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Plant Biotechnology Journal

E M Ramos-Martinez, L Fimognari, Y Sakuragi
Microalga-based biomanufacturing of recombinant proteins is attracting growing attention due to its advantages in safety, metabolic diversity, scalability, and sustainability. Secretion of recombinant proteins can accelerate the use of microalgal platforms by allowing post-translational modifications and easy recovery of products from the culture media. However, currently, the yields of secreted recombinant proteins are low, which hampers the commercial application of this strategy. This study aimed at expanding the genetic tools for enhancing secretion of recombinant proteins in Chlamydomonas reinhardtii, a widely used green microalga as a model organism and a potential industrial biotechnology platform...
February 16, 2017: Plant Biotechnology Journal
Mi Ni, Wei Ma, Xiaofang Wang, Meijing Gao, Yan Dai, Xiaoli Wei, Lei Zhang, Yonggang Peng, Shuyuan Chen, Lingyun Ding, Yue Tian, Jie Li, Haiping Wang, Xiaolin Wang, Guowang Xu, Wangzhen Guo, Yihua Yang, Yidong Wu, Shannon Heuberger, Bruce E Tabashnik, Tianzhen Zhang, Zhen Zhu
Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant "pyramids" producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross-resistance and antagonism between Bt toxins limits the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi)...
February 15, 2017: Plant Biotechnology Journal
Kongqing Li, Caihua Xing, Zhenghong Yao, Xiaosan Huang
MYB comprises a large family of transcription factors that play significant roles in plants development and stress response in plants. However, knowledge concerning the functions of MYBs and the target genes remain poorly understood. Here, we report the identification and functional characterization of a novel stress-responsive MYB gene from Pyrus betulaefolia. The MYB gene, designated as PbrMYB21, belongs to the R2R3-type and shares high degree of sequence similarity to MdMYB21. The transcript levels of PbrMYB21 were up-regulated under various abiotic stresses, particularly dehydration...
February 11, 2017: Plant Biotechnology Journal
Junfang Kang, Jianmin Li, Shuang Gao, Chao Tian, Xiaojun Zha
Drought represents a key limiting factor of global crop distribution. Receptor-like kinases play major roles in plant development and defense responses against stresses such as drought. In this study, LRK2, which encodes a leucine-rich receptor-like kinase, was cloned and characterized and found to be localized on the plasma membrane in rice. Promoter-GUS analysis revealed strong expression in tiller buds, roots, nodes and anthers. Transgenic plants overexpressing LRK2 exhibited enhanced tolerance to drought stress due to an increased number of lateral roots compared to the wild-type at the vegetative stage...
February 9, 2017: Plant Biotechnology Journal
Vrijesh Kumar Yadav, Vikash Kumar Yadav, Poonam Pant, Surendra Pratap Singh, Rashmi Maurya, Anshulika Sable, Samir V Sawant
Secondary cell wall (SCW) biosynthesis is an important stage of the cotton fiber development, its transcriptional regulation is poorly understood. We selected the Gossypium hirsutum GDSL (GhGDSL) lipase/hydrolase gene (CotAD_74480), which is expressed during SCW biosynthesis (19 through to 25 Days Post Anthesis; DPA), for study. T1 -transgenic cotton lines expressing the β-glucuronidase (gus) reporter under the control of a 1026-bp promoter fragment of GhGDSL (PGhGDSL ) showed 19 DPA stage-specific increase in GUS expression...
February 9, 2017: Plant Biotechnology Journal
Jun-Jun Liu, Richard A Sniezko, Arezoo Zamany, Holly Williams, Ning Wang, Angelia Kegley, Douglas P Savin, Hao Chen, Rona N Sturrock
Molecular breeding incorporates efficient tools to increase rust resistance in five-needle pines. Susceptibility of native five-needle pines to white pine blister rust (WPBR), caused by the non-native invasive fungus Cronartium ribicola (J.C.Fisch.), has significantly reduced wild populations of these conifers in North America. Major resistance (R) genes against specific avirulent pathotypes have been found in several five-needle pine species. In this study we screened genic SNP markers by comparative transcriptome and genetic association analyses and constructed saturated linkage maps for the western white pine (Pinus monticola) R locus (Cr2)...
February 7, 2017: Plant Biotechnology Journal
Junliang Zhao, Shaohong Zhang, Jingfang Dong, Tifeng Yang, Xingxue Mao, Qing Liu, Xiaofei Wang, Bin Liu
Identification and cloning of cold-tolerant genes that can stably express under different cold environments are crucial for molecular rice breeding for cold tolerance. In the previous study, we identified a cold-tolerant QTL at the seedling stage, qCTS-9 which could be detected under different cold environments using a recombinant inbred line (RIL) population derived from a cold-tolerant variety Lijiangxintuanheigu (LTH) and a cold-sensitive variety Shanhuangzhan 2 (SHZ-2). In this study, eight candidate genes within the qCTS-9 interval were identified through integrated analysis of QTL mapping with genome-wide differential expression profiling of LTH...
