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Reproductive Biology

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https://www.readbyqxmd.com/read/29325695/investigation-of-the-uterine-structural-changes-in-the-experimental-model-with-polycystic-ovary-syndrome-and-effects-of-vitamin-d-treatment-an-ultrastructural-and-immunohistochemical-study
#1
Yurdun Kuyucu, Latife Seyran Çelik, Özge Kendirlinan, Özgül Tap, Ufuk Özgü Mete
In this study, we aimed to investigate the structural changes seen in the endometrium in experimental PCOS rat model and the effects of vitamin D treatment on these changes at immunohistochemical and electron microscopic levels. 24 prepubertal female rats were divided into three groups. Two groups were injected with dehydroepiandrosterone and one of them was treated with 1,25(OH)2 D3 at the same time. The control group was injected with sesame oil. At the end of the 28th day, the blood samples were collected...
January 8, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29317175/premature-progesterone-rise-in-art-cycles
#2
REVIEW
B Lawrenz, L Melado, H M Fatemi
Premature rise of progesterone during the late follicular phase in stimulated IVF cycles is a frequent event and its effect on the endometrial receptivity and on the ART (Assisted Reproductive Technique) - outcome has become a matter of intense debate and research. An emerging body of evidence demonstrates that premature progesterone rise does have a negative impact on the outcome of the ART-success. Until now, the exact cause of progesterone elevation is not fully clear, however lately published studies points to the fact, that premature progesterone elevation might be caused by enhanced FSH stimulation...
January 6, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29279182/elective-frozen-thawed-embryo-transfer-fet-in-women-at-risk-for-ovarian-hyperstimulation-syndrome
#3
J Zech, A Brandao, M Zech, K Lugger, S Neururer, H Ulmer, E Ruttmann
Elective cryopreservation of cultured embryos has become a treatment option for women at risk for ovarian hyperstimulation syndrome (OHSS). The aim of our study was to investigate the outcome of elective cryopreservation and consecutive frozen-thawed embryo transfer (FET) in a large IVF clinic in Austria. A total of 6104 controlled ovarian hyperstimulation cycles (COH) were performed on 2998 patients including 200 patients (6.7%) who were undergoing elective cryopreservation and FET due to high risk of OHSS...
December 24, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29279181/embryo-culture-conditions-are-significantly-improved-during-uninterrupted-incubation-a-randomized-controlled-trial
#4
Y Alhelou, N A Mat Adenan, J Ali
A parallel group superiority prospective randomised controlled trial was devised to compare the culture characteristics of human pre-implantation stage embryos during uninterrupted culture in a time lapse incubator (TLI) versus the conventional model of interrupted culture in a standard incubator (SI) under low oxygen tension using a single step medium. 221 patients aged 35-and-under, 124 patients aged between 36 and 39 and 86 patients aged 40-and-over years were randomised and cultured either in a SI or in a TLI...
December 23, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29229446/clinical-utility-of-decorin-in-follicular-fluid-as-a-biomarker-of-oocyte-potential
#5
Yuki Sawada, Takeshi Sato, Chieko Saito, Fumiko Ozawa, Yasuhiko Ozaki, Mayumi Sugiura-Ogasawara
This study investigated the concentration of decorin (DCN) in mature follicular fluid and the existence in the granulosa cells. It also investigated whether DCN is useful as a biomarker for outcomes of assisted reproductive technology (ART). A retrospective cohort study was performed involving 130 oocytes of 88 patients treated with ART because of unexplained infertility. The concentration of DCN in the follicular fluid (F-DCN) was 39.26ng/ml (median value); it was higher than that in serum. F-DCN of the oocytes fertilized by intracytoplasmic sperm injection (ICSI) was significantly lower than that of oocytes that were not fertilized (33...
December 8, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29233485/body-fat-affects-mouse-reproduction-ovarian-hormone-release-and-response-to-follicular-stimulating-hormone
#6
Alexander V Sirotkin, Dušan Fabian, Janka Babeľová Kubandová, Radoslava Vlčková, Saleh Alwasel, Abdel Halim Harrath
We investigated the effects of body fat content on mouse fecundity, ovarian hormone release, and their response to follicle stimulation hormone (FSH). 4 types of females were produced: lean (group 1), normal (group 2), slightly fat (group 3), and significantly fat (group 4). The body weights, fat content, fertility rate, embryo number produced, retarded and degenerated embryo percentage, the release of progesterone (P4), testosterone (T), and insulin-like growth factor I (IGF-I) by isolated ovaries cultured with and without FSH (1...
