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Gene Expression Patterns: GEP

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https://www.readbyqxmd.com/read/29454044/the-expression-patterns-of-vestigial-like-family-member-4-genes-in-zebrafish-embryogenesis
#1
Chang Xue, Hai Hong Wang, Jun Zhu, Jun Zhou
Transcriptional cofactor Vestigial-like 4 (VGLL4) was considered to take part in the early stage of development. Different from human, three paralogs of vgll4 were found in zebrafish, which were vgll4a, vgll4b and vgll4l. However, the expression patterns of the three paralogs during zebrafish development remains unknown. In this study, we used in situ hybridization to elucidate the temporal and spatial expression of zebrafish vgll4 paralogs during normal embryonic and larval development. Similar expression was shown in certain areas at similar stages for the three paralogs...
February 14, 2018: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29427758/screen-for-slit-robo-signaling-in-trunk-neural-cells-reveals-new-players
#2
Darwin Martinez, Nora Zuhdi, Michelle Reyes, Blanca Ortega, Dion Giovannone, Vivian Lee, Maria Elena de Bellard
Slits ligands and their Robo receptors are involved in quite disparate cell signaling pathways that include axon guidance, cell proliferation, cell motility and angiogenesis. Neural crest cells emerge by delamination from neural cells in the dorsal neural tube, and give rise to various components of the peripheral nervous system in vertebrates. It is well established that these cells change from a non-migratory to a highly migratory state allowing them to reach distant regions before they differentiate. However, but the mechanism controlling this delamination and subsequent migration are still not fully understood...
February 7, 2018: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29413438/early-development-of-the-enteric-nervous-system-visualized-by-using-a-new-transgenic-zebrafish-line-harboring-a-regulatory-region-for-choline-acetyltransferase-a-chata-gene
#3
Masataka Nikaido, Saki Izumi, Honoka Ohnuki, Yuki Takigawa, Kyo Yamasu, Kohei Hatta
The enteric nervous system (ENS) is the largest part of the peripheral nervous system in vertebrates. Toward the visualization of the development of the vertebrate ENS, we report our creation of a new transgenic line, Tg(chata:GGFF2) which has a 1.5-kb upstream region of the zebrafish choline acetyltransferase a (chata) gene followed by modified green fluorescent protein (gfp). During development, GFP + cells were detected in the gut by 60 h post-fertilization (hpf). In the gut of 6- and 12-days post-fertilization (dpf) larvae, an average of 92% of the GFP + cells were positive for the neuronal marker HuC/D, suggesting that GFP marks enteric neurons in this transgenic line...
February 2, 2018: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29339137/oxygen-induced-alterations-in-the-expression-of-chromatin-modifying-enzymes-and-the-transcriptional-regulation-of-imprinted-genes
#4
William M Skiles, Avery Kester, Jane H Pryor, Mark E Westhusin, Michael C Golding, Charles R Long
Embryo culture and assisted reproductive technologies have been associated with a disproportionately high number of epigenetic abnormalities in the resulting offspring. However, the mechanisms by which these techniques influence the epigenome remain poorly defined. In this study, we evaluated the capacity of oxygen concentration to influence the transcriptional control of a selection of key enzymes regulating chromatin structure. In mouse embryonic stem cells, oxygen concentrations modulated the transcriptional regulation of the TET family of enzymes, as well as the de novo methyltransferase Dnmt3a...
January 12, 2018: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29277544/cloning-and-gene-expression-analysis-of-two-cdna-of-cysteine-proteinase-genes-involved-in-programmed-cell-death-in-the-inner-integument-from-developing-seeds-of-jatropha-curcas-l
#5
Antônio José Rocha, Simone Pohl, Cristiane Sá Roriz Fonteles
In this paper, two cysteine proteinases were cloned from Jatropha curcas seeds. The full length cDNAs obtained from cloning of Jc-CysEP1 and Jc-CysEP2 genes were 1.516bp and 1500 pb, respectively. The Jc-CysEP1 contained a 1083bp open reading frame (ORF) coding for 360 amino acids. The JcCysEP1 protein sequence had an estimated native molecular weight of 36.89 kDa, with a predicted isoelectric point of 4.55. The average lengths of JcCysEP1 5' UTR and 3' UTR were 269 bp and 167bp, respectively. The Jc-CysEP2 contained a 1077 pb open reading frame (ORF) that encoded 358 amino acids...
