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Acta Biochimica et Biophysica Sinica

Yuhui Zhang, Yixuan Zhang, Peng Li, Ying Wang, Jin Wang, Zhihui Shao, Guoping Zhao
Amycolatopsis mediterranei U32 is an important industrial strain for the production of rifamycin SV. Rifampicin, a derivative of rifamycin SV, is commonly used to treat mycobacterial infections. Although phosphate has long been known to affect rifamycin biosynthesis, phosphate transport, metabolism, and regulation are poorly understood in A. mediterranei. In this study, the functional phosphate transport system pstSCAB was isolated by RNA sequencing and inactivated by insertion mutation in A. mediterranei U32...
July 11, 2018: Acta Biochimica et Biophysica Sinica
Dandan Yu, Yong Zhang, Gege Chen, Yongsheng Xie, Zhijian Xu, Shuaikang Chang, Liangning Hu, Bo Li, Wenxuan Bu, Yingcong Wang, Wenqin Xiao, Xi Sun, Gaomei Chang, Lu Gao, Sujing Qiang, Xiaosong Wu, Weiliang Zhu, Jumei Shi
Mantle cell lymphoma (MCL) is an aggressive and mostly incurable B-cell malignancy with frequent relapses after an initial response to standard chemotherapy. Therefore, novel therapies are urgently required to improve MCL clinical outcomes. In this study, MCL cell lines were treated with pterostilbene (PTE), a non-toxic natural phenolic compound primarily found in blueberries. The antitumor activity of PTE was examined by using the Cell Counting Kit-8, apoptosis assays, cell cycle analysis, JC-1 mitochondrial membrane potential assay, western blot analysis, and tumor xenograft models...
June 29, 2018: Acta Biochimica et Biophysica Sinica
Ying Xu, Xiaojia Wang, Jing Liu, Yong Fu, Jianhai Xu, Qun Liu
Toxoplasma rhoptry protein 38 (TgROP38) is a new active kinase that modulates host cell signal transduction and TgROP38 expression shows strain-specificity and stage-specificity in different isolates. In the present study, we overexpressed ROP38 in the RH and prugniaud (PRU) strain (RH+rop38II and PRU+rop38II), disrupted ROP38 (PRUΔROP38) in the PRU strain, complemented the ROP38 (PRUΔROP38comp+) in the PRUΔROP38 strain, and compared phenotypes of gene-edited and parental strains. We found that knockout of ROP38 led to increased proliferation (P < 0...
June 29, 2018: Acta Biochimica et Biophysica Sinica
Qiang Zhang, Bin Zhang, Leina Sun, Qingna Yan, Yu Zhang, Zhenfa Zhang, Yanjun Su, Changli Wang
One of the major obstacles hindering the treatment of lung cancer (LC) is chemoresistance; however, its mechanism remains unclear. The overexpression of the metastasis-associated in colon cancer 1 (MACC1) gene has been demonstrated to reverse chemoresistance. In the current study, the expression of MACC1 in LC cells with cisplatin resistance (Cis-Re) was investigated. Cisplatin-resistant cell sublines (A549/CR and H446/CR) were induced by stepwise escalation of cisplatin exposure. MTS and flow cytometry assays were performed to measure cell proliferation and apoptosis, respectively...
June 29, 2018: Acta Biochimica et Biophysica Sinica
Bingnan He, Xia Wang, Lai Wei, Baida Kong, Yuanxiang Jin, Xiaoxian Xie, Zhengwei Fu
The most widely used type II pyrethroid is β-cypermethrin (β-CYP), and 3-phenoxybenzoic acid (3-PBA) is one of its primary metabolites. Although CYP has been shown to pose toxic effects in some immune cells, as of now the immunotoxicity of CYP on immune progenitor cells has not been well studied. In this study, we evaluated the immunotoxicity of β-CYP and 3-PBA on the human promyelocytic leukemia cell line, HL-60. Both β-CYP and 3-PBA reduced cell viability. In addition, both β-CYP and 3-PBA stimulated the intrinsic apoptotic pathway in a dose- and time-dependent manner, while only β-CYP induced cell cycle arrest in G1 stage...
