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Reproductive Biology

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https://www.readbyqxmd.com/read/28434778/live-birth-potential-of-good-morphology-and-vitrified-blastocysts-presenting-abnormal-cell-divisions
#1
Antonino Azzarello, Thomas Hoest, Anders Hay-Schmidt, Anne Lis Mikkelsen
This study included 238 good morphology blastocysts, which were transferred after vitrification-warming to 152 women by single blastocyst transfer in Holbæk Fertility Clinic, Denmark. Time-lapse recordings of transferred good morphology blastocysts were reassessed to recognize every abnormal cell division (ACD) from the 1st to the 4th cell cycle. ACDs were distinguished as failed cell divisions and multi-cell divisions. ACDs were recognized in 37.0% (no. 88/238) of good morphology blastocysts that were vitrified-warmed and transferred in our clinic...
April 20, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28434777/the-heat-shock-protein-60-promotes-progesterone-synthesis-in-mitochondria-of-jeg-3-cells
#2
Jessica Monreal-Flores, María Teresa Espinosa-García, Alejandro García-Regalado, Fabian Arechavaleta-Velasco, Federico Martínez
Progesterone synthesis in human placenta is essential to maintain pregnancy. The limiting step in placental progesterone synthesis is cholesterol transport from the cytoplasm to the inner mitochondrial membrane. Multiple proteins located in mitochondrial contact sites seem to play a key role in this process. Previously, our group identified the heat shock protein 60 (HSP60) as part of mitochondrial contact sites in human placenta, suggesting its participation in progesterone synthesis. Here, we examined the role of HSP60 in progesterone synthesis...
April 20, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28431992/genomic-answers-for-recurrent-spontaneous-abortion-in-saudi-arabia-an-array-comparative-genomic-hybridization-approach
#3
Sajjad Karim, Hasan Salleh Jamal, Abdullraheem Rouzi, Mohammed Salleh M Ardawi, Hans-Juergen Schulten, Zeenat Mirza, Nuha A Alansari, Maha M Al-Quaiti, Heba Abusamra, Muhammad Imran Naseer, Rola Turki, Adeel Gulzar Chaudhary, Mamdooh Gari, Adel Mohammed Abuzenadah, Mohammed Hussain Al-Qhatani
To study the genomics/genetic factors associated with recurrent spontaneous abortion (RSA), as ∼50% of RSA are unexplained. However, chromosome abnormalities have been reported to play major role in RSA. We performed whole genome array-CGH based genomic analysis of forty four Saudi RSA patients to identify potential molecular and chromosomal abnormalities. We identified a total of 845 alterations, usually not detected by classic cytogenetic methods, in different genomic regions using a cut off value of -0...
April 18, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28400095/anti-mullerian-hormone-as-a-parameter-for-endometrial-trauma-in-asherman-syndrome-a-retrospective-data-analysis
#4
Regina Promberger, Johannes Ott
Recently, the endometrium has been identified as a repository for Anti-Mullerian hormone (AMH), with endometrial masses associated with AMH serum levels. We aimed to compare AMH levels, as well as other parameters for ovarian reserve, in women with endometrial trauma due to Asherman syndrome (AS) and matched controls. In a retrospective study, nine women with hysteroscopically confirmed AS were compared to nine matched controls. Follicle-stimulating hormone, luteinizing hormone, and estradiol levels did not differ between women with and without AS, whereas significantly lower AMH levels were found in patients (median 0...
April 8, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28363502/bovine-sperm-separation-by-swim-up-and-density-gradients-percoll-and-bovipure-effect-on-sperm-quality-function-and-gene-expression
#5
María Elena Arias, Katherine Andara, Evelyn Briones, Ricardo Felmer
This study assesses the effect of bovine sperm (obtained from three bulls) separation using density gradients (Percoll and BoviPure) and Swim-up on sperm function and gene expression. Sperm evaluations included the plasma membrane integrity (SYBR14/PI), acrosomal integrity (PNA-FITC/PI), oxidative stress (ROS; CH2FDDA), DNA fragmentation (TUNEL assay) and mitochondrial membrane potential (ΔYm; TMRM) using flow cytometry. Sperm motility was evaluated by computer-assisted sperm analysis (CASA) and gene expression using RT-qPCR...
