Bin Fu, Emma E Brock, Rebecca Andrews, Jonathan C Breiter, Ru Tian, Christina E Toomey, Joanne Lachica, Tammaryn Lashley, Mina Ryten, Nicholas W Wood, Michele Vendruscolo, Sonia Gandhi, Lucien E Weiss, Joseph S Beckwith, Steven F Lee
Super-resolution and single-molecule microscopies have been increasingly applied to complex biological systems. A major challenge of these approaches is that fluorescent puncta must be detected in the low signal, high noise, heterogeneous background environments of cells and tissue. We present RASP, Radiality Analysis of Single Puncta, a bioimaging-segmentation method that solves this problem. RASP removes false-positive puncta that other analysis methods detect and detects features over a broad range of spatial scales: from single proteins to complex cell phenotypes...
April 9, 2024: Journal of Physical Chemistry. B