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Plant Biotechnology Journal

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https://www.readbyqxmd.com/read/29223138/plant-expressed-fc-fusion-protein-tetravalent-dengue-vaccine-with-inherent-adjuvant-properties
#1
Mi Young Kim, Alastair Copland, Kaustuv Nayak, Anmol Chandele, Muhammad Shamsher Ahmed, Qibo Zhang, Gil Reynolds Diogo, Matthew John Paul, Sven Hofmann, Moon-Sik Yang, Yong-Suk Jang, Julian K-C Ma, Rajko Reljic
Dengue is a major global disease requiring improved treatment and prevention strategies. The recently licensed Sanofi-Pasteur Denvaxia vaccine does not protect children under the age of nine and additional vaccine strategies are thus needed to halt this expanding global epidemic. Here, we employed a molecular engineering approach and plant-expression to produce a humanised and highly immunogenic Poly-Immunoglobulin G Scaffold (PIGS) fused to the consensus dengue envelope protein III domain (cEDIII). The immunogenicity of this IgG Fc receptor targeted vaccine candidate was demonstrated in transgenic mice expressing human FcγRI/CD64, by induction of neutralising antibodies and evidence of cell-mediated immunity...
December 9, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29223136/allele-exchange-at-the-epsps-locus-confers-glyphosate-tolerance-in-cassava
#2
Aaron W Hummel, Raj Deepika Chauhan, Tomas Cermak, Andrew M Mutka, Anupama Vijayaraghavan, Adam Boyher, Colby G Starker, Rebecca Bart, Daniel F Voytas, Nigel J Taylor
Effective weed control can protect yields of cassava (Manihot esculenta) storage roots (Doll and Piedrahita Cañola, 1978). Farmers could benefit from using herbicide with a tolerant cultivar. We applied traditional transgenesis and gene editing to generate robust glyphosate tolerance in cassava. By comparing promoters regulating expression of transformed 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) genes with various paired amino acid substitutions, we found that strong constitutive expression is required to achieve glyphosate tolerance during in vitro selection and in whole cassava plants...
December 9, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29205771/homoeologous-exchange-is-a-major-cause-of-gene-presence-absence-variation-in-the-amphidiploid-brassica-napus
#3
Bhavna Hurgobin, Agnieszka A Golicz, Philipp E Bayer, Chon-Kit Kenneth Chan, Soodeh Tirnaz, Aria Dolatabadian, Sarah V Schiessl, Birgit Samans, Juan D Montenegro, Isobel A P Parkin, J Chris Pires, Boulos Chalhoub, Graham J King, Rod Snowdon, Jacqueline Batley, David Edwards
Homoeologous exchanges (HEs) have been shown to generate novel gene combinations and phenotypes in a range of polyploid species. Gene presence/absence variation (PAV) is also a major contributor to genetic diversity. In the present study we show that there is an association between these two events, particularly in recent Brassica napus synthetic accessions, and that these represent a novel source of genetic diversity, which can be captured for the improvement of this important crop species. By assembling the pangenome of B...
December 4, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29193733/overexpression-of-the-class-i-homeodomain-transcription-factor-tahdzipi-5-increases-drought-and-frost-tolerance-in-transgenic-wheat
#4
Yunfei Yang, Sukanya Luang, John Harris, Matteo Riboni, Yuan Li, Natalia Bazanova, Maria Hrmova, Stephan Haefele, Nataliya Kovalchuk, Sergiy Lopato
Characterisation of the function of stress-related genes helps to define mechanisms of plant responses to environmental conditions. The findings of this work defined the role of the wheat TaHDZipI-5 gene, encoding a stress-responsive homeodomain-leucine zipper class I (HD-Zip I) transcription factor, during the development of plant tolerance to frost and drought. Strong induction of TaHDZipI-5 expression by low temperatures, and the elevated TaHDZipI-5 levels of expression in flowers and early developing grains in the absence of stress, suggest that TaHDZipI-5 is involved in the regulation of frost tolerance at flowering...
