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Clinical Proteomics

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https://www.readbyqxmd.com/read/28405179/a-review-of-studies-of-the-proteomes-of-circulating-microparticles-key-roles-for-galectin-3-binding-protein-expressing-microparticles-in-vascular-diseases-and-systemic-lupus-erythematosus
#1
REVIEW
Christoffer T Nielsen, Ole Østergaard, Niclas S Rasmussen, Søren Jacobsen, Niels H H Heegaard
Subcellular microvesicles (MVs) have attracted increasing interest during the past decades. While initially considered as inert cellular debris, several important roles for MVs in physiological homeostasis, cancer, cardiovascular, and autoimmune diseases have been uncovered. Although still poorly understood, MVs are involved in trafficking of information from cell-to-cell, and are implicated in the regulation of immunity, thrombosis, and coagulation. Different subtypes of extracellular MVs exist. This review focuses on the cell membrane-derived shedded MVs (ranging in size from 200 to 1000 nm) typically termed microparticles (MPs)...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28360826/glycated-lysine-141-in-haptoglobin-improves-the-diagnostic-accuracy-for-type-2-diabetes-mellitus-in-combination-with-glycated-hemoglobin-hba1c-and-fasting-plasma-glucose
#2
Sandro Spiller, Yichao Li, Matthias Blüher, Lonnie Welch, Ralf Hoffmann
BACKGROUND: Recent epidemiological studies indicate that only 30-50% of undiagnosed type 2 diabetes mellitus (T2DM) patients are identified using glycated hemoglobin (HbA1c) and elevated fasting plasma glucose (FPG) levels. Thus, novel biomarkers for early diagnosis and prognosis are urgently needed for providing early and personalized treatment. METHODS: Here, we studied the glycation degrees of 27 glycation sites representing nine plasma proteins in 48 newly diagnosed male T2DM patients and 48 non-diabetic men matched for age (range 35-65 years)...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28344541/olfm4-kng1-and-sec24c-identified-by-proteomics-and-immunohistochemistry-as-potential-markers-of-early-colorectal-cancer-stages
#3
Florence Quesada-Calvo, Charlotte Massot, Virginie Bertrand, Rémi Longuespée, Noëlla Blétard, Joan Somja, Gabriel Mazzucchelli, Nicolas Smargiasso, Dominique Baiwir, Marie-Claire De Pauw-Gillet, Philippe Delvenne, Michel Malaise, Carla Coimbra Marques, Marc Polus, Edwin De Pauw, Marie-Alice Meuwis, Edouard Louis
BACKGROUND: Despite recent advances in colorectal cancer (CRC) diagnosis and population screening programs, the identification of patients with preneoplastic lesions or with early CRC stages remains challenging and is important for reducing CRC incidence and increasing patient's survival. METHODS: We analysed 76 colorectal tissue samples originated from early CRC stages, normal or inflamed mucosa by label-free proteomics. The characterisation of three selected biomarker candidates was performed by immunohistochemistry on an independent set of precancerous and cancerous lesions harbouring increasing CRC stages...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28344540/tubulointerstitial-nephritis-antigen-like-1-protein-is-downregulated-in-the-placenta-of-pre-eclamptic-women
#4
Sheon Mary, Mahesh J Kulkarni, Savita S Mehendale, Sadhana R Joshi, Ashok P Giri
BACKGROUND: Tubulointerstitial nephritis antigen-like 1 protein (TINAGL1), is a matricellular protein, known to play role in cell adhesion and cell receptor interaction. Research related to TINAGL1 is limited to cell culture and animal models. Demonstration of TINAGL1 as a positive regulator of angiogenesis and its expression in the decidua of postimplantation mouse uterus, prompted us to validate its expression in human placenta during impaired angiogenesis in pre-eclamptic condition...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28184180/serum-proteomic-based-analysis-identifying-autoantibodies-against-prdx2-and-prdx3-as-potential-diagnostic-biomarkers-in-nasopharyngeal-carcinoma
#5
Lie-Hao Lin, Yi-Wei Xu, Li-Sheng Huang, Chao-Qun Hong, Tian-Tian Zhai, Lian-Di Liao, Wen-Jie Lin, Li-Yan Xu, Kai Zhang, En-Min Li, Yu-Hui Peng
