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Journal of Nanobiotechnology

Doris Hinger, Susanna Gräfe, Fabrice Navarro, Bernhard Spingler, Devaraj Pandiarajan, Heinrich Walt, Anne-Claude Couffin, Caroline Maake
BACKGROUND: Photodynamic therapy (PDT) as promising alternative to conventional cancer treatments works by irradiation of a photosensitizer (PS) with light, which creates reactive oxygen species and singlet oxygen ((1)O2), that damage the tumor. However, a routine use is hindered by the PS's poor water solubility and extended cutaneous photosensitivity of patients after treatment. In our study we sought to overcome these limitations by encapsulation of the PS m-tetrahydroxyphenylchlorin (mTHPC) into a biocompatible nanoemulsion (Lipidots)...
October 3, 2016: Journal of Nanobiotechnology
Henrike Peuschel, Thomas Ruckelshausen, Silke Kiefer, Yuliya Silina, Annette Kraegeloh
BACKGROUND: Quantum dots (QDs) have great potential as fluorescent labels but cytotoxicity relating to extra- and intracellular degradation in biological systems has to be addressed prior to biomedical applications. In this study, human intestinal cells (Caco-2) grown on transwell membranes were used to study penetration depth, intracellular localization, translocation and cytotoxicity of CdSe/ZnS QDs with amino and carboxyl surface modifications. The focus of this study was to compare the penetration depth of QDs in differentiated vs undifferentiated cells using confocal microscopy and image processing...
September 26, 2016: Journal of Nanobiotechnology
Freya Joris, Daniel Valdepérez, Beatriz Pelaz, Stefaan J Soenen, Bella B Manshian, Wolfgang J Parak, Stefaan C De Smedt, Koen Raemdonck
BACKGROUND: While nanotechnology is advancing rapidly, nanosafety tends to lag behind since general mechanistic insights into cell-nanoparticle (NP) interactions remain rare. To tackle this issue, standardization of nanosafety assessment is imperative. In this regard, we believe that the cell type selection should not be overlooked since the applicability of cell lines could be questioned given their altered phenotype. Hence, we evaluated the impact of the cell type on in vitro nanosafety evaluations in a human and murine neuroblastoma cell line, neural progenitor cell line and in neural stem cells...
2016: Journal of Nanobiotechnology
Doris Hinger, Fabrice Navarro, Andres Käch, Jean-Sébastien Thomann, Frédérique Mittler, Anne-Claude Couffin, Caroline Maake
BACKGROUND: Photosensitizers are used in photodynamic therapy (PDT) to destruct tumor cells, however, their limited solubility and specificity hampers routine use, which may be overcome by encapsulation. Several promising novel nanoparticulate drug carriers including liposomes, polymeric nanoparticles, metallic nanoparticles and lipid nanocomposites have been developed. However, many of them contain components that would not meet safety standards of regulatory bodies and due to difficulties of the manufacturing processes, reproducibility and scale up procedures these drugs may eventually not reach the clinics...
2016: Journal of Nanobiotechnology
Irina B Alieva, Igor Kireev, Anastasia S Garanina, Natalia Alyabyeva, Antoine Ruyter, Olga S Strelkova, Oxana A Zhironkina, Varvara D Cherepaninets, Alexander G Majouga, Valery A Davydov, Valery N Khabashesku, Viatcheslav Agafonov, Rustem E Uzbekov
BACKGROUND: A new type of superparamagnetic nanoparticles with chemical formula Fe7C3@C (MNPs) showed higher value of magnetization compared to traditionally used iron oxide-based nanoparticles as was shown in our previous studies. The in vitro biocompatibility tests demonstrated that the MNPs display high efficiency of cellular uptake and do not affect cyto-physiological parameters of cultured cells. These MNPs display effective magnetocontrollability in homogeneous liquids but their behavior in cytoplasm of living cells under the effect of magnetic field was not carefully analyzed yet...
2016: Journal of Nanobiotechnology
Nahal R Lalefar, Andrzej Witkowski, Jens B Simonsen, Robert O Ryan
BACKGROUND: Wnt proteins modulate development, stem cell fate and cancer through interactions with cell surface receptors. Wnts are cysteine-rich, glycosylated, lipid modified, two domain proteins that are prone to aggregation. The culprit responsible for this behavior is a covalently bound palmitoleoyl moiety in the N-terminal domain. RESULTS: By combining murine Wnt3a with phospholipid and apolipoprotein A-I, ternary complexes termed nanodisks (ND) were generated...
2016: Journal of Nanobiotechnology
Chiranjib Chakraborty, Ashish Ranjan Sharma, Garima Sharma, Sang-Soo Lee
Presently, nanotechnology is a multi-trillion dollar business sector that covers a wide range of industries, such as medicine, electronics and chemistry. In the current era, the commercial transition of nanotechnology from research level to industrial level is stimulating the world's total economic growth. However, commercialization of nanoparticles might offer possible risks once they are liberated in the environment. In recent years, the use of zebrafish (Danio rerio) as an established animal model system for nanoparticle toxicity assay is growing exponentially...
