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Plant Biotechnology Journal

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https://www.readbyqxmd.com/read/28317293/a-new-location-to-split-cre-recombinase-for-protein-fragment-complementation
#1
Maryam Rajaee, David W Ow
We have previously described a recombinase-mediated gene stacking system in which the Cre recombinase is used to remove lox-site flanked DNA no longer needed after each round of Bxb1 integrase-mediated site-specific integration. The Cre recombinase can be conveniently introduced by hybridization with a cre-expressing plant. However, maintaining an efficient cre-expressing line over many generations can be a problem, as high production of this DNA binding protein might interfere with normal chromosome activities...
March 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28317264/a-collection-of-enhancer-trap-insertional-mutants-for-functional-genomics-in-tomato
#2
Fernando Pérez-Martín, Fernando J Yuste-Lisbona, Benito Pineda, María Pilar Angarita-Díaz, Begoña García-Sogo, Teresa Antón, Sibilla Sánchez, Estela Giménez, Alejandro Atarés, Antonia Fernández-Lozano, Ana Ortíz-Atienza, Manuel García-Alcázar, Laura Castañeda, Rocío Fonseca, Carmen Capel, Geraldine Goergen, Jorge Sánchez, Jorge L Quispe, Juan Capel, Trinidad Angosto, Vicente Moreno, Rafael Lozano
With the completion of genome sequencing projects, the next challenge is to close the gap between gene annotation and gene functional assignment. Genomic tools to identify gene functions are based on the analysis of phenotypic variations between a wild-type and its mutant; hence, mutant collections are a valuable resource. In this sense, T-DNA collections allow for an easy and straightforward identification of the tagged gene, serving as the basis of both forward and reverse genetic strategies. This study reports on the phenotypic and molecular characterization of an enhancer trap T-DNA collection in tomato (Solanum lycopersicum L...
March 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28316134/feruloylation-and-structure-of-arabinoxylan-in-wheat-endosperm-cell-walls-from-rnai-lines-with-suppression-of-genes-responsible-for-backbone-synthesis-and-decoration
#3
Jackie Freeman, Jane L Ward, Ondrej Kosik, Alison Lovegrove, Mark D Wilkinson, Peter R Shewry, Rowan A C Mitchell
Arabinoxylan (AX) is the major component of the cell walls of wheat grain (70% in starchy endosperm) and is an important determinant of end-use qualities affecting food processing, use for animal feed and distilling and is a major source of dietary fibre in the human diet. AX is a heterogeneous polysaccharide composed of fractions which can be sequentially extracted by water (WE-AX), then xylanase action (XE-AX) leaving an unextractable (XU-AX) fraction. We determined arabinosylation and feruloylation of AX in these fractions in both wild-type wheat and RNAi lines with decreased AX content (TaGT43_2 RNAi, TaGT47_2 RNAi) or decreased arabinose 3-linked to mono-substituted xylose (TaXAT1 RNAi)...
March 17, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28301719/a-reconfigured-kennedy-pathway-which-promotes-efficient-accumulation-of-medium-chain-fatty-acids-in-leaf-oils
#4
Kyle B Reynolds, Matthew C Taylor, Darren P Cullerne, Christopher L Blanchard, Craig C Wood, Surinder P Singh, James R Petrie
Medium chain fatty acids (MCFA, C6-14 fatty acids) are an ideal feedstock for biodiesel and broader oleochemicals. In recent decades, several studies have used transgenic engineering to produce MCFA in seeds oils, though these modifications result in unbalance membrane lipid profiles that impair oil yields and agronomic performance. Given the ability to engineer non-seed organs to produce oils, we have previously demonstrated that MCFA profiles can be produced in leaves, but this also results in unbalanced membrane lipid profiles and undesirable chlorosis and cell death...
March 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28301718/the-wheat-lr34-multi-pathogen-resistance-gene-confers-resistance-to-anthracnose-and-rust-in-sorghum
#5
Wendelin Schnippenkoetter, Clive Lo, Guoquan Liu, Katherine Dibley, Wai Lung Chan, Jodie White, Ricky Milne, Alexander Zwart, Eunjung Kwong, Beat Keller, Ian Godwin, Simon G Krattinger, Evans Lagudah
The ability of the wheat Lr34 multi-pathogen resistance gene (Lr34res) to function across a wide taxonomic boundary was investigated in transgenic Sorghum bicolor. Increased resistance to sorghum rust and anthracnose disease symptoms following infection with the biotrophic pathogen Puccinia purpurea and the hemibiotroph Colletotrichum sublineolum respectively occurred in transgenic plants expressing the Lr34res ABC transporter. Transgenic sorghum lines that highly expressed the wheat Lr34res gene exhibited immunity to sorghum rust compared to the low expressing single copy Lr34res genotype that conferred partial resistance...
