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Journal of Molecular Histology

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https://www.readbyqxmd.com/read/28687932/spatial-and-temporal-expression-of-sox9-during-murine-incisor-development
#1
Zhi-Cheng Yang, Dan Li, Shuang Feng, Xiao-Dong Sui, Zhi Chen, Li Zhang
Mouse incisors are capable of continuously growing due to the renewal of dental epithelium stem cells and mesenchymal stem cells residing at the proximal ends. The transcription factor Sox9 plays important roles in maintaining the stem cells of hair follicles, retinal progenitor cells and neural crest stem cells. Whether Sox9 is involved during mouse incisor development is not reported yet. In this study, we examined the expression pattern of Sox9 during mouse incisor development by in situ hybridization and immunohistochemistry...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28647773/cthrc1-promotes-osteogenic-differentiation-of-periodontal-ligament-stem-cells-by-regulating-taz
#2
Chengze Wang, Weiting Gu, Baiyu Sun, Yunpeng Zhang, Yawen Ji, Xin Xu, Yong Wen
Collagen triple helix repeat containing 1 (CTHRC1) is associated with bone metabolism. Alveolar bone has an ability to rapidly remodel itself to adapt its biomechanical environment and function. However, whether CTHRC1 is expressed in alveolar bone tissue and the role of CTHRC1 in alveolar bone remodeling remain unclear. We used orthodontic tooth movement (OTM) rat model to study the effects of CHTRC1 in alveolar bone remodeling in vivo. We found that CTHRC1 was expressed in normal physiological condition of osteocytes, bone matrix, and periodontal ligament cells in rat...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28643114/synergistic-cardioprotective-effects-of-raav9-cyclina2-combined-with-fibrin-glue-in-rats-after-myocardial-infarction
#3
Wen Cao, Ya-Fei Chang, Ai-Chao Zhao, Bang-Dang Chen, Fen Liu, Yi-Tong Ma, Xiang Ma
The present study aimed to investigate the protective effects of rAAV9-CyclinA2 combined with fibrin glue (FG) in vivo in rats after myocardial infarction (MI). Ninety male Sprague-Dawley rats were randomized into 6 groups (15 in each group): sham, MI, rAAV9-green fluorescent protein (GFP) + MI, rAAV9-CyclinA2 + MI, FG + MI, and rAAV9-CyclinA2 + FG + MI. Packed virus (5 × 10(11)vg/ml) in 150 µl of normal saline or FG was injected into the infarcted myocardium at five locations in rAAV9-GFP + MI, rAAV9-CyclinA2 + MI, and rAAV9-CyclinA2 + FG + MI groups...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28597201/methane-alleviates-carbon-tetrachloride-induced-liver-injury-in-mice-anti-inflammatory-action-demonstrated-by-increased-pi3k-akt-gsk-3%C3%AE-mediated-il-10-expression
#4
Ying Yao, Liping Wang, Peipei Jin, Na Li, Yan Meng, Changli Wang, Mengda Xu, Yan Zhang, Jinjun Bian, Xiaoming Deng
The inflammatory response plays an important role in carbon tetrachloride (CCl4)-induced acute liver injury and methane has been shown to exert beneficial effects on inflammation-associated diseases. Thus, we investigated the potential protective effects of methane-rich saline (MS) on CCl4-induced acute liver injury and explored the underlying mechanism. A CCl4-induced acute liver injury model was established by injection of CCl4 (0.6 ml/kg, ip) in mice followed by treatment with MS (16 ml/kg, ip), 24 h later...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28550642/post-heroin-dose-tissue-distribution-of-6-monoacetylmorphine-6-mam-with-maldi-imaging
#5
Belin G Teklezgi, Annapurna Pamreddy, Sooraj Baijnath, Nirmala D Gopal, Tricia Naicker, Hendrik G Kruger, Thavendran Govender
Heroin is an illicit opioid drug which is commonly abused and leads to dependence and addiction. Heroin is considered a pro-drug and is rapidly converted to its major active metabolite 6-monoacetylmorphine (6-MAM) which mediates euphoria and reward through the stimulation of opioid receptors in the brain. The aim of this study was to investigate the distribution and localization of 6-MAM in the healthy Sprague Dawley rat brain following intraperitoneal (i.p) administration of heroin (10 mg/kg), using matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI), in combination with quantification via liquid chromatography mass spectrometry (LC-MS/MS)...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28547585/aio-casilio-a-robust-crispr-cas9-pumilio-system-for-chromosome-labeling
#6
Shuxian Zhang, Zhan Song
The biological function of chromatin bases on its spatial organization and dynamic activities in different situations. Labeling and tracing of genomic sequences has been a huge challenge in studying the spatial dynamics of chromatin. We reported the development of 'all-in-one Casilio system (Aio-Casilio)', a new system that enables the labeling of endogenous genomic loci. The Aio-Casilio system consists of the dCas9 protein, mClover fused with the Pumilio and FBF proteins RNA-binding domain (PUF domain) and an U6-sgRNA appended with multiple PUF-binding site(s)...
