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Journal of Molecular Histology

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https://www.readbyqxmd.com/read/27913976/follistatin-like-protein-1-suppressed-pro-inflammatory-cytokines-expression-during-neuroinflammation-induced-by-lipopolysaccharide
#1
Kai-Yuan Cheng, Yi Liu, Ying-Guang Han, Jing-Kun Li, Jia-Lin Jia, Bin Chen, Zhi-Xiao Yao, Lin Nie, Lei Cheng
Follistain-like protein 1 (FSTL1), has been recently demonstrated to be involved in the embryo development of nervous system and glioblastoma. However, the role of FSTL1 in neuroinflammation remains unexplored. In this study, the expression of FSTL1 in astrocytes was verified and its role was studied in neuroinflammation induced by in vivo intracerebroventricular (ICV) injection of lipopolysaccharide (LPS) or LPS treatment to astrocytes in vitro. FSTL1 was significantly induced after ICV LPS injection or LPS treatment...
December 2, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27885497/erratum-to-a-zebrafish-mosaic-assay-to-study-mammalian-stem-cells-in-real-time-in-vivo
#2
Chun Xiao, Meilin Qian, Chaoran Yin, Yonggang Zhang, Huozhen Hu, Shaohua Yao
No abstract text is available yet for this article.
November 24, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27882438/histochemical-examination-of-systemic-administration-of-eldecalcitol-combined-with-guided-bone-regeneration-for-bone-defect-restoration-in-rats
#3
Xiuchun Han, Juan Du, Di Liu, Hongrui Liu, Norio Amizuka, Minqi Li
The aim of this experiment was to elucidate the histological alterations after systemic administration of eldecalcitol (ELD) combined with guided bone regeneration during the restoration of bone defect healing in rats. The femurs of 8-week-old Wister rats were used to generate bone defect models. The defect was covered with a collagen membrane, and ELD group was administrated with eldecalcitol (50 ng/kg body weight) intragastrically once every other day. Femora were harvested at 1, 2, 4 and 8 weeks post-surgery...
November 23, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27866302/expression-analysis-of-dickkopf-related-protein-3-dkk3-suggests-its-pleiotropic-roles-for-a-secretory-glycoprotein-in-adult-mouse
#4
Junji Inoue, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Hiromi Kumon, Hideyo Ohuchi
Dickkopf-related protein 3 (Dkk3) is the third member of the Dkk gene family and identical to the gene, whose expression was reduced in immortalized cells. Therefore, its another name is reduced expression in immortalized cells. Since the intratumoral introduction of Dkk3 inhibits tumor growth in mouse models of cancers, Dkk3 is likely a tumor suppressor gene. However, the functions of Dkk3 in vivo remain unclear. As the first step to decipher the physiological roles of this gene, we examined the expression pattern of Dkk3 in various tissues from adult mice...
November 19, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27803997/evaluating-the-effect-of-silver-nanoparticles-on-testes-of-adult-albino-rats-histological-immunohistochemical-and-biochemical-study
#5
Samah M Ahmed, Shaimaa A Abdelrahman, Sally M Shalaby
: Silver nanoparticles (AgNPs) are widely used in medicine, however, they have toxic impacts on different organs. AgNPs distribution to the testes was reported, so, we aimed to study the effect of intraperitoneal injection of AgNPs, at different concentrations and different time durations, on adult rat testes. Sixty healthy adult male Wistar albino rats were divided into three groups; control group (Group I) and two experimental groups (Groups II & III), each of which were subdivided into two subgroups...
November 1, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27803996/crim1-is-necessary-for-coronary-vascular-endothelial-cell-development-and-homeostasis
#6
Swati Iyer, Yash Chhabra, Tracey J Harvey, Richard Wang, Han Sheng Chiu, A G Smith, Walter G Thomas, David J Pennisi, Michael Piper
Endothelial cells form a critical component of the coronary vasculature, yet the factors regulating their development remain poorly defined. Here we reveal a novel role for the transmembrane protein CRIM1 in mediating cardiac endothelial cell development. In the absence of Crim1 in vivo, the coronary vasculature is malformed, the number of endothelial cells reduced, and the canonical BMP pathway dysregulated. Moreover, we reveal that CRIM1 can bind IGFs, and regulate IGF signalling within endothelial cells...
November 1, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27787633/foxa1-gene-and-protein-in-developing-rat-eccrine-sweat-glands
#7
Haihong Li, Liyun Chen, Mingjun Zhang, Bingna Zhang
To investigate the development of eccrine sweat glands and the expression of Foxa1 genes and proteins in the course of development, the footpads from E15.5 to E21.5, P1-P12, P14, P21, P28 and P56 rats were subjected to immunofluorescence staining of FoxA1 and double immunofluorescence staining of K14/α-SMA, FoxA1/K7 and FoxA1/α-SMA, and were processed for Foxa1 gene detection by RT-qPCR. The results showed that rat eccrine sweat gland germs was first observed emerging from the basal layer of epidermis at E19...
