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Journal of Molecular Histology

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https://www.readbyqxmd.com/read/29119279/mechanical-stress-promotes-matrix-synthesis-of-mandibular-condylar-cartilage-via-the-rkip-erk-pathway
#1
Lian Sun, Jing Zhao, Hua Wang, Yongchu Pan, Lin Wang, Wei-Bing Zhang
Mandibular hypoplasia is a common jaw deformity that affects breathing, occlusal function and facial aesthetics. Stimulating mandibular condylar growing with functional appliances is an ordinary but controversial treatment method in orthodontics. Therefore, it is vital to clarify how functional appliances affect condylar growing. Raf-1 kinase inhibitor protein (RKIP), as an endogenous inhibitory molecule of the ERK signaling, is postulated to involve in stress-induced response to articular cartilage. This study was to reveal the role of RKIP in regulating cartilage matrix synthesis with functional appliance treatment...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29094227/down-regulation-of-noggin-and-mir-138-coordinately-promote-osteogenesis-of-mesenchymal-stem-cells
#2
Xing-Kun Sun, Jin Zhou, Lei Zhang, Tian Ma, Yu-Han Wang, Yan-Mei Yang, Yan-Ting Tang, Hong Li, Li-Jun Wang
Mesenchymal stem cells (MSCs) can differentiate to osteocytes under suitable conditions. In recent years, micro-nucleotides have been progressively used to modulate gene expression in cells due to the consideration of safety. Our present study aimed to investigate whether co-delivery of Noggin-siRNA and antimiR-138 enhances the osteogenic effect of MSCs. Using a murine MSC line, C3H/10T1/2 cells, the delivery efficiency of Noggin-siRNA and antimiR-138 into MSCs was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR)...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29027601/dnmt1-dnmt3a-and-dnmt3b-proteins-are-differently-expressed-in-mouse-oocytes-and-early-embryos
#3
Fatma Uysal, Saffet Ozturk, Gokhan Akkoyunlu
DNA methylation is one of the epigenetic mechanisms and plays important roles during oogenesis and early embryo development in mammals. DNA methylation is basically known as adding a methyl group to the fifth carbon atom of cytosine residues within cytosine-phosphate-guanine (CpG) and non-CpG dinucleotide sites. This mechanism is composed of two main processes: de novo methylation and maintenance methylation, both of which are catalyzed by specific DNA methyltransferase (DNMT) enzymes. To date, six different DNMTs have been characterized in mammals defined as DNMT1, DNMT2, DNMT3A, DNMT3B, DNMT3C, and DNMT3L...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28988314/the-prostate-response-to-prolactin-modulation-in-adult-castrated-rats-subjected-to-testosterone-replacement
#4
Flávia B Constantino, Ana C L Camargo, Sérgio A A Santos, Ketlin T Colombelli, Laura F Martin, Marcia G Silva, Sérgio L Felisbino, Luis A Justulin
Despite the androgenic dependence, other hormones, growth factors, and cytokines are necessary to support prostatic growth and maintain the glandular structure; among them, prolactin is a non-steroidal hormone secreted mainly by the pituitary gland. However, extra-pituitary expression of prolactin, such as in the prostate, has also been demonstrated, highlighting the paracrine and autocrine actions of prolactin within the prostate. Here, we investigated whether prolactin modulation alters ventral prostate (VP) morphophysiology in adult castrated rats...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28986711/blockade-of-lgr4-inhibits-proliferation-and-odonto-osteogenic-differentiation-of-stem-cells-from-apical-papillae
#5
Meng Zhou, Shuyu Guo, Lichan Yuan, Yuxin Zhang, Mengnan Zhang, Huimin Chen, Mengting Lu, Jianrong Yang, Junqing Ma
During tooth root development, stem cells from apical papillae (SCAPs) are indispensable, and their abilities of proliferation, migration and odontoblast differentiation are linked to root formation. Leucine-rich repeat-containing GPCR 4 (LGR4) modulates the biological processes of proliferation and differentiation in multiple stem cells. In this study, we showed that LGR4 is expressed in all odontoblast cell lineage cells and Hertwig's epithelial root sheath (HERS) during the mouse root formation in vivo. In vitro we determined that LGR4 is involved in the Wnt/β-catenin signaling pathway regulating proliferation and odonto/osteogenic differentiation of SCAPs...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28975535/spatiotemporal-expression-of-proprotein-convertase-subtilisin-kexin-type-5-in-the-development-of-anorectal-malformations-in-fetal-rats
#6
Xiao Bing Tang, Li Chen, Wei Lin Wang, Zheng Wei Yuan, Yu Zuo Bai
