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Gene Expression Patterns: GEP

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https://www.readbyqxmd.com/read/28988845/a-bombyx-homolog-of-ovo-is-a-segmentation-gene-that-acts-downstream-of-bm-wnt1-bombyx-wnt1-homolog
#1
Hajime Nakao
Insect embryogenesis is divided into long and short/intermediate germ types. The long germ type may exhibit Drosophila-like hierarchical segmentation mechanisms, whereas the short/intermediate type assumes some repeating mechanisms that are considered to be ancestral. Embryogenesis in Bombyx mori possesses both characteristics. Here, Bombyx ovo homolog (Bm-ovo) was identified as a gene involved in segmentation. Ovo is a Drosophila gene that encodes a zinc finger transcription factor and studies on its homolog functions in other systems have suggested that it acts as a switch to enable the initiation of differentiation from a progenitor cell state...
October 5, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28987646/molecular-cloning-and-expression-of-octamer-binding-transcription-factor-oct4-in-the-large-yellow-croaker-larimichthys-crocea
#2
Yonghua Jiang, Kunhuang Han, Shihai Chen, Mingyi Cai, Yilei Wang, Ziping Zhang
Octamer-binding transcription factor 4 (Oct4) is a crucial pluripotent transcription factor in controlling pluripotency of embryonic stem cells (ESCs), formation of primordial germ cells (PGCs), early embryonic development, and gonadal germ cells. To understand the roles of Oct4 in the large yellow croaker (Larimichthys crocea) (Lc-Oct4), the full-length cDNA of Oct4 was cloned and analyzed. Lc-Oct4 includes a 104-bp 5' untranslated region (UTR), a 567-bp 3' UTR and a 1431-bp coding region encoding a protein of 476 amino acids (aa) with a predicted molecular mass (Mm) of 52...
October 4, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28958789/expression-analyzes-of-early-factors-in-midbrain-differentiation-programs
#3
Simone Mesman, Sonja J Krüse, Marten P Smidt
Mesodiencephalic dopaminergic (mdDA) neurons are born in the ventricular zone (VZ) of the midbrain between E10 and E12. Although these neurons all express specific DA markers like Th and Pitx3, they are subdivided into distinct subsets, each depending on a unique set of transcription factors and signaling cascades for their differentiation. How a neural progenitor commits to an mdDA neuronal cell-fate and how the specification into the different subsets is determined remains unclear. To gain more insight into the development and specification of these neurons we have previously conducted a genome-wide expression analysis, in which dissected midbrain material (E10...
September 27, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28865954/comparative-expression-profiling-of-atrad5b-and-atndl1-hints-towards-a-role-in-g-protein-mediated-signaling
#4
Nisha Khatri, Swati Singh, Nasmeen Hakim, Yashwanti Mudgil
Arabidopsis AtRAD5B encodes for a putative helicase of the class SWItch/Sucrose Non-Fermentable (SWI/SNF) ATPases. We identified AtRAD5B as an interactor of N-MYC DOWNREGULATED-LIKE1 (AtNDL1) in a yeast two-hybrid screen. AtNDL1 is a G protein signaling component which regulates auxin transport and gradients together with GTP binding protein beta 1 (AGB1). Auxin gradients are known to recruit SWI/SNF remodeling complexes to the chromatin and regulate expression of genes involved in flower and leaf formation...
September 1, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28843894/modulation-of-gene-expression-by-nutritional-state-and-hormones-in-bombyx-larvae-in-relation-to-its-growth-period
#5
Bembem Thounaojam, Bela Keshan
Insect growth and development are mainly regulated via synchronization of many extrinsic and intrinsic factors such as nutrition and hormones. Previously we have demonstrated that larval growth period influences the effect of insulin on the accumulation of glycogen in the fat body of Bombyx larvae. In the present study we demonstrate that Bombyx larvae at the terminal growth period (TGP, after critical weight) had a significantly greater increase in the expression level of Akt in the fat body than at the active growth period (AGP, before critical weight)...