December 19, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29247850/tissue-specific-expression-and-in-silico-characterization-of-a-putative-cysteine-synthase-gene-from-lathyrus-sativus-l
#6
Saikat Chakraborty, Joy Mitra, Milan Kumar Samanta, Narattam Sikdar, Jagannath Bhattacharyya, Anulina Manna, Subrata Pradhan, Anirban Chakraborty, Bikas Ranjan Pati
Grass pea (Lathyrus sativus L.) is a worldwide popular pulse crop especially for its protein rich seeds with least production cost. However, the use of the crop became controversial due to the presence of non-protein amino acid, β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP) in its seed and leaf, which is known as the principle neurotoxin to cause neurolathyrism (a motor neurodegenerative disease of humans and animals) during prolonged consumption as regular diet. Till date, the knowledge on β-ODAP biosynthesis in Lathyrus sp...
December 13, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29225067/the-dynamics-of-native-atoh7-protein-expression-during-mouse-retinal-histogenesis-revealed-with-a-new-antibody
#7
Joel B Miesfeld, Tom Glaser, Nadean L Brown
The Atoh7 transcription factor catalyzes the rate-limiting step in the specification of retinal ganglion cells (RGCs). As a tool to study vertebrate retinal development, we validate an antibody that recognizes human and mouse Atoh7 polypeptide, using informative knockout and transgenic mouse tissues and overexpression experiments. The transient features of Atoh7 protein expression during retinal neurogenesis match the expected pattern at the tissue and cellular level. Further, we compare endogenous Atoh7 to established RGC markers, reporter mouse lines and cell cycle markers, demonstrating the utility of the antibody to investigate molecular mechanisms of retinal histogenesis...
December 7, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29183840/comparative-expression-analyses-of-bone-morphogenetic-protein-4-bmp4-expressions-in-muscles-of-tilapia-and-common-carp-indicate-that-bmp4-plays-a-role-in-the-intermuscular-bone-distribution-in-a-dose-dependent-manner
#8
Shengyan Su, Zaijie Dong
Intermuscular bones in fish negatively influence both meet processing and attractiveness to consumers. Tilapia (Oreochromis niloticus) and common carp (Cyprinus carpio) are both major farmed fish species globally, but whereas the former does not possess intermuscular bones, the latter does. Therefore, these two species might present a good model to study the genetic control of distribution of intermuscular bones in fish. Bone morphogenetic protein 4 (BMP4) gene is associated with tissue ossification and bone regeneration in mammals, but in fish its role in ossification remains understudied...
November 25, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29155305/hey1-and-hey2-are-differently-expressed-during-mouse-tooth-development
#9
Kotono Kibe, Mitsushiro Nakatomi, Shinji Kataoka, Takashi Toyono, Yuji Seta
The Hey family (also known as Chf, Herp, Hesr, and Hrt) is a set of Hairy/Enhancer of Split-related basic helix-loop-helix type transcription factors. Hey1, Hey2, and HeyL have been identified in mammals. Although Hey proteins are known to regulate cardiovascular development, muscle homeostasis, osteogenesis, neurogenesis, and oncogenesis, their roles in tooth development have been largely obscure. Therefore, this study aimed to clarify detailed spatiotemporal expression patterns of Hey1 and Hey2 in developing molars and incisors of mice by section in situ hybridization...
November 16, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29155075/asymmetric-distribution-of-crumbs-polarity-complex-proteins-from-compacted-8-cell-to-blastocyst-stage-during-mouse-preimplantation-development
#10
Xinlong Jiang, Wenzhong An, Xiao Yang, Jieye Lin, Shiliang Ma, Dajia Wang, Shuang Tang
During mouse preimplantation development, blastomeres are equipotent until polarity establishment at compacted 8-cell stage. The intrinsic nature of polarity is the asymmetric distribution of polarity proteins between inside and outside blastomeres along the direction of apical-basal axis. This study investigated the early developmental temporal and spatial expression of the main CRUMBS polarity complex proteins in the mouse preimplantation embryo. We observed that Crb3, Pals1, Patj and Mpdz are transcribed in the mouse preimplantation embryo...
