journal
MENU ▼
Read by QxMD icon Read
search

PLoS Biology

journal
https://www.readbyqxmd.com/read/29049289/a-molecular-atlas-of-the-developing-ectoderm-defines-neural-neural-crest-placode-and-nonneural-progenitor-identity-in-vertebrates
#1
Jean-Louis Plouhinec, Sofía Medina-Ruiz, Caroline Borday, Elsa Bernard, Jean-Philippe Vert, Michael B Eisen, Richard M Harland, Anne H Monsoro-Burq
During vertebrate neurulation, the embryonic ectoderm is patterned into lineage progenitors for neural plate, neural crest, placodes and epidermis. Here, we use Xenopus laevis embryos to analyze the spatial and temporal transcriptome of distinct ectodermal domains in the course of neurulation, during the establishment of cell lineages. In order to define the transcriptome of small groups of cells from a single germ layer and to retain spatial information, dorsal and ventral ectoderm was subdivided along the anterior-posterior and medial-lateral axes by microdissections...
October 19, 2017: PLoS Biology
https://www.readbyqxmd.com/read/29036170/quantifying-the-effects-of-temperature-on-mosquito-and-parasite-traits-that-determine-the-transmission-potential-of-human-malaria
#2
Lillian L M Shapiro, Shelley A Whitehead, Matthew B Thomas
Malaria transmission is known to be strongly impacted by temperature. The current understanding of how temperature affects mosquito and parasite life history traits derives from a limited number of empirical studies. These studies, some dating back to the early part of last century, are often poorly controlled, have limited replication, explore a narrow range of temperatures, and use a mixture of parasite and mosquito species. Here, we use a single pairing of the Asian mosquito vector, An. stephensi and the human malaria parasite, P...
October 16, 2017: PLoS Biology
https://www.readbyqxmd.com/read/29049280/ethoscopes-an-open-platform-for-high-throughput-ethomics
#3
Quentin Geissmann, Luis Garcia Rodriguez, Esteban J Beckwith, Alice S French, Arian R Jamasb, Giorgio F Gilestro
Here, we present the use of ethoscopes, which are machines for high-throughput analysis of behavior in Drosophila and other animals. Ethoscopes provide a software and hardware solution that is reproducible and easily scalable. They perform, in real-time, tracking and profiling of behavior by using a supervised machine learning algorithm, are able to deliver behaviorally triggered stimuli to flies in a feedback-loop mode, and are highly customizable and open source. Ethoscopes can be built easily by using 3D printing technology and rely on Raspberry Pi microcomputers and Arduino boards to provide affordable and flexible hardware...
October 2017: PLoS Biology
https://www.readbyqxmd.com/read/29045398/nonlatching-positive-feedback-enables-robust-bimodality-by-decoupling-expression-noise-from-the-mean
#4
Brandon S Razooky, Youfang Cao, Maike M K Hansen, Alan S Perelson, Michael L Simpson, Leor S Weinberger
Fundamental to biological decision-making is the ability to generate bimodal expression patterns where 2 alternate expression states simultaneously exist. Here, we use a combination of single-cell analysis and mathematical modeling to examine the sources of bimodality in the transcriptional program controlling HIV's fate decision between active replication and viral latency. We find that the HIV transactivator of transcription (Tat) protein manipulates the intrinsic toggling of HIV's promoter, the long terminal repeat (LTR), to generate bimodal ON-OFF expression and that transcriptional positive feedback from Tat shifts and expands the regime of LTR bimodality...
October 2017: PLoS Biology
https://www.readbyqxmd.com/read/29023486/the-sea-cucumber-genome-provides-insights-into-morphological-evolution-and-visceral-regeneration
#5
Xiaojun Zhang, Lina Sun, Jianbo Yuan, Yamin Sun, Yi Gao, Libin Zhang, Shihao Li, Hui Dai, Jean-François Hamel, Chengzhang Liu, Yang Yu, Shilin Liu, Wenchao Lin, Kaimin Guo, Songjun Jin, Peng Xu, Kenneth B Storey, Pin Huan, Tao Zhang, Yi Zhou, Jiquan Zhang, Chenggang Lin, Xiaoni Li, Lili Xing, Da Huo, Mingzhe Sun, Lei Wang, Annie Mercier, Fuhua Li, Hongsheng Yang, Jianhai Xiang
Apart from sharing common ancestry with chordates, sea cucumbers exhibit a unique morphology and exceptional regenerative capacity. Here we present the complete genome sequence of an economically important sea cucumber, A. japonicus, generated using Illumina and PacBio platforms, to achieve an assembly of approximately 805 Mb (contig N50 of 190 Kb and scaffold N50 of 486 Kb), with 30,350 protein-coding genes and high continuity. We used this resource to explore key genetic mechanisms behind the unique biological characters of sea cucumbers...