August 23, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28826993/expression-and-characterization-of-the-zebrafish-orthologue-of-the-human-folr1-gene-during-embryogenesis
#6
RoJenia N Jones, Stephanie A Erhard, Mark R Malham, Ayaz Y Gen, Kyle Sullivan, Kenneth W Olsen, Rodney M Dale
It has been well established that many types of rapidly dividing normal and diseased cells require an increased amount of folate for DNA replication and repair as well as cellular metabolism. Thus one of folate's cognate receptors, Folate Receptor 1 (FOLR1) is usually up-regulated in rapidly dividing cells, including many types of cancerous tumors. Because zebrafish have become a model organism for understanding conserved vertebrate cellular pathways and human disease, there has been an increased need to identify and elucidate orthologous zebrafish genes that are central to known human maladies...
August 18, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28800889/spatio-temporal-expression-patterns-of-wnt-signaling-pathway-during-the-development-of-temporomandibular-condylar-cartilage
#7
Kan Chen, Huixin Quan, Gang Chen, Di Xiao
There is a growing body of evidence supporting the involvement of the Wnt signaling pathway in various aspects of skeletal and joint development; however, it is unclear whether it is involved in the process of temporomandibular joint development. In order to clarify this issue, we examined the spatio-temporal distribution of mRNAs and proteins of the Wnt family during the formation of the mandibular condylar cartilage at the prenatal and postnatal stages. An in situ hybridization test revealed no mRNAs of β-catenin and Axin2 during early mesenchymal condensation; the ligands surveyed in this study (including Wnt-4, 5a, and 9a) were clearly detected at various ranges of expression, mainly in the condylar blastema and later distinct cartilaginous layers...
August 8, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28710028/gene-expression-profiles-of-fin-regeneration-in-loach-paramisgurnus-dabryanu
#8
Li Li, Jingya He, Linlin Wang, Weihua Chen, Zhongjie Chang
Teleost fins can regenerate accurate position-matched structure and function after amputation. However, we still lack systematic transcriptional profiling and methodologies to understand the molecular basis of fin regeneration. After histological analysis, we established a suppression subtraction hybridization library containing 418 distinct sequences expressed differentially during the process of blastema formation and differentiation in caudal fin regeneration. Genome ontology and comparative analysis of differential distribution of our data and the reference zebrafish genome showed notable subcategories, including multi-organism processes, response to stimuli, extracellular matrix, antioxidant activity, and cell junction function...
July 18, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28669683/effects-of-interferon-tau-on-endometrial-epithelial-cells-in-caprine-in%C3%A2-vitro
#9
Yue Zhang, Lei Zhang, Chaofeng Yu, Xiaoyan Du, Xiaorui Liu, Junze Liu, Xiaopeng An, Jiangang Wang, Yuxuan Song, Guang Li, Binyun Cao
Embryo attachment, a precondition of ruminant pregnancy, has been recognized to be related to apoptosis in endometrial epithelial cells (EECs). In ruminants, interferon tau (IFNT) is secreted by trophoblast of conceptus and works in a concentration-dependent style. To verify the function of IFNT in caprine embryo attachment, caprine EECs were dealt with IFNT at 0, 1, 10 and 100 ng/ml. In this study, IFNT arrested caprine EEC cell cycle in G2 phase and induced cell apoptosis at 1 ng/ml and 10 ng/ml of IFNT...
June 29, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28669682/differential-expression-pattern-of-key-regulatory-developmental-genes-in-pre-implant-zona-free-cloned-vs-in%C3%A2-vitro-fertilized-goat-embryos
#10
Dharmendra Kumar, Bikash Chandra Sarkhel
The success of Somatic cell nuclear transfer (SCNT) primarily depends on the extent of reprogramming of donor cells genome. The error of reprogramming may lead to inappropriate expression of embryonic genes at any stage of development. Under the present study the relative expression of different genes related to pluripotency (Oct-4 and Nanog), growth factors (IGF-2 and IGF-2R) and DNA methyltransferase gene (Dnmt-1) was evaluated in SCNT embryos at 8-16 cells, morula and blastocyst stages as compared to IVF group...
