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Journal of Molecular Histology

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https://www.readbyqxmd.com/read/29785488/mir-155-inhibits-the-formation-of-hypertrophic-scar-fibroblasts-by-targeting-hif-1%C3%AE-via-pi3k-akt-pathway
#1
Xue Wu, Jun Li, Xuekang Yang, Xiaozhi Bai, Jihong Shi, Jianxin Gao, Yan Li, Shichao Han, Yijie Zhang, Fu Han, Yang Liu, Xiaoqiang Li, Kejia Wang, Julei Zhang, Zheng Wang, Ke Tao, Dahai Hu
Hypertrophic scar (HS) is a serious skin fibrotic disease characterized by the excessive proliferation of fibroblasts and often considered as a kind of benign skin tumor. microRNA-155 (miR-155) is usually served as a promising marker in antitumor therapy. In view of the similarities of hypertrophic scar and tumor, it is predicted that miR-155 may be a novel therapeutic target in clinical trials. Here we found the expression levels of miR-155 was gradually down regulated and HIF-1α was upregulated in HS tissue and HS derived fibroblasts (HFs)...
May 21, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29774455/semaphorin-3a-promotes-osteogenic-differentiation-of-bmsc-from-type-2-diabetes-mellitus-rats
#2
Qiao Qiao, Xiaoru Xu, Yingliang Song, Shuang Song, Wenzhong Zhu, Fenglan Li
Bone regeneration is impaired in patients with type 2 diabetes mellitus (T2DM), which leads to non-healing after bone loss. The decreased osteogenic capacity of bone mesenchymal stem cells (BMSCs) might be a main reason. Sema3A, as a powerful protein promoting osteocyte differentiation, shows potential for bone regeneration treatment. BMSCs may be a therapeutic solution. In this study, we divided BMSCs from T2DM rats (BMSCs-D) and normal rats (BMSCs-N), identified their ability to differentiate into different cell types...
May 17, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29761299/vegfa-and-vegfr2-rnascope-determination-in-gastric-cancer
#3
Roberto Tamma, Tiziana Annese, Simona Ruggieri, Andrea Marzullo, Beatrice Nico, Domenico Ribatti
Gastric cancer is the fifth most common cancer and third leading cause of cancer-related death worldwide. Several studies on angiogenic blocking agents in gastric cancer revealing promising results by the use of monoclonal antibodies against VEGFA or its receptor VEGFR2 or against VEGFA activating pathway. The validation of biomarkers useful to better organize the clinical trials involving anti-angiogenic therapies is crucial. Molecular markers such as RNA are increasingly used for cancer diagnosis, prognosis, and therapy guidance as in the case of the targeted therapies concerning the inhibition of angiogenesis...
May 14, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29728894/gamma-enolase-predicts-lung-damage-in-severe-acute-pancreatitis-induced-acute-lung-injury
#4
Lawrence Owusu, Caiming Xu, Hailong Chen, Geliang Liu, Guixin Zhang, Jinwen Zhang, Zhankai Tang, Zhongwei Sun, Xin Yi
Severe acute pancreatitis (SAP) associated acute lung injury (ALI) accounts for about 70% mortality of SAP patients. However, there are no precise biomarkers for the disease currently. Herein, we evaluated the potential of gamma-enolase (ENO2), against its universal isoform alpha-enolase (ENO1), as a marker of SAP-ALI in a rat model. Firstly, 16 male Sprague-Dawley rats were randomly divided into two groups, Sham (n = 8) and SAP-ALI (n = 8), for pancreatitis induction. Ultra-structure examination by electron microscopy and HE staining were used for lung injury assessment...
May 4, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29675567/human-urine-derived-stem-cells-play-a-novel-role-in-the-treatment-of-stz-induced-diabetic-mice
#5
Tianxue Zhao, Deng Luo, Yun Sun, Xin Niu, Yang Wang, Chen Wang, Weiping Jia
Human urine-derived stem cells (hUSCs) are a potential stem cell source for cell therapy. However, the effect of hUSCs on glucose metabolism regulation in type 1 diabetes was not clear. Therefore, the aim of the study was to evaluate whether hUSCs have protective effect on streptozotocin (STZ)-induced diabetes. hUSCs were extracted and cultivated with a special culture medium. Flow cytometry analysis was applied to detect cell surface markers. BALB/c male nude mice were either injected with high-dose STZ (HD-STZ) or multiple low-dose STZ (MLD-STZ)...
