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Genetics and Molecular Research: GMR

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https://www.readbyqxmd.com/read/28437560/phylogeography-and-population-structure-analysis-reveals-diversity-by-mutations-in-lasiodiplodia-theobromae-with-distinct-sources-of-selection
#1
P H D Santos, B M Carvalho, K P Aguiar, F A S Aredes, T P S Poltronieri, J M S Vivas, V Mussi Dias, G A Bezerra, D B Pinho, M G Pereira, S F Silveira
Lasiodiplodia theobromae is a plant pathogen with a worldwide distribution, with low host specificity, causing stem cankers, dieback diseases, and fruit rot in several species of plants. In coconut, this pathogen is reported as the etiological agent of "coconut leaf blight" (CLB) disease, causing several losses in fruit production. The CLB is an important disease for this crop in Brazil. In our study, we used a phylogeographic approach through the molecular characterization of the translation elongation factor 1-α (TEF1-α) to elucidate the pathogen distribution in Brazil and other countries, besides, search information about diversity sources of this pathogen in coconut palm tree at Brazilian northern, northeast, and southeast...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28437559/chromosomal-evolution-in-large-pelagic-oceanic-apex-predators-the-barracudas-sphyraenidae-percomorpha
#2
R X Soares, M B Cioffi, L A C Bertollo, A T Borges, G W W F Costa, W F Molina
Sphyraena (barracudas) represents the only genus of the Sphyraenidae family and includes 27 species distributed into the tropical and subtropical oceanic regions. These pelagic predators can reach large sizes and, thus, attracting significant interest from commercial and sport fishing. Evolutionary data for this fish group, as well its chromosomal patterns, are very incipient. In the present study, the species Sphyraena guachancho, S. barracuda, and S. picudilla were analyzed under conventional (Giemsa staining, C-banding, and Ag-NOR) and molecular (CMA3 banding, and in situ hybridization with 18S rDNA, 5S rDNA, and telomeric probes) cytogenetic methods...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28437558/genetic-diversity-of-bromeliaceae-species-from-the-atlantic-forest
#3
Y Sheu, A S Cunha-Machado, A B P L Gontijo, F C Favoreto, T B C Soares, F D Miranda
The Bromeliaceae family includes a range of species used for many purposes, including ornamental use and use as food, medicine, feed, and fiber. The state of Espírito Santo, Brazil is a center of diversity for this family in the Atlantic Forest. We evaluated the genetic diversity of five populations of the Bromeliaceae family, including specimens of the genera Aechmea, Billbergia (subfamily Bromelioideae), and Pitcairnia (subfamily Pitcairnioidea), all found in the Atlantic Forest and distributed in the state of Espírito Santo...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28437557/expression-of-catalase-alcohol-dehydrogenase-and-malate-dehydrogenase-in-rot-grains-upon-fungicide-use-on-maize-hybrids-grown-at-different-spacings
#4
E R Kluge, M C Mendes, M V Faria, H O Santos, L A Santos, I E Sandini
In this study, we evaluated the fungicide effect on the incidence of rot grains and expression of catalase (CAT), alcohol dehydrogenase (ADH), and malate dehydrogenase (MDH) enzymes in commercial maize hybrids grown with conventional and reduced spacing in Guarapuava, PR, Brazil. The experiment was designed in random blocks with a 3 × 8-factorial scheme, totaling 24 treatments. The first factor constituted three levels, the first with foliar fungicide application [150.0 g/L trifloxystrobin (15.0%, w/v) + 175...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28437556/inheritance-of-bacterial-spot-resistance-in-capsicum-annuum-var-annuum
#5
L R A Silva, R Rodrigues, S Pimenta, J W S Correa, M S B Araújo, C S Bento, C P Sudré
Since 2008, Brazil is the largest consumer of agrochemicals, which increases production costs and risks of agricultural products, environment, and farmers' contamination. Sweet pepper, which is one of the main consumed vegetables in the country, is on top of the list of the most sprayed crops. The bacterial spot, caused by Xanthomonas spp, is one of the most damaging diseases of pepper crops. Genetic resistant consists of a suitable way of disease control, but development of durable resistant cultivars as well as understanding of plant-bacterium interaction is being a challenge for plant breeders and pathologists worldwide...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28437555/polymorphisms-of-alui-and-hin1i-loci-of-the-igf-1r-gene-and-their-genetic-effects-on-growth-traits-in-bian-chickens
#6
P F Wu, D Wang, C F Jin, X Q Zhang, H Q Wu, L Zhang, F X Ding, K Z Xie, G X Zhang
