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Genetics and Molecular Research: GMR

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https://www.readbyqxmd.com/read/28613383/cross-amplification-and-characterization-of-microsatellite-markers-in-alcantarea-patriae-versieux-wand
#1
A G Pereira, U C S Bernardi, V C Manhães, R S Ferreira, F D Miranda
The Alcantarea patriae is a Bromeliaceae endemic to the inselbergs of the Atlantic Forest. This taxon, described in the year of 2007 by Versieux & Wanderley, presents restricted and fragmented distribution outside conservation units. Studies to evaluate the genetic structure of its populations can contribute to the conservation and management strategies for the species. In this study, 31 microsatellite markers, descriptive to six different Bromeliaceae species, were evaluated by cross-amplification tests in 20 individuals of A...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613382/high-genetic-diversity-of-jatropha-curcas-assessed-by-issr
#2
B G Díaz, D M Argollo, M C Franco, S M Nucci, W J Siqueira, D M de Laat, C A Colombo
Jatropha curcas L. is a highly promising oilseed for sustainable production of biofuels and bio-kerosene due to its high oil content and excellent quality. However, it is a perennial and incipiently domesticated species with none stable cultivar created until now despite genetic breeding programs in progress in several countries. Knowledge of the genetic structure and diversity of the species is a necessary step for breeding programs. The molecular marker can be used as a tool for speed up the process. This study was carried out to assess genetic diversity of a germplasm bank represented by J...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613381/expressed-sequence-tags-related-to-nitrogen-metabolism-in-maize-inoculated-with-azospirillum-brasilense
#3
L Pereira-Defilippi, E M Pereira, F M Silva, G V Moro
The relative quantitative real-time expression of two expressed sequence tags (ESTs) codifying for key enzymes in nitrogen metabolism in maize, nitrate reductase (ZmNR), and glutamine synthetase (ZmGln1-3) was performed for genotypes inoculated with Azospirillum brasilense. Two commercial single-cross hybrids (AG7098 and 2B707) and two experimental synthetic varieties (V2 and V4) were raised under controlled greenhouse conditions, in six treatment groups corresponding to different forms of inoculation and different levels of nitrogen application by top-dressing...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613380/influence-of-processing-and-storage-of-integral-grape-juice-vitis-labrusca-l-on-its-physical-and-chemical-characteristics-cytotoxicity-and-mutagenicity-in-vitro
#4
E Düsman, I V Almeida, E P Pinto, L Lucchetta, V E P Vicentini
Integral grape juice is extracted from the grape through processes that allow the retention of their natural composition. However, due to the severity of some processes, fruit juices can undergo changes in their quality. The present study evaluated the cytotoxic and mutagenic effects of integral grape juice by a cytokinesis-blocked micronucleus assay in Rattus norvegicus hepatoma cells (HTC) in vitro. Vitis labrusca L. (variety Concord) were produced organically and by a conventional system, and their juice was extracted by a hot extraction process...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613379/restoration-over-time-and-sustainability-of-schinus-terebinthifolius-raddi
#5
S V Álvares-Carvalho, R Silva-Mann, I B Gois, M F V Melo, A S Oliveira, R A Ferreira, L J Gomes
The success of recovery programs on degraded areas is dependent on the genetic material to be used, which should present heterozygosity and genetic diversity in native and recovered populations. This study was carried out to evaluate the model efficiency to enable the recovery of a degraded area of the Lower São Francisco, Sergipe, Brazil. The target species for this study was Schinus terebinthifolius Raddi. Three populations were analyzed, the recovered area, seed-tree source population, and native tree population border established to the recovered area...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613378/genetic-diversity-of-desmanthus-sp-accessions-using-issr-markers-and-morphological-traits
#6
J C Costa, G G M Fracetto, F J C Fracetto, M V F Santos, M A Lira Júnior
Desmanthus is a genus of forage legumes with high nutritional value, productive potential, and ability to obtain nitrogen in association with diazotrophic bacteria. The use of accurate techniques for genotype identification and characterization is essential for breeding programs. Morphological markers are widely used to know the genetic diversity and the molecular markers are fundamental in these studies. We investigated the genetic diversity among Desmanthus sp genotypes in Pernambuco (Brazilian Northeast State), using morphological traits and ISSR markers...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613377/evaluation-of-genetic-diversity-among-soybean-glycine-max-genotypes-using-univariate-and-multivariate-analysis
#7
M M Oliveira, L B Sousa, M C Reis, E G Silva Junior, D B O Cardoso, O T Hamawaki, A P O Nogueira