October 27, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27722791/a-modified-protocol-for-the-detection-of-three-different-mrnas-with-a-new-generation-in-situ-hybridization-chain-reaction-on-frozen-sections
#8
Qian-Qian Sui, Jiao Zhu, Xiangnan Li, Gillian E Knight, Cheng He, Geoffrey Burnstock, Hongbin Yuan, Zhenghua Xiang
A new multiple fluorescence in situ hybridization method based on hybridization chain reaction was recently reported, enabling simultaneous mapping of multiple target mRNAs within intact zebrafish and mouse embryos. With this approach, DNA probes complementary to target mRNAs trigger chain reactions in which metastable fluorophore-labeled DNA hairpins self-assemble into fluorescent amplification polymers. The formation of the specific polymers enhances greatly the sensitivity of multiple fluorescence in situ hybridization...
October 8, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27714564/heat-shock-protein-27-hsp27-regulates-stat3-and-eif4g-levels-in-first-trimester-human-placenta
#9
Gali Epstein Shochet, Oded Komemi, Dana Sadeh-Mestechkin, Meir Pomeranz, Ami Fishman, Liat Drucker, Michael Lishner, Shelly Tartakover Matalon
During placental implantation, cytotrophoblast cells differentiate to extravillous trophoblast (EVT) cells that invade from the placenta into the maternal uterine blood vessels. The heat shock protein-27 (HSP27), the signal transducer and activator of transcription-3 (STAT3) and the eukaryotic translation initiation factor 4E (EIF4E) are involved in regulating EVT cell differentiation/migration. EIF4E and EIF4G compose the translation initiation complex, which is a major control point in protein translation...
October 6, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27696080/determining-protein-biomarkers-for-dlbcl-using-ffpe-tissues-from-hiv-negative-and-hiv-positive-patients
#10
Pumza Magangane, Raveendra Sookhayi, Dhirendra Govender, Richard Naidoo
DLBCL is the most common lymphoma subtype occurring in older populations as well as in younger HIV infected patients. The current treatment options for DLBCL are effective for most patients yet the relapse rate is high. While many biomarkers for DLBCL exist, they are not in clinical use due to low sensitivity and specificity. In addition, these biomarkers have not been studied in the HIV context. Therefore, the identification of new biomarkers for HIV negative and HIV positive DLBCL, may lead to a better understanding of the disease pathology and better therapeutic design...
October 1, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27650519/n-cadherin-regulates-beta-catenin-signal-and-its-misexpression-perturbs-commissural-axon-projection-in-the-developing-chicken-spinal-cord
#11
Ciqing Yang, Xiaoying Li, Congrui Wang, Sulei Fu, Han Li, Zhikun Guo, Shanting Zhao, Juntang Lin
N-cadherin is a calcium-sensitive cell adhesion molecule that plays an important role in the formation of the neural circuit and the development of the nervous system. In the present study, we investigated the function of N-cadherin in cell-cell connection in vitro with HEK293T cells, and in commissural axon projections in the developing chicken spinal cord using in ovo electroporation. Cell-cell connections increased with N-cadherin overexpression in HEK293T cells, while cell contacts disappeared after co-transfection with an N-cadherin-shRNA plasmid...
September 20, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27681986/age-dependent-impact-of-inferior-alveolar-nerve-transection-on-mandibular-bone-metabolism-and-the-underlying-mechanisms
#12
Qingqing Wu, Bo Yang, Cong Cao, Mengkai Guang, Ping Gong
Aging is associated with peripheral nerve degradation and bone destruction. The aim of the study is to elucidate the influence of sensory denervation on bone metabolism in different age groups by establishing a modified unilateral inferior alveolar nerve transection (IANT) model. The rats, divided into young, middle-aged and aged group, were sacrificed at 1, 2, 4 and 8 weeks after right IANT. The histological changes of mandibles were analyzed by fluorescent double labeling, micro-CT, HE, TRAP and anti-CGRP immunohistochemical staining...
December 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27558143/hyperlipidemia-induced-by-high-fat-diet-enhances-dentin-formation-and-delays-dentin-mineralization-in-mouse-incisor
#13
Xin Ye, Jin Zhang, Pishan Yang
Dyslipidemia has become a serious health problem in children and adolescents worldwide for its high prevalence. Since hard tissues of permanent teeth form mainly during this period and lipids are actively involved in tooth development, the effects of hyperlipidemia on dental tissue formation and mineralization need to be illustrated. In this study, hyperlipidemia model was established in mice fed with high-fat diet (HFD). Micro-CT and histomorphological analyses were performed on the mandibular bones to assess the morphological changes of the mandibular incisor and first molar...
October 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27554369/a-zebrafish-mosaic-assay-to-study-mammalian-stem-cells-in-real-time-in-vivo
#14
REVIEW
Chun Xiao, Meilin Qian, Chaoran Yin, Yonggang Zhang, Huozhen Hu, Shaohua Yao
The differentiation potentials of stem cells have been evaluated by various in vivo and in vitro assays. However, these assays have different limitations hindering efficient study of mammalian stem cells. Here we describe a rapid and powerful mosaic assay to study the differentiation potentials of stem cells in real time in vivo by using zebrafish embryo. We transplanted mouse neural stem cells into zebrafish embryos at different developmental stages and found that they mainly formed neural tissues while occasionally trans-differentiated into mesoderm- and endoderm-derived tissues...