June 29, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28642207/arabidopsis-thaliana-sucrose-phosphate-synthase-sps-genes-are-expressed-differentially-in-organs-and-tissues-and-their-transcription-is-regulated-by-osmotic-stress
#11
María Gloria Solís-Guzmán, Gerardo Argüello-Astorga, José López-Bucio, León Francisco Ruiz-Herrera, Joel Edmundo López-Meza, Lenin Sánchez-Calderón, Yazmín Carreón-Abud, Miguel Martínez-Trujillo
Sucrose is synthesized from UDP-Glc and Fru-6-phosphate via the activity of sucrose-phosphate synthase (SPS) enzymes, which produce Suc-6-phosphate. Suc-6-phosphate is rapidly dephosphorylated by phosphatases to produce Suc and inorganic phosphate. Arabidopsis has four sps genes encoding SPS enzymes. Of these enzymes, AtSPS1F and AtSPS2F have been grouped with other dicotyledonous SPS enzymes, while AtSPS3F and AtSPS4F are included in groups with both dicotyledonous and monocotyledonous SPS enzymes. In this work, we generated Arabidopsis thaliana transformants containing the promoter region of each sps gene fused to gfp::uidA reporter genes...
June 20, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28634012/different-expression-of-sox17-gene-during-gametogenesis-between-scallop-chlamys-farreri-and-vertebrates
#12
Shaoshuai Liang, Zhifeng Zhang, Dandan Yang, Yingying Chen, Zhenkui Qin
SOX17, a member of SRY-related high-mobility-group box (SOX) family, involves in endoderm formation, angiogenesis and carcinogenesis, and its expression characteristics are different in spermatogenesis among several vertebrates. In this study, we cloned a full-length cDNA sequence of sox17 from Zhikong scallop Chlamys farreri, and determined its expression characteristics in gonad at mRNA and protein levels. The cDNA sequence was 2802 bp in length, predicted to encode a protein of 511 amino acids and contained a conservative HMG-box of SOX family, while lacked the C-terminal region of SOX17 comparing to vertebrates...
June 17, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28629960/molecular-cloning-and-mrna-expression-pattern-of-sox4-in-paramisgurnus-dabryanus
#13
Xiaohua Xia, Ruyan Wan, Weiran Huo, Linxia Zhang, Xiaopei Xia, Zhongjie Chang
Sox4 belonged to the SoxC subfamily of the Sox family, which play important roles in the development of the vertebrate gonad and nervous system. A Sox4 homologue was cloned from brain of Paramisgurnus dabryanus by using homologous cloning and rapid amplification of cDNA ends (RACE), designated as PdSox4. The full-length cDNA was 2163bp, containing the 759bp 5'-untranslated region, 267bp 3'-untranslated region and encoding a putative protein of 378 amino acids with a characteristic high mobility group box (HMG-box) DNA-binding domain of 79 amino acids with the specific motif (RPMNAFMVW)...
June 16, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28625897/differential-expression-of-long-noncoding-rnas-in-congenital-microtia
#14
Ling Zhang, Lin Lin, Yu-Peng Song, Bo Pan, Qing-Hua Yang, Hai-Yue Jiang
OBJECTIVE: To analyse lncRNA expression profiles in microtia using bioinformatics analysis. METHODS: We examined lncRNA expression profiles in residual ear cartilage and normal ear cartilage from individual congenital microtia patients. RESULTS: The gene chips used in this study included 30586 lncRNAs and 26109 mRNA probes. Intotal, 180 lncRNAs with differential expression weredetected in the residual ear cartilage compared with the normal cartilage, including 74 up-regulated and 106down-regulated lncRNAs...
June 15, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28625896/lateralization-of-housekeeping-genes-in-the-brain-of-one-day-old-chicks
#15
Jacek Lenart, Klaudia Kogut, Elzbieta Salinska
Real-time quantitative PCR is an exceptionally sensitive method that can detect even very small differences in gene expression and, as such, it is essential to use suitable reference genes. Domestic chickens are used in a wide range of studies including neurobiology, behavior, ecology and disease transmission. In recent avian gene expression experiments, 18S (18S ribosomal RNA), beta actin (ACTB) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) have frequently been used; however, there is not enough evidence that these reference genes are suitable for all types of experiments...
