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Reproductive Biology

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https://www.readbyqxmd.com/read/29764739/maternal-obesity-alters-the-expression-of-embryonic-regulatory-transcripts-in-the-preimplantation-ovine-conceptus
#1
Sarah R McCoski, Rebecca K Poole, McCauley T Vailes, Alan D Ealy
The influence of exposure to overfeeding-induced maternal obesity around the time of conception on early embryogenesis was examined in the day 14 ovine conceptus. The relative abundance of FGFR2 and DNMT1 was influenced by maternal obesity status and conceptus sex, and the abundance of PPARG and PTGS2 transcripts was greater in male conceptuses regardless of the obesity status of the ewe. These observations demonstrated that short-term exposure to maternal obesity impacts early conceptus transcript patterning...
May 12, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29729842/human-amniotic-epithelial-cells-inhibit-activation-and-pro-inflammatory-cytokines-production-of-naive-cd4-t-cells-from-women-with-unexplained-recurrent-spontaneous-abortion
#2
Hossein Motedayyen, Abbas Rezaei, Amir-Hassan Zarnani, Nader Tajik
Unexplained recurrent spontaneous abortion (URSA) has been assumed to be caused by a defect in maternal immunological tolerance to the fetus. Human amniotic epithelial cells (hAECs) have stem cell-like features and the ability to modulate the innate and adoptive immune responses. This study aimed to investigate whether hAECs have immunomodulatory effects on naive CD4+ T cells from URSA patients. hAECs were obtained from 15 healthy pregnant women and phenotypic profile of hAECs was determined by flow cytometry...
May 2, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29729841/cadmium-effects-on-sperm-morphology-and-semenogelin-with-relates-to-increased-ros-in-infertile-smokers-an-in-vitro-and-in-silico-approach
#3
Parameswari Ranganathan, Kamini A Rao, Jesu Jaya Sudan, Sridharan Balasundaram
Smoking releases cadmium (Cd), the metal toxicant which causes an imbalance in reactive oxygen species level in seminal plasma. This imbalance is envisaged to impair the sperm DNA morphology and thereby result in male infertility. In order to correlate this association, we performed in vitro and in silico studies and evaluated the influence of reactive oxygen species imbalance on sperm morphology impairments due to smoking. The study included 76 infertile smokers, 72 infertile non-smokers, 68 fertile smokers and 74 fertile non-smokers (control)...
May 2, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29705068/sperm-chromatin-alterations-in-fertile-and-subfertile-bulls
#4
Elisson Terêncio Souza, Cláudio Vieira Silva, Bruno Augusto Nassif Travençolo, Benner Geraldo Alves, Marcelo Emílio Beletti
Alterations in sperm chromatin have been related with subfertility in several mammals. In this study, chromatin alteration types (Base, Basal half, Central axis, Dispersed, and Whole) were assessed by toluidine blue (TB) staining, 6-diamidino-2-fenilindole (DAPI) and anti-protamine 1 antibody (anti-PR1) labeling in sperm samples of fertile and subfertile bulls. Semen samples were obtained from bulls kept in Artificial Insemination Center (fertile bulls) or from bulls subjected to scrotal insulation (subfertile bulls)...
April 25, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29602611/effects-of-melatonin-supplementation-prior-to-ovsynch-protocol-on-ovarian-activity-and-conception-rates-in-anestrous-murrah-buffalo-heifers-during-out-of-breeding-season
#5
Kavita, Jagat Bir Phogat, Anand Kumar Pandey, Ashok Kumar Balhara, Sarvpreet Singh Ghuman, Pankaj Gunwant
Buffalo heifers have tendency to show anestrus during summer season. Melatonin has been used for correcting summer dependent anestrous via inducing resumption of ovarian activity. Therefore, the investigation was conducted to compare efficacy of melatonin for induction of estrus and conception rate with Ovsynch protocol in summer anestrous Murrah buffalo heifers. Thirty, summer anestrous Murrah buffalo heifers were selected and divided into two groups- treatment (n = 20; 12 melatonin implants) and control (n = 10; no treatment)...
