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Plant Biotechnology Journal

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https://www.readbyqxmd.com/read/30019807/simultaneous-crispr-cas9-mediated-editing-of-cassava-eif4e-isoforms-ncbp-1-and-ncbp-2-reduces-cassava-brown-streak-disease-symptom-severity-and-incidence
#1
Michael A Gomez, Z Daniel Lin, Theodore Moll, Raj Deepika Chauhan, Luke Hayden, Kelley Renninger, Getu Beyene, Nigel J Taylor, J Carrington, B Staskawicz, R Bart
Cassava brown streak disease (CBSD) is a major constraint on cassava yields in East and Central Africa and threatens production in West Africa. CBSD is caused by two species of positive sense RNA viruses belonging to the family Potyviridae, genus Ipomovirus: Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Diseases caused by the family Potyviridae require the interaction of viral genome-linked protein (VPg) and host eukaryotic translation initiation factor 4E (eIF4E) isoforms...
July 18, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29999579/multi-omics-analyses-reveal-epigenomics-basis-for-cotton-somatic-embryogenesis-through-successive-regeneration-acclimation-sra-process
#2
Jianying Li, Maojun Wang, Yajun Li, Qinghua Zhang, Keith Lindsey, Henry Daniell, Shuangxia Jin, Xianlong Zhang
Plant regeneration via somatic embryogenesis is time-consuming and highly genotype-dependent. The plant somatic embryogenesis process provokes many epigenetics changes including DNA methylation and histone modification. Recently, an elite cotton Jin668, with an extremely high regeneration ability, was developed from its maternal inbred Y668 cultivar using a Successive Regeneration Acclimation (SRA) strategy. To reveal the underlying mechanism of SRA, we carried out a genome-wide single-base resolution methylation analysis for non-embryogenic calluses (NECs), ECs, somatic embryos (SEs) during the somatic embryogenesis procedure and the leaves of regenerated offspring plants...
July 12, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29993179/plant-made-e2-glycoprotein-single-dose-vaccine-protects-pigs-against-classical-swine-fever
#3
Richard C Laughlin, Rachel Madera, Yair Peres, Brian R Berquist, Lihua Wang, Sterling Buist, Yulia Burakova, Sreenath Palle, Chungwon J Chung, Max V Rasmussen, Erica Martel, David A Brake, John G Neilan, Sara D Lawhon, L Garry Adams, Jishu Shi, Sylvain Marcel
Classical Swine Fever Virus (CSFV) causes classical swine fever, a highly contagious hemorrhagic fever affecting both feral and domesticated pigs. Outbreaks of CSF in Europe, Asia, Africa, and South America had significant adverse impacts on animal health, food security and the pig industry. The disease is generally contained by prevention of exposure through import restrictions (e.g. banning import of live pigs and pork products), localized vaccination programs, and culling of infected or at-risk animals, often at very high cost...
July 11, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29992702/the-red-bayberry-genome-and-genetic-basis-of-sex-determination
#4
Hui-Min Jia, Hui-Juan Jia, Qing-Le Cai, Yan Wang, Hai-Bo Zhao, Wei-Fei Yang, Guo-Yun Wang, Ying-Hui Li, Dong-Liang Zhan, Yu-Tong Shen, Qing-Feng Niu, Le Chang, Jie Qiu, Lan Zhao, Han-Bing Xie, Wan-Yi Fu, Jing Jin, Xiong-Wei Li, Yun Jiao, Chao-Chao Zhou, Ting Tu, Chun-Yan Chai, Jing-Long Gao, Long-Jiang Fan, Eric van de Weg, Jun-Yi Wang, Zhong-Shan Gao
Morella rubra, red bayberry, is an economically important fruit tree in south China. Here, we assembled the first high-quality genome for both a female and a male individual of red bayberry. The genome size was 313-Mb, and 90% sequences were assembled into eight pseudo chromosome molecules, with 32,493 predicted genes. By whole genome comparison between the female and male and association analysis with sequences of bulked and individual DNA samples from female and male, a 59-Kb region determining female was identified and located on distal end of pseudo-chromosome 8, which contains abundant transposable element and seven putative genes, four of them are related to sex floral development...
July 10, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29979490/engineering-linear-branched-chain-triterpene-metabolism-in-monocots
#5
Chase Kempinski, Zuodong Jiang, Garrett Zinck, Shirley J Sato, Zhengxiang Ge, Thomas E Clemente, Joe Chappell
Triterpenes are thirty carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to yield farnesyl diphosphate (FPP), catalyzed by FPP synthase (FPS). Squalene synthase (SQS) condenses two molecules of FPP to generate the symmetrical product squalene, the first committed precursor to sterols and most other triterpenes...