November 28, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29193665/provitamin-a-biofortification-of-cassava-enhances-shelf-life-but-reduces-dry-matter-content-of-storage-roots-due-to-altered-carbon-partitioning-into-starch
#5
Getu Beyene, Felix R Solomon, Raj Deepika Chauhan, Eliana Gaitán-Solis, Narayanan Narayanan, Jackson Gehan, Dimuth Siritunga, Robyn L Stevens, John Jifon, Joyce Van Eck, Edward Linsler, Malia Gehan, Muhammad Ilyas, Martin Fregene, Richard T Sayre, Paul Anderson, Nigel J Taylor, Edgar B Cahoon
Storage roots of cassava, a major subsistence crop of sub-Saharan Africa, are calorie-rich but deficient in essential micronutrients, including provitamin A β-carotene. In this study, β-carotene concentrations in cassava storage roots were enhanced by co-expression of transgenes for deoxy-D-xylulose-5-phosphate synthase (DXS) and bacterial phytoene synthase (crtB), mediated by the patatin type-1 promoter. Storage roots harvested from field-grown plants accumulated carotenoids to ≤50 μg/g DW, 15- to 20-fold increases relative to roots from non-transgenic plants...
November 28, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29193664/genome-wide-discovery-of-tissue-specific-mirnas-in-clusterbean-cyamopsis-tetragonoloba-indicates-their-association-with-galactomannan-biosynthesis
#6
Anshika Tyagi, Deepti Nigam, S V Amitha Mithra, Amolkumar U Solanke, Nagendra Kumar Singh, Tilak Raj Sharma, Kishor Gaikwad
Owing to the presence of 80% soluble dietary fiber, high protein content and high value gum, clusterbean (Cyamopsis tetragonoloba) has recently emerged as an economically important legume. The developing clusterbean seeds accumulate 90% galactomannans in the endosperm, and therefore, can be used as a model crop to understand galactomannan biosynthesis and its regulation. miRNAs are tiny master regulators of their corresponding target genes, resulting in variations in the amounts of their metabolic end products...
November 28, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29193661/the-c2h2-zinc-finger-protein-slzf3-regulates-asa-synthesis-and-salt-tolerance-by-interacting-with-csn5b
#7
Ying Li, Zhuannan Chu, Jinying Luo, Yuhong Zhou, Yujing Cai, Yongen Lu, Junhui Xia, Hanhui Kuang, Zhibiao Ye, Bo Ouyang
Abiotic stresses are a major cause of crop loss. Ascorbic acid (AsA) promotes stress tolerance by scavenging reactive oxygen species (ROS), which accumulate when plants experience abiotic stress. Although the biosynthesis and metabolism of AsA are well established, the genes that regulate these pathways remain largely unexplored. Here, we report on a novel regulatory gene from tomato (Solanum lycopersicum) named SlZF3 that encodes a Cys2/His2-type zinc-finger protein with an EAR repression domain. The expression of SlZF3 was rapidly induced by NaCl treatments...
November 28, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29193541/nitrogen-use-efficiency-is-regulated-by-interacting-proteins-relevant-to-development-in-wheat
#8
Lei Lei, Genqiao Li, Hailin Zhang, Carol Powers, Tilin Fang, Yihua Chen, Shuwen Wang, Xinkai Zhu, Brett F Carver, Liuling Yan
Wheat (Triticum aestivum) has low nitrogen use efficiency (NUE). The genetic mechanisms controlling NUE are unknown. Positional cloning of a major quantitative trait locus (QTL) for N-related agronomic traits showed that the vernalization gene TaVRN-A1 was tightly linked with TaNUE1, the gene shown to influence NUE in wheat. Because of an Ala180 /Val180 substitution, TaVRN-A1a and TaVRN-A1b proteins interact differentially with TaANR1, a protein encoded by a wheat orthologue of Arabidopsis nitrate regulated 1 (ANR1)...
November 28, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29149461/long-non-coding-rnas-involve-in-resistance-to-verticillium-dahliae-a-fungal-disease-in-cotton
#9
Lin Zhang, Maojun Wang, Nannan Li, Honglei Wang, Ping Qiu, Liuling Pei, Zheng Xu, Tianyi Wang, Erlin Gao, Junxia Liu, Shiming Liu, Qin Hu, Yuhuan Miao, Keith Lindsey, Lili Tu, Longfu Zhu, Xianlong Zhang
Long non-coding RNAs (lncRNAs) have several known functions in plant development, but their possible roles in responding to plant disease remain largely unresolved. In this study, we described a comprehensive disease-responding lncRNA profiles in defense against a cotton fungal disease Verticillium dahliae. We further revealed the conserved and specific characters of disease responding process between two cotton species. Conservatively for two cotton species, we found the expression dominance of induced lncRNAs in the Dt subgenome, indicating a biased induction pattern in the co-existing subgenomes of allotetraploid cotton...
