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Journal of Molecular Histology

Qiulan Li, Yue Guo, Mianfeng Yao, Jun Li, Yingyi Chen, Qiong Liu, Yun Chen, Yuanyuan Zeng, Bin Ji, Yunzhi Feng
Cdkn1c, a member of the Cip/Kip cyclin-dependent kinase inhibitor family, is critically involved in regulating cell cycle and cellular differentiation during development in mammals. However, the functional role of Cdkn1c and the underlying mechanisms by which Cdkn1c affects odontogenesis remain largely unknown. In our study, we found that Cdkn1c expression dynamically changes from embryonic day 11.5 (E11.5) to postnatal day 3 (P3), and exhibits tissue-specific expression profiles. Evaluation of CDKN1C protein by immunohistochemistry and western blot, revealed that CDKN1C protein expression peaks at P3 and then is reduced at P5 and P7...
July 16, 2018: Journal of Molecular Histology
Kun Yu, Yuanhang Ma, Zhicao Zhang, Xin Fan, Teming Li, Liangzi Li, Weidong Xiao, Yujiao Cai, Lihua Sun, Pengyuan Xu, Min Yu, Hua Yang
The Par complex (Par-6/Par-3/aPKC) plays a key role in the maintenance of the intestinal barrier function through the regulation of epithelial junction formation. The aryl hydrocarbon receptor (AhR) has been shown to be an important regulator for intestinal homeostasis. In this study, we investigated the role of the AhR activation on the regulation of Par complex. AhR activation by 6-formylindolo (3,2-b) carbazole (FICZ) represses the abnormal expression of the Par complex in a mouse model of dextran sulphate sodium (DSS)-induced colitis...
July 10, 2018: Journal of Molecular Histology
Xinhua Wang, Yuan Yu
Myocardial dysfunction is a major cause of death in sepsis. MicroRNA-146b (miR-146b) has been reported to be related to myocardial disease. However, the role of miR-146b in sepsis as well as myocardial injury is still unclear. Septic cardiac dysfunction in mice was induced by cecal ligation and puncture (CLP) and miR-146b was found increased significantly in the myocardium tissue of CLP mice. It was found that up-regulation of miR-146b by agomiR injection suppressed expression of IL-1β in mice as well as myocardium apoptosis in CLP mice...
June 27, 2018: Journal of Molecular Histology
Lachlan Harris, Oressia Zalucki, Michael Piper
In the original publication of the article, two errors were made in describing the equations for Tc .
June 27, 2018: Journal of Molecular Histology
Michelangelo Nigro, Claudia De Sanctis, Pietro Formisano, Rosita Stanzione, Maurizio Forte, Giovambattista Capasso, Giuseppe Gigliotti, Speranza Rubattu, Davide Viggiano
The uncoupling protein-2 (UCP2) is an anion transporter that plays a key role in the control of intracellular oxidative stress. In animal models UCP2 downregulation has several pathological sequelae, particularly affecting the vasculature and the kidney. Specifically, in these models kidney damage is highly favored in the absence of UCP2 in the context of experimental hypertension. Confirmations of these data in humans awaits further information, as no data are yet available concerning the cell-type and subcellular expression in the human kidney...
June 23, 2018: Journal of Molecular Histology
Xue Liu, Yingying Liu, Zaofeng Yang, Wen Ning
Follistatin like-1 (Fstl1) is a secreted glycoprotein and can be up-regulated by TGF-β1. To better study the function of Fstl1 in lung development, we examined Fstl1 expression in the developing lung, in a cell type specific manner, using a tamoxifen inducible Fstl1-reporter mouse strain. Our results show that Fstl1 is ubiquitously expressed at saccular stage in the developing lung. At E18.5, Fstl1 expression is robust in most type of mesenchymal cells, including airway smooth muscle cells surrounding airways, vascular smooth muscle cells, endothelial cells, and vascular pericytes from blood vessel, but not PDGFRα+ fibroblasts in the distal alveolar sacs...
June 18, 2018: Journal of Molecular Histology
Xijiao Yu, Shuang Liu, Hui Chen, Xinyu Zhao, Xue Chen, Yi Du, Shu Li
Calcitonin gene-related peptide (CGRP) is a marked and important neuropeptide expressed in nerve fibers during bone repair. This study investigated the role of CGRP overexpression on osteogenic differentiation of rat bone mesenchymal stem cells (rBMSCs). rBMSCs were infected with viral stocks of pLenO-DCE-CGRP (CGRP group) or pLenO-DCE (Vector group), while normal rBMSCs were used as a control. Transfection efficiency of rBMSCs was analyzed by flow cytometry. Cell proliferation was examined using a Cell Counting Kit-8 and flow cytometry...