2016: Journal of Nanobiotechnology
Lina Gällentoft, Lina M E Pettersson, Nils Danielsen, Jens Schouenborg, Christelle N Prinz, Cecilia Eriksson Linsmeier
BACKGROUND: A promising approach to improve the performance of neural implants consists of adding nanomaterials, such as nanowires, to the surface of the implant. Nanostructured interfaces could improve the integration and communication stability, partly through the reduction of the cell-to-electrode distance. However, the safety issues of implanted nanowires in the brain need to be evaluated and understood before nanowires can be used on the surface of implants for long periods of time...
2016: Journal of Nanobiotechnology
Katherine M Williams, Kuppan Gokulan, Carl E Cerniglia, Sangeeta Khare
BACKGROUND: The antimicrobial activity of silver nanoparticles (AgNP) has led to interest in their use in consumer products such as food contact materials, utensils, and storage containers. Incorporation of these materials into items intended for food processing and storage suggests that consumer use of these products could result in gastrointestinal exposure to AgNP, should the nanoparticles migrate from the product. The health impact of AgNP exposure is unknown, especially effects related to intestinal epithelial permeability and barrier function...
2016: Journal of Nanobiotechnology
Luan P Calegari, Roberto S Dias, Michelle D de Oliveira, Carine Ribeiro Pessoa, André S de Oliveira, Ana F C S Oliveira, Cynthia C da Silva, Flavio G Fonseca, Alice F Versiani, Sérgio O De Paula
BACKGROUND: In recent times, studies have demonstrated that carbon nanotubes are good candidates for use as vehicles for transfection of exogenous material into the cells. However, there are few studies evaluating the behavior of carbon nanotubes as DNA vectors and few of these studies have used multi-walled carbon nanotubes (MWCNTs) or carboxylated MWCNTs. Thus, this study aims to assess the MWCNTs' (carboxylated or not) efficiency in the increase in expression of the tetravalent vaccine candidate (TVC) plasmid vector for dengue virus in vitro using Vero cells, and in vivo, through the intramuscular route, to evaluate the immunological response profile...
2016: Journal of Nanobiotechnology
Lenka Štefančíková, Sandrine Lacombe, Daniela Salado, Erika Porcel, Eva Pagáčová, Olivier Tillement, François Lux, Daniel Depeš, Stanislav Kozubek, Martin Falk
BACKGROUND: Tumor targeting of radiotherapy represents a great challenge. The addition of multimodal nanoparticles, such as 3 nm gadolinium-based nanoparticles (GdBNs), has been proposed as a promising strategy to amplify the effects of radiation in tumors and improve diagnostics using the same agents. This singular property named theranostic is a unique advantage of GdBNs. It has been established that the amplification of radiation effects by GdBNs appears due to fast electronic processes...
2016: Journal of Nanobiotechnology
Marie Plissonneau, Jonathan Pansieri, Laurence Heinrich-Balard, Jean-François Morfin, Nathalie Stransky-Heilkron, Pascaline Rivory, Pierre Mowat, Mireille Dumoulin, Richard Cohen, Éric Allémann, Éva Tόth, Maria Joao Saraiva, Cédric Louis, Olivier Tillement, Vincent Forge, François Lux, Christel Marquette
BACKGROUND: Amyloidoses are characterized by the extracellular deposition of insoluble fibrillar proteinaceous aggregates highly organized into cross-β structure and referred to as amyloid fibrils. Nowadays, the diagnosis of these diseases remains tedious and involves multiple examinations while an early and accurate protein typing is crucial for the patients' treatment. Routinely used neuroimaging techniques such as magnetic resonance imaging (MRI) and positron emission tomography (PET) using Pittsburgh compound B, [(11)C]PIB, provide structural information and allow to assess the amyloid burden, respectively, but cannot discriminate between different amyloid deposits...
2016: Journal of Nanobiotechnology
Luisa H A Silva, Jaqueline R da Silva, Guilherme A Ferreira, Renata C Silva, Emilia C D Lima, Ricardo B Azevedo, Daniela M Oliveira
BACKGROUND: Nanoparticles' unique features have been highly explored in cellular therapies. However, nanoparticles can be cytotoxic. The cytotoxicity can be overcome by coating the nanoparticles with an appropriated surface modification. Nanoparticle coating influences biocompatibility between nanoparticles and cells and may affect some cell properties. Here, we evaluated the biocompatibility of gold and maghemite nanoparticles functionalized with 2,3-dimercaptosuccinic acid (DMSA), Au-DMSA and γ-Fe2O3-DMSA respectively, with human mesenchymal stem cells...
2016: Journal of Nanobiotechnology
Atul A Chaudhari, D'andrea Ashmore, Subrata Deb Nath, Kunal Kate, Vida Dennis, Shree R Singh, Don R Owen, Chris Palazzo, Robert D Arnold, Michael E Miller, Shreekumar R Pillai
BACKGROUND: Due to increasing antibiotic resistance, the use of silver coated single walled carbon nanotubes (SWCNTs-Ag) and antimicrobial peptides (APs) is becoming popular due to their antimicrobial properties against a wide range of pathogens. However, stability against various conditions and toxicity in human cells are some of the major drawbacks of APs and SWCNTs-Ag, respectively. Therefore, we hypothesized that APs-functionalized SWCNTs-Ag could act synergistically. Various covalent functionalization protocols described previously involve harsh treatment of carbon nanotubes for carboxylation (first step in covalent functionalization) and the non-covalently functionalized SWCNTs are not satisfactory...
