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Journal of Translational Medicine

Weiling He, Di Xu, Zhuo Wang, Hui Wu, Xianhong Xiang, Bing Tang, Wenting Jiang, Yongmei Cui, Han Wang, Neng Jiang, Yu Sun, Yangshan Chen, Shuhua Li, Minzhi Hou, Yang Zhang, Liantang Wang, Zun-Fu Ke
BACKGROUND: Circulating tumor cells (CTC) shows great prospect to realize precision medicine in cancer patients. METHODS: We developed the NanoVelcro Chip integrating three functional mechanisms. NanoVelcro CTC capture efficiency was tested in stage III or IV lung adenocarcinoma. Further, ALK-rearrangement status was examined through fluorescent in situ hybridization in CTCs enriched by NanoVelcro. RESULTS: NanoVelcro system showed higher CTC-capture efficiency than CellSearch in stage III or IV lung adenocarcinoma...
January 18, 2019: Journal of Translational Medicine
Peter Kim Moon, Stephanie Tran, Paras Singh Minhas
Botticelli et al. proposed the activity of indoleamine-2,3-dioxygenase 1 (IDO) as a potential mechanism and predictive marker for primary resistance against anti-PD-1 treatment in the context of non-small cell lung cancer. However, there are a few points for the authors to address in order to strengthen their claims. First, there are many enzymes that modulate the kynurenine to tryptophan ratio, thereby calling into question their use of the ratio as a proxy for IDO activity. Second, the authors could compare IDO to other proposed markers in the literature, providing a better understanding of its predictive value...
January 18, 2019: Journal of Translational Medicine
Ke Zhang, Yong-Qiang Hua, Dan Wang, Lian-Yu Chen, Cai-Jun Wu, Zhen Chen, Lu-Ming Liu, Hao Chen
BACKGROUND: Systemic inflammation and immune dysfunction have been proved to be associated with cancer progression and metastasis in various malignancies. The aim of this retrospective study was to evaluate the prognostic significance of pre-treatment systemic immune-inflammation index (SII) in patients with advanced pancreatic cancer. METHODS: In total, 419 patients diagnosed with advanced pancreatic cancer, between January 2011 and December 2015, were retrospectively enrolled...
January 18, 2019: Journal of Translational Medicine
Hideki Furuya, Owen T M Chan, Ian Pagano, Chengjun Zhu, Nari Kim, Rafael Peres, Kanani Hokutan, Sarah Alter, Peter Rhode, Charles J Rosser
BACKGROUND: We set out to determine if the administration of subcutaneous (SQ) ALT-803 was non-inferior to standard intravesical BCG treatment in a carcinogen induced mouse (C57BL/6J) bladder cancer model. METHODS: Using this well-established carcinogen induced mouse model, we studied the effects of various dosing schemas of ALT-803 (SQ alone, SQ with intravesical BCG, intravesical alone, intravesical with intravesical BCG) compared to intravesical BCG alone (positive control) and PBS (negative control)...
January 17, 2019: Journal of Translational Medicine
Yi Liu, Yizuo Song, Miaomiao Ye, Xiaoli Hu, Z Peter Wang, Xueqiong Zhu
Accumulated evidence has demonstrated that WNT1 inducible signaling pathway protein (WISP) genes, which belong to members of the CCN growth factor family, play a pivotal role in tumorigenesis and progression of a broad spectrum of human cancers. Mounting studies have identified that WISP proteins (WISP1-3) exert different biological functions in various human malignancies. Emerging evidence indicates that WISP proteins are critically involved in cell proliferation, apoptosis, invasion and metastasis in cancers...
January 16, 2019: Journal of Translational Medicine
Jingyu Liu, Jing Li, Hui Wang, Yikai Wang, Qiongzhi He, Xuefeng Xia, Zhe-Yu Hu, Quchang Ouyang
BACKGROUND: Among breast cancer (BC) patients, near 40% are post-menopause, and 70%-80% are hormone receptor (HR)-positive. About 30%-40% BC patients who are diagnosed as invasive carcinoma HR-positive BC would eventually develop metastatic breast cancers. In 2016, FALCON trial proves Fulvestrant as an effective first-line endocrine therapy for post-menopause HR-positive advanced BC (ABC) patients. But even after FALCON published, Fulvestrant is rarely used as first-line in real world ABC patients in China...
January 15, 2019: Journal of Translational Medicine
Thomas Kerforne, Frédéric Favreau, Tackwa Khalifeh, Souleymane Maiga, Geraldine Allain, Antoine Thierry, Manuel Dierick, Edouard Baulier, Clara Steichen, Thierry Hauet
BACKGROUND: Renal transplantation is increasingly associated with the presence of comorbidity factors such as dyslipidemia which could influence the graft outcome. We hypothesized that hypercholesterolemia could affect vascular repair processes and promote post-transplant renal vascular remodeling through the over-expression of the anti-angiogenic thrombospondin-1 interacting with vascular endothelial growth factor-A levels. METHODS: We tested this hypothesis in vitro, in vivo and in a human cohort using (1) endothelial cells; (2) kidney auto-transplanted pig subjected (n = 5) or not (n = 6) to a diet enriched in cholesterol and (3) a renal transplanted patient cohort (16 patients)...
