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Gene Expression Patterns: GEP

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https://www.readbyqxmd.com/read/28634012/different-expression-of-sox17-gene-during-gametogenesis-between-scallop-chlamys-farreri-and-vertebrates
#1
Shaoshuai Liang, Zhifeng Zhang, Dandan Yang, Yingying Chen, Zhenkui Qin
SOX17, a member of SRY-related high-mobility-group box (SOX) family, involves in endoderm formation, angiogenesis and carcinogenesis, and its expression characteristics are different in spermatogenesis among several vertebrates. In this study, we cloned a full-length cDNA sequence of sox17 from Zhikong scallop Chlamys farreri, and determined its expression characteristics in gonad at mRNA and protein levels. The cDNA sequence was 2802 bp in length, predicted to encode a protein of 511 amino acids and contained a conservative HMG-box of SOX family, while lacked the C-terminal region of SOX17 comparing to vertebrates...
June 17, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28629960/molecular-cloning-and-mrna-expression-pattern-of-sox4-in-paramisgurnus-dabryanus
#2
Xiaohua Xia, Ruyan Wan, Weiran Huo, Linxia Zhang, Xiaopei Xia, Zhongjie Chang
Sox4 belonged to the SoxC subfamily of the Sox family, which play important roles in the development of the vertebrate gonad and nervous system. A Sox4 homologue was cloned from brain of Paramisgurnus dabryanus by using homologous cloning and rapid amplification of cDNA ends (RACE), designated as PdSox4. The full-length cDNA was 2163bp, containing the 759bp 5'-untranslated region, 267bp 3'-untranslated region and encoding a putative protein of 378 amino acids with a characteristic high mobility group box (HMG-box) DNA-binding domain of 79 amino acids with the specific motif (RPMNAFMVW)...
June 16, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28625897/differential-expression-of-long-noncoding-rnas-in-congenital-microtia
#3
Ling Zhang, Lin Lin, Yu-Peng Song, Bo Pan, Qing-Hua Yang, Hai-Yue Jiang
OBJECTIVE: To analyse lncRNA expression profiles in microtia using bioinformatics analysis. METHODS: We examined lncRNA expression profiles in residual ear cartilage and normal ear cartilage from individual congenital microtia patients. RESULTS: The gene chips used in this study included 30586 lncRNAs and 26109 mRNA probes. Intotal, 180 lncRNAs with differential expression weredetected in the residual ear cartilage compared with the normal cartilage, including 74 up-regulated and 106down-regulated lncRNAs...
June 15, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28625896/lateralization-of-housekeeping-genes-in-the-brain-of-one-day-old-chicks
#4
Jacek Lenart, Klaudia Kogut, Elzbieta Salinska
Real-time quantitative PCR is an exceptionally sensitive method that can detect even very small differences in gene expression and, as such, it is essential to use suitable reference genes. Domestic chickens are used in a wide range of studies including neurobiology, behavior, ecology and disease transmission. In recent avian gene expression experiments, 18S (18S ribosomal RNA), beta actin (ACTB) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) have frequently been used; however, there is not enough evidence that these reference genes are suitable for all types of experiments...
June 15, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28625895/expression-analyses-of-salinity-stress-associated-ests-in-aeluropus-littoralis
#5
Tayebeh Kakeshpour, Sajad Rashidi Monfared, Amin Ebrahimi, Arman Beyraghdar Kashkooli, Esmaeil Ebrahimie
Salinity is among the most important abiotic stresses affecting crop production throughout the earth. Halophyte plants can sustain high salinity levels, therefore elucidating molecular mechanisms underlying their salinity resistance is beneficial for crop improvement. Aeluropus littoralis, a halophyte weed, is a great genetic resource for this purpose. Isolated expressed sequence taq (EST) sequences from A. littoralis under salinity stress, have given us the chance to find and analyze transcripts of genes involved in response to salinity...
June 15, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28624368/conservation-of-bmp2-4-expression-patterns-within-the-clade-branchiostoma-amphioxus-resolving-interspecific-discrepancies
#6
Luok Wen Yong, Stéphanie Bertrand, Jr-Kai Yu, Hector Escriva, Nicholas D Holland
In 2016, Kaji et al. concluded that the amphioxus mouth has the quality of a coelomoduct and is, therefore, not homologous to the oral opening of any other animal. They studied a Japanese population of Branchiostoma japonicum and based their conclusion, in part, on the larval expression of BMP2/4 in cells that reportedly joined the rim of the forming mouth. They did not detect transcription of that gene in any other tissues in the anterior region of the larva. Their results were almost the inverse of findings for B...
June 14, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28610924/embryonic-expression-patterns-of-eukaryotic-endou-ribonuclease-family-gene-endouc-in-zebrafish
#7
Hung-Chieh Lee, Chuan-Yang Fu, Chih-Wei Zeng, Huai-Jen Tsai
Endou proteins belong to the Eukaryotic EndoU ribonuclease family of enzymes that present high sequence homology with the founding member XendoU domain. The enzymatic activity and three-dimensional structure of some Endou proteins have been previously reported. However, their molecular structure and gene expression patterns during embryogenesis remain to be elucidated. Therefore, we took zebrafish (Danio rerio) endouC as the model to study molecular structure and gene expression dynamics at different developmental stages...
