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Nephron. Experimental Nephrology

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https://www.readbyqxmd.com/read/25592271/inhalation-of-hydrogen-gas-is-beneficial-for-preventing-contrast-induced-acute-kidney-injury-in-rats
#1
Koichiro Homma, Tadashi Yoshida, Maho Yamashita, Kei Hayashida, Matsuhiko Hayashi, Shingo Hori
Background: The present study aimed at investigating the effect of a novel antioxidant, hydrogen (H2) gas, on the severity of contrast-induced acute kidney injury (CIAKI) in a rat model. Methods: CIAKI was induced in rats by intravenous injection of a contrast medium, Ioversol, in addition to reagents inhibiting prostaglandin and nitric oxide synthesis. During the injection of these reagents, the rats inhaled H2 gas or control gas. Results: One day after the injection, serum levels of urea nitrogen were significantly lower in H2 gas-inhaling CIAKI rats (17...
January 9, 2015: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25531215/stimulation-of-cyclooxygenase-2-expression-in-rat-peritoneal-mesothelial-cells
#2
Michael E Ullian, Louis M Luttrell, Mi-Hye Lee, Thomas A Morinelli
Objective: Since peritoneal dialysis causes peritoneal fibrosis, we examined how glucose (osmotic factor), mannitol (osmotic control), and angiotensin II (AngII) regulate proinflammatory cyclooxygenase 2 (COX-2) in primary rat peritoneal mesothelial cells. Materials and Methods: For this study, we used the following material (n = 4-8 cell lines): cells, passages 1-2; (125)I-AngII receptor surface binding (AT1R antagonist losartan, AT2R antagonist PD123319; both 10 µM); intracellular calcium probe calcium-5; COX-2 immunoblotting (β-actin normalized); real-time PCR of COX-2 gene PTGS2, and NF-κB inhibitor Ro-1069920 (5 µM)...
December 17, 2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25531933/polyuria-in-hantavirus-infection-reflects-disease-severity-and-is-associated-with-prolonged-hospital-stay-a-systematic-analysis-of-335-patients-from-southern-germany
#3
Joerg Latus, Daniel Kitterer, Juergen Dippon, Simon Müller, Ferruh Artunc, Stephan Segerer, M Dominik Alscher, Niko Braun
Background/Aims: Puumala virus (PUUV) infection leads to nephropathia epidemica (NE), especially in endemic areas in Central Europe. The clinical course of NE is characterized by acute kidney injury (AKI) with thrombocytopenia followed by polyuria to a different degree. The prevalence of polyuria and its associated risk factors have not been reported in a large cohort of NE patients. Methods: Clinical and laboratory data during the acute phase of the disease were obtained from the medical reports and files of 335 patients who received in-hospital treatment during acute hantavirus infection...
December 13, 2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25503637/beneficial-effects-of-amp-activated-protein-kinase-agonists-in-kidney-ischemia-reperfusion-autophagy-and-cellular-stress-markers
#4
Anne-Emilie Declèves, Kumar Sharma, Joseph Satriano
Background: Kidney ischemia-reperfusion is a form of acute kidney injury resulting in a cascade of cellular events prompting rapid cellular damage and suppression of kidney function. A cellular response to ischemic stress is the activation of AMP-activated protein kinase (AMPK), where AMPK induces a number of homeostatic and renoprotective mechanisms, including autophagy. However, whether autophagy is beneficial or detrimental in ischemia-reperfusion remains controversial. We investigated the effects of agonist induction of AMPK activity on autophagy and cell stress proteins in the model of kidney ischemia-reperfusion...