November 15, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29128397/identification-and-characterization-of-molluscan-caveolin-1-ortholog-from-haliotis-discus-discus-possible-involvement-in-embryogenesis-and-host-defense-mechanism-against-pathogenic-stress
#11
H M V Udayantha, S D N K Bathige, Thanthrige Thiunuwan Priyathilaka, Sukkyoung Lee, Myoung-Jin Kim, Jehee Lee
Caveolins are principal membrane proteins of caveolae that play a central role in signal transduction, substrate transport, and membrane trafficking in various cell types. Numerous studies have reported the crucial role of caveolin-1 (CAV1) in response to invading microbes; yet, very little is known about molluscan CAV1. In this study, we identified and characterized CAV1 ortholog from the disk abalone, Haliotis discus discus (HdCAV1). The cDNA sequence of HdCAV1 is 826 bp long and encodes a 127-amino acid polypeptide...
November 8, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29126985/spatiotemporal-expression-pattern-of-connexin-43-during-early-chick-embryogenesis
#12
Karyn Jourdeuil, Lisa A Taneyhill
During embryogenesis, a single cell develops into new tissues and organs that are made up of a number of different cell types. The assembly of the trigeminal ganglion (cranial nerve V), an important component of the peripheral nervous system, typifies this process. The trigeminal ganglia perform key sensory functions, including sensing pain and touch in the face, and arise from cells of two different progenitor populations, the neural crest and the cranial placodes. One question that remains poorly understood is how these two populations of cells interact with each other during development to form a functional ganglion...
November 7, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29122676/a-six-gene-expression-toolbox-for-the-glands-epithelium-and-chondrocytes-in-the-mouse-nasal-cavity
#13
Yong Wan, Matthew B Rogers, Heather L Szabo-Rogers
The nose is the central feature of the amniote face. In adults, the nose is a structurally and functionally complex organ that consists of bone, cartilage, glands and ducts. In an ongoing expression screen in our lab, we found several novel markers for specific tissues in the nasal region. Here, using in situ hybridization expression experiments, we report that Alx1, Ap-2β, Crispld1, Eya4, Moxd1, and Penk have tissue specific expression during murine nasal development. At E11.5, we observed that Alx1, Ap-2β, Crispld1, and Eya4 are expressed in the medial and lateral nasal prominences...
November 6, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29122675/high-throughput-method-for-extracting-and-visualizing-the-spatial-gene-expressions-from-in-situ-hybridization-images-a-case-study-of-the-early-development-of-the-sea-anemone-nematostella-vectensis
#14
A M Abdol, Andrew Bedard, Imke Lánský, J A Kaandorp
Studying the spatial gene expression profiles from in situ hybridization images of the embryo is one of the first steps toward the comprehensive understanding of gene interactions in an organism. In the case of N. vectensis, extracting and collecting these data is a challenging task due to the difficulty of detecting the cell layer through the transparent body plan and changing morphology during the blastula and gastrula stages. Here, first, we introduce a method to algorithmically identify and track the cell layer in N...
November 6, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28865954/comparative-expression-profiling-of-atrad5b-and-atndl1-hints-towards-a-role-in-g-protein-mediated-signaling
#15
Nisha Khatri, Swati Singh, Nasmeen Hakim, Yashwanti Mudgil
Arabidopsis AtRAD5B encodes for a putative helicase of the class SWItch/Sucrose Non-Fermentable (SWI/SNF) ATPases. We identified AtRAD5B as an interactor of N-MYC DOWNREGULATED-LIKE1 (AtNDL1) in a yeast two-hybrid screen. AtNDL1 is a G protein signaling component which regulates auxin transport and gradients together with GTP binding protein beta 1 (AGB1). Auxin gradients are known to recruit SWI/SNF remodeling complexes to the chromatin and regulate expression of genes involved in flower and leaf formation...
