journal
https://read.qxmd.com/read/38547242/mycn-drives-oncogenesis-by-cooperating-with-the-histone-methyltransferase-g9a-and-the-wdr5-adaptor-to-orchestrate-global-gene-transcription
#1
JOURNAL ARTICLE
Zhihui Liu, Xiyuan Zhang, Man Xu, Jason J Hong, Amanda Ciardiello, Haiyan Lei, Jack F Shern, Carol J Thiele
MYCN activates canonical MYC targets involved in ribosome biogenesis, protein synthesis, and represses neuronal differentiation genes to drive oncogenesis in neuroblastoma (NB). How MYCN orchestrates global gene expression remains incompletely understood. Our study finds that MYCN binds promoters to up-regulate canonical MYC targets but binds to both enhancers and promoters to repress differentiation genes. MYCN binding also increases H3K4me3 and H3K27ac on canonical MYC target promoters and decreases H3K27ac on neuronal differentiation gene enhancers and promoters...
March 28, 2024: PLoS Biology
https://read.qxmd.com/read/38547237/the-mechanism-of-cytoplasmic-incompatibility-is-conserved-in-wolbachia-bearing-aedes-aegypti-mosquitoes-deployed-for-arbovirus-control
#2
JOURNAL ARTICLE
Rupinder Kaur, Cole J Meier, Elizabeth A McGraw, Julian F Hillyer, Seth R Bordenstein
The rising interest and success in deploying inherited microorganisms and cytoplasmic incompatibility (CI) for vector control strategies necessitate an explanation of the CI mechanism. Wolbachia-induced CI manifests in the form of embryonic lethality when sperm from Wolbachia-bearing testes fertilize eggs from uninfected females. Embryos from infected females however survive to sustain the maternally inherited symbiont. Previously in Drosophila melanogaster flies, we demonstrated that CI modifies chromatin integrity in developing sperm to bestow the embryonic lethality...
March 28, 2024: PLoS Biology
https://read.qxmd.com/read/38527087/the-scale-of-zebrafish-pectoral-fin-buds-is-determined-by-intercellular-k-levels-and-consequent-ca2-mediated-signaling-via-retinoic-acid-regulation-of-rcan2-and-kcnk5b
#3
JOURNAL ARTICLE
Xiaowen Jiang, Kun Zhao, Yi Sun, Xinyue Song, Chao Yi, Tianlong Xiong, Sen Wang, Yi Yu, Xiduo Chen, Run Liu, Xin Yan, Christopher L Antos
K+ channels regulate morphogens to scale adult fins, but little is known about what regulates the channels and how they control morphogen expression. Using the zebrafish pectoral fin bud as a model for early vertebrate fin/limb development, we found that K+ channels also scale this anatomical structure, and we determined how one K+-leak channel, Kcnk5b, integrates into its developmental program. From FLIM measurements of a FRET-based K+ sensor, we observed coordinated decreases in intracellular K+ levels during bud growth, and overexpression of K+-leak channels in vivo coordinately increased bud proportions...
March 25, 2024: PLoS Biology
https://read.qxmd.com/read/38517908/one-size-does-not-fit-all-lysosomes-exist-in-biochemically-and-functionally-distinct-states
#4
JOURNAL ARTICLE
Claudio Bussi, Maximiliano G Gutierrez
Single-organelle resolution approaches have the potential to advance our knowledge of the heterogeneity of lysosome function. Challenging population-based models, we propose a "lysosome states" concept that links single lysosomes to function.
March 22, 2024: PLoS Biology
https://read.qxmd.com/read/38512963/communication-between-the-stem-cell-niche-and-an-adjacent-differentiation-niche-through-mirna-and-egfr-signaling-orchestrates-exit-from-the-stem-cell-state-in-the-drosophila-ovary
#5
JOURNAL ARTICLE
Jiani Chen, Chaoqun Li, Yifeng Sheng, Junwei Zhang, Lan Pang, Zhi Dong, Zhiwei Wu, Yueqi Lu, Zhiguo Liu, Qichao Zhang, Xueying Guan, Xuexin Chen, Jianhua Huang
The signaling environment, or niche, often governs the initial difference in behavior of an adult stem cell and a derivative that initiates a path towards differentiation. The transition between an instructive stem cell niche and differentiation niche must generally have single-cell resolution, suggesting that multiple mechanisms might be necessary to sharpen the transition. Here, we examined the Drosophila ovary and found that Cap cells, which are key constituents of the germline stem cell (GSC) niche, express a conserved microRNA (miR-124)...