March 28, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28336198/augmented-oxidative-stress-in-infertile-women-with-persistent-chlamydial-infection
#6
Jelena Tošić-Pajić, Dragana Šeklić, Jelena Radenković, Snežana Marković, Jelena Čukić, Dejan Baskić, Suzana Popović, Milos Todorovic, Predrag Sazdanović
There is established association between oxidative stress, infections of genital tract and fertility. Genital tract infections may provoke increased production of free radicals and generate oxidative stress that can be involved in pathophysiology of a number of reproductive diseases and complications during pregnancy. The aim of this study was to determine connection between oxidative stress and infertility associated with persistent chlamydial infection. Serum samples of infertile women with tubal factor infertility (TFI), women with multiple spontaneous abortions (MSA) and fertile women was screened for C...
March 20, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28262444/highly-potent-stem-cells-from-full-term-amniotic-fluid-a-realistic-perspective
#7
REVIEW
Adila A Hamid, Muhammad Khair Joharry, Hoo Mun-Fun, Siti Nurusaadah Hamzah, Zulida Rejali, Mohd Nazri Yazid, Thilakavathy Karuppiah, Dr Norshariza Nordin
Amniotic fluid (AF) is now known to harbor highly potent stem cells, making it an excellent source for cell therapy. However, most of the stem cells isolated are from AF of mid-term pregnancies in which the collection procedure involves an invasive technique termed amniocentesis. This has limited the access in getting the fluid as the technique imposes certain level of risks to the mother as well as to the fetus. Alternatively, getting AF from full-term pregnancies or during deliveries would be a better resolution...
March 2, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28215489/the-role-of-estrogen-receptor-subtypes-for-induction-of-delayed-effects-on-the-estrous-cycle-and-female-reproductive-organs-in-rats
#8
Miwa Takahashi, Ryohei Ichimura, Kaoru Inoue, Tomomi Morikawa, Kazunori Kuwata, Gen Watanabe, Midori Yoshida
It has been reported that neonatal exposure to estrogens at relatively low doses can induce early onset anovulation as a delayed effect in female rats. Dysfunction of kisspeptin neurons in the anteroventral periventricular nucleus (AVPV) was proposed to be a trigger for this effect. To determine the roles of estrogen receptor (ER) subtypes in the induction of delayed effects, we conducted a series of experiments using Donryu rats to examine whether neonatal injection of an ERα agonist (PPT), an ERβ agonist (DPN) or an ERα antagonist (ICI) could induce delayed effects...
February 16, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28215488/gonadotropin-releasing-hormone-agonist-gnrha-trigger-state-of-the-art
#9
REVIEW
C Dosouto, T Haahr, P Humaidan
GnRH agonist (GnRHa) trigger for final oocyte maturation in GnRH antagonist co-treated IVF/ICSI cycles significantly reduces the risk of ovarian hyperstimulation syndrome (OHSS). GnRHa trigger followed by modifications of the standard luteal phase support (modified luteal phase support) secures fresh transfer in the majority of patients with excellent reproductive outcomes. In freeze all cycles (segmented cycles) GnRHa trigger allows oocyte retrieval with a minimal risk of early onset OHSS and good reproductive outcomes in subsequent frozen thaw cycles...
February 16, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28215431/global-decrease-in-the-expression-of-signalling-pathways-genes-in-murine-uterus-during-preimplantation-pregnancy
#10
Katarzyna Buska, Anna Ewa Kedzierska, Anna Slawek, Anna Chelmonska-Soyta
Early preimplantation embryo-maternal communication is crucial for the establishment and development of pregnancy. Though the involvement of several candidate genes and proteins in this complex event has been described, the hierarchy of molecular networks governing this communication remains unknown. The primary objective of this study was to determine whether the presence of embryos in the uterine lumen stimulated or inhibited gene expression in the uterine tissue on day 3.5 post coitum. To answer this question, we investigated the gene expression of dedicated signal transduction pathways in the uterus of CD-1 mice during the preimplantation stage of pregnancy and compared this expression to mice with induced pseudopregnancy...