November 17, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29112324/de-novo-assembly-of-the-zucchini-genome-reveals-a-whole-genome-duplication-associated-with-the-origin-of-the-cucurbita-genus
#10
Javier Montero-Pau, José Blanca, Aureliano Bombarely, Peio Ziarsolo, Cristina Esteras, Carlos Martí-Gómez, María Ferriol, Pedro Gómez, Manuel Jamilena, Lukas Mueller, Belén Picó, Joaquín Cañizares
The Cucurbita genus (squashes, pumpkins and gourds) includes important domesticated species such as C. pepo, C. maxima and C. moschata. In this study, we present a high-quality draft of the zucchini (C. pepo) genome. The assembly has a size of 263 Mb, a scaffold N50 of 1.8 Mb, and 34,240 gene models. It includes 92% of the conserved BUSCO core gene set, and it is estimated to cover 93.0% of the genome. The genome is organized in 20 pseudomolecules that represent 81.4% of the assembly, and it is integrated with a genetic map of 7,718 SNPs...
November 7, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29106782/expression-and-assembly-of-largest-foreign-protein-in-chloroplasts-oral-delivery-of-human-fviii-made-in-lettuce-chloroplasts-robustly-suppresses-inhibitor-formation-in-hemophilia-a-mice
#11
Kwang-Chul Kwon, Alexandra Sherman, Wan-Jung Chang, Aditya Kamesh, Moanaro Biswas, Roland W Herzog, Henry Daniell
Inhibitor formation is a serious complication of factor VIII (FVIII) replacement therapy for the X-linked bleeding disorder hemophilia A and occurs in 20-30% of patients. No prophylactic tolerance protocol currently exists. Although we reported oral tolerance induction using FVIII domains expressed in tobacco chloroplasts, significant challenges in clinical advancement include expression of the full-length CTB-FVIII sequence to cover the entire patient population, regardless of individual CD4(+) T cell epitope responses...
November 6, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29105251/isolation-of-five-rice-non-endosperm-tissue-expressed-promoters-and-evaluation-of-their-activities-in-transgenic-rice
#12
Hao Li, Juan Li, Rongfang Xu, Ruiying Qin, Fengshun Song, Li Li, Pengcheng Wei, Jianbo Yang
Using promoters expressed in non-endosperm tissues to activate target genes in specific plant tissues or organs with very limited expression in the endosperm is an attractive approach in crop transgenic engineering. In this paper, five putative non-endosperm tissue expressed promoters were cloned from the rice genome and designated POsNETE1 , POsNETE2 , POsNETE3 , POsNETE4 and POsNETE5 . By qualitatively and quantitatively examining GUSplus reporter gene expression in transgenic rice plants, POsNETE1 -POsNETE5 were all found to be active in the roots, leaves, stems, sheaths and panicles but not in the endosperm of plants at different developmental stages...
November 3, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29087016/recent-insights-in-cotton-functional-genomics-progress-and-future-perspectives
#13
REVIEW
Javaria Ashraf, Zuo Dongyun, Wang Qiaolian, Waqas Malik, Zhang Youping, Muhammad Ali Abid, Cheng Hailiang, Yang Qiuhong, Song Guoli
Functional genomics has transformed from futuristic concept to well established scientific discipline during last decade. Cotton functional genomics promise to enhance the understanding of fundamental plant biology to systematically exploit genetic resources for improvement of cotton fiber quality and yield, as well as utilization of genetic information for germplasm improvement. However, determining the cotton gene functions is a much more challenging task which has not progressed at rapid pace. This article presents a comprehensive overview on the recent tools and resources available with the major advances in cotton functional genomics to develop elite cotton genotypes...
October 31, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29087011/crispr-cas9-and-talens-generate-heritable-mutations-for-genes-involved-in-small-rna-processing-of-glycine-max-and-medicago-truncatula
#14
Shaun J Curtin, Yer Xiong, Jean-Michel Michno, Benjamin W Campbell, Adrian O Stec, Tomas Čermák, Colby Starker, Daniel F Voytas, Andrew L Eamens, Robert M Stupar
Processing of double-stranded RNA precursors into small RNAs is an essential regulator of gene expression in plant development and stress response. Small RNA processing requires the combined activity of a functionally diverse group of molecular components. However, in most plant species, there are insufficient mutant resources to functionally characterize each encoding gene. Here, mutations in loci encoding protein machinery involved in small RNA processing in soybean and Medicago truncatula were generated using the CRISPR/Cas9 and TAL-effector nuclease (TALEN) mutagenesis platforms...
