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Genetics and Molecular Research: GMR

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https://www.readbyqxmd.com/read/28198515/development-and-characterization-of-microsatellite-markers-for-solen-grandis-using-illumina-sequencing-approach
#1
Y W Feng, H L Jiang, Y T Liu, X P Wu, X Q Liu, X M Wei
Solen grandis is an important economic and overexploited bivalve species. In order to perform its fine-scale genetic analyses, 105 pairs of microsatellites with polymorphism were identified through Illumina Hiseq platform and bioinformatic assembly technology in this study. The estimated fragment size ranged from 100 to 268 bp and the number of alleles per locus varied between 2 and 23. Observed and expected heterozygosities varied from 0.0667 to 1.0000 and 0.0966 to 0.9492, respectively. Fourteen loci deviated significantly from Hardy-Weinberg equilibrium after Bonferroni correction...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198514/genetic-diversity-among-exotic-cotton-accessions-as-for-qualitative-and-quantitative-traits
#2
L P de Carvalho, F J C Farias, J I S Rodrigues, N D Suassuna, P E Teodoro
Studying genetic diversity among a group of genotypes is important in genetic breeding because identifying hybrid combinations of greater heterotic effect also increases the chance of obtaining plants with favorable allele combinations in an intra-population selection program. The objective of this study was to compare different types of long and extra-long staple cotton and their genetic diversity in relation to the fiber traits and some agronomic traits in order to grant breeding programs. Diversity analysis among 29 cotton accessions based on qualitative and quantitative traits and joint including qualitative and quantitative traits was performed...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198513/sybr-safe-tm-efficiently-replaces-ethidium-bromide-in-aspergillus-fumigatus-gene-disruption
#3
H M S Canela, L A Takami, M E S Ferreira
Invasive aspergillosis is a disease responsible for high mortality rates, caused mainly by Aspergillus fumigatus. The available drugs are limited and this disease continues to occur at an unacceptable frequency. Gene disruption is essential in the search for new drug targets. An efficient protocol for A. fumigatus gene disruption was described but it requires ethidium bromide, a genotoxic agent, for DNA staining. Therefore, the present study tested SYBR safe(TM), a non-genotoxic DNA stain, in A. fumigatus gene disruption protocol...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198512/case-of-escape-in-cassava-manihot-esculenta-crantz
#4
N M A Nassar, M Mendonza
Two cassava escapes where collected from cultivated fields near natural habitat in Bolivia. They are described morphologically and analyzed cytogenetically in this study. It is suggested that they are the product of backcrosses of cassava interspecific hybrids with the cultigen itself, and that selective conditions have developed in which certain forms of cassava segregates have adapted to grow wildly in natural habitats near cassava fields. These segregates may hybridize with cultivated cassava upon coming in contact with such varieties...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198511/rapid-communication-genetic-diversity-and-differentiation-in-natural-populations-of-arapaima-gigas-from-lower-amazon-revealed-by-microsatellites
#5
P F Fazzi-Gomes, N Melo, G Palheta, S Guerreiro, M Amador, A K Ribeiro-Dos-Santos, S Santos, I Hamoy
Genetic variability is one of the important criteria for species conservation decisions. This study aimed to analyze the genetic diversity and the population differentiation of two natural populations of Arapaima gigas, a species with a long history of being commercially exploited. We collected 87 samples of A. gigas from Grande Curuai Lake and Paru Lake, located in the Lower Amazon region of Amazônia, Brazil, and genotyped these samples using a multiplex panel of microsatellite markers. Our results showed that the populations of A...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198510/letter-partial-diallel-crosses-for-predicting-yield-of-semiexotic-maize-populations
#6
C M Silva, J B Miranda Filho, U C Mendes, E F Reis
In Brazil, the total production of maize has increased continuously due to the advances in technology but also to the new frontiers for the maize crop, including the expansion of the second crop (safrinha; usually maize after soybean). Therefore, the exploitation of new sources of germplasm seems to be imperative to attend the actual and future demands for modern cultivars adapted to the greatly variable environments available to the maize crop. The present study was based on a partial diallel (intergroup) cross to provide quantitative information on the potential of two groups of varieties (NAP - exotics and HG - local) and their combining abilities to direct the synthesis of new populations to be used in hybrid crosses or as a base for breeding programs...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198509/multivariate-approach-in-popcorn-genotypes-using-the-ward-mlm-strategy-morpho-agronomic-analysis-and-incidence-of-fusarium-spp
#7
R N F Kurosawa, A T do Amaral Junior, F H L Silva, A Dos Santos, M Vivas, S H Kamphorst, G F Pena
