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Annals of Clinical Microbiology and Antimicrobials

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https://www.readbyqxmd.com/read/29776363/rapid-detection-of-carbapenemase-activity-of-enterobacteriaceae-isolated-from-positive-blood-cultures-by-maldi-tof-ms
#1
Jiajia Yu, Jingxian Liu, Yuanrui Li, Jing Yu, Weinan Zhu, Ying Liu, Lisong Shen
BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proved to be a useful tool for identification of pathogens directly isolated from blood cultures in clinical microbiology laboratories. β-Lactam antibiotics are commonly used for treatment of bloodstream infections caused by Enterobacteriaceae strains, and carbapenem is the superlative class of β-lactam antibiotics. Since the carbapenem resistance rate of Enterobacteriaceae strains raised year by year, efficient detection of carbapenemase activity and timely delivery of carbapenem susceptibility reports of Enterobacteriaceae strains isolated from blood cultures is important for clinicians...
May 18, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29747632/mycotic-aneurysm-caused-by-edwardsiella-tarda-successfully-treated-with-stenting-and-suppressive-antibiotic-therapy-a-case-report-and-systematic-review
#2
Kei Furui Ebisawa, Sho Nishimura, Shungo Yamamoto, Goh Ohji, Kentaro Iwata
BACKGROUND: Mycotic aneurysm is an uncommon disease which could be fatal without appropriate treatment. Although standard therapy for mycotic aneurysms consists of resection of the infected aorta and in situ graft replacement, some treat with endovascular stent-grafting because patients may not tolerate graft replacement due to underlying diseases. There are 6 more reported cases of mycotic aneurysm caused by Edwardsiella tarda. With the exception of our case, all underwent resection and debridement of the infected aorta or vascular prosthesis...
May 10, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29728111/emergence-of-multidrug-resistant-providencia-rettgeri-isolates-co-producing-ndm-1-carbapenemase-and-per-1-extended-spectrum-%C3%AE-lactamase-causing-a-first-outbreak-in-korea
#3
Saeam Shin, Seok Hoon Jeong, Hyukmin Lee, Jun Sung Hong, Min-Jeong Park, Wonkeun Song
BACKGROUND: Nosocomial outbreak due to carbapenem-resistant Enterobacteriaceae has become serious challenge to patient treatment and infection control. We describe an outbreak due to a multidrug-resistant Providencia rettgeri from January 2016 to January 2017 at a University Hospital in Seoul, Korea. METHODS: A total of eight non-duplicate P. rettgeri isolates were discovered from urine samples from eight patients having a urinary catheter and admitted in a surgical intensive care unit...
May 5, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29728115/nasal-colonization-with-methicillin-resistant-staphylococcus-aureus-among-elderly-living-in-nursing-homes-in-brazil-risk-factors-and-molecular-epidemiology
#4
Monica da Silveira, Maria de Lourdes Ribeiro de Souza da Cunha, Camila Sena Martins de Souza, Adriana Aparecida Feltrin Correa, Carlos Magno Castelo Branco Fortaleza
BACKGROUND: Methicillin-resistant Staphylococcus aureus poses a threat to elderly living in nursing homes. Studies focusing on the epidemiology of colonization may help in the design of infection control strategies. OBJECTIVE: To identify factors associated with MRSA colonization and the dissemination of clones among nursing home residents. METHODS: Nasal swabs were collected from 300 persons from nine nursing homes in the city of Bauru, Brazil...
May 4, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29728100/raoultella-planticola-bacteremia-induced-fatal-septic-shock-following-burn-injury
#5
Tetsuya Yumoto, Hiromichi Naito, Hiromi Ihoriya, Kohei Tsukahara, Tomoyuki Ota, Toshiyuki Watanabe, Atsunori Nakao
BACKGROUND: Raoultella planticola, a Gram-negative, aerobic bacillus commonly isolated from soil and water, rarely causes invasive infections in humans. Septic shock from R. planticola after burn injury has not been previously reported. CASE PRESENTATION: A 79-year-old male was admitted to the emergency intensive care unit after extensive flame burn injury. He accidently caught fire while burning trash and plunged into a nearby tank filled with contaminated rainwater to extinguish the fire...
