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Annals of Clinical Microbiology and Antimicrobials

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https://www.readbyqxmd.com/read/28724383/molecular-characterization-and-genetic-relatedness-of-clinically-acinetobacter-baumanii-isolates-conferring-increased-resistance-to-the-first-and-second-generations-of-tetracyclines-in-iran
#1
Zahra Meshkat, Himen Salimizand, Yousef Amini, Mostafa Khakshoor, Davoud Mansouri, Hadi Farsiani, Kiarash Ghazvini, Adel Najafi
BACKGROUND: The increasing resistance of Acinetobacter baumannii to antibiotics has recently been regarded as a notable therapeutic difficulty. Evaluating resistance rates of some A. baumannii isolates to tetracyclines had an impact on understanding the antibiotic resistance dissemination. By comparing genetic characteristics and relatedness of A. baumannii isolates, we are able to determine the transition dynamics of outbreak isolates. METHODS: A total of 72 non-duplicate isolates of A...
July 19, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28701170/antimicrobial-susceptibility-among-gram-positive-and-gram-negative-organisms-collected-from-the-latin-american-region-between-2004-and-2015-as-part-of-the-tigecycline-evaluation-and-surveillance-trial
#2
Silvio Vega, Michael J Dowzicky
BACKGROUND: The in vitro activity of tigecycline and comparator agents was evaluated against Gram-positive and Gram-negative isolates collected in Latin American centers between 2004 and 2015 as part of the Tigecycline Evaluation and Surveillance Trial (T.E.S.T.) global surveillance study. METHODS: Minimum inhibitory concentrations (MICs) were determined using the broth microdilution methodology according to the Clinical and Laboratory Standards Institute (CLSI) guidelines...
July 12, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28693504/prevalence-of-multi-drug-resistant-enteropathogenic-and-enteroinvasive-escherichia-coli-isolated-from-children-with-and-without-diarrhea-in-northeast-indian-population
#3
Karuppasamy Chellapandi, Tapan Kumar Dutta, Indu Sharma, Surajit De Mandal, Nachimuthu Senthil Kumar, Lalsanglura Ralte
BACKGROUND: Diarrheagenic Escherichia coli are associated with infantile diarrhea in the developing countries. The present study was conducted to determine the occurrence and antimicrobial resistance pattern of enteropathogenic and enteroinvasive E. coli associated with diarrhoea among the paediatric patients. METHODS: A total of 262 stool samples were collected from children with and without diarrhea from Mizoram, Northeast India. E. coli were isolated and subjected to multiplex PCR to detect virulent genes of EPEC (eaeA and bfpA) and EIEC (ial)...
July 11, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28693493/comparison-of-in-house-and-commercial-real-time-pcr-based-carbapenemase-gene-detection-methods-in-enterobacteriaceae-and-non-fermenting-gram-negative-bacterial-isolates
#4
M Smiljanic, M Kaase, P Ahmad-Nejad, B Ghebremedhin
BACKGROUND: Carbapenemase-producing gram-negative bacteria are increasing globally and have been associated with outbreaks in hospital settings. Thus, the accurate detection of these bacteria in infections is mandatory for administering the adequate therapy and infection control measures. This study aimed to establish and evaluate a multiplex real-time PCR assay for the simultaneous detection of carbapenemase gene variants in gram-negative rods and to compare the performance with a commercial RT-PCR assay (Check-Direct CPE)...
July 10, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28676088/parasitological-and-biochemical-studies-on-cutaneous-leishmaniasis-in-shara-b-district-taiz-yemen
#5
Qhtan Asmaa, Salwa Al-Shamerii, Mohammed Al-Tag, Adam Al-Shamerii, Yiping Li, Bashir H Osman
BACKGROUND: The leishmaniasis is a group of diseases caused by intracellular haemoflagellate protozoan parasites of the genus Leishmania. Leishmaniasis has diverse clinical manifestations; cutaneous leishmaniasis (CL) is the most common form of leishmaniasis which is responsible for 60% of disability-adjusted life years. CL is endemic in Yemen. In Shara'b there is no reference study available to identify the prevalence of endemic diseases and no investigation has been conducted for diagnosing the diseases...