June 10, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28603023/deciphering-the-role-of-microrna-a-step-by-step-guide
#8
Siddharth Manvati, Kailash Chandra Mangalhara, Juveria Khan, Geeta Lal Pathania, Srishti Kaul, Monika Kaushik, Ankita Arora, Pawan K Dhar
MicroRNAs (miRNAs), are small non-coding RNAs of approximately 22 nucleotides in length, playing an important role in regulating gene expression post-transcriptionally. Understanding the effect of miRNA regulation in a pathway-specific manner unravels the approaches adopted to apprehend biological mechanisms, the information, which is scanty for researchers, not primed already for miR related research. Here, we describe a quick perspective in 5 steps with probable approaches and assays at every level to unravel the specific role of a microRNA, miR-145a-5p, as an example...
June 8, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28583421/gene-expression-profiles-in-mouse-cumulus-cells-derived-from-in%C3%A2-vitro-matured-oocytes-with-and-without-blastocyst-formation
#9
Yixin Xu, Tuanping Zhou, Li Shao, Bei Zhang, Kailu Liu, Chao Gao, Li Gao, Jiayin Liu, Yugui Cui, Ri-Cheng Chian
Cumulus cells (CCs) are considered as an important source to predict oocyte quality. Despite numerous candidate genes in CCs have been identified for embryonic developmental competence, the results are inconsistent. The next generation RNA-sequencing was used to investigate the transcriptomic differences in CCs from in vitro matured oocytes did or did not develop to blastocyst stage following in vitro fertilization (IVF). In our study, the corresponding mouse oocytes were traced using a single-cell tracking system, and CCs were pooled into groups based on the embryonic developmental outcomes...
June 2, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28479482/embryonic-expression-of-festina-lente-fel-a-novel-maternal-gene-in-the-oligochaete-annelid-tubifex-tubifex
#10
Takuma Nakamura, Inori Shiomi, Takashi Shimizu
We have cloned and characterized the expression of a novel maternal gene festina lente (designated Ttu-fel) from the clitellate annelid Tubifex tubifex. Northern blot analyses have shown that Ttu-fel mRNA is approximately 8 kbp in length and that its expression is restricted to oocytes undergoing maturation division and early embryos up to 22-cell stage. Maternal transcripts of Ttu-fel are first detected in oocytes in the ovary of young adults (ca. 40 days after hatching); its expression continues in growing oocytes in the ovisac...
May 4, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28465207/characterization-of-sgo1-expression-in-developing-and-adult-mouse
#11
Andrew T Song, Antonella Galli, Severine Leclerc, Stanley Nattel, Craig Mandato, Gregor Andelfinger
SGO1 has been characterized in its function in correct cell division and its role in centrosome cohesion in the nucleus. However, its organ-specific maturation-related expression pattern in vivo remains largely uncharacterized. Here, we show clear SGO1 expression in post-developmental neuronal cells and cytoplasmic localisation in nucleated cells with a transgenic mice model and immunohistochemistry of wild type mice. We demonstrate extranuclear expression of Sgo1 in the developing heart and gut, which have been shown to be dysregulated in humans with homozygous SGO1 mutation...
April 29, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28442438/dorsal-fin-development-in-flounder-paralichthys-olivaceus-bud-formation-and-its-cellular-origin
#12
Jie Chen, Xiaoyu Liu, Xiaohua Yao, Fei Gao, Baolong Bao
The development of the median fin has not been investigated extensively in teleosts, although in other fishes it has been proposed that it involves the same genetic programs operating in the paired appendages. Adult median fins develop from the larval bud; therefore an investigation of fin bud formation and its cellular origin is essential to understanding the maturation mechanisms. In Paralichthys olivaceus, skeletogenesis proceeds from an anterior to posterior direction providing a good opportunity to study the formation of dorsal fin bud...
April 22, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28414113/sept8a-and-sept8b-mrna-expression-in-the-developing-and-adult-zebrafish
#13
Constantin Berger, Frederik Helmprobst, Prisca Chapouton, Christina Lillesaar, Christian Stigloher
Septins are highly conserved GTP-binding proteins involved in numerous cellular processes. Despite a growing awareness of their roles in the cell biology, development and signal transmission in nervous systems, comparably little is known about precise septin expression. Here, we use the well-established model organism zebrafish (Danio rerio) to unravel the expression of sept8a and sept8b, with special focus on the CNS. We performed whole mount RNA in situ hybridization on zebrafish 1-4 dpf in combination with serial sectioning of epon-embedded samples as well as on brain sections of adult zebrafish to obtain precise histological mapping of gene expression...
