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Cancer Genomics & Proteomics

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https://www.readbyqxmd.com/read/28031240/combination-of-a-stat3-inhibitor-and-an-mtor-inhibitor-against-a-temozolomide-resistant-glioblastoma-cell-line
#1
Haruo Miyata, Tadashi Ashizawa, Akira Iizuka, Ryota Kondou, Chizu Nonomura, Takashi Sugino, Kenichi Urakami, Akira Asai, Nakamasa Hayashi, Koichi Mitsuya, Yoko Nakasu, Ken Yamaguchi, Yasuto Akiyama
BACKGROUND: Temozolomide-resistant (TMZ-R) glioblastoma is very difficult to treat, and a novel approach to overcome resistance is needed. MATERIALS AND METHODS: The efficacy of a combination treatment of STAT3 inhibitor, STX-0119, with rapamycin was investigated against our established TMZ-resistant U87 cell line. RESULTS: The growth-inhibitory effect of the combination treatment was significant against the TMZ-R U87 cell line (IC50: 78 μM for STX-0119, 30...
2, 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28031239/micrornas-142-3p-mir-155-and-mir-203-are-deregulated-in-gastric-malt-lymphomas-compared-to-chronic-gastritis
#2
Concepción Fernández, Beatriz Bellosillo, Mariana Ferraro, Agustín Seoane, Blanca Sánchez-González, Silvia Pairet, Aina Pons, Luis Barranco, María Carmen Vela, Eva Gimeno, Lluís Colomo, Carles Besses, Alfons Navarro, Antonio Salar
BACKGROUND: Over the last years, our knowledge on pathogenesis of gastric MALT lymphoma has greatly improved, but its morphological diagnosis is still hampered by overlapping histological features with advanced chronic gastritis. MicroRNAs are deregulated in lymphomas, but their role and usefulness in gastric MALT lymphoma has not been extensively investigated. MATERIALS AND METHODS: We analyzed the expression of 384 miRNAs using TaqMan microRNA assay in a training series of 10 gastric MALT lymphomas, 3 chronic gastritis and 2 reactive lymph nodes...
2, 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28031238/customized-array-comparative-genomic-hybridization-analysis-of-25-phosphatase-encoding-genes-in-colorectal-cancer-tissues
#3
Izabela Laczmanska, Pawel Skiba, Pawel Karpinski, Marek Bebenek, Maria M Sasiadek
BACKGROUND/AIM: Molecular mechanisms of alterations in protein tyrosine phosphatases (PTPs) genes in cancer have been previously described and include chromosomal aberrations, gene mutations, and epigenetic silencing. However, little is known about small intragenic gains and losses that may lead to either changes in expression or enzyme activity and even loss of protein function. MATERIALS AND METHODS: The aim of this study was to investigate 25 phosphatase genes using customized array comparative genomic hybridization in 16 sporadic colorectal cancer tissues...
2, 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28031237/expression-profiling-of-a-human-thyroid-cell-line-stably-expressing-the-brafv600e-mutation
#4
Byoung-Ae Kim, Hyeon-Gun Jee, Jin Wook Yi, Su-Jin Kim, Young Jun Chai, June Young Choi, Kyu Eun Lee
BACKGROUND/AIM: The BRAF(V600E) mutation acts as an initiator of cancer development in papillary thyroid carcinoma (PTC). Gene expression changes caused by the BRAF(V600E) mutation may have an important role in thyroid cancer development. MATERIALS AND METHODS: To study genomic alterations caused by the BRAF(V600E) mutation, we made human thyroid cell lines that harbor the wild-type BRAF gene (Nthy/WT) and the V600E mutant-type BRAF gene (Nthy/V600E). RESULTS: Flow cytometry and western blotting showed stable transfection of the BRAF gene...
2, 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28031236/proteomics-analysis-of-ovarian-cancer-cell-lines-and-tissues-reveals-drug-resistance-associated-proteins
#5
Isa N Cruz, Helen M Coley, Holger B Kramer, Thumuluru Kavitah Madhuri, Nur A M Safuwan, Ana Rita Angelino, Min Yang
BACKGROUND: Carboplatin and paclitaxel form the cornerstone of chemotherapy for epithelial ovarian cancer, however, drug resistance to these agents continues to present challenges. Despite extensive research, the mechanisms underlying this resistance remain unclear. MATERIALS AND METHODS: A 2D-gel proteomics method was used to analyze protein expression levels of three human ovarian cancer cell lines and five biopsy samples. Representative proteins identified were validated via western immunoblotting...