July 4, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28651533/profiling-of-antibiotic-resistance-of-bacterial-species-recovered-from-routine-clinical-isolates-in-ethiopia
#6
Robert-Jan Ten Hove, Melaku Tesfaye, Witold Frederik Ten Hove, Mesfin Nigussie
BACKGROUND: With the alarming rise in antibiotic resistance in African countries, the need for a surveillance system in the region has become pressing. The rapid expansion of data networks makes it possible to set up healthcare applications that can be both cost-efficient and effective. Large data sets are available for assessment of current antibiotic resistance among Ethiopian patients. Based on the data-presentation, a practical approach is proposed on how diagnostic laboratories can participate remedial action against antibiotic resistance in Ethiopia...
June 26, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28646898/prevalence-of-chlamydia-trachomatis-neisseria-gonorrhoeae-mycoplasma-genitalium-and-ureaplasma-urealyticum-infections-using-a-novel-isothermal-simultaneous-rna-amplification-testing-method-in-infertile-males
#7
Ling Qing, Qi-Xiang Song, Jian-Li Feng, Hai-Yan Li, Guiming Liu, Hai-Hong Jiang
BACKGROUND: The purpose of this study was to evaluate the prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium and Ureaplasma urealyticum infections in infertile men that consulted our outpatient departments using a novel simultaneous amplification testing (SAT) that is RNA-detection based. The possible impact of C. trachomatis, N. gonorrhoeae, M. genitalium and U. urealyticum infections on semen parameters was also noted in the present study. METHODS: A total of 2607 males that were diagnosed with infertility were included in this study...
June 24, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28629366/molecular-characterization-of-clinical-imp-producing-klebsiella-pneumoniae-isolates-from-a-chinese-tertiary-hospital
#8
Kaisheng Lai, Yanning Ma, Ling Guo, Jingna An, Liyan Ye, Jiyong Yang
BACKGROUND: IMP-producing Klebsiella pneumoniae (IMPKpn) exhibits sporadic prevalence in China. The mechanisms related to the spread of IMPKpn remain unclear. METHODS: Carbapenem non-susceptible K. pneumoniae isolates were collected from our hospital. The genetic relatedness, antimicrobial susceptibility, as well as sequence types (ST) were analyzed by pulsed-field gel electrophoresis (PFGE), VITEK 2 AST test Kit, and multilocus sequence typing (MLST), respectively...
June 19, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28583115/tuberculosis-arthritis-of-the-sternoclavicular-joint-after-uncomplicated-falciparum-malaria-a-case-report
#9
Boundia Djiba, Baidy Sy Kane, Mamadou Alpha Diallo, Khadim Diongue, Ngoné Diaba Diack, Hamidou Deme, Mouhamed Dieng, Maimouna Sow, Daouda Ndiaye, Abdoulaye Pouye
BACKGROUND: Malaria and tuberculosis are co-endemic in many developing countries. However their associations are rarely reported. Yet, it has been suggested that a pathological process may link the two diseases. CASE PRESENTATION: A 20-year-old female patient was admitted in the internal medicine service of Aristide Le Dantec Hospital for uncomplicated malaria. She was previously treated for autoimmune hemolytic anaemia using prednisone at 5 mg per day. Clinical examination showed swelling in front of the sternoclavicular joint...
June 5, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28558707/effect-of-the-2014-clinical-and-laboratory-standards-institute-urine-specific-breakpoints-on-cefazolin-susceptibility-rates-at-a-community-teaching-hospital
#10
Daniel B Chastain, Ijang Ngando, Christopher M Bland, Carlos Franco-Paredes, W Anthony Hawkins
BACKGROUND: Enterobacteriaceae, which include Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis, are identified as the infectious etiology in the majority of urinary tract infections (UTIs) in community hospitals across the United States. The minimum inhibitory concentration (MIC) is a useful tool when choosing an appropriate antibacterial agent. Recent changes to the 2014 Clinical and Laboratory Standards Institute (CLSI) guidelines included reporting a urine-specific cefazolin breakpoint for enterobacteriaceae (susceptible ≤16 mcg/mL)...
