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Journal of Molecular Histology

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https://www.readbyqxmd.com/read/28093664/cell-proliferation-and-differentiation-during-the-three-dimensional-reconstitution-of-eccrine-sweat-glands
#1
Xuexue Li, Haihong Li, Mingjun Zhang, Liyun Chen, Bingna Zhang
The aim of this study is to characterize the cell proliferation and proliferating cell types during three-dimensional reconstitution of eccrine sweat glands. Eccrine sweat gland cells suspended in Matrigel were injected subcutaneously into the inguinal regions of nude mice. At 1, 2, 4, 6, 8, 14, 21, 28, 35 and 42 days post-implantation, Matrigel plugs were immunostained for Ki67, to detect cycling cells, and the Ki67 labeling index at different time points was calculated. Three pairs of antibodies, Ki67/K7, Ki67/K14 and Ki67/α-SMA, were used to identify proliferating cell types in the plugs, on days 28, 35 and 42, by immunofluorescence double staining...
January 16, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28078480/muscarinic-acetylcholine-receptor-mediated-tight-junction-opening-is-involved-in-epiphora-in-late-phase-of-submandibular-gland-transplantation
#2
Ning-Yan Yang, Chong Ding, Jing Li, Yan Zhang, Ruo-Lan Xiang, Li-Ling Wu, Guang-Yan Yu, Xin Cong
Submandibular gland (SMG) autotransplantation is an effective therapy for treating severe dry eye syndrome. However, epiphora occurs in more than 40% of patients 6 months after operation. We previously found that muscarinic acetylcholine receptor (mAChR) plays a crucial role in regulating SMG secretion partially through the modulation on tight junction (TJ)-based paracellular pathway. Therefore, the present study aimed to investigate the possible involvement of mAChR and TJ in a rabbit long-term model of SMG transplantation...
January 11, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28000152/inactivation-of-bone-morphogenetic-protein-1-bmp1-and-tolloid-like-1-tll1-in-cells-expressing-type-i-collagen-leads-to-dental-and-periodontal-defects-in-mice
#3
Hua Zhang, Priyam Jani, Tian Liang, Yongbo Lu, Chunlin Qin
Bone morphogenetic protein 1 (BMP1) and tolloid-like 1 (TLL1) belong to the BMP1/tolloid-like proteinase family, which cleaves secretory proteins. The constitutive deletion of the Bmp1 or Tll1 genes causes perinatal or embryonic lethality in mice. In this study, we first studied the β-galactosidase activity in mice in which an IRES-lacZ-Neo cassette was inserted in the intron of either the Bmp1 or the Tll1 gene; the β-galactosidase activities were used to reflect the expression of endogenous Bmp1 and Tll1, respectively...
December 20, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27981392/histological-evidence-that-metformin-reverses-the-adverse-effects-of-diabetes-on-orthodontic-tooth-movement-in-rats
#4
Jing Sun, Juan Du, Wei Feng, Boyao Lu, Hongrui Liu, Jie Guo, Norio Amizuka, Minqi Li
This study evaluated the effects of metformin on orthodontic tooth movement in a rat model of type 2 diabetes mellitus. Rats were fed a high-fat diet for 4 weeks to induce fat accumulation and insulin resistance, and then injected with a low dose of streptozotocin (35 mg/kg) intraperitoneally to induce type 2 diabetes. An orthodontic appliance was placed in normoglycemic, type 2 diabetes, and type 2 diabetes with metformin-administrated rats. After 14 days, type 2 diabetes rats exhibited greater orthodontic tooth movement and had a higher number of tartrate-resistant acid phosphatase-positive osteoclasts, stronger cathepsin K expression, and weaker alkaline phosphatase immunostaining than normoglycemic rats...