2, 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28031235/htp-nutraceutical-screening-for-histone-deacetylase-inhibitors-and-effects-of-hdacis-on-tumor-suppressing-mirnas-by-trichostatin-a-and-grapeseed-vitis-vinifera-in-hela-cells
#6
Elizabeth A Mazzio, Karam F A Soliman
BACKGROUND/AIM: Aggressive tumor malignancies are a consequence of delayed diagnosis, epigenetic/phenotype changes and chemo-radiation resistance. Histone deacetylases (HDACs) are a major epigenetic regulator of transcriptional repression, which are highly overexpressed in advanced malignancy. While original chemotherapy drugs were modeled after phytochemicals elucidated by botanical screenings, HDAC inhibitors (HDACi) such as apicidin, trichostatin A (TSA) and butyrate were discovered as products of fungus and microbes, in particular, gut microbiota...
2, 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28031234/the-multiple-roles-of-exosomes-in-metastasis
#7
REVIEW
Ulrich H Weidle, Fabian Birzele, Gwen Kollmorgen, Rüdiger Rüger
Exosomes are important contributors to cell-cell communication and their role as diagnostic markers for cancer and the pathogenesis for cancer is under intensive investigation. Here, we focus on their role in metastasis-related processes. We discuss their impact regarding promotion of invasion and migration of tumor cells, conditioning of lymph nodes, generation of premetastatic niches and organotropism of metastasis. Furthermore, we highlight interactions of exosomes with bone marrow and stromal components such as fibroblasts, endothelial cells, myeloid- and other immune-related cells in the context of metastases...
2, 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807075/index
#8
(no author information available yet)
No abstract text is available yet for this article.
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807074/platelet-derived-growth-factor-bb-a-must-have-therapeutic-target-redivivus-in-ovarian-cancer
#9
Anca Maria Cimpean, Ionut Marcel Cobec, Raluca Amalia Ceaușu, Roxana Popescu, Anca Tudor, Marius Raica
BACKGROUND: We aimed to validate PDGF-BB protein expression by RNAscope, a sensitive method for PDGF-BB mRNA evaluation on paraffin embedded (FFPE) specimens of ovarian tumors. MATERIALS AND METHODS: Seventy-five FFPE ovarian cancer biopsies were assessed by immunohistochemistry followed by PDGF-BB mRNA RNAscope validation. RESULTS AND CONCLUSION: Dual PDGF-BB expression in tumor and stromal cells have been observed, being highly suggestive for PDGF-BB mediated stromal-tumor cells reciprocal interaction in ovarian cancer (p=0...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807073/archetype-and-rearranged-non-coding-control-regions-in-urothelial-bladder-carcinoma-of-immunocompetent-individuals
#10
Elena Anzivino, Maria Antonella Zingaropoli, Marco Iannetta, Valeria Antonietta Pietropaolo, Alessandra Oliva, Francesco Iori, Antonio Ciardi, Donatella Maria Rodio, Francesca Antonini, Cesare Giovanni Fedele, Alessandra D'Abramo, Claudio Maria Mastroianni, Vincenzo Vullo, Maria Rosa Ciardi
BACKGROUND: Polyomaviruses (PyVs) are potential transforming viruses. Despite their involvement in human tumours still being debated, there is evidence to suggest a role for PyVs in bladder carcinoma (BC). Therefore, a possible association between PyVs and BC was investigated. MATERIALS AND METHODS: Urine, blood and fresh bladder tissue specimens were collected from 29 patients with BC. PyV prevalence, non-coding control region (NCCR) organization and genotypic analysis were assessed...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807072/introduction-of-id2-enhances-invasiveness-in-id2-null-oral-squamous-cell-carcinoma-cells-via-the-snail-axis
#11
Y U Kamata, Tomoki Sumida, Yosuke Kobayashi, Akiko Ishikawa, Wataru Kumamaru, Yoshihide Mori