February 15, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28173995/gnrh-antagonist-and-letrozole-co-treatment-in-diminished-ovarian-reserve-patients-a-proof-of-concept-study
#11
Safak Olgan, Peter Humaidan
The current study aimed to investigate the effects of luteal gonadotropin-releasing hormone (GnRH) antagonist pretreatment on the outcomes of diminished ovarian reserve (DOR) patients who were treated using a FSH/letrozole/GnRH antagonist (FLA) protocol. Thus, patients who had luteal GnRH antagonist pretreatment (AFLA) prior to stimulation were compared to patients who had the FLA protocol, only. An electronic database was used to identify patients and stimulation characteristics. Women who had a total antral follicle count (AFC) of <7 were included in the analysis...
February 4, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28163019/interrelationships-between-ovarian-follicles-grown-in-culture-and-possible-mediators
#12
Alexander V Sirotkin, Iveta Florkovičová Koničková, Hans-Jorg Schaeffer, Jozef Laurincik, Abdel Halim Harrath
Dominance or cooperation between ovarian follicles can determine the number of ovulations and fecundity, but interrelationships between follicles in mono- and poly-ovulatory species and their mechanisms are poorly understood. The goals of this work were to determine the existence and compare the character of mutual influence of cultured ovarian follicles from a mono-ovulatory species (cow) with established follicular dominance with those from a poly-ovulatory species (pig), in which interrelationship between follicles remain unknown, and to examine the role of ovarian cell proliferation, the insulin-like growth factor I (IGF-I)- oxytocin (OT) system, and steroid hormones in mediating interrelationships among ovarian follicles...
February 2, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28132758/specific-ranges-of-anti-mullerian-hormone-and-antral-follicle-count-correlate-to-provide-a-prognostic-indicator-for-ivf-outcome
#13
K N Keane, V F Cruzat, S R Wagle, N G Chaudhary, P Newsholme, J L Yovich
Advanced female age correlates with reduced ovarian reserve (OR) and is the primary factor underlying the limitation of success rates in Assisted Reproductive Technology (ART). Currently, predicted ovarian response to gonadotrophin stimulation is determined using transvaginal ultrasound to measure antral follicle count (AFC), an indirect measure of OR. However, assessing the level of anti-Mullerian hormone (AMH) has more recently been shown to correlate with OR, and its application has been adopted widely. This retrospective study was designed to determine the relationship between novel ranges of AMH and AFC in patients undergoing ART...
January 26, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28082104/transcriptome-sequencing-analysis-of-porcine-granulosa-cells-treated-with-an-anti-inhibin-antibody
#14
Mingming Lei, Liuping Cai, Hui Li, Zhen Chen, Zhendan Shi
Inhibin can regulate granulosa cell proliferation and function via direct action on granulosa cells, or indirectly through stimulation of pituitary follicle-stimulating hormone secretion. Thus far, it has not been possible to unravel or formulate the chain of molecular events that lead to enhanced granulosa cell proliferation and function using conventional gene expression analysis. The aim of this study was to examine the biological effects of immuno-neutralization of inhibin bioactivity in porcine granulosa cells using transcriptome profiling by the RNA-seq technology...
January 9, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28077244/the-catsper-channel-modulates-boar-sperm-motility-during-capacitation
#15
Alejandro Vicente-Carrillo, Manuel Álvarez-Rodríguez, Heriberto Rodríguez-Martínez
The cation channel of sperm (CatSper) comprises four transmembrane subunits specifically expressed in human, equine, murine and ovine spermatozoa, apparently implicated in capacitation, hyperactivation and acrosome exocytosis. Western blotting and immunocytochemistry showed hereby that CatSper subunits are also present in boar spermatozoa, primarily over the sperm neck, tail and cytoplasmic droplets; albeit CatSper -1 presented in addition some distribution over the membrane of the acrosome and CatSper -2 and -4 over the membrane of the post-acrosome...
January 8, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28063736/brilliant-cresyl-blue-staining-does-not-present-cytotoxic-effects-on-human-luteinized-follicular-cells-according-to-gene-protein-expression-as-well-as-to-cytotoxicity-tests
#16
Diego Duarte Alcoba, Júlia Schneider, Letícia Arruda, Patrícia Borba Martiny, Edison Capp, Helena von Eye Corleta, Ilma Simoni Brum
In vitro oocyte maturation presents many advantages and its success is related to the selection of fully grown oocytes. In animal models, staining of cumulus-oocyte complexes (COCs) with Brilliant Cresyl Blue (BCB) is widely used for this selection. However, a lack of information about the safety of BCB makes its applicability in humans questionable. Because granulosa and cumulus cells have a close relationship with the oocyte and taking into account that follicular cells are also exposed to BCB when this pre-selection method is applied, we aimed to evaluate the effects of BCB on human follicular cells exposed to BCB...