March 21, 2024: PLoS Biology
https://read.qxmd.com/read/38489394/the-evolutionary-safety-of-mutagenic-drugs-should-be-assessed-before-drug-approval
#6
JOURNAL ARTICLE
Gabriela Lobinska, Vyacheslav Tretyachenko, Orna Dahan, Martin A Nowak, Yitzhak Pilpel
Some drugs increase the mutation rate of their target pathogen, a potentially concerning mechanism as the pathogen might evolve faster toward an undesired phenotype. We suggest a four-step assessment of evolutionary safety for the approval of such treatments.
March 15, 2024: PLoS Biology
https://read.qxmd.com/read/38483978/engineering-of-cas12a-nuclease-variants-with-enhanced-genome-editing-specificity
#7
JOURNAL ARTICLE
Peng Chen, Jin Zhou, Huan Liu, Erchi Zhou, Boxiao He, Yankang Wu, Hongjian Wang, Zaiqiao Sun, Chonil Paek, Jun Lei, Yongshun Chen, Xinghua Zhang, Lei Yin
The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a system is a powerful tool in gene editing; however, crRNA-DNA mismatches might induce unwanted cleavage events, especially at the distal end of the PAM. To minimize this limitation, we engineered a hyper fidelity AsCas12a variant carrying the mutations S186A/R301A/T315A/Q1014A/K414A (termed HyperFi-As) by modifying amino acid residues interacting with the target DNA and crRNA strand. HyperFi-As retains on-target activities comparable to wild-type AsCas12a (AsCas12aWT) in human cells...
March 14, 2024: PLoS Biology
https://read.qxmd.com/read/38478588/polyphosphate-kinase-regulates-lps-structure-and-polymyxin-resistance-during-starvation-in-e-coli
#8
JOURNAL ARTICLE
Kanchi Baijal, Iryna Abramchuk, Carmen M Herrera, Thien-Fah Mah, M Stephen Trent, Mathieu Lavallée-Adam, Michael Downey
Polyphosphates (polyP) are chains of inorganic phosphates that can reach over 1,000 residues in length. In Escherichia coli, polyP is produced by the polyP kinase (PPK) and is thought to play a protective role during the response to cellular stress. However, the molecular pathways impacted by PPK activity and polyP accumulation remain poorly characterized. In this work, we used label-free mass spectrometry to study the response of bacteria that cannot produce polyP (Δppk) during starvation to identify novel pathways regulated by PPK...
March 13, 2024: PLoS Biology
https://read.qxmd.com/read/38478582/neuronal-ageing-is-promoted-by-the-decay-of-the-microtubule-cytoskeleton
#9
JOURNAL ARTICLE
Pilar Okenve-Ramos, Rory Gosling, Monika Chojnowska-Monga, Kriti Gupta, Samuel Shields, Haifa Alhadyian, Ceryce Collie, Emilia Gregory, Natalia Sanchez-Soriano
Natural ageing is accompanied by a decline in motor, sensory, and cognitive functions, all impacting quality of life. Ageing is also the predominant risk factor for many neurodegenerative diseases, including Parkinson's disease and Alzheimer's disease. We need to therefore gain a better understanding of the cellular and physiological processes underlying age-related neuronal decay. However, gaining this understanding is a slow process due to the large amount of time required to age mammalian or vertebrate animal models...
March 13, 2024: PLoS Biology
https://read.qxmd.com/read/38478577/specification-of-distinct-cell-types-in-a-sensory-adhesive-organ-important-for-metamorphosis-in-tunicate-larvae
#10
JOURNAL ARTICLE
Christopher J Johnson, Florian Razy-Krajka, Fan Zeng, Katarzyna M Piekarz, Shweta Biliya, Ute Rothbächer, Alberto Stolfi
The papillae of tunicate larvae contribute sensory, adhesive, and metamorphosis-regulating functions that are crucial for the biphasic lifestyle of these marine, non-vertebrate chordates. We have identified additional molecular markers for at least 5 distinct cell types in the papillae of the model tunicate Ciona, allowing us to further study the development of these organs. Using tissue-specific CRISPR/Cas9-mediated mutagenesis and other molecular perturbations, we reveal the roles of key transcription factors and signaling pathways that are important for patterning the papilla territory into a highly organized array of different cell types and shapes...