This study examined the expression patterns of proprotein convertase subtilisin/kexin type 5 (Pcsk5) during anorectal development in normal and anorectal malformations (ARM) rat embryos, determine the possible role of Pcsk5 in the pathogenesis of ARM. An ARM rat model was developed by the administration of ethylenethiourea gestational day 10 (GD10). Embryos were harvested by surgical excision from GD13 to GD16, and the spatiotemporal expression of Pcsk5 was evaluated, using immunohistochemistry staining, Western blotting and real time RT-PCR...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28905239/expression-and-distribution-of-three-transient-receptor-potential-vanilloid-trpv-channel-proteins-in-human-odontoblast-like-cells
#7
Wen Wen, Kehua Que, Chengcheng Zang, Jing Wen, Guangxu Sun, Zhiying Zhao, Yanzhong Li
Odontoblasts have been suggested to contribute to nociceptive sensation in the tooth via expression of the transient receptor potential (TRP) channels. The TRP channels as a family of nonselective cation permeable channels play an important role in sensory transduction of human. In this study, we examined the expression of transient receptor potential vanilloid-1 (TRPV1), transient receptor potential vanilloid-2 (TRPV2) and transient receptor potential vanilloid-3 (TRPV3) channels in native human odontoblasts (HODs) and long-term cultured human dental pulp cells with odontoblast phenotyoe (LHOPs) obtained from healthy wisdom teeth with the use of immunohistochemistry (IHC), immunofluorescence (IF), quantitative real-time polymerase chain reaction (qRT-PCR),western blotting (WB) and immunoelectron microscopy (IEM) assay...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28884252/microrna-192-regulates-hypertrophic-scar-fibrosis-by-targeting-sip1
#8
Yan Li, Julei Zhang, Wei Zhang, Yang Liu, Yuehua Li, Kejia Wang, Yijie Zhang, Chen Yang, Xiaoqiang Li, Jihong Shi, Linlin Su, Dahai Hu
Hypertrophic scar (HS) is a fibro-proliferative disorder which is characterized by excessive deposition of collagen and accumulative activity of myofibroblasts. Increasing evidences have demonstrated miRNAs play a pivotal role in the pathogenesis of HS. MiR-192 is closely associated with renal fibrosis, but its effect on HS formation and skin fibrosis remains unknown. In the study, we presented that miR-192 was up-regulated in HS and HS derived fibroblasts (HSFs) compared to normal skin (NS) and NS derived fibroblasts (NSFs), accompanied by the reduction of smad interacting protein 1 (SIP1) expression and the increase of Col1, Col3 and α-SMA levels...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28825193/differential-expression-of-transforming-growth-factor-beta1-connective-tissue-growth-factor-phosphorylated-smad2-3-and-phosphorylated-erk1-2-during-mouse-tooth-development
#9
Shubo Li, Yihuai Pan
Connective tissue growth factor (CTGF) is a downstream mediator of transforming growth factor-beta 1 (TGF-β1) and TGF-β1-induced CTGF expression is regulated through SMAD and mitogen-activated protein kinase (MAPK) signaling pathways. The fine modulation of TGF-β1 signaling is very important to the process of tooth development. However, little is known about the localization of CTGF, MAPK and SMAD in the context of TGF-β1 signaling during odontogenesis. Hence, we aimed to investigate the expression of TGF-β1, CTGF, phosphorylated-SMAD2/3 (p-SMAD2/3) and phosphorylated-ERK1/2 (p-ERK1/2)...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28756608/inhibition-of-chondroitin-sulfate-glycosaminoglycans-incorporation-affected-odontoblast-differentiation-in-cultured-embryonic-mouse-molars
#10
Lipei Liu, Weiting Chen, Lefeng Li, Fangfang Xu, Beizhan Jiang
Chondroitin sulfate proteoglycan (CSPG) is an important component of extracellular matrix (ECM), it is composed of a core protein and one or more chondroitin sulfate glycosaminoglycan side chains (CS-GAGs). To investigate the roles of its CS-GAGs in dentinogenesis, the mouse mandibular first molar tooth germs at early bell stage were cultivated with or without β-xyloside. As expected, the CS-GAGs were inhibited on their incorporation to CSPGs by β-xyloside, accompanied by the change of morphology of the cultured tooth germs...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28766180/the-expression-of-water-channel-proteins-during-human-salivary-gland-development-a-topographic-study-of-aquaporins-1-3-and-5
#11
Fernanda de Paula, Tathyane Harumi Nakajima Teshima, Ricardo Hsieh, Milena Monteiro Souza, Claudia Malheiros Coutinho-Camillo, Marcello Menta Simonsen Nico, Silvia Vanessa Lourenco
Some members of aquaporin family (AQP) plays crucial functions in salivary synthesis and secretion. These proteins expression has already been reported during salivary gland formation, however no previous studies in human developing glands have been performed. We evaluated AQP1, 3 and 5 expression through the stages of human salivary gland morphogenesis and discuss the possible role of AQP for glandular maturation. Human salivary glands derived from foetuses aged between 14 and 25 weeks were submitted to immunohistochemistry...