Growth traits are important economic traits in broiler chicken production. AluI and Hin1I loci are two restriction sites, which are respectively located in exons 2 and 3 of the IGF-1R gene. These two loci are significantly related to the growth traits in Jinghai Yellow chickens. In this study, a correlation analysis was performed between these two loci and the growth traits of Bian chickens. The results showed a G376A mutation at the AluI site and a C919A mutation at the Hin1I site, which respectively resulted in three genotypes AA, AB, and BB in exon 2 and three genotypes CC, CD, and DD in exon 3...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28437554/molecular-identification-of-cetaceans-from-the-west-atlantic-using-the-e3-i5-region-of-coi
#7
L H O Falcão, A S Campos, J E P Freitas, M A A Furtado-Neto, V V Faria
Molecular identification is very useful in cases where morphology-based species identification is not possible. Examples for its application in cetaceans include the identification of carcasses of stranded animals in advanced state of decomposition and body parts that are illegally traded. One DNA region that is often used for molecular identification is the Folmer region of the mitochondrial gene cytochrome c oxidase subunit I (COI) (locus 48 to 705 bp). This locus has been used for the identification of several animal species, including whales and dolphins...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28437553/genotype-x-environment-interaction-on-experimental-hybrids-of-chili-pepper
#8
N S S Cabral, A M Medeiros, L G Neves, C P Sudré, S Pimenta, V J Coelho, M E Serafim, R Rodrigues
In Brazil, cultivation of hybrid plants comprise near 40% of the area grown with vegetables. For Capsicum, hybrids of bell and chili peppers have already exceeded 50% and over 25% of all are commercialized seeds. This study aimed to evaluate new pepper hybrids in two environments, Cáceres, MT, and Campos dos Goytacazes, RJ, Brazil. Nine experimental hybrids of C. baccatum var. pendulum were tested and trials were performed in a randomized block design, with three replications and eight plants per plot. In each environment, plants were assessed for canopy diameter, plant height, number of fruit per plant, mean fruit mass per plant, fruit length and diameter, pulp thickness, and content of soluble solids...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28437552/bcr-abl1-transcript-types-showed-distinct-laboratory-characteristics-in-patients-with-chronic-myeloid-leukemia
#9
A P Vasconcelos, I F Azevedo, F C B C Melo, W B Neves, A C A C Azevedo, R A M Melo
In chronic myeloid leukemia (CML) two main types of messenger RNA (e14a2 and e13a2) can be produced by BCR-ABL1 gene rearrangement. Due to conflicting results, the clinical value of these transcripts remains controversial. The aim of this study was to identify associations of e14a2 and e13a2 transcripts with laboratory variables and also the response to treatment. This study included 203 adult patients with CML treated with Imatinib as first-line drug in a reference hematology center in Northeast Brazil. Clinical and laboratory data were obtained after informed consent...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28437551/association-of-interleukin-6-gene-polymorphisms-and-circulating-levels-with-keloid-scars-in-a-chinese-han-population
#10
X J Zhu, W Z Li, H Li, C Q Fu, J Liu
The aim of the present study is to explore the effect of IL-6 gene polymorphisms on the development of keloid scar (KS) in the Chinese Han population. Genotyping of IL-6 was performed by the polymerase chain reaction (PCR), followed by restriction fragment length polymorphism assays (PCR-RFLP). Serum level of IL-6 was measured using enzyme-linked immunosorbent assay (ELISA). Results indicated that when the IL-6 -572 CC homozygote genotype was used as the reference group, the GG genotype was found to be associated with a significantly increased risk of KS (GG vs CC: OR = 2...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28407196/biotools-an-r-function-to-predict-spatial-gene-diversity-via-an-individual-based-approach
#11
A R da Silva, G Malafaia, I P P Menezes
The gene diversity or expected heterozygosity (HE) is based on the allele frequency and is often used as a measure of genetic variability of populations. Knowing the pattern of spatial distribution of HE can be useful for determining strategies of conservation and sampling of collections of individuals. In addition, it can allow one to detect genetic boundaries in a landscape. We adapted a Wombling method based on assignment tests in a circular moving window extensively sampled over the study area in order to estimate HE at points of a prediction grid...