March 13, 2024: PLoS Biology
https://read.qxmd.com/read/38470934/noncanonical-and-reversible-cysteine-ubiquitination-prevents-the-overubiquitination-of-pex5-at-the-peroxisomal-membrane
#11
JOURNAL ARTICLE
Tânia Francisco, Ana G Pedrosa, Tony A Rodrigues, Tarad Abalkhail, Hongli Li, Maria J Ferreira, Gerbrand J van der Heden van Noort, Marc Fransen, Ewald H Hettema, Jorge E Azevedo
PEX5, the peroxisomal protein shuttling receptor, binds newly synthesized proteins in the cytosol and transports them to the organelle. During its stay at the peroxisomal protein translocon, PEX5 is monoubiquitinated at its cysteine 11 residue, a mandatory modification for its subsequent ATP-dependent extraction back into the cytosol. The reason why a cysteine and not a lysine residue is the ubiquitin acceptor is unknown. Using an established rat liver-based cell-free in vitro system, we found that, in contrast to wild-type PEX5, a PEX5 protein possessing a lysine at position 11 is polyubiquitinated at the peroxisomal membrane, a modification that negatively interferes with the extraction process...
March 12, 2024: PLoS Biology
https://read.qxmd.com/read/38466773/intact-a-nondisruptive-internal-tagging-strategy-to-study-the-organization-and-function-of-actin-isoforms
#12
JOURNAL ARTICLE
Maxime C van Zwam, Anubhav Dhar, Willem Bosman, Wendy van Straaten, Suzanne Weijers, Emiel Seta, Ben Joosten, Jeffrey van Haren, Saravanan Palani, Koen van den Dries
Mammals have 6 highly conserved actin isoforms with nonredundant biological functions. The molecular basis of isoform specificity, however, remains elusive due to a lack of tools. Here, we describe the development of IntAct, an internal tagging strategy to study actin isoforms in fixed and living cells. We identified a residue pair in β-actin that permits tag integration and used knock-in cell lines to demonstrate that IntAct β-actin expression and filament incorporation is indistinguishable from wild type...
March 11, 2024: PLoS Biology
https://read.qxmd.com/read/38466754/an-ancient-bacterial-zinc-acquisition-system-identified-from-a-cyanobacterial-exoproteome
#13
JOURNAL ARTICLE
Cristina Sarasa-Buisan, Jesús A G Ochoa de Alda, Cristina Velázquez-Suárez, Miguel Ángel Rubio, Guadalupe Gómez-Baena, María F Fillat, Ignacio Luque
Bacteria have developed fine-tuned responses to cope with potential zinc limitation. The Zur protein is a key player in coordinating this response in most species. Comparative proteomics conducted on the cyanobacterium Anabaena highlighted the more abundant proteins in a zur mutant compared to the wild type. Experimental evidence showed that the exoprotein ZepA mediates zinc uptake. Genomic context of the zepA gene and protein structure prediction provided additional insights on the regulation and putative function of ZepA homologs...
March 11, 2024: PLoS Biology
https://read.qxmd.com/read/38466732/proteasome-inhibition-triggers-tissue-specific-immune-responses-against-different-pathogens-in-c-elegans
#14
JOURNAL ARTICLE
Manish Grover, Spencer S Gang, Emily R Troemel, Michalis Barkoulas
Protein quality control pathways play important roles in resistance against pathogen infection. For example, the conserved transcription factor SKN-1/NRF up-regulates proteostasis capacity after blockade of the proteasome and also promotes resistance against bacterial infection in the nematode Caenorhabditis elegans. SKN-1/NRF has 3 isoforms, and the SKN-1A/NRF1 isoform, in particular, regulates proteasomal gene expression upon proteasome dysfunction as part of a conserved bounce-back response. We report here that, in contrast to the previously reported role of SKN-1 in promoting resistance against bacterial infection, loss-of-function mutants in skn-1a and its activating enzymes ddi-1 and png-1 show constitutive expression of immune response programs against natural eukaryotic pathogens of C...
March 11, 2024: PLoS Biology
https://read.qxmd.com/read/38466718/widespread-prevalence-of-a-methylation-dependent-switch-to-activate-an-essential-dna-damage-response-in-bacteria
#15
JOURNAL ARTICLE
Aditya Kamat, Ngat T Tran, Mohak Sharda, Neha Sontakke, Tung B K Le, Anjana Badrinarayanan
DNA methylation plays central roles in diverse cellular processes, ranging from error-correction during replication to regulation of bacterial defense mechanisms. Nevertheless, certain aberrant methylation modifications can have lethal consequences. The mechanisms by which bacteria detect and respond to such damage remain incompletely understood. Here, we discover a highly conserved but previously uncharacterized transcription factor (Cada2), which orchestrates a methylation-dependent adaptive response in Caulobacter...