March 27, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29602610/successful-microdissection-testicular-sperm-extraction-for-men-with-non-obstructive-azoospermia
#6
REVIEW
Rauni Klami, Harri Mankonen, Antti Perheentupa
Non-obstructive azoospermia (NOA) is the most severe form of male infertility, defined by lack of spermatozoa in the ejaculate caused by impaired spermatogenesis. The chance of biological fatherhood of these men has been improved since the introduction of microdissection testicular sperm extraction (MD-TESE) combined with intracytoplasmic sperm injection. A thorough patient evaluation preoperatively is essential to recognize any underlying conditions, and to assist in patient counseling on the sperm recovery rate and pregnancy results...
March 27, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29567382/effect-of-short-and-medium-term-toxicity-of-doxorubicin-on-spermatogenesis-in-adult-wistar-rats
#7
Robson Campos Silva, Daniella Maria Coelho Britto, Wagner de Fátima Pereira, Gustavo Eustáquio Alvin Brito-Melo, Cristiane Tolentino Machado, Marcelo Mattos Pedreira
Doxorubicin (DXR) is a widely used chemotherapeutic anticancer agent that has potent activity against several solid and non-solid human malignant tumors, including childhood malignancies. However, DXR has serious toxic effects on tissues with rapid cell cycles, such as myeloid and lymphatic tissues, intestinal mucosa, testes and ovaries. In the present study, the short- and medium-term toxic effects of DXR on the reproductive system of male Wistar rats were evaluated using morphometric and stereological tools to quantify damage to the seminiferous epithelium...
March 20, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29526398/human-sperm-proteins-identified-by-2-dimensional-electrophoresis-and-mass-spectrometry-and-their-relevance-to-a-transcriptomic-analysis
#8
Karolina Nowicka-Bauer, Małgorzata Ozgo, Adam Lepczynski, Marzena Kamieniczna, Agnieszka Malcher, Wiesław Skrzypczak, Maciej Kurpisz
The aim of this study was to identify and analyse human sperm proteins from normozoospermic men using 2-dimensional electrophoresis (2-DE) and mass spectrometry (MS). We identified 73 different sperm proteins, including two less characterized human sperm proteins, Annexin A7 (ANXA7) and c14orf105. Bioinformatic analysis of detected sperm proteins revealed new carbohydrate and lipid metabolic pathways, which supply energy to motile sperm. A comparison of our data with available mRNA microarray data from the human testis allows for validation of identified sperm proteins and aids in the recognition of their physiological pathways...
March 8, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29454805/mouse-preantral-follicle-growth-in-3d-co-culture-system-using-human-menstrual-blood-mesenchymal-stem-cell
#9
Zahra Rajabi, Hossein Yazdekhasti, Seyed Mohammad Hossein Noori Mugahi, Mehdi Abbasi, Somaieh Kazemnejad, Abolfazl Shirazi, Masoumeh Majidi, Amir-Hassan Zarnani
Follicle culture provides a condition which can help investigators to evaluate various aspects of ovarian follicle growth and development and impact of different components and supplementations as well as presumably application of follicle culture approach in fertility preservation procedures. Mesenchymal Stem Cells (MSCs), particularly those isolated from menstrual blood has the potential to be used as a tool for improvement of fertility. In the current study, a 3D co-culture system with mice preantral follicles and human Menstrual Blood Mesenchymal Stem Cells (MenSCs) using either collagen or alginate beads was designed to investigate whether this system allows better preantral follicles growth and development...
March 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29454804/physiological-predictors-of-leptin-vary-during-menses-and-ovulation-in-healthy-women
#10
Kristyn E Sylvia, Tierney K Lorenz, Julia R Heiman, Gregory E Demas
Although research has shown interactions between the reproductive system and energy homeostasis, it is not clear how environmental or behavioral factors may factor into these associations. Here we aimed to determine how changes in reproductive state (i.e., phase of the menstrual cycle) and other behavioral and physiological factors may influence leptin levels in healthy women, as well as how sexual activity may play a role in leptin modulation. We collected serum and saliva from 32 healthy women and measured leptin, estradiol, and progesterone...
