journal
MENU ▼
Read by QxMD icon Read
search

Reproductive Biology

journal
https://www.readbyqxmd.com/read/28624353/expression-of-hypoxia-inducible-factor-1%C3%AE-and-antioxidant-enzymes-superoxide-dismutases-1-and-2-in-ischemic-porcine-endometrium
#1
Joanna Kwiatkowska, Barbara Wąsowska, Przemysław Gilun
The aim of this study was to determine how 60-min ischemia changes the expression of hypoxia inducible factor 1α (HIF-1α) and superoxide dismutases (SOD)-1 and -2 in selected regions of porcine uterine horns. The results showed that 60-min ischemia of the porcine uterus conducted at the mid-secretory estrous phase caused decreased HIF-1α and increased SOD-2 gene expression. Higher expression of SOD-2 suggests that this enzyme may play an important role in the suppression of HIF-1α accumulation in an ischemic endometrium...
June 14, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28579338/serine-like-proteolytic-enzymes-from-common-carp-cyprinus-carpio-l-seminal-plasma-are-able-to-degrade-sperm-proteins
#2
Beata Irena Cejko, Mariola Słowińska, Sylwia Judycka, Radosław Kajetan Kowalski
The application of zymography, with sperm proteins as a substrate, allowed for the first time the visualisation of two serine proteinases with a molecular weight of 76 and 163kDa from common carp Cyprinus carpio L. seminal plasma. Twenty four hours of incubation in a development solution with a pH of 7.5 and incubation at 37°C were the best conditions for the visualisation of serine proteinase; however, proteolysis was also observed at 4°C. Our results indicate that serine proteinase from common carp seminal plasma with a molecular weight of 76 and 163kDa may be involved in the degradative mechanism of sperm proteins...
June 1, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28579337/cryopreservation-of-canine-sperm-using-egg-yolk-and-soy-bean-based-extenders
#3
María Jesús Sánchez-Calabuig, Verónica Maillo, Paula Beltrán-Breña, Julio de la Fuente Martínez, Silvestre Galera-Carrillo, José Félix Pérez-Gutiérrez, Serafín Pérez-Cerezales
Animal protein-based extenders are widely used despite being a potential source of bacterial or mycoplasma contamination. Its replacement with vegetal protein-based extenders could represent an interesting alternative for dog sperm cryopreservation. This technique could be further improved by the addition of Tris-Glucose-Citric acid (TGC) that could physically protect the spermatozoa and improve its homeostasis. The aim of this study was to evaluate a cryopreservation protocol for dog spermatozoa using a soybean-based extender (LP1(℗)) as well as the effects of the addition of (TGC) immediately after the semen collection...
May 31, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28576621/chelating-agents-in-combination-with-rosmarinic-acid-for-boar-sperm-freeze-drying
#4
Maite Olaciregui, Victoria Luño, Noelia González, Paula Domingo, Ignacio de Blas, Lydia Gil
The presence of DNA protective agents in the medium is necessary to maintain sperm functionality after freeze-drying procedure. The objective of this study was to investigate the effect of chelating agents, ethylene diaminetetraacetic acid (EDTA) and ethylene glycoltetraacetic acid (EGTA), in combination with rosmarinic acid (RA) on DNA integrity of freeze-dried boar sperm. We also examined the effect of these agents on the in vitro developmental ability of porcine oocytes following sperm injection (ICSI). Heterospermic mix, obtained from ejaculated sperm of three boars, was freeze-dried in two different chelating agents' media: 50mM EDTA or 50mM EGTA, and in these media supplemented with 105μM of rosmarinic acid...
May 30, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28571680/reproductive-effects-of-irisin-initial-in-vitro-studies
#5
Leonid Poretsky, Julie Islam, Dimiter Avtanski, Yu Kuei Lin, Yi-Ling Shen, Yael Hirth, Martin Lesser, Zev Rosenwaks, Donna Seto-Young
The recently discovered myo- and adipokine irisin affects insulin sensitivity in classical insulin target tissues (adipose tissue, skeletal muscle and liver), but the reproductive effects of this hormone, if any, remain largely unexplored. We hypothesized that irisin may have effects on the hypothalamic-pituitary-gonadal axis. To test this hypothesis, we used murine pituitary mPit12 and human ovarian granulosa cells. GnRH treatment resulted in significant (up to 2.5-fold, p<0.0005) and dose-dependent stimulation of LH production by the mPit12 cells...
