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Reproductive Biology and Endocrinology: RB&E

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https://www.readbyqxmd.com/read/29145898/making-gametes-from-alternate-sources-of-stem-cells-past-present-and-future
#1
REVIEW
Deepa Bhartiya, Sandhya Anand, Hiren Patel, Seema Parte
Infertile couples including cancer survivors stand to benefit from gametes differentiated from embryonic or induced pluripotent stem (ES/iPS) cells. It remains challenging to convert human ES/iPS cells into primordial germ-like cells (PGCLCs) en route to obtaining gametes. Considerable success was achieved in 2016 to obtain fertile offspring starting with mouse ES/iPS cells, however the specification of human ES/iPS cells into PGCLCs in vitro is still not achieved. Human ES cells will not yield patient-specific gametes unless and until hES cells are derived by somatic cell nuclear transfer (therapeutic cloning) whereas iPS cells retain the residual epigenetic memory of the somatic cells from which they are derived and also harbor genomic and mitochondrial DNA mutations...
November 16, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29100496/gene-expression-profiling-of-upregulated-mrnas-in-granulosa-cells-of-bovine-ovulatory-follicles-following-stimulation-with-hcg
#2
Jacques G Lussier, Mame N Diouf, Valérie Lévesque, Jean Sirois, Kalidou Ndiaye
BACKGROUND: Ovulation and luteinization of follicles are complex biological processes initiated by the preovulatory luteinizing hormone surge. The objective of this study was to identify genes that are differentially expressed in bovine granulosa cells (GC) of ovulatory follicles. METHODS: Granulosa cells were collected during the first follicular wave of the bovine estrous cycle from dominant follicles (DF) and from ovulatory follicles (OF) obtained 24 h following injection of human chorionic gonadotropin (hCG)...
November 3, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29096644/serum-periostin-levels-in-early-in-pregnancy-are-significantly-altered-in-women-with-miscarriage
#3
A Freis, J Schlegel, R J Kuon, A Doster, J Jauckus, T Strowitzki, A Germeyer
BACKGROUND: Miscarriage is a common complication in pregnancy and there is still a lack of biomarkers usable in asymptomatic patients before the event occurs. Periostin (PER), whose levels rise particularly during injury or inflammation, has been shown to play an important local role in implantation and early embryonic development. As PER has been described as a biomarker in various medical conditions we intended to evaluate if changes in PER serum levels may help to identify women at risk for spontaneous abortion in the first trimester...
November 2, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29065928/chromatin-status-and-transcription-factor-binding-to-gonadotropin-promoters-in-gonadotrope-cell-lines
#4
Huimin Xie, Hanne M Hoffmann, Anita K Iyer, Melissa J Brayman, Cindy Ngo, Mary Jean Sunshine, Pamela L Mellon
BACKGROUND: Proper expression of key reproductive hormones from gonadotrope cells of the pituitary is required for pubertal onset and reproduction. To further our understanding of the molecular events taking place during embryonic development, leading to expression of the glycoproteins luteinizing hormone (LH) and follicle-stimulating hormone (FSH), we characterized chromatin structure changes, imparted mainly by histone modifications, in model gonadotrope cell lines. METHODS: We evaluated chromatin status and gene expression profiles by chromatin immunoprecipitation assays, DNase sensitivity assay, and RNA sequencing in three developmentally staged gonadotrope cell lines, αT1-1 (progenitor, expressing Cga), αT3-1 (immature, expressing Cga and Gnrhr), and LβT2 (mature, expressing Cga, Gnrhr, Lhb, and Fshb), to assess changes in chromatin status and transcription factor access of gonadotrope-specific genes...
October 24, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29047395/cellular-and-molecular-characterization-of-gametogenic-progression-in-ex-vivo-cultured-prepuberal-mouse-testes
#5
J Isoler-Alcaraz, D Fernández-Pérez, E Larriba, J Del Mazo
BACKGROUND: Recently, an effective testis culture method using a gas-liquid interphase, capable of differentiate male germ cells from neonatal spermatogonia to spermatozoa has been developed. Nevertheless, this methodology needs deep analyses that allow future experimental approaches in basic, pathologic and/or reprotoxicologic studies. Because of this, we characterized at cellular and molecular levels the entire in vitro spermatogenic progression, in order to understand and evaluate the characteristics that define the spermatogenic process in ex vivo cultured testes compared to the in vivo development...