April 19, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29667149/three-dimensional-reconstructed-eccrine-sweat-glands-with-vascularization-and-cholinergic-and-adrenergic-innervation
#6
Mingjun Zhang, Haihong Li, Liyun Chen, Shuhua Fang, Sitian Xie, Changmin Lin
Functional integrity of the regenerated tissues requires not only structural integrity but also vascularization and innervation. We previously demonstrated that the three-dimensional (3D) reconstructed eccrine sweat glands had similar structures as those of the native ones did, but whether the 3D reconstructed glands possessing vascularization and innervation was still unknown. In the study, Matrigel-embedded eccrine sweat gland cells were implanted under the inguinal skin. Ten weeks post-implantation, the vascularization, and innervation in the 10-week reconstructed eccrine sweat glands and native human eccrine sweat glands were detected by immunofluorescence staining...
April 17, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29644561/osteoprotegerin-deficiency-causes-morphological-and-quantitative-damage-in-epithelial-rests-of-malassez
#7
Yunfei Wang, Mengmeng Liu, Shijian Deng, Xin Sui, Linlin Fan, Qi Zhang
Epithelial rests of Malassez (ERM), the only odontogenic epithelial structures in periodontal tissue, are proposed to correlate with root resorption, but the detailed mechanism remains unclear. Osteoprotegerin (OPG), the main inhibitor of osteoclastogenesis, plays a pivotal role in inhibiting root resorption, and ERM cells express OPG mRNA in vitro. Thus, in this study, we aimed to clarify OPG expression in ERM in vivo and to explore the role of OPG in ERM to determine whether ERM are associated with root resorption via OPG...
April 11, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29637382/effects-of-cd100-promote-wound-healing-in-diabetic-mice
#8
Fang Wang, Bei Liu, Zhou Yu, Tong Wang, Yajuan Song, Ran Zhuang, Yonghong Wu, Yingjun Su, Shuzhong Guo
Diabetes is a condition that causes delayed wound healing and results in chronic wounds. CD100 has been reported to promote and induce potent and obvious angiogenesis both in vivo and in vitro studies, the absence of which are a main cause of the diabetic chronic wound. In the present study, we investigated the effects of application of soluble CD100 on wound healing in diabetic mice. Four 5-mm full-thickness dermal wounds were made on each male db/db mouse. 12 mice from CD100 group were subcutaneously injected with 250 ng of CD100 (50 µl) per wound, in addition, 12 mice were injected with the same volume phosphate-balanced solution as the control...
April 10, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29633149/human-nail-stem-cells-are-retained-but-hypofunctional-during-aging
#9
Jia Shi, Zhengtao Lv, Mingbo Nie, Weiwei Lu, Changyu Liu, Yong Tian, Long Li, Guoxiang Zhang, Ranyue Ren, Ziyang Zhang, Hao Kang
The nail is a continuous skin appendage. Cells located around the nails, which display coordinated homeostatic dynamics and release a flow of stem cells in response to regeneration, have been identified in mice. However, very few studies regarding human nail stem cells exist in the literature. Using specimens isolated from humans, we detected an unreported population of cells within the basal layer of postnatal human nail proximal folds (NPFs) and the nail matrix around the nail root. These cells were multi-expressing and expressed stem cell markers, such as keratin 15 (K15), keratin 14 (K14), keratin 19 (K19), CD29, CD34, and leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6)...
April 9, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29603045/wnt-%C3%AE-catenin-signaling-pathway-in-severe-preeclampsia
#10
Xiaofang Wang, Zhan Zhang, Xianxu Zeng, Jinming Wang, Linlin Zhang, Wanyu Song, Ying Shi
This study aims to elucidate the mechanisms of Wnt/β-catenin signaling pathway in the development of preeclampsia (PE). The mRNA levels of Wnt1, β-catenin, c-myc and cyclinD1 were determined by real-time PCR in the placentas. Moreover, the expression levels of Wnt1, β-catenin, Dickkopf-1 (DKK1) and glycogen synthase kinase 3β (GSK-3β) proteins were detected by Western blot. Immunohistochemistry was used in placental tissue microarray to localize the expression of Wnt1, β-catenin, DKK1 proteins in the placentas of two groups...