December 7, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29221937/recurrent-miscarriage-is-associated-with-increased-ghrelin-mrna-expression-in-the-endometrium-a-case-control-study
#7
Aneta Zwierzchowska, Anna Iwan, Anna Hyc, Barbara Suchońska, Jacek Malejczyk, Ewa Barcz
Ghrelin has been found to be expressed in the human endometrium. Emerging evidence links ghrelin and its receptor with the reproductive system. Certain associations between ghrelin and angiogenesis have also been established. The aim of this small case-control study was to quantify and compare the expression of mRNA encoding ghrelin, ghrelin receptor (GHS-R), vascular endothelial growth factor A (VEGF A) and its receptors (VEGFR1-3) in the endometrium of women with recurrent miscarriage compared to parous controls...
December 5, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29196048/developmental-competence-and-apoptotic-gene-expression-patterns-of-mature-and-immature-human-oocytes-retrieved-from-controlled-ovarian-stimulation-cycles
#8
Farzaneh Fesahat, Mohammad Hasan Sheikhha, Seyed Mehdi Kalantar, Nasim Tabibnejad, Razieh Dehghani Firouzabadi, Hojjatollah Saeedi, Mohammad Ali Khalili
The purpose was to assess the developmental competence of the in vitro or in vivo matured human oocytes as well as the apoptotic genes expression of cumulus cells (CCs) regarding nuclear maturity status of associated oocytes retrieved from stimulated ICSI cycles. A total of 590 oocytes and the associated CCs were retrieved and divided into groups of test and control according to the nuclear maturity status in order to the developmental evaluation as well as expression patterns of apoptosis-related genes using real time PCR...
November 28, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29162325/scriptaid-improves-the-reprogramming-of-donor-cells-and-enhances-canine-porcine-interspecies-embryo-development
#9
Jin-Gu No, Tai-Young Hur, Minghui Zhao, Seunghoon Lee, Mi-Kyung Choi, Yoon-Seok Nam, Dong-Hyun Yeom, Gi-Sun Im, Dong-Hoon Kim
Histone methylation, histone acetylation, and DNA methylation are the important factors for somatic cell nuclear transfer (SCNT). Histone deacetylase inhibitors (HDACi) and DNA methyltransferase inhibitors (DNMTi) have been used to improve cloning efficiency. In particular, scriptaid, an HDACi, has been shown to improve SCNT efficiency. However, no studies have been performed on canines. Here, we evaluated the effects of scriptaid on histone modification in canine ear fibroblasts (cEFs) and cloned canine embryos derived from cEFs...
November 18, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29102762/immunoglobulins-from-sera-of-aps-patients-bind-htr-8-svneo-trophoblast-cell-line-and-reduce-additional-mediators-of-cell-invasion
#10
Milica Jovanović Krivokuća, Tamara Abu Rabi, Ivana Stefanoska, Svetlana Vrzić-Petronijević, Miloš Petronijević, Ljiljana Vićovac
Immunoglobulins from sera of patients with antiphospholipid syndrome (APS) decrease trophoblast cell invasion in vitro. This study aimed to extend understanding of cellular effects of immunoglobulins from APS (aPL+) in HTR-8/SVneo cells. aPL+ IgG induced change in effector molecules important for cell invasion was investigated further. After 1h of culture 21% cells bound aPL+ IgG, as opposed to 6% in control (aPL-). This was accompanied by increase in phospho-p38 at 30min. After 24h treatment aPL+IgG decreased protein levels of integrin subunits α1 (78% of control; p<0...
November 1, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29100724/expression-of-the-gm2-activator-protein-in-mouse-testis
#11
Yu-Teh Li, Su-Chen Li, I-Li Chen
The GM2-activator protein (GM2-AP), revealed by Li et al. in 1973 in human liver, was initially identified as a protein cofactor that stimulated β-hexosaminidase A to hydrolyze N-acetylgalactosamine from GM2 ganglioside. This cofactor was found to be missing in human variant AB Tay-Sachs disease. Over the years, the GM2-AP has also been shown to be involved in kidney vesicular transport, lipid presentation by CD1 molecule to T-cells, and interaction of human sperm with zona pellucida. Since the expression of the GM2-AP via mRNA detection in mouse tissues was found to be the highest in testis, we became interested in the localization of the GM2-AP at cellular level in mouse testis during spermatogenesis...
