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Gene Expression Patterns: GEP

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https://www.readbyqxmd.com/read/27826126/the-expression-patterns-of-tetratricopeptide-repeat-domain-36-ttc36
#1
Yuru Zhou, Qingling He, Jihui Chen, Yunhong Liu, Zhaomin Mao, Zhongshi Lyu, Dongsheng Ni, Yaoshui Long, Pan Ju, Jianing Liu, Yuping Gu, Qin Zhou
Tetratricopeptide repeat domain 36 (Ttc36), whose coding protein belongs to tetratricopeptide repeat (TPR) motif family, has not been studied extensively. We for the first time showed that Ttc36 is evolutionarily conserved across mammals by bioinformatics. Rabbit anti-mouse Ttc36 polyclonal antibody was generated by injecting synthetic full-length peptides through "antigen intersection" strategy. Subsequently, we characterized Ttc36 expression profile in mouse, showing its expression in liver and kidney both from embryonic day 15...
November 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27818290/developmental-expression-of-mglu2-and-mglu3-in-the-mouse-brain
#2
Caitlin E McOmish, Elena Y Demireva, Jay A Gingrich
The glutamatergic system directs central nervous system (CNS) neuronal activity and may underlie various neuropsychiatric disorders. Glutamate transmits its effects through multiple receptor classes. Class II metabotropic glutamate receptors, mGlu2 and mGlu3, play an important role in regulating synaptic release of different neurotransmitter systems and consequently modulate signaling across several neuronal subtypes. Drugs targeting mGlu2 and mGlu3 are seen as potential therapeutics for various psychiatric and neurological disorders, and defining their expression through development can aid in understanding their distinct function...
November 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27670931/expression-of-the-insulinoma-associated-1-insm1-gene-in-xenopus-laevis-tadpole-retina-and-brain
#3
Jennifer L Bosse, Heithem M El-Hodiri
The insulinoma-associated 1 (insm1) gene is involved in the differentiation of several neuronal and endoderm derived cell types. insm1 is expressed in the retina and brain of several vertebrates including Xenopus laevis. We report the detailed expression pattern of insm1 in the X. laevis tadpole retina and brain. X. laevis insm1 is expressed in most of the ciliary marginal zone of the mature retina and the optic tectum, dorsal pallium, hypothalamus and preoptic areas of the developing tadpole brain. Overall, insm1 is expressed in regions of the tadpole brain and retina harboring populations of progenitor cells...
September 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27664768/gene-expression-analysis-during-the-induction-and-patterning-of-the-conjunctival-papillae-in-the-chick-embryonic-eye
#4
Karyn Jourdeuil, Tamara Anne Franz-Odendaal
The induction and patterning of the conjunctival papillae (i.e. epithelial thickenings of the conjunctiva required for the induction of the underlying, neural crest-derived scleral ossicles) is complex. It takes place over a period of two days and follows a defined spatiotemporal sequence. In this study, we investigated the spatial and temporal expression pattern of four genes over seven morphological stages of development of these papillae. We show that β-catenin is expressed during the pre-patterning of the epithelium prior to papilla induction and second that β-catenin, Ednrb and Inhba are expressed during the induction and patterning of the conjunctival papillae...
September 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27613600/expression-profiles-of-the-g%C3%AE-subunits-during-xenopus-tropicalis-embryonic-development
#5
Jaime Fuentealba, Gabriela Toro-Tapia, Marion Rodriguez, Cecilia Arriagada, Alejandro Maureira, Andrea Beyer, Soraya Villaseca, Juan I Leal, Maria V Hinrichs, Juan Olate, Teresa Caprile, Marcela Torrejón
Heterotrimeric G protein signaling plays major roles during different cellular events. However, there is a limited understanding of the molecular mechanisms underlying G protein control during embryogenesis. G proteins are highly conserved and can be grouped into four subfamilies according to sequence homology and function. To further studies on G protein function during embryogenesis, the present analysis identified four Gα subunits representative of the different subfamilies and determined their spatiotemporal expression patterns during Xenopus tropicalis embryogenesis...