April 14, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28400174/jmj24-antagonizes-histone-h3k9-demethylase-ibm1-jmj25-function-and-interacts-with-rnai-pathways-for-gene-silencing
#14
Laure Audonnet, Yuan Shen, Dao-Xiu Zhou
Dimethylation of histone H3 lysine 9 (H3K9me2) is a heterochromatic mark linked to DNA methylation and gene repression. Removal of H3K9me2 from gene bodies by the jmjC histone demethylase IBM1/JMJ25 inhibits DNA methylation and derepresses gene expression. In this work, we analyzed the function of a closely related homolog of IBM1/JMJ25, namely JMJ24. We show that jmj24 mutations produced a number of subtle developmental defects, while affecting only a relatively small number of genes at the vegetative stage...
April 8, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28363633/high-resolution-methylation-analysis-of-the-hoxa5-regulatory-region-in-different-somatic-tissues-of-laboratory-mouse-during-development
#15
Puja Sinha, Kiran Singh, Manisha Sachan
Homeobox genes encode a group of DNA binding regulatory proteins whose key function occurs in the spatial-temporal organization of genome during embryonic development and differentiation. The role of these Hox genes during ontogenesis makes it an important model for research. HoxA5 is a member of Hox gene family playing a central role during axial body patterning and morphogenesis. DNA modification studies have shown that the function of Hox genes is partly governed by the methylation-mediated gene expression regulation...
January 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28363632/regional-localization-of-activin-%C3%AE-a-activin-%C3%AE-c-follistatin-proliferation-and-apoptosis-in-adult-and-developing-mouse-prostate-ducts
#16
Elspeth Gold, Sylvia Zellhuber-McMillan, Gail Risbridger, Francesco Elia Marino
Activins and inhibins, members of the TGF-β superfamily, are growth and differentiation factors involved in the regulation of several biological processes, including reproduction, development, and fertility. Previous studies have shown that the activin-βA subunit plays a pivotal role in prostate development. Activin-A inhibits branching morphogenesis in the developing prostate, and its expression is associated with increased apoptosis in the adult prostate. Follistatin, a structurally unrelated protein to activins, is an antagonist of activin-A...
January 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28351515/the-expression-of-sialyltransferases-is-regulated-by-the-bioavailability-and-biosynthesis-of-sialic-acids
#17
Kaya Bork, Wenke Weidemann, Beatrice Berneck, Magdalena Kuchta, Dorit Bennmann, Annett Thate, Otmar Huber, Vinayaga S Gnanapragassam, Rüdiger Horstkorte
Glycosylation is the most frequent and important post-translational modification of proteins. It occurs on specific consensus sequences but the final structure of a particular glycan is not coded on the DNA, rather it depends on the expression of the required enzymes and the availability of substrates (activated monosaccharides). Sialic acid (Sia) is the terminal monosaccharide of most glycoproteins or glycolipids (= glycoconjugates) and involved in a variety of function on molecular (e.g. determination of protein stability and half-life) and cellular level (e...
January 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28300657/expression-patterns-of-prune2-is-regulated-by-notch-and-retinoic-acid-signaling-pathways-in-the-zebrafish-embryogenesis
#18
Mrudhula Anuppalle, Sateesh Maddirevula, Ajeet Kumar, Tae-Lin Huh, Joonho Choe, Myungchull Rhee
PRUNE2 has been identified as a susceptible gene for Alzheimer's disease and a marker for leiomyosarcomas. Isoforms of Prune2 regulate neuronal cell differentiation and synaptogenesis. Although expression pattern of Prune2 has been reported in the murine brain, its expression patterns and regulation along vertebrate embryogenesis remain to be further investigated. We thus defined the expression profiles and transcriptional regulation of prune2 in zebrafish embryos. prune2 exhibits maternal expression, but is increased in later embryonic stages, and expressed in the telencephalon, epiphysis cluster, nucleus of the tract of the post optic commissure, spinal cord, cerebellum, tegmentum, anterior lateral line ganglion, posterior lateral line ganglion and rhombomeres 2 through 5...
January 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28288836/temporal-profiling-of-photoreceptor-lineage-gene-expression-during-murine-retinal-development
#19
Tooka Aavani, Nobuhiko Tachibana, Valerie Wallace, Jeffrey Biernaskie, Carol Schuurmans
Rod and cone photoreceptors are photosensitive cells in the retina that convert light to electrical signals that are transmitted to visual processing centres in the brain. During development, cones and rods are generated from a common pool of multipotent retinal progenitor cells (RPCs) that also give rise to other retinal cell types. Cones and rods differentiate in two distinct waves, peaking in mid-embryogenesis and the early postnatal period, respectively. As RPCs transition from making cones to generating rods, there are changes in the expression profiles of genes involved in photoreceptor cell fate specification and differentiation...
January 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28167138/characterization-of-arteriovenous-identity-in-the-developing-neonate-mouse-retina
#20
Angela M Crist, Chandler Young, Stryder M Meadows
The murine retina has become an ideal model to study blood vessel formation. Blood vessels in the retina undergo various processes, including remodeling and differentiation, to form a stereotypical network that consists of precisely patterned arteries and veins. This model presents a powerful tool for understanding many different aspects of angiogenesis including artery and vein (AV) cell fate acquisition and differentiation. However, characterization of AV differentiation has been largely unexplored in the mouse retinal model...
January 2017: Gene Expression Patterns: GEP
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