October 18, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29029620/tgf%C3%AE-superfamily-signaling-and-uterine-decidualization
#6
REVIEW
Nan Ni, Qinglei Li
Decidualization is an intricate biological process where extensive morphological, functional, and genetic changes take place in endometrial stromal cells to support the development of an implanting blastocyst. Deficiencies in decidualization are associated with pregnancy complications and reproductive diseases. Decidualization is coordinately regulated by steroid hormones, growth factors, and molecular and epigenetic mechanisms. Transforming growth factor β (TGFβ) superfamily signaling regulates multifaceted reproductive processes...
October 13, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29017513/association-between-tnf-il1b-il6-il10-and-ifng-polymorphisms-and-recurrent-miscarriage-a-case-control-study
#7
Jianting Ma, Xingguang Zhang, Gang He, Chunlin Yang
BACKGROUND: Approximately half of recurrent miscarriages have unexplained etiology. Recent evidences suggest that cytokines are important determinants in pregnancy maintenance and as such, cytokine gene polymorphisms, which can affect cytokine production and/or functionality, could play a role in the disorder. Thus, we aimed to investigate the association of selected cytokine gene polymorphisms with risk of recurrent miscarriage among Chinese. METHODS: TNF -238G > A, TNF -308G > A, IL1B -511 T > C, IL1B 3954C > T, IL6 -174G > C, IL6 -634C > G, IL10 -1082A > G and IFNG 874A > T polymorphisms were genotyped on 775 women with idiopathic recurrent miscarriage and 805 healthy parous control women...
October 10, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28992815/correction-to-expression-of-factors-involved-in-apoptosis-and-cell-survival-is-correlated-with-enzymes-synthesizing-lysophosphatidic-acid-and-its-receptors-in-granulosa-cells-originating-from-different-types-of-bovine-ovarian-follicles
#8
Emilia Sinderewicz, Katarzyna Grycmacher, Dorota Boruszewska, Ilona Kowalczyk-Zięba, Joanna Staszkiewicz, Tomasz Ślężak, Izabela Woclawek-Potocka
No abstract text is available yet for this article.
October 9, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28985764/identification-of-differential-genomic-dna-methylation-in-the-hypothalamus-of-pubertal-rat-using-reduced-representation-bisulfite-sequencing
#9
Lei Luo, Zhiqiu Yao, Jing Ye, Yuan Tian, Chen Yang, Xiaoxiao Gao, Min Song, Ya Liu, Yunhai Zhang, Yunsheng Li, Xiaorong Zhang, Fugui Fang
BACKGROUND: There are many variables affecting the onset of puberty in animals, including genetic, nutritional, and environmental factors. Recent studies suggest that epigenetic regulation, especially DNA methylation, plays a majorrole in the regulation of puberty. However, there have been no reports on DNA methylation of the pubertal genome. METHODS: We investigated DNA methylation in the female rat hypothalamus at prepuberty and puberty using reduced representation bisulfite sequencing technology...
October 6, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28974230/effect-of-day-3-embryo-morphometrics-and-morphokinetics-on-survival-and-implantation-after-slow-freezing-thawing-and-after-vitrification-warming-a-retrospective-cohort-study
#10
Elia Fernandez Gallardo, Carl Spiessens, Thomas D'Hooghe, Sophie Debrock
BACKGROUND: Morphometric and morphokinetic evaluation of in vitro cultured human embryos allows evaluation without time restriction and reduces intra- and inter-observer variability. Even though these technologies have been reported to improve the quality of cleavage stage embryo evaluation during fresh culture, possible advantages in the evaluation of cryopreserved embryos have been scarcely explored. This study aims to compare morphometric and morphokinetic parameters between slow frozen and vitrified embryos and to determine their relationship to embryo survival and implantation rate (IR) after thawing/warming...