December 15, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27913976/follistatin-like-protein-1-suppressed-pro-inflammatory-cytokines-expression-during-neuroinflammation-induced-by-lipopolysaccharide
#5
Kai-Yuan Cheng, Yi Liu, Ying-Guang Han, Jing-Kun Li, Jia-Lin Jia, Bin Chen, Zhi-Xiao Yao, Lin Nie, Lei Cheng
Follistain-like protein 1 (FSTL1), has been recently demonstrated to be involved in the embryo development of nervous system and glioblastoma. However, the role of FSTL1 in neuroinflammation remains unexplored. In this study, the expression of FSTL1 in astrocytes was verified and its role was studied in neuroinflammation induced by in vivo intracerebroventricular (ICV) injection of lipopolysaccharide (LPS) or LPS treatment to astrocytes in vitro. FSTL1 was significantly induced after ICV LPS injection or LPS treatment...
December 2, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27885497/erratum-to-a-zebrafish-mosaic-assay-to-study-mammalian-stem-cells-in-real-time-in-vivo
#6
Chun Xiao, Meilin Qian, Chaoran Yin, Yonggang Zhang, Huozhen Hu, Shaohua Yao
No abstract text is available yet for this article.
November 24, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27882438/histochemical-examination-of-systemic-administration-of-eldecalcitol-combined-with-guided-bone-regeneration-for-bone-defect-restoration-in-rats
#7
Xiuchun Han, Juan Du, Di Liu, Hongrui Liu, Norio Amizuka, Minqi Li
The aim of this experiment was to elucidate the histological alterations after systemic administration of eldecalcitol (ELD) combined with guided bone regeneration during the restoration of bone defect healing in rats. The femurs of 8-week-old Wister rats were used to generate bone defect models. The defect was covered with a collagen membrane, and ELD group was administrated with eldecalcitol (50 ng/kg body weight) intragastrically once every other day. Femora were harvested at 1, 2, 4 and 8 weeks post-surgery...
November 23, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27722791/a-modified-protocol-for-the-detection-of-three-different-mrnas-with-a-new-generation-in-situ-hybridization-chain-reaction-on-frozen-sections
#8
Qian-Qian Sui, Jiao Zhu, Xiangnan Li, Gillian E Knight, Cheng He, Geoffrey Burnstock, Hongbin Yuan, Zhenghua Xiang
A new multiple fluorescence in situ hybridization method based on hybridization chain reaction was recently reported, enabling simultaneous mapping of multiple target mRNAs within intact zebrafish and mouse embryos. With this approach, DNA probes complementary to target mRNAs trigger chain reactions in which metastable fluorophore-labeled DNA hairpins self-assemble into fluorescent amplification polymers. The formation of the specific polymers enhances greatly the sensitivity of multiple fluorescence in situ hybridization...
December 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27714564/heat-shock-protein-27-hsp27-regulates-stat3-and-eif4g-levels-in-first-trimester-human-placenta
#9
Gali Epstein Shochet, Oded Komemi, Dana Sadeh-Mestechkin, Meir Pomeranz, Ami Fishman, Liat Drucker, Michael Lishner, Shelly Tartakover Matalon
During placental implantation, cytotrophoblast cells differentiate to extravillous trophoblast (EVT) cells that invade from the placenta into the maternal uterine blood vessels. The heat shock protein-27 (HSP27), the signal transducer and activator of transcription-3 (STAT3) and the eukaryotic translation initiation factor 4E (EIF4E) are involved in regulating EVT cell differentiation/migration. EIF4E and EIF4G compose the translation initiation complex, which is a major control point in protein translation...
December 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27696080/determining-protein-biomarkers-for-dlbcl-using-ffpe-tissues-from-hiv-negative-and-hiv-positive-patients
#10
Pumza Magangane, Raveendra Sookhayi, Dhirendra Govender, Richard Naidoo
DLBCL is the most common lymphoma subtype occurring in older populations as well as in younger HIV infected patients. The current treatment options for DLBCL are effective for most patients yet the relapse rate is high. While many biomarkers for DLBCL exist, they are not in clinical use due to low sensitivity and specificity. In addition, these biomarkers have not been studied in the HIV context. Therefore, the identification of new biomarkers for HIV negative and HIV positive DLBCL, may lead to a better understanding of the disease pathology and better therapeutic design...