2016: Journal of Nanobiotechnology
Savita Bisht, Martin Schlesinger, Alexander Rupp, Rolf Schubert, Jens Nolting, Jörg Wenzel, Stefan Holdenrieder, Peter Brossart, Gerd Bendas, Georg Feldmann
BACKGROUND: Pancreatic cancer is one of the most lethal of human malignancies known to date and shows relative insensitivity towards most of the clinically available therapy regimens. 3,5-bis(2-fluorobenzylidene)-4-piperidone (EF24), a novel synthetic curcumin analog, has shown promising in vitro therapeutic efficacy in various human cancer cells, but insufficient water solubility and systemic bioavailability limit its clinical application. Here, we describe nano-encapsulation of EF24 into pegylated liposomes (Lipo-EF24) and evaluation of these particles in preclinical in vitro and in vivo model systems of pancreatic cancer...
2016: Journal of Nanobiotechnology
Kata Kenesei, Kumarasamy Murali, Árpád Czéh, Jordi Piella, Victor Puntes, Emília Madarász
BACKGROUND: Precisely targeted nanoparticle delivery is critically important for therapeutic applications. However, our knowledge on how the distinct physical and chemical properties of nanoparticles determine tissue penetration through physiological barriers, accumulation in specific cells and tissues, and clearance from selected organs has remained rather limited. In the recent study, spectral imaging fluorescence microscopy was exploited for precise and rapid monitoring of tissue- and cell-type-specific distribution of fluorescent polystyrene nanoparticles with chemically distinct surface compositions...
2016: Journal of Nanobiotechnology
Sarit Cohen, Chen Gelber, Michal Natan, Ehud Banin, Enav Corem-Salkmon, Shlomo Margel
BACKGROUND: Isothiouronium salts are well known in their variety of antimicrobials activities. The use of polymeric biocides, polymers with antimicrobial activities, is expected to enhance the efficacy of some existing antimicrobial agents, thus minimizing the environmental problems accompanying conventional antimicrobials. METHODS: The current manuscript describes the synthesis and characterization of crosslinked polyisothiouronium methylstyrene (PITMS) nanoparticles (NPs) of narrow size distribution by dispersion co-polymerization of the monomer isothiouronium methylstyrene with the crosslinking monomer ethylene glycol dimetacrylate...
2016: Journal of Nanobiotechnology
Yiping He, Shakuntala Ingudam, Sue Reed, Andrew Gehring, Terence P Strobaugh, Peter Irwin
BACKGROUND: Magnesium oxide nanoparticles (MgO nanoparticles, with average size of 20 nm) have considerable potential as antimicrobial agents in food safety applications due to their structure, surface properties, and stability. The aim of this work was to investigate the antibacterial effects and mechanism of action of MgO nanoparticles against several important foodborne pathogens. RESULTS: Resazurin (a redox sensitive dye) microplate assay was used for measuring growth inhibition of bacteria treated with MgO nanoparticles...
2016: Journal of Nanobiotechnology
Monique Culturato Padilha Mendonça, Edilene Siqueira Soares, Marcelo Bispo de Jesus, Helder José Ceragioli, Silvia Pierre Irazusta, Ângela Giovana Batista, Marco Aurélio Ramirez Vinolo, Mário Roberto Maróstica Júnior, Maria Alice da Cruz-Höfling
BACKGROUND: We have previously demonstrated that reduced graphene oxide (rGO) administered intravenously in rats was detected inside the hippocampus after downregulation of the tight and adherens junction proteins of the blood-brain barrier. While down-regulators of junctional proteins could be useful tools for drug delivery through the paracellular pathway, concerns over toxicity must be investigated before clinical application. Herein, our purpose was to trace whether the rGO inside the hippocampus triggered toxic alterations in this brain region and in target organs (blood, liver and kidney) of rats at various time points (15 min, 1, 3 h and 7 days)...
2016: Journal of Nanobiotechnology
Marta Alonso-Nocelo, Raquel Abellan-Pose, Anxo Vidal, Miguel Abal, Noemi Csaba, Maria Jose Alonso, Rafael Lopez-Lopez, Maria de la Fuente
BACKGROUND: Metastases are the most common reason of cancer death in patients with solid tumors. Lymph nodes, once invaded by tumor cells, act as reservoirs before cancer cells spread to distant organs. To address the limited access of intravenously infused chemotherapeutics to the lymph nodes, we have developed PEGylated polyglutamic acid nanocapsules (PGA-PEG NCs), which have shown ability to reach and to accumulate in the lymphatic nodes and could therefore act as nanotransporters...
2016: Journal of Nanobiotechnology
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