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Clinical Proteomics

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https://www.readbyqxmd.com/read/28701906/proteomic-discovery-and-verification-of-serum-amyloid-a-as-a-predictor-marker-of-patients-at-risk-of-post-stroke-infection-a-pilot-study
#1
L Azurmendi, V Lapierre-Fetaud, J Schneider, J Montaner, M Katan, Jean-Charles Sanchez
BACKGROUND: Post-stroke infections occur in 20-36% of stroke patients and are associated with high morbidity and mortality rates. Early identification of patients at risk of developing an infection could improve care via an earlier treatment leading to a better outcome. We used proteomic tools in order to discover biomarkers able to stratify patients at risk of post-stroke infection. METHODS: The post hoc analysis of a prospective cohort study including 40 ischemic stroke patients included 21 infected and 19 non-infected participants...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28694743/interferon-%C3%AE-induced-compositional-changes-in-human-bone-marrow-derived-mesenchymal-stem-stromal-cells
#2
Qingdong Guan, Peyman Ezzati, Victor Spicer, Oleg Krokhin, Donna Wall, John A Wilkins
BACKGROUND: Mesenchymal stem/stromal cells (MSC) display a range of immunoregulatory properties which can be enhanced by the exposure to cytokines such interferon γ (IFN-γ). However the compositional changes associated with the 'licensing' of these cells have not been clearly defined. The present study was undertaken to provide a detailed comparative proteomic analysis of the compositional changes that occur in human bone marrow derived MSC following 20 h treatment with IFN-γ. METHODS: 2D LC MSMS analysis of control and IFN-γ treated cells from 5 different healthy donors provided confident identification of more than 8400 proteins...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28694742/clinical-validation-of-a-blood-based-classifier-for-diagnostic-evaluation-of-asymptomatic-individuals-with-pulmonary-nodules
#3
Charles E Birse, Jennifer L Tomic, Harvey I Pass, William N Rom, Robert J Lagier
BACKGROUND: The number of pulmonary nodules detected in the US is expected to increase substantially following recent recommendations for nationwide CT-based lung cancer screening. Given the low specificity of CT screening, non-invasive adjuvant methods are needed to differentiate cancerous lesions from benign nodules to help avoid unnecessary invasive procedures in the asymptomatic population. We have constructed a serum-based multi-biomarker panel and assessed its clinical accuracy in a retrospective analysis of samples collected from participants with suspicious radiographic findings in the Prostate, Lung, Chest and Ovarian (PLCO) cancer screening trial...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28670222/the-impact-of-growth-hormone-on-proteomic-profiles-a-review-of-mouse-and-adult-human-studies
#4
REVIEW
Silvana Duran-Ortiz, Alison L Brittain, John J Kopchick
Growth hormone (GH) is a protein that is known to stimulate postnatal growth, counter regulate insulin's action and induce expression of insulin-like growth factor-1. GH exerts anabolic or catabolic effects depending upon on the targeted tissue. For instance, GH increases skeletal muscle and decreases adipose tissue mass. Our laboratory has spent the past two decades studying these effects, including the effects of GH excess and depletion, on the proteome of several mouse and human tissues. This review first discusses proteomic techniques that are commonly used for these types of studies...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28649187/distinct-proteome-pathology-of-circulating-microparticles-in-systemic-lupus-erythematosus
#5
Ole Østergaard, Christoffer Tandrup Nielsen, Julia T Tanassi, Line V Iversen, Søren Jacobsen, Niels H H Heegaard
BACKGROUND: The pathogenesis of systemic lupus erythematosus (SLE) is poorly understood but has been linked to defective clearance of subcellular particulate material from the circulation. This study investigates the origin, formation, and specificity of circulating microparticles (MPs) in patients with SLE based on comprehensive MP proteome profiling using patients with systemic sclerosis (SSc) and healthy donors (HC) as controls. METHODS: We purified MPs from platelet-poor plasma using differential centrifugation of samples from SLE (n = 45), SSc (n = 38), and two sets of HC (n = 35, n = 25)...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28642677/a-novel-workflow-combining-plaque-imaging-plaque-and-plasma-proteomics-identifies-biomarkers-of-human-coronary-atherosclerotic-plaque-disruption