March 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28301713/population-structure-and-genetic-basis-of-the-agronomic-traits-of-upland-cotton-in-china-revealed-by-a-genome-wide-association-study-using-high-density-snps
#6
Cong Huang, Xinhui Nie, Chao Shen, Chunyuan You, Wu Li, Wenxia Zhao, Xianlong Zhang, Zhongxu Lin
Gossypium hirsutum L. represents the largest source of textile fibre, and China is one of the largest cotton producing and consuming countries in the world. To investigate the genetic architecture of the agronomic traits of upland cotton in China, a diverse and nation-wide population containing 503 G. hirsutum accessions was collected for a genome-wide association study (GWAS) on 16 agronomic traits. The accessions were planted in four places from 2012 to 2013 for phenotyping. The CottonSNP63K array and a published high-density map based on this array were used for genotyping...
March 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28301712/hypoxia-responsive-erfs-involved-in-post-deastringency-softening-of-persimmon-fruit
#7
Miao-Miao Wang, Qing-Gang Zhu, Chu-Li Deng, Zheng-Rong Luo, Ning-Jing Sun, Donald Grierson, Xue-Ren Yin, Kun-Song Chen
Removal of astringency by endogenously formed acetaldehyde, achieved by postharvest anaerobic treatment, is of critical importance for many types of persimmon fruit. Although an anaerobic environment accelerates de-astringency it also has the deleterious effect of promoting excessive softening, reducing shelf-life and marketability. Some hypoxia-responsive ethylene response factor (ERFs) participate in anaerobic de-astringency, but their role in accelerated softening was unclear. Undesirable rapid softening induced by high CO2 (95%) was ameliorated by adding the ethylene inhibitor 1-MCP (1 μl L(-1) ), resulting in reduced astringency while maintaining firmness, suggesting that CO2 -induced softening involves ethylene signalling...
March 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28278368/dynamic-metabolic-responses-of-brown-planthoppers-towards-susceptible-and-resistant-rice-plants
#8
Caixiang Liu, Ba Du, Fuhua Hao, Hehua Lei, Qianfen Wan, Guangcun He, Yulan Wang, Huiru Tang
Brown planthopper (Nilaparvata lugens Stål, BPH) causes huge economic losses in rice-growing regions and new strategies for combating BPH are required. To understand how BPHs respond towards BPH-resistant plants, we systematically analyzed the metabolic differences between BPHs feeding on the resistant and susceptible plants using NMR and GC-FID/MS. We also measured the expression of some related genes involving glycolysis and biosyntheses of trehalose, amino acids, chitin and fatty acids using real-time PCR...
March 9, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28278353/bt-rice-in-china-focusing-the-non-target-risk-assessment
#9
Yunhe Li, Qingling Zhang, Qingsong Liu, Michael Meissle, Yan Yang, Yanan Wang, Hongxia Hua, Xiuping Chen, Yufa Peng, Jörg Romeis
Bt rice can control yield losses caused by lepidopteran pests but may also harm non-target species and reduce important ecosystem services. A comprehensive data set on herbivores, natural enemies, and their interactions in Chinese rice fields was compiled. This together with an analysis of the Cry protein content in arthropods collected from Bt rice in China indicated which non-target species are most exposed to the insecticidal protein and should be the focus of regulatory risk assessment. This article is protected by copyright...
March 9, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28273400/fitness-of-bt-resistant-cabbage-loopers-on-bt-cotton-plants
#10
Guillaume Tetreau, Ran Wang, Ping Wang
Development of resistance to the insecticidal toxins from Bacillus thuringiensis (Bt) in insects is the major threat to the continued success of transgenic Bt-crops in agriculture. The fitness of Bt-resistant insects on Bt- and non-Bt-plants is a key parameter that determines the development of Bt-resistance in insect populations. In this study, a comprehensive analysis of the fitness of Bt-resistant Trichoplusia ni strains on Bt-cotton leaves was conducted. The Bt-resistant T. ni strains carried two genetically independent mechanisms of resistance to Bt toxins Cry1Ac and Cry2Ab...