May 29, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28566207/aberrations-in-sperm-dna-methylation-patterns-are-associated-with-abnormalities-in-semen-parameters-of-subfertile-males
#6
M Laqqan, E F Solomayer, M Hammadeh
Infertility affects about 15% of couples worldwide, with approximately 7% of males suffering from infertility problems. This study was designed to assess the relationship between alterations in sperm DNA methylation patterns and semen parameters in subfertile males. Of a total of 108 males, 30 samples were subjected to 450K BeadChip arrays to evaluate the variation in DNA methylation level between cases and controls. Three CpG sites showed the highest difference in methylation levels (cg09737095, cg14271023, and cg17662493), which are located in the KCNJ5, MLPH, and SMC1β genes, respectively; these were selected for further analysis using deep bisulfite sequencing in 78 independent samples (21 proven fertile "controls", and 57 subfertile "cases")...
May 26, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28552376/involvement-of-stat1-in-proliferation-and-invasiveness-of-trophoblastic-cells
#7
Francisco Lázaro Pereira de Sousa, Wittaya Chaiwangyen, Diana M Morales-Prieto, Stephanie Ospina-Prieto, Maja Weber, Stella M Photini, Nelson Sass, Silvia Daher, Ekkehard Schleussner, Udo R Markert
Trophoblast proliferation and invasion are controlled by cytokines and growth factors present at the implantation site. Members of the Interleukin-6 (IL-6) family of cytokines trigger their effects through activation of intracellular cascades including the Janus Kinase/Signal Transducer and Activator of Transcription (JAK-STAT) pathway. Functions of several STAT molecules in trophoblast cells have been described, but the role of STAT1 remained unclear. Here, potential functions of STAT1 and its activation by Oncostatin M (OSM) have been investigated in an in vitro model...
May 25, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28552375/multivitamins-preventive-therapy-against-subclinical-endometritis-in-buffaloes-its-correlation-to-nefa-and-oxidative-stress
#8
Eman A Ahmed, Doaa H Elsayed, Omnia E Kilany, Marwa A El-Beltagy
The current study was designed to elucidate the in vivo antioxidant, preventive, and ameliorating effects of vitamins AD3E on the incidence of subclinical endometritis (SCE) in buffaloes. Twenty-four buffaloes were divided equally into two groups; group I: control and group II: received AD3E combination. Endometrial cytological samples (n=48) were collected using cytobrush to diagnose SCE by counting polymorphonuclear cells (PMN) ≥6% at 5(th) (W5) and≥4% at 7(th) (W7) weeks postpartum. Results revealed that serum superoxide dismutase and nitric oxide significantly increased and decreased, respectively at W7 in AD3E group...
May 25, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28551031/non-esterified-fatty-acids-in-early-luteal-bovine-oviduct-fluid-mirror-plasma-concentrations-an-ex-vivo-approach
#9
Lies Jordaens, Veerle Van Hoeck, Jessie De Bie, Mario Berth, Waleed F A Marei, Karolien L J Desmet, Peter E J Bols, Jo L M R Leroy
We investigated whether and to which extent plasma non-esterified fatty acids (NEFAs) are reflected in oviduct fluid (OF) using an improved ex vivo flushing method. OF and plasma NEFA concentrations were respectively 0.29±0.19 and 0.31±0.14mmol/L, they didn't differ significantly (P=0.13) and tended to be positively correlated (Pearson correlation coefficient=0.56; P=0.07). Results suggest that OF NEFAs mirror the concentrations seen in plasma of healthy cattle.
May 24, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28546031/bovine-herpes-virus-type-4-alters-tnf-%C3%AE-and-il-8-profiles-and-impairs-the-survival-of-bovine-endometrial-epithelial-cells
#10
Metasu Chanrot, Gunilla Blomqvist, Yongzhi Guo, Karin Ullman, Mikael Juremalm, Renee Bage, Gaetano Donofrio, Jean-Francois Valarcher, Patrice Humblot
Bovine herpes virus type 4 (BoHV-4) can be transmitted by contaminated semen to cows at the time of breeding and may cause uterine disease. The aim of this study was to characterize the susceptibility of bovine endometrial epithelial cells (bEEC) to BoHV-4 by using an in vitro model. When bEEC were challenged with different multiplicity of infection (MOI; from 0.001 to 10) of BoHV-4 for 6days, a significant decrease in cell survival with increasing MOI was observed. The bEEC were subsequently challenged with BoHV-4 MOI 0...