May 30, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28526094/patients-with-community-acquired-bacteremia-of-unknown-origin-clinical-characteristics-and-usefulness-of-microbiological-results-for-therapeutic-issues-a-single-center-cohort-study
#11
Johan Courjon, Elisa Demonchy, Nicolas Degand, Karine Risso, Raymond Ruimy, Pierre-Marie Roger
Bacteremia of unknown origin (BUO) are associated with increased mortality compared to those with identified sources. Microbiological data of those patients could help to characterize an appropriate empirical antibiotic treatment before bloodcultures results are available during sepsis of unknown origin. Based on the dashboard of our ward that prospectively records several parameters from each hospitalization, we report 101 community-acquired BUO selected among 1989 bacteremic patients from July 2005 to April 2016, BUO being defined by the absence of clinical and paraclinical infectious focus and no other microbiological samples retrieving the bacteria isolated from blood cultures...
May 19, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28526091/a-pre-therapeutic-coating-for-medical-devices-that-prevents-the-attachment-of-candida-albicans
#12
Diego Vargas-Blanco, Aung Lynn, Jonah Rosch, Rony Noreldin, Anthony Salerni, Christopher Lambert, Reeta P Rao
BACKGROUND: Hospital acquired fungal infections are defined as "never events"-medical errors that should never have happened. Systemic Candida albicans infections results in 30-50% mortality rates. Typically, adhesion to abiotic medical devices and implants initiates such infections. Efficient adhesion initiates formation of aggressive biofilms that are difficult to treat. Therefore, inhibitors of adhesion are important for drug development and likely to have a broad spectrum efficacy against many fungal pathogens...
May 19, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28511708/enzyme-mediated-formulation-of-stable-elliptical-silver-nanoparticles-tested-against-clinical-pathogens-and-mdr-bacteria-and-development-of-antimicrobial-surgical-thread
#13
Rupak Thapa, Chintan Bhagat, Pragya Shrestha, Suvash Awal, Pravin Dudhagara
BACKGROUND: Silver nanoparticles (AgNPs) are believed to be emerging tool against various infectious diseases including multi-drug resistant (MDR) bacteria. In the present study, in vitro synthesis of AgNPs was optimized using 1:50 ratio of macerozyme (25 μg/μl) and 1 mM AgNO3 incubated at 80 °C for 8 h. AgNPs were characterized by UV-Visible spectroscopy, dynamic light scattering (DLS), scanning electron microscopy, energy-dispersive X-ray spectroscopy, transmission electron microscopy (TEM) and X-ray diffraction (XRD)...
May 16, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28506229/determinants-of-felt-demand-for-dengue-vaccines-in-the-north-caribbean-region-of-colombia
#14
Yalil T Bracho-Churio, Ruth A Martínez-Vega, Alfonso J Rodriguez-Morales, Ronald G Díaz-Quijano, María L Luna-González, Fredi A Diaz-Quijano
BACKGROUND: The increasing burden associated with dengue in Latin America makes it essential to understand the community's interest in acquiring vaccines, as an input to plan its introduction in endemic regions. The objective of this study is to learn the felt demand for dengue vaccines by estimating the willingness to pay and its associated factors in endemic communities of the North Caribbean region of Colombia. METHODS: A population survey was administered from October to December 2015, including 1037 families in 11 municipalities in Colombia...
May 15, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28499446/genome-sequence-of-shigella-flexneri-strain-sp1-a-diarrheal-isolate-that-encodes-an-extended-spectrum-%C3%AE-lactamase-esbl
#15
Ping Shen, Jianzhong Fan, Lihua Guo, Jiahua Li, Ang Li, Jing Zhang, Chaoqun Ying, Jinru Ji, Hao Xu, Beiwen Zheng, Yonghong Xiao
BACKGROUND: Shigellosis is the most common cause of gastrointestinal infections in developing countries. In China, the species most frequently responsible for shigellosis is Shigella flexneri. S. flexneri remains largely unexplored from a genomic standpoint and is still described using a vocabulary based on biochemical and serological properties. Moreover, increasing numbers of ESBL-producing Shigella strains have been isolated from clinical samples. Despite this, only a few cases of ESBL-producing Shigella have been described in China...