December 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27681986/age-dependent-impact-of-inferior-alveolar-nerve-transection-on-mandibular-bone-metabolism-and-the-underlying-mechanisms
#11
Qingqing Wu, Bo Yang, Cong Cao, Mengkai Guang, Ping Gong
Aging is associated with peripheral nerve degradation and bone destruction. The aim of the study is to elucidate the influence of sensory denervation on bone metabolism in different age groups by establishing a modified unilateral inferior alveolar nerve transection (IANT) model. The rats, divided into young, middle-aged and aged group, were sacrificed at 1, 2, 4 and 8 weeks after right IANT. The histological changes of mandibles were analyzed by fluorescent double labeling, micro-CT, HE, TRAP and anti-CGRP immunohistochemical staining...
December 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27650519/n-cadherin-regulates-beta-catenin-signal-and-its-misexpression-perturbs-commissural-axon-projection-in-the-developing-chicken-spinal-cord
#12
Ciqing Yang, Xiaoying Li, Congrui Wang, Sulei Fu, Han Li, Zhikun Guo, Shanting Zhao, Juntang Lin
N-cadherin is a calcium-sensitive cell adhesion molecule that plays an important role in the formation of the neural circuit and the development of the nervous system. In the present study, we investigated the function of N-cadherin in cell-cell connection in vitro with HEK293T cells, and in commissural axon projections in the developing chicken spinal cord using in ovo electroporation. Cell-cell connections increased with N-cadherin overexpression in HEK293T cells, while cell contacts disappeared after co-transfection with an N-cadherin-shRNA plasmid...
December 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27566703/the-function-of-sperm-associated-antigen-6-in-neuronal-proliferation-and-differentiation
#13
Xinde Hu, Runchuan Yan, Xinran Cheng, Lingzhen Song, Wei Zhang, Kaikai Li, Shanting Zhao
Sperm-associated antigen 6 (SPAG6) is initially found in human testis and is essential for sperm motility and male fertility. Later studies indicate that it also express in the chick Central Nervous System and human embryonic stem cells. However, the function of Spag6 in cortical development is still largely unclear. Using in utero electroporation, we showed that overexpression of Spag6 induced the transfected cells excluded from the proliferation zone of the mouse cortex. Ki67 Co-labeling and BrdU incorporation experiment suggested that overexpression of Spag6 inhibited proliferation of neural progenitor cells...
December 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27866302/expression-analysis-of-dickkopf-related-protein-3-dkk3-suggests-its-pleiotropic-roles-for-a-secretory-glycoprotein-in-adult-mouse
#14
Junji Inoue, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Hiromi Kumon, Hideyo Ohuchi
Dickkopf-related protein 3 (Dkk3) is the third member of the Dkk gene family and identical to the gene, whose expression was reduced in immortalized cells. Therefore, its another name is reduced expression in immortalized cells. Since the intratumoral introduction of Dkk3 inhibits tumor growth in mouse models of cancers, Dkk3 is likely a tumor suppressor gene. However, the functions of Dkk3 in vivo remain unclear. As the first step to decipher the physiological roles of this gene, we examined the expression pattern of Dkk3 in various tissues from adult mice...
November 19, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27803997/evaluating-the-effect-of-silver-nanoparticles-on-testes-of-adult-albino-rats-histological-immunohistochemical-and-biochemical-study
#15
Samah M Ahmed, Shaimaa A Abdelrahman, Sally M Shalaby
: Silver nanoparticles (AgNPs) are widely used in medicine, however, they have toxic impacts on different organs. AgNPs distribution to the testes was reported, so, we aimed to study the effect of intraperitoneal injection of AgNPs, at different concentrations and different time durations, on adult rat testes. Sixty healthy adult male Wistar albino rats were divided into three groups; control group (Group I) and two experimental groups (Groups II & III), each of which were subdivided into two subgroups...