May 22, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28532595/impact-of-elemental-iron-on-human-spermatozoa-and-mouse-embryonic-development-in-a-defined-synthetic-culture-medium
#11
Safaa M Naes, Oshini Basri, Fauziah Ismail, Ghofraan A Ata'Allah, Siti Khadijah Idris, Noor Azmi Mat Adenan, Jaffar Ali
There is a paucity of studies on effect of iron in embryo culture procedures. This study aims to ascertain the optimal, tolerance and toxic levels of iron in a protein-free embryo culture medium (PFM) to determine the effect of iron on embryonic development. The application of PFM in assisted reproductive technologies (ART) is intended to eliminate disease transmission and improve ART treatment outcome. The optimal, tolerance and toxic levels of iron on human spermatozoa and mouse embryos were determined by challenging them with different levels of iron (ferric iron; Fe(+3))...
May 19, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28532594/the-endocrine-disrupting-alkylphenols-and-4-4-ddt-interfere-with-estrogen-conversion-and-clearance-by-mouse-liver-cytosol
#12
Talal El-Hefnawy, Claudia Hernandez, Laura P Stabile
Endocrine disrupting chemicals (EDCs) are ubiquitous compounds known for negative impacts on reproductive functions and for increasing cancer risk. EDCs are believed to cause the harmful effects in part through their inappropriate low-affinity binding to steroid receptors and other possible non-receptor mediated paradigms, however there is a need to further elucidate other mechanisms involving the direct and indirect impact of EDCs on reproductive functions. We examined the metabolism of 17β-estradiol (E2) and estrone (E1) by cell-free hepatic cytosol in the presence of alkylphenols (nonylphenol/NP and 4-tert-octylphenol/tOP), Dichlorodiphenyltrichloroethane (4,4'-DDT) and other EDCs...
May 19, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28527625/cosmetic-micromanipulation-of-vitrified-warmed-cleavage-stage-embryos-does-not-improve-art-outcomes-an-ultrastructural-study-of-fragments
#13
Somayyeh Safari, Mohammad Ali Khalili, Zeinab Barekati, Iman Halvaei, Morteza Anvari, Stefania A Nottola
The aim was to study the ultrastructure of cytoplasmic fragments along with the effect of cosmetic micromanipulation (CM) on the morphology and development of vitrified-warmed embryos as well as assisted reproductive technology (ART) outcomes. A total of 96 frozen embryo transfer (FET) cycles were included in this prospective randomized study. They were divided into three groups of CM (n=32), sham (n=32) and control (n=32). In the CM group, the vitrified- warmed embryos were subjected to fragments and coarse granules removal (cosmetic micromanipulation) after laser assisted zona hatching (LAH); sham group subjected only to LAH and no intervention was taken for the control group...
May 17, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28511996/comparative-characteristics-of-spermatozoa-harvested-and-cryopreserved-in-culture-and-cryoprotectant-media-with-or-without-donor-serum-proteins
#14
Ghofraan A Ata'Allah, Noor Azmi Mat Adenan, Nuguelis Razali, Kannappan Palaniappan, Rosliza Saad, Siti Khadijah Idris, Krishnan Kanniah, Jaffar Ali
The objectives of this study is to evaluate the efficacy of protein-free media in the preparation, holding and crypreservation of spermatazoa for use in ART. Normozoospermic semen samples (N=71) were used to compare the effects of media on the survival and quality of spermatozoa when washed and cultured with different media with and without added proteins at 4°C, 15°C, 22°C and 37°C for 0, 4-7 and 24h. Survival and quality of spermatozoa were assessed after freeze-thaw with synthetic cryoprotectant with and without proteins...
May 13, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28511995/live-full-term-mouse-pups-from-oocytes-grown-and-matured-in-vitro-with-serum-substitutes
#15
Hideyuki H Motohashi, Ryoma Taniguchi, Junpei Sakamoto, Tadashi Sankai, Hidemi Kada
For in vitro growth and maturation of mouse oocytes (IVG-IVM), serum is added to media up to and including the stage of oocyte maturation; this subsequently supports oocytes through fertilization and early embryo development. However, problems may occur with sera, such as batch differences and issues of biosafety. The purpose of the present study was to determine the capacity for fertilization and pre- and post-implantation development of oocytes that underwent IVG-IVM with a serum substitute. Oocyte-granulosa cell complexes from preantral follicles were cultured in medium with either fetal bovine serum (FBS), Serum Substitute Supplement™ (SSS), or Knockout™ Serum Replacement (KSR) for 10days, and were then allowed to mature for 17 h...