December 6, 2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25412793/wnt5a-is-necessary-for-normal-kidney-development-in-zebrafish-and-mice
#5
Liwei Huang, An Xiao, Soo Young Choi, Quane Kan, Weibin Zhou, Maria F Chacon-Heszele, Yun Kyoung Ryu, Sarah McKenna, Xiaofeng Zuo, Rejji Kuruvilla, Joshua H Lipschutz
BACKGROUND: Wnt5a is important for the development of various organs and postnatal cellular function. Little is known, however, about the role of Wnt5a in kidney development, although WNT5A mutations were identified in patients with Robinow syndrome, a genetic disease which includes developmental defects in kidneys. Our goal in this study was to determine the role of Wnt5a in kidney development. METHODS: Whole-mount in situ hybridization was used to establish the expression pattern of Wnt5a during kidney development...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25403022/protective-effects-of-relaxin-against-cisplatin-induced-nephrotoxicity-in-rats
#6
Takuya Yoshida, Hiromichi Kumagai, Tetsuya Kohsaka, Naoki Ikegaya
BACKGROUND: Cisplatin (CDDP)-induced acute kidney injury (AKI) involves pro-inflammatory responses, apoptosis of renal tubular epithelial cells and vascular damage. AKI increases the risk of chronic kidney disease. Relaxin (RLX) has anti-apoptotic and anti-fibrosis properties. The aim of this study was to investigate the effects of RLX on CDDP-induced nephrotoxicity. METHODS: We investigated the mitigating effects of RLX based on the etiopathology of AKI induced by CDDP, and also the anti-fibrotic effect of RLX on renal fibrosis after AKI...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25402740/multiple-mechanisms-in-renal-artery-stenosis-induced-renal-interstitial-fibrosis
#7
Rui Cui, Xiao Chen, Lei Peng, Jing Ma, Dan Zhu, Tong Li, Qiuju Wei, Bing Li
BACKGROUND/AIMS: Renal artery stenosis (RAS), which may lead to renal fibrosis, is a common cause of end-stage renal disease in elderly patients. However, the potential mechanisms leading to the development of renal fibrosis and atrophy have not been clarified. METHODS: A two-kidney, one-clip Goldblatt mouse model was established in the present study. Blood pressure, morphological and pathological alterations were examined on days 7, 14, and 28 after surgery. Peritubular capillary loss and pericyte changes after injury were evaluated...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25376933/increased-macrophage-activation-inhibited-by-tacrolimus-in-the-kidney-of-diabetic-rats
#8
Yonggui Wu, Yan Wang, Xiangming Qi, Pei Zhang, Chaoqun Zhang, Wei Zhang
BACKGROUND/AIMS: Accumulating evidence suggests that macrophage-induced inflammation may be the mechanism of development and progression of diabetic nephropathy. A previous study by our group has shown that tacrolimus, like cyclosporin A, has a renoprotective effect in diabetic rats. The present study aimed to elucidate the underlying molecular events. METHODS: Diabetic rats were induced by using streptozotocin. Diabetic rats were subjected to oral tacrolimus treatment at a dose of 0...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25376659/il-10-deficiency-increases-renal-ischemia-reperfusion-injury
#9
Xin Wan, Wen Juan Huang, Wen Chen, Hong-Guang Xie, Pan Wei, Xin Chen, Chang-Chun Cao
BACKGROUND: Renal ischemia-reperfusion (IR) injury is a frequent cause of acute kidney injury, which results in high morbidity and mortality. Inflammation is an important factor that is involved in kidney repair after renal IR injury. IL-10 is a potent anti-inflammatory cytokine that inhibits inflammatory pathways, but the role of IL-10 in repairing renal IR injury is not known. Here, we investigated the role of IL-10 in kidney repair after renal IR injury. METHODS: We used an IL-10(-/-) mouse model and examined the serologic and histomorphology of kidney after IR injury...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25376384/macrophage-depletion-ameliorates-glycerol-induced-acute-kidney-injury-in-mice
#10
Jin H Kim, Dong-Won Lee, Myeong H Jung, Hyun-Seop Cho, Dae-Hong Jeon, Se-Ho Chang, Dong Jun Park
