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Journal of Oleo Science

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https://www.readbyqxmd.com/read/28626144/glass-bead-based-genetic-transformation-an-efficient-method-for-transformation-of-thraustochytrid-microorganisms
#1
Takumi Adachi, Takehiko Sahara, Hidetoshi Okuyama, Naoki Morita
Here, we describe a new method for genetic transformation of thraustochytrids, well-known producers of polyunsaturated fatty acids (PUFAs) like docosahexaenoic acid, by combining mild glass (zirconia) bead treatment and electroporation. Because the cell wall is a barrier against transfer of exogenous DNA into cells, gentle vortexing of cells with glass beads was performed prior to electroporation for partial cell wall disruption. G418-resistant transformants of thraustochytrid cells (Aurantiochytrium limacinum strain SR21 and thraustochytrid strain 12B) were successfully obtained with good reproducibility...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626143/effect-of-conjugated-linoleic-acid-intake-on-endurance-exercise-performance-and-anti-fatigue-in-student-athletes
#2
Naoko Terasawa, Ken Okamoto, Kenta Nakada, Kazumi Masuda
Conjugated linoleic acids (CLA) intake has been reported to reduce body fat mass or increase lean body mass and to improve exercise outcome by modulating testosterone in humans. These reports have studied mostly overweight subjects; few were athletes. Therefore, in this study, the effect of CLA intake on endurance performance and anti-fatigue in student athletes was investigated. A double-blind, crossover study was conducted with 10 male student athletes. Each subject was administered with either CLA (net 0...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626142/surface-modification-of-pdms-and-plastics-with-zwitterionic-polymers
#3
Mutsuo Tanaka, Shigeru Kurosawa
Surface modification of PDMS, polycarbonate, and acrylic resin was examined using various methacryl polymers bearing sulfobetaine, phosphoryl choline, and oligoethylene glycol units. We have found that zwitterionic polymers are adsorbed on the PDMS surface treated with plasma. The surface of PDMS is stable to keep high hydrophilicity after a month of the modification. On the other hand, one of sulfobetaine polymers showed distinguished adsorption behavior in the case of polycarbonate surface treated with plasma...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626141/dietary-starfish-oil-prevents-hepatic-steatosis-and-hyperlipidemia-in-c57bl-6n-mice-fed-high-fat-diet
#4
Fumiaki Beppu, Haoqi Li, Kazuaki Yoshinaga, Toshiharu Nagai, Akihiko Yoshinda, Atsushi Kubo, Jota Kanda, Naohiro Gotoh
Starfish oil (SO) is characterized by functional lipids, including n-3 polyunsaturated fatty acid (both in the form of triacylglycerol and in the form of phospholipid), and carotenoids, which may exert beneficial effects on metabolic disorders in obesity-associated diseases. In the present study, the effect of SO on dysregulation of lipid metabolism was examined using C57BL/6N mice treated with high-fat (HF) diet. Mice were fed HF, HF with 2% SO, or HF with 5% SO diet for 8 weeks. Weight gain, blood glucose, serum and hepatic lipid contents, and hepatic fatty acid composition were measured...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626140/long-chain-polyunsaturated-fatty-acid-supplementation-improves-mood-in-elderly-japanese-men
#5
Hisanori Tokuda, Toshiaki Sueyasu, Hiroshi Kawashima, Hiroshi Shibata, Yoshihiko Koga
Although several studies have reported the effects of long-chain polyunsaturated fatty acid (LCPUFA) supplementation on the mood in healthy adults, the effects of LCPUFA on elderly individuals remain unclear. Thus, we hypothesized that LCPUFA supplementation improves mood in the elderly. To address this hypothesis, 115 elderly Japanese men aged 55-64 years were assigned and randomly allocated to the LCPUFA or placebo group. Participants received 4 weeks of supplementation with LCPUFAcontaining oil (docosahexaenoic acid (DHA) 300 mg/day, eicosapentaenoic acid (EPA) 100 mg/day, arachidonic acid (ARA) 120 mg/day) or a placebo oil...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626139/synthesis-of-ricinoleic-acid-estolides-by-the-esterification-of-ricinoleic-acids-using-functional-acid-ionic-liquids-as-catalysts
