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Journal of Molecular Histology

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https://www.readbyqxmd.com/read/28343338/expression-of-typical-osteoclast-markers-by-pbmcs-after-peg-induced-fusion-as-a-model-for-studying-osteoclast-differentiation
#1
Luz M Castillo, Carlos A Guerrero, Orlando Acosta
Bone is a metabolically active organ subjected to continuous remodeling process that involves resorption by osteoclast and subsequent formation by osteoblasts. Osteoclast involvement in this physiological event is regulated by macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor κB ligand (RANKL). Fusion of mono-nuclear pre-osteoclasts is a critical event for osteoclast differentiation and for bone resorption. Here we show that PBMCs can be successfully fused with polyethylenglicol (PEG) in order to generated viable osteoclast-like cells that exhibit tartrate-resistant acid phosphatase (TRAP) and bone resorptive activities...
March 25, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28332063/decreased-interaction-between-zo-1-and-occludin-is-involved-in-alteration-of-tight-junctions-in-transplanted-epiphora-submandibular-glands
#2
Chong Ding, Xin Cong, Xue-Ming Zhang, Sheng-Lin Li, Li-Ling Wu, Guang-Yan Yu
Tight junctions (TJs) in salivary epithelium play an important role in regulating saliva secretion. Autologous transplantation of submandibular glands (SMGs) is an effective method to treat severe dry eye syndrome. However, epiphora occurs in some patients 6 months after transplantation. We previously found that the acinar TJs are enlarged in rabbit SMGs after long-term transplantation, but the exact TJ components involved in the epiphora are still unknown. Here, we found that the mRNA and protein expression of ZO-1 and occludin were increased in the transplanted SMGs obtained from epiphora patients, while other TJs were unchanged...
March 22, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28317066/er%C3%AE-and-gnrh-co-localize-in-the-hypothalamic-neurons-of-the-south-american-plains-vizcacha-lagostomus-maximus-rodentia-caviomorpha
#3
Pablo Ignacio Felipe Inserra, Santiago Elías Charif, Noelia Paula Di Giorgio, Lucía Saucedo, Alejandro Raúl Schmidt, Nicolas Fraunhoffer, Julia Halperin, María Constanza Gariboldi, Noelia Paola Leopardo, Victoria Lux-Lantos, Candela Rocío Gonzalez, Alfredo Daniel Vitullo, Verónica Berta Dorfman
Gonadotropin-releasing hormone (GnRH) is the key regulator of the hypothalamic-pituitary-gonadal axis. Estradiol (E2) affects GnRH synthesis and delivery. Hypothalamic estrogen receptors (ER) modulate GnRH expression acting as transcription factors. The South American plains vizcacha, Lagostomus maximus, is able to ovulate up to 800 oocytes per reproductive cycle, and shows continuous folliculogenesis with pre-ovulatory follicle formation and an ovulatory event at mid-gestation. The aim of this work was to analyze the hypothalamic expression of ER in the vizcacha at different gestational time-points, and its relationship with GnRH expression, serum luteinizing hormone (LH) and E2...
March 19, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28299491/effects-of-androgen-receptor-mutation-on-testicular-histopathology-of-patient-having-complete-androgen-insensitivity
#4
Ihtisham Bukhari, Guangyuan Li, Liu Wang, Furhan Iqbal, Huan Zhang, Jiansheng Zhu, Hui Liu, Xiangdong Fang, Nasser M Al-Daghri, Howard J Cooke, Yuanwei Zhang, Xiaohua Jiang
Androgens are required for normal male sex differentiation and development of male secondary sexual characteristics. Mutations in AR gene are known to cause defects in male sexual differentiation. In current study, we enrolled a 46,XY phenotypically female patient bearing testes in inguinal canal. DNA sequencing of the AR gene detected a missense mutation C.1715A > G (p. Y572C) in exon 2 which is already known to cause complete androgen insensitivity syndrome (CAIS). We focused on the effects of this mutation on the testicular histopathology of the patient...
