Read by QxMD icon Read

Plant Biotechnology Journal

Michaela McGinn, Winthrop B Phippen, Ratan Chopra, Sunil Bansal, Brice A Jarvis, Mary E Phippen, Kevin M Dorn, Maliheh Esfahanian, Tara J Nazarenus, Edgar B Cahoon, Timothy P Durrett, M David Marks, John C Sedbrook
Thlapsi arvense L. (pennycress) is being developed as a profitable oilseed cover crop for the winter fallow period throughout temperate regions of the world, controlling soil erosion and nutrients runoff on otherwise barren farmland. We demonstrate that pennycress can serve as a user-friendly model system akin to Arabidopsis that is well-suited for both laboratory and field experimentation. We sequenced the diploid genome of the spring-type Spring 32-10 inbred line (1C DNA content of 539 Mb; 2n=14), identifying variation that may explain phenotypic differences with winter-type pennycress, as well as predominantly a one-to-one correspondence with Arabidopsis genes, which makes translational research straightforward...
September 19, 2018: Plant Biotechnology Journal
Philipp E Bayer, Agnieszka A Golicz, Soodeh Tirnaz, Chon-Kit Kenneth Chan, David Edwards, Jacqueline Batley
Brassica oleracea is an important agricultural species encompassing many vegetable crops including cabbage, cauliflower, broccoli and kale, however it can be susceptible to a variety of fungal diseases such as clubroot, blackleg, leaf spot, and downy mildew. Resistance to these diseases is meditated by specific disease resistance genes-analogs (RGAs) which are differently distributed across B. oleracea lines. The sequenced reference cultivar does not contain all B. oleracea genes due to gene presence/absence variation between individuals, which makes it necessary to search for RGA candidates in the B...
September 19, 2018: Plant Biotechnology Journal
Ngoc-Ha Thi Tran, Taichi Oguchi, Nobuhumi Akatsuka, Etsuko Matsunaga, Akiyoshi Kawaoka, Akiyo Yamada, Yoshihiro Ozeki, Kazuo N Watanabe, Akira Kikuchi
The breeding of plantation forestry trees for the possible afforestation of marginal land would be one approach to addressing global warming issues. Here, we developed novel transgenic Eucalyptus trees (Eucalyptus camaldulensis Dehnh.) harboring an RNA-Binding-Protein (McRBP) gene derived from a halophyte plant, common ice plant (Mesembryanthemum crystallinum L.). We conducted screened-house trials of the transgenic Eucalyptus using two different stringency salinity stress conditions to evaluate the plants' acute and chronic salt stress tolerances...
September 19, 2018: Plant Biotechnology Journal
Xiongfeng Ma, Zhenyu Wang, Wei Li, Yuzhou Zhang, Xiaojian Zhou, Yangai Liu, Zhongying Ren, Xiaoyu Pei, Kehai Zhou, Wensheng Zhang, Kunlun He, Fei Zhang, Junfang Liu, Wenyu Ma, Guanghui Xiao, Daigang Yang
Upland cotton (Gossypium hirsutum) is the world's largest source of natural fiber and dominates the global textile industry. Hybrid cotton varieties exhibit strong heterosis that confers high fiber yields, yet the genome-wide effects of artificial selection that have influenced Upland cotton during its breeding history are poorly understood. Here, we re-sequenced Upland cotton genomes and constructed a variation map of an intact breeding pedigree comprising seven elite and 19 backbone parents. Compared to wild accessions, the 26 pedigree accessions underwent strong artificial selection during domestication that has resulted in reduced genetic diversity but stronger linkage disequilibrium and higher extents of selective sweeps...