August 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28455549/involution-processes-of-follicular-atresia-and-post-ovulatory-complex-in-a-characid-fish-ovary-a-study-of-apoptosis-and-autophagy-pathways
#7
Mônica Cassel, Marília de Paiva Camargo, Lázaro Wender Oliveira de Jesus, Maria Inês Borella
Recent studies seem to indicate that apoptosis and autophagy can act cooperatively in fish ovaries in order to achieve more effective ovarian regression after spawning. Considering the importance of tissue remodeling in ovarian functioning, we sought to morphologically characterize the involution processes of follicular atresia and post-ovulatory complexes using Astyanax altiparanae as an experimental model, and to determine the location of proteins involved in apoptosis and autophagy throughout this process...
April 28, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28451773/correlation-between-three-dimensional-power-doppler-and-morphometric-measurement-of-endometrial-vascularity-at-the-time-of-embryo-implantation-in-women-with-unexplained-recurrent-miscarriage
#8
Xiaoyan Chen, Sotirios H Saravelos, Yingyu Liu, Jin Huang, Chi Chiu Wang, Tin Chiu Li
Power Doppler in combination with three-dimensional (3D-PD) ultrasonography has been used as a noninvasive tool to evaluate the vascularity. However, it is unclear whether 3D-PD can accurately reflect endometrial vascularization and replace the invasive endometrial biopsy. This study aims to investigate the correlation between 3D-PD and micro vessel morphometric measurement of endometrial vascularity. Twenty-five women with unexplained recurrent miscarriage were recruited for 3D-PD and endometrial biopsy on precisely day LH + 7...
April 27, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28409326/effect-of-corticision-on-orthodontic-tooth-movement-in-a-rat-model-as-assessed-by-rna-sequencing
#9
Qihui Gu, Shuyu Guo, Dongyue Wang, Tingting Zhou, Lin Wang, Zhendong Wang, Junqing Ma
Corticision is a common technique to accelerate orthodontic tooth movement; however, not much is known about the underlying mechanisms. In this study, we investigated the mechanism of alveolar tissue remodeling after corticision in a rat model of tooth movement (TM) by analyzing the differential transcriptome. A total of 36 male rats were equally divided into TM and TM with corticision (TM+C) groups. Alveolar bone response was examined using micro-computed tomography (micro-CT). Osteoclasts and osteoblasts were quantified on tartrate-resistant acid phosphatase (TRAP) and Goldner's trichrome staining...
April 13, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28393293/gata4-regulates-osteoblastic-differentiation-and-bone-remodeling-via-p38-mediated-signaling
#10
Tingting Zhou, Shuyu Guo, Yuxin Zhang, Yajuan Weng, Lin Wang, Junqing Ma
Osteoblasts play a major role in bone remodeling and are regulated by transcription factors. GATA4, a zinc finger transcription factor from the GATA family, has an unclear role in osteoblast differentiation. In this study, the role of GATA4 in osteoblast differentiation was studied both in vitro and in vivo by GATA4 knockdown. GATA4 expression increased during osteoblast differentiation. GATA4 knockdown in osteoblast precursor cells reduced alkaline phosphatase activity and decreased the formation of calcified nodule in an osteogenic-induced cell culture system...