The multivariate analyses are useful tools to estimate the genetic variability between accessions. In the breeding programs, the Ward-Modified Location Model (MLM) multivariate method has been a powerful strategy to quantify variability using quantitative and qualitative variables simultaneously. The present study was proposed in view of the dearth of information about popcorn breeding programs under a multivariate approach using the Ward-MLM methodology. The objective of this study was thus to estimate the genetic diversity among 37 genotypes of popcorn aiming to identify divergent groups associated with morpho-agronomic traits and traits related to resistance to Fusarium spp...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198508/genetic-tests-for-low-and-middle-income-countries-a-literature-review
#8
P E Maltese, E Poplavskaia, I Malyutkina, F Sirocco, A Bonizzato, N Capodicasa, S Y Nicoulina, A Salmina, N Aksutina, M Dundar, T Beccari, S Cecchin, M Bertelli
The aim of this review is to describe a series of ten genetic diseases with Mendelian inheritance pattern in people of low- or middle-income countries, which can be easily identified with simple and affordable methods. Recent information shows that although genetic diseases account for more than 10% of infant mortality in such countries, testing, counseling, and treatment of genetic diseases is not a priority. The selection criteria for the genetic tests that are discussed in this review are: i) the frequency of the genetic disease in the general population, ii) the cost and ease of execution, and iii) the report of validated methods in the literature for the diagnosis of these diseases...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198507/assessment-of-genetic-diversity-of-cotton-genotypes-for-various-economic-traits-against-cotton-leaf-curl-disease-clcud
#9
M Javed, S B Hussain, M Baber
In Pakistan, cotton crop has been under enormous threat of cotton leaf curl disease (CLCuD) over the last four decades. In order to estimate genetic diversity in cotton germplasm CLCuD resistance, we assessed 100 cotton genotypes for their CLCuD resistance/tolerance and other related agronomical traits. Various statistical analytical tools, including correlation analysis, cluster analysis, and principal component analysis (PCA), were used to select the best genotypes. These genotypes can be used in future breeding programs to generate CLCuD resistant varieties...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198506/inhibition-of-mitochondrial-calcium-uptake-1-in-drosophila-neurons
#10
P G M'Angale, B E Staveley
The mitochondrial calcium uptake 1 (MICU1) is a regulatory subunit of the mitochondrial calcium uniporter that plays an important role in calcium sensing. It contains two EF-hand domains that are well conserved across diverse species from protozoa to plants and metazoans. The loss of MICU1 function in mammals is attributed to several neurological disorders that involve movement dysfunction. The CG4495 gene in Drosophila melanogaster was identified as a putative homolog of MICU1 in the HomoloGene database of the National Centre for Biotechnology Information (NCBI)...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198505/migration-and-invasion-enhancer-1-mien1-is-overexpressed-in-breast-cancer-and-is-a-potential-new-therapeutic-molecular-target
#11
H-B Zhao, X-F Zhang, H-B Wang, M-Z Zhang
Migration and invasion enhancer 1 (MIEN1) is a membrane-anchored protein that is highly expressed in various types of cancer, and is correlated with the PI3K/AKT pathway. The aim of this study was to investigate the expression of MIEN1 and its clinical pathological significance in breast cancer. We used immunohistochemical staining to examine the expression of MIEN1 in 40 samples of human breast cancer tissue and 10 samples taken from regions adjacent to normal breast tissue. The rate of detection of MIEN1 protein was 67...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198504/complete-mitochondrial-genome-of-the-amur-hedgehog-erinaceus-amurensis-erinaceidae-and-higher-phylogeny-of-the-family-erinaceidae
#12
N H Kim, S J Lim, H M Chae, Y C Park
We sequenced and characterized the complete mitogenome (KX964606) of the Amur hedgehog Erinaceus amurensis to provide more data for comparative mitogenomics of the genus Erinaceus (Erinaceidae). The mitogenome of E. amurensis is a circular molecule 16,941 bp long, consisting of a control region and a conserved set of 37 genes containing 13 protein-coding genes, 22 tRNA genes, and two rRNA genes (12S rRNA and 16S rRNA). The mitogenome of E. amurensis is AT-biased, with a nucleotide composition of 33.9% A, 21...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198503/relationship-between-lymphocyte-dna-fragmentation-and-dose-of-iron-oxide-fe2o3-and-silicon-oxide-sio2-nanoparticles
#13
J Jiménez-Villarreal, D I Rivas-Armendáriz, R D Arellano Pérez-Vertti, E Olivas Calderón, R García-Garza, N D Betancourt-Martínez, L B Serrano-Gallardo, J Morán-Martínez
At present, the use of nanoparticles is a controversial topic, especially when analyzing their effects in human tissues. Nanoparticles (NPs) can cause oxidative stress by increasing membrane lipids peroxidation and reactive oxygen species, and decreasing intracellular glutathione. Oxidative stress plays an important role in cell signaling and inflammatory responses. It can result in genotoxicity, affect cell proliferation, and induce DNA damage. The objective of this study is to evaluate the genotoxic potential of NPs in lymphocyte DNA...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198502/extraction-of-proteins-and-preliminary-characterization-of-physicochemical-properties-in-toona-sinensis-fruit
#14
W-Z Li, W-N Han, B Liu, S-H Ding, X-K Zhang, R-S Wang