March 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29449095/relationships-between-bacteriospermia-dna-integrity-nuclear-protamine-alteration-sperm-quality-and-icsi-outcome
#11
Ali Zeyad, Mohammed Hamad, Houda Amor, Mohamad Eid Hammadeh
The Aim of this study was to evaluate the effects of bacteriospermia on human sperm parameters, nuclear protamines, DNA integrity and ICSI outcome in patients enrolled for ICSI treatment. 84 unselected couples consulting in infertility and obstetrics clinic and enrolled for ICSI treatment were included in this study. The semen specimens were screened bacteriologically; semen and sperm parameters were also evaluated according to WHO guidelines. DNA integrity, protamines concentration and protamine deficiency were estimated by TUNEL assay, AU-PAGE and Chromomycin (CMA3) respectively...
March 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29422377/icon-immunoconjugate-treatment-results-in-regression-of-red-lesions-in-a-non-human-primate-papio-anubis-model-of-endometriosis
#12
Demetra Hufnagel, Laura G Goetz, Zhiwei Hu, Atunga Nyachieo, Thomas D'Hooghe, Asgerally Fazleabas, Antoni Duleba, Graciela Krikun, Hugh S Taylor, Charles J Lockwood
Endometriosis is a common condition in reproductive-aged women characterized by ectopic endometrial lesions of varied appearance, including red, white, blue, black or powder burn coloration, which contribute to chronic pain and infertility. The immunoconjugate molecule (Icon) targets Tissue Factor, a transmembrane receptor for Factor VII/VIIa that is aberrantly expressed in the endothelium supporting ectopic endometrial tissue. Icon has been shown to cause regression of endometriosis in a murine model of disease but prior to this study had not been tested in non-human primates...
March 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29402603/monosodium-glutamate-impairs-the-contraction-of-uterine-visceral-smooth-muscle-ex-vivo-of-rat-through-augmentation-of-acetylcholine-and-nitric-oxide-signaling-pathways
#13
Mukti Mondal, Kaushik Sarkar, Partha Pratim Nath, Ashma Khatun, Sanghamitra Pal, Goutam Paul
The aim of the study was to examine the toxic effects of Monosodium glutamate (MSG), an extensively used food additive, on the contraction of uterine visceral smooth muscle (UVSM) in rat and to elucidate the probable neurocrine mechanism involved in it. MSG produced significant potentiation of the force and inhibition of frequency of uterus recorded ex vivo in chronic MSG exposure and in single dose acute experiments. MSG also produced significant potentiation of force of acetylcholine induced contraction and no alterations in atropine induced contraction of uterus...
March 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29396285/uterine-artery-flow-velocity-waveform-arterial-flow-indices-follicular-dynamics-and-sex-hormones-during-preovulatory-period-in-synchronized-ovulatory-cycle-of-bos-indicus-beef-cows
#14
Tossapol Moonmanee, Chainarong Navanukraw, Punnawut Yama, Jakree Jitjumnong
A greater understanding of the uterine artery's (UtA) biology is essential to the increase in female reproductive abilities. The UtA flow velocity waveform, blood flow volume (BFV), pulsatility and resistance indices (PI and RI), blood flow velocities, dynamics of the dominant follicle (DF), and estradiol (E2) and progesterone (P4) levels in an induced ovulatory cycle were evaluated in Thai native cattle. Twenty cows were induced with synchronized ovulation through a P4-releasing device, from Day -9 to Day -4, concurrent with the administration of two doses of a gonadotropin-releasing hormone on Day -9 and Day -1, and two doses of prostaglandin F2α on Day -4 and 8 h later...
March 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29396284/green-tea-extract-affects-porcine-ovarian-cell-apoptosis
#15
Shubhadeep Roychoudhury, Marek Halenar, Katarina Michalcova, Sangeeta Nath, Miroslava Kacaniova, Adriana Kolesarova
Green tea is a commonly used beverage and green tea extract is a common dietary herbal supplement manufactured into different over-the-counter products. The aim of this in vitro study was to examine the steroid hormone secretion (progesterone and 17-β estradiol), proliferation and apoptosis of porcine ovarian granulosa cells after addition of green tea extract. Granulosa cells were incubated with green tea extract at five doses (0.1, 1, 10, 100 and 200 μg/ml) and the release of hormones by granulosa cells was assessed by EIA after 24 h exposure...