#6
Gaoshang Wang, Shangde Sun
Estolides of ricinoleic acid (RA) have been used as lubricants and pigment dispersant in many industries. In this paper, functional acid ionic liquids (ILs) were firstly used as catalysts to prepare RA estolides by the esterification of RAs in solvent-free system. Different ILs were used as catalysts for the esterification. Effect of reaction variables (IL amount, reaction temperature and reaction time) on the esterification were also investigated and optimized using response surface methodology (RSM). Among all tested ILs, [BSO3HMIM]TS showed the best performance for the esterification...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626138/evaluation-of-sorption-mechanism-of-pb-ii-andni-ii-onto-pea-pisum-sativum-peels
#7
Atta Ul Haq, Muhammad Saeed, Salma Anjum, Tanveer Hussain Bokhari, Muhammad Usman, Saiqa Tubbsum
The present study was carried out to know the sorption mechanism of Pb (II) and Ni (II) in aqueous solution using pea peels under the influence of sorbent dose, pH, temperature, initial metal ion concentration and contact time. SEM and FTIR were used for characterization of pea peels. The study showed that solution pH affects sorption process and the optimum pH for Pb (II) was 6.0 while for that of Ni (II) was 7.0. Pseudo-second order kinetic model was found to be the most suitable one to explain the kinetic data not only due to high value of R(2) (>0...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626137/novel-palm-fatty-acid-functionalized-magnetite-nanoparticles-for-magnetic-solid-phase-extraction-of-trace-polycyclic-aromatic-hydrocarbons-from-environmental-samples
#8
Siti Khalijah Mahmad Rozi, Hamid Rashidi Nodeh, Muhammad Afzal Kamboh, Ninie Suhana Abdul Manan, Sharifah Mohamad
A novel adsorbent, palm fatty acid coated magnetic Fe3O4 nanoparticles (MNP-FA) was successfully synthesized with immobilization of the palm fatty acid onto the surface of MNPs. The successful synthesis of MNP-FA was further confirmed by X-Ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and Energy dispersive X-Ray spectroscopy (EDX) analyses and water contact angle (WCA) measurement. This newly synthesized MNP-FA was applied as magnetic solid phase extraction (MSPE) adsorbent for the enrichment of polycyclic aromatic hydrocarbons (PAHs), namely fluoranthene (FLT), pyrene (Pyr), chrysene (Cry) and benzo(a)pyrene (BaP) from environmental samples prior to High Performance Liquid Chromatography- Diode Array Detector (HPLC-DAD) analysis...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626136/effect-of-hydroxyl-groups-on-solubilization-states-of-alkandiols-in-dtab-micelles-application-of-a-differential-conductivity-technique
#9
Hideo Kawamura, Yuuto Takeda, Takuro Tagawa, Hajime Katuura, Masaki Shiomi
The partition coefficients, Kx, of 1,2-alkanediols and α,ω-alkanediols between dodecyltrimethylammonium bromide (DTAB) micelles and water, and the change of the degree of counterion dissociation, dα/dXa(m), on DTAB micelles associated with solubilization of the alkanediols are determined by a differential conductivity technique. The standard Gibbs energy change of transfer per methylene group, ΔG(0)(CH2), is estimated from a dependence of logarithmic value of the Kx on the carbon number of alkyl chain in the alkanediols...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626135/adsorption-of-the-three-phase-emulsion-on-various-solid-surfaces
#10
Yasutaka Enomoto, Yoko Imai, Kazuo Tajima
The present study investigates the adsorption of the three-phase emulsion on various solid/water interfaces. Vesicles can be used as emulsifiers in the three-phase emulsions and act as an independent phase unlike the surfactant used in conventional emulsions; therefore, it is expected that the three-phase emulsion formed by the adhesion of vesicles to the oil/water interface will adsorb on various solid/water interfaces. The cationic three-phase emulsion was prepared to encourage emulsion adsorption on negatively charged solid substrates in water...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626134/synthesis-and-detection-by-hplc-of-3-oxohexadecanoyl-coa-for-the-study-of-peroxisomal-bifunctional-proteins