March 30, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29569168/development-of-decellularized-amniotic-membrane-as-a-bioscaffold-for-bone-marrow-derived-mesenchymal-stem-cells-ultrastructural-study
#11
Radwa Ayman Salah, Ihab K Mohamed, Nagwa El-Badri
Developing effective stem cell-based therapies requires the design of complex in vitro culture systems for accurate representation of the physiological stem cell niche. Human amniotic membrane (hAM) has been successfully used in clinical grafting applications due to its unique biological and regenerative properties. Decellularized hAM (d-hAM) has been previously applied to the culture of human bone marrow mesenchymal stem cells (hMSCs), promoting their expansion and differentiation into adipogenic and osteogenic lineages...
March 22, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29525888/effect-of-preameloblast-conditioned-medium-and-cpne7-on-root-surfaces-in-dogs-a-histologic-and-histomorphometric-evaluation
#12
Sang-Joun Yu, Yoon Seon Lee, Han-Wool Choung, Yeoung-Hyun Park, Byung-Ock Kim, Joo-Cheol Park
Preameloblast-conditioned medium (PACM) has been reported as a potent dentin regenerative material, but its effects as a mixture on periodontal regeneration and the role of CPNE7 in PACM are not known. The purpose of this study is to evaluate the histologic and histomorphometric effects of preameloblast-conditioned medium (PACM) and CPNE7 on periodontal tissue healing in dogs. Seventy-two mandibular premolar roots from ten dogs were extracted and randomly divided into six groups (n = 12 each): (1) positive control group; (2) negative control group; (3) cementum-removed and PACM-treated group; (4) cementum-preserved and PACM-treated group; (5) CPNE7-inactivated PACM-treated group; and (6) recombinant CPNE7-treated group...
June 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29516260/activation-of-satellite-glial-cells-in-trigeminal-ganglion-following-dental-injury-and-inflammation
#13
Haichao Liu, Lei Zhao, Wenzhen Gu, Qin Liu, Zhixiong Gao, Xiao Zhu, Zhi Wu, Hongwen He, Fang Huang, Wenguo Fan
Satellite glial cells (SGCs), a peripheral neuroglial cell, surround neurons and form a complete envelope around individual sensory neurons in the trigeminal ganglia (TG), which may be involved in modulating neurons in inflammation. The purpose of this study was to determine the effect of dental injury and inflammation on SGCs in the TG. Pulp exposure (PX) was performed on the first maxillary molar of 28 rats. The neurons innervating injured tooth in TG were labeled by the retrograde transport of fluoro-gold (FG)...
June 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29468299/smad4-and-epithelial-mesenchymal-transition-proteins-in-colorectal-carcinoma-an-immunohistochemical-study
#14
M Ioannou, E Kouvaras, R Papamichali, M Samara, I Chiotoglou, G Koukoulis
Epithelial-mesenchymal transition (EMT) plays an important role in cancer metastasis. During EMT, tumor cells acquire the capacity to migrate and invade the stroma. Activation of the transforming growth factor-b (TGF-b) signaling pathway is of major importance for the initiation of EMT. Smad4, an essential protein of this pathway, is known to complex with multiple transcription factors (e.g. Snail-1, Slug, Twist-1), in various types of cancer, promoting the repression or activation of target genes. The role of Smad4 in colorectal cancer (CRC) is not straightforward so far...
June 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29468298/endoscopy-histology-and-electron-microscopy-analysis-of-foetal-membranes-in-pregnant-south-american-plains-vizcacha-reveal-unusual-excrescences-on-the-yolk-sac
#15
Mariela Giacchino, Pablo I F Inserra, Fernando D Lange, María C Gariboldi, Sergio R Ferraris, Alfredo D Vitullo
The South American hystricognathe Lagostomus maximus is a fossorial rodent whose females show unique reproductive characteristics. They have a 155-day long gestation, show massive polyovulation and a selective process of embryonic resorption in the first half of gestation. In order to explore and perform an in-situ characterization of the reproductive tract, we visualized internal structures through ultrasonography and video-endoscopy in pregnant and non-pregnant females. We describe the finding of protruding structures that lie on the yolk sac and their histological and ultrastructural characterization...