AIM: Inhibitor of DNA-binding (ID) proteins are negative regulators of basic helix-loop-helix transcription factors that generally stimulate cell proliferation and inhibit differentiation. However, the role of ID2 in cancer progression remains ambiguous. Here, we investigated the function of ID2 in ID2-null oral squamous cell carcinoma (OSCC) cells. MATERIALS AND METHODS: We introduced an ID2 cDNA construct into ID2-null OSCC cells and compared them with empty-vector-transfected cells in terms of cell proliferation, invasion, and activity and expression of matrix metalloproteinase (MMP)...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807071/association-between-type-specific-hpv-infections-and-htert-dna-methylation-in-patients-with-invasive-cervical-cancer
#12
Mónica Molano, Pablo Moreno-Acosta, Nicolás Morales, Marcela Burgos, Lina Buitrago, Oscar Gamboa, Rayner Alvarez, Suzanne M Garland, Sepehr N Tabrizi, Renske D M Steenbergen, Juan Carlos Mejía
BACKGROUND: There exists limited information on the role of hTERT methylation, and its association with type-specific HPV infections in cervical cancer. MATERIALS AND METHODS: Eighty-seven frozen samples were analyzed for type-specific HPV infection using a GP5(+)/GP6(+) PCR-RLB assay (RLB). hTERT DNA methylation analysis was performed using a newly developed PCR-RLB-hTERT. RESULTS: Ninety-three percent of samples were HPV-positive and fifteen different types were detected...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807070/association-of-bim-deletion-polymorphism-and-bim-%C3%AE-rna-expression-in-nsclc-with-egfr-mutation
#13
Kazutoshi Isobe, Atsushi Kakimoto, Tetsuo Mikami, Kyohei Kaburaki, Hiroshi Kobayashi, Takahiro Yoshizawa, Takashi Makino, Hajime Otsuka, G O Sano, Keishi Sugino, Susumu Sakamoto, Yujiro Takai, Naobumi Tochigi, Akira Iyoda, Sakae Homma
AIM: This pilot study assessed the association of BIM deletion polymorphism and BIM RNA isoform in patients with EGFR-positive non-small cell lung cancer (NSCLC). PATIENTS AND METHODS: The study included 33 patients with EGFR-positive NSCLC treated with gefitinib. BIM deletion polymorphism and BIM RNA isoform (EL/L/S/γ) were determined by polymerase chain reaction (PCR). RESULTS: BIM-γ expression was significantly higher in patients with BIM deletion polymorphism than among those without BIM deletion polymorphism inside tumors (p=0...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807069/a-comparative-study-of-the-molecular-characteristics-of-familial-gliomas-and-other-cancers
#14
Jie Lu, Mark G Burnett, Max Shpak
BACKGROUND: Familial cancers are those that co-occur among first-degree relatives without showing Mendelian patterns of inheritance. MATERIALS AND METHODS: In this analysis, we compare the genomic characteristics of familial and sporadic cancers, with a focus on low-grade gliomas (LGGs) using sequence and expression data from the Cancer Genome Atlas. RESULTS: Familial cancers show similar genomic and molecular biomarker profiles to sporadic cancers, consistent with the similarity in their clinical features...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807068/in-silico-analysis-validates-proteomic-findings-of-formalin-fixed-paraffin-embedded-cutaneous-squamous-cell-carcinoma-tissue
#15
Ali Azimi, Kimberley L Kaufman, Marina Ali, Steven Kossard, Pablo Fernandez-Penas
BACKGROUND: Cutaneous squamous cell carcinoma (cSCC) is a common type of skin cancer but there are no comprehensive proteomic studies on this entity. MATERIALS AND METHODS: We employed liquid chromatography coupled with tandem mass spectrometry (MS/MS) using formalin-fixed paraffin-embedded (FFPE) cSCC material to study the tumor and normal skin tissue proteomes. Ingenuity Pathway Analysis (IPA) was used to interpret the role of altered proteins in cSCC pathophysiology...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807067/characterization-of-heparan-sulfate-proteoglycan-positive-recycling-endosomes-isolated-from-glioma-cells
#16
Katarzyna A Podyma-Inoue, Takuya Moriwaki, Anupama R Rajapakshe, Kazue Terasawa, Miki Hara-Yokoyama
