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Genetics and Molecular Research: GMR

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https://www.readbyqxmd.com/read/28525660/polymorphism-of-mitochondrial-dna-in-the-brazilian-canind%C3%A3-goat-breed
#1
N M V Silva, E C Pimenta Filho, J K G Arandas, M A Gomes Filho, E Ferreira, I Del Cerro, C Fonseca, M N Ribeiro
The success of the geographical distribution of goat populations around the world is a consequence of the adaptive potential of these breeds. Several relevant traits to the success of the species in colonizing different ecosystems (and use by man) evolved before domestication. These features were relevant for the selection of different breeds. Each breed represents a genetic heritage that may be unique and essential for maintaining the species. The objective of this study was to catalog the mtDNA haplotypes of the Brazilian autochthonous Canindé goat breed and to characterize the genetic diversity observed in subpopulations by sequencing a 481-bp fragment corresponding to the first portion of the control region in 178 individuals from 10 herds, sampled in six Brazilian states...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28525659/genetic-variability-in-jatropha-curcas-l-from-diallel-crossing
#2
D O Ribeiro, R Silva-Mann, S V Alvares-Carvalho, E M S Souza, M C Vasconcelos, A F Blank
Physic nut (Jatropha curcas L.) presents high oilseed yield and low production cost. However, technical-scientific knowledge on this crop is still limited. This study aimed to evaluate and estimate the genetic variability of hybrids obtained from dialell crossing. Genetic variability was carried out using ISSR molecular markers. For genetic variability, nine primers were used, and six were selected with 80.7% polymorphism. Genetic similarity was obtained using the NTSYS pc. 2.1 software, and cluster analysis was obtained by the UPGMA method...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28525658/genotyping-of-snps-associated-with-meat-tenderness-comparison-of-two-pcr-based-methods
#3
L E López-Rojas, L Patiño-Cadavid, A López-Herrera, J J Echeverri-Zuluaga
Single nucleotide polymorphisms (SNPs) carried in calpain (CAPN1), calpastatin (CAST), and leptin (LEP) genes are associated with meat tenderness. Due to the economic importance of this meat quality attribute, the development of fast, reliable, and affordable methods to identify bovine carriers of favorable alleles is of great importance for genetic improvement. Currently, PCR-RFLP is accepted as the standard gold method for genotyping SNPs associated with meat tenderness. But these SNPs can be detected by other techniques as high-resolution melting (HRM) analysis - a post-PCR method - that offers several advantages and has great application potential in the meat industry...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28525657/genetic-diversity-of-native-populations-of-croton-tetradenius-baill-using-issr-markers
#4
C S Almeida-Pereira, A V C Silva, R P Alves, R B Feitosa-Alcantara, M F Arrigoni-Blank, S V Alvares-Carvalho, T S Costa, L A S White, V S Pinto, T S Sampaio, A F Blank
Brazil has about 300 Croton species in different types of vegetation. Croton tetradenius Baill., which is endemic to the Northeast region and predominant in the Caatinga vegetation, stands out among the several species of this genus. Considering the importance of knowing the genetic variability of a species, the objective of this study was to analyze the genetic diversity of the genotypes of natural populations of C. tetradenius in the State of Sergipe, using ISSR molecular markers. Forty individuals were sampled in four natural populations of the State of Sergipe, Brazil...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28525656/a-snapshot-on-prokaryotic-diversity-of-the-solim%C3%A3%C2%B5es-river-basin-amazon-brazil
#5
D Toyama, C D Santos-Júnior, L T Kishi, T C S Oliveira, J W Garcia, H Sarmento, F P Miranda, F Henrique-Silva
The Amazon region has the largest hydrographic basin on the planet and 
is well known for its huge biodiversity of plants and animals. However, 
there is a lack of studies on aquatic microbial biodiversity in the 
Solimões River, one of its main water courses. To investigate the 
microbial biodiversity of this region, we performed 16S rRNA gene clone 
libraries from Solimões River and adjacent rivers and lakes. Our question was which microorganisms inhabit the different types of aquatic 
environments in this part of the basin, and how diversity varies among 
these environments (rivers and lakes)...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28525655/genetic-parameters-and-path-analysis-in-cowpea-genotypes-grown-in-the-cerrado-pantanal-ecotone
#6
K V Lopes, P E Teodoro, F A Silva, M T Silva, R L Fernandes, T C Rodrigues, T C Faria, A M Corrêa
Estimating genetic parameters in plant breeding allows us to know the population potential for selecting and designing strategies that can maximize the achievement of superior genotypes. The objective of this study was to evaluate the genetic potential of a population of 20 cowpea genotypes by estimating genetic parameters and path analysis among the traits to guide the selection strategies. The trial was conducted in randomized block design with four replications. Its morphophysiological components, components of green grain production and dry grain yield were estimated from genetic use and correlations between the traits...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28525654/molecular-detection-by-analysis-of-the-16s-rrna-gene-of-fecal-coliform-bacteria-from-the-two-korean-apodemus-species-apodemus-agrarius-and-a-peninsulae
#7
M M Rahman, K B Yoon, S J Lim, M G Jeon, H J Kim, H Y Kim, J Y Cho, H M Chae, Y C Park