May 30, 2018: Journal of Molecular Histology
Cong-Cong Wu, Hao Li, Yao Xiao, Lei-Lei Yang, Lei Chen, Wei-Wei Deng, Lei Wu, Wen-Feng Zhang, Zhi-Jun Sun
IQ-domain GTPase-activating protein 1 (IQGAP1) is associated with the development and progression of many human cancers. We aimed to investigate the expression and clinicopathological significances of IQGAP1 in head and neck squamous cell carcinoma (HNSCC). In this study, immunohistochemical staining of IQGAP1, co-inhibitory immune checkpoint molecules and macrophage markers were performed in human HNSCC samples to analyze the expression and correlation with clinicopathological characteristics. Immunoreactivity of IQGAP1 was also detected in immunocompetent mouse HNSCC tissue...
May 30, 2018: Journal of Molecular Histology
Xue Wu, Jun Li, Xuekang Yang, Xiaozhi Bai, Jihong Shi, Jianxin Gao, Yan Li, Shichao Han, Yijie Zhang, Fu Han, Yang Liu, Xiaoqiang Li, Kejia Wang, Julei Zhang, Zheng Wang, Ke Tao, Dahai Hu
Hypertrophic scar (HS) is a serious skin fibrotic disease characterized by the excessive proliferation of fibroblasts and often considered as a kind of benign skin tumor. microRNA-155 (miR-155) is usually served as a promising marker in antitumor therapy. In view of the similarities of hypertrophic scar and tumor, it is predicted that miR-155 may be a novel therapeutic target in clinical trials. Here we found the expression levels of miR-155 was gradually down regulated and HIF-1α was upregulated in HS tissue and HS derived fibroblasts (HFs)...
May 21, 2018: Journal of Molecular Histology
Qiao Qiao, Xiaoru Xu, Yingliang Song, Shuang Song, Wenzhong Zhu, Fenglan Li
Bone regeneration is impaired in patients with type 2 diabetes mellitus (T2DM), which leads to non-healing after bone loss. The decreased osteogenic capacity of bone mesenchymal stem cells (BMSCs) might be a main reason. Sema3A, as a powerful protein promoting osteocyte differentiation, shows potential for bone regeneration treatment. BMSCs may be a therapeutic solution. In this study, we divided BMSCs from T2DM rats (BMSCs-D) and normal rats (BMSCs-N), identified their ability to differentiate into different cell types...
May 17, 2018: Journal of Molecular Histology
Roberto Tamma, Tiziana Annese, Simona Ruggieri, Andrea Marzullo, Beatrice Nico, Domenico Ribatti
Gastric cancer is the fifth most common cancer and third leading cause of cancer-related death worldwide. Several studies on angiogenic blocking agents in gastric cancer revealing promising results by the use of monoclonal antibodies against VEGFA or its receptor VEGFR2 or against VEGFA activating pathway. The validation of biomarkers useful to better organize the clinical trials involving anti-angiogenic therapies is crucial. Molecular markers such as RNA are increasingly used for cancer diagnosis, prognosis, and therapy guidance as in the case of the targeted therapies concerning the inhibition of angiogenesis...
May 14, 2018: Journal of Molecular Histology
Lawrence Owusu, Caiming Xu, Hailong Chen, Geliang Liu, Guixin Zhang, Jinwen Zhang, Zhankai Tang, Zhongwei Sun, Xin Yi
Severe acute pancreatitis (SAP) associated acute lung injury (ALI) accounts for about 70% mortality of SAP patients. However, there are no precise biomarkers for the disease currently. Herein, we evaluated the potential of gamma-enolase (ENO2), against its universal isoform alpha-enolase (ENO1), as a marker of SAP-ALI in a rat model. Firstly, 16 male Sprague-Dawley rats were randomly divided into two groups, Sham (n = 8) and SAP-ALI (n = 8), for pancreatitis induction. Ultra-structure examination by electron microscopy and HE staining were used for lung injury assessment...
May 4, 2018: Journal of Molecular Histology
Yunfei Wang, Mengmeng Liu, Shijian Deng, Xin Sui, Linlin Fan, Qi Zhang
Epithelial rests of Malassez (ERM), the only odontogenic epithelial structures in periodontal tissue, are proposed to correlate with root resorption, but the detailed mechanism remains unclear. Osteoprotegerin (OPG), the main inhibitor of osteoclastogenesis, plays a pivotal role in inhibiting root resorption, and ERM cells express OPG mRNA in vitro. Thus, in this study, we aimed to clarify OPG expression in ERM in vivo and to explore the role of OPG in ERM to determine whether ERM are associated with root resorption via OPG...
June 2018: Journal of Molecular Histology
Fang Wang, Bei Liu, Zhou Yu, Tong Wang, Yajuan Song, Ran Zhuang, Yonghong Wu, Yingjun Su, Shuzhong Guo
Diabetes is a condition that causes delayed wound healing and results in chronic wounds. CD100 has been reported to promote and induce potent and obvious angiogenesis both in vivo and in vitro studies, the absence of which are a main cause of the diabetic chronic wound. In the present study, we investigated the effects of application of soluble CD100 on wound healing in diabetic mice. Four 5-mm full-thickness dermal wounds were made on each male db/db mouse. 12 mice from CD100 group were subcutaneously injected with 250 ng of CD100 (50 µl) per wound, in addition, 12 mice were injected with the same volume phosphate-balanced solution as the control...