October 31, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29069530/agronomic-and-chemical-performance-of-field-grown-tobacco-engineered-for-triterpene-and-methylated-triterpene-metabolism
#15
Zuodong Jiang, Chase Kempinski, Santosh Kumar, Scott Kinison, Kristin Linscott, Eric Nybo, Sarah Janze, Connie Wood, Joe Chappell
Squalene is a linear intermediate to nearly all classes of triterpenes and sterols and is itself highly valued for its use in wide range of industrial applications. Another unique linear triterpene is botryococcene and its methylated derivatives generated by the alga Botryococcus braunii race B, which are progenitors to fossil fuel deposits. Production of these linear triterpenes was previously engineered into transgenic tobacco by introducing the key steps of triterpene metabolism into the particular subcellular compartments...
October 25, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29055111/genetic-based-dissection-of-arsenic-accumulation-in-maize-using-a-genome-wide-association-analysis-method
#16
Zhan Zhao, Huaisheng Zhang, Zhongjun Fu, Hao Chen, Yanan Lin, Pengshuai Yan, Weihua Li, Huiling Xie, Zhanyong Guo, Xuehai Zhang, Jihua Tang
Understanding the mechanism of arsenic (As) accumulation in plants is important in reducing As's toxicity to plants and its potential risks to human health. Here, we performed a genome-wide association study to dissect the genetic basis of the As contents of different maize tissues in Xixian, which was irrigated with As-rich surface water, and Changge using an association population consisting of 230 representative inbred maize lines. Phenotypic data revealed a wide normal distribution and high repeatability for the As contents in maize tissues...
October 21, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29055088/the-transcriptome-extracellular-proteome-and-active-secretome-of-agroinfiltrated-n-benthamiana-uncover-a-large-diverse-protease-repertoire
#17
Friederike Grosse-Holz, Steven Kelly, Svenja Blaskowski, Farnusch Kaschani, Markus Kaiser, Renier A L van der Hoorn
Infiltration of disarmed Agrobacterium tumefaciens into leaves of Nicotiana benthamiana (agroinfiltration) facilitates quick and safe production of antibodies, vaccines, enzymes and metabolites for industrial use (Molecular Farming). However, yield and purity of proteins produced by agroinfiltration are hampered by unintended proteolysis, restricting industrial viability of the agroinfiltration platform. Proteolysis may be linked to an immune response to agroinfiltration, but understanding of the response to agroinfiltration is limited...
October 21, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29055073/ppnac1-a-main-regulator-of-phenylalanine-biosynthesis-and-utilization-in-maritime-pine
#18
Mª Belén Pascual, María-Teresa Llebrés, Blanca Craven-Bartle, Rafael A Cañas, Francisco M Cánovas, Concepción Ávila
The transcriptional regulation of phenylalanine metabolism is particularly important in conifers, long-lived species that use large amounts of carbon in wood. Here, we show that the Pinus pinaster transcription factor, PpNAC1, is a main regulator of phenylalanine biosynthesis and utilization. A phylogenetic analysis classified PpNAC1 in the NST proteins group, and was selected for functional characterization. PpNAC1 is predominantly expressed in the secondary xylem and compression wood of adult trees. Silencing of PpNAC1 in P...
October 21, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29044890/overexpression-of-serine-acetyltransferase-in-maize-leaves-increases-seed-specific-methionine-rich-zeins
#19
Xiaoli Xiang, Yongrui Wu, José Planta, Joachim Messing, Thomas Leustek
Maize kernels do not contain enough of the essential sulfur-amino acid methionine (Met) to serve as a complete diet for animals, even though maize has the genetic capacity to store Met in kernels. Prior studies indicated that the availability of the sulfur (S)-amino acids may limit their incorporation into seed storage proteins. Serine acetyltransferase (SAT) is a key control point for S-assimilation leading to Cys and Met biosynthesis and SAT overexpression is known to enhance S-assimilation without negative impact on plant growth...
October 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29024337/-two-florets-spikelet-as-a-novel-resource-has-the-potential-to-increase-rice-yield
#20
Deyong Ren, Haiping Yu, Yuchun Rao, Qiankun Xu, Tingting Zhou, Jiang Hu, Yu Zhang, Guangheng Zhang, Li Zhu, Zhenyu Gao, Guang Chen, Longbiao Guo, Dali Zeng, Qian Qian
Yield in rice (Oryza sativa) is determined by three major components: panicle number per plant, grain weight, and grain/spikelet number per panicle (Zhou et al., 2015). Grain number per panicle is one of the main targets, and mainly results from the number of spikelets. Traditionally, rice breeders have focused on the improvement of spikelet number per panicle and rarely focused on the number of florets because a normal rice spikelet has one fertile floret and produces one seed. This article is protected by copyright...
October 11, 2017: Plant Biotechnology Journal
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