March 8, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28273388/enhanced-transport-of-plant-produced-rabies-single-chain-antibody-rvg-peptide-fusion-protein-across-an-in-cellulo-blood-brain-barrier-device
#11
Waranyoo Phoolcharoen, Christophe Prehaud, Craig J van Dolleweerd, Leonard Both, Anaelle da Costa, Monique Lafon, Julian K-C Ma
The biomedical applications of antibody engineering are developing rapidly and have been expanded to plant expression platforms. In the present study, we have generated a novel antibody molecule in planta for targeted delivery across the blood-brain barrier (BBB). Rabies virus (RABV) is a neurotropic virus for which there is no effective treatment after entry into the central nervous system (CNS). This study investigated the use of a RABV glycoprotein peptide sequence to assist delivery of a rabies neutralising single-chain antibody (ScFv) across an in cellulo model of human BBB...
March 8, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28258966/overexpression-of-populus-trichocarpa-cyp85a3-promotes-growth-and-biomass-production-in-transgenic-trees
#12
Yan-Li Jin, Ren-Jie Tang, Hai-Hai Wang, Chun-Mei Jiang, Yan Bao, Yang Yang, Mei-Xia Liang, Fanjing Kong, Bei Li, Hong-Xia Zhang
Brassinosteroids (BRs) are essential hormones that play crucial roles in plant growth, reproduction and response to abiotic and biotic stress. In Arabidopsis, AtCYP85A2 works as a bifunctional cytochrome P450 monooxygenase to catalyze the conversion of castasterone (CS) to brassinolide (BL), a final rate-limiting step in the BR biosynthetic pathway. Here, we report the functional characterizations of PtCYP85A3, one of the three AtCYP85A2 homologous genes from Populus trichocarpa. PtCYP85A3 shares the highest similarity with AtCYP85A2 and can rescue the retarded-growth phenotype of the Arabidopsis cyp85a2-2 and tomato d(x) mutants...
March 4, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28244201/overexpression-of-oserf48-causes-regulation-of-oscml16-a-calmodulin-like-protein-gene-that-enhances-root-growth-and-drought-tolerance
#13
Harin Jung, Pil Joong Chung, Su-Hyun Park, Mark Christian Felipe Reveche Redillas, Youn Shic Kim, Joo-Won Suh, Ju-Kon Kim
The AP2/ERF family is a plant-specific transcription factor (TF) family whose members have been associated with various developmental processes and stress tolerance. Here, we functionally characterized the drought-inducible OsERF48, a group Ib member of the rice ERF family with four conserved motifs, CMI-1, -2, -3 and -4. A transactivation assay in yeast revealed that the C-terminal CMI-1 motif was essential for OsERF48 transcriptional activity. When OsERF48 was overexpressed in an either a root-specific (ROX(OsERF48) ) or whole-body (OX(OsERF48) ) manner, transgenic plants showed a longer and denser root phenotype compared to the nontransgenic (NT) controls...
February 28, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28233945/activation-of-secondary-cell-wall-biosynthesis-by-mir319-targeted-tcp4-transcription-factor
#14
Xudong Sun, Wang Chongde, Xiang Nan, Li Xiong, Yang Shihai, Du Jiancan, Yongping Yang, Yunqiang Yang
The overexpression of miR319 in plants results in delayed senescence, and high levels of miR319-targeted TCP4 transcription factor causes premature onset of this process. However, the underlying mechanisms of this pathway remain elusive. Here, we found that miR319 overexpression results in a decrease in TCP4 abundance and secondary cell wall formation in the stem. Conversely, constitutive expression of miR319-resistant TCP4 (rTCP4) promotes secondary cell wall formation, indicating that miR319-mediated TCP4 controls secondary cell wall formation during development...
February 24, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28226420/posnar2-1-osnar2-1-expression-enhances-nitrogen-uptake-efficiency-and-grain-yield-in-transgenic-rice-plants
#15
Jingguang Chen, Xiaoru Fan, Kaiyun Qian, Yong Zhang, Miaoquan Song, Yu Liu, Guohua Xu, Xiaorong Fan
The nitrate (NO3(-) ) transporter has been selected as an important gene maker in the process of environmental adoption in rice cultivars. In this work, we transfered another native OsNAR2.1 promoter with driving OsNAR2.1 gene into rice plants. The transgenic lines with exogenous pOsNAR2.1:OsNAR2.1 constructs showed enhanced OsNAR2.1 expression level, compared with wild type (WT), and (15) N influx in roots increased 21-32% in response to 0.2 mM and 2.5 mM (15) NO3(-) and 1.25 mM (15) NH4(15) NO3 . Under these three N conditions, the biomass of the pOsNAR2...