October 31, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29097083/knockdown-of-melatonin-receptor-1-and-induction-of-follicle-stimulating-hormone-on-the-regulation-of-mouse-granulosa-cell-function
#12
Hira Sajjad Talpur, Tesfaye Worku, Zia Ur Rehman, Rahim Dad, Dinesh Bhattarai, Iqra Bano, Farmanullah, Aixin Liang, Changjiu He, Liguo Yang
Melatonin receptor 1 (MT1) performs a critical role in the regulation of the animal reproductive system, particularly in follicular growth, and has a considerable effect on reproductive performance. However, the role that MT1 plays in regulating hormones associated with reproduction remains unclear. This study was designed to examine the physiological role of constitutive MT1 silencing and follicle stimulating hormone (FSH) treatment in reproduction, making use of mouse granulosa cells (mGCs) as a model. To understand the constitutive role of MT1 in ovarian physiology, the RNAi-Ready pSIREN-RETROQ-ZsGreen Vector mediated recombinant pshRNA was used to silence MT1 gene expression...
October 30, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29108863/alterations-in-sperm-dna-methylation-patterns-of-oligospermic-males
#13
Mohammed Laqqan, Sascha Tierling, Yaser Alkhaled, Christina LoPorto, Mohamad Eid Hammadeh
Aberrant in sperm DNA methylation patterns and histone modification play a role in male fecundity decline. This study was prepared to determine whether sperm DNA methylation at CpG dinucleotides is different in oligospermic males compared to proven fertile males and then to evaluate the correlation between the changes in sperm DNA methylation patterns and semen parameters of oligospermic males. A total of 165 males (64 proven fertile males "controls" and 101 oligospermic males "cases") were included in the study...
December 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29089199/microrna-27a-3p-affects-estradiol-and-androgen-imbalance-by-targeting-creb1-in-the-granulosa-cells-in-mouse-polycytic-ovary-syndrome-model
#14
Mingming Wang, Mei Liu, Jing Sun, Lina Jia, Shuoqian Ma, Jun Gao, Yunyun Xu, Hongwen Zhang, Suk Ying Tsang, Xiangdong Li
Polycystic ovary syndrome (PCOS) is a common endocrine abnormality in women characterized by a menstrual disturbance with chronic anovulation and hyperandrogenism, polycystic ovaries, and insulin resistance. MicroRNAs (miRNAs) are important fine-tune regulators involved in various physiological and pathological processes, but their actions are not fully understood. In this study, we observed the increased expression of miR-27a-3p in the ovaries of mice with PCOS and explored its functions in primary mouse granulosa cells (mGCs) and the mouse granulosa-like tumor cell line, KK-1, using several approaches...
December 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29055788/nitric-oxide-malondialdheyde-and-non-enzymatic-antioxidants-assessed-in-viable-spermatozoa-from-selected-infertile-men
#15
Elena Moretti, Giulia Collodel, Anna Ida Fiaschi, Lucia Micheli, Francesca Iacoponi, Daniela Cerretani
There are growing evidences that the semen of infertile male population shows higher reactive oxygen species (ROS) levels concomitant with lower antioxidant capacity compared to those detected in semen of fertile population. The plasma membrane of the sperm cell, which has high levels of polyunsaturated fatty acids, renders it particularly sensitive to ROS. The aim of this study was to compare the sperm parameters (concentration, motility, morphology and vitality) and the levels of malondialdehyde (MDA), as marker of lipid peroxidation (LPO), nitric oxide (NO), ascorbic acid (AA), total (GSHt) and oxidized glutathione (GSSG) in viable sperm in a group of 38 infertile patients and in a group of 55 control subjects with unknown reproductive potential...