July 6, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29979486/engineering-triterpene-metabolism-in-the-oilseed-of-arabidopsis-thaliana
#6
Chase Kempinski, Joe Chappell
Squalene and botryococcene are linear, hydrocarbon triterpenes that have industrial and medicinal values. While natural sources for these compounds exist, there is a pressing need for robust, renewable production platforms. Oilseeds are an excellent target for heterologous production because of their roles as natural storage repositories and their capacity to produce precursors from photosynthetically-derived carbon. We generated transgenic Arabidopsis thaliana plants using a variety of engineering strategies (subcellular targeting and gene-stacking) to assess the potential for oilseeds to produce these two compounds...
July 6, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29972722/activities-and-specificities-of-crispr-cas9-and-cas12a-nucleases-for-targeted-mutagenesis-in-maize
#7
Keunsub Lee, Yingxiao Zhang, Benjamin P Kleinstiver, Jimmy A Guo, Martin J Aryee, Jonah Miller, Aimee Malzahn, Scott Zarecor, Carolyn J Lawrence-Dill, J Keith Joung, Yiping Qi, Kan Wang
CRISPR-Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing tools in plants. In this work, we compared their activities by targeting maize glossy2 gene coding region that has overlapping sequences recognized by both nucleases. We introduced constructs carrying SpCas9-guide RNA (gRNA) and LbCas12a-CRISPR RNA (crRNA) into maize inbred B104 embryos using Agrobacterium-mediated transformation. On-target mutation analysis showed that 90-100% of the Cas9-edited T0 plants carried indel mutations and 63-77% of them were homozygous or biallelic mutants...
July 4, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29969180/crispr-cas9-mediated-knockout-of-six-glycosyltransferase-genes-in-nicotiana-benthamiana-for-the-production-of-recombinant-proteins-lacking-%C3%AE-1-2-xylose-and-core-%C3%AE-1-3-fucose
#8
Julia Jansing, Markus Sack, Sruthy Maria Augustine, Rainer Fischer, Luisa Bortesi
Plants offer fast, flexible and easily scalable alternative platforms for the production of pharmaceutical proteins, but differences between plant and mammalian N-linked glycans, including the presence of β-1,2-xylose and core α-1,3-fucose residues in plants, can affect the activity, potency and immunogenicity of plant-derived proteins. Nicotiana benthamiana is widely used for the transient expression of recombinant proteins so it is desirable to modify the endogenous N-glycosylation machinery to allow the synthesis of complex N-glycans lacking β-1,2-xylose and core α-1,3-fucose...
July 3, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29949229/the-auxin-receptor-tir1-homolog-pagfbl1-regulates-adventitious-rooting-through-interactions-with-aux-iaa28-in-populus
#9
Wenbo Shu, Houjun Zhou, Cheng Jiang, Shutang Zhao, Liuqiang Wang, Quanzi Li, Zhangqi Yang, Andrew Groover, Meng-Zhu Lu
Adventitious roots occur naturally in many species, and can also be induced from explants of some tree species including Populus, providing an important means of clonal propagation. Auxin has been identified as playing a crucial role in adventitious root formation, but the associated molecular regulatory mechanisms need to be elucidated. In this study, we examined the role of PagFBL1, the hybrid poplar (Populus alba × P. glandulosa clone 84K) homolog of Arabidopsis auxin receptor TIR1, in adventitious root formation in poplar...
June 27, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29947466/genetic-architecture-underlying-the-lignin-biosynthesis-pathway-involves-non-coding-rnas-and-transcription-factors-for-growth-and-wood-properties-in-populus
#10
Mingyang Quan, Qingzhang Du, Liang Xiao, Wenjie Lu, Longxin Wang, Jianbo Xie, Yuepeng Song, Baohua Xu, Deqiang Zhang
Lignin provides structural support in perennial woody plants and is a complex phenolic polymer derived from phenylpropanoid pathway. Lignin biosynthesis is regulated by coordinated networks involving transcription factors (TFs), microRNAs (miRNAs), and long noncoding RNAs (lncRNAs). However, the genetic networks underlying the lignin biosynthesis pathway for tree growth and wood properties remains unknown. Here, we used association genetics (additive, dominant, and epistasis) and expression quantitative trait nucleotide (eQTN) mapping to decipher the genetic networks for tree growth and wood properties in 435 unrelated individuals of Populus tomentosa...