March 15, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28255782/neuroanatomical-distribution-of-galectin-3-in-the-adult-rat-brain
#5
Hong-Il Yoo, Eu-Gene Kim, Eun-Jin Lee, Sung-Young Hong, Chi-Sun Yoon, Min-Ju Hong, Sang-Jin Park, Ran-Sook Woo, Tai-Kyoung Baik, Dae-Yong Song
Galectin-3 is a member of the lectin subfamily that enables the specific binding of β-galactosides. It is expressed in a broad spectrum of species and organs, and is known to have various functions related to cell adhesion, signal transduction, and proinflammatory responses. Although, expression of galectin-3 in some activated neuroglia under neuroinflammation has been well documented in the central nervous system, little is known about the neuronal expression and distribution of galectin-3 in normal brain...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28247149/mir-155-promotes-cutaneous-wound-healing-through-enhanced-keratinocytes-migration-by-mmp-2
#6
Longlong Yang, Zhao Zheng, Qin Zhou, Xiaozhi Bai, Lei Fan, Chen Yang, Linlin Su, Dahai Hu
Inflammation, re-epithelization and tissue remodeling are three essential steps during wound healing. The re-epithelization process plays the most important role which mainly involves keratinocyte proliferation and migration. miR-155 has been reported to participate in cell migration and transformation, however, its function in skin wound healing is largely unknown. Here we hypothesize that overexpression of miR-155 at wound edges could accelerate wound healing mediated by enhanced keratinocyte migration. To test this hypothesis, direct local injection of miR-155 expression plasmid to wound edges was conducted to overexpress miR-155 in vivo...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28229263/exosomes-derived-from-human-amniotic-epithelial-cells-accelerate-wound-healing-and-inhibit-scar-formation
#7
Bin Zhao, Yijie Zhang, Shichao Han, Wei Zhang, Qin Zhou, Hao Guan, Jiaqi Liu, Jihong Shi, Linlin Su, Dahai Hu
Wound healing is a highly orchestrated physiological process consisting of a complex events, and scarless wound healing is highly desired for the development and application in clinical medicine. Recently, we have demonstrated that human amniotic epithelial cells (hAECs) promoted wound healing and inhibited scar formation through a paracrine mechanism. However, exosomes (Exo) are one of the most important paracrine factors. Whether exosomes derived from human amniotic epithelial cells (hAECs-Exo) have positive effects on scarless wound healing have not been reported yet...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28093664/cell-proliferation-and-differentiation-during-the-three-dimensional-reconstitution-of-eccrine-sweat-glands
#8
Xuexue Li, Haihong Li, Mingjun Zhang, Liyun Chen, Bingna Zhang
The aim of this study is to characterize the cell proliferation and proliferating cell types during three-dimensional reconstitution of eccrine sweat glands. Eccrine sweat gland cells suspended in Matrigel were injected subcutaneously into the inguinal regions of nude mice. At 1, 2, 4, 6, 8, 14, 21, 28, 35 and 42 days post-implantation, Matrigel plugs were immunostained for Ki67, to detect cycling cells, and the Ki67 labeling index at different time points was calculated. Three pairs of antibodies, Ki67/K7, Ki67/K14 and Ki67/α-SMA, were used to identify proliferating cell types in the plugs, on days 28, 35 and 42, by immunofluorescence double staining...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28078480/muscarinic-acetylcholine-receptor-mediated-tight-junction-opening-is-involved-in-epiphora-in-late-phase-of-submandibular-gland-transplantation
#9
Ning-Yan Yang, Chong Ding, Jing Li, Yan Zhang, Ruo-Lan Xiang, Li-Ling Wu, Guang-Yan Yu, Xin Cong
Submandibular gland (SMG) autotransplantation is an effective therapy for treating severe dry eye syndrome. However, epiphora occurs in more than 40% of patients 6 months after operation. We previously found that muscarinic acetylcholine receptor (mAChR) plays a crucial role in regulating SMG secretion partially through the modulation on tight junction (TJ)-based paracellular pathway. Therefore, the present study aimed to investigate the possible involvement of mAChR and TJ in a rabbit long-term model of SMG transplantation...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28000152/inactivation-of-bone-morphogenetic-protein-1-bmp1-and-tolloid-like-1-tll1-in-cells-expressing-type-i-collagen-leads-to-dental-and-periodontal-defects-in-mice
