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Plant Biotechnology Journal

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https://www.readbyqxmd.com/read/28429576/suppression-of-osmads7-in-rice-endosperm-stabilizes-amylose-content-under-high-temperature-stress
#1
Hua Zhang, Heng Xu, Mengjie Feng, Ying Zhu
High temperature significantly alters the amylose content of rice, resulting in mature grains with poor eating quality. However, only few genes and/or quantitative trait loci (QTLs) involved in this process have been isolated and the molecular mechanisms of this effect remain unclear. Here, we describe a floral organ identity gene, OsMADS7, involved in stabilizing rice amylose content at high temperature. OsMADS7 is greatly induced by high temperature at the early filling stage. Constitutive suppression of OsMADS7 stabilizes amylose content under high temperature stress but results in low spikelet fertility...
April 20, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28421718/small-serine-recombination-systems-para-mrs-and-cinh-rs2-perform-precise-excision-of-plastid-dna
#2
Min Shao, Ann Blechl, James G Thomson
Selectable marker genes (SMGs) are necessary for selection of transgenic plants. However, once stable transformants have been identified, the marker gene is no longer needed. In this study, we demonstrate the use of the small serine recombination systems, ParA-MRS and CinH-RS2, to precisely excise a marker gene from the plastid genome of tobacco. Transplastomic plants transformed with the pTCH-MRS and pTCH-RS2 vectors, containing the visual reporter gene DsRed flanked by directly oriented MRS and RS2 recognition sites, respectively, were crossed with nuclear-genome transformed tobacco plants expressing plastid-targeted ParA and CinH recombinases, respectively...
April 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28421694/molecular-engineering-and-plant-expression-of-an-immunoglobulin-heavy-chain-scaffold-for-delivery-of-a-dengue-vaccine-candidate
#3
Mi-Young Kim, Craig Van Dolleweerd, Alastair Copland, Matthew John Paul, Sven Hofmann, Gina R Webster, Emily Julik, Ivonne Ceballos-Olvera, Jorge Reyes-Del Valle, Moon-Sik Yang, Yong-Suk Jang, Rajko Reljic, Julian K Ma
In order to enhance vaccine uptake by the immune cells in vivo, molecular engineering approach was employed to construct a Polymeric Immunoglobulin G Scaffold (PIGS) that incorporates multiple copies of an antigen and targets the Fc gamma receptors on antigen-presenting cells. These self-adjuvanting immunogens were tested in the context of dengue infection, for which there is currently no globally licensed vaccine yet. Thus, the consensus domain III sequence (cEDIII) of dengue glycoprotein E was incorporated into PIGS and expressed in both tobacco plants and Chinese Ovary Hamster cells...
April 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28421663/cloning-and-plant-based-production-of-antibody-mc10e7-for-a-lateral-flow-immunoassay-to-detect-4-arginine-microcystin-in-fresh-water
#4
Stanislav Melnik, A-C Neumann, R Karongo, S Dirndorfer, Martin Stübler, Verena Ibl, R Niessner, Dietmar Knopp, Eva Stoger
Antibody MC10E7 is one of a small number of monoclonal antibodies that bind specifically to [Arg4]-microcystins and it can be used to survey natural water sources and food samples for algal toxin contamination. However, the development of sensitive immunoassays in different test formats, particularly user-friendly tests for on-site analysis, requires a sensitive but also cost-effective antibody. The original version of MC10E7 was derived from a murine hybridoma, but we determined the sequence of the variable regions using the peptide mass-assisted cloning strategy and expressed a scFv (single-chain variable fragment) format of this antibody in yeast and a chimeric full size version in leaves of Nicotiana tabacum and N...
April 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28418615/co-expression-of-gr79-epsps-and-gat-yields-herbicide-resistant-cotton-with-low-glyphosate-residues
#5
Chengzhen Liang, Bao Sun, Zhigang Meng, Zhaohong Meng, Yuan Wang, Guoqing Sun, Tao Zhu, Wei Lu, Wei Zhang, Waqas Malik, Min Lin, Rui Zhang, Sandui Guo
Glyphosate resistant (GR) crops have been adopted on a massive scale by North and South American farmers. Currently, about 80% of the 120 million hectares of the global genetically-modified (GM) crops are GR crop varieties. However, the adoption of GR plants in China has not occurred at the same pace, owing to several factors including, among other things, labor markets and the residual effects of glyphosate in transgenic plants. Here, we report the co-expression of codon-optimized forms of GR79 EPSPS and N-acetyltransferase (GAT) genes in cotton...