September 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27613599/embryonic-expression-patterns-of-hox-genes-in-the-oligochaete-annelid-tubifex-tubifex
#6
Mao Endo, Chiharu Sakai, Takashi Shimizu
We have cloned and characterized the expression of seven Hox genes (designated Ttu-lab, Ttu-Dfd, Ttu-Scr1, Ttu-Scr2, Ttu-Lox5, Ttu-Lox4 and Ttu-Lox2) from the oligochaete annelid Tubifex tubifex. RT-PCR analyses show that except for Ttu-Lox4 and Ttu-Lox2 which begin expression as early as cleavage stages, Tubifex Hox genes are expressed during stages 13-18 when embryos undergo germ band formation, segmentation and body elongation. In terms of combination of tissues (or organs) exhibiting positive cells, the Tubifex Hox genes examined in this study are classified into three groups...
September 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27593944/development-of-the-cardiac-conduction-system-in-zebrafish
#7
Kar-Lai Poon, Michael Liebling, Igor Kondrychyn, Thomas Brand, Vladimir Korzh
The cardiac conduction system (CCS) propagates and coordinates the electrical excitation that originates from the pacemaker cells, throughout the heart, resulting in rhythmic heartbeat. Its defects result in life-threatening arrhythmias and sudden cardiac death. Understanding of the factors involved in the formation and function of the CCS remains incomplete. By transposon assisted transgenesis, we have developed enhancer trap (ET) lines of zebrafish that express fluorescent protein in the pacemaker cells at the sino-atrial node (SAN) and the atrio-ventricular region (AVR), termed CCS transgenics...
July 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27545450/differential-gene-expression-patterns-and-colocalization-of-atp-gated-p2x6-p2x4-ion-channels-during-rat-small-intestine-ontogeny
#8
Karla Padilla, David Gonzalez-Mendoza, Laura C Berumen, Jesica E Escobar, Ricardo Miledi, Guadulupe García-Alcocer
Gene coding for ATP-gated receptor ion channels (P2X1-7) has been associated with the developmental process in various tissues; among these ion channel subtypes, P2X6 acts as a physiological regulator of P2X4 receptor functions when the two receptors form heteroreceptors. The P2X4 receptor is involved in pain sensation, the inflammatory process, and body homeostasis by means of Mg(2+) absorption through the intestine. The small intestine is responsible for the absorption and digestion of nutrients; throughout its development, several gene expressions are induced that are related to nutrients received, metabolism, and other intestine functions...
July 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27521061/expression-patterns-of-homeobox-genes-in-the-mouse-vomeronasal-organ-at-postnatal-stages
#9
Isabelle Chang, Marta Parrilla
Homeodomain proteins are encoded by homeobox genes and regulate development and differentiation in many neuronal systems. The mouse vomeronasal organ (VNO) generates in situ mature chemosensory neurons from stem cells. The roles of homeodomain proteins in neuronal differentiation in the VNO are poorly understood. Here we have characterized the expression patterns of 28 homeobox genes in the VNO of C57BL/6 mice at postnatal stages using multicolor fluorescent in situ hybridization. We identified 11 homeobox genes (Dlx3, Dlx4, Emx2, Lhx2, Meis1, Pbx3, Pknox2, Pou6f1, Tshz2, Zhx1, Zhx3) that were expressed exclusively in neurons; 4 homeobox genes (Pax6, Six1, Tgif1, Zfhx3) that were expressed in all non-neuronal cell populations, with Pax6, Six1 and Tgif1 also expressed in some neuronal progenitors and precursors; 12 homeobox genes (Adnp, Cux1, Dlx5, Dlx6, Meis2, Pbx2, Pknox1, Pou2f1, Satb1, Tshz1, Tshz3, Zhx2) with expression in both neuronal and non-neuronal cell populations; and one homeobox gene (Hopx) that was exclusively expressed in the non-sensory epithelium...
July 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27476414/expression-of-hox-paralog-group-13-genes-in-adult-and-developing-megalobrama-amblycephala
#10
Ivan Jakovlić, Wei-Min Wang
Hox genes encode transcription factors that play a key role in specifying the body plan in most metazoans. HoxPG13 genes most probably played an important role in body length variation during the evolution of animals. This is the first report of the mRNA expression patterns of the entire Hox paralog group 13 (HoxA13a, HoxA13b, HoxB13a, HoxC13a, HoxC13b, HoxD13a) in fish. Expression was studied by qPCR in five tissues of adult Megalobrama amblycephala specimens (spleen, liver, kidney, intestine and gills) and during development (17 stages: fertilised egg to 90 days-old juveniles)...