May 4, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29716597/hepatitis-e-virus-infection-in-turkey-a-systematic-review
#6
REVIEW
Hakan Leblebicioglu, Resat Ozaras
Hepatitis E virus (HEV), a non-enveloped single stranded RNA virus causes sporadic cases of hepatitis or outbreaks. The disease is generally self-limited although it may cause fulminant hepatitis in pregnant women, elderly, those with underlying chronic hepatitis, immunosuppressed, and transplant recipients. It is transmitted through fecal-oral route and zoonotic transmission. Hepatitis is a main health care problem in Turkey; HBV and HCV prevalences are 4 and 1% respectively. Hepatitis D represents another considerable hepatitis etiology with a prevalence of 5-27%...
May 2, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29609660/ginger-zingiber-officinale-phytochemicals-gingerenone-a-and-shogaol-inhibit-sahppk-molecular-docking-molecular-dynamics-simulations-and-in-vitro-approaches
#7
Shailima Rampogu, Ayoung Baek, Rajesh Goud Gajula, Amir Zeb, Rohit S Bavi, Raj Kumar, Yongseong Kim, Yong Jung Kwon, Keun Woo Lee
BACKGROUND: Antibiotic resistance is a defense mechanism, harbored by pathogens to survive under unfavorable conditions. Among several antibiotic resistant microbial consortium, Staphylococcus aureus is one of the most havoc microorganisms. Staphylococcus aureus encodes a unique enzyme 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (SaHPPK), against which, none of existing antibiotics have been reported. METHODS: Computational approaches have been instrumental in designing and discovering new drugs for several diseases...
April 2, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29571295/comparison-of-nephrotoxicity-of-colistin-with-polymyxin-b-administered-in-currently-recommended-doses-a-prospective-study
#8
Ritesh Aggarwal, Arun Dewan
BACKGROUND: The most important concern with polymyxins (Colistin and Polymyxin B) use is nephrotoxicity. There is no prospective data comparing nephrotoxicity of these two drugs, when administered in high doses and as per current recommendations. We conducted a prospective study to compare their trend of nephrotoxicity in our patient population. METHODS: Our study included adult ICU patients who received more than 48 h of Colistin or Polymyxin B and had no confounding factors for nephrotoxicity...
March 23, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29571294/detection-of-neisseria-meningitidis-in-a-paediatric-patient-with-septic-arthritis-using-multiplexed-diagnostic-pcr-targeting-meningitis-encephalitis-me
#9
Donnchadh O'Sullivan, Barry Linnane, Amanda Mostyn, Nteimam Jonathan, Marie Lenihan, Nuala H O'Connell, Colum P Dunne
BACKGROUND: Neisseria meningitidis is associated with meningitis and septicemia. Septic meningococcal arthritis is relatively uncommon and its diagnosis associated with clinical and microbiological challenges. Early recognition and treatment is required to prevent joint destruction. PURPOSE: We describe a case of an eleven-year-old boy with septic arthritis and the first reported use of a multiplexed diagnostic PCR test, capable of simultaneous rapid detection of 14 pathogens directly from CSF samples, to determine presence of N...
March 23, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29571292/genetic-contexts-related-to-the-diffusion-of-plasmid-mediated-ctx-m-55-extended-spectrum-beta-lactamase-isolated-from-enterobacteriaceae-in-china
#10
Xiaoxin Hu, Jianjun Gou, Xiaobing Guo, Zaiqiu Cao, Yuan Li, Hongjian Jiao, Xiaohong He, Yihui Ren, Fuyun Tian
BACKGROUND: CTX-M-55 extended-spectrum beta-lactamases are being rapidly disseminated and transmitted in clinical practices around the world. The genetic contexts of the transferable plasmid-mediated blaCTX-M-55 gene in Enterobacteriaceae were detected and characterized in this study. METHODS: Isolates were obtained from the First Affiliated Hospital of Zhengzhou University between September 2015 and March 2016. Based on polymerase chain reaction and BLAST analysis, resistance genes and genetic context of the blaCTX-M-55 gene were investigated...
March 23, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29