#10
Hua Zhang, Priyam Jani, Tian Liang, Yongbo Lu, Chunlin Qin
Bone morphogenetic protein 1 (BMP1) and tolloid-like 1 (TLL1) belong to the BMP1/tolloid-like proteinase family, which cleaves secretory proteins. The constitutive deletion of the Bmp1 or Tll1 genes causes perinatal or embryonic lethality in mice. In this study, we first studied the β-galactosidase activity in mice in which an IRES-lacZ-Neo cassette was inserted in the intron of either the Bmp1 or the Tll1 gene; the β-galactosidase activities were used to reflect the expression of endogenous Bmp1 and Tll1, respectively...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27981392/histological-evidence-that-metformin-reverses-the-adverse-effects-of-diabetes-on-orthodontic-tooth-movement-in-rats
#11
Jing Sun, Juan Du, Wei Feng, Boyao Lu, Hongrui Liu, Jie Guo, Norio Amizuka, Minqi Li
This study evaluated the effects of metformin on orthodontic tooth movement in a rat model of type 2 diabetes mellitus. Rats were fed a high-fat diet for 4 weeks to induce fat accumulation and insulin resistance, and then injected with a low dose of streptozotocin (35 mg/kg) intraperitoneally to induce type 2 diabetes. An orthodontic appliance was placed in normoglycemic, type 2 diabetes, and type 2 diabetes with metformin-administrated rats. After 14 days, type 2 diabetes rats exhibited greater orthodontic tooth movement and had a higher number of tartrate-resistant acid phosphatase-positive osteoclasts, stronger cathepsin K expression, and weaker alkaline phosphatase immunostaining than normoglycemic rats...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27913976/follistatin-like-protein-1-suppressed-pro-inflammatory-cytokines-expression-during-neuroinflammation-induced-by-lipopolysaccharide
#12
Kai-Yuan Cheng, Yi Liu, Ying-Guang Han, Jing-Kun Li, Jia-Lin Jia, Bin Chen, Zhi-Xiao Yao, Lin Nie, Lei Cheng
Follistain-like protein 1 (FSTL1), has been recently demonstrated to be involved in the embryo development of nervous system and glioblastoma. However, the role of FSTL1 in neuroinflammation remains unexplored. In this study, the expression of FSTL1 in astrocytes was verified and its role was studied in neuroinflammation induced by in vivo intracerebroventricular (ICV) injection of lipopolysaccharide (LPS) or LPS treatment to astrocytes in vitro. FSTL1 was significantly induced after ICV LPS injection or LPS treatment...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27885497/erratum-to-a-zebrafish-mosaic-assay-to-study-mammalian-stem-cells-in-real-time-in-vivo
#13
Chun Xiao, Meilin Qian, Chaoran Yin, Yonggang Zhang, Huozhen Hu, Shaohua Yao
No abstract text is available yet for this article.
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27882438/histochemical-examination-of-systemic-administration-of-eldecalcitol-combined-with-guided-bone-regeneration-for-bone-defect-restoration-in-rats
#14
Xiuchun Han, Juan Du, Di Liu, Hongrui Liu, Norio Amizuka, Minqi Li
The aim of this experiment was to elucidate the histological alterations after systemic administration of eldecalcitol (ELD) combined with guided bone regeneration during the restoration of bone defect healing in rats. The femurs of 8-week-old Wister rats were used to generate bone defect models. The defect was covered with a collagen membrane, and ELD group was administrated with eldecalcitol (50 ng/kg body weight) intragastrically once every other day. Femora were harvested at 1, 2, 4 and 8 weeks post-surgery...
February 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27866302/expression-analysis-of-dickkopf-related-protein-3-dkk3-suggests-its-pleiotropic-roles-for-a-secretory-glycoprotein-in-adult-mouse
#15
Junji Inoue, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Hiromi Kumon, Hideyo Ohuchi
Dickkopf-related protein 3 (Dkk3) is the third member of the Dkk gene family and identical to the gene, whose expression was reduced in immortalized cells. Therefore, its another name is reduced expression in immortalized cells. Since the intratumoral introduction of Dkk3 inhibits tumor growth in mouse models of cancers, Dkk3 is likely a tumor suppressor gene. However, the functions of Dkk3 in vivo remain unclear. As the first step to decipher the physiological roles of this gene, we examined the expression pattern of Dkk3 in various tissues from adult mice...