July 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27241892/spatio-temporal-expression-of-sox-genes-in-murine-palatogenesis
#11
Momoko Watanabe, Katsushige Kawasaki, Maiko Kawasaki, Thantrira Portaveetus, Shelly Oommen, James Blackburn, Takahiro Nagai, Atsushi Kitamura, Atsushi Nishikawa, Yasumitsu Kodama, Ritsuo Takagi, Takeyasu Maeda, Paul T Sharpe, Atsushi Ohazama
Members of the Sox gene family play critical roles in many biological processes including organogenesis. We carried out comparative in situ hybridisation analysis of seventeen Sox genes (Sox1-14, 17, 18 and 21) during murine palatogenesis from initiation to fusion of the palatal shelves above the dorsal side of the tongue. At palatal shelf initiation (E12.5), the localized expression of six Sox genes (Sox2, 5, 6, 9, 12 and 13) was observed in the shelves, whereas Sox4 and Sox11 showed ubiquitious expression...
July 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27221232/first-trimester-trophoblasts-forming-endothelial-like-tubes-in%C3%A2-vitro-emulate-a-blood-vessel-development-gene-expression-profile
#12
Amanda R Highet, Sam Buckberry, Benjamin T Mayne, Sultana M Khoda, Tina Bianco-Miotto, Claire T Roberts
Extravillous cytotrophoblasts isolated from first trimester placenta, and immortalised cell lines derived from them, have the intrinsic ability to form endothelial-like tubes when cultured on Matrigel™ extracellular matrix. This in vitro tube formation may model placental angiogenesis and/or endovascular differentiation by trophoblasts. To interpret the relevance of this phenomenon to placental development, we used a gene expression microarray approach to identify which genes and pathways are associated with the tube-forming phenotype of HTR8/SVneo first trimester trophoblasts (HTR8-M), compared with HTR8/SVneo not forming tubes on plastic culture surface (HTR8-P)...
July 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27063263/expression-of-prolyl-hydroxylases-2-and-3-in-chick-embryos
#13
Jake Barber, Nobue Itasaki
Hypoxic cellular response is crucial for normal development as well as in pathological conditions in order to tolerate low oxygen. The response is mediated by Hypoxia Inducible Factors (HIFs), where the α-subunit of HIF is stabilised and able to function only in low oxygen. Prolyl hydroxylases (PHDs) are oxygen dependent dioxygenase enzymes that hydroxylate HIF-α leading to HIF degradation. Thus PHDs function as an oxygen sensor for the function of HIFs. Here we describe the mRNA expression pattern of PHDs in chick embryos...
July 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27378315/analysis-of-anthocyanin-biosynthesis-genes-expression-profiles-in-contrasting-cultivars-of-japanese-plum-prunus-salicina-l-during-fruit-development
#14
Máximo González, Erika Salazar, Soledad Cabrera, Pilar Olea, Basilio Carrasco
Flavonoids are responsible of different fruit sensorial properties. In Japanese plum (Prunus salicina L.) these compounds are variable in both type and quantity during the different stages of fruit growth and maturation. Here we present the first study which determines the expression profile of structural genes of the flavonoid pathway and accumulation profiles of total phenols, proanthocyanidins and anthocyanins during fruit development stages in contrasting cultivars in Japanese plum. The biosynthesis of these compounds is differentially regulated in different tissues and cultivars...
May 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27364471/kr%C3%A3-pple-like-factors-7-and-6a-mrna-expression-in-adult-zebrafish-central-nervous-system
#15
Sunil Bhattarai, Alicja Sochacka-Marlowe, Gerald Crutchfield, Ramisha Khan, Richard Londraville, Qin Liu
Krüpple-like factors (KLFs) are transcription factors with zinc finger DNA binding domains known to play important roles in brain development and central nervous system (CNS) regeneration. There is little information on KLFs expression in adult vertebrate CNS. In this study, we used in situ hybridization to examine Klf7 mRNA (klf7) and Klf6a mRNA (klf6a) expression in adult zebrafish CNS. Both klfs exhibit wide and similar expression in the zebrafish CNS. Brain areas containing strongly labeled cells include the ventricular regions of the dorsomedial telencephalon, the ventromedial telencephalon, periventricular regions of the thalamus and hypothalamus, torus longitudinalis, stratum periventriculare of the optic tectum, granular regions of the cerebellar body and valvula, and superficial layers of the facial and vagal lobes...