January 4, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28041717/the-effect-of-cholesterol-loaded-cyclodextrins-on-post-thawing-quality-of-buffalo-semen-in-relation-to-sperm-dna-damage-and-ultrastructure
#17
M F Abouelezz, A E Montaser, M S Hussein, A M Eldesouky, M R Badr, A O Hegab, A Z Balboula, S M Zaabel
The cryopreservation of germ cells is a major tool for the propagation of animals with desired genetic traits. Although cryopreservation of spermatozoa in some animals is effective, its effectiveness is variable. For example, cryopreservation efficiency of buffalo bull spermatozoa remains very poor. In this study, we evaluated sperm DNA damage and ultrastructure in buffalo bull spermatozoa vitrified in the presence or absence of cholesterol-loaded cyclodextrins (CLC). Our results showed that cryopreserved buffalo spermatozoa had elevated levels of deteriorated plasma and mitochondrial membranes, which are the likely causes of DNA damage after vitrification...
December 29, 2016: Reproductive Biology
https://www.readbyqxmd.com/read/28040471/developmental-effects-of-imatinib-mesylate-on-follicle-assembly-and-early-activation-of-primordial-follicle-pool-in-postnatal-rat-ovary
#18
Babak Asadi-Azarbaijani, Regiane R Santos, Kirsi Jahnukainen, Saskia Braber, Majorie B M van Duursen, Jorma Toppari, Ola D Saugstad, Mirja Nurmio, Irma C Oskam
Imatinib mesylate is an anti-cancer agent that competitively inhibits several receptor tyrosine kinases (RTKs). RTKs play important roles in the regulation of primordial follicle formation, the recruitment of primordial follicles into the pool of growing follicles and maturation of the follicles. In the present study, we investigated the effects of the tyrosine kinase inhibitor imatinib on primordial follicle assembly and early folliculogenesis in postnatal rats. Female Sprague-Dawley rats were treated with either imatinib (150mg/kg) or placebo (water) on postnatal days 2-4...
December 28, 2016: Reproductive Biology
https://www.readbyqxmd.com/read/27964842/live-birth-rates-are-satisfactory-following-multiple-ivf-treatment-cycles-in-poor-prognosis-patients
#19
Kamarul B Mustafa, Kevin N Keane, Nikita L Walz, Katarina I Mitrovic, Peter M Hinchliffe, John L Yovich
This seven-year retrospective study analysed the live birth rate (LBR) for women undergoing IVF treatment with various antral follicle counts (AFC). The LBR decreased with lower AFC ratings, and in 290 treatment cycles for women in the poorest AFC category, ≤4 follicles (group E), the LBR was the lowest at 10.7%. The pregnancy loss rate (PLR) significantly increased with poorer AFC categories, from 21.8% in AFC group A (≥20 follicles), to 54.4% in AFC group E (p<0.0001). This trend was repeated with advancing age, from 21...
December 7, 2016: Reproductive Biology
https://www.readbyqxmd.com/read/27843090/superovulation-at-a-specific-stage-of-the-estrous-cycle-determines-the-reproductive-performance-in-mice
#20
Wilasinee Inyawilert, Yu-Jing Liao, Pin-Chi Tang
Inconsistent reproductive performance has been reported in superovulated mice. Hence, the aim of this study was to analyze the effect and possible mechanism of superovulation timing on mouse reproductive performance. The results showed that mice superovulated at the metestrous (23.08±6.08%) and diestrous stages (33.33±11.45%) presented significantly lower pregnancy rates compared with those superovulated at the estrous stage (66.67±9.20%). After superovulation at the proestrous and estrous stages, mucin 1 (MUC1) and let-7a/let-7b microRNA (miRNA) expression levels were significantly attenuated and enhanced on embryonic day 3...
December 2016: Reproductive Biology
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