The genetic diversity study has paramount importance in breeding programs; hence, it allows selection and choice of the parental genetic divergence, which have the agronomic traits desired by the breeder. This study aimed to characterize the genetic divergence between 24 soybean genotypes through their agronomic traits, using multivariate clustering methods to select the potential genitors for the promising hybrid combinations. Six agronomic traits evaluated were number of days to flowering and maturity, plant height at flowering and maturity, insertion height of the first pod, and yield...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613376/genetic-variability-of-garlic-accessions-as-revealed-by-agro-morphological-traits-evaluated-under-different-environments
#8
E S S Hoogerheide, J A Azevedo Filho, R Vencovsky, M I Zucchi, B W Zago, J B Pinheiro
The cultivated garlic (Allium sativum L.) displays a wide phenotypic diversity, which is derived from natural mutations and phenotypic plasticity, due to dependence on soil type, moisture, latitude, altitude and cultural practices, leading to a large number of cultivars. This study aimed to evaluate the genetic variability shown by 63 garlic accessions belonging to Instituto Agronômico de Campinas and the Escola Superior de Agricultura "Luiz de Queiroz" germplasm collections. We evaluated ten quantitative characters in experimental trials conducted under two localities of the State of São Paulo: Monte Alegre do Sul and Piracicaba, during the agricultural year of 2007, in a randomized blocks design with five replications...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613375/study-on-the-association-of-ugt1a9-gene-c-98t-c-polymorphism-and-mycophenolic-acid-plasma-levels-in-renal-transplant-patients
#9
L R Ruschel, V M Schmitt, A B Silva, C S A Oliveira, K Flach, D O d'Avila, F V Thiesen
Mycophenolate mofetil (MMF) is a prodrug active only after its hydrolysis to mycophenolic acid (MPA). The UGT1A9 enzyme is of special interest since it is the main enzyme involved in the glucuronidation of MPA. Single nucleotide polymorphisms (SNPs) in the UGT1A9 gene may be responsible for individual differences in the pharmacokinetics of MMF. Expression levels and the activity of UGT1A9 may depend on the presence of some SNPs located in the gene promoter region (-2152C>T and -275T>A), as well as changes in the coding region (c...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613374/minimum-number-of-measurements-for-evaluating-soursop-annona-muricata-l-yield
#10
C F B Sánchez, P E Teodoro, S Londoño, L A Silva, L A Peixoto, L L Bhering
Repeatability studies on fruit species are of great importance to identify the minimum number of measurements necessary to accurately select superior genotypes. This study aimed to identify the most efficient method to estimate the repeatability coefficient (r) and predict the minimum number of measurements needed for a more accurate evaluation of soursop (Annona muricata L.) genotypes based on fruit yield. Sixteen measurements of fruit yield from 71 soursop genotypes were carried out between 2000 and 2016...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613373/lack-of-association-between-human-leukocyte-antigen-polymorphisms-rs9277535-and-rs7453920-and-chronic-hepatitis-b-in-a-brazilian-population
#11
V R Z B Pereira, J M Wolf, G Z Stumm, T R Boeira, J Galvan, D Simon, V R Lunge
Hepatitis B virus (HBV) infection is a serious public health problem worldwide. The progression of the disease depends on several host and viral factors and may result in fulminant hepatitis (very rare), acute hepatitis with spontaneous clearance, and chronic hepatitis B infection. Previous studies demonstrated that variations in the human leukocyte antigen (HLA) class II (HLA-DPB1 and HLA-DQB2 genes) are related to the chronic HBV infection. This study aimed to investigate the association of two single nucleotide polymorphism (SNPs), one in the HLA-DPB1 (rs9277535) and one in the HLA-DQB2 (rs7453920), with chronic hepatitis B infection in a southern Brazilian sample...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613372/cleaning-cassava-genotypes-infected-with-cassava-frogskin-disease-via-in-vitro-shoot-tip-culture
#12
M J S Carvalho, E J Oliveira, A S Souza, J S Pereira, M S A S Diamantino, S A S Oliveira
This study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28613371/effect-of-shenqin-biochemical-extract-on-hypoxia-inducible-factor-1%C3%AE-expression-in-ultraviolet-b-irradiated-hacat-cells
#13
X Chen, J Bai, Y Li, X-G Li, G-F Lv, H-Ch Zhang
Hypoxia-inducible factor-1α (HIF-1α) is considered the main transcriptional regulator of the hypoxia-specific cellular and developmental response. This study was performed to investigate the effect of Shenqin biochemical extract (SQBE) on HIF-1α expression in ultraviolet B (UVB)-irradiated HaCaT cells and the possible action mechanisms of SQBE against UVB-induced skin cancer. HaCaT cells in logarithmic growth phase were seeded in Dulbecco's modified Eagle's medium with 10% fetal bovine serum, and conventionally cultured at 37°C with 5% CO2...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28549210/selecting-sugarcane-genotypes-by-the-selection-index-reveals-high-gain-for-technological-quality-traits
#14
L A Silva, P E Teodoro, L A Peixoto, C Assis, K Gasparini, M H P Barbosa, L L Bhering