June 26, 2018: Acta Biochimica et Biophysica Sinica
Yingxiang Liu, Xuexue Ren, Jie Ke, Yan Zhang, Qing Wei, Zhaopeng Shi, Zhiying Ai, Zekun Guo
The ability to self-renew is one of the most important properties of embryonic stem (ES) cells. Pluripotin (SC1), a small molecule with high activity and low toxicity, promotes self-renewal in mouse ES cells. SC1 can noticeably change the morphology of retinoic acid (RA)-induced F9 embryonic carcinoma cells (F9 cells). However, in the long term, RA and SC1 together cause cell apoptosis. When being added after 18-24 h of RA-induced F9 cell differentiation, SC1 transitorily activated Nanog and Oct4. Both Nanog and Oct4 were downregulated when SC1 and RA were added simultaneously...
June 26, 2018: Acta Biochimica et Biophysica Sinica
Lixia Xu, Jialin Li, Zhongqiu Luo, Qiaoli Wu, Weijia Fan, Xiuhua Yao, Qingguo Li, Hua Yan, Jinhuan Wang
Multiple independent reports have demonstrated pericyte loss in both the hippocampus and cortex in human Alzheimer's disease (AD). The differentiation and recruitment of pericytes are the essential steps in vasculature development. However, the role of amyloid beta (Aβ) in pericyte differentiation has not yet been fully elucidated. In this study, we investigated the interaction between Aβ and differentiation of mesenchymal stem cells (MSCs) toward pericytes in culture. Our results showed that mice overexpressing Aβ-precursor protein (APP/PS1) exhibited the loss of pericytes compared with the control group mice, evidenced by the lack of desmin expression in the cortex of 12-month-old mice...
June 23, 2018: Acta Biochimica et Biophysica Sinica
Wenguang Chang, Junfang Teng
Demyelinating diseases, such as multiple sclerosis, are known to result from acute or chronic injury to the myelin sheath and inadequate remyelination. Its underlying molecular mechanisms, however, remain unclear. The transcription factor prospero homeobox 1 (Prox1) plays an essential role during embryonic development of the central nervous system and cell differentiation. Thus, we aimed to investigate the role of Prox1 in the survival and differentiation of oligodendrocytes. Cell viability was measured by MTT assay...
June 20, 2018: Acta Biochimica et Biophysica Sinica
Ruihua Ma, Pinwen Wu, Qiqing Shi, Dongli Song, Hao Fang
Mechanical ventilation (MV) is an important procedure for the treatment of patients with acute lung injury or acute respiratory distress syndrome in a clinical setting; however, MV can lead to severe complications, including ventilator-induced lung injury (VILI). Telocytes (TCs) can promote tissue repair following injury in the heart, kidneys, and other organs. The aim of this study was to investigate the role of TCs in VILI in mice and the associated mechanisms. By using in vivo studies in mice and in vitro studies in cells, we demonstrated that an airway injection of TCs can reduce the pulmonary inflammatory response and improve the lung function in mice with VILI and promote the proliferation of pulmonary vascular endothelial cells...
June 19, 2018: Acta Biochimica et Biophysica Sinica
Bo Feng, Taotao Zhang, Fenfang Wu, Shangwu Chen, Anlong Xu
No abstract text is available yet for this article.
June 19, 2018: Acta Biochimica et Biophysica Sinica
Mingzhu Tang, Liqun Lu, Zhen Huang, Linxi Chen
No abstract text is available yet for this article.
June 19, 2018: Acta Biochimica et Biophysica Sinica
Weina Wang, Aimei Wang, Guochang Luo, Fengqiao Ma, Xiaoming Wei, Yongyi Bi
Ischemia/reperfusion (I/R) is a major cause of acute kidney injury (AKI), along with delayed graft function, which can trigger chronic kidney injury by stimulating epithelial to mesenchymal transition (EMT) in the kidney canaliculus. Sphingosine 1-phosphate receptor 1 (S1P1) is a G protein-coupled receptor that is indispensable for vessel homeostasis. This study aimed to investigate the influence of S1P1 on the mechanisms underlying I/R-induced EMT in the kidney using in vivo and in vitro models. Wild-type (WT) and S1P1-overexpressing kidney canaliculus cells were subject to hypoxic conditions followed by reoxygenation in the presence or absence of FTY720-P, a potent S1P1 agonist...
June 13, 2018: Acta Biochimica et Biophysica Sinica
Fang Li, Junping Zhang, Jiqiang Guo, Yuan Jia, Yaping Han, Zhuanhua Wang
Breast cancer is one of the most common malignancies. It is necessary to identify new markers for predicting tumor progression and therapeutic molecular targets. It has been reported that CD147 is one of the most commonly expressed proteins in primary tumors and in metastatic cells. In this study, we investigated the role of CD147 in human breast cancer metastasis and invasion, and examined its underlying molecular mechanisms. Immunohistochemistry results revealed high expression of CD147 in human breast tumor tissues, which was positively correlated with the malignancy of breast cancer...