Wild mouse feces can disseminate zoonotic microorganisms throughout a farm, which is a great threat to human health and can lead to economic loss through contaminated agricultural produce. To assess the microbial communities, especially fecal coliform bacteria, we used two methods. First, we isolated bacterial colonies onto the common media LB (lactose broth) agar, TSA (tryptic soy agar), and MRS (de Man, Rogosa, and Sharpe) agar, and then randomly select colonies from each plate and stocked them to the mother plate for genomic DNA isolation...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28525653/predicted-philogeny-secondary-conformational-structure-and-epitope-antigenicity-of-immunological-sequences-in-poultry
#8
L J Lara, A P Peconick, É J Fassani, A M P Júnior, P R B Chalfun, D L Raymundo, T A Barçante, J M de P Barçante
Poultry production is faced with different types of stresses that are responsible for issues of animal welfare as well as for economic losses. Moreover, the immunity decreases when animals are stressed. In silico analyses are important in reducing the cost and in increasing the accuracy of scientific results. A bioinformatics tool was used to perform ontology studies on 15 different immunological sequences of poultry. The mRNA structures and sequences with maximum antigenic residues were also predicted. No homology was found between the sequences of poultry and mammals...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28525652/determination-of-scn1a-genetic-variants-in-mexican-patients-with-refractory-epilepsy-and-dravet-syndrome
#9
R E Jiménez-Arredondo, A J L Brambila-Tapia, F M Mercado-Silva, M T Magaña-Torres, L E Figuera
Mutations in the SCN1A gene can result in syndromes associated with epilepsy, including the Dravet syndrome (DS). However, the prevalence of such mutations in these diseases varies widely between different studies, and has not been examined in Mexican patients with epilepsy. Therefore, the objective of this study was to determine the frequency of SCN1A mutations (in the exon 26) in a cohort of Mexican patients with DS and refractory epilepsy (RE). We recruited 24 Mexican patients (14 males and 10 females), of which 15 were diagnosed with RE and 9 were diagnosed with DS...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28525651/selecting-elephant-grass-families-and-progenies-to-produce-bioenergy-through-mixed-models-reml-blup
#10
E V Rodrigues, R F Daher, A Dos Santos, M Vivas, J C Machado, G do A Gravina, Y P de Souza, A K Vidal, A Dos S Rocha, R S Freitas
Brazil has great potential to produce bioenergy since it is located in a tropical region that receives high incidence of solar energy and presents favorable climatic conditions for such purpose. However, the use of bioenergy in the country is below its productivity potential. The aim of the current study was to select full-sib progenies and families of elephant grass (Pennisetum purpureum S.) to optimize phenotypes relevant to bioenergy production through mixed models (REML/BLUP). The circulating diallel-based crossing of ten elephant grass genotypes was performed...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28525650/detection-of-damage-on-single-or-double-stranded-dna-in-a-population-exposed-to-arsenic-in-drinking-water
#11
J Jiménez-Villarreal, D I Rivas-Armendariz, C P Pineda-Belmontes, N D Betancourt-Martínez, M A Macías-Corral, A J Guerra-Alanis, M S Niño-Castañeda, J Morán-Martínez
Different studies have suggested an association between arsenic (As) exposure and damage to single-stranded DNA by reactive oxygen species derived from the biotransformation of arsenic. The single strand damages are converted to double strand damage upon interaction with ultraviolet radiation. Analysis of genomic integrity is important for assessing the genotoxicity caused by environmental pollutants. In this study, we compared the concentration of As in drinking water, nutritional status, lifestyle variables, and the level of genotoxicity in an exposed population and a control group...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28525649/genome-sequence-compression-based-on-optimized-context-weighting
#12
M Chen, J J Shao, X M Jia
Context weighting is an important technology for genome compression. In this study, we discuss the relationship between the weighting of context models and the weighting of the description lengths corresponding to their respective context models. It indicates that weighting of context models is equivalent to the weighting of their description lengths. With these discussions, we present the weights optimization algorithm based on the minimum description length, and suggest implementing the least-square algorithm for the optimization of the weights...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28510258/diversity-of-plant-growth-promoting-bacteria-associated-with-sugarcane
#13
J E L Antunes, M C C P Lyra, F J Ollero, A D S Freitas, L M S Oliveira, A S F Araújo, M V B Figueiredo
The sugarcane (Saccharum spp) presents economic importance, mainly for tropical regions, being an important Brazilian commodity. However, this crop is strongly dependent on fertilizers, mainly nitrogen (N). This study assessed the plant growth-promoting bacteria (PGPB) associated with sugarcane that could be used as a potential inoculant to the crop. We evaluated the genetic diversity of PGPB in the plant tissue of sugarcane varieties (RB 867515, RB 1011, and RB 92579). The primer BOX-A1R was used to differentiate the similar isolated and further sequencing 16S rRNA ribosomal gene...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28510257/strategy-for-selection-of-soybean-genotypes-tolerant-to-drought-during-germination
#14
S A G Dantas, F C S Silva, L J Silva, F L Silva