BACKGROUND: This study was conducted to elucidate the role of renal macrophages in the development of acute kidney injury (AKI) in a glycerol (Gly)-induced rhabdomyolysis mouse model. METHODS: The experimental model of rhabdomyolysis requires injecting 50% Gly (10 ml/kg) intramuscularly into mice. Control mice were injected into the tail vein with the liposomal vehicle. Liposome-encapsulated clodronate (LEC)-only mice were injected with LEC. Gly-only mice were injected with Gly into a hind limb...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25376195/indoxyl-sulfate-induces-il-6-expression-in-vascular-endothelial-and-smooth-muscle-cells-through-oat3-mediated-uptake-and-activation-of-ahr-nf-%C3%AE%C2%BAb-pathway
#11
Yelixiati Adelibieke, Maimaiti Yisireyili, Hwee-Yeong Ng, Shinichi Saito, Fuyuhiko Nishijima, Toshimitsu Niwa
BACKGROUND/AIMS: Interleukin-6 (IL-6) is one of the inflammation biomarkers with highest predictive value for outcome in chronic kidney disease (CKD) patients. The present study aimed to determine the effects of indoxyl sulfate (IS) on IL-6 expression in vascular cells. METHODS: IS was administered to normo- and hypertensive rats. Human umbilical vein endothelial cells (HUVECs) and human aortic smooth muscle cells (HASMCs) were incubated with or without IS. RESULTS: Immunohistochemistry revealed that IS-administered rats showed increased expression of IL-6 in the aortic tissues...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25359386/role-of-reactive-oxygen-species-mediated-endoplasmic-reticulum-stress-in-contrast-induced-renal-tubular-cell-apoptosis
#12
Yifei Yang, Dingwei Yang, Dingping Yang, Ruhan Jia, Guohua Ding
BACKGROUND: Renal tubular cell apoptosis is a key mechanism of contrast-induced acute kidney injury. It has been reported that endoplasmic reticulum (ER) stress is the underlying mechanism of high osmolar contrast-induced renal tubular cell apoptosis. Whether ER stress is involved in low osmolar contrast-induced renal tubular cell injury remains unclear. In the present study, the roles of ER stress in iopromide-induced (a low osmolar contrast) renal tubular cell apoptosis and the effects of N-acetylcysteine (NAC) on ER stress were investigated...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25358915/benidipine-suppresses-in-situ-proliferation-of-leukocytes-and-slows-the-progression-of-renal-fibrosis-in-rat-kidneys-with-advanced-chronic-renal-failure
#13
Itsuro Kazama, Asuka Baba, Mitsunobu Matsubara, Yasuhiro Endo, Hiroaki Toyama, Yutaka Ejima
BACKGROUND/AIMS: Leukocytes, such as lymphocytes and macrophages, predominantly express delayed rectifier K(+) channels (Kv1.3) in their plasma membranes. In our previous study, the overexpression of these channels in leukocytes was strongly associated with their proliferation in kidneys and the progression of renal fibrosis in advanced-stage chronic renal failure (CRF). Since benidipine, a long-acting 1,4-dihydropyridine Ca(2+) channel blocker, is also highly potent as a Kv1.3 channel inhibitor, it could exert therapeutic efficacy in advanced CRF...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/25034792/recent-advances-in-animal-models-of-diabetic-nephropathy
#14
REVIEW
Boris Betz, Bryan R Conway
Diabetic nephropathy (DN) is the single most common cause of end-stage kidney disease. Therefore, it is imperative that novel therapies are developed. Progress has been hindered, however, by the lack of robust animal models. In the current review we describe recent advances in the field, including the impact of background strain, hypertension and transcriptomic profiling. While the C57BL/6J strain is relatively resistant to DN, the FVB strain appears more susceptible and Ove26 and db/db mice on this background may be useful in modelling types 1 and 2 DN, respectively...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/24993481/ischemia-reperfusion-of-unilateral-kidney-exaggerates-aging-induced-damage-to-the-heart-and-contralateral-kidney
#15
Junichiro Kato, Masaaki Nakayama, Wan-Jun Zhu, Takashi Yokoo, Sadayoshi Ito
