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Acta Biochimica et Biophysica Sinica

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https://www.readbyqxmd.com/read/28104582/amniotic-fluid-derived-stem-cells-mixed-with-platelet-rich-plasma-for-restoration-of-rat-alveolar-bone-defect
#1
Minjiao Wang, Hongliang Li, Jiawen Si, Jiewen Dai, Jun Shi, Xudong Wang, Lihe Guo, Guofang Shen
Stem cells isolated from the amniotic fluid have been shown as a promising candidate for cell therapy and tissue engineering. However, the experimental and preclinical applications of amniotic fluid-derived stem cells (AFSCs) in the very field of maxillofacial bone tissue engineering are still limited. In this study, rat AFSCs were successfully harvested and characterized in vitro The rat AFSCs showed typical fibroblastoid morphology, stable proliferation activity and multi-differentiation potential. Flow-cytometry analysis demonstrated that these cells were positive for CD29, CD44, and CD90, while negative for hematopoietic markers such as CD34 and CD45...
January 18, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28104581/high-glucose-stimulates-proliferation-and-inhibits-apoptosis-of-non-small-cell-lung-cancer-cells-by-jnk-mediated-downregulation-of-p53-pathway
#2
Lumei Wang, Nanzhe Zhong, Shujuan Liu, Xiaoyan Zhu, Yujian Liu
No abstract text is available yet for this article.
January 18, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28069586/sqle-induces-epithelial-to-mesenchymal-transition-by-regulating-of-mir-133b-in-esophageal-squamous-cell-carcinoma
#3
Yi Qin, Yi Zhang, Qinting Tang, Li Jin, Yong'an Chen
Increasing evidence suggests that microRNAs, which control gene expression at the post-transcriptional level, are aberrantly expressed in cancers and play significant roles in carcinogenesis and cancer progression. In this study, we show differential miR-133b down-expression in human esophageal squamous cell carcinoma (ESCC) cells and tissues. In addition, squalene epoxidase (SQLE), a key enzyme of cholesterol synthesis, is identified as the direct downstream target gene of miR-133b by luciferase gene reporter assay...
January 9, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28069585/expression-of-hmgb2-indicates-worse-survival-of-patients-and-is-required-for-the-maintenance-of-warburg-effect-in-pancreatic-cancer
#4
Xin Cai, Hongjian Ding, Yanxia Liu, Gaofeng Pan, Qingguo Li, Zhen Yang, Weiyan Liu
High mobility group proteins (HMGs) are the second most abundant chromatin proteins and exert global genomic functions in the establishment of active or inactive chromatin domains. Through interaction with nucleosomes, transcription factors, nucleosome-remodeling machines and histones, the HMGs family proteins contribute to the fine tuning of transcription in response to rapid environmental changes. Mammalian high mobility group Bs (HMGBs) are characterized by two tandem HMG box domains followed by a long acidic tail...
January 9, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28069584/role-of-norepinephrine-in-a%C3%AE-related-neurotoxicity-dual-interactions-with-tyr10-and-snk-26-28-of-a%C3%AE
#5
Mengmeng Liu, Liping Wan, Yannan Bin, Juan Xiang
With their capability to inhibit the formation of amyloid-β peptide (Aβ) fibril, norepinephrine (NE), and other catechol derivatives have been considered for the potential treatment of Alzheimer's disease (AD). Such treatment, however, remains debatable because of the diverse functions of Aβ and NE in AD pathology. Moreover, the complicated oxidation accompanying NE has caused the majority of the previous research to focus on the binding of NE oxides onto Aβ. The molecular mechanism by which Aβ interacts with the reduction state of NE, which is correlated with the brain function, should be urgently explored...
January 8, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28069583/mir-375-suppresses-invasion-and-metastasis-by-direct-targeting-of-shox2-in-esophageal-squamous-cell-carcinoma
#6
Jun Yi, Li Jin, Jing Chen, Bing Feng, Zhenyue He, Longbang Chen, Haizhu Song
Esophageal squamous cell carcinoma (ESCC) is the most common histological type in China. MicroRNAs are endogenously expressed in mammals and play a significant role in tumor invasion and metastasis by targeting potential downstream genes. In the present study, microarray analysis showed that miR-375 expression was distinctly downregulated in ESCC compared with that in normal esophageal epithelium tissues. Then, luciferase reporter assay showed that SHOX2 was the direct downstream target of miR-375 and this interaction was confirmed by the rescue experiments...