May 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27346542/wnt-frizzled-and-sfrp-gene-expression-patterns-during-gastrulation-in-the-starfish-patiria-asterina-pectinifera
#16
Narudo Kawai, Ritsu Kuraishi, Hiroyuki Kaneko
By the initial phase of gastrulation, Wnt pathway regulation mediates endomesoderm specification and establishes the animal-vegetal axis, thereby leading to proper gastrulation in starfish. To provide insight into the ancestral mechanism regulating deuterostome gastrulation, we identified the gene expression patterns of Wnt, Frizzled (Fz), and secreted frizzled-related protein (sFRP) family genes, which play a role in the initial stage of the Wnt pathway, in starfish Patiria (Asterina) pectinifera embryos using whole mount in situ hybridization...
May 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27289075/an-in-situ-hybridization-study-of-hyaluronan-synthase-has-mrna-in-developing-mouse-molar-and-incisor-tooth-germs
#17
Tsuyoshi Morita, Kaoru Fujikawa, Otto Baba, Shunichi Shibata
Hyaluronan (HA) is a major constituent molecule in most extracellular matrices and is synthesized by Hyaluronan synthase (Has). In the present study, we examined expression patterns of Has1, -2, -3 mRNA in developing mouse molar and incisor tooth germs from embryonic day (E) 11.5 to postnatal day (P) 7, focusing on Hertwig's epithelial root sheath (HERS) and the apical bud in particular. Has1 mRNA expression was not detected in all tooth germs examined. Has2 mRNA was expressed in the surrounding mesenchyme from E12...
May 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27264560/spatiotemporal-expression-pattern-of-the-zebrafish-aquaporin-8-family-during-early-developmental-stages
#18
Soonil Koun, Jun-Dae Kim, Myungchull Rhee, Myoung-Jin Kim, Tae-Lin Huh
Aquaporin 8 (Aqp8) is a transmembrane protein that is selectively permeated by water and some small solutes, and physiologically contributes to acid-base equilibrium in the gastrointestinal tract. Here, we described the characterization and spatiotemporal expression pattern of zebrafish aqp8 (zaqp8) gene family, including zaqp8a.1, zaqp8a.2, and zaqp8b, during the early developmental stages. The expression of zaqp8a.1 started first in the lateral plate mesoderm at the 12-somite stage (ss) and then expanded sequentially to the dorsal aorta, intersegmental blood vessels and then to the dorsal longitudinal anastomotic vessel at 24 h post fertilization (hpf)...
May 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27261260/molecular-characterization-and-developmental-expression-patterns-of-apolipoprotein-a-i-in-senegalese-sole-solea-senegalensis-kaup
#19
J Román-Padilla, A Rodríguez-Rúa, M Manchado, I Hachero-Cruzado
The apolipoprotein A-I (ApoA-I) is an essential component of the high density lipoproteins (HDL). In this study, the cDNA and genomic sequences of this apolipoprotein were characterized for first time in Solea senegalensis. The predicted polypeptide revealed conserved structural features including ten repeats in the lipid-binding domain and some residues involved in cholesterol interaction and binding. The gene structure analysis identified four exons and three introns. Moreover, the synteny analysis revealed that apoA-I did not localize with other apolipoproteins indicating a divergent evolution with respect to the apoA-IV and apoE cluster...
May 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/26994494/new-insights-into-the-role-of-the-brdt-protein-in-the-regulation-of-development-and-spermatogenesis-in-the-mouse
#20
Shimi Barda, Leah Yogev, Gedalia Paz, Haim Yavetz, Ron Hauser, Haim Breitbart, Sandra E Kleiman
The bromodomain testis-specific (BRDT) protein belongs to the bromodomain extra-terminal (BET) family of proteins. It serves as a transcriptional regulator of gene expression during spermatogenesis, and is an essential factor for the normal spermatogenesis process. In this study, we characterized mice of several age groups who lacked the Brdt gene. The testes of Brdt mutant mice aged 8 weeks exhibited complete spermatocyte maturation arrest with a significantly increased number of apoptotic cells. The weights of the testes and accessory glands as well as the testosterone levels of the mutant mice were significantly lower compared to the normal mice...
March 2016: Gene Expression Patterns: GEP
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