April 9, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28374152/uric-acid-activates-nrlp3-inflammasome-in-an-in-vivo-model-of-epithelial-to-mesenchymal-transition-in-the-kidney
#11
César Andrés Romero, Aline Remor, Alexandra Latini, Ana Lucía De Paul, Alicia Inés Torres, Jorge Humberto Mukdsi
Uric acid (UA) has been associated with renal fibrosis and progression of chronic kidney disease. However, the underlying mechanisms of this process have still not been identified. Here, we studied the role of the innate imunity receptor NLRP3/ASC in UA induced epithelial-mesenchymal transition (EMT) in kidney. Wistar rats were fed with oxonic acid 2% and UA 2% (OXA + U), OXA + U plus allopurinol (ALL) or regular chow (C) for 7 weeks. We analyzed the presence of EMT markers, the expression of NLRP3, ASC, Caspase-1 and Smad 2/3 molecules and the mitochondrial morphological and functional characteristics...
April 3, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28255782/neuroanatomical-distribution-of-galectin-3-in-the-adult-rat-brain
#12
Hong-Il Yoo, Eu-Gene Kim, Eun-Jin Lee, Sung-Young Hong, Chi-Sun Yoon, Min-Ju Hong, Sang-Jin Park, Ran-Sook Woo, Tai-Kyoung Baik, Dae-Yong Song
Galectin-3 is a member of the lectin subfamily that enables the specific binding of β-galactosides. It is expressed in a broad spectrum of species and organs, and is known to have various functions related to cell adhesion, signal transduction, and proinflammatory responses. Although, expression of galectin-3 in some activated neuroglia under neuroinflammation has been well documented in the central nervous system, little is known about the neuronal expression and distribution of galectin-3 in normal brain...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28247149/mir-155-promotes-cutaneous-wound-healing-through-enhanced-keratinocytes-migration-by-mmp-2
#13
Longlong Yang, Zhao Zheng, Qin Zhou, Xiaozhi Bai, Lei Fan, Chen Yang, Linlin Su, Dahai Hu
Inflammation, re-epithelization and tissue remodeling are three essential steps during wound healing. The re-epithelization process plays the most important role which mainly involves keratinocyte proliferation and migration. miR-155 has been reported to participate in cell migration and transformation, however, its function in skin wound healing is largely unknown. Here we hypothesize that overexpression of miR-155 at wound edges could accelerate wound healing mediated by enhanced keratinocyte migration. To test this hypothesis, direct local injection of miR-155 expression plasmid to wound edges was conducted to overexpress miR-155 in vivo...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28229263/exosomes-derived-from-human-amniotic-epithelial-cells-accelerate-wound-healing-and-inhibit-scar-formation
#14
Bin Zhao, Yijie Zhang, Shichao Han, Wei Zhang, Qin Zhou, Hao Guan, Jiaqi Liu, Jihong Shi, Linlin Su, Dahai Hu
Wound healing is a highly orchestrated physiological process consisting of a complex events, and scarless wound healing is highly desired for the development and application in clinical medicine. Recently, we have demonstrated that human amniotic epithelial cells (hAECs) promoted wound healing and inhibited scar formation through a paracrine mechanism. However, exosomes (Exo) are one of the most important paracrine factors. Whether exosomes derived from human amniotic epithelial cells (hAECs-Exo) have positive effects on scarless wound healing have not been reported yet...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28093664/cell-proliferation-and-differentiation-during-the-three-dimensional-reconstitution-of-eccrine-sweat-glands
#15
Xuexue Li, Haihong Li, Mingjun Zhang, Liyun Chen, Bingna Zhang