February 7, 2017: Plant Biotechnology Journal
Laura Stoffels, Henry N Taunt, Bambos Charalambous, Saul Purton
There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage-infected cells to digest the bacterial cell wall for phage progeny release at the end of the lytic cycle. These highly efficient enzymes show a considerable degree of specificity for the target bacterium of the phage. Furthermore, the emergence of resistance against endolysins appears to be rare as the enzymes have evolved to target molecules in the cell wall that are essential for bacterial viability...
February 3, 2017: Plant Biotechnology Journal
Vaishnavi Amarr Reddy, Qian Wang, Niha Dhar, Nadimuthu Kumar, Prasanna Nori Venkatesh, Chakravarthy Rajan, Deepa Panicker, Vishweshwaran Sridhar, Hui-Zhu Mao, Rajani Sarojam
Many aromatic plants, like spearmint, produce valuable essential oils in specialized structures called peltate glandular trichomes (PGT). Understanding the regulatory mechanisms behind the production of these important secondary metabolites will help design new approaches to engineer them. Here, we identified a PGT-specific R2R3-MYB gene MsMYB, from comparative RNA-Seq data of spearmint, and functionally characterized it. Analysis of MsMYB-RNAi transgenic lines showed increased levels of monoterpenes and MsMYB-overexpressing lines exhibited decreased levels of monoterpenes...
February 3, 2017: Plant Biotechnology Journal
Uri Hanania, Tami Ariel, Yoram Tekoah, Liat Fux, Maor Sheva, Yehuda Gubbay, Mara Weiss, Dina Oz, Yaniv Azulay, Albina Turbovski, Yehava Forster, Yoseph Shaaltiel
Plant produced glycoproteins contain N-linked glycans with plant-specific residues of β(1,2)-xylose and core α(1,3)-fucose, which do not exist in mammalian derived proteins. Although our experience with two enzymes that are used for enzyme replacement therapy (ERT) does not indicate that the plant sugar residues have deleterious effects, we made a conscious decision to eliminate these moieties from plant-expressed proteins. We knocked out the β(1,2)-xylosyltranferase (XylT) and the α(1,3)-fucosyltransferase (FucT) genes, using CRISPR/Cas9 genome editing, in Nicotiana...
February 3, 2017: Plant Biotechnology Journal
Fengcheng Li, Guosheng Xie, Jiangfeng Huang, Ran Zhang, Yu Li, Miaomiao Zhang, Yanting Wang, Ao Li, Xukai Li, Tao Xia, Chengcheng Qu, Fan Hu, Arthur J Ragauskas, Liangcai Peng
Genetic modification of plant cell walls has been posed to reduce lignocellulose recalcitrance for enhancing biomass saccharification. Since cellulose synthase (CESA) gene is first identified, several dozen CESA mutants have been reported, but almost all mutants exhibit the defective phenotypes in plant growth and development. In this study, the rice (Oryza sativa) Osfc16 mutant with substitutions (W481C, P482S) at P-CR conserved-site in CESA9 shows a slightly affected plant growth and higher biomass yield by 25%-41% compared with wild type (Nipponbare, a japonica variety)...
January 24, 2017: Plant Biotechnology Journal
Poonam Mehra, Bipin Kumar Pandey, Jitender Giri
Phosphate (Pi) deficiency in soil system is a limiting factor for rice growth and yield. Majority of the soil Phosphorus (P) is organic in nature; not readily available for root uptake. Low Pi inducible purple acid phosphatases (PAPs) are hypothesized to enhance the availability of Pi in soil and cellular system. However, information on molecular and physiological roles of rice PAPs is very limited. Here, we demonstrate the role of a novel rice PAP, OsPAP21b in improving plant utilization of organic-P. OsPAP21b was found to be under the transcriptional control of OsPHR2 and strictly regulated by plant Pi-status at both transcript and protein levels...
January 24, 2017: Plant Biotechnology Journal
Cong Dang, Zengbin Lu, Long Wang, Xuefei Chang, Fang Wang, Hongwei Yao, Yufa Peng, David Stanley, Gongyin Ye
Transgenic Bt rice expressing the insecticidal proteins derived from Bacillus thuringiensis Berliner (Bt) have been developed since 1989. Their ecological risks toward non-target organisms have been investigated, however, these studies were conducted individually, yielding uncertainty regarding potential agroecological risks associated with large-scale deployment of Bt rice lines. Here, we developed a meta-analysis of existing literature to synthesize current knowledge of the impacts of Bt rice on functional arthropod guilds, including herbivores, predators, parasitoids and detritivores in laboratory and field studies...
January 23, 2017: Plant Biotechnology Journal
Parwinder Kaur, Philipp E Bayer, Zbyněk Milec, Jan Vrána, Yuxuan Yuan, Rudi Appels, David Edwards, Jacqueline Batley, Phillip Nichols, William Erskine, Jaroslav Doležel
Subterranean clover is an important annual forage legume, whose diploidy and inbreeding nature make it an ideal model for genomic analysis in Trifolium. We reported a draft genome assembly of the subterranean clover TSUd_r1.1. Here we evaluate genome mapping on nanochannel arrays and generation of a transcriptome atlas across tissues to advance the assembly and gene annotation. Using a BioNano-based assembly spanning 512 Mb (93% genome coverage), we validated the draft assembly, anchored unplaced contigs and resolved misassemblies...