Sugarcane (Saccharum sp) is one of the most promising crops and researchers have sought for renewable alternative energy sources to reduce CO2 emission. The study of strategies, which allow breeders in the selection of superior genotypes for many traits simultaneously, is important. Therefore, the objectives of this study were: i) to apply path analysis to better understand the relationship between the lignocellulosic traits and technological quality traits with total recoverable sugars (TRS) and ii) to use several multivariate selection indexes to predict the genetic gain and to select superior genotypes in the sugarcane breeding...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28549209/genotypic-correlation-and-path-analysis-in-early-and-super-early-maize-genotypes
#15
B M Alves, A Cargnelutti Filho
The aims of this study were to assess the linear relationships between agronomic and nutritional traits and to identify promising traits for indirect selection in early and super-early maturing maize genotypes. Two trials were run in the 2009/2010 agricultural year, each consisting of a randomized block design with three replications. One trial was run on 36 early maturing maize genotypes and the other on 22 super-early maturing genotypes. Six agronomic traits, 11 protein-nutritional traits, and four energetic-nutritional traits were measured...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28549208/identification-of-reference-genes-for-rt-qpcr-analysis-in-peach-genotypes-with-contrasting-chilling-requirements
#16
N Marini, C B Bevilacqua, M V Büttow, M C B Raseira, S Bonow
Selecting and validating reference genes are the first steps in studying gene expression by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The present study aimed to evaluate the stability of five reference genes for the purpose of normalization when studying gene expression in various cultivars of Prunus persica with different chilling requirements. Flower bud tissues of nine peach genotypes from Embrapa's peach breeding program with different chilling requirements were used, and five candidate reference genes based on the RT-qPCR that were useful for studying the relative quantitative gene expression and stability were evaluated using geNorm, NormFinder, and bestKeeper software packages...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28549207/morphological-descriptors-and-issr-molecular-markers-in-the-evaluation-of-genetic-variability-of-tectona-grandis-genotypes
#17
A M Chimello, J G Jesus, P E Teodoro, A A B Rossi, K L Araújo, T N Marostega, L G Neves, M A A Barelli
This study aimed to evaluate the genetic variability of the teak germplasm bank, using morphological traits and inter-simple sequence repeat molecular markers. Thirty clones were evaluated in a randomized complete block design with three replicates, and each plot was composed of three plants. A joint analysis of quantitative and qualitative variables was performed using the Gower algorithm. Quantitative, qualitative, and molecular variables were analyzed simultaneously using the Ward-MLM procedure. There is genetic variability among the 30 teak genotypes studied, considering the quantitative, qualitative, and molecular variables by the Ward-MLM statistical procedure...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28549206/assessment-of-allelic-diversity-among-drought-resistant-cotton-genotypes-using-microsatellite-markers
#18
A Javaid, F S Awan, F M Azhar, I A Khan
Drought, in conjunction with high temperature, is an important environmental constraint to cotton production. Development of cotton varieties with increased tolerance against adverse environmental conditions has been proposed as effective strategy for ensuring reliable yields. In the present study, 30 simple sequence repeat (SSR) primers were used to estimate genetic divergence among 22 cotton genotypes for drought stress tolerance. Genetic diversity is a prerequisite for developing drought resistant cotton genotypes...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28549205/genetic-divergence-among-corn-hybrids-and-combining-ability-for-agronomic-and-bromatological-traits-of-silage
#19
E Gralak, M V Faria, A S T Figueiredo, D A Rizzardi, M Neumann, M C Mendes, C A Scapim, S Galbeiro
We assessed the impact of genetic divergence and the ability to combine corn hybrids used for the production of silage on the agronomic and bromatological traits of silage quality. We evaluated 18 corn hybrids used as genitors in a circulant diallel scheme in which each genitor hybrid participated in 9 hybrid combinations, and evaluated 100 treatments [18 genitor hybrids, 81 diallelic hybrids, and a commercial check hybrid (DKB330)] in a triple lattice 10 x 10 experimental design in two environments in Brazil...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28549204/setd5-gene-variant-associated-with-mild-intellectual-disability-a-case-report
#20
E Stur, L A Soares, I D Louro
The recent advent of exome sequencing has allowed for the identification of pathogenic gene variants responsible for a variety of diseases that were previously clinically diagnosed, with no underlying molecular etiology. Among these conditions, intellectual disability is a prevalent heterogeneous condition, presenting itself in a large spectrum of intensity, in some cases associated with congenital malformations, behavioral and various other intellectual development alterations. Here we report on a 36-year-old male patient, with a mild intellectual disability that remained undiagnosed at the molecular level for all his life...
May 25, 2017: Genetics and Molecular Research: GMR
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