#11
Shirou Tsuchida, Ayumu Tenma, Naoya Hamaue, Tsuyoshi Murai, Teruki Yoshimura, Takashi Aoki, Takao Kurosawa
3-oxohexadecanoyl-CoA was synthesized for the study of D-bifunctional protein (EC 4. 2. 1. 107, EC 4. 2. 1. 119, EC 1. 1. 1. n12) and L-bifunctional protein (EC 4. 2. 1. 17, EC 5. 3. 3. 8, EC 1. 1. 1. 35). First, tetradecanal was subjected to the Reformatsky reaction with ethyl bromoacetate, and the product was then converted into ethyl 3-oxohexadecanoate. After acetalization of the 3-oxo ester with ethylene glycol, 3,3-ethlenedioxyhexadecanoic acid was obtained by alkaline hydrolysis. The acid was condensed with coenzyme A (CoA) by the mixed anhydride method, and the resulting CoA ester was deprotected with 4 M HCl to obtain 3-oxohexadecanoyl-CoA...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626133/simple-synthesis-hydrogenated-castor-oil-fatty-amide-wax-and-its-coating-characterization
#12
Xiuzhu Yu, Ning Wang, Rui Zhang, Zhong Zhao
A simple method for incorporating amine groups in hydrogenated castor oil (HCO) to produce wax for beeswax or carnauba wax substitution in packaging and coating was developed. From the conversion rate of the products, HCO was reacted with ethanolamine at 150°C for 5 h, and the molar ratio of HCO and ethanolamine was 1:4. The hardness of the final product was seven times higher than that of beeswax, the cohesiveness of the final product was 1.3 times higher than that of beeswax and approximately one half of that of carnauba wax, and the melting point of the final product is 98°C...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28626132/performance-of-palm-based-c16-18-methyl-ester-sulphonate-mes-in-liquid-detergent-formulation
#13
Zulina Abd Maurad, Zainab Idris, Razmah Ghazali
Liquid detergents are more convenient than powdered detergents as they dissolve readily in water, generate less dust and dosing is easy. However, the stability of liquid detergents is an issue of concern. Therefore, the objective of this research is to study the formulation requirement to produce heavy-duty liquid detergents based on palm-based methyl esters sulphonate (MES) with desirable properties and performance. MES is produced from renewable and sustainable feedstock suitable to replace the conventional fossil-based surfactant, linear alkyl benzene sulphonates (LAS)...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28515386/autoxidation-of-fish-oil-blended-with-rice-bran-oil
#14
Shigeo Nakajima, Marie Takai, Chieko Hayashi, Takuo Tsuno, Yasushi Endo
Effects of rice bran oil on the oxidative and flavor stability of fish oil were investigated by the gas liquid chromatography-head space method. When fish oil blending with different ratio of rice bran oils was oxidized at room temperature in the dark, volatile compounds produced during autoxidation was measured by gas liquid chromatography. The amounts of volatile compounds were decreased with increased the ratio of blended rice bran oil as well as peroxide value. The level of propanal and acrolein which gave unpleasant flavor was also decreased with increased the ratio of blended rice bran oil...
May 17, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28515385/supercritical-co2-extraction-of-rice-bran-oil-the-technology-manufacture-and-applications
#15
Phumon Sookwong, Sugunya Mahatheeranont
Rice bran is a good source of nutrients that have large amounts of phytochemicals and antioxidants. Conventional rice bran oil production requires many processes that may deteriorate and degrade these valuable substances. Supercritical CO2 extraction is a green alternative method for producing rice bran oil. This work reviews production of rice bran oil by supercritical carbon dioxide (SC-CO2) extraction. In addition, the usefulness and advantages of SC-CO2 extracted rice bran oil for edible oil and health purpose is also described...