April 13, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28407195/development-of-trap-primers-for-ricinus-communis-l
#12
K S Simões, S A Silva, E L Machado, H S Brasileiro
The objective of this article was to develop TRAP (target region amplification polymorphism) primers for castor bean, with the goal of making functional markers available for genetic studies about the species. To do this, oligonucleotides were designed based on ESTs, obtained from the NCBI (National Center for Biotechnology Information) databank, which code enzymes involved in metabolic routes of fatty acid synthesis, ricin synthesis, and resistance to castor bean pathogens. The forward primers were designed with the help of the Primer3 software and, for the reverse, six arbitrary primers were used...
April 13, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28407194/genetic-diversity-among-cotton-cultivars-in-two-environments-in-the-state-of-mato-grosso
#13
I G Santos, P E Teodoro, F C Farias, F J C Farias, L P Carvalho, J I S Rodrigues, C D Cruz
Using commercial cultivars to compose crossing blocks in cotton is a promising strategy, because these materials have desirable agronomic and technological characteristics. The objective of this study was to evaluate the genetic diversity among 16 cotton cultivars cultivated in two environments in the State of Mato Grosso, the largest national producer, using agronomical and technological traits. There was significant effect to cultivars for all traits, while genotype x environment interaction was significant only for average boll weight, short fiber index, and maturity of fibers...
April 13, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28407193/a-rapid-and-high-quality-method-for-total-rna-isolation-from-haematococcus-pluvialis
#14
Q L Luo, K P Wang, K Xiao, C G Wang, Z L Hu
Haematococcus pluvialis, as the most potential natural source of astaxanthin, which is a powerful antioxidant with high economic value, has attracted more and more scientific attention in recent years. An in-depth understanding of the mechanism for how H. pluvialis produces astaxanthin requires the intensive investigations on its genetic information. In particular, many reported studies were based on a variety of RNA analyses. However, it is difficult to extract RNA with high quality and quantity from H. pluvialis, because of the blockage from its thick cell wall and contamination by a large quantity of pigments, polysaccharides, and lipids...
April 13, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28407192/population-genetic-characterization-of-the-japanese-oak-silkmoth-antheraea-yamamai-lepidoptera-saturniidae-using-novel-microsatellite-markers-and-mitochondrial-dna-gene-sequences
#15
S R Kim, K Y Kim, J S Jeong, M J Kim, K-H Kim, K H Choi, I Kim
The Japanese oak silkmoth, Antheraea yamamai Guérin-Méneville, 1861 (Lepidoptera: Saturniidae), is an important natural resource of industrial value for silk fiber production. Owing to a lack of geographic and population genetic information, systematic domestication of An. yamamai has not been possible yet. In this study, 10 microsatellite markers developed using next-generation sequencing and two mitochondrial DNA (mtDNA) gene sequences (COI and ND4) were used to investigate the genetic variation and geographic structure of An...