April 18, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28419665/lettuce-produced-hepatitis-c-virus-e1e2-heterodimer-triggers-immune-responses-in-mice-and-antibody-production-after-oral-vaccination
#6
Jihong Liu Clarke, Lisa Paruch, Mihaela-Olivia Dobrica, Iuliana Caras, Catalin Tucureanu, Adrian Onu, Sonya Ciulean, Crina Stavaru, Andre Eerde, Yanliang Wang, Hege Steen, Sissel Haugslien, Catalina Petrareanu, Catalin Lazar, Ioan Popescu, Ralph Bock, Jean Dubuisson, Norica Branza-Nichita
The hepatitis C virus (HCV) is a major etiologic agent for severe liver diseases (e.g., cirrhosis, fibrosis and hepatocellular carcinoma). Approximately 140 million people have chronic HCV infections and about 500,000 die yearly from HCV-related liver pathologies. To date, there is no licenced vaccine available to prevent HCV infection and production of an HCV vaccine remains a major challenge. Here we report the successful production of the HCV E1E2 heterodimer, an important vaccine candidate, in an edible crop (lettuce, Lactuca sativa) using Agrobacterium-mediated transient expression technology...
April 17, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28403535/assembly-and-comparison-of-two-closely-related-brassica-napus-genomes
#7
Philipp E Bayer, Bhavna Hurgobin, Agnieszka A Golicz, Chon-Kit Kenneth Chan, Yuxuan Yuan, HueyTyng Lee, Michael Renton, Jinling Meng, Ruiyuan Li, Yan Long, Jun Zou, Ian Bancroft, Boulos Chalhoub, Graham J King, Jacqueline Batley, David Edwards
As an increasing number of plant genome sequences become available, it is clear that gene content varies between individuals, and the challenge arises to predict the gene content of a species. However, genome comparison is often confounded by variation in assembly and annotation. Differentiating between true gene absence and variation in assembly or annotation is essential for the accurate identification of conserved and variable genes in a species. Here we present the de novo assembly of the B. napus cultivar Tapidor and comparison with an improved assembly of the B...
April 12, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28379609/rna-guided-cas9-as-an-in-vivo-desired-target-mutator-in-maize
#8
Chuxi Li, Changlin Liu, Xiantao Qi, Yongchun Wu, Xiaohong Fei, Long Mao, Beijiu Chen, Xinhai Li, Chuanxiao Xie
The RNA-guided Cas9 system is a versatile tool for genome editing. Here, we established a RNA-guided endonuclease (RGEN) system as an in vivo desired-target mutator (DTM) in maize to reduce the linkage drag during breeding procedure, using the LIGULELESS1 (LG1) locus as a proof-of-concept. Our system showed 51.5% to 91.2% mutation frequency in T0 transgenic plants. We then crossed the T1 plants stably expressing DTM with six diverse recipient maize lines and found that 11.79% to 28.71% of the plants tested were mutants induced by the DTM effect...
April 5, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28378532/overexpression-of-an-arabidopsis-thaliana-galactinol-synthase-gene-improves-drought-tolerance-in-transgenic-rice-and-increased-grain-yield-in-the-field
#9
Michael Gomez Selvaraj, Takuma Ishizaki, Milton Valencia, Satoshi Ogawa, Beata Dedicova, Takuya Ogata, Kyouko Yoshiwara, Kyonoshin Maruyama, Miyako Kusano, Kazuki Saito, Fuminori Takahashi, Kazuo Shinozaki, Kazuo Nakashima, Manabu Ishitani
Drought stress has often caused significant decreases in crop production which could be associated with global warming. Enhancing drought tolerance without a grain yield penalty has been a great challenge in crop improvement. Here, we report the Arabidopsis thaliana galactinol synthase 2 gene (AtGolS2) was able to confer drought tolerance and increase grain yield in two different rice (Oryza sativa) genotypes under dry field conditions. The developed Ubi:AtGolS2 transgenic lines also had higher levels of galactinol than the non-transgenic control...