August 1, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28687932/spatial-and-temporal-expression-of-sox9-during-murine-incisor-development
#12
Zhi-Cheng Yang, Dan Li, Shuang Feng, Xiao-Dong Sui, Zhi Chen, Li Zhang
Mouse incisors are capable of continuously growing due to the renewal of dental epithelium stem cells and mesenchymal stem cells residing at the proximal ends. The transcription factor Sox9 plays important roles in maintaining the stem cells of hair follicles, retinal progenitor cells and neural crest stem cells. Whether Sox9 is involved during mouse incisor development is not reported yet. In this study, we examined the expression pattern of Sox9 during mouse incisor development by in situ hybridization and immunohistochemistry...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28647773/cthrc1-promotes-osteogenic-differentiation-of-periodontal-ligament-stem-cells-by-regulating-taz
#13
Chengze Wang, Weiting Gu, Baiyu Sun, Yunpeng Zhang, Yawen Ji, Xin Xu, Yong Wen
Collagen triple helix repeat containing 1 (CTHRC1) is associated with bone metabolism. Alveolar bone has an ability to rapidly remodel itself to adapt its biomechanical environment and function. However, whether CTHRC1 is expressed in alveolar bone tissue and the role of CTHRC1 in alveolar bone remodeling remain unclear. We used orthodontic tooth movement (OTM) rat model to study the effects of CHTRC1 in alveolar bone remodeling in vivo. We found that CTHRC1 was expressed in normal physiological condition of osteocytes, bone matrix, and periodontal ligament cells in rat...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28643114/synergistic-cardioprotective-effects-of-raav9-cyclina2-combined-with-fibrin-glue-in-rats-after-myocardial-infarction
#14
Wen Cao, Ya-Fei Chang, Ai-Chao Zhao, Bang-Dang Chen, Fen Liu, Yi-Tong Ma, Xiang Ma
The present study aimed to investigate the protective effects of rAAV9-CyclinA2 combined with fibrin glue (FG) in vivo in rats after myocardial infarction (MI). Ninety male Sprague-Dawley rats were randomized into 6 groups (15 in each group): sham, MI, rAAV9-green fluorescent protein (GFP) + MI, rAAV9-CyclinA2 + MI, FG + MI, and rAAV9-CyclinA2 + FG + MI. Packed virus (5 × 10(11)vg/ml) in 150 µl of normal saline or FG was injected into the infarcted myocardium at five locations in rAAV9-GFP + MI, rAAV9-CyclinA2 + MI, and rAAV9-CyclinA2 + FG + MI groups...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28597201/methane-alleviates-carbon-tetrachloride-induced-liver-injury-in-mice-anti-inflammatory-action-demonstrated-by-increased-pi3k-akt-gsk-3%C3%AE-mediated-il-10-expression
#15
Ying Yao, Liping Wang, Peipei Jin, Na Li, Yan Meng, Changli Wang, Mengda Xu, Yan Zhang, Jinjun Bian, Xiaoming Deng
The inflammatory response plays an important role in carbon tetrachloride (CCl4)-induced acute liver injury and methane has been shown to exert beneficial effects on inflammation-associated diseases. Thus, we investigated the potential protective effects of methane-rich saline (MS) on CCl4-induced acute liver injury and explored the underlying mechanism. A CCl4-induced acute liver injury model was established by injection of CCl4 (0.6 ml/kg, ip) in mice followed by treatment with MS (16 ml/kg, ip), 24 h later...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28550642/post-heroin-dose-tissue-distribution-of-6-monoacetylmorphine-6-mam-with-maldi-imaging
#16
Belin G Teklezgi, Annapurna Pamreddy, Sooraj Baijnath, Nirmala D Gopal, Tricia Naicker, Hendrik G Kruger, Thavendran Govender