April 13, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28407191/genome-wide-association-analysis-of-body-conformation-traits-in-mexican-holstein-cattle-using-a-mix-of-sampled-and-imputed-snp-genotypes
#16
M E González, V M González, M F Montaño, G E Medina, P Mahadevan, C Villa, R Villa
In arid and semi-arid zones, animal health and production are closely correlated with body conformation traits. These selected traits, in turn, allow livestock to adapt unfavorable soil and environmental conditions. The primary objective of this study was to perform a genome-wide association analysis for a set of sampled and imputed SNPs with 16 conformation traits in a population of Holstein cows from a desert area of Northwestern Mexico. Imputation from 6K to 50K SNPs was performed as a low-cost optimization strategy...
April 13, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28407190/selection-of-morphoagronomic-descriptors-for-the-characterization-of-accessions-of-cassava-of-the-eastern-brazilian-amazon
#17
R S Silva, E F Moura, J T Farias-Neto, C A S Ledo, J E Sampaio
The aim of this study was to select morphoagronomic descriptors to characterize cassava accessions representative of Eastern Brazilian Amazonia. It was characterized 262 accessions using 21 qualitative descriptors. The multiple-correspondence analysis (MCA) technique was applied using the criteria: contribution of the descriptor in the last factorial axis of analysis in successive cycles (SMCA); reverse order of the descriptor's contribution in the last factorial axis of analysis with all descriptors ('O'´p') of Jolliffe's method; mean of the contribution orders of the descriptor in the first three factorial axes in the analysis with all descriptors ('Os') together with ('O'´p'); and order of contribution of weighted mean in the first three factorial axes in the analysis of all descriptors ('Oz')...
April 13, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28407189/molecular-analysis-of-genetic-diversity-among-vine-accessions-using-dna-markers
#18
A F da Costa, P E Teodoro, L L Bhering, F D Tardin, R F Daher, W F Campos, A P Viana, M G Pereira
Viticulture presents a number of economic and social advantages, such as increasing employment levels and fixing the labor force in rural areas. With the aim of initiating a program of genetic improvement in grapevine from the State University of the state of Rio de Janeiro North Darcy Ribeiro, genetic diversity between 40 genotypes (varieties, rootstock, and species of different subgenera) was evaluated using Random amplified polymorphic DNA (RAPD) molecular markers. We built a matrix of binary data, whereby the presence of a band was assigned as "1" and the absence of a band was assigned as "0...
April 13, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28407188/genetic-diversity-of-yacon-accessions-using-issr-markers
#19
R M Lorenzoni, F Menine, E Marques Júnior, F L Oliveira, T C B Soares
Yacon cultivation has been intensified and the investigations of this crop have increased at the scientific, agricultural, and social levels because the roots of yacon show beneficial properties for human health, such as reducing cholesterol and glucose blood levels. Since the investigations involving yacon are very recent, there is little information available in terms of the genetic characterization of the cultivated genotypes. In view of the lack of information on the accessions cultivated in the State of Espírito Santo, Brazil, this pioneering study aimed to characterize 60 accessions cultivated in the state using ISSR yacon markers with emphasis on identifying the genetic diversity among the materials...
April 13, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28407187/genetic-variability-and-expression-of-agro-morphological-traits-among-genotypes-of-coffea-arabica-being-promoted-by-supplementary-irrigation
#20
W N Rodrigues, S V B Brinate, L D Martins, T V Colodetti, M A Tomaz
The use of supplementary irrigation to sustain the plantation during moments of severe water stress or moments of high water demand is becoming almost a norm to keep sustainable crops, especially due to the climate changes and the high frequency in which climate extremes are occurring lately. The objective of this study was to evaluate the agronomic performance and expression of genetic variability among genotypes of Coffea arabica L. cultivated in farming systems with different levels of water supply, focusing on rainfed or supplementary irrigated systems...
April 13, 2017: Genetics and Molecular Research: GMR
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