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Reproductive Biology and Endocrinology: RB&E

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https://www.readbyqxmd.com/read/28923067/a-new-insight-into-male-fertility-preservation-for-patients-with-completely-immotile-spermatozoa
#1
Huanhua Chen, Guixue Feng, Bo Zhang, Hong Zhou, Caizhu Wang, Jinhui Shu, Xianyou Gan, Ruoyun Lin, Dongmei Huang, Yingqin Huang
BACKGROUND: Sperm cryopreservation is the most effective method to preserve male fertility but this is normally used for motile spermatozoa. Thus, only motile spermatozoa are used for cryopreservation in most reproductive medicine centers worldwide. The immotile spermatozoa from some problematic patients are usually discarded, resulting in a missed opportunity of sterility cryopreservation for future assisted reproductive treatments. Many studies have shown that successful fertilization can be obtained after selection of viable sperm from the completely immotile spermatozoa before ICSI...
September 18, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28874163/expression-of-factors-involved-in-apoptosis-and-cell-survival-is-correlated-with-enzymes-synthesizing-lysophosphatidic-acid-and-its-receptors-in-granulosa-cells-originating-from-different-types-of-bovine-ovarian-follicles
#2
Emilia Sinderewicz, Katarzyna Grycmacher, Dorota Boruszewska, Ilona Kowalczyk-Zięba, Joanna Staszkiewicz, Tomasz Ślężak, Izabela Woclawek-Potocka
BACKGROUND: Lysophosphatidic acid (LPA) regulates reproductive processes in the cow. Ovarian granulosa cells play a pivotal role in follicle growth and development. Nevertheless, the role of LPA in the local regulation of granulosa cell function in different follicle categories in the bovine ovary has not been investigated. METHODS: Ovarian follicles were divided into healthy, transitional and atretic categories. The expression levels of AX, PLA2, LPARs and factors involved in apoptosis and cell survival processes in granulosa cells in different types of follicles were measured by real-time PCR...
September 6, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28874155/the-regulation-of-ovary-and-conceptus-on-the-uterine-natural-killer-cells-during-early-pregnancy
#3
REVIEW
Han Gong, Yilu Chen, Jingjie Xu, Xingxing Xie, Dainan Yu, Bei Yang, Haibin Kuang
Uterine natural killer (uNK) cells are short-lived, terminally differentiated and the most abundant lymphocytes in the uterus which play a crucial role in the spiral arteriole modification and establishment of successful pregnancy. Dysregulation of uNK cells has been linked to gestational implications such as recurrent pregnancy loss, preeclampsia and fetal growth retardation. There is evidence showing that progesterone and estrogen can regulate the recruitment, proliferation, differentiation and function of uNK cells via direct action on intracellular nuclear receptors or through intermediary cells in the uterus during early pregnancy...
September 6, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28870217/controlled-ovulation-of-the-dominant-follicle-using-progestin-in-minimal-stimulation-in-poor-responders
#4
Qiuju Chen, Yun Wang, Lihua Sun, Shaozhen Zhang, Weiran Chai, Qingqing Hong, Hui Long, Li Wang, Qifeng Lyu, Yanping Kuang
BACKGROUND: The use of progestin (P) during ovarian stimulation is effective in blocking the luteinizing hormone (LH) surge in women with normal ovarian reserve, however, its effects have not been determined in poor responders. This study aimed to explore the follicular dynamics in P-primed minimal stimulation in poor responders. METHODS: A total of 204 infertile women with diminished ovarian reserve were allocated into the medroxyprogesterone acetate (MPA) group or the natural-cycle control group in an alternating order...
September 5, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28854933/does-daily-co-administration-of-letrozole-and-gonadotropins-during-ovarian-stimulation-improve-ivf-outcome
#5
Jigal Haas, Rawad Bassil, Jim Meriano, Nivin Samara, Eran Barzilay, Noa Gonen, Robert F Casper
BACKGROUND: For the last year we have been treating normal responders with gonadotropins and letrozole during the whole stimulation in order to improve response to FSH by increasing the intrafollicular androgen concentration, and to reduce circulating estrogen concentrations. The aim of this study was to compare the IVF outcome of normal responders treated with letrozole and gonadotropins during ovarian stimulation with patients treated with gonadotropins only. METHODS: A single centre retrospective cohort study of 174 patients (87 in each group)...