AIMS: We aimed to determine the impact of aging on ischemic acute kidney injury, especially in terms of the pathological mechanisms of kidney and heart crosstalk. METHOD: The effects of 45 min of unilateral ischemic reperfusion (IR) of the renal artery on the contralateral kidney and heart were histologically assessed in 7- and 40-week-old SD rats after 7 days. RESULTS: Glomerular sclerosis, interstitial fibrosis and numbers of ED1 cells were significantly increased in the contralateral kidneys of the 40-, but not the 7-week-old rats...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/24923840/impact-of-chronic-kidney-disease-on-myocardial-blood-flow-regulation-in-dogs
#16
John G Kingma, Denys Simard, Pierre Voisine, Jacques R Rouleau
BACKGROUND/AIMS: Chronic kidney disease (CKD) increases cardiovascular risk possibly due to coronary microvessel dysfunction and impaired myocardial flow reserve. This study investigated the effects of CKD on the regulation and transmural distribution of myocardial blood flow along with oxygen demand during intravenous dobutamine-induced increases in cardiac work. METHODS: CKD was produced in dogs by a two-stage subtotal nephrectomy (kidney ablation-infarction model)...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/24923736/heat-shock-proteins-and-acute-ischaemic-kidney-injury
#17
REVIEW
Stephen O'Neill, Ewen M Harrison, James A Ross, Stephen J Wigmore, Jeremy Hughes
The incidence of acute kidney injury due to ischaemia-reperfusion injury (IRI) is rising but effective treatments and preventative approaches are currently lacking. IRI is also an inevitable consequence of kidney transplantation and significantly contributes to delayed graft function. Heat-shock proteins (Hsps) are highly conserved and ubiquitously expressed molecular chaperones that help maintain and restore normal cellular function in the kidney following IRI. Hsp70 is one of the most frequently studied Hsps because of potential cytoprotective properties and attractiveness as a therapeutic target...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/24863338/hydrogen-sulfide-ameliorates-high-glucose-toxicity-in-rat-peritoneal-mesothelial-cells-by-attenuating-oxidative-stress
#18
Ying Lu, Huaying Shen, Xiaosong Shi, Sheng Feng, Zhi Wang, Yongbing Shi
BACKGROUND/AIMS: Continuous exposure of the peritoneal membrane to high-glucose (HG) peritoneal dialysis fluids (PDFs) can produce peritoneal mesothelial cells (PMCs) injury. It has been demonstrated that hydrogen sulfide (H2S), the third endogenous gaseous mediator identified after nitric oxide and carbon monoxide, exhibits a potent protective effect on cell activity. We studied the toxic effects of HG PDFs and their reversal by H2S on cultures of rat PMCs. METHODS: Synchronized confluent rat PMCs were incubated with 2...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/24863135/endoplasmic-reticulum-stress-induces-epithelial-mesenchymal-transition-through-autophagy-via-activation-of-c-src-kinase
#19
Soo Young Moon, Hyo Sang Kim, Kyeong Woo Nho, Young Joo Jang, Sang Koo Lee
BACKGROUND: Endoplasmic reticulum (ER) stress has been implicated in inducing epithelial-mesenchymal transition (EMT). ER stress is also known to induce autophagy. However, it is unclear whether ER stress-induced autophagy contributes to EMT. We hypothesized that ER stress might induce EMT through autophagy via activation of c-Src kinase in tubular epithelial cells. METHOD: All experiments were performed using HK-2 cells. Protein expression was measured by Western blot analysis...
2014: Nephron. Experimental Nephrology
https://www.readbyqxmd.com/read/24855017/synergistic-effects-of-leflunomide-and-benazepril-in-streptozotocin-induced-diabetic-nephropathy
#20
Hua Jin, Shang Guo Piao, Ji Zhe Jin, Ying Shun Jin, Zhen Hua Cui, Hai Feng Jin, Hai Lan Zheng, Jin Ji Li, Yu Ji Jiang, Chul Woo Yang, Can Li
BACKGROUND: Leflunomide (LEF) and benazepril have renoprotective effects on diabetic nephropathy (DN) through their anti-inflammatory and anti-fibrotic activities. This study investigated whether combined treatment using LEF and benazepril affords superior protection compared with the respective monotherapies. METHODS: Diabetes was induced with streptozotocin (STZ, 65 mg/kg) by intraperitoneal injection in male Wistar rats. Two weeks after STZ injection, diabetic rats were treated daily for 12 weeks with LEF (10 mg/kg), benazepril (10 mg/kg), or a combination of both...
2014: Nephron. Experimental Nephrology
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