#6
Regent Lee, Roman Fischer, Philip D Charles, David Adlam, Alessandro Valli, Katalin Di Gleria, Rajesh K Kharbanda, Robin P Choudhury, Charalambos Antoniades, Benedikt M Kessler, Keith M Channon
BACKGROUND: Atherosclerotic plaque rupture is the culprit event which underpins most acute vascular syndromes such as acute myocardial infarction. Novel biomarkers of plaque rupture could improve biological understanding and clinical management of patients presenting with possible acute vascular syndromes but such biomarker(s) remain elusive. Investigation of biomarkers in the context of de novo plaque rupture in humans is confounded by the inability to attribute the plaque rupture as the source of biomarker release, as plaque ruptures are typically associated with prompt down-stream events of myocardial necrosis and systemic inflammation...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28592925/second-generation-multiple-reaction-monitoring-assays-for-enhanced-detection-of-ultra-low-abundance-mycobacterium-tuberculosis-peptides-in-human-serum
#7
Carolina Mehaffy, Karen M Dobos, Payam Nahid, Nicole A Kruh-Garcia
BACKGROUND: Mycobacterium tuberculosis (Mtb) is the causative agent of Tuberculosis (TB), the number one cause of death due to an infectious disease. TB diagnosis is performed by microscopy, culture or PCR amplification of bacterial DNA, all of which require patient sputum or the biopsy of infected tissue. Detection of mycobacterial products in serum, as biomarkers of diagnosis or disease status would provide an improvement over current methods. Due to the low-abundance of mycobacterial products in serum, we have explored exosome enrichment to improve sensitivity...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28546799/lectin-array-and-glycogene-expression-analyses-of-ovarian-cancer-cell-line-a2780-and-its-cisplatin-resistant-derivate-cell-line-a2780-cp
#8
Ran Zhao, Wenjun Qin, Ruihuan Qin, Jing Han, Can Li, Yisheng Wang, Congjian Xu
BACKGROUND: Ovarian cancer is one of the most lethal gynecological malignancies, in which platinum resistance is a common cause of its relapse and death. Glycosylation has been reported to be involved in drug resistance, and glycomic analyses of ovarian cancer may improve our understanding of the mechanisms underlying cancer cell drug resistance and provide potential biomarkers and therapeutic targets. METHODS: The serous ovarian cancer cell line A2780 and its platinum-resistant counterpart A2780-cp were used in this study...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28529466/the-serum-protein-responses-to-treatment-with-xiaoke-pill-and-glibenclamide-in-type-2-diabetes-patients
#9
Xiuying Zhang, Haidan Sun, Sanjoy K Paul, Quanhui Wang, Xiaomin Lou, Guixue Hou, Bo Wen, Linong Ji, Siqi Liu
AIM: The Xiaoke Pill containing Chinese herb extracts and Glibenclamide, is used in therapy for type 2 diabetes mellitus (T2DM), and is effective in reducing the risk of hypoglycemia and improving diabetes symptoms compared with Glibenclamide. We describe a quantitative proteomics project to measure the T2DM serum proteome response to the Xiaoke Pill and Glibenclamide. METHODS: Based on a recently conducted 48-week clinical trial comparing the safety and efficacy of Glibenclamide (n = 400) and Xiaoke Pill (n = 400), after matching for age, sex, BMI, drug dose and whether hypoglycemia occurred, 32 patients were selected for the serum based proteomic analysis and divided into four groups (with/without hypoglycemia treated with Xiaoke Pill or Glibenclamide, n = 8 for each group)...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28522940/potential-urine-proteomics-biomarkers-for-primary-nephrotic-syndrome
#10
Young Wook Choi, Yang Gyun Kim, Min-Young Song, Ju-Young Moon, Kyung-Hwan Jeong, Tae-Won Lee, Chun-Gyoo Ihm, Kang-Sik Park, Sang-Ho Lee
BACKGROUND: Nephrotic syndrome (NS) is a nonspecific kidney disorder, commonly caused by minimal change disease (MCD), focal segmental glomerulosclerosis (FSGS), and membranous nephropathy (MN). Here we analyzed urinary protein profiles, aiming to discover disease-specific biomarkers of these three common diseases in NS. METHODS: Sixteen urine samples were collected from patients with biopsy-proven NS and healthy controls. After removal of high-abundance proteins, the urinary protein profile was analyzed by LC-MS/MS to generate a discovery set...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28522939/identification-of-protein-targets-in-cerebral-endothelial-cells-for-brain-arteriovenous-malformation-avms-molecular-therapies