Heroin is an illicit opioid drug which is commonly abused and leads to dependence and addiction. Heroin is considered a pro-drug and is rapidly converted to its major active metabolite 6-monoacetylmorphine (6-MAM) which mediates euphoria and reward through the stimulation of opioid receptors in the brain. The aim of this study was to investigate the distribution and localization of 6-MAM in the healthy Sprague Dawley rat brain following intraperitoneal (i.p) administration of heroin (10 mg/kg), using matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI), in combination with quantification via liquid chromatography mass spectrometry (LC-MS/MS)...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28547585/aio-casilio-a-robust-crispr-cas9-pumilio-system-for-chromosome-labeling
#17
Shuxian Zhang, Zhan Song
The biological function of chromatin bases on its spatial organization and dynamic activities in different situations. Labeling and tracing of genomic sequences has been a huge challenge in studying the spatial dynamics of chromatin. We reported the development of 'all-in-one Casilio system (Aio-Casilio)', a new system that enables the labeling of endogenous genomic loci. The Aio-Casilio system consists of the dCas9 protein, mClover fused with the Pumilio and FBF proteins RNA-binding domain (PUF domain) and an U6-sgRNA appended with multiple PUF-binding site(s)...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28455549/involution-processes-of-follicular-atresia-and-post-ovulatory-complex-in-a-characid-fish-ovary-a-study-of-apoptosis-and-autophagy-pathways
#18
Mônica Cassel, Marília de Paiva Camargo, Lázaro Wender Oliveira de Jesus, Maria Inês Borella
Recent studies seem to indicate that apoptosis and autophagy can act cooperatively in fish ovaries in order to achieve more effective ovarian regression after spawning. Considering the importance of tissue remodeling in ovarian functioning, we sought to morphologically characterize the involution processes of follicular atresia and post-ovulatory complexes using Astyanax altiparanae as an experimental model, and to determine the location of proteins involved in apoptosis and autophagy throughout this process...
June 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28451773/correlation-between-three-dimensional-power-doppler-and-morphometric-measurement-of-endometrial-vascularity-at-the-time-of-embryo-implantation-in-women-with-unexplained-recurrent-miscarriage
#19
Xiaoyan Chen, Sotirios H Saravelos, Yingyu Liu, Jin Huang, Chi Chiu Wang, Tin Chiu Li
Power Doppler in combination with three-dimensional (3D-PD) ultrasonography has been used as a noninvasive tool to evaluate the vascularity. However, it is unclear whether 3D-PD can accurately reflect endometrial vascularization and replace the invasive endometrial biopsy. This study aims to investigate the correlation between 3D-PD and micro vessel morphometric measurement of endometrial vascularity. Twenty-five women with unexplained recurrent miscarriage were recruited for 3D-PD and endometrial biopsy on precisely day LH + 7...
June 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28409326/effect-of-corticision-on-orthodontic-tooth-movement-in-a-rat-model-as-assessed-by-rna-sequencing
#20
Qihui Gu, Shuyu Guo, Dongyue Wang, Tingting Zhou, Lin Wang, Zhendong Wang, Junqing Ma
Corticision is a common technique to accelerate orthodontic tooth movement; however, not much is known about the underlying mechanisms. In this study, we investigated the mechanism of alveolar tissue remodeling after corticision in a rat model of tooth movement (TM) by analyzing the differential transcriptome. A total of 36 male rats were equally divided into TM and TM with corticision (TM+C) groups. Alveolar bone response was examined using micro-computed tomography (micro-CT). Osteoclasts and osteoblasts were quantified on tartrate-resistant acid phosphatase (TRAP) and Goldner's trichrome staining...
June 2017: Journal of Molecular Histology
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