September 16, 2018: Plant Biotechnology Journal
Nicholas D Bonawitz, W Michael Ainley, Asuka Itaya, Sivarama R Chennareddy, Tobias Cicak, Katherine Effinger, Ke Jiang, Tejinder Kumar Mall, Pradeep Reddy Marri, J Pon Samuel, Nagesh Sardesai, Matthew Simpson, Otto Folkerts, Rodrigo Sarria, Steven R Webb, Delkin O Gonzalez, Daina H Simmonds, Dayakar R Pareddy
Emerging genome editing technologies hold great promise for the improvement of agricultural crops. Several related genome editing methods currently in development utilize engineered, sequence-specific endonucleases to generate DNA double strand breaks (DSBs) at user-specified genomic loci. These DSBs subsequently result in small insertions/deletions (indels), base substitutions, or incorporation of exogenous donor sequences at the target site, depending on the application. Targeted mutagenesis in soybean (Glycine max) via non-homologous end joining (NHEJ)-mediated repair of such DSBs has been previously demonstrated with multiple nucleases, as has homology-directed repair (HDR)-mediated integration of a single transgene into target endogenous soybean loci using CRISPR/Cas9...
September 16, 2018: Plant Biotechnology Journal
Ning Zhang, Leah K McHale, John J Finer
Cis-regulatory elements in promoters are major determinants of binding specificity of transcription factors (TFs) for transcriptional regulation. To improve our understanding of how these short DNA sequences regulate gene expression, synthetic promoters consisting of both classical (CACGTG) and variant G-box core sequences along with different flanking sequences derived from the promoters of three different highly expressing soybean genes, were constructed and used to regulate a green fluorescent protein (gfp) gene...
September 6, 2018: Plant Biotechnology Journal
Na Li, Dongji Song, Wei Peng, Jiepeng Zhan, Jiaqin Shi, Xinfa Wang, Guihua Liu, Hanzhong Wang
Seed size/weight is one of the key traits related to plant domestication and crop improvement. In rapeseed (Brassica napus L.) germplasm, seed weight shows extensive variation, but its regulatory mechanism is poorly understood. To identify the key mechanism of seed weight regulation, a systematic comparative study was performed. Genetic, morphological and cytological evidence showed that seed weight was controlled by maternal genotype, primarily via the regulation of cell number. The physiological evidence indicated that differences in the pod length might result in differences in pod wall photosynthetic area, carbohydrates and the final seed weight...
September 6, 2018: Plant Biotechnology Journal
Ya-Fan Zhao, Ting Peng, Hong-Zheng Sun, Sachin Teotia, Hui-Li Wen, Yan-Xiu Du, Jing Zhang, Jun-Zhou Li, Gui-Liang Tang, Hong-Wei Xue, Quan-Zhi Zhao
Rice grain filling rate contributes largely to grain productivity and accumulation of nutrients. MicroRNAs (miRNAs) are key regulators of development and physiology in plants and become a novel key target for engineering grain size and crop yield. However, there is little studies, so far, showing the miRNA regulation of grain filling and rice yield, in consequence. Here, we show that suppressed expression of rice miR1432 (STTM1432) significantly improves grain weight by enhancing grain filling rate and leads to an increase in overall grain yield up to 17...
September 5, 2018: Plant Biotechnology Journal
Andrés Ortigosa, Selena Gimenez-Ibanez, Nathalie Leonhardt, Roberto Solano
Due to their different lifestyles, effective defence against biotrophic pathogens normally leads to increased susceptibility to necrotrophs, and vice versa. Solving this trade-off is a major challenge for obtaining broad-spectrum resistance in crops and requires uncoupling the antagonism between the jasmonate (JA) and salicylate (SA) defence pathways. Pseudomonas syringae pv. tomato (Pto) DC3000, the causal agent of tomato bacterial speck disease, produces coronatine (COR) that stimulates stomata opening and facilitates bacterial leaf colonization...
September 5, 2018: Plant Biotechnology Journal
Huixue Dong, Suli Yan, Jie Liu, Pan Liu, Jiaqiang Sun
Plant height is among the most important agronomic traits that influence crop yield. However, in addition to the Rht-1 alleles, the molecular basis of plant height in bread wheat remains largely unclear. Based on wheat gene expression profiling analysis, we identify a light-regulated gene from bread wheat, designated as TaCOLD1, whose encoding protein is homologous to cold sensor COLD1 in rice. We show that TaCOLD1 protein is localized to the endoplasmic reticulum (ER) and plasma membrane. Phenotypic analyses show that overexpression of a mutated form of TaCOLD1 (M187K) in bread wheat cultivar Kenong199 (Rht-B1b) background resulted in an obvious reduction in plant height...