June 2018: Journal of Molecular Histology
Jia Shi, Zhengtao Lv, Mingbo Nie, Weiwei Lu, Changyu Liu, Yong Tian, Long Li, Guoxiang Zhang, Ranyue Ren, Ziyang Zhang, Hao Kang
The nail is a continuous skin appendage. Cells located around the nails, which display coordinated homeostatic dynamics and release a flow of stem cells in response to regeneration, have been identified in mice. However, very few studies regarding human nail stem cells exist in the literature. Using specimens isolated from humans, we detected an unreported population of cells within the basal layer of postnatal human nail proximal folds (NPFs) and the nail matrix around the nail root. These cells were multi-expressing and expressed stem cell markers, such as keratin 15 (K15), keratin 14 (K14), keratin 19 (K19), CD29, CD34, and leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6)...
June 2018: Journal of Molecular Histology
Xiaofang Wang, Zhan Zhang, Xianxu Zeng, Jinming Wang, Linlin Zhang, Wanyu Song, Ying Shi
This study aims to elucidate the mechanisms of Wnt/β-catenin signaling pathway in the development of preeclampsia (PE). The mRNA levels of Wnt1, β-catenin, c-myc and cyclinD1 were determined by real-time PCR in the placentas. Moreover, the expression levels of Wnt1, β-catenin, Dickkopf-1 (DKK1) and glycogen synthase kinase 3β (GSK-3β) proteins were detected by Western blot. Immunohistochemistry was used in placental tissue microarray to localize the expression of Wnt1, β-catenin, DKK1 proteins in the placentas of two groups...
June 2018: Journal of Molecular Histology
Radwa Ayman Salah, Ihab K Mohamed, Nagwa El-Badri
Developing effective stem cell-based therapies requires the design of complex in vitro culture systems for accurate representation of the physiological stem cell niche. Human amniotic membrane (hAM) has been successfully used in clinical grafting applications due to its unique biological and regenerative properties. Decellularized hAM (d-hAM) has been previously applied to the culture of human bone marrow mesenchymal stem cells (hMSCs), promoting their expansion and differentiation into adipogenic and osteogenic lineages...
June 2018: Journal of Molecular Histology
Sang-Joun Yu, Yoon Seon Lee, Han-Wool Choung, Yeoung-Hyun Park, Byung-Ock Kim, Joo-Cheol Park
Preameloblast-conditioned medium (PACM) has been reported as a potent dentin regenerative material, but its effects as a mixture on periodontal regeneration and the role of CPNE7 in PACM are not known. The purpose of this study is to evaluate the histologic and histomorphometric effects of preameloblast-conditioned medium (PACM) and CPNE7 on periodontal tissue healing in dogs. Seventy-two mandibular premolar roots from ten dogs were extracted and randomly divided into six groups (n = 12 each): (1) positive control group; (2) negative control group; (3) cementum-removed and PACM-treated group; (4) cementum-preserved and PACM-treated group; (5) CPNE7-inactivated PACM-treated group; and (6) recombinant CPNE7-treated group...
June 2018: Journal of Molecular Histology
Haichao Liu, Lei Zhao, Wenzhen Gu, Qin Liu, Zhixiong Gao, Xiao Zhu, Zhi Wu, Hongwen He, Fang Huang, Wenguo Fan
Satellite glial cells (SGCs), a peripheral neuroglial cell, surround neurons and form a complete envelope around individual sensory neurons in the trigeminal ganglia (TG), which may be involved in modulating neurons in inflammation. The purpose of this study was to determine the effect of dental injury and inflammation on SGCs in the TG. Pulp exposure (PX) was performed on the first maxillary molar of 28 rats. The neurons innervating injured tooth in TG were labeled by the retrograde transport of fluoro-gold (FG)...
June 2018: Journal of Molecular Histology
M Ioannou, E Kouvaras, R Papamichali, M Samara, I Chiotoglou, G Koukoulis
Epithelial-mesenchymal transition (EMT) plays an important role in cancer metastasis. During EMT, tumor cells acquire the capacity to migrate and invade the stroma. Activation of the transforming growth factor-b (TGF-b) signaling pathway is of major importance for the initiation of EMT. Smad4, an essential protein of this pathway, is known to complex with multiple transcription factors (e.g. Snail-1, Slug, Twist-1), in various types of cancer, promoting the repression or activation of target genes. The role of Smad4 in colorectal cancer (CRC) is not straightforward so far...
June 2018: Journal of Molecular Histology
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