BACKGROUND: Nasopharyngeal carcinoma (NPC) is a major head and neck cancer with high occurrence in Southeast Asia and southern China. We aimed to identify autoantibodies that may contribute to early detection of NPC. METHODS: We used serological proteome analysis to identify candidate autoantibodies against tumor-associated antigens. Levels of autoantibodies and Epstein-Barr virus capsid antigen-IgA (VCA-IgA) were measured by ELISA in 129 patients with NPC and 100 normal controls...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28174513/cervical-mucus-proteome-in-endometriosis
#6
Giuseppe Grande, Federica Vincenzoni, Domenico Milardi, Giuseppina Pompa, Domenico Ricciardi, Erika Fruscella, Francesca Mancini, Alfredo Pontecorvi, Massimo Castagnola, Riccardo Marana
BACKGROUND: Endometriosis is a chronic gynecological inflammatory disease characterized by the presence of functional endometrial glands and stroma outside of the uterine cavity. It affects 7-10% of women of reproductive age and up to 50% of women with infertility. The current gold standard for the diagnosis combines laparoscopic evaluation and biopsy of the visualized lesions. However, laparoscopy requires general anesthesia and developed surgical skills and it has a high procedural cost...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28115918/immune-enrichment-of-insulin-in-bio-fluids-on-gold-nanoparticle-decorated-target-plate-and-in-situ-detection-by-maldi-ms
#7
Kai Liang, Hongmei Wu, Yan Li
BACKGROUND: Detection of low-abundance biomarkers using mass spectrometry (MS) is often hampered by non-target molecules in biological fluids. In addition, current procedures for sample preparation increase sample consumption and limit analysis throughput. Here, a simple strategy is proposed to construct an antibody-modified target plate for high-sensitivity MS detection of target markers such as insulin, in biological fluids. METHODS: The target plate was first modified with gold nanoparticle, and then functionalized with corresponding antibody through chemical conjugation...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28115917/a-new-enzyme-linked-immunosorbent-assay-elisa-for-human-free-and-bound-kallikrein-9
#8
Panagiota Filippou, Dimitrios Korbakis, Sofia Farkona, Antoninus Soosaipillai, Theano Karakosta, Eleftherios P Diamandis
BACKGROUND: Kallikrein 9 (KLK9) is a member of the human kallikrein-related peptidases family, whose physiological role and implications in disease processes remain unclear. The active form of the enzyme is predicted to have chymotryptic activity. In the present study, we produced for the first time the active recombinant protein and monoclonal antibodies, and developed novel immunoassays for the quantification of free and bound KLK9 in biological samples. METHODS: The coding sequence of mature KLK9 isoform (mat-KLK9) was expressed in an Expi293F mammalian system and the synthesized polypeptide was purified through a two-step protocol...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28100988/simultaneous-analyses-of-n-linked-and-o-linked-glycans-of-ovarian-cancer-cells-using-solid-phase-chemoenzymatic-method
#9
Shuang Yang, Naseruddin Höti, Weiming Yang, Yang Liu, Lijun Chen, Shuwei Li, Hui Zhang
BACKGROUND: Glycans play critical roles in a number of biological activities. Two common types of glycans, N-linked and O-linked, have been extensively analyzed in the last decades. N-glycans are typically released from glycoproteins by enzymes, while O-glycans are released from glycoproteins by chemical methods. It is important to identify and quantify both N- and O-linked glycans of glycoproteins to determine the changes of glycans. METHODS: The effort has been dedicated to study glycans from ovarian cancer cells treated with O-linked glycosylation inhibitor qualitatively and quantitatively...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28077936/redox-proteomic-identification-of-carbonylated-proteins-in-autism-plasma-insight-into-oxidative-stress-and-its-related-biomarkers-in-autism
#10
Chengyun Feng, Youjiao Chen, Jintao Pan, Aochu Yang, Li Niu, Jie Min, Xianling Meng, Liping Liao, Kaoyuan Zhang, Liming Shen