September 5, 2018: Plant Biotechnology Journal
Baiquan Ma, Liao Liao, Ting Fang, Qian Peng, Collins Ogutu, Hui Zhou, Fengwang Ma, Yuepeng Han
Acidity is one of the main determinants of fruit organoleptic quality. Here, comparative transcriptome analysis was conducted between two cultivars that showed a significant difference in fruit acidity, but contained homozygous non-functional alleles at the major gene Ma1 locus controlling apple fruit acidity. A candidate gene for fruit acidity, designated M10, was identified. The M10 gene encodes a P-type proton pump, P3A -ATPase, which facilitates malate uptake into the vacuole. The Ma10 gene is significantly associated with fruit malate content, accounting for ~ 7...
September 5, 2018: Plant Biotechnology Journal
Jun Miao, Zefeng Yang, Dongping Zhang, Yuzhu Wang, Mengbin Xu, Lihui Zhou, Jun Wang, Shujun Wu, Youli Yao, Xi Du, Fangfei Gu, Zhiyun Gong, Minghong Gu, Guohua Liang, Yong Zhou
Heterotrimeric G proteins, which consist of Gα , Gβ and Gγ subunits, function as molecular switches to regulate a wide range of developmental processes in plants. In this study, we characterize the function of rice RGG2, which encodes a type B Gγ subunit, in grain size and yield production. The expression levels of RGG2 are significantly higher than those of other rice Gγ -encoding genes in all tissues tested, suggesting that RGG2 plays essential roles in rice growth and development. By regulating cell expansion, RGG2 overexpression in Nipponbare (NIP) leads to reduced plant height and decreased grain size...
August 30, 2018: Plant Biotechnology Journal
Iman Tabatabaei, Cristina Dal Bosco, Marta Bednarska, Stephanie Ruf, Jörg Meurer, Ralph Bock
The genetic transformation of plant cells is critically dependent on the availability of efficient selectable marker gene. Sulfonamides are herbicides that, by inhibiting the folic acid biosynthetic pathway, suppress the growth of untransformed cells. Sulfonamide resistance genes that were previously developed as selectable markers for plant transformation were based on the assumption that, in plants, the folic acid biosynthetic pathway resides in the chloroplast compartment. Consequently, the Sul resistance protein, a herbicide-insensitive dihydropteroate synthase, was targeted to the chloroplast...
August 25, 2018: Plant Biotechnology Journal
Xiaolan Rao, Xin Chen, Hui Shen, Qin Ma, Guifen Li, Yuhong Tang, Maria Pena, William York, Taylor P Frazier, Scott Lenaghan, Xirong Xiao, Fang Chen, Richard A Dixon
Cell wall recalcitrance is the major challenge to improving saccharification efficiency in converting lignocellulose into biofuels. However, information regarding the transcriptional regulation of secondary cell wall biogenesis remains poor in switchgrass (Panicum virgatum), which has been selected as a biofuel crop in the United States. In this study, we present a combination of computational and experimental approaches to develop gene regulatory networks for lignin formation in switchgrass. To screen transcription factors (TFs) involved in lignin biosynthesis, we developed a modified method to perform co-expression network analysis using 14 lignin biosynthesis genes as bait (target) genes...