October 18, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29031924/evaluation-of-cervical-mucus-and-reproductive-efficiency-of-seasonally-anovular-dairy-goats-after-short-term-progestagen-based-estrous-induction-protocols-with-different-gonadotropins
#16
Jeferson F Fonseca, Joanna M G Souza-Fabjan, Maria Emilia F Oliveira, Renata C Cruz, Luciana V Esteves, Maria Pia S L Matos de Paiva, Felipe Z Brandão, Antônio B Mancio
The use of three different gonadotropins was tested for estrous induction in dairy goats during the non-breeding season. All does received an injection of 30 μg of d-cloprostenol and intravaginal sponges containing 60mg of medroxyprogesterone acetate (MAP) for 6 d plus 20 IU of porcine FSH (pFSH), 200 IU of eCG or 250 IU of hCG 24h before sponge removal. In Experiment 1 (n=24), ovarian ultrasound parameters were recorded and cervical mucus was evaluated daily for 5 d after sponge removal or until ovulation...
October 11, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29030127/a-method-for-the-isolation-and-enrichment-of-purified-bovine-milk-exosomes
#17
Kanchan Vaswani, Yong Qin Koh, Fatema B Almughlliq, Hassendrini N Peiris, Murray D Mitchell
Exosomes are nanovesicles that play important roles in intercellular communication as they carry information to target cells. Isolation of high purity exosomes will aid in studying the exosomal cargo and quantity as well as how cell-specific messages are carried. We describe a new method incorporating size exclusion chromatography (SEC) to enrich milk-derived exosomes from extracellular vesicles (EVs). This involved the initial isolation of EVs from bovine milk via milk processing and ultracentrifugation; followed by a new method to enrich exosomes using SEC...
October 10, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29030126/the-pesticide-lindane-induces-dose-dependent-damage-to-granulosa-cells-in-an-in-vitro-culture
#18
Maria Grazia Palmerini, Gulmira Zhurabekova, Aru Balmagambetova, Stefania Annarita Nottola, Selenia Miglietta, Manuel Belli, Serena Bianchi, Sandra Cecconi, Valentina Di Nisio, Giuseppe Familiari, Guido Macchiarelli
Lindane, which is one of the most persistent organochlorine pesticide contaminating the Aral Sea region, is associated with numerous pathologies of the female reproductive system, including infertility, due to its gap junction blocker activity. By using an in vitro model of reproductive toxicity consisting of mouse parietal granulosa cells (GCs) exposed to increasing concentrations of Lindane ranging from 1 to 100μM (L1; L10; L100), we aimed to ascertain the Lindane toxicity by evaluating the ultrastructure and expression of the cell death protein p53...
October 10, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29030125/prolactin-gene-expression-in-the-pituitary-of-rats-subjected-to-vaginocervical-stimulation-requires-erk-1-2-signaling
#19
Patricia Reuquen, Emanuel Guajardo-Correa, María L Oróstica, Constanza Curotto, Alexis Parada-Bustamante, Hugo Cardenas, Pedro A Orihuela
Vaginocervical stimulation (VCS) induces twice-daily prolactin (PRL) surges resulting in pseudopregnancy in the rat. Furthermore, activation of the extracellular signal-regulated kinase-1/2 (Erk-1/2) is involved in the effect of estradiol (E2) on the Prl gene expression in pituitary cells. Herein, we investigated whether Erk-1/2 signaling is involved in the control of Prl expression in the pituitary of VCS rats and whether VCS regulates the effect of E2 on Erk-1/2 and Prl in the pituitary. Estrous rats were assigned as control or VCS groups and 0, 6, 12 or 24h later the levels and localization of phosphorylated Erk-1/2 (p-Erk-1/2) were analyzed in the pituitary...
October 10, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28988892/the-il-1%C3%AE-signalling-pathway-and-its-role-in-regulating-pro-inflammatory-and-pro-labour-mediators-in-human-primary-myometrial-cells
#20
Martha Lappas
Interleukin (IL)-1β plays a central role in the processes of human labour and delivery. The adaptor proteins involved in the IL-1β signalling pathway in human myometrium are not known. This study sought to determine the role of the adaptor proteins myeloid differentiation primary response 88 (MyD88), tumour necrosis factor receptor-associated factor 6 (TRAF6), IL-1 receptor-associated kinase 4 (IRAK4) and transforming growth factor beta-activated kinase 1 (TAK1) in IL-1β-induced formation of pro-inflammatory and pro-labour mediators in human myometrium...
October 6, 2017: Reproductive Biology
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