May 17, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28515384/physicochemical-and-antioxidant-properties-of-rice-bran-oils-produced-from-colored-rice-using-different-extraction-methods
#16
Sukanya Mingyai, Aikkarach Kettawan, Khongsak Srikaeo, Riantong Singanusong
This study investigated the physicochemical and antioxidant properties of rice bran oil (RBO) produced from the bran of three rice varities; Khao Dawk Mali 105 (white rice), Red Jasmine rice (red rice) and Hom-nin rice (black rice) using three extraction methods including cold-press extraction (CPE), solvent extraction (SE) and supercritical CO2 extraction (SC-CO2). Yields, color, acid value (AV), free fatty acid (FFA), peroxide value (PV), iodine value (IV), total phenolic compound (TPC), γ-oryzanol, α-tocopherol and fatty acid profile were analyzed...
May 17, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28515383/a-new-agonist-for-peroxisome-proliferationactivated-receptor-%C3%AE-ppar%C3%AE-fraglide-1-from-zhenjiang-fragrant-vinegar-screening-and-characterization-based-on-cell-culture-experiments
#17
Yoshio Tsujino
Zhenjiang fragrant vinegar (Kozu) is a black rice vinegar that has been used as a traditional Chinese medicine and has various health benefits, including anti-obesity effects. In the present study, using luciferase reporter assays of PPARγ promoter activity, a novel ingredient from 8-year-old Kozu, 5-hydroxy-4-phenyl-butenolide, was isolated. The newly found agonist was named as "Fraglide-1". Moreover, in subsequent experiments, it was confirmed that fraglide-1 was an PPARγ agonist and it could increase expression level of the uncoupling protein (UCP)-1...
May 17, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28515382/screening-evaluation-of-the-interaction-of-linear-chain-or-branched-chain-peptides-with-multilamellar-vesicle-using-confocal-laser-microscopy
#18
Junko Kuwahara, Tetsuya Marume, Hajime Mita
In the present research, we prepared an acidic liposome as a cell model and used confocal laser microscopy to evaluate its interaction with oligopeptides that had high membrane permeability or affinity. The results showed that, for short peptides of about ten residues, the positive charge peptides interacted with the acidic liposome strongly. For peptides that interacted with the liposome, no difference was found between linear-chain and branched-chain peptides due to their structure.
May 17, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28515381/preliminary-evaluation-of-glyceric-acid-producing-ability-of-acidomonas-methanolica-nbrc104435-from-glycerol-containing-methanol
#19
Shun Sato, Dai Kitamoto, Hiroshi Habe
Some acetic acid bacteria produce large amounts of glyceric acid (GA) from glycerol in culture broth. However, methanol, which is a major contaminant of raw glycerol derived from the biodiesel fuel industry, sharply decreases cell growth and GA production [AMB Express, 3, 20, 2013]. Thus, we evaluated the methylotrophic acetic acid bacterium Acidomonas methanolica NBRC104435 for its ability to produce GA from glycerol containing methanol. This strain accumulated GA in its culture broth when 1-3 wt% glycerol was available as a carbon source...
May 17, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28515380/highly-selective-and-considerable-subcritical-butane-extraction-to-separate-abamectin-in-green-tea
#20
Yating Zhang, Lingbiao Gu, Fei Wang, Lingjun Kong, Huili Pang, Guangyong Qin
We specially carried out the subcritical butane extraction to separate abamectin from tea leaves. Four parameters, such as extraction temperature, extraction time, number of extraction cycles, and solid-liquid ratio were studied and optimized through the response surface methodology with design matrix developed by Box-Behnken. Seventeen experiments with three various factors and three variable levels were employed to investigate the effect of these parameters on the extraction of abamectin. Besides, catechins, theanine, caffeine, and aroma components were determined by both high-performance liquid chromatography and gas chromatography-mass spectrometry to evaluate the tea quality before and after the extraction...
May 17, 2017: Journal of Oleo Science
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