October 2017: PLoS Biology
https://www.readbyqxmd.com/read/28976974/the-sex-of-specific-neurons-controls-female-body-growth-in-drosophila
#6
Annick Sawala, Alex P Gould
Sexual dimorphisms in body size are widespread throughout the animal kingdom but their underlying mechanisms are not well characterized. Most models for how sex chromosome genes specify size dimorphism have emphasized the importance of gonadal hormones and cell-autonomous influences in mammals versus strictly cell-autonomous mechanisms in Drosophila melanogaster. Here, we use tissue-specific genetics to investigate how sexual size dimorphism (SSD) is established in Drosophila. We find that the larger body size characteristic of Drosophila females is established very early in larval development via an increase in the growth rate per unit of body mass...
October 2017: PLoS Biology
https://www.readbyqxmd.com/read/28968388/a-critical-evaluation-of-the-algorithm-behind-the-relative-citation-ratio-rcr
#7
A Cecile J W Janssens, Michael Goodman, Kimberly R Powell, Marta Gwinn
No abstract text is available yet for this article.
October 2017: PLoS Biology
https://www.readbyqxmd.com/read/28968381/additional-support-for-rcr-a-validated-article-level-measure-of-scientific-influence
#8
B Ian Hutchins, Travis A Hoppe, Rebecca A Meseroll, James M Anderson, George M Santangelo
No abstract text is available yet for this article.
October 2017: PLoS Biology
https://www.readbyqxmd.com/read/28961236/transcriptome-analysis-of-hypoxic-cancer-cells-uncovers-intron-retention-in-eif2b5-as-a-mechanism-to-inhibit-translation
#9
Lauren K Brady, Hejia Wang, Caleb M Radens, Yue Bi, Milan Radovich, Amit Maity, Cristina Ivan, Mircea Ivan, Yoseph Barash, Constantinos Koumenis
Cells adjust to hypoxic stress within the tumor microenvironment by downregulating energy-consuming processes including translation. To delineate mechanisms of cellular adaptation to hypoxia, we performed RNA-Seq of normoxic and hypoxic head and neck cancer cells. These data revealed a significant down regulation of genes known to regulate RNA processing and splicing. Exon-level analyses classified > 1,000 mRNAs as alternatively spliced under hypoxia and uncovered a unique retained intron (RI) in the master regulator of translation initiation, EIF2B5...
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28957312/the-experimental-design-assistant
#10
Nathalie Percie du Sert, Ian Bamsey, Simon T Bate, Manuel Berdoy, Robin A Clark, Innes Cuthill, Derek Fry, Natasha A Karp, Malcolm Macleod, Lawrence Moon, S Clare Stanford, Brian Lings
Addressing the common problems that researchers encounter when designing and analysing animal experiments will improve the reliability of in vivo research. In this article, the Experimental Design Assistant (EDA) is introduced. The EDA is a web-based tool that guides the in vivo researcher through the experimental design and analysis process, providing automated feedback on the proposed design and generating a graphical summary that aids communication with colleagues, funders, regulatory authorities, and the wider scientific community...
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28953896/dynamic-patterning-by-the-drosophila-pair-rule-network-reconciles-long-germ-and-short-germ-segmentation
#11
Erik Clark
Drosophila segmentation is a well-established paradigm for developmental pattern formation. However, the later stages of segment patterning, regulated by the "pair-rule" genes, are still not well understood at the system level. Building on established genetic interactions, I construct a logical model of the Drosophila pair-rule system that takes into account the demonstrated stage-specific architecture of the pair-rule gene network. Simulation of this model can accurately recapitulate the observed spatiotemporal expression of the pair-rule genes, but only when the system is provided with dynamic "gap" inputs...
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28938018/genome-wide-identification-of-bacterial-plant-colonization-genes
#12
Benjamin J Cole, Meghan E Feltcher, Robert J Waters, Kelly M Wetmore, Tatiana S Mucyn, Elizabeth M Ryan, Gaoyan Wang, Sabah Ul-Hasan, Meredith McDonald, Yasuo Yoshikuni, Rex R Malmstrom, Adam M Deutschbauer, Jeffery L Dangl, Axel Visel
Diverse soil-resident bacteria can contribute to plant growth and health, but the molecular mechanisms enabling them to effectively colonize their plant hosts remain poorly understood. We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomonas simiae, a model root-colonizing bacterium, to establish a genome-wide map of bacterial genes required for colonization of the Arabidopsis thaliana root system. We identified 115 genes (2% of all P. simiae genes) with functions that are required for maximal competitive colonization of the root system...