January 8, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28069582/human-apolipoprotein-a-i-exerts-a-prophylactic-effect-on-high-fat-diet-induced-atherosclerosis-via-inflammation-inhibition-in-a-rabbit-model
#7
Jiyang Li, Weina Wang, Lei Han, Meiqing Feng, Hui Lu, Li Yang, Xiangxiang Hu, Si Shi, Shanshan Jiang, Qian Wang, Li Ye
Apolipoprotein A-I (apoA-I) is the major functional protein fraction of high-density lipoprotein. The prophylactic effect and mechanism of human apoA-I on atherosclerosis (AS) were investigated in a high-fat diet-induced AS rabbit model. The rabbits were injected with apoA-I once a week while fed high-fat diet for 20 weeks. Our results showed that apoA-I could raise the serum level of high-density lipoprotein-cholesterol and reduce those of lipid total cholesterol, triglyceride, and low-density lipoprotein-cholesterol in AS rabbits...
January 8, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28040679/morphological-observation-rna-seq-quantification-and-expression-profiling-novel-insight-into-grafting-responsive-carotenoid-biosynthesis-in-watermelon-grafted-onto-pumpkin-rootstock
#8
Guang Liu, Xingping Yang, Jinhua Xu, Man Zhang, Qian Hou, Lingli Zhu, Ying Huang, Aisheng Xiong
Watermelon is an important and economical horticultural crop in China, where ~20% of the plants are grafted. The development of grafted watermelon fruit involves a diverse range of gene interactions that results in dynamic changes in fruit. However, the molecular mechanisms underlying grafting-induced fruit quality change are unclear. In the present study, we measured the lycopene content by high-performance liquid chromatography and used RNA-Seq (quantification) to perform a genome-wide transcript analysis of fruits from watermelon grafted onto pumpkin rootstock (pumpkin-grafted watermelon, PGW), self-grafted watermelon (SGW), and non-grafted watermelon (NGW)...
December 30, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28039150/the-effects-and-regulatory-mechanism-of-rip3-on-rgc-5-necroptosis-following-elevated-hydrostatic-pressure
#9
Lei Shang, Wei Ding, Na Li, Lvshuang Liao, Dan Chen, Jufang Huang, Kun Xiong
Necroptosis is a type of regulated cell death that has been implicated in various diseases. Receptor-interacting protein 3 (RIP3), a member of the RIP family, is an important mediator of the necroptotic pathway. Cleavage of RIP3 at Asp328 by caspase-8 abolishes the kinase activity of RIP3, which is critical for necroptosis. Moreover, RIP3 is significantly upregulated during the early stages of acute high intra-ocular pressure and oxygen glucose deprivation. In this study, the effects of RIP3 during elevated hydrostatic pressure (EHP) were investigated and the possible mechanism through which caspase-8 regulated RIP3 cleavage was explored...
December 29, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28039149/lysine-acetylation-regulates-the-activity-of-escherichia-coli-pyridoxine-5-phosphate-oxidase
#10
Jing Gu, Yuanyuan Chen, Hongsen Guo, Manluan Sun, Mingkun Yang, Xude Wang, Xian'en Zhang, Jiaoyu Deng
Nɛ-lysine acetylation is one of the most abundant post-translational modifications in eukaryote and prokaryote. Protein acetylome of Escherichia coli has been screened using mass spectrometry (MS) technology, and many acetylated proteins have been identified, including the pyridoxine 5'-phosphate oxidase (EcPNPOx), but the biological roles played by lysine acetylation in EcPNPOx still remain unknown. In this study, EcPNPOx was firstly overexpressed and purified, and two acetylated lysine residues were identified by the subsequent liquid chromatography-tandem mass spectrometry analysis...