Water deficit is the main reason for instability in the context of soybean culture. The development of strategies for the selection of more tolerant genotypes is necessary. These strategies include the use of polyethylene glycol 6000 solutions (PEG-6000) for conducting the germination test under conditions of water restriction. Thus, the objective of this study was to determine the osmotic potential and the main characteristics that promote the discrimination of soybean genotypes with regard to water stress tolerance during germination and the vigor test...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28510256/interregional-cytogenetic-comparisons-in-halichoeres-and-thalassoma-wrasses-labridae-of-coastal-and-insular-regions-of-the-southwestern-atlantic
#15
K D J Amorim, M B Cioffi, L A C Bertollo, R X Soares, L L Calado, A T Borges, G W W F Costa, W F Molina
The distribution patterns of marine biodiversity are complex, resulting from vicariant events and species dispersion, as well as local ecological and adaptive conditions. Furthermore, the wide geographic distribution of some species may be hindered by biogeographical barriers that can interfere in the gene flow. Cytogenetic analyses in marine fishes, especially those involving populations in small remote insular environments, remain scarce. In the Western Atlantic, species of wrasses from the genera Halichoeres and Thalassoma occur in biogeographic arrangements that make it possible to analyze cytogenetic patterns between coastal and widely separated island populations...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28510255/adaptability-and-stability-of-soybean-cultivars-for-grain-yield-and-seed-quality
#16
K B Silva, A T Bruzi, E V Zambiazzi, I O Soares, J L A R Pereira, M L M Carvalho
This study aimed at verifying the adaptability and stability of soybean cultivars, considering the grain yield and quality of seeds, adopting univariate and multivariate approaches. The experiments were conducted in two crops, three environments, in 2013/2014 and 2014/2015 crop seasons, in the county of Inconfidentes, Lavras, and Patos de Minas, in the Minas Gerais State, Brazil. We evaluated 17 commercial soybean cultivars. For adaptability and stability evaluations, the Graphic and GGE biplot methods were employed...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28510254/effects-of-mutant-tdp-43-on-the-nrf2-are-pathway-and-protein-expression-of-mafk-and-jdp2-in-nsc-34-cells
#17
Y P Tian, F Y Che, Q P Su, Y C Lu, C P You, L M Huang, S G Wang, L Wang, J X Yu
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease that affects motor neurons and lacks an effective treatment. The disease pathogenesis has not been clarified at present. Pathological transactive response DNA-binding protein 43 (TDP-43) plays an important role in the pathogenesis of ALS. Nuclear translocation of nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) is found in a mutant TDP-43 transgenic cell model, but its downstream antioxidant enzyme expression is decreased. To elucidate the specific mechanism of Nrf2/ARE (antioxidant responsive element) signaling dysfunction, we constructed an ALS cell model with human mutant TDP-43 using the NSC-34 cell line to evaluate the impact of the TDP-43 mutation on the Nrf2/ARE pathway...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28510253/feline-immunodeficiency-virus-and-feline-leukemia-virus-frequency-and-associated-factors-in-cats-in-northeastern-brazil
#18
L C Lacerda, A N Silva, J S Freitas, R D S Cruz, R A Said, A D Munhoz
Our aims were to determine the frequencies of feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) in owned and stray cats in the northeastern region of Brazil, ascertain the status of FeLV infection, and investigate potential associated factors among the owned cats. Blood samples from 200 asymptomatic owned cats and 30 stray cats were processed using nested PCR and commercial immunochromatographic tests to diagnose infections. To evaluate the factors associated with FIV and/or FeLV in owned cats, a semi-structured interview was conducted with each owner about the animal's environment, and these data were subjected to unconditional logistic regression...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28510252/assessing-non-additive-effects-in-gblup-model
#19
I C Vieira, J P R Dos Santos, L P M Pires, B M Lima, F M A Gonçalves, M Balestre
Understanding non-additive effects in the expression of quantitative traits is very important in genotype selection, especially in species where the commercial products are clones or hybrids. The use of molecular markers has allowed the study of non-additive genetic effects on a genomic level, in addition to a better understanding of its importance in quantitative traits. Thus, the purpose of this study was to evaluate the behavior of the GBLUP model in different genetic models and relationship matrices and their influence on the estimates of genetic parameters...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28510251/short-term-effects-of-green-tea-chronotherapy-on-the-metabolic-homeostasis-of-mice-on-different-diets
#20
R P Amorim, I P Daher, F P D Arcangelo, M F Bellini, W A Orcini, G R Sarria, R L Peruquetti
Biological rhythms can be defined as changes in physiological or behavioral variables that repeat at certain time intervals. Rhythms that last approximately 24 h are referred to as circadian rhythms. Modern lifestyles have drastically affected human habits, as well as the population's eating habits. These changes have generated an epidemic of metabolic syndromes, such as obesity and diabetes. In an attempt to combat obesity, populations have attempted to use many different herbal remedies and plant-based drugs, the most common of which is Camellia sinensis, or green tea...
May 10, 2017: Genetics and Molecular Research: GMR
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