May 12, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28490367/vitamin-d-deficiency-among-smear-positive-pulmonary-tuberculosis-patients-and-their-tuberculosis-negative-household-contacts-in-northwest-ethiopia-a-case-control-study
#16
Belay Tessema, Feleke Moges, Dereje Habte, Nebiyu Hiruy, Shewaye Yismaw, Kassahun Melkieneh, Yewulsew Kassie, Belaineh Girma, Muluken Melese, Pedro G Suarez
BACKGROUND: Vitamin D is a fat-soluble vitamin that increases the immunity against tuberculosis (TB), decreases the re-activation of latent TB and reduces the severity of active TB disease. Epidemiological studies on the prevalence of vitamin D deficiency, and its association with TB showed inconsistent results in different countries. This study was aimed to determine the prevalence of vitamin D deficiency and its association with TB in Northwest Ethiopia. METHODS: A case-control study was conducted among smear positive pulmonary tuberculosis patients and their household contacts without symptoms suggestive of TB...
May 11, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28486995/antimicrobial-resistance-and-virulence-characterization-of-staphylococcus-aureus-and-coagulase-negative-staphylococci-from-imported-beef-meat
#17
Kamelia Osman, Avelino Alvarez-Ordóñez, Lorena Ruiz, Jihan Badr, Fatma ElHofy, Khalid S Al-Maary, Ihab M I Moussa, Ashgan M Hessain, Ahmed Orabi, Alaa Saad, Mohamed Elhadidy
BACKGROUND: The objectives of this study were to characterize the diversity and magnitude of antimicrobial resistance among Staphylococcus species recovered from imported beef meat sold in the Egyptian market and the potential mechanisms underlying the antimicrobial resistance phenotypes including harboring of resistance genes (mecA, cfr, gyrA, gyrB, and grlA) and biofilm formation. RESULTS: The resistance gene mecA was detected in 50% of methicillin-resistant non-Staphylococcus aureus isolates (4/8)...
May 10, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28486994/the-emergence-of-a-novel-sequence-type-of-mdr-acinetobacter-baumannii-from-the-intensive-care-unit-of-an-egyptian-tertiary-care-hospital
#18
Doaa Mohammad Ghaith, Mai Mahmoud Zafer, Mohamed Hamed Al-Agamy, Essam J Alyamani, Rayan Y Booq, Omar Almoazzamy
BACKGROUND AND AIM OF WORK: Acinetobacter baumannii is known for nosocomial outbreaks worldwide. In this study, we aimed to investigate the antibiotic susceptibility patterns and the clonal relationship of A. baumannii isolates from the intensive care unit (ICU) of an Egyptian hospital. METHODS: In the present study, 50 clinical isolates of multidrug resistant (MDR)-A. baumannii were obtained from patients admitted into the ICU from June to December 2015. All isolates were analyzed for antimicrobial susceptibilities...
May 10, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28482925/detection-of-a-ctx-m-group-2-beta-lactamase-gene-in-a-klebsiella-pneumoniae-isolate-from-a-tertiary-care-hospital-trinidad-and-tobago
#19
Paul Cheddie, Francis Dziva, Patrick Eberechi Akpaka
BACKGROUND: Identification of the prevalence and spread of ESBL-mediated antibiotic resistance is essential especially in the hospital setting. It is for this reason, more and more studies are highlighting the importance of complementing phenotypic ESBL-detection techniques with molecular techniques in order to understand the basis and extent of this form of resistance among clinically evolved bacterial populations, especially those belonging to the Enterobacteriaceae family. However, in Trinidad and Tobago and other Caribbean countries, very little is known regarding ESBL detection rates and/or the prevalence of genes conferring ESBL resistance...
May 8, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28420384/molecular-characteristic-of-mcr-1-producing-escherichia-coli-in-a-chinese-university-hospital
#20
Qing-Wen He, Xiao-Hong Xu, Fang-Jun Lan, Zhi-Chang Zhao, Zhi-Yun Wu, Ying-Ping Cao, Bin Li
BACKGROUND: Colistin has been considered as a last-line treatment option in severe infections caused by multidrug-resistant (MDR) gram-negative pathogens. However, the emergence of the mobile colistin resistance gene (mcr-1) has challenged this viewpoint. The aim of this study is to explore the prevalence of mcr-1 in Escherichia coli (E. coli) in a Chinese teaching hospital, and investigate their molecular characteristics. METHODS: A total of 700 E. coli isolates were used to screen mcr-1 by PCR and sequencing in a Chinese university hospital from August 2014 to August 2015...
April 19, 2017: Annals of Clinical Microbiology and Antimicrobials
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