February 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27803997/evaluating-the-effect-of-silver-nanoparticles-on-testes-of-adult-albino-rats-histological-immunohistochemical-and-biochemical-study
#16
Samah M Ahmed, Shaimaa A Abdelrahman, Sally M Shalaby
Silver nanoparticles (AgNPs) are widely used in medicine, however, they have toxic impacts on different organs. AgNPs distribution to the testes was reported, so, we aimed to study the effect of intraperitoneal injection of AgNPs, at different concentrations and different time durations, on adult rat testes. Sixty healthy adult male Wistar albino rats were divided into three groups; control group (Group I) and two experimental groups (Groups II & III), each of which were subdivided into two subgroups. Rats in group II were exposed for 7 days to low and high doses of AgNPs, respectively...
February 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27803996/crim1-is-necessary-for-coronary-vascular-endothelial-cell-development-and-homeostasis
#17
Swati Iyer, Yash Chhabra, Tracey J Harvey, Richard Wang, Han Sheng Chiu, A G Smith, Walter G Thomas, David J Pennisi, Michael Piper
Endothelial cells form a critical component of the coronary vasculature, yet the factors regulating their development remain poorly defined. Here we reveal a novel role for the transmembrane protein CRIM1 in mediating cardiac endothelial cell development. In the absence of Crim1 in vivo, the coronary vasculature is malformed, the number of endothelial cells reduced, and the canonical BMP pathway dysregulated. Moreover, we reveal that CRIM1 can bind IGFs, and regulate IGF signalling within endothelial cells...
February 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27787633/foxa1-gene-and-protein-in-developing-rat-eccrine-sweat-glands
#18
Haihong Li, Liyun Chen, Mingjun Zhang, Bingna Zhang
To investigate the development of eccrine sweat glands and the expression of Foxa1 genes and proteins in the course of development, the footpads from E15.5 to E21.5, P1-P12, P14, P21, P28 and P56 rats were subjected to immunofluorescence staining of FoxA1 and double immunofluorescence staining of K14/α-SMA, FoxA1/K7 and FoxA1/α-SMA, and were processed for Foxa1 gene detection by RT-qPCR. The results showed that rat eccrine sweat gland germs was first observed emerging from the basal layer of epidermis at E19...
February 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27722791/a-modified-protocol-for-the-detection-of-three-different-mrnas-with-a-new-generation-in-situ-hybridization-chain-reaction-on-frozen-sections
#19
Qian-Qian Sui, Jiao Zhu, Xiangnan Li, Gillian E Knight, Cheng He, Geoffrey Burnstock, Hongbin Yuan, Zhenghua Xiang
A new multiple fluorescence in situ hybridization method based on hybridization chain reaction was recently reported, enabling simultaneous mapping of multiple target mRNAs within intact zebrafish and mouse embryos. With this approach, DNA probes complementary to target mRNAs trigger chain reactions in which metastable fluorophore-labeled DNA hairpins self-assemble into fluorescent amplification polymers. The formation of the specific polymers enhances greatly the sensitivity of multiple fluorescence in situ hybridization...
December 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27714564/heat-shock-protein-27-hsp27-regulates-stat3-and-eif4g-levels-in-first-trimester-human-placenta
#20
Gali Epstein Shochet, Oded Komemi, Dana Sadeh-Mestechkin, Meir Pomeranz, Ami Fishman, Liat Drucker, Michael Lishner, Shelly Tartakover Matalon
During placental implantation, cytotrophoblast cells differentiate to extravillous trophoblast (EVT) cells that invade from the placenta into the maternal uterine blood vessels. The heat shock protein-27 (HSP27), the signal transducer and activator of transcription-3 (STAT3) and the eukaryotic translation initiation factor 4E (EIF4E) are involved in regulating EVT cell differentiation/migration. EIF4E and EIF4G compose the translation initiation complex, which is a major control point in protein translation...
December 2016: Journal of Molecular Histology
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