We investigated the extraction of Toona sinensis fruit proteins and preliminarily characterized their physicochemical properties. The results showed that optimal extraction occurred under conditions of pH 10.5, a duration of 40 min, a liquid-to-solid ratio of 25:1, and a temperature of 40°C by an orthogonal design using T. sinensis fruit protein as the index and single factor. The total nitrogen content was 13.8 g/100 g and included 17 different amino acids. The glutamate level was highest at 35.37%, followed by arginine at 15...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198501/effect-of-gjb2-235delc-and-30-35delg-genetic-polymorphisms-on-risk-of-congenital-deafness-in-a-chinese-population
#15
Y Xiong, M Zhong, J Chen, Y L Yan, X F Lin, X Li
Congenital deafness is a serious and irreversible condition in humans. The GJB2 gene is implicated in the pathogenesis of autosomal recessive nonsyndromic hearing loss. Its 235delC and 30-35delG polymorphisms are reported to be associated with risk of hereditary deafness. However, the effect of the interaction between GJB2 235delC and 30-35delG and environmental factors on congenital deafness has not been described. Therefore, we performed a case-control study to investigate the influence of these polymorphisms on congenital deafness risk, and their interaction with maternal and other environmental factors in the development of this disease...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198500/molecular-characterization-expression-and-functional-analysis-of-chicken-traf6
#16
J Jin, J S Ran, C W Yang, X S Jiang, Y G Zhou, Z Q Feng, Y Wang, D Lan, P Ren, Y P Liu
Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial adaptor molecule of the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily, which can trigger downstream signaling cascades involved in innate immunity. The function of TRAF6 has been clarified in mammals but is poorly understood in chicken. In our study, we investigated TRAF6 function in birds, particularly in chicken innate immune responses, by cloning and characterizing chicken TRAF6 (chTRAF6). The full-length coding sequence of chTRAF6 comprised 1638 bp and encoded a 545-amino acid protein, which shares high sequence similarity with TRAF6 of other species and consists of four structurally conserved domains...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198499/cloning-and-expression-of-class-i-chitinases-in-hami-melon-after-penicillium-infection
#17
F Y Jiang, B B Shui, F X Tang, C H Shan
Chitinases are important disease-related proteins that play critical roles in plant defense against disease. To investigate the function of chitinases in the resistance of Hami melon to Penicillium infection, the gene encoding chitinases, HmCHT-2, was cloned and RT-PCR was used to measure expression levels of HmCHT-2. When the Hami melon was infected by Penicillium sp after 0, 12, 36, 48, 60, and 72 h. The results showed that comparing to the control group, the time of expression levels reaching to the peak delayed and the expression levels maintained at a significantly high level for a longer time...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198498/early-selection-of-sugarcane-using-path-analysis
#18
R P Barbosa, F Alcântara Neto, L M Gravina, G A Gravina, M G T Portela, A A C Bezerra
The aim of this study was to analyze sugarcane (Saccharum officinarum) biometric and technological data, obtained at different timepoints, using path analysis. The experiment was conducted in União, PI, Brazil, and evaluated 12 sugarcane genotypes (RB036066, RB9438, RB935744, RB021764, RB021754, RB021534, RB966229, RB977540, RB863129, and RB987935, and the varieties RB92579 and RB867515 as controls) in a randomized block design with four replications. Data were collected at six timepoints that were spaced 30 days apart (90, 120, 150, 180, 210, and 240 days)...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198497/nested-polymerase-chain-reaction-technique-for-the-detection-of-gpc3-and-afp-mrna-in-liver-cancer-micrometastases
#19
J Luo, K Yang, Y G Wen
The incidence of liver cancer has gradually risen to a high level in China, and tumor metastasis occurs via multiple pathways. Alpha fetal protein (AFP) is the main biomarker of liver cancer micrometastases. A recent study showed that glypican-3 (GPC3), which is abundant in hepatoma cells, has promising specificity and could be used to determine the presence of malignant cells. The nested polymerase chain reaction (PCR) technique is superior in experimental sensitivity. Using rat models of liver cancer in the current study, we utilized nested PCR to detect Gpc3 and Afp mRNA to determine their relationship with liver cancer micrometastases...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28198496/association-between-gstm1-gstt1-and-gstp1-polymorphisms-and-gastric-cancer-risk-and-their-interactions-with-environmental-factors
#20
Z H Chen, J F Xian, L P Luo
Glutathione S-transferase (GST) is an important member of phase II metabolic enzymes; GSTM1, GSTT1, and GSTP1 belong to three subfamilies of the GST enzyme. Polymorphisms in GSTM1, GSTT1, and GSTP1 could affect detoxification processes, and increase individuals' susceptibility to cancers. We aimed to investigate the association between GSTM1, GSTT1, and GSTP1 polymorphisms and the risk of gastric cancer in a Chinese population. In addition, we also examined the effect of gene-environmental interactions, and their effect on risk of this cancer...
February 8, 2017: Genetics and Molecular Research: GMR
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