June 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29480405/mir-484-map2-c-myc-positive-regulatory-loop-in-glioma-promotes-tumor-initiating-properties-through-erk1-2-signaling
#16
Renhui Yi, Jiugeng Feng, Shaochun Yang, Xiaoyu Huang, Yuanyuan Liao, Zheng Hu, Muyun Luo
Glioma is the most common intracranial malignant tumor. Cancer stem cells (CSCs) are resistant to chemotherapy and radiotherapy, and are closely related to cancer metastasis and recurrence. In this study, we aimed to explore the effect of miR-484 on glioma stemness and the underlying mechanism involved. miR-484 enhanced glioma tumor-initiating properties in vitro and in vivo. Moreover, miR-484 was shown to directly target MAP2, resulting in activation of ERK1/2 signaling and promotion of stemness in glioma...
April 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29455276/methylation-of-secreted-frizzled-related-protein-1-sfrp1-promoter-downregulates-wnt-%C3%AE-catenin-activity-in-keloids
#17
Jiaqi Liu, Huayu Zhu, Hongtao Wang, Jun Li, Fu Han, Yang Liu, Wanfu Zhang, Ting He, Na Li, Zhao Zheng, Dahai Hu
Keloid, a benign skin disorder, forms during wound healing in genetically susceptible individuals. To better control keloid and understand the molecular mechanisms, this study screened gene hypermethylations of GEO database microarray data on keloids and identified the hypermethylation of the secreted frizzled related protein-1 (SFRP1) promoter. Subsequently, hypermethylation and mRNA and protein levels were assessed in 57 cases of keloid vs. normal skin tissues. Fibroblasts from tissues were isolated for the assessment of gene regulation in vitro...
April 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29453757/localization-of-epithelial-sodium-channel-enac-and-cftr-in-the-germinal-epithelium-of-the-testis-sertoli-cells-and-spermatozoa
#18
Sachin Sharma, Aaron Hanukoglu, Israel Hanukoglu
Spermatogenesis starts within the seminiferous tubules of the testis by mitotic division of spermatogonia that produces spermatocytes. Meiotic division of these spermatocytes produces haploid spermatids that differentiate into spermatozoa. In this study, we examined the expression of ENaC and CFTR (a Cl- channel) in rat testicular sections using confocal microscopic immunofluorescence. The structural integrity of the seminiferous tubule sections was verified by precise phalloidin staining of the actin fibers located abundantly at both basal and adluminal tight junctions...
April 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29442209/capillary-rarefaction-and-altered-renal-development-the-imbalance-between-pro-and-anti-angiogenic-factors-in-response-to-angiotensin-ii-inhibition-in-the-developing-rat-kidney
#19
Kee Hwan Yoo, Hyung Eun Yim, Eun Soo Bae, Young Sook Hong
Proper and timely assembly of the kidney vasculature with their respective nephrons is crucial during normal kidney development. In this study, we investigated the effects of enalapril (angiotensin-converting enzyme inhibitor) on angiogenesis-related gene expression and microvascular endothelium related to glomeular and tubular changes in the neonatal rat kidney. Enalapril-treated rats had higher tubular injury scores and lower glomerular maturity grades than those of untreated rats. In the enalapril-treated group, intrarenal angiopoietin-2, Tie-2, and thrombospondin-1 protein expression increased, whereas intrarenal angiopoietin-1 protein expression decreased...
April 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29372370/slc30a-family-expression-in-the-pancreatic-islets-of-humans-and-mice-cellular-localization-in-the-%C3%AE-cells
#20
Yimeng Cai, Catherine P Kirschke, Liping Huang
Zinc is a vital co-factor for insulin metabolism in the pancreatic β-cell, involved in synthesis, maturation, and crystallization. Two families of zinc transporters, namely SLC30A (ZNT) and SLC39A (ZIP) are involved in maintaining cellular zinc homeostasis in mammalian cells. Single nuclear polymorphisms or mutations in zinc transporters have been associated with insulin resistance and risk of type 2 diabetes (T2D) in both humans and mice. Thus, mice can be useful for studying the underlying mechanisms of zinc-associated risk of T2D development...
April 2018: Journal of Molecular Histology
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