August 30, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28851440/plk1-is-essential-for-proper-chromosome-segregation-during-meiosis-i-meiosis-ii-transition-in-pig-oocytes
#6
Zixiao Zhang, Changchao Chen, Liying Ma, Qiuchen Yu, Shuai Li, Benazir Abbasi, Jiayi Yang, Rong Rui, Shiqiang Ju
BACKGROUND: Polo-like kinase 1 (Plk1), as a characteristic regulator in meiosis, organizes multiple biological events of cell division. Although Plk1 has been implicated in various functions in somatic cell mitotic processes, considerably less is known regarding its function during the transition from metaphase I (MI) to metaphase II (MII) stage in oocyte meiotic progression. METHODS: In this study, the possible role of Plk1 during the MI-to-MII stage transition in pig oocytes was addressed...
August 29, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28851386/the-decreased-expression-of-tipe2-protein-in-the-decidua-of-patients-with-missed-abortion-and-possible-significance
#7
Yingshuo Sun, Xiaoyan Wang, Yue Li, Han Sun, Lu Wan, Xishuang Wang, Lining Zhang, Zhenghui Fang, Zengtao Wei
BACKGROUND: Missed abortion is a common occurrence for otherwise healthy women. Immunological factor is one of the most important reasons. Tumor necrosis factor-α-induced protein-8 like-2 (TIPE2) is a novel negative immune regulator related to several human diseases. However, the expression level and clinical significance of TIPE2 in missed abortion remain unclear. METHODS: The expression of TIPE2 mRNA and protein in decidua and chorion from 36 missed abortion patients and 36 healthy controls was detected using quantitative real-time PCR, western blot and immunohistochemistry...
August 29, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28830470/determining-optimal-gestational-weight-gain-in-the-korean-population-a-retrospective-cohort-study
#8
Sae Kyung Choi, Guisera Lee, Yeon Hee Kim, In Yang Park, Hyun Sun Ko, Jong Chul Shin
BACKGROUND: The World Health Organization (WHO) international body mass index (BMI) cut-off points defining pre-pregnancy BMI categories in the Institute of Medicine (IOM) guidelines are not directly applicable to Asians. We aimed to define the optimal gestational weight gain (GWG) for the Korean population based on Asia-specific BMI categories. METHODS: Data from 2702 live singleton deliveries in three tertiary centers between 2010 and 2011 were analyzed retrospectively...
August 22, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28810916/the-catsper-channel-and-its-roles-in-male-fertility-a-systematic-review
#9
REVIEW
Xiang-Hong Sun, Ying-Ying Zhu, Lin Wang, Hong-Ling Liu, Yong Ling, Zong-Li Li, Li-Bo Sun
The Catsper channel is a sperm-specific, Ca(2+)-permeable, pH-dependent, and low voltage-dependent channel that is essential for the hyperactivity of sperm flagellum, chemotaxis towards the egg, capacitation and acrosome reaction. All of these physiological events require calcium entry into sperm cells. Remarkably, Catsper genes are exclusively expressed in the testis during spermatogenesis, and are sensitive to ion channel-induced pH change, such as NHEs, Ca(2+)ATPase, K(+) channel, Hv1 channel and HCO3(-) transporters...
August 15, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28810880/nlrp7-contributes-to-in-vitro-decidualization-of-endometrial-stromal-cells
#10
Jyun-Yuan Huang, Pei-Hsiu Yu, Yueh-Chun Li, Pao-Lin Kuo
BACKGROUND: Nucleotide-binding oligomerization domain (NACHT), leucine rich repeat (LRR) and pyrin domain (PYD) 7 containing protein, NLRP7, is a member of the NLR family which serves as innate immune sensors. Mutations and genetic variants of NLRP7 have been found in women with infertility associated conditions, such as recurrent hydatidiform mole, recurrent miscarriage, and preeclampsia. Decidualization of endometrial stromal cells is a hallmark of tissue remodeling to support embryo implantation and proper placental development...