BACKGROUND: Autism is a severe childhood neurological disorder with poorly understood etiology and pathology. Currently, there is no authentic laboratory test to confirm the diagnosis of autism. Oxidative damage may play a central role in the pathogenesis of autism. Present study is an effort to search for possible biomarkers of autism and further clarify the molecular changes associated with oxidative stress that occurs in the plasma of autistic children. METHODS: We performed redox proteomics analysis to compare carbonylated proteins in the plasma of autistic subjects and healthy controls...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28077935/development-of-a-microarray-based-method-for-allergen-specific-ige-and-igg4-detection
#11
Guzel Feyzkhanova, Sergei Voloshin, Olga Smoldovskaya, Alla Arefieva, Marina Filippova, Viktor Barsky, Ludmila Pavlushkina, Veronika Butvilovskaya, Alexei Tikhonov, Yuri Reznikov, Alla Rubina
BACKGROUND: sIgE and sIgG4 detection is necessary for more accurate and effective type I hypersensitivity diagnostics and the estimation of disease development. Typically, the analyses of these antibodies are performed separately with the help of various specialized systems. The aim of this study was to develop a microarray-based method for the simultaneous quantitative detection of sIgE and sIgG4 to the most common allergens in a single sample. METHODS: A quantitative method for the simultaneous detection of sIgE and sIgG4 was developed based on the technology of hydrogel microchips previously designed at Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS)...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/27980500/cardiovascular-proteomics-in-the-era-of-big-data-experimental-and-computational-advances
#12
REVIEW
Maggie P Y Lam, Edward Lau, Dominic C M Ng, Ding Wang, Peipei Ping
Proteomics plays an increasingly important role in our quest to understand cardiovascular biology. Fueled by analytical and computational advances in the past decade, proteomics applications can now go beyond merely inventorying protein species, and address sophisticated questions on cardiac physiology. The advent of massive mass spectrometry datasets has in turn led to increasing intersection between proteomics and big data science. Here we review new frontiers in technological developments and their applications to cardiovascular medicine...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27895544/mesoporous-silica-chip-enabled-peptide-profiling-as-an-effective-platform-for-controlling-bio-sample-quality-and-optimizing-handling-procedure
#13
Kai Liang, Hongmei Wu, Tony Y Hu, Yan Li
BACKGROUND: High quality clinical samples are critical for meaningful interpretation of data obtained in both basic and translational medicine. More specifically, optimized pre-analysis handling to bio-sample is crucial for avoiding biased analysis in a clinical setting. A universally applicable method for the evaluation of sample quality and pre-analysis handling is therefore in great demand. METHODS: The fingerprint pattern of low molecular weight (LMW) peptides in sera is directly associated with sample quality and handling process...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27895543/proteome-analysis-of-excretory-secretory-proteins-of-entamoeba-histolytica-hm1-imss-via-lc-esi-ms-ms-and-lc-maldi-tof-tof
#14
Jorim Anak Ujang, Soon Hong Kwan, Mohd Nazri Ismail, Boon Huat Lim, Rahmah Noordin, Nurulhasanah Othman
BACKGROUND: Excretory-secretory (ES) proteins of E. histolytica are thought to play important roles in the host invasion, metabolism, and defence. Elucidation of the types and functions of E. histolytica ES proteins can further our understanding of the disease pathogenesis. Thus, the aim of this study is to use proteomics approach to better understand the complex ES proteins of the protozoa. METHODS: E. histolytica ES proteins were prepared by culturing the trophozoites in protein-free medium...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27833472/integrated-glycomic-analysis-of-ovarian-cancer-side-population-cells
#15
Ran Zhao, Xiaoxia Liu, Yisheng Wang, Xiaoxiang Jie, Ruihuan Qin, Wenjun Qin, Mengyu Zhang, Haiyan Tai, Caiting Yang, Lili Li, Peike Peng, Miaomiao Shao, Xingwang Zhang, Hao Wu, Yuanyuan Ruan, Congjian Xu, Shifang Ren, Jianxin Gu
BACKGROUND: Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment. Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences. A better understanding of SP cells may provide new clues for detecting and targeting these cancer-initiating cells and ultimately help to eradicate cancer...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27822170/proteomics-in-india-the-clinical-aspect