January 14, 2019: Journal of Translational Medicine
Liu Miao, Rui-Xing Yin, Shang-Ling Pan, Shuo Yang, De-Zhai Yang, Wei-Xiong Lin
BACKGROUND: The present study attempted to identify potential key genes and miRNAs of dyslipidemia in obese, and to investigate the possible mechanisms associated with them. METHODS: The microarray data of GSE66676 were downloaded, including 67 obese samples from the Gene Expression Omnibus (GEO) database. The weighted gene co-expression network (WGCNA) analysis was performed using WGCNA package and grey60 module was considered as the highest correlation. Gene Ontology annotation and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses for this module were performed by clusterProfiler and DOSE package...
January 14, 2019: Journal of Translational Medicine
Flavia De Carlo, Litty Thomas, Bell Brooke, Elliot T Varney, Rounak Nande, Olivia Boskovic, Gailen D Marshall, Pier Paolo Claudio, Candace M Howard
BACKGROUND: Gene transfer to malignant sites using human adenoviruses (hAds) has been limited because of their immunogenic nature and host specificity. Murine cells often lack some of the receptors needed for hAds attachment, thus murine cells are generally non-permissive for human adenoviral infection and replication, which limits translational studies. METHODS: We have developed a gene transfer method that uses a combination of lipid-encapsulated perfluorocarbon microbubbles and ultrasound to protect and deliver hAds to a target tissue, bypassing the requirement of specific receptors...
January 11, 2019: Journal of Translational Medicine
Shutong Liu, David F Stroncek, Yingdong Zhao, Victoria Chen, Rongye Shi, Jinguo Chen, Jiaqiang Ren, Hui Liu, Hee Joon Bae, Steven L Highfill, Ping Jin
BACKGROUND: Bone marrow stromal cells (BMSCs) are a heterogeneous population that participates in wound healing, immune modulation and tissue regeneration. Next generation sequencing was used to analyze transcripts from single BMSCs in order to better characterize BMSC subpopulations. METHODS: Cryopreserved passage 2 BMSCs from one healthy subject were cultured through passage 10. The transcriptomes of bulk BMSCs from designated passages were analyzed with microarrays and RNA sequencing (RNA-Seq)...
January 11, 2019: Journal of Translational Medicine
Dominic Henn, Masood Abu-Halima, Dominik Wermke, Florian Falkner, Benjamin Thomas, Christoph Köpple, Nicole Ludwig, Matthias Schulte, Marc A Brockmann, Yoo-Jin Kim, Justin M Sacks, Ulrich Kneser, Andreas Keller, Eckart Meese, Volker J Schmidt
BACKGROUND: Vascular shear stress promotes endothelial cell sprouting in vitro. The impact of hemodynamic forces on microRNA (miRNA) and gene expression within growing vascular networks in vivo, however, remain poorly investigated. Arteriovenous (AV) shunts are an established model for induction of neoangiogenesis in vivo and can serve as a tool for analysis of hemodynamic effects on miRNA and gene expression profiles over time. METHODS: AV shunts were microsurgically created in rats and explanted on postoperative days 5, 10 and 15...
January 11, 2019: Journal of Translational Medicine
Tao Wang, Nianguo Dong, Huimin Yan, Sze Yue Wong, Wen Zhao, Kang Xu, Dong Wang, Song Li, Xuefeng Qiu
BACKGROUND: Vascular grafts are widely used as a treatment in coronary artery bypass surgery, hemodialysis, peripheral arterial bypass and congenital heart disease. Various types of synthetic and natural materials were experimented to produce tissue engineering vascular grafts. In this study, we investigated in vivo tissue engineering technology in miniature pigs to prepare decellularized autologous extracellular matrix-based grafts that could be used as vascular grafts for small-diameter vascular bypass surgery...
January 11, 2019: Journal of Translational Medicine
Immacolata Maida, Paola Zanna, Stefania Guida, Anna Ferretta, Tiziana Cocco, Luigi Leonardo Palese, Paola Londei, Dario Benelli, Amalia Azzariti, Stefania Tommasi, Michele Guida, Giovanni Pellacani, Gabriella Guida
BACKGROUND: Melanoma cells develop adaptive responses in order to cope with particular conditions of tumor microenvironment, characterized by stress conditions and deregulated proliferation. Recently, the interplay between the stress response and the gene expression programs leading to metastatic spread has been reported. METHODS: We evaluated levels and localization of eIF2α/peIF2α in V600BRAF and wtBRAF metastatic melanoma cell lines by means of western blot and confocal microscopy analyses...
January 11, 2019: Journal of Translational Medicine
Zhixiong Zhong, Heming Wu, Qifeng Zhang, Wei Zhong, Pingsen Zhao
BACKGROUND: T cells are key regulators of immunity and one of the cells recruited in atherosclerosis and participated in various stages of the development of atherosclerosis. Characterizing T-cell receptor (TCR) repertoires is a priority of great scientific interest and potential clinical utility for the early diagnosis, risk stratification and prognostic evaluation of acute myocardial infarction (AMI). METHODS: The TCR repertoires in 21 subjects including 7 patients with non-ST-segment elevation myocardial infarction (NSTEMI), 6 patients with ST-segment elevation myocardial infarction (STEMI) and 8 subjects with normal coronary artery (NCA) as control were characterized by using high-throughput sequencing...
