the development of isoproterenol (ISO)-induced cardiac hypertrophy in an in vitro experimental model. After the induction of hypertrophy with ISO treatment in H9c2 cells, cell surface area, cell viability, cellular reactive oxygen species (ROS), and nitric oxide (NO) levels were tested...
November 1, 2017: Acta Biochimica et Biophysica Sinica
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