April 5, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28376249/elevated-auxin-and-reduced-cytokinin-contents-in-rootstocks-improve-their-performance-and-grafting-success
#10
Wei Li, Chu Fang, Sanalkumar Krishnan, Junmei Chen, Hao Yu, Angus S Murphy, Emily Merewitz, Lorenzo Katin-Grazzini, Richard J McAvoy, Ziniu Deng, Janice Zale, Yi Li
Plant grafting is an important technique for horticultural and silvicultural production. However, many rootstock plants suffer from undesirable lateral bud outgrowth, low grafting success rates, or poor rooting. Here we used a root-predominant gene promoter (SbUGT) to drive the expression of a tryptophan-2-mono oxygenase gene (iaaM) from Agrobacterium tumafaciens to increase auxin levels in tobacco. The transgenic plants, when used as a rootstock, displayed inhibited lateral bud outgrowth, enhanced grafting success rate, and improved root initiation...
April 4, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28371241/adp-glucose-pyrophosphorylase-genes-associated-with-kernel-weight-underwent-selection-during-wheat-domestication-and-breeding
#11
Jian Hou, Tian Li, Yamei Wang, Chenyang Hao, Hongxia Liu, Xueyong Zhang
ADP-glucose pyrophosphorylase (AGPase), comprising two small subunits and two large subunits, is considered a key enzyme in the endosperm starch synthesis pathway in wheat (Triticum aestivum L.). Two genes, TaAGP-S1-7A and TaAGP-L-1B, were investigated in this study. Haplotypes of these genes were associated with thousand kernel weight (TKW) in different populations. Mean TKWs of favored haplotypes were significantly higher than those of non-favored ones. Two molecular markers developed to distinguish these haplotypes could be used in molecular breeding...
April 1, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28371222/new-variants-of-crispr-rna-guided-genome-editing-enzymes
#12
REVIEW
Jana Murovec, Žan Pirc, Bing Yang
CRISPR-mediated genome editing using the Streptococcus pyogenes Cas9 enzyme is revolutionizing life science by providing new, precise, facile and high throughput tools for genetic modification by the specific targeting of double-strand breaks in the genome of hosts. Plant biotechnologists have extensively used the S. pyogenes Cas9-based system since its inception in 2013. However, there are still some limitations to its even broader usage in plants. Major restrictions, especially in agricultural biotechnology, are the currently unclear regulatory status of plants modified with CRISPR/Cas9 and the lack of suitable delivery methods for some plant species...
April 1, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28371176/silencing-of-slpl-solyc03g111690-which-encodes-a-pectate-lyase-in-tomato-confers-enhanced-fruit-firmness-prolonged-shelf-life-and-reduced-susceptibility-to-gray-mold
#13
Lu Yang, Wei Huang, Fangjie Xiong, Zhiqiang Xian, Deding Su, Maozhi Ren, Zhengguo Li
Pectate lyase genes have been documented as excellent candidates for improvement of fruit firmness. However, implementation of pectate lyase in regulating fruit postharvest deterioration has not been fully explored. In this report, 22 individual pectate lyase genes in tomato were identified, and one pectate lyase gene SlPL (Solyc03g111690), showed dominant expression during fruit maturation. RNA-interference of SlPL resulted in enhanced fruit firmness and changes in pericarp cells. More importantly, the SlPL-RNAi fruit demonstrated greater anti-rotting and pathogen-resisting ability...
April 1, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28371200/engineering-canker-resistant-plants-through-crispr-cas9-targeted-editing-of-the-susceptibility-gene-cslob1-promoter-in-citrus
#14
Aihong Peng, Shanchun Chen, Tiangang Lei, Lanzhen Xu, Yongrui He, Liu Wu, Lixiao Yao, Xiuping Zou
Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is severely damaging to the global citrus industry. Targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistance through CRISPR/Cas9-targeted modification of the susceptibility gene CsLOB1 promoter in citrus. Wanjincheng orange (Citrus sinensis Osbeck) harbors at least three copies of the CsLOB1(G) allele and one copy of the CsLOB1(-) allele...
March 29, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28371164/genome-wide-association-study-discovered-candidate-genes-of-verticillium-wilt-resistance-in-upland-cotton-gossypium-hirsutum-l
#15
Tinggang Li, Xuefeng Ma, Nanyang Li, Lei Zhou, Zheng Liu, Huanyong Han, Yuejing Gui, Yuming Bao, Jieyin Chen, Xiaofeng Dai
Verticillium wilt (VW), caused by infection by Verticillium dahliae, is considered one of the most yield-limiting diseases in cotton. To examine the genetic architecture of cotton VW resistance, we performed a genome-wide association study (GWAS) using a panel of 299 accessions and 85,630 single-nucleotide polymorphisms (SNPs) detected using the specific-locus amplified fragment sequencing (SLAF-seq) approach. Trait-SNP association analysis detected a total of 17 significant SNPs at P < 1.17 × 10(-5) (P = 1/85630, -log10 P = 4...