June 27, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29947463/influence-of-isopropylmalate-synthase-osipms1-on-seed-vigor-associated-with-amino-acid-and-energy-metabolism-in-rice
#11
Yongqi He, Jinping Cheng, Ying He, Bin Yang, Yanhao Cheng, Can Yang, Hongsheng Zhang, Zhoufei Wang
Seed vigor is an imperative trait for the direct seeding of rice. Isopropylmalate synthase (IPMS) catalyzes the committed step of leucine (Leu) biosynthesis, but its effect on seed vigor remains unclear. In this study, rice OsIPMS1 and OsIPMS2 was cloned, and the roles of OsIPMS1 in seed vigor were mainly investigated. OsIPMS1 and OsIPMS2 catalyze Leu biosynthesis, and Leu feedback inhibits their IPMS activities. Disruption of OsIPMS1 resulted in low seed vigor under various conditions, which might be tightly associated with the reduction of amino acids in germinating seeds...
June 27, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29905035/a-cstu-ts1-regulatory-module-promotes-fruit-tubercule-formation-in-cucumber
#12
Sen Yang, Changlong Wen, Bin Liu, Yanling Cai, Shudan Xue, Ezra S Bartholomew, Mingming Dong, Chen Jian, Shuo Xu, Ting Wang, Wenzhu Qi, Jinan Pang, Dehua Ma, Xingwang Liu, Huazhong Ren
The fruit epidermal features such as the size of tubercules are important fruit quality traits for cucumber production. But the mechanisms underlying tubercule formation remain elusive. Here, tubercule size locus CsTS1 was identified by map-based cloning, and was found to encode an oleosin protein. Allelic variation was identified in the promoter region of CsTS1, resulting in low expression of CsTS1 in all 22 different small-warty or non-warty cucumber lines. High CsTS1 expression levels were closely correlated with increased fruit tubercule size among 44 different cucumber lines...
June 14, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29890031/production-of-complex-viral-glycoproteins-in-plants-as-vaccine-immunogens
#13
REVIEW
Emmanuel Margolin, Ros Chapman, Anna-Lise Williamson, Edward P Rybicki, Ann E Meyers
Plant molecular farming offers a cost-effective and scalable approach to the expression of recombinant proteins which has been proposed as an alternative to conventional production platforms for developing countries. In recent years, numerous proofs of concept have established that plants can produce biologically active recombinant proteins and immunologically relevant vaccine antigens that are comparable to those made in conventional expression systems. Driving many of these advances is the remarkable plasticity of the plant proteome which enables extensive engineering of the host cell, as well as the development of improved expression vectors facilitating higher levels of protein production...
June 11, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29890030/qtl-sequencing-strategy-to-map-genomic-regions-associated-with-resistance-to-ascochyta-blight-in-chickpea
#14
Amit Deokar, Mandeep Sagi, Ketema Daba, Bunyamin Tar'an
Whole-genome sequencing-based bulked segregant analysis (BSA) for mapping quantitative trait loci (QTL) provides an efficient alternative approach to conventional QTL analysis as it significantly reduces the scale and cost of analysis with comparable power to QTL detection using full mapping population. We tested the application of next-generation sequencing (NGS)-based BSA approach for mapping QTLs for ascochyta blight resistance in chickpea using two recombinant inbred line populations CPR-01 and CPR-02. Eleven QTLs in CPR-01 and six QTLs in CPR-02 populations were mapped on chromosomes Ca1, Ca2, Ca4, Ca6 and Ca7...
June 11, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29878511/qqs-orphan-gene-and-its-interactor-nf-yc4-reduce-susceptibility-to-pathogens-and-pests
#15
Mingsheng Qi, Wenguang Zheng, Xuefeng Zhao, Jessica D Hohenstein, Yuba Kandel, Seth O'Conner, Yifan Wang, Chuanlong Du, Dan Nettleton, Gustavo C MacIntosh, Gregory L Tylka, Eve S Wurtele, Steven A Whitham, Ling Li
Enhancing the nutritional quality and disease resistance of crops without sacrificing productivity is a key issue for developing varieties that are valuable to farmers and for simultaneously improving food security and sustainability. Expression of the Arabidopsis thaliana species-specific AtQQS (Qua-Quine Starch) orphan gene or its interactor, NF-YC4 (Nuclear Factor Y, subunit C4), has been shown to increase levels of leaf/seed protein without affecting the growth and yield of agronomic species. Here, we demonstrate that overexpression of AtQQS and NF-YC4 in Arabidopsis and soybean enhances resistance/reduces susceptibility to viruses, bacteria, fungi, aphids and soybean cyst nematodes...