January 11, 2019: Journal of Translational Medicine
Hong-Ki Min, JeongWon Choi, Seon-Yeong Lee, Hyeon-Beom Seo, KyungAh Jung, Hyun Sik Na, Jun-Geol Ryu, Seung-Ki Kwok, Mi-La Cho, Sung-Hwan Park
BACKGROUND: Spondyloarthritis (SpA) is chronic inflammatory arthritis, and interleukin (IL)-17 is crucial in SpA pathogenesis. Type 17 helper T (Th17) cells are one of major IL-17-secreting cells. Signal transducer and activator of transcription (STAT)-3 signaling induces Th17 differentiation. This study investigated the effects of protein inhibitor of activated STAT3 (PIAS3) on SpA pathogenesis. Curdlan was injected into SKG ZAP-70W163C mice for SpA induction. METHODS: The PIAS3 or Mock vector was inserted into mice for 10 weeks...
January 10, 2019: Journal of Translational Medicine
Raúl F Pérez, Pablo Santamarina, Juan Ramón Tejedor, Rocío G Urdinguio, Julio Álvarez-Pitti, Pau Redon, Agustín F Fernández, Mario F Fraga, Empar Lurbe
BACKGROUND: Early life is a period of drastic epigenetic remodeling in which the epigenome is especially sensitive to extrinsic and intrinsic influence. However, the epigenome-wide dynamics of the DNA methylation changes that occur during this period have not been sufficiently characterized in longitudinal studies. METHODS: To this end, we studied the DNA methylation status of more than 750,000 CpG sites using Illumina MethylationEPIC arrays on 33 paired blood samples from 11 subjects at birth and at 5 and 10 years of age, then characterized the chromatin context associated with these loci by integrating our data with histone, chromatin-state and enhancer-element external datasets, and, finally, validated our results through bisulfite pyrosequencing in two independent longitudinal cohorts of 18 additional subjects...
January 9, 2019: Journal of Translational Medicine
Wai Hoong Chang, Donall Forde, Alvina G Lai
BACKGROUND: Despite much progress in cancer research, its incidence and mortality continue to rise. A robust biomarker that would predict tumor behavior is highly desirable and could improve patient treatment and prognosis. METHODS: In a retrospective bioinformatics analysis involving patients with liver cancer (n = 839), we developed a prognostic signature consisting of 45 genes associated with tumor-infiltrating lymphocytes and cellular responses to hypoxia...
January 9, 2019: Journal of Translational Medicine
Koji Takada, Shinichiro Kashiwagi, Yuka Asano, Wataru Goto, Katsuyuki Takahashi, Hisakazu Fujita, Tsutomu Takashima, Shuhei Tomita, Kosei Hirakawa, Masaichi Ohira
BACKGROUND: The immune tumor microenvironment (iTME) is thought to affect the response to chemotherapy, and tumor-infiltrating lymphocytes (TILs) are often used as an indicator to evaluate the iTME. Smoking is involved in carcinogenesis, the relationship between smoking and the iTME of lung cancer has been reported. We hypothesized that smoking would affect the iTME of breast cancer and aimed to examine this relationship based on the amount of pre-diagnosis smoking and the subsequent effects on treatment response and prognosis...
January 7, 2019: Journal of Translational Medicine
Valentina Masola, Amedeo Carraro, Simona Granata, Lorenzo Signorini, Gloria Bellin, Paola Violi, Antonio Lupo, Umberto Tedeschi, Maurizio Onisto, Giovanni Gambaro, Gianluigi Zaza
BACKGROUND: The epithelial to mesenchymal transition (EMT) is a multi-factorial biological mechanism involved in renal and hepatic fibrosis and the IL-1 beta has been assumed as a mediator of this process although data are not exhaustive. Therefore, the aim of our study was to evaluate the role of this cytokine in the EMT of renal proximal tubular epithelial cells (HK-2) and stellate cells (LX-2) and the protective/anti-fibrotic effect of its inhibition by Canakinumab (a specific human monoclonal antibody targeted against IL-1beta)...
January 7, 2019: Journal of Translational Medicine
Zhaohui Cui, Zhixiao Wang, Xiaoyun Liu, Yun Cai, Xinyu Xu, Tao Yang
BACKGROUND: A clinical diagnosis model include thyroid functions, thyroid antibodies and radioactive iodine uptake (RAIU) of patients with hyperthyroidism were established and as new evaluation indicators for the differentiation of the Graves' disease (GD) and Hashimoto's thyroiditis (HT). METHODS: Clinical data of patients with newly diagnosed hyperthyroidism including gender, age, thyroid function, thyroid antibodies (FT3, FT4, TSH, TPOAb, TGAb, TRAb), RAIU (2 h, 6 h, 24 h) were collected...
January 7, 2019: Journal of Translational Medicine
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