#16
REVIEW
Somaditya Mukherjee, Arun Bandyopadhyay
Proteomics has emerged as a highly promising bioanalytical technique in various aspects of applied biological research. In Indian academia, proteomics research has grown remarkably over the last decade. It is being extensively used for both basic as well as translation research in the areas of infectious and immune disorders, reproductive disorders, cardiovascular diseases, diabetes, eye disorders, human cancers and hematological disorders. Recently, some seminal works on clinical proteomics have been reported from several laboratories across India...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27799870/proteomic-profiling-of-lung-adenocarcinoma-indicates-heightened-dna-repair-antioxidant-mechanisms-and-identifies-lasp1-as-a-potential-negative-predictor-of-survival
#17
Johannes F Fahrmann, Dmitry Grapov, Brett S Phinney, Carol Stroble, Brian C DeFelice, William Rom, David R Gandara, Yanhong Zhang, Oliver Fiehn, Harvey Pass, Suzanne Miyamoto
BACKGROUND: Lung cancer is the leading cause of cancer mortality in the United States. Non-small cell lung cancer accounts for 85% of all lung cancers for which adenocarcinoma is the most common histological type. Management of lung cancer is hindered by high false-positive rates due to difficulty resolving between benign and malignant tumors. Better molecular analysis comparing malignant and non-malignant tissues will provide important evidence of the underlying biology contributing to tumorigenesis...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27799869/proteomic-profiling-of-retinoblastoma-by-high-resolution-mass-spectrometry
#18
Ravikanth Danda, Kalaivani Ganapathy, Gajanan Sathe, Anil K Madugundu, Sharavan Ramachandran, Uma Maheswari Krishnan, Vikas Khetan, Pukhraj Rishi, T S Keshava Prasad, Akhilesh Pandey, Subramanian Krishnakumar, Harsha Gowda, Sailaja V Elchuri
BACKGROUND: Retinoblastoma is an ocular neoplastic cancer caused primarily due to the mutation/deletion of RB1 gene. Due to the rarity of the disease very limited information is available on molecular changes in primary retinoblastoma. High throughput analysis of retinoblastoma transcriptome is available however the proteomic landscape of retinoblastoma remains unexplored. In the present study we used high resolution mass spectrometry-based quantitative proteomics to identify proteins associated with pathogenesis of retinoblastoma...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27799868/antibody-based-proteomics-to-identify-an-apoptosis-signature-for-early-recurrence-of-hepatocellular-carcinoma
#19
Noriaki Morofuji, Hidenori Ojima, Nobuyoshi Hiraoka, Takuji Okusaka, Minoru Esaki, Satoshi Nara, Kazuaki Shimada, Yoshiro Kishi, Tadashi Kondo
BACKGROUND: Early recurrence after surgical resection is a hallmark of poor prognosis in hepatocellular carcinoma (HCC). To determine the proteomic background of early recurrence of HCC, we focused on apoptosis-related proteins. METHODS: Surgically resected tumor tissues were obtained from 80 patients, including HCC tumor tissues, non-tumor tissues, and normal liver tissues. These samples were grouped in the discovery and validation sample sets. The expression level of 192 apoptosis-related proteins was monitored using 247 commercially available antibodies and western blotting...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27795698/implementation-and-evaluation-of-amyloidosis-subtyping-by-laser-capture-microdissection-and-tandem-mass-spectrometry
#20
Peter Mollee, Samuel Boros, Dorothy Loo, Jayde E Ruelcke, Vanessa A Lakis, Kim-Anh Lê Cao, Patricia Renaut, Michelle M Hill
BACKGROUND: Correct identification of the amyloidosis-causing protein is crucial for clinical management. Recently the Mayo Clinic reported laser-capture microdissection (LCM) with liquid chromatography-coupled tandem mass spectrometry (MS/MS) as a new diagnostic tool for amyloid diagnosis. Here, we report an independent implementation of this proteomic diagnostics method at the Princess Alexandra Hospital Amyloidosis Centre in Brisbane, Australia. RESULTS: From 2010 to 2014, 138 biopsies received from 35 different organ sites were analysed by LCM-MS/MS using Congo Red staining to visualise amyloid deposits...
2016: Clinical Proteomics
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