#11
Margaret Simonian, Rachel R Ogorzalek Loo, Nalaka Rannulu, Joseph A Loo, Mark P Molloy, Marcus A Stoodley
BACKGROUND: To develop a new molecular targeted treatment for brain (AVMs), identification of membrane proteins that are localised on the AVM endothelium is crucial. Current treatment methods are surgery and radiosurgery. However, complete occlusion post radiosurgery are achieved within 3 years, while patient remain at risk of haemorrhage. This study aims to identify potential protein targets in AVM endothelial cells that discriminate these vessels from normal vessels; these proteins targets will be investigated for the molecular therapy of brain AVMs to promote rapid thrombosis after radiosurgery...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28491011/an-integrated-proteomic-and-glycoproteomic-approach-uncovers-differences-in-glycosylation-occupancy-from-benign-and-malignant-epithelial-ovarian-tumors
#12
Qing Kay Li, Punit Shah, Yuan Tian, Yingwei Hu, Richard B S Roden, Hui Zhang, Daniel W Chan
BACKGROUND: Epithelial ovarian carcinomas encompass a heterogeneous group of diseases with a poor 5-year survival rate. Serous carcinoma is the most common type. Most FDA-approved serum tumor markers are glycoproteins. These glycoproteins on cell surface or shed into the bloodstream could serve as therapeutic targets as well as surrogates of tumor. In addition to glycoprotein expressions, the analysis of protein glycosylation occupancy could be important for the understanding of cancer biology as well as the identification of potential glycoprotein changes in cancer...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28484360/downregulation-of-atp1a1-promotes-cancer-development-in-renal-cell-carcinoma
#13
Dan Zhang, Peng Zhang, Pengbo Yang, Yu He, Xixi Wang, Yanfang Yang, Hongxia Zhu, Ningzhi Xu, Shufang Liang
BACKGROUND: Aberrant expression of Na(+)/K(+)-ATPase α1 subunit (ATP1A1) is widely observed in multiple types of tumors, and its tissue-specific expression relates to cancer development. However, the functions and molecular mechanisms in renal cell carcinoma (RCC) are not fully understood. METHODS: We investigated the ATP1A1 expression changes and possible roles in RCC through a quantitative proteomic approach and an integrative biochemical assessment. We detected ATP1A1 in RCC with LC-MS/MS, and further validated its expression with immunohistochemical analyses of 80 pairs of the RCC tumor and non-tumor tissues samples...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28450824/identification-of-novel-biomarker-and-therapeutic-target-candidates-for-acute-intracerebral-hemorrhage-by-quantitative-plasma-proteomics
#14
Guo-Chun Li, Lina Zhang, Ming Yu, Haiyu Jia, Ting Tian, Junqin Wang, Fuqiang Wang, Ling Zhou
BACKGROUND: The systematic mechanisms of acute intracerebral hemorrhage are still unknown and unverified, although many recent researches have indicated the secondary insults. This study was aimed to disclose the pathological mechanism and identify novel biomarker and therapeutic target candidates by plasma proteome. METHODS: Patients with AICH (n = 8) who demographically matched healthy controls (n = 4) were prospectively enrolled, and their plasma samples were obtained...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28450823/proteomic-profiling-of-human-intraschisis-cavity-fluid
#15
Dhandayuthapani Sudha, Mahdokht Kohansal-Nodehi, Purnima Kovuri, Srikanth Srinivas Manda, Srividya Neriyanuri, Lingam Gopal, Pramod Bhende, Subbulakshmi Chidambaram, Jayamuruga Pandian Arunachalam
BACKGROUND: X-linked retinoschisis (XLRS) is a vitreoretinal degenerative disorder causing vision deterioration, due to structural defects in retina. The hallmark of this disease includes radial streaks arising from the fovea and splitting of inner retinal layers (schisis). Although these retinal changes are attributed to mutations in the retinoschisin gene, schisis is also observed in patients who do not carry mutations. In addition, the origin of intraschisis fluid, the triggering point of schisis formation and its progression are largely unknown still...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28439213/a-clinical-perspective-on-the-utility-of-alpha-1-antichymotrypsin-for-the-early-diagnosis-of-calcific-aortic-stenosis