March 11, 2024: PLoS Biology
https://read.qxmd.com/read/38466713/attention-to-audiovisual-speech-shapes-neural-processing-through-feedback-feedforward-loops-between-different-nodes-of-the-speech-network
#16
JOURNAL ARTICLE
Patrik Wikman, Viljami Salmela, Eetu Sjöblom, Miika Leminen, Matti Laine, Kimmo Alho
Selective attention-related top-down modulation plays a significant role in separating relevant speech from irrelevant background speech when vocal attributes separating concurrent speakers are small and continuously evolving. Electrophysiological studies have shown that such top-down modulation enhances neural tracking of attended speech. Yet, the specific cortical regions involved remain unclear due to the limited spatial resolution of most electrophysiological techniques. To overcome such limitations, we collected both electroencephalography (EEG) (high temporal resolution) and functional magnetic resonance imaging (fMRI) (high spatial resolution), while human participants selectively attended to speakers in audiovisual scenes containing overlapping cocktail party speech...
March 11, 2024: PLoS Biology
https://read.qxmd.com/read/38427710/structural-and-functional-characterization-of-afsr-an-sarp-family-transcriptional-activator-of-antibiotic-biosynthesis-in-streptomyces
#17
JOURNAL ARTICLE
Yiqun Wang, Xu Yang, Feng Yu, Zixin Deng, Shuangjun Lin, Jianting Zheng
Streptomyces antibiotic regulatory proteins (SARPs) are widely distributed activators of antibiotic biosynthesis. Streptomyces coelicolor AfsR is an SARP regulator with an additional nucleotide-binding oligomerization domain (NOD) and a tetratricopeptide repeat (TPR) domain. Here, we present cryo-electron microscopy (cryo-EM) structures and in vitro assays to demonstrate how the SARP domain activates transcription and how it is modulated by NOD and TPR domains. The structures of transcription initiation complexes (TICs) show that the SARP domain forms a side-by-side dimer to simultaneously engage the afs box overlapping the -35 element and the σHrdB region 4 (R4), resembling a sigma adaptation mechanism...
March 1, 2024: PLoS Biology
https://read.qxmd.com/read/38427708/value-related-learning-in-the-olfactory-bulb-occurs-through-pathway-dependent-perisomatic-inhibition-of-mitral-cells
#18
JOURNAL ARTICLE
Sander Lindeman, Xiaochen Fu, Janine Kristin Reinert, Izumi Fukunaga
Associating values to environmental cues is a critical aspect of learning from experiences, allowing animals to predict and maximise future rewards. Value-related signals in the brain were once considered a property of higher sensory regions, but their wide distribution across many brain regions is increasingly recognised. Here, we investigate how reward-related signals begin to be incorporated, mechanistically, at the earliest stage of olfactory processing, namely, in the olfactory bulb. In head-fixed mice performing Go/No-Go discrimination of closely related olfactory mixtures, rewarded odours evoke widespread inhibition in one class of output neurons, that is, in mitral cells but not tufted cells...
March 1, 2024: PLoS Biology
https://read.qxmd.com/read/38427703/an-adapted-ms2-mcp-system-to-visualize-endogenous-cytoplasmic-mrna-with-live-imaging-in-caenorhabditis-elegans
#19
JOURNAL ARTICLE
Cristina Tocchini, Susan E Mango
Live imaging of RNA molecules constitutes an invaluable means to track the dynamics of mRNAs, but live imaging in Caenorhabditis elegans has been difficult to achieve. Endogenous transcripts have been observed in nuclei, but endogenous mRNAs have not been detected in the cytoplasm, and functional mRNAs have not been generated. Here, we have adapted live imaging methods to visualize mRNA in embryonic cells. We have tagged endogenous transcripts with MS2 hairpins in the 3' untranslated region (UTR) and visualized them after adjusting MS2 Coat Protein (MCP) expression...
March 1, 2024: PLoS Biology
https://read.qxmd.com/read/38422172/cd38-promotes-hematopoietic-stem-cell-dormancy
#20
JOURNAL ARTICLE
Liliia Ibneeva, Sumeet Pal Singh, Anupam Sinha, Sema Elif Eski, Rebekka Wehner, Luise Rupp, Iryna Kovtun, Juan Alberto Pérez-Valencia, Alexander Gerbaulet, Susanne Reinhardt, Manja Wobus, Malte von Bonin, Jaime Sancho, Frances Lund, Andreas Dahl, Marc Schmitz, Martin Bornhäuser, Triantafyllos Chavakis, Ben Wielockx, Tatyana Grinenko
A subpopulation of deeply quiescent, so-called dormant hematopoietic stem cells (dHSCs) resides at the top of the hematopoietic hierarchy and serves as a reserve pool for HSCs. The state of dormancy protects the HSC pool from exhaustion throughout life; however, excessive dormancy may prevent an efficient response to hematological stresses. Despite the significance of dHSCs, the mechanisms maintaining their dormancy remain elusive. Here, we identify CD38 as a novel and broadly applicable surface marker for the enrichment of murine dHSCs...
February 29, 2024: PLoS Biology
journal
journal
40488
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.