August 15, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28806906/age-associated-mrna-expression-changes-in-bovine-endometrial-cells-in-vitro
#11
Nao Tanikawa, Ayaka Ohtsu, Ryouka Kawahara-Miki, Koji Kimura, Shuichi Matsuyama, Hisataka Iwata, Takehito Kuwayama, Koumei Shirasuna
BACKGROUND: Endometrial cells secrete various cytokines and the dysfunction of endometrial cells may directly lead to infertility. Interferon tau (IFNT) secreted by trophoblast cells, a well-known pregnancy recognition signal in ruminants, acts on the uterus to prepare for pregnancy. Aging causes cellular and organ dysfunction, and advanced maternal age is associated with reduced fertility. However, few studies have investigated age-dependent changes in the uterus. METHODS: Using next generation sequencing and real-time PCR, we examined mRNA expression in bovine endometrial cells in vitro obtained from young (mean 45...
August 14, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28806905/evaluation-of-the-cervix-tissue-homogeneity-by-ultrasound-elastography-in-infertile-women-for-the-prediction-of-embryo-transfer-ease-a-diagnostic-accuracy-study
#12
Antonio Stanziano, Anna Maria Caringella, Clementina Cantatore, Giuseppe Trojano, Ettore Caroppo, Giuseppe D'Amato
BACKGROUND: Ultrasound elastography is a non-invasive medical imaging technique able to quantitatively characterize the stiffness of a given tissue. It has been shown to predict the risk for cervical insufficiency and preterm delivery, and to allow differentiation of malignancy from normal tissue. The present study sought to evaluate whether cervical tissue dishomogeneity, as assessed by cervical ultrasound elastography, may predict the embryo transfer (ET) ease in infertile women undergoing IVF/ICSI...
August 14, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28800745/retraction-note-intracytoplasmic-morphologically-selected-sperm-injection-versus-conventional-intracytoplasmic-sperm-injection-a-randomized-controlled-trial
#13
Giovanni Battista La Sala, Alessia Nicoli, Eleonora Fornaciari, Angela Falbo, Ilaria Rondini, Daria Morini, Barbara Valli, Maria Teresa Villani, Stefano Palomba
No abstract text is available yet for this article.
August 11, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28789693/a-novel-method-to-demonstrate-that-pregnant-women-with-polycystic-ovary-syndrome-hyper-expose-their-fetus-to-androgens-as-a-possible-stepping-stone-for-the-developmental-theory-of-pcos-a-pilot-study
#14
Roy Homburg, Anil Gudi, Amit Shah, Alison M Layton
BACKGROUND: Polycystic ovary syndrome (PCOS), whose aetiology is unknown, is predominately a familial syndrome but confirmation of candidate genes has proved elusive. The developmental hypothesis for the origin of PCOS suggests that exposure of the fetus to excess androgens influences imprinting, leading to altered genetic expression in adult life. The aim of this pilot study was to examine whether the female fetus of a mother with PCOS is indeed exposed to excess androgens. METHODS: Using sebum production in the newborn as a surrogate for exposure to excess androgens during pregnancy thisprospective case control studyexamined whether neonatal sebum excretion is greater in female infants born to PCOS mothers compared to non-PCOS...
August 8, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28768516/anti-mullerian-hormone-is-linked-to-the-type-of-early-pregnancy-loss-in-idiopathic-recurrent-miscarriage-a-retrospective-cohort-study
#15
Sophie Pils, Natalia Stepien, Christine Kurz, Kazem Nouri, Regina Promberger, Johannes Ott
We correlated Anti-Mullerian hormone (AMH) levels and other parameters for ovarian reserve to the gestational age at the time of pregnancy loss in women with idiopathic recurrent miscarriage. In a retrospective study, 79 patients had suffered a total of 266 miscarriages. When comparing women with an "unembryonic" to those with an "embryonic" most recent miscarriage, there was no difference in median age (36.3 years, IQR 31.6-40.1 versus 34.2 years, IQR 29.9-38.0; p = 0.303) but in median AMH levels (0.7, IQR 0...