August 22, 2018: Plant Biotechnology Journal
Paul Daly, Christopher McClellan, Marta Maluk, Helena Oakey, Catherine Lapierre, Robbie Waugh, Jennifer Stephens, David Marshall, Abdellah Barakate, Yukiko Tsuji, Geert Goeminne, Ruben Vanholme, Wout Boerjan, John Ralph, Claire Halpin
Caffeic acid O-methyltransferase (COMT), the lignin biosynthesis gene modified in many brown-midrib high-digestibility mutants of maize and sorghum, was targeted for downregulation in the small grain temperate cereal, barley (Hordeum vulgare), to improve straw properties. Phylogenetic and expression analyses identified the barley COMT orthologue(s) expressed in stems, defining a larger gene family than in brachypodium or rice with three COMT genes expressed in lignifying tissues. RNAi significantly reduced stem COMT protein and enzyme activity, and modestly reduced stem lignin content while dramatically changing lignin structure...
August 22, 2018: Plant Biotechnology Journal
Qi-Jun Ma, Mei-Hong Sun, Jing Lu, Hui Kang, Chun-Xiang You, Yu-Jin Hao
Sugars increase with drought stress in plants and accumulate in the vacuole. However, the exact molecular mechanism underlying this process is not clear yet. In this study, protein interaction and phosphorylation experiments were conducted for sucrose transporter and CIPK kinase in apple. The specific phosphorylation site of sucrose transporter was identified with mass spectrometry. Transgenic analyses were performed to characterize their biological function. It was found that overexpression of sucrose transporter gene MdSUT2...
August 22, 2018: Plant Biotechnology Journal
Qingzhang Du, Xiaohui Yang, Jianbo Xie, Mingyang Quan, Liang Xiao, Wenjie Lu, Jiaxing Tian, Chenrui Gong, Jinhui Chen, Bailian Li, Deqiang Zhang
In perennial woody plants, the coordinated increase of stem height and diameter during juvenile growth improves competitiveness (i.e. access to light); however, the factors underlying variation in stem growth remain unknown in trees. Here, we used linkage-linkage disequilibrium (linkage-LD) mapping to decipher the genetic architecture underlying three growth traits during juvenile stem growth. We used two Populus populations: a linkage mapping population comprising a full-sib family of 1200 progeny and an association mapping panel comprising 435 unrelated individuals from nearly the entire natural range of P...
August 22, 2018: Plant Biotechnology Journal
Sebastian Pfeilmeier, Jeoffrey George, Arry Morel, Sonali Roy, Matthew Smoker, Lena Stransfeld, J Allan Downie, Nemo Peeters, Jacob G Malone, Cyril Zipfel
Interfamily transfer of plant pattern recognition receptors (PRRs) represents a promising biotechnological approach to engineer broad-spectrum, and potentially durable, disease resistance in crops. It is however unclear whether new recognition specificities to given pathogen-associated molecular patterns (PAMPs) affect the interaction of the recipient plant with beneficial microbes. To test this in a direct reductionist approach, we transferred the Brassicaceae-specific PRR ELONGATION FACTOR-THERMO UNSTABLE RECEPTOR (EFR), conferring recognition of the bacterial EF-Tu protein, from Arabidopsis thaliana to the legume Medicago truncatula...
August 18, 2018: Plant Biotechnology Journal
Hong Chen, Hao Feng, Xueyan Zhang, Chaojun Zhang, Tao Wang, Jiangli Dong
The HUB2 gene encoding histone H2B monoubiquitination E3 ligase is involved in seed dormancy, flowering timing, defence response, and salt stress regulation in Arabidopsis thaliana. In this study, we used the cauliflower mosaic virus (CaMV) 35S promoter to drive AtHUB2 overexpression in cotton and found that it can significantly improve the agricultural traits of transgenic cotton plants under drought stress conditions, including increasing the fruit branch number, boll number, and boll-setting rate and decreasing the boll abscission rate...
August 17, 2018: Plant Biotechnology Journal
Miles R Armstrong, Jack Vossen, Tze Yin Lim, Ronald C B Hutten, Jianfei Xu, Shona M Strachan, Brian Harrower, Nicolas Champouret, Eleanor M Gilroy, Ingo Hein
Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost-effective in monitoring resistances than whole-genome sequencing and can be used to appraise (trans) gene integrity efficiently...
August 14, 2018: Plant Biotechnology Journal
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"