November 1, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27803996/crim1-is-necessary-for-coronary-vascular-endothelial-cell-development-and-homeostasis
#16
Swati Iyer, Yash Chhabra, Tracey J Harvey, Richard Wang, Han Sheng Chiu, A G Smith, Walter G Thomas, David J Pennisi, Michael Piper
Endothelial cells form a critical component of the coronary vasculature, yet the factors regulating their development remain poorly defined. Here we reveal a novel role for the transmembrane protein CRIM1 in mediating cardiac endothelial cell development. In the absence of Crim1 in vivo, the coronary vasculature is malformed, the number of endothelial cells reduced, and the canonical BMP pathway dysregulated. Moreover, we reveal that CRIM1 can bind IGFs, and regulate IGF signalling within endothelial cells...
November 1, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27787633/foxa1-gene-and-protein-in-developing-rat-eccrine-sweat-glands
#17
Haihong Li, Liyun Chen, Mingjun Zhang, Bingna Zhang
To investigate the development of eccrine sweat glands and the expression of Foxa1 genes and proteins in the course of development, the footpads from E15.5 to E21.5, P1-P12, P14, P21, P28 and P56 rats were subjected to immunofluorescence staining of FoxA1 and double immunofluorescence staining of K14/α-SMA, FoxA1/K7 and FoxA1/α-SMA, and were processed for Foxa1 gene detection by RT-qPCR. The results showed that rat eccrine sweat gland germs was first observed emerging from the basal layer of epidermis at E19...
October 27, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27558143/hyperlipidemia-induced-by-high-fat-diet-enhances-dentin-formation-and-delays-dentin-mineralization-in-mouse-incisor
#18
Xin Ye, Jin Zhang, Pishan Yang
Dyslipidemia has become a serious health problem in children and adolescents worldwide for its high prevalence. Since hard tissues of permanent teeth form mainly during this period and lipids are actively involved in tooth development, the effects of hyperlipidemia on dental tissue formation and mineralization need to be illustrated. In this study, hyperlipidemia model was established in mice fed with high-fat diet (HFD). Micro-CT and histomorphological analyses were performed on the mandibular bones to assess the morphological changes of the mandibular incisor and first molar...
October 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27554369/a-zebrafish-mosaic-assay-to-study-mammalian-stem-cells-in-real-time-in-vivo
#19
Chun Xiao, Meilin Qian, Chaoran Yin, Yonggang Zhang, Huozhen Hu, Shaohua Yao
The differentiation potentials of stem cells have been evaluated by various in vivo and in vitro assays. However, these assays have different limitations hindering efficient study of mammalian stem cells. Here we describe a rapid and powerful mosaic assay to study the differentiation potentials of stem cells in real time in vivo by using zebrafish embryo. We transplanted mouse neural stem cells into zebrafish embryos at different developmental stages and found that they mainly formed neural tissues while occasionally trans-differentiated into mesoderm- and endoderm-derived tissues...
October 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27541036/ovarian-follicular-dynamics-after-aromatizable-or-non-aromatizable-neonatal-androgenization
#20
Gabriel Anesetti, Rebeca Chávez-Genaro
The effects of neonatal testosterone or dihydrotestosterone exposure on ovarian follicular dynamics were analysed at prepubertal, pubertal or adult age in Wistar rats. Both androgens induced a transitory increase on follicular endowment that was partially corrected at puberty. At adult age testosterone prevented ovulation, without significant modifications on follicular dynamics. An increased number of cystic structures were observed from puberty to adult age. However, ovaries of rats treated with dihydrotestosterone showed follicles with evident morphological alterations in granulosa and thecal layers although several corpora lutea were observed...
October 2016: Journal of Molecular Histology
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