January 23, 2017: Plant Biotechnology Journal
Jie-Li Wang, Min-Qiang Tang, Sheng Chen, Xiang-Feng Zheng, Hui-Xian Mo, Sheng-Jun Li, Zheng Wang, Ke-Ming Zhu, Li-Na Ding, Sheng-Yi Liu, Yun-Hai Li, Xiao-Li Tan
Brassica napus L. is an important oil crop worldwide, and is the main raw material for biofuel. Seed weight and seed size are the main contributors to seed yield. DA1 (DA means big in Chinese) is an ubiquitin receptor and negatively regulates seed size. Down-regulation of AtDA1 in Arabidopsis leads to larger seeds and organs by increasing cell proliferation in integuments. In this study, BnDA1was down-regulated in B. napus by over expressed of AtDA1(R358K) , which is a functional deficiency of DA1 with an arginine-to-lysine mutation at the 358(th) amino acid...
January 17, 2017: Plant Biotechnology Journal
Olga Yurchenko, Jay M Shockey, Satinder K Gidda, Maxwell I Silver, Kent D Chapman, Robert T Mullen, John M Dyer
The seeds of many non-domesticated plant species synthesize oils containing high amounts of a single unusual fatty acid, many of which have potential usage in industry. Despite the identification of enzymes for unusual oxidized fatty acid synthesis, the production of these fatty acids in engineered seeds remains low and is often hampered by their inefficient exclusion from phospholipids. Recent studies have established the feasibility of increasing triacylglycerol content in plant leaves, which provides a novel approach for increasing energy density of biomass crops...
January 13, 2017: Plant Biotechnology Journal
Thuanne Pires Ribeiro, Fabricio Barbosa Monteiro Arraes, Isabela Tristan Lourenço-Tessutti, Marilia Santos Silva, Maria Eugênia Lisei-de-Sá, Wagner Alexandre Lucena, Leonardo Lima Pepino Macedo, Janaina Nascimento Lima, Regina Maria Santos Amorim, Sinara Artico, Márcio Alves-Ferreira, Maria Cristina Mattar Silva, Maria Fatima Grossi-de-Sa
Genetically modified (GM) cotton events that effectively control cotton boll weevil (CBW), which is the most destructive cotton insect pest in South America, are reported here for the first time. This work presents the successful development of new GM cotton with high resistance to CBW conferred by Cry10Aa toxin, a protein encoded by entomopathogenic Bacillus thuringiensis (Bt) gene. The plant transformation vector harbouring cry10Aa gene driven by the cotton ubiquitination-related promoter uceA1.7 was introduced into a Brazilian cotton cultivar by biolistic transformation...
January 12, 2017: Plant Biotechnology Journal
Zhengwen Sun, Xingfen Wang, Zhengwen Liu, Qishen Gu, Yan Zhang, Zhikun Li, Huifeng Ke, Jun Yang, Jinhua Wu, Liqiang Wu, Guiyin Zhang, Caiying Zhang, Zhiying Ma
Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping and genetic variation analyses of 719 diverse accessions of upland cotton based on multiple environment tests and a recently developed Cotton 63K Illumina Infinium SNP array and performed a genome-wide association study (GWAS) of fibre quality traits...
January 8, 2017: Plant Biotechnology Journal
Da Lu, Xianghe Yuan, Sung-Jin Kim, Joaquim V Marques, P Pawan Chakravarthy, Syed G A Moinuddin, Randi Luchterhand, Barri Herman, Laurence B Davin, Norman G Lewis
A foundational study assessed effects of biochemical pathway introduction into poplar to produce eugenol, chavicol, p-anol, isoeugenol, and their sequestered storage products, from potentially available substrates, coniferyl and p-coumaryl alcohols. At the onset, it was unknown whether significant carbon flux to monolignols vs other phenylpropanoid (acetate) pathway metabolites would be kinetically favored. Various transgenic poplar lines generated eugenol and chavicol glucosides in ca. 0.45% (~0.35 and ~0...
January 8, 2017: Plant Biotechnology Journal
Congwei Sun, Fuyan Zhang, Xuefang Yan, Xiangfen Zhang, Zhongdong Dong, Dangqun Cui, Feng Chen
Bread wheat is a leading cereal crop worldwide. Limited amount of superior allele loci restricted the progress of molecular improvement in wheat breeding. Here we revealed new allelic variation distribution for 13 yield-related traits in series of genome-wide association studies (GWAS) using the Wheat 90K genotyping assay, characterized in 163 bread wheat cultivars. Agronomic traits were investigated in 14 environments at three locations over three years. After filtering SNP datasets, GWAS using 20,689 high-quality SNPs associated 1,769 significant loci that explained, on average, ~20% of the phenotypic variation, both detected already reported loci and new promising genomic regions...
January 5, 2017: Plant Biotechnology Journal
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