August 2, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28764714/testis-specific-histone-2b-is-associated-with-sperm-chromatin-dynamics-and-bull-fertility-a-pilot-study
#16
Naseer A Kutchy, Ana Velho, Erika S B Menezes, Marie Jacobsen, Giselle Thibaudeau, Robert W Wills, Arlindo Moura, Abdullah Kaya, Andy Perkins, Erdogan Memili
BACKGROUND: Bull fertility is the degree of sperm's ability to fertilize and activate the egg and support embryo development, and this is critical for herd reproductive performance. We used the bull as a unique model organism for the study of male fertility because cattle genetics and physiology is similar to those of other mammals including humans. Moreover, reliable fertility data along with well-established in vitro systems are available for bovine. The objective of this original study was to ascertain evolutionary diversification and expression dynamics of Testis Specific Histone 2B (TH2B) in sperm from Holstein bulls with different fertility scores...
August 1, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28750635/proteomic-analysis-of-human-follicular-fluid-associated-with-successful-in-vitro-fertilization
#17
Xiaofang Shen, Xin Liu, Peng Zhu, Yuhua Zhang, Jiahui Wang, Yanwei Wang, Wenting Wang, Juan Liu, Ning Li, Fujun Liu
BACKGROUND: Human follicular fluid (HFF) provides a key environment for follicle development and oocyte maturation, and contributes to oocyte quality and in vitro fertilization (IVF) outcome. METHODS: To better understand folliculogenesis in the ovary, a proteomic strategy based on dual reverse phase high performance liquid chromatography (RP-HPLC) coupled to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (LC-MALDI TOF/TOF MS) was used to investigate the protein profile of HFF from women undergoing successful IVF...
July 27, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28747195/oleic-acid-induces-down-regulation-of-the-granulosa-cell-identity-marker-foxl2-and-up-regulation-of-the-sertoli-cell-marker-sox9-in-bovine-granulosa-cells
#18
Vengala Rao Yenuganti, Jens Vanselow
During negative energy balance, the concentration of different fatty acids, especially of oleic acid (OA) increases in the follicular fluid of cattle. Previously, we showed that OA induced morphological, physiological and molecular changes in cultured bovine granulosa cells. In our present study we analyzed effects of OA on the expression of markers for granulosa and Sertoli cell identity, FOXL2 and SOX9, respectively, in addition to effects on the FOXL2 regulated genes ESR2, FST, PTGS2 and PPARG. The results showed that OA down-regulated FOXL2, ESR2, FST and PPARG but up-regulated PTGS2 and SOX9...
July 26, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28738839/evolution-of-metabolic-alterations-5%C3%A2-years-after-early-puberty-in-a-cohort-of-girls-predisposed-to-polycystic-ovary-syndrome
#19
Soren Harnois-Leblanc, Andréanne Trottier, Samuel Leblanc, Marie-Claude Battista, David H Geller, Jean-Patrice Baillargeon
BACKGROUND: We and others have observed that young girls predisposed to polycystic ovary syndrome (PCOS) display defective insulin sensitivity, beta-cell function and non-esterified fatty acids (NEFA) suppressibility during early pubertal years, compared to controls. Our objective is to assess whether these differences in glucose and NEFA metabolisms persist after 5 years in late/post-puberty. METHODS: We conducted a prospective cohort study between 2007 and 2015 with 4-6 years of follow-up in an academic institution research center...
July 24, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28732545/comment-on-oestrogen-induced-angiogenesis-and-implantation-contribute-to-the-development-of-parasitic-myomas-after-laparoscopic-morcellation
#20
LETTER
Ospan A Mynbaev, Antonio Malvasi, Sergei S Simakov, Andrea Tinelli
BACKGROUND: The cause of contamination and dissemination of leiomyoma tissue particles and cells in the peritoneal cavity during myomectomy is a challenging issue for both clinicians and researchers. Therefore, the article by Huang et al. recently published in your journal is the subject of this letter. MAIN BODY: We comment on the role of laparoscopic condition in xenograft implantation and also highlighted the shortcomings of this study. The surgical technique of intramural fibroid enucleation, cell spillage during morcellation and postsurgical hormonal impact on the development of parasitic myomas become evident, while the contribution of CO2 insufflation, the fibroid's nature, mutations and pseudocapsule impacts on angiogenesis are not clear...
July 20, 2017: Reproductive Biology and Endocrinology: RB&E
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