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Acta Biochimica et Biophysica Sinica

Qiuju Wu, Zekun Chen, Guihua Zhang, Wenhui Zhou, You Peng, Rong Liu, Ceshi Chen, Jing Feng
Breast cancer is one of the most common malignancies in women. ERα, PR, and HER2 triple negative breast cancer (TNBC) is the current research focus because of the lack of effective targeted therapies. In our study, lentivirus systems were used to overexpress EZH2 and miR-1301 in TNBC cell lines. Western blot analysis and RT-qPCR were used to detect the protein and microRNA levels. The TCGA and Kaplan Meier plotter databases were used to analyze the EZH2 and miR-1301 expression levels in breast cancer. The effect of miR-1301 overexpression on cell proliferation, migration and colony formation were determined by using the sulforhodamine B (SRB) assay, wound healing assay and colony formation assay, respectively...
May 22, 2018: Acta Biochimica et Biophysica Sinica
Xinchao Zhang, Yi Shi, Lingjun Song, Chang Shen, Qi Cai, Zhou Zhang, Jun Wu, Guohui Fu, Weiwei Shen
Idiopathic acquired pure red cell aplasia (PRCA) is a rare, autoimmune-related disease. This study aimed to describe the previously unidentified DNA alterations associated with PRCA. Here, next generation sequencing using a panel containing 295 critical genes was applied to detect potentially pathogenic mutations in four patients with PRCA. A total of 529 mutations were identified and further classified into three categories, namely, uncertain (n = 25), likely benign (n = 20) and benign (n = 484) mutations, based on the American College of Medical Genetics and Genomics (ACMG) 2015 guidelines and ClinVar database...
May 15, 2018: Acta Biochimica et Biophysica Sinica
Xiangyuan Chen, Qichao Wu, Hui Jiang, Jiaqiang Wang, Yanjun Zhao, Yajun Xu, Minmin Zhu
Hyperglycemic memory occurs in diabetic cardiovascular complications, but the underlying mechanism remains to be elucidated. Although the depletion of SET8 leads to increased mitochondrial oxidative stress via increasing cellular reactive oxygen species (ROS) production, the role of SET8 in hyperglycemic memory-induced mitochondrial dysfunction is not well understood. Here, we investigated the role of SET8 in this setting. Our results showed that high glucose-induced vascular inflammation, ROS production and apoptosis remained at high levels even when glucose returned to normal level...
May 14, 2018: Acta Biochimica et Biophysica Sinica
Jing Zhang, Yan Zhang, Xiaoping Tan, Qing Zhang, Chaoyong Liu, Yali Zhang
MicroRNAs (miRNAs), some small non-coding RNAs that regulate gene expression at the posttranscriptional level, are always aberrantly expressed in carcinomas. In this study, we found that miR-23b-3p was remarkably up-regulated in human esophageal squamous cell carcinoma cells and tissues. Moreover, miR-23b-3p could induce the proliferation, invasion, and metastasis in vitro. EBF3 was identified as the direct downstream target gene of miR-23b-3p and ectogenic EBF3 could strongly inhibit the proliferation, invasion, and metastasis in vitro...
May 10, 2018: Acta Biochimica et Biophysica Sinica
Kai Zhang, Zhen Shen, Xianjun Liang, Tongjun Liu, Tiejun Wang, Yang Jiang
No abstract text is available yet for this article.
May 2, 2018: Acta Biochimica et Biophysica Sinica
Wu Zhong, Yu Zhao, Ye Tian, Muhu Chen, Xue Lai
Closed vascular injuries have danger of developing critical tissue ischemia with a high risk of amputation and limb loss. However, limited effective strategies exist at present. In this study, we investigate the role of hepatocyte growth factor (HGF) on apoptosis of vascular endothelial cells (VECs). First, apoptosis of VECs was induced by hypoxia treatment with or without HGF. Annexin V-7AAD apoptosis assay revealed that HGF overexpression significantly reduced VEC apoptosis. Then a closed peripheral vascular injury animal model was created by balloon catheter in female New Zealand rabbit...