The aim of this study is to characterize the cell proliferation and proliferating cell types during three-dimensional reconstitution of eccrine sweat glands. Eccrine sweat gland cells suspended in Matrigel were injected subcutaneously into the inguinal regions of nude mice. At 1, 2, 4, 6, 8, 14, 21, 28, 35 and 42 days post-implantation, Matrigel plugs were immunostained for Ki67, to detect cycling cells, and the Ki67 labeling index at different time points was calculated. Three pairs of antibodies, Ki67/K7, Ki67/K14 and Ki67/α-SMA, were used to identify proliferating cell types in the plugs, on days 28, 35 and 42, by immunofluorescence double staining...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28078480/muscarinic-acetylcholine-receptor-mediated-tight-junction-opening-is-involved-in-epiphora-in-late-phase-of-submandibular-gland-transplantation
#16
Ning-Yan Yang, Chong Ding, Jing Li, Yan Zhang, Ruo-Lan Xiang, Li-Ling Wu, Guang-Yan Yu, Xin Cong
Submandibular gland (SMG) autotransplantation is an effective therapy for treating severe dry eye syndrome. However, epiphora occurs in more than 40% of patients 6 months after operation. We previously found that muscarinic acetylcholine receptor (mAChR) plays a crucial role in regulating SMG secretion partially through the modulation on tight junction (TJ)-based paracellular pathway. Therefore, the present study aimed to investigate the possible involvement of mAChR and TJ in a rabbit long-term model of SMG transplantation...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28000152/inactivation-of-bone-morphogenetic-protein-1-bmp1-and-tolloid-like-1-tll1-in-cells-expressing-type-i-collagen-leads-to-dental-and-periodontal-defects-in-mice
#17
Hua Zhang, Priyam Jani, Tian Liang, Yongbo Lu, Chunlin Qin
Bone morphogenetic protein 1 (BMP1) and tolloid-like 1 (TLL1) belong to the BMP1/tolloid-like proteinase family, which cleaves secretory proteins. The constitutive deletion of the Bmp1 or Tll1 genes causes perinatal or embryonic lethality in mice. In this study, we first studied the β-galactosidase activity in mice in which an IRES-lacZ-Neo cassette was inserted in the intron of either the Bmp1 or the Tll1 gene; the β-galactosidase activities were used to reflect the expression of endogenous Bmp1 and Tll1, respectively...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27981392/histological-evidence-that-metformin-reverses-the-adverse-effects-of-diabetes-on-orthodontic-tooth-movement-in-rats
#18
Jing Sun, Juan Du, Wei Feng, Boyao Lu, Hongrui Liu, Jie Guo, Norio Amizuka, Minqi Li
This study evaluated the effects of metformin on orthodontic tooth movement in a rat model of type 2 diabetes mellitus. Rats were fed a high-fat diet for 4 weeks to induce fat accumulation and insulin resistance, and then injected with a low dose of streptozotocin (35 mg/kg) intraperitoneally to induce type 2 diabetes. An orthodontic appliance was placed in normoglycemic, type 2 diabetes, and type 2 diabetes with metformin-administrated rats. After 14 days, type 2 diabetes rats exhibited greater orthodontic tooth movement and had a higher number of tartrate-resistant acid phosphatase-positive osteoclasts, stronger cathepsin K expression, and weaker alkaline phosphatase immunostaining than normoglycemic rats...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27913976/follistatin-like-protein-1-suppressed-pro-inflammatory-cytokines-expression-during-neuroinflammation-induced-by-lipopolysaccharide
#19
Kai-Yuan Cheng, Yi Liu, Ying-Guang Han, Jing-Kun Li, Jia-Lin Jia, Bin Chen, Zhi-Xiao Yao, Lin Nie, Lei Cheng
Follistain-like protein 1 (FSTL1), has been recently demonstrated to be involved in the embryo development of nervous system and glioblastoma. However, the role of FSTL1 in neuroinflammation remains unexplored. In this study, the expression of FSTL1 in astrocytes was verified and its role was studied in neuroinflammation induced by in vivo intracerebroventricular (ICV) injection of lipopolysaccharide (LPS) or LPS treatment to astrocytes in vitro. FSTL1 was significantly induced after ICV LPS injection or LPS treatment...
April 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27885497/erratum-to-a-zebrafish-mosaic-assay-to-study-mammalian-stem-cells-in-real-time-in-vivo
#20
Chun Xiao, Meilin Qian, Chaoran Yin, Yonggang Zhang, Huozhen Hu, Shaohua Yao
No abstract text is available yet for this article.
April 2017: Journal of Molecular Histology
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