October 3, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28974228/dichorionic-triplets-following-frozen-thawed-poor-stage-embryo-transfer-a-report-of-two-cases-and-a-review
#11
REVIEW
Atsushi Yanaihara, Shirei Ohgi, Kenichirou Motomura, Ryoma Taniguchi, Shota Hatakeyama, Takumi Yanaihara
BACKGROUND: We describe two cases of dichorionic triplet pregnancy after a frozen-thawed poor-stage embryo transfer. A 39-year-old and a 41-year-old woman underwent ART treatment. The first patient underwent intracytoplasmic sperm injection (ICSI) at 34 years of age, and two frozen-thawed poor-stage embryos were transferred at 39 years of age with assisted hatching, resulting in a trichorionic triamniotic triplet pregnancy. The second patient underwent ICSI, and two poor-grade blastocysts were transferred followed by assisted hatching, resulting in a dichorionic triamniotic triplet pregnancy...
October 3, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28969693/effects-of-melatonin-administration-on-embryo-implantation-and-offspring-growth-in-mice-under-different-schedules-of-photoperiodic-exposure
#12
Lu Zhang, Zhenzhen Zhang, Feng Wang, Xiuzhi Tian, Pengyun Ji, Guoshi Liu
BACKGROUND: Embryo implantation is crucial for animal reproduction. Unsuccessful embryo implantation leads to pregnancy failure, especially in human-assisted conception. Environmental factors have a profound impact on embryo implantation. Because people are being exposed to more light at night, the influence of long-term light exposure on embryo implantation should be explored. METHODS: The effects of long photoperiodic exposure and melatonin on embryo implantation and offspring growth were examined...
October 2, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28969648/ooplasmic-transfer-in-human-oocytes-efficacy-and-concerns-in-assisted-reproduction
#13
REVIEW
Sara Darbandi, Mahsa Darbandi, Hamid Reza Khorram Khorshid, Mohammad Reza Sadeghi, Ashok Agarwal, Pallav Sengupta, Safaa Al-Hasani, Mohammad Mehdi Akhondi
BACKGROUND: Ooplasmic transfer (OT) technique or cytoplasmic transfer is an emerging technique with relative success, having a significant status in assisted reproduction. This technique had effectively paved the way to about 30 healthy births worldwide. Though OT has long been invented, proper evaluation of the efficacy and risks associated with this critical technique has not been explored properly until today. This review thereby put emphasis upon the applications, efficacy and adverse effects of OT techniques in human...
October 2, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28938896/effect-of-local-endometrial-injury-in-proliferative-vs-luteal-phase-on-ivf-outcomes-in-unselected-subfertile-women-undergoing-in-vitro-fertilization
#14
Wenjie Liu, Reshef Tal, He Chao, Minghui Liu, Ying Liu
BACKGROUND: Mechanical endometrial injury prior to IVF has been suggested as a means to increase implantation rates by improving endometrial receptivity. However, the effects of endometrial injury in proliferative vs. luteal phase have not been studied before. This study aimed to explore whether endometrial injury in the proliferative phase of the preceding cycle before in vitro fertilization/embryo transfer (IVF-ET) improves the clinical outcomes in unselected subfertile women compared with injury in luteal phase...
September 22, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28938894/in-vitro-progesterone-production-by-luteinized-human-mural-granulosa-cells-is-modulated-by-activation-of-ampk-and-cause-of-infertility
#15
E C Bowdridge, M W Vernon, J A Flores, M J Clemmer
BACKGROUND: Mural granulosa cells from IVF patients were provided by the West Virginia University Center for Reproductive Medicine in Morgantown, WV. The effect of adenosine monophosphate activated protein kinase (AMPK) activation, primary cause of infertility, age, BMI, and pregnancy outcome on production of progesterone were examined separately. METHODS: Isolated mural sheets from IVF patients (n = 26) were centrifuged, supernatant discarded, and the pellet re-suspended in 500 μl of DMEM/F12...