June 12, 2018: Acta Biochimica et Biophysica Sinica
Xiang Chen, Jiaqi Zhang, Ze Wang, Xu Xu, Bin Zhu
No abstract text is available yet for this article.
June 11, 2018: Acta Biochimica et Biophysica Sinica
Ningyu Huang, Xiaojuan Pei, Wenbo Lin, Jen-Fu Chiu, Tao Tao, Guanwu Li
NAD(P)H:quinone oxidoreductase 1 (NQO1), a phase II flavoenzyme that catalyzes reduction reactions to protect cells against electrophiles and oxidants, is involved in tumorigenesis. Altered methylation of the NQO1 gene has been observed and is speculated to result in aberrant NQO1 expression in rat cells undergoing chemical carcinogenesis, although this has not been proven experimentally. In this study, we first investigated the potential epigenetic mechanisms underlying the phenomenon of NQO1 differential expression in individual subclones of rat arsenic-transformed lung epithelial cells (TLECs)...
June 8, 2018: Acta Biochimica et Biophysica Sinica
Yifan Su, Hua Tian, Lijiang Wei, Guohui Fu, Ting Sun
Hypoxia-induced apoptosis plays an important role in cardiovascular diseases. Integrin β3 is one of the main integrin heterodimer receptors on the surface of cardiac myocytes. However, despite the important role that integrin β3 plays in the cardiovascular disease, its exact role in the hypoxia response remains unclear. Hence, in the present investigation we aimed to study the role of integrin β3 in hypoxia-induced apoptosis in H9C2 cells and primary rat myocardial cells. MTT assay, flow cytometry and TUNEL assay results showed that hypoxia inhibited cardiomyocyte proliferation and induced cardiomyocyte apoptosis...
May 23, 2018: Acta Biochimica et Biophysica Sinica
Yuanbo Zhao, Jay P Uhler
G-quadruplexes are higher-order nucleic acid structures formed in G-rich sequences in DNA or RNA. G-quadruplexes are distributed in many locations in the human genome, including promoter regions, and are viewed as promising therapeutic targets. Uncoupling protein-1 (UCP1) is a mitochondrial thermogenic gene critical for energy expenditure in the form of heat in the brown adipose tissue. UCP1 is only expressed during brown fat cell differentiation and is a candidate target for treating obesity. However, the regulation of UCP1 expression is not clear...
May 23, 2018: Acta Biochimica et Biophysica Sinica
Hongwen Chen, Yu Du, Hongwei Guo
No abstract text is available yet for this article.
May 22, 2018: Acta Biochimica et Biophysica Sinica
Qiuju Wu, Zekun Chen, Guihua Zhang, Wenhui Zhou, You Peng, Rong Liu, Ceshi Chen, Jing Feng
Breast cancer is one of the most common malignancies in women. ERα, PR, and HER2 triple negative breast cancer (TNBC) is the current research focus because of the lack of effective targeted therapies. In our study, lentivirus systems were used to overexpress EZH2 and miR-1301 in TNBC cell lines. Western blot analysis and RT-qPCR were used to detect the protein and microRNA levels. The TCGA and Kaplan Meier plotter databases were used to analyze the EZH2 and miR-1301 expression levels in breast cancer. The effect of miR-1301 overexpression on cell proliferation, migration and colony formation were determined by using the sulforhodamine B (SRB) assay, wound healing assay and colony formation assay, respectively...
May 22, 2018: Acta Biochimica et Biophysica Sinica
Xinchao Zhang, Yi Shi, Lingjun Song, Chang Shen, Qi Cai, Zhou Zhang, Jun Wu, Guohui Fu, Weiwei Shen
Idiopathic acquired pure red cell aplasia (PRCA) is a rare, autoimmune-related disease. This study aimed to describe the previously unidentified DNA alterations associated with PRCA. Here, next generation sequencing using a panel containing 295 critical genes was applied to detect potentially pathogenic mutations in four patients with PRCA. A total of 529 mutations were identified and further classified into three categories, namely, uncertain (n = 25), likely benign (n = 20) and benign (n = 484) mutations, based on the American College of Medical Genetics and Genomics (ACMG) 2015 guidelines and ClinVar database...
May 15, 2018: Acta Biochimica et Biophysica Sinica
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