#16
Tatiana Martin-Rojas, Laura Mourino-Alvarez, Felix Gil-Dones, Fernando de la Cuesta, Esther Rosello-Lleti, Carlos M Laborde, Miguel Rivera, Luis Fernando Lopez-Almodovar, Juan Antonio Lopez, Finn Akerstrom, Luis R Padial, Maria G Barderas
BACKGROUND: Calcific aortic stenosis (CAS) is the most common heart valve disease in the elderly, representing an important economic and social burden in developed countries. Currently, there is no way to predict either the onset or progression of CAS, emphasizing the need to identify useful biomarkers for this condition. METHODS: We performed a multi-proteomic analysis on different kinds of samples from CAS patients and healthy donors: tissue, secretome and plasma...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28405179/a-review-of-studies-of-the-proteomes-of-circulating-microparticles-key-roles-for-galectin-3-binding-protein-expressing-microparticles-in-vascular-diseases-and-systemic-lupus-erythematosus
#17
REVIEW
Christoffer T Nielsen, Ole Østergaard, Niclas S Rasmussen, Søren Jacobsen, Niels H H Heegaard
Subcellular microvesicles (MVs) have attracted increasing interest during the past decades. While initially considered as inert cellular debris, several important roles for MVs in physiological homeostasis, cancer, cardiovascular, and autoimmune diseases have been uncovered. Although still poorly understood, MVs are involved in trafficking of information from cell-to-cell, and are implicated in the regulation of immunity, thrombosis, and coagulation. Different subtypes of extracellular MVs exist. This review focuses on the cell membrane-derived shedded MVs (ranging in size from 200 to 1000 nm) typically termed microparticles (MPs)...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28360826/glycated-lysine-141-in-haptoglobin-improves-the-diagnostic-accuracy-for-type-2-diabetes-mellitus-in-combination-with-glycated-hemoglobin-hba1c-and-fasting-plasma-glucose
#18
Sandro Spiller, Yichao Li, Matthias Blüher, Lonnie Welch, Ralf Hoffmann
BACKGROUND: Recent epidemiological studies indicate that only 30-50% of undiagnosed type 2 diabetes mellitus (T2DM) patients are identified using glycated hemoglobin (HbA1c) and elevated fasting plasma glucose (FPG) levels. Thus, novel biomarkers for early diagnosis and prognosis are urgently needed for providing early and personalized treatment. METHODS: Here, we studied the glycation degrees of 27 glycation sites representing nine plasma proteins in 48 newly diagnosed male T2DM patients and 48 non-diabetic men matched for age (range 35-65 years)...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28344541/olfm4-kng1-and-sec24c-identified-by-proteomics-and-immunohistochemistry-as-potential-markers-of-early-colorectal-cancer-stages
#19
Florence Quesada-Calvo, Charlotte Massot, Virginie Bertrand, Rémi Longuespée, Noëlla Blétard, Joan Somja, Gabriel Mazzucchelli, Nicolas Smargiasso, Dominique Baiwir, Marie-Claire De Pauw-Gillet, Philippe Delvenne, Michel Malaise, Carla Coimbra Marques, Marc Polus, Edwin De Pauw, Marie-Alice Meuwis, Edouard Louis
BACKGROUND: Despite recent advances in colorectal cancer (CRC) diagnosis and population screening programs, the identification of patients with preneoplastic lesions or with early CRC stages remains challenging and is important for reducing CRC incidence and increasing patient's survival. METHODS: We analysed 76 colorectal tissue samples originated from early CRC stages, normal or inflamed mucosa by label-free proteomics. The characterisation of three selected biomarker candidates was performed by immunohistochemistry on an independent set of precancerous and cancerous lesions harbouring increasing CRC stages...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28344540/tubulointerstitial-nephritis-antigen-like-1-protein-is-downregulated-in-the-placenta-of-pre-eclamptic-women
#20
Sheon Mary, Mahesh J Kulkarni, Savita S Mehendale, Sadhana R Joshi, Ashok P Giri
BACKGROUND: Tubulointerstitial nephritis antigen-like 1 protein (TINAGL1), is a matricellular protein, known to play role in cell adhesion and cell receptor interaction. Research related to TINAGL1 is limited to cell culture and animal models. Demonstration of TINAGL1 as a positive regulator of angiogenesis and its expression in the decidua of postimplantation mouse uterus, prompted us to validate its expression in human placenta during impaired angiogenesis in pre-eclamptic condition...
2017: Clinical Proteomics
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