April 30, 2018: Acta Biochimica et Biophysica Sinica
Yingying Zhang, Linhai Cong, Jiayan He, Yu Wang, Yingying Zou, Zhihong Yang, Yuexin Hu, Shiwen Zhang, Xiaoguang He
Hypopharyngeal carcinoma (HC) is one of the most malignant tumors in the upper aerodigestive tract. Currently, there are no effective treatments for HC. Gold nanoparticles (AuNPs) are a promising tool that can be used for plasmonic photothermal therapy (PPTT), which refers to the use of electromagnetic radiation, most often in near infrared (NIR) region, for the treatment of various medical conditions including cancer. AuNPs have been proved to be a promising tool for NIR spectroscopy-mediated photothermal therapies...
April 27, 2018: Acta Biochimica et Biophysica Sinica
Qian Jia, Yun Lin, Xuejing Gou, Lei He, Dong Shen, Dongni Chen, Wei Xie, Yongjun Lu
The gram-negative bacterium Legionella pneumophila invades human's lung and causes Legionnaires' disease. To benefit its survival and replication in cellular milieu, L. pneumophila secrets at least 330 effector proteins into host cells. We found that the effector WipA has the protein tyrosine phosphatase (PTP) activity but does not depend on the classical CX5R motif for activity, suggesting that WipA is an unconventional PTP. Meanwhile, the presence of three other highly conserved motifs typically seen in protein serine/threonine phosphatases and the poor inhibition of WipA activity by okadaic acid led us to propose that WipA is a bacterial protein phosphatase...
April 26, 2018: Acta Biochimica et Biophysica Sinica
Heng Zhang, Yunping Jiao, Chunyang Shi, Xiao Song, Ying Chang, Yong Ren, Xiaolin Shi
Ovarian cancer is a common and lethal cancer affecting women globally. Berbamine is a natural compound from the plant Berberis amurensis, which is used in Chinese traditional medicine. Recent studies have shown the anti-tumor effects of berbamine in several types of cancers but not in ovarian cancer. In the present study, we investigated the potential anti-tumor effects of berbamine in ovarian cancer and explored the underlying molecular mechanisms. Berbamine suppressed the cell viability of ovarian cancer cells in a concentration-dependent manner as revealed by methyl thiazolyl tetrazolium assay...
April 26, 2018: Acta Biochimica et Biophysica Sinica
Jieqiong Ding, Zhiwei Zhao, Jia Song, Binghua Luo, Lingping Huang
Although doxorubicin has become a key drug in cancer treatment, the resistance of colorectal carcinoma to doxorubicin is a major problem in clinical practice. F-box and WD repeat domain-containing 7 (FBXW7) plays important roles in human cancers and is one of the major causes of drug resistance. The miR-223/FBXW7 pathway has been reported to be a crucial clue to the mechanism of chemoresistance in many human cancers, such as gastric cancer, breast cancer, and non-small cell lung cancer. However, it is unclear whether similar mechanisms of doxorubicin resistance are involved in colorectal cancer (CRC)...
April 26, 2018: Acta Biochimica et Biophysica Sinica
Peng Wang, Suli Zhang, Jie Ren, Li Yan, Lina Bai, Li Wang, Pengli Wang, Jingwei Bian, Xiaochen Yin, Huirong Liu
Autoantibodies against angiotensin II Type 1 receptor (AT1-AA) are routinely detected in the serum of preeclampsia patients, which results in an increase in vascular tone and an elevation in intracellular calcium concentration of rat vascular smooth muscle (VSM). The big conductance calcium-activated potassium channels (BKCa channels) account for the dominant outward currents in VSMCs, contributing to membrane hyperpolarization and vasodilation. In the present study, we investigated the effect of AT1-AA on BKCa channels...
April 25, 2018: Acta Biochimica et Biophysica Sinica
Libin Song, Xiangyuan Chen, Peng Wang, Song Gao, Chao Qu, Luming Liu
Recent studies have suggested that sonic Hedgehog (Shh) signaling pathway is aberrantly activated in cancer stem cells (CSCs). A seven-herb Chinese medicinal formula composed of Amorphophallus rivieri Durieu, Oldenlandia diffusa (Wild) Roxb, Scutellaria barbata D. Don, Gynostemma pentaphyllum (Thunb.) Mak and Amomum cardamomum L, i.e. Qingyihuaji (QYHJ) formula, has been shown to inhibit proliferation of pancreatic CSCs by inhibiting Shh signaling pathway and thereby prolong the overall survival of pancreatic cancer patients...