BACKGROUND: Heparan sulfate proteoglycans (HSPGs)-dependent endocytic events have been involved in glioma progression. Thus, comprehensive understanding of the intracellular trafficking complexes formed in presence of HSPGs would be important for development of glioma treatments. MATERIALS AND METHODS: Subcellular fractionation was used to separate vesicles containing HSPGs from the rat C6 glioma cell line. Isolated HSPG-positive vesicles were further characterized with liquid chromatography-mass spectrometry...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807066/differential-expression-of-ccn4-and-other-genes-between-metastatic-and-non-metastatic-el4-mouse-lymphoma-cells
#17
Manpreet S Chahal, H Teresa Ku, Zhihong Zhang, Christian M Legaspi, Angela Luo, Mandi M Hopkins, Kathryn E Meier
BACKGROUND: Previous work characterized variants of the EL4 murine lymphoma cell line. Some are non-metastatic, and others metastatic, in syngenic mice. In addition, metastatic EL4 cells were stably transfected with phospholipase D2 (PLD2), which further enhanced metastasis. MATERIALS AND METHODS: Microarray analyses of mRNA expression was performed for non-metastatic, metastatic, and PLD2-expressing metastatic EL4 cells. RESULTS: Many differences were observed between non-metastatic and metastatic cell lines...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807065/clinical-and-therapeutic-implications-of-follistatin-in-solid-tumours
#18
REVIEW
Lei Shi, Jeyna Resaul, Sioned Owen, Lin Ye, Wen G Jiang
Follistatin (FST), as a single-chain glycosylated protein, has two major isoforms, FST288 and FST315. The FST315 isoform is the predominant form whilst the FST288 variant accounts for less than 5% of the encoded mRNA. FST is differentially expressed in human tissues and aberrant expression has been observed in a variety of solid tumours, including gonadal, gastric and lung cancer, hepatocellular carcinoma, basal cell carcinoma and melanoma. Based on the current evidence, FST is an antagonist of transforming growth factor beta family members, such as activin and bone morphogenetic proteins (BMPs)...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27807064/mechanisms-and-targets-involved-in-dissemination-of-ovarian-cancer
#19
REVIEW
Ulrich H Weidle, Fabian Birzele, Gwendlyn Kollmorgen, Rüdiger Rueger
Ovarian carcinoma is associated with the highest death rate of all gynecological tumors. On one hand, its aggressiveness is based on the rapid dissemination of ovarian cancer cells to the peritoneum, the omentum, and organs located in the peritoneal cavity, and on the other hand, on the rapid development of resistance to chemotherapeutic agents. In this review, we focus on the metastatic process of ovarian cancer, which involves dissemination of, homing to and growth of tumor cells in distant organs, and describe promising molecular targets for possible therapeutic intervention...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27566658/enhancement-of-pathologist-s-routine-practice-reuse-of-dna-extracted-from-immunostained-formalin-fixed-paraffin-embedded-ffpe-slides-in-downstream-molecular-analysis-of-cancer
#20
Asmaa Al-Attas, Mourad Assidi, Jaudah Al-Maghrabi, Ashraf Dallol, Hans-Juergen Schulten, Muhammad Abu-Elmagd, Adeel Chaudhary, Adel Abuzenadah, Bruce Budowle, Abdelbaset Buhmeida, Mohammed Al-Qahtani
BACKGROUND/AIM: To date, the conventional formalin-fixed, paraffin-embedded (FFPE) technique is the gold-standard for preserving histomorphology. Once FFPE tissues are stained, slides are routinely archived along with their blocks at biobanks/hospitals. However, the reuse of fixed and stained biospecimens as DNA source is not a common routine practice worldwide and, thus, indicates the need of studies to investigate the feasibility of extracting DNA from already immunohistochemistry (IHC) FFPE-stained slides and its possible reuse in subsequent downstream molecular analyses...
September 2016: Cancer Genomics & Proteomics
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