March 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29371110/intramembranal-disulfide-cross-linking-elucidates-the-super-quaternary-structure-of-mammalian-catspers
#16
Christopher Bystroff
CatSper is a voltage-dependent calcium channel located in the plasma membrane of the sperm flagellum and is responsible for triggering hyperactive motility. A homology model for the transmembrane region was built in which the arrangement of the subunits around the pseudo-four-fold symmetry axis was deduced by the pairing of conserved transmembranal cysteines across mammals. Directly emergent of the predicted quaternary structure is an architecture in which tetramers polymerize through additional, highly conserved cysteines, creating one or more double-rows channels extending the length of the principal piece of the mammalian sperm tail...
March 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29361408/supplementing-in-vitro-embryo-production-media-by-nppc-and-sildenafil-affect-the-cytoplasmic-lipid-content-and-gene-expression-of-bovine-cumulus-oocyte-complexes-and-embryos
#17
Ramon Cesar Botigelli, Eduardo Montanari Razza, Elisa Mariano Pioltine, Patricia Kubo Fontes, Kátia Regina Lancellotti Schwarz, Cláudia Lima Verde Leal, Marcelo Fábio Gouveia Nogueira
In our study, we added natriuretic peptide type C (NPPC) and/or sildenafil during in vitro maturation (IVM) of bovine cumulus-oocyte complexes (COCs) followed by in vitro culture (IVC) of embryos with or without sildenafil. We evaluated the effects on the lipid content (LC) of oocytes and embryos and also verified the expression of 96 transcripts related to competence in matured COCs and 96 transcripts related to embryo quality in blastocysts. After IVM, LC was decreased in oocytes by NPPC while sildenafil did not affect LC in oocytes...
March 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29336947/characterization-of-goose-spms-molecular-characterization-and-expression-profiling-of-spms-in-the-goose-ovary
#18
Bo Kang, Qilin Xu, Ziyu Chen, Yongsheng Wu, Su Yang, Xicheng Yang, Zhao Zhang, Dongmei Jiang
Spermine synthase (SPMS), which converts spermidine into spermine, is essential for normal cell growth and development processes in humans and other mammals, but the molecular characterization and expression profiling of the SPMS gene remain undetermined in goose tissues and ovarian follicles. In this study, the SPMS cDNA sequence of the Sichuan white goose was cloned and analysed, and SPMS mRNA expression was profiled in various tissues and ovarian follicles. The results showed that the open reading frame of the SPMS cDNA sequence was 1092 bp in length, encoding 363 amino acids with a molecular weight of 41 kDa...
March 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29317175/premature-progesterone-rise-in-art-cycles
#19
REVIEW
Barbara Lawrenz, Laura Melado, Human Fatemi
Premature rise of progesterone during the late follicular phase in stimulated IVF cycles is a frequent event and its effect on the endometrial receptivity and on the ART (Assisted Reproductive Technique) - outcome has become a matter of intense debate and research. An emerging body of evidence demonstrates that premature progesterone rise does have a negative impact on the outcome of the ART-success. Until now, the exact cause of progesterone elevation is not fully clear, however lately published studies points to the fact, that premature progesterone elevation might be caused by enhanced FSH stimulation...
March 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29472137/synthesis-of-steroid-hormones-in-the-porcine-oviduct-during-early-pregnancy
#20
Marcin Martyniak, Anita Franczak, Genowefa Kotwica
Past studies of the oviducts have documented oviductal steroid production during the oestrous cycle in pigs. The present study examined whether the pig oviducts are the source of steroid hormones during early pregnancy. In the ampulla and isthmus, the expression of 3β-hydroxysteroid dehydrogenase (3βHSD) and aromatase cytochrome P450 (CYP19) mRNA by real-time PCR, cellular localization and quantities of the studied proteins by immunofluorescence and Western blot analysis, and concentration of steroid hormones in oviductal flushings by radioimmunoassay, were studied...
February 19, 2018: Reproductive Biology
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