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28937978/a-new-discrete-dynamic-model-of-aba-induced-stomatal-closure-predicts-key-feedback-loops
#13
Réka Albert, Biswa R Acharya, Byeong Wook Jeon, Jorge G T Zañudo, Mengmeng Zhu, Karim Osman, Sarah M Assmann
Stomata, microscopic pores in leaf surfaces through which water loss and carbon dioxide uptake occur, are closed in response to drought by the phytohormone abscisic acid (ABA). This process is vital for drought tolerance and has been the topic of extensive experimental investigation in the last decades. Although a core signaling chain has been elucidated consisting of ABA binding to receptors, which alleviates negative regulation by protein phosphatases 2C (PP2Cs) of the protein kinase OPEN STOMATA 1 (OST1) and ultimately results in activation of anion channels, osmotic water loss, and stomatal closure, over 70 additional components have been identified, yet their relationships with each other and the core components are poorly elucidated...
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28934203/development-of-rat-female-genital-cortex-and-control-of-female-puberty-by-sexual-touch
#14
Constanze Lenschow, Johanna Sigl-Glöckner, Michael Brecht
Rat somatosensory cortex contains a large sexually monomorphic genital representation. Genital cortex undergoes an unusual 2-fold expansion during puberty. Here, we investigate genital cortex development and female rat sexual maturation. Ovariectomies and estradiol injections suggested sex hormones cause the pubertal genital cortex expansion but not its maintenance at adult size. Genital cortex expanded by thalamic afferents invading surrounding dysgranular cortex. Genital touch was a dominant factor driving female sexual maturation...
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28926570/what-do-we-talk-about-when-we-talk-about-rhythm
#15
Jonas Obleser, Molly J Henry, Peter Lakatos
No abstract text is available yet for this article.
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28926563/dance-to-the-rhythm-cautiously-isolating-unique-indicators-of-oscillatory-entrainment
#16
Assaf Breska, Leon Y Deouell
No abstract text is available yet for this article.
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28922352/60-years-ago-francis-crick-changed-the-logic-of-biology
#17
Matthew Cobb
In September 1957, Francis Crick gave a lecture in which he outlined key ideas about gene function, in particular what he called the central dogma. These ideas still frame how we understand life. This essay explores the concepts he developed in this influential lecture, including his prediction that we would study evolution by comparing sequences.
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28902870/reduced%C3%A2-insulin-signaling-maintains-electrical-transmission-in-a-neural-circuit-in-aging-flies
#18
Hrvoje Augustin, Kieran McGourty, Marcus J Allen, Sirisha Kudumala Madem, Jennifer Adcott, Fiona Kerr, Chi Tung Wong, Alec Vincent, Tanja Godenschwege, Emmanuel Boucrot, Linda Partridge
Lowered insulin/insulin-like growth factor (IGF) signaling (IIS) can extend healthy lifespan in worms, flies, and mice, but it can also have adverse effects (the "insulin paradox"). Chronic, moderately lowered IIS rescues age-related decline in neurotransmission through the Drosophila giant fiber system (GFS), a simple escape response neuronal circuit, by increasing targeting of the gap junctional protein innexin shaking-B to gap junctions (GJs). Endosomal recycling of GJs was also stimulated in cultured human cells when IIS was reduced...
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28898248/place-field-assembly-distribution-encodes-preferred-locations
#19
Omar Mamad, Lars Stumpp, Harold M McNamara, Charu Ramakrishnan, Karl Deisseroth, Richard B Reilly, Marian Tsanov
The hippocampus is the main locus of episodic memory formation and the neurons there encode the spatial map of the environment. Hippocampal place cells represent location, but their role in the learning of preferential location remains unclear. The hippocampus may encode locations independently from the stimuli and events that are associated with these locations. We have discovered a unique population code for the experience-dependent value of the context. The degree of reward-driven navigation preference highly correlates with the spatial distribution of the place fields recorded in the CA1 region of the hippocampus...
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28892507/extreme-genome-diversity-in-the-hyper-prevalent-parasitic-eukaryote-blastocystis
#20
COMPARATIVE STUDY
Eleni Gentekaki, Bruce A Curtis, Courtney W Stairs, Vladimír Klimeš, Marek Eliáš, Dayana E Salas-Leiva, Emily K Herman, Laura Eme, Maria C Arias, Bernard Henrissat, Frédérique Hilliou, Mary J Klute, Hiroshi Suga, Shehre-Banoo Malik, Arthur W Pightling, Martin Kolisko, Richard A Rachubinski, Alexander Schlacht, Darren M Soanes, Anastasios D Tsaousis, John M Archibald, Steven G Ball, Joel B Dacks, C Graham Clark, Mark van der Giezen, Andrew J Roger
Blastocystis is the most prevalent eukaryotic microbe colonizing the human gut, infecting approximately 1 billion individuals worldwide. Although Blastocystis has been linked to intestinal disorders, its pathogenicity remains controversial because most carriers are asymptomatic. Here, the genome sequence of Blastocystis subtype (ST) 1 is presented and compared to previously published sequences for ST4 and ST7. Despite a conserved core of genes, there is unexpected diversity between these STs in terms of their genome sizes, guanine-cytosine (GC) content, intron numbers, and gene content...
September 2017: PLoS Biology
journal
journal
40488
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"