December 29, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28039148/gpx1-knockdown-suppresses-chondrogenic-differentiation-of-atdc5-cells-through-induction-of-reductive-stress
#11
Jidong Yan, Yuanxu Guo, Yao Fei, Rui Zhang, Yan Han, Shemin Lu
Glutathione peroxidase 1 (GPx1) is a selenium (Se)-containing protein and is induced in cartilage formation. GPx1 eliminates reactive oxygen species (ROS), which are required for chondrogenic induction. The physiological properties of GPx1 in cartilage and the redox mechanisms involved are not known. The effects of GPx1 on chondrogenic differentiation of ATDC5 cells were examined through short hairpin RNA-mediated gene silencing. The results demonstrated that GPx1 knockdown impaired gene expression of sex determining region Y-box 9, collagen II (Col II), and aggrecan...
December 29, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28039147/incorporation-of-nlr-into-nih-stratification-system-increases-predictive-accuracy-for-surgically-resected-gastrointestinal-stromal-tumors
#12
Anwei Xue, Xiaodong Gao, Yong Fang, Ping Shu, Jiaqian Ling, Kuntang Shen, Yihong Sun, Jing Qin, Xinyu Qin, Yingyong Hou
Previous studies have reported that preoperative neutrophil-to-lymphocyte (NLR) and platelet-to-lymphocyte ratios were prognostic for various types of cancers. The aim of this study was to investigate the predictive utilities of preoperative peripheral blood counts in patients with gastrointestinal stromal tumors (GISTs). We enrolled 510 consecutive, previously untreated patients who underwent surgery for primary, localized GISTs. The relationship between recurrence-free survival and outcome variables was assessed by univariate and multivariate analyses, while the clinicopathologic relevance of NLR was determined using the Chi-square test...
December 29, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28028056/regucalcin-plays-a-role-in-the-cytoskeleton-regulation-of-hepg2-cells
#13
Jia Chen, Beihui Xu, Jiemin Wu, Xiangfan Liu, Hong Xu, Peihua Ni
No abstract text is available yet for this article.
December 26, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27989993/isolation-and-identification-of-stem-cells-from-degenerated-human-intervertebral-discs-and-their-migration-characteristics
#14
Shuhao Liu, Haifeng Liang, Soo-Min Lee, Zheng Li, Jian Zhang, Qinming Fei
Mesenchymal stem cells (MSCs) have been isolated and identified separately from the three components of intervertebral disc, i.e. annulus fibrosus (AF), nucleus pulposus (NP), and cartilage endplate (CEP). However, few studies have been carried out to compare the properties of these three kinds of stem cells, especially their migration ability which is essential for their potential clinical application. In this study, MSCs were isolated from AF, NP, and CEP, respectively, of human degenerated discs and identified by surface markers and multilineage differentiation assay at passage 3...
December 18, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27974288/virus-like-particle-vaccine-by-intranasal-vaccination-elicits-protective-immunity-against-respiratory-syncytial-viral-infection-in-mice
#15
Mengying Cai, Cheng Wang, Yufeng Li, Hongjing Gu, Sujing Sun, Yueqiang Duan, Chengcai Lai, Keyu Wang, Xiaolan Yang, Li Xing, Peirui Zhang, Zhaohai Wang, Shaogeng Zhang, Xiaodong Guo, Shubing Liu, Yigang Tong, Xiliang Wang, Penghui Yang
Respiratory syncytial virus (RSV) is a leading cause of lower respiratory infection in infants and children, but there is still no licensed vaccine available. In this report, we developed virus-like particle (VLP) vaccines based on the Bac-to-Bac baculovirus expression system, consisting of an influenza virus matrix (M1) protein and the RSV fusion protein (F) or glycoprotein (G). These RSV VLPs were identified by western blot analysis and electron microscopy. Female BALB/c mice immunized intranasally (i.n.) with RSV-F VLPs, RSV-G VLPs, or both showed viral-specific antibody responses against RSV...