May 13, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28479126/low-serum-concentration-in-bovine-embryo-culture-enhances-early-blastocyst-rates-on-day-6-with-quality-traits-in-the-expanded-blastocyst-stage-similar-to-bsa-cultured-embryos
#16
A Murillo, M Muñoz, D Martín-González, S Carrocera, A Martínez-Nistal, E Gómez
In bovine, single in vitro embryo culture in protein-free medium from Day-6 to Day-7 leads to expanded blastocyst (XB) with improved pregnancy and birth rates after cryopreservation. Under these conditions, early blastocysts (EB) progress to the XB stage at higher rates than morulae (M). However, embryo production with BSA in culture prior to Day-6 leads to low EB rates. We investigated whether a very low FCS concentration (0.1%) in culture from Day-1 to Day-6 would improve EB rates and, subsequently, increase XB rates on Day-7 after single culture in protein-free medium...
May 4, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28434778/live-birth-potential-of-good-morphology-and-vitrified-blastocysts-presenting-abnormal-cell-divisions
#17
Antonino Azzarello, Thomas Hoest, Anders Hay-Schmidt, Anne Lis Mikkelsen
This study included 238 good morphology blastocysts, which were transferred after vitrification-warming to 152 women by single blastocyst transfer in Holbæk Fertility Clinic, Denmark. Time-lapse recordings of transferred good morphology blastocysts were reassessed to recognize every abnormal cell division (ACD) from the 1st to the 4th cell cycle. ACDs were distinguished as failed cell divisions and multi-cell divisions. ACDs were recognized in 37.0% (no. 88/238) of good morphology blastocysts that were vitrified-warmed and transferred in our clinic...
April 20, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28434777/the-heat-shock-protein-60-promotes-progesterone-synthesis-in-mitochondria-of-jeg-3-cells
#18
Jessica Monreal-Flores, María Teresa Espinosa-García, Alejandro García-Regalado, Fabian Arechavaleta-Velasco, Federico Martínez
Progesterone synthesis in human placenta is essential to maintain pregnancy. The limiting step in placental progesterone synthesis is cholesterol transport from the cytoplasm to the inner mitochondrial membrane. Multiple proteins located in mitochondrial contact sites seem to play a key role in this process. Previously, our group identified the heat shock protein 60 (HSP60) as part of mitochondrial contact sites in human placenta, suggesting its participation in progesterone synthesis. Here, we examined the role of HSP60 in progesterone synthesis...
April 20, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28431992/genomic-answers-for-recurrent-spontaneous-abortion-in-saudi-arabia-an-array-comparative-genomic-hybridization-approach
#19
Sajjad Karim, Hasan Salleh Jamal, Abdullraheem Rouzi, Mohammed Salleh M Ardawi, Hans-Juergen Schulten, Zeenat Mirza, Nuha A Alansari, Maha M Al-Quaiti, Heba Abusamra, Muhammad Imran Naseer, Rola Turki, Adeel Gulzar Chaudhary, Mamdooh Gari, Adel Mohammed Abuzenadah, Mohammed Hussain Al-Qhatani
To study the genomics/genetic factors associated with recurrent spontaneous abortion (RSA), as ∼50% of RSA are unexplained. However, chromosome abnormalities have been reported to play major role in RSA. We performed whole genome array-CGH based genomic analysis of forty four Saudi RSA patients to identify potential molecular and chromosomal abnormalities. We identified a total of 845 alterations, usually not detected by classic cytogenetic methods, in different genomic regions using a cut off value of -0...
April 18, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28400095/anti-mullerian-hormone-as-a-parameter-for-endometrial-trauma-in-asherman-syndrome-a-retrospective-data-analysis
#20
Regina Promberger, Johannes Ott
Recently, the endometrium has been identified as a repository for Anti-Mullerian hormone (AMH), with endometrial masses associated with AMH serum levels. We aimed to compare AMH levels, as well as other parameters for ovarian reserve, in women with endometrial trauma due to Asherman syndrome (AS) and matched controls. In a retrospective study, nine women with hysteroscopically confirmed AS were compared to nine matched controls. Follicle-stimulating hormone, luteinizing hormone, and estradiol levels did not differ between women with and without AS, whereas significantly lower AMH levels were found in patients (median 0...
April 8, 2017: Reproductive Biology
journal
journal
40338
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"