October 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27541036/ovarian-follicular-dynamics-after-aromatizable-or-non-aromatizable-neonatal-androgenization
#15
Gabriel Anesetti, Rebeca Chávez-Genaro
The effects of neonatal testosterone or dihydrotestosterone exposure on ovarian follicular dynamics were analysed at prepubertal, pubertal or adult age in Wistar rats. Both androgens induced a transitory increase on follicular endowment that was partially corrected at puberty. At adult age testosterone prevented ovulation, without significant modifications on follicular dynamics. An increased number of cystic structures were observed from puberty to adult age. However, ovaries of rats treated with dihydrotestosterone showed follicles with evident morphological alterations in granulosa and thecal layers although several corpora lutea were observed...
October 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27541035/osteoprotegerin-deficiency-leads-to-deformation-of-the-articular-cartilage-in-femoral-head
#16
Yi Liu, Jianping Ge, Danying Chen, Yuteng Weng, Haiming Du, Yao Sun, Qi Zhang
Osteoarthritis (OA) was a degenerative joint disease characterized by articular cartilage degradation and extensive remodeling of the subchondral bone. Multiple lines of evidence indicated that Osteoprotegerin (OPG), a member of TNF receptor superfamily that was expressed in the chondrocytes of articular cartilage and adjacent locations in the physiological setting, was involved in maintaining integrity of articular cartilage. OPG could prevent subchondral bone from resorption, and also protect cartilage from degradation...
October 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27541034/syndecan-4-regulates-the-bfgf-induced-chemotactic-migration-of-endothelial-cells
#17
Ran Li, Han Wu, Jun Xie, Guannan Li, Rong Gu, Lina Kang, Lian Wang, Biao Xu
Chemotactic migration of endothelial cells (ECs) guided by extracellular attractants is essential for blood vessel formation. Synd4 is a ubiquitous heparin sulfate proteoglycan receptor on the cell surface that has been identified to promote angiogenesis during tissue repair. Here, the role synd4 played in chemotactic migration of ECs was investigated in vitro and in vivo. Human umbilical vein endothelial cells (HUVECs) were transfected with Lenti-synd4-RNAi or Lenti-null. Cell migration was observed in a 2D-chemotaxis slide with a stable gradient of basic fibroblast growth factor (bFGF) for 18 h using time-lapse microscopy...
October 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27492422/human-eccrine-sweat-gland-cells-reconstitute-polarized-spheroids-when-subcutaneously-implanted-with-matrigel-in-nude-mice
#18
Haihong Li, Mingjun Zhang, Liyun Chen, Xuexue Li, Bingna Zhang
Increasing evidence indicates that maintenance of cell polarity plays a pivotal role in the regulation of glandular homeostasis and function. We examine the markers for polarity at different time points to investigate the formation of cell polarity during 3D reconstitution of eccrine sweat glands. Mixtures of eccrine sweat gland cells and Matrigel were injected subcutaneously into the inguinal regions of nude mice. At 2, 3, 4, 5 and 6 weeks post-implantation, Matrigel plugs were removed and immunostained for basal collagen IV, lateral β-catenin, lateroapical ZO-1 and apical F-actin...
October 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27456852/wnt5a-mediated-canonical-wnt-signaling-pathway-activation-in-orthodontic-tooth-movement-possible-role-in-the-tension-force-induced-bone-formation
#19
Hai-Di Fu, Bei-Ke Wang, Zi-Qiu Wan, Heng Lin, Mao-Lin Chang, Guang-Li Han
Orthodontic tooth movement (OTM) is associated with bone remodeling mediated by orthodontic mechanical loading. Increasing studies reported that Wnt signaling played crucial roles in mechanical stimuli induced bone remodeling. However, little is known about the involvement of Wnt signaling in orthodontic force-induced bone formation during OTM. In virtue of the OTM mice model as we previously reported, where new bone formation was determined by micro-CT and immunoreactivity of osteocalcin and osterix, we explored the activation of Wnt signaling pathway during OTM...
October 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27394426/maturation-of-cortical-bone-suppresses-periosteal-osteoprogenitor-proliferation-in-a-paracrine-manner
#20
Young Jae Moon, Chi-Young Yun, Jeong-Chae Lee, Jung Ryul Kim, Byung-Hyun Park, Eui-Sic Cho
Periosteum contains enriched pools of osteogenic progenitors and is highly proliferative, thus giving this tissue a pivotal role in maintaining the diameter of the diaphyseal cortex and in recovery from fractures. Although periosteal proliferation has not been detected in normal bone, intense periosteal proliferation has been observed in pathologic states such as fracture, inflammation, and bone tumors. However, the mechanism by which periosteal osteoprogenitor proliferation is regulated remains poorly understood...
October 2016: Journal of Molecular Histology
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