January 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27932393/biological-characteristics-of-embryonic-epicardial-cells-in-vitro-correlate-with-embryonic-day
#16
Xiaoming Wei, Yulin Gao, Xiaodong Jing, Songbai Deng, Jianlin Du, Yajie Liu, Qiang She
The epicardial cell (EpiC) culture system plays an important role in investigating the specific mechanisms and signaling molecules that are involved in the development of EpiCs. From this early formation until adulthood, EpiCs undergo dynamic changes in the expression of embryonic genes that correlate with changes in the embryonic EpiC properties. The differences of embryonic EpiC properties may affect the related results of experiments in which EpiC culture system is used; however, these differences have not been explored...
January 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27932392/digital-gene-expression-profiling-analysis-of-dna-repair-pathways-in-colon-cancer-stem-population-of-ht29-cells
#17
Wenxue Wang, Guoxiu Zhang, Jing Yang, Huan Gu, Lei Ding, Haijing Yu, Min Yu, Qinghua Cui, Xinglai Ji, Meizhang Li
Cancer stem cells (CSCs) contribute to the relapse and development of new neoplasm lesions. While most available clinical approaches, such as chemical and radiation therapies, will kill the majority of cancer cells, they do not kill them all. Some resisting cells, like CSCs, are able to survive due to their excellent self-maintaining capabilities, even in challenging environments. In the present study, we investigated the mRNA level of DNA repair genes of colon CSCs from the HT29 cell line in response to single-strand damage and double-strand breaks, as well as the evident upregulation of key genes in base excision repair, mismatch repair, non-homologous end-joining, and homologous recombination pathways in these cells...
January 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27864283/tumor-necrosis-factor-%C3%AE-a-key-contributor-to-intervertebral-disc-degeneration
#18
REVIEW
Cheng Wang, Xiaohua Yu, Yiguo Yan, Wei Yang, Shujun Zhang, Yongxiao Xiang, Jian Zhang, Wenjun Wang
Intervertebral disc (IVD) degeneration (IDD) is the most common cause leading to low back pain (LBP), which is a highly prevalent, costly, and crippling condition worldwide. Current treatments for IDD are limited to treat the symptoms and do not target the pathophysiology. Tumor necrosis factor-α (TNF-α) is one of the most potent pro-inflammatory cytokines and signals through its receptors TNFR1 and TNFR2. TNF-α is highly expressed in degenerative IVD tissues, and it is deeply involved in multiple pathological processes of disc degeneration, including matrix destruction, inflammatory responses, apoptosis, autophagy, and cell proliferation...
January 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27864282/bxb1-integrase-serves-as-a-highly-efficient-dna-recombinase-in-rapid-metabolite-pathway-assembly
#19
Xianwei Wang, Biao Tang, Yu Ye, Yayi Mao, Xiaolai Lei, Guoping Zhao, Xiaoming Ding
Phage-encoded serine integrases are widely used in genetic engineering. They also have the potential to serve as efficient DNA assemblers, demonstrated by the method of site-specific recombination-based tandem assembly (SSRTA) that can combine biological parts into devices, pathways, and systems. Here, four serine integrases, ϕBT1, TG1, ϕRv1, and Bxb1, were investigated to ascertain their in vitro DNA assembly activities. Bxb1 integrase displayed the highest efficiency to obtain final products. Thus, we conclude that Bxb1 integrase is an excellent choice for DNA assembly in vitro Using this enzyme and its recognition sites, BioBrick standards were designed that are compatible with the SSRTA method for module addition...
January 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27864281/lipoprotein-lipase-and-phospholipid-transfer-protein-overexpression-in-human-glioma-cells-and-their-effect-on-cell-growth-apoptosis-and-migration
#20
Weijiang Dong, Huilin Gong, Guanjun Zhang, Simona Vuletic, John Albers, Jiaojiao Zhang, Hua Liang, Yanxia Sui, Jin Zheng
Glioma is one of the common tumors in brain. The expression level of lipoprotein lipase (LPL) or phospholipid transfer protein (PLTP) may influence glioma progression and its relationship with clinical and pathological parameters. The clinical significance of LPL or PLTP expression in glioma has not been established. In the present study, the LPL and PLTP levels in glioma tumors were investigated and the relationship between the LPL and PLTP level and the grade of malignant glioma was analyzed, with the aim to provide new ideas for the diagnosis and treatment of gliomas in clinical and basic research settings...
January 2017: Acta Biochimica et Biophysica Sinica
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