April 25, 2018: Acta Biochimica et Biophysica Sinica
Wenqin Xiao, Bo Li, Xi Sun, Dandan Yu, Yongsheng Xie, Huiqun Wu, Shuaikang Chang, Yunfei Zhou, Houcai Wang, Xiucai Lan, Zhijian Xu, Jumei Shi, Weiliang Zhu
DCZ3301, a novel aryl-guanidino compound, was previously found to have potent anti-tumor activity in myeloma and B-cell lymphoma. In the present study, we investigated the effects of DCZ3301 on T-cell leukemia/lymphoma cells both in vitro and in vivo via cell proliferation, cell cycle analysis, apoptosis assay, mitochondrial membrane potential (MMP) assay, western blot analysis and tumor xenograft models. We found that DCZ3301 inhibited the viability of T-cell leukemia/lymphoma cells in a dose- and time-dependent manner...
April 23, 2018: Acta Biochimica et Biophysica Sinica
Haiyan Zhang, Xiaoming Zhang, Xiaodong Chen, Wei Zhang, Jiang Xian, Xia Zhou, Jun Yang, Jie Wang
No abstract text is available yet for this article.
April 23, 2018: Acta Biochimica et Biophysica Sinica
Yinghui Xie, Tanglong Yuan, Yanqing Qin, Zhonghui Weng, Jing Fang
Prolyl hydroxylases (PHD1-3) hydroxylate hypoxia inducible factor α (HIFα), leading to HIFα ubiquitination and degradation. Recent studies indicated that administration of generic inhibitors of PHDs improved mice colitis, suggesting that suppression of PHD activity by these inhibitors may be a potential strategy for the treatment of inflammatory bowel diseases. However, the exact role of each member of PHD family in homeostasis of intestinal epithelium remains elusive. The aim of this work is to study the possible role of PHD2 by using mice with genetic ablation of Phd2 in intestinal epithelial cells (IECs)...
April 23, 2018: Acta Biochimica et Biophysica Sinica
Ji Hoon Oh, Clara Yuri Kim, Ji-Yeon Lee, Myoung Hee Kim
During the development of an embryo, the initiation of the collinear expression of Hox genes is essential for the proper formation of the anteroposterior body axis. Retinoic acid (RA), a natural derivative of vitamin A, plays a role in vertebrate development by regulating Hox gene expression. CCCTC-binding factor (CTCF), an insulator protein that controls gene transcription, also regulates the expression of Hox genes by binding to the CTCF-binding sites (CBSs). It has been reported that upon RA signaling, retinoic acid response elements (RAREs) located in the Hox clusters become occupied...
April 23, 2018: Acta Biochimica et Biophysica Sinica
Kai Zhang, Qionglin Zhou, Yu Guo, Linxi Chen, Lanfang Li
No abstract text is available yet for this article.
April 19, 2018: Acta Biochimica et Biophysica Sinica
Jiajie Li, Guangyao Tang, Wenjuan Qin, Rentan Yang, Rongji Ma, Bin Ma, Jianfeng Wei, Hailong Lv, Yufeng Jiang
Cystic echinococcosis is a severe parasitic disease that commonly affects the liver and causes abscesses or rupture into the surrounding tissues, leading to multiple complications, such as shock, severe abdominal pain, and post-treatment abscess recurrence. Currently, there are no efficient measures to prevent these complications. We previously confirmed that arsenic trioxide (As2O3) exhibited in vitro cytotoxicity against Echinococcus granulosus protoscoleces. In the present study, we aimed to explore the mechanism of As2O3-induced E...
April 19, 2018: Acta Biochimica et Biophysica Sinica
Qianfeng Zhuang, Weiping Luo, Mingran Zhang, Min Fan, Hao Lu, Renfang Xu, Xiaozhou He
Calpain small subunit 1 (Capn4) has been shown to correlate with the metastasis/invasion of clear cell renal cell carcinoma (ccRCC). This study aimed to further elucidate the molecular mechanisms underlying Capn4-mediated ccRCC progression. The messenger RNA expression levels in ccRCC cells were measured by quantitative real-time PCR. The effects of Capn4 on cell adhesion, invasion, and migration were examined by cell adhesion assay, cell invasion assay, and wound-healing assay, respectively. The protein levels were detected by western blot analysis...
April 10, 2018: Acta Biochimica et Biophysica Sinica
Xu Wang, Sheng Wu, Wenbing Jin, Bin Xu, Gongli Tang, Hua Yuan
No abstract text is available yet for this article.
April 6, 2018: Acta Biochimica et Biophysica Sinica
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