June 7, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29878497/recent-polyploidization-events-in-three-saccharum-founding-species
#16
Jisen Zhang, Qing Zhang, Leiting Li, Haibao Tang, Qiong Zhang, Yang Chen, Jie Arro, Xingtan Zhang, Aiqin Wang, Chenyong Miao, Ray Ming
The complexity of polyploid Saccharum genomes hindered progress of genome research and crop improvement in sugarcane. To understand their genome structure, transcriptomes of 59 F1 individuals derived from S. officinarum LA Purple and S. robustum Molokai 5829 (2n = 80, x = 10 for both) were sequenced, yielding 11,157 and 8,998 SNPs and 83 and 105 linkage groups, respectively. Most markers in each linkage group aligned to single sorghum chromosome. However, 71 interchromosomal rearrangements were detected between sorghum and S...
June 7, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29873883/transgenic-creeping-bentgrass-overexpressing-osa-mir393a-exhibits-altered-plant-development-and-improved-multiple-stress-tolerance
#17
Junming Zhao, Shuangrong Yuan, Man Zhou, Ning Yuan, Zhigang Li, Qian Hu, Frank G Bethea, Haibo Liu, Shigui Li, Hong Luo
MicroRNA393 (miR393) has been implicated in plant growth, development and multiple stress responses in annual species such as Arabidopsis and rice. However, the role of miR393 in perennial grasses remains unexplored. Creeping bentgrass (Agrostis stolonifera L.) is an environmentally and economically important C3 cool-season perennial turfgrass. Understanding how miR393 functions in this representative turf species would allow the development of novel strategies in genetically engineering grass species for improved abiotic stress tolerance...
June 6, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29873878/up-regulation-of-lipid-biosynthesis-increases-the-oil-content-in-leaves-of-sorghum-bicolor
#18
Thomas Vanhercke, Srinivas Belide, Matthew C Taylor, Anna El Tahchy, Shoko Okada, Vivien Rolland, Qing Liu, Madeline Mitchell, Pushkar Shrestha, Ingrid Venables, Lina Ma, Cheryl Blundell, Anu Mathew, Lisa Ziolkowski, Nathalie Niesner, Dawar Hussain, Bei Dong, Guoquan Liu, Ian D Godwin, Jiwon Lee, Melanie Rug, Xue-Rong Zhou, Surinder P Singh, James R Petrie
Synthesis and accumulation of the storage lipid triacylglycerol in vegetative plant tissues has emerged as a promising strategy to meet the world's future need for vegetable oil. Sorghum (Sorghum bicolor) is a particularly attractive target crop given its high biomass, drought resistance and C4 photosynthesis. While oilseed-like triacylglycerol levels have been engineered in the C3 model plant tobacco, progress in C4 monocot crops has been lagging behind. In this study, we report the accumulation of triacylglycerol in sorghum leaf tissues to levels between 3 and 8...
June 6, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29851301/the-developmental-dynamics-of-the-populus-stem-transcriptome
#19
Qing Chao, Zhi-Fang Gao, Dong Zhang, Biligen-Gaowa Zhao, Feng-Qin Dong, Chun-Xiang Fu, Li-Jun Liu, Bai-Chen Wang
The Populus shoot undergoes primary growth (longitudinal growth) followed by secondary growth (radial growth), which produces biomass that is an important source of energy worldwide. We adopted joint PacBio Iso-Seq and RNA-seq analysis to identify differentially expressed transcripts along a developmental gradient from the shoot apex to the fifth internode of Populus Nanlin895. We obtained 87 150 full-length transcripts, including 2081 new isoforms and 62 058 new alternatively spliced isoforms, most of which were produced by intron retention, that were used to update the Populus annotation...
May 31, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29851294/an-actin-depolymerizing-factor-from-the-halophyte-smooth-cordgrass-spartina-alterniflora-saadf2-is-superior-to-its-rice-homolog-osadf2-in-conferring-drought-and-salt-tolerance-when-constitutively-overexpressed-in-rice
#20
Sonali Sengupta, Venkata Mangu, Luis Sanchez, Renesh Bedre, Rohit Joshi, Kanniah Rajasekaran, Niranjan Baisakh
Actin-depolymerizing factors (ADFs) maintain the cellular actin network dynamics by regulating severing and disassembly of actin filaments in response to environmental cues. An ADF isolated from a monocot halophyte, Spartina alterniflora (SaADF2), imparted significantly higher level of drought and salinity tolerance when expressed in rice than its rice homologue OsADF2. SaADF2 differs from OsADF2 by a few amino acid residues, including a substitution in the regulatory phosphorylation site serine-6, which accounted for its weak interaction with OsCDPK6 (calcium-dependent protein kinase), thus resulting in an increased efficacy of SaADF2 and enhanced cellular actin dynamics...
May 31, 2018: Plant Biotechnology Journal
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