June 15, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28625895/expression-analyses-of-salinity-stress-associated-ests-in-aeluropus-littoralis
#16
Tayebeh Kakeshpour, Sajad Rashidi Monfared, Amin Ebrahimi, Arman Beyraghdar Kashkooli, Esmaeil Ebrahimie
Salinity is among the most important abiotic stresses affecting crop production throughout the earth. Halophyte plants can sustain high salinity levels, therefore elucidating molecular mechanisms underlying their salinity resistance is beneficial for crop improvement. Aeluropus littoralis, a halophyte weed, is a great genetic resource for this purpose. Isolated expressed sequence taq (EST) sequences from A. littoralis under salinity stress, have given us the chance to find and analyze transcripts of genes involved in response to salinity...
June 15, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28624368/conservation-of-bmp2-4-expression-patterns-within-the-clade-branchiostoma-amphioxus-resolving-interspecific-discrepancies
#17
Luok Wen Yong, Stéphanie Bertrand, Jr-Kai Yu, Hector Escriva, Nicholas D Holland
In 2016, Kaji et al. concluded that the amphioxus mouth has the quality of a coelomoduct and is, therefore, not homologous to the oral opening of any other animal. They studied a Japanese population of Branchiostoma japonicum and based their conclusion, in part, on the larval expression of BMP2/4 in cells that reportedly joined the rim of the forming mouth. They did not detect transcription of that gene in any other tissues in the anterior region of the larva. Their results were almost the inverse of findings for B...
June 15, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28610924/embryonic-expression-patterns-of-eukaryotic-endou-ribonuclease-family-gene-endouc-in-zebrafish
#18
Hung-Chieh Lee, Chuan-Yang Fu, Chih-Wei Zeng, Huai-Jen Tsai
Endou proteins belong to the Eukaryotic EndoU ribonuclease family of enzymes that present high sequence homology with the founding member XendoU domain. The enzymatic activity and three-dimensional structure of some Endou proteins have been previously reported. However, their molecular structure and gene expression patterns during embryogenesis remain to be elucidated. Therefore, we took zebrafish (Danio rerio) endouC as the model to study molecular structure and gene expression dynamics at different developmental stages...
June 10, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28603023/deciphering-the-role-of-microrna-a-step-by-step-guide
#19
Siddharth Manvati, Kailash Chandra Mangalhara, Juveria Khan, Geeta Lal Pathania, Srishti Kaul, Monika Kaushik, Ankita Arora, Pawan K Dhar
MicroRNAs (miRNAs), are small non-coding RNAs of approximately 22 nucleotides in length, playing an important role in regulating gene expression post-transcriptionally. Understanding the effect of miRNA regulation in a pathway-specific manner unravels the approaches adopted to apprehend biological mechanisms, the information, which is scanty for researchers, not primed already for miR related research. Here, we describe a quick perspective in 5 steps with probable approaches and assays at every level to unravel the specific role of a microRNA, miR-145a-5p, as an example...
June 8, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28583421/gene-expression-profiles-in-mouse-cumulus-cells-derived-from-in%C3%A2-vitro-matured-oocytes-with-and-without-blastocyst-formation
#20
Yixin Xu, Tuanping Zhou, Li Shao, Bei Zhang, Kailu Liu, Chao Gao, Li Gao, Jiayin Liu, Yugui Cui, Ri-Cheng Chian
Cumulus cells (CCs) are considered as an important source to predict oocyte quality. Despite numerous candidate genes in CCs have been identified for embryonic developmental competence, the results are inconsistent. The next generation RNA-sequencing was used to investigate the transcriptomic differences in CCs from in vitro matured oocytes did or did not develop to blastocyst stage following in vitro fertilization (IVF). In our study, the corresponding mouse oocytes were traced using a single-cell tracking system, and CCs were pooled into groups based on the embryonic developmental outcomes...
June 2, 2017: Gene Expression Patterns: GEP
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