February 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28218981/the-genetic-architecture-of-amino-acids-dissection-by-association-and-linkage-analysis-in-maize
#16
Min Deng, Dongqin Li, Jingyun Luo, Yingjie Xiao, Haijun Liu, Qingchun Pan, Xuehai Zhang, Minliang Jin, Mingchao Zhao, Jianbing Yan
Amino acids are both constituents of proteins, providing the essential nutrition for humans and animals, and signaling molecules regulating the growth and development of plants. Most cultivars of maize are deficient in essential amino acids such as lysine and tryptophan. Here, we measured the levels of 17 different total amino acids, and created 48 derived traits in mature kernels from a maize diversity inbred collection and three recombinant inbred line (RIL) populations. By GWAS, 247 and 281 significant loci were identified in two different environments, 5...
February 20, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28218978/biochemical-characterization-of-metabolism-based-atrazine-resistance-in-amaranthus-tuberculatus-and-identification-of-an-expressed-gst-associated-with-resistance
#17
Anton F Evans, Sarah R O'Brien, Rong Ma, Aaron G Hager, Chance W Riggins, Kris N Lambert, Dean E Riechers
Rapid detoxification of atrazine in naturally tolerant crops such as maize (Zea mays) and grain sorghum (Sorghum bicolor) results from glutathione S-transferase (GST) activity. In previous research, two atrazine-resistant waterhemp (Amaranthus tuberculatus) populations from Illinois, U.S.A. (designated ACR and MCR) displayed rapid formation of atrazine-glutathione (GSH) conjugates, implicating elevated rates of metabolism as the resistance mechanism. Our main objective was to utilize protein purification combined with qualitative proteomics to investigate the hypothesis that enhanced atrazine detoxification, catalyzed by distinct GSTs, confers resistance in ACR and MCR...
February 20, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28218975/stacking-transgenic-event-das-%C3%A3-15%C3%A3-7-1-alters-maize-composition-less-than-traditional-breeding
#18
Rod A Herman, Brandon J Fast, Peter N Scherer, Alyssa M Brune, Denise T de Cerqueira, Barry W Schafer, Ricardo D Ekmay, George G Harrigan, Greg A Bradfisch
The impact of crossing ("stacking") genetically modified (GM) events on maize-grain biochemical composition was compared with the impact of generating non-GM hybrids. The compositional similarity of seven GM stacks containing event DAS-Ø15Ø7-1 and their matched non-GM near-isogenic hybrids (iso-hybrids) was compared with the compositional similarity of concurrently grown non-GM hybrids and these same iso-hybrids. Scatter plots were used to visualize comparisons among hybrids and a coefficient of identity (percent of variation explained by line of identity) was calculated to quantify the relationships within analyte profiles...
February 20, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28207991/high-yield-secretion-of-recombinant-proteins-from-the-microalga-chlamydomonas-reinhardtii
#19
E M Ramos-Martinez, L Fimognari, Y Sakuragi
Microalga-based biomanufacturing of recombinant proteins is attracting growing attention due to its advantages in safety, metabolic diversity, scalability, and sustainability. Secretion of recombinant proteins can accelerate the use of microalgal platforms by allowing post-translational modifications and easy recovery of products from the culture media. However, currently, the yields of secreted recombinant proteins are low, which hampers the commercial application of this strategy. This study aimed at expanding the genetic tools for enhancing secretion of recombinant proteins in Chlamydomonas reinhardtii, a widely used green microalga as a model organism and a potential industrial biotechnology platform...
February 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28199783/next-generation-transgenic-cotton-pyramiding-rnai-and-bt-counters-insect-resistance
#20
Mi Ni, Wei Ma, Xiaofang Wang, Meijing Gao, Yan Dai, Xiaoli Wei, Lei Zhang, Yonggang Peng, Shuyuan Chen, Lingyun Ding, Yue Tian, Jie Li, Haiping Wang, Xiaolin Wang, Guowang Xu, Wangzhen Guo, Yihua Yang, Yidong Wu, Shannon Heuberger, Bruce E Tabashnik, Tianzhen Zhang, Zhen Zhu
Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant "pyramids" producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross-resistance and antagonism between Bt toxins limits the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi)...
February 15, 2017: Plant Biotechnology Journal
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