November 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28843894/modulation-of-gene-expression-by-nutritional-state-and-hormones-in-bombyx-larvae-in-relation-to-its-growth-period
#16
Bembem Thounaojam, Bela Keshan
Insect growth and development are mainly regulated via synchronization of many extrinsic and intrinsic factors such as nutrition and hormones. Previously we have demonstrated that larval growth period influences the effect of insulin on the accumulation of glycogen in the fat body of Bombyx larvae. In the present study we demonstrate that Bombyx larvae at the terminal growth period (TGP, after critical weight) had a significantly greater increase in the expression level of Akt in the fat body than at the active growth period (AGP, before critical weight)...
November 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28826993/expression-and-characterization-of-the-zebrafish-orthologue-of-the-human-folr1-gene-during-embryogenesis
#17
RoJenia N Jones, Stephanie A Erhard, Mark R Malham, Ayaz Y Gen, Kyle Sullivan, Kenneth W Olsen, Rodney M Dale
It has been well established that many types of rapidly dividing normal and diseased cells require an increased amount of folate for DNA replication and repair as well as cellular metabolism. Thus one of folate's cognate receptors, Folate Receptor 1 (FOLR1) is usually up-regulated in rapidly dividing cells, including many types of cancerous tumors. Because zebrafish have become a model organism for understanding conserved vertebrate cellular pathways and human disease, there has been an increased need to identify and elucidate orthologous zebrafish genes that are central to known human maladies...
November 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28800889/spatio-temporal-expression-patterns-of-wnt-signaling-pathway-during-the-development-of-temporomandibular-condylar-cartilage
#18
Kan Chen, Huixin Quan, Gang Chen, Di Xiao
There is a growing body of evidence supporting the involvement of the Wnt signaling pathway in various aspects of skeletal and joint development; however, it is unclear whether it is involved in the process of temporomandibular joint development. In order to clarify this issue, we examined the spatio-temporal distribution of mRNAs and proteins of the Wnt family during the formation of the mandibular condylar cartilage at the prenatal and postnatal stages. An in situ hybridization test revealed no mRNAs of β-catenin and Axin2 during early mesenchymal condensation; the ligands surveyed in this study (including Wnt-4, 5a, and 9a) were clearly detected at various ranges of expression, mainly in the condylar blastema and later distinct cartilaginous layers...
November 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28710028/gene-expression-profiles-of-fin-regeneration-in-loach-paramisgurnus-dabryanu
#19
Li Li, Jingya He, Linlin Wang, Weihua Chen, Zhongjie Chang
Teleost fins can regenerate accurate position-matched structure and function after amputation. However, we still lack systematic transcriptional profiling and methodologies to understand the molecular basis of fin regeneration. After histological analysis, we established a suppression subtraction hybridization library containing 418 distinct sequences expressed differentially during the process of blastema formation and differentiation in caudal fin regeneration. Genome ontology and comparative analysis of differential distribution of our data and the reference zebrafish genome showed notable subcategories, including multi-organism processes, response to stimuli, extracellular matrix, antioxidant activity, and cell junction function...
November 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28669683/effects-of-interferon-tau-on-endometrial-epithelial-cells-in-caprine-in%C3%A2-vitro
#20
Yue Zhang, Lei Zhang, Chaofeng Yu, Xiaoyan Du, Xiaorui Liu, Junze Liu, Xiaopeng An, Jiangang Wang, Yuxuan Song, Guang Li, Binyun Cao
Embryo attachment, a precondition of ruminant pregnancy, has been recognized to be related to apoptosis in endometrial epithelial cells (EECs). In ruminants, interferon tau (IFNT) is secreted by trophoblast of conceptus and works in a concentration-dependent style. To verify the function of IFNT in caprine embryo attachment, caprine EECs were dealt with IFNT at 0, 1, 10 and 100 ng/ml. In this study, IFNT arrested caprine EEC cell cycle in G2 phase and induced cell apoptosis at 1 ng/ml and 10 ng/ml of IFNT...
November 2017: Gene Expression Patterns: GEP
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