March 29, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28370938/mapping-of-homoeologous-chromosome-exchanges-influencing-quantitative-trait-variation-in-brassica-napus
#16
Anna Stein, Olivier Coriton, Mathieu Rousseau-Gueutin, Birgit Samans, Sarah V Schiessl, Christian Obermeier, Isobel A P Parkin, Anne-Marie Chèvre, Rod J Snowdon
Genomic rearrangements arising during polyploidization are an important source of genetic and phenotypic variation in the recent allopolyploid crop Brassica napus. Exchanges among homoeologous chromosomes, due to inter-homoeologue pairing, and deletions without compensating homoeologous duplications are observed in both natural B. napus and synthetic B. napus. Rearrangements of large or small chromosome segments induce gene copy number variation (CNV) and can potentially cause phenotypic changes. Unfortunately, complex genome restructuring is difficult to deal with in linkage mapping studies...
March 29, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28332302/protein-bodies-how-the-er-deals-with-high-accumulation-of-recombinant-proteins
#17
Reza Saberianfar, Rima Menassa
Protein bodies (PBs) are highly specialized protein storage organelles in cereal seeds. PB formation in seeds initiates in the endoplasmic reticulum (ER), and depending on the plant species, PBs remain in the ER or find their way out of the ER, bypass the Golgi and end up in protein storage vacuoles (PSVs) (Khan et al., 2012). Protein bodies have been ectopically induced in leaves by producing high amounts of ER-retrieved recombinant proteins, usually by fusing them to protein tags such as Zera(®) , elastin-like polypeptide (ELP) and hydrophobin-I (HFBI)...
March 23, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28332274/time-course-expression-qtl-atlas-of-the-global-transcriptional-response-of-wheat-to-fusarium-graminearum
#18
Mina Samad-Zamini, Wolfgang Schweiger, Thomas Nussbaumer, Klaus F X Mayer, Hermann Buerstmayr
Fusarium head blight is a devastating disease of small grain cereals such as bread wheat (Triticum aestivum). The pathogen switches from a biotrophic to a nectrotrophic lifestyle in course of disease development forcing its host to adapt its defence strategies. Using a genetical genomics approach we illustrate genome-wide reconfigurations of genetic control over transcript abundances between two decisive time points after inoculation with the causative pathogen Fusarium graminearum. Whole transcriptome measurements have been recorded for 163 lines of a wheat doubled haploid population segregating for several resistance genes yielding 15 552 at 30 hours and 15 888 eQTL at 50 hours after inoculation...
March 23, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28317293/a-new-location-to-split-cre-recombinase-for-protein-fragment-complementation
#19
Maryam Rajaee, David W Ow
We have previously described a recombinase-mediated gene stacking system in which the Cre recombinase is used to remove lox-site flanked DNA no longer needed after each round of Bxb1 integrase-mediated site-specific integration. The Cre recombinase can be conveniently introduced by hybridization with a cre-expressing plant. However, maintaining an efficient cre-expressing line over many generations can be a problem, as high production of this DNA binding protein might interfere with normal chromosome activities...
March 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28317264/a-collection-of-enhancer-trap-insertional-mutants-for-functional-genomics-in-tomato
#20
Fernando Pérez-Martín, Fernando J Yuste-Lisbona, Benito Pineda, María Pilar Angarita-Díaz, Begoña García-Sogo, Teresa Antón, Sibilla Sánchez, Estela Giménez, Alejandro Atarés, Antonia Fernández-Lozano, Ana Ortíz-Atienza, Manuel García-Alcázar, Laura Castañeda, Rocío Fonseca, Carmen Capel, Geraldine Goergen, Jorge Sánchez, Jorge L Quispe, Juan Capel, Trinidad Angosto, Vicente Moreno, Rafael Lozano
With the completion of genome sequencing projects, the next challenge is to close the gap between gene annotation and gene functional assignment. Genomic tools to identify gene functions are based on the analysis of phenotypic variations between a wild type and its mutant; hence, mutant collections are a valuable resource. In this sense, T-DNA collections allow for an easy and straightforward identification of the tagged gene, serving as the basis of both forward and reverse genetic strategies. This study reports on the phenotypic and molecular characterization of an enhancer trap T-DNA collection in tomato (Solanum lycopersicum L...
March 19, 2017: Plant Biotechnology Journal
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