September 22, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28923067/a-new-insight-into-male-fertility-preservation-for-patients-with-completely-immotile-spermatozoa
#16
Huanhua Chen, Guixue Feng, Bo Zhang, Hong Zhou, Caizhu Wang, Jinhui Shu, Xianyou Gan, Ruoyun Lin, Dongmei Huang, Yingqin Huang
BACKGROUND: Sperm cryopreservation is the most effective method to preserve male fertility but this is normally used for motile spermatozoa. Thus, only motile spermatozoa are used for cryopreservation in most reproductive medicine centers worldwide. The immotile spermatozoa from some problematic patients are usually discarded, resulting in a missed opportunity of sterility cryopreservation for future assisted reproductive treatments. Many studies have shown that successful fertilization can be obtained after selection of viable sperm from the completely immotile spermatozoa before ICSI...
September 18, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28874163/expression-of-factors-involved-in-apoptosis-and-cell-survival-is-correlated-with-enzymes-synthesizing-lysophosphatidic-acid-and-its-receptors-in-granulosa-cells-originating-from-different-types-of-bovine-ovarian-follicles
#17
Emilia Sinderewicz, Katarzyna Grycmacher, Dorota Boruszewska, Ilona Kowalczyk-Zięba, Joanna Staszkiewicz, Tomasz Ślężak, Izabela Woclawek-Potocka
BACKGROUND: Lysophosphatidic acid (LPA) regulates reproductive processes in the cow. Ovarian granulosa cells play a pivotal role in follicle growth and development. Nevertheless, the role of LPA in the local regulation of granulosa cell function in different follicle categories in the bovine ovary has not been investigated. METHODS: Ovarian follicles were divided into healthy, transitional and atretic categories. The expression levels of AX, PLA2, LPARs and factors involved in apoptosis and cell survival processes in granulosa cells in different types of follicles were measured by real-time PCR...
September 6, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28874155/the-regulation-of-ovary-and-conceptus-on-the-uterine-natural-killer-cells-during-early-pregnancy
#18
REVIEW
Han Gong, Yilu Chen, Jingjie Xu, Xingxing Xie, Dainan Yu, Bei Yang, Haibin Kuang
Uterine natural killer (uNK) cells are short-lived, terminally differentiated and the most abundant lymphocytes in the uterus which play a crucial role in the spiral arteriole modification and establishment of successful pregnancy. Dysregulation of uNK cells has been linked to gestational implications such as recurrent pregnancy loss, preeclampsia and fetal growth retardation. There is evidence showing that progesterone and estrogen can regulate the recruitment, proliferation, differentiation and function of uNK cells via direct action on intracellular nuclear receptors or through intermediary cells in the uterus during early pregnancy...
September 6, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28870217/controlled-ovulation-of-the-dominant-follicle-using-progestin-in-minimal-stimulation-in-poor-responders
#19
Qiuju Chen, Yun Wang, Lihua Sun, Shaozhen Zhang, Weiran Chai, Qingqing Hong, Hui Long, Li Wang, Qifeng Lyu, Yanping Kuang
BACKGROUND: The use of progestin (P) during ovarian stimulation is effective in blocking the luteinizing hormone (LH) surge in women with normal ovarian reserve, however, its effects have not been determined in poor responders. This study aimed to explore the follicular dynamics in P-primed minimal stimulation in poor responders. METHODS: A total of 204 infertile women with diminished ovarian reserve were allocated into the medroxyprogesterone acetate (MPA) group or the natural-cycle control group in an alternating order...
September 5, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28854933/does-daily-co-administration-of-letrozole-and-gonadotropins-during-ovarian-stimulation-improve-ivf-outcome
#20
Jigal Haas, Rawad Bassil, Jim Meriano, Nivin Samara, Eran Barzilay, Noa Gonen, Robert F Casper
BACKGROUND: For the last year we have been treating normal responders with gonadotropins and letrozole during the whole stimulation in order to improve response to FSH by increasing the intrafollicular androgen concentration, and to reduce circulating estrogen concentrations. The aim of this study was to compare the IVF outcome of normal responders treated with letrozole and gonadotropins during ovarian stimulation with patients treated with gonadotropins only. METHODS: A single centre retrospective cohort study of 174 patients (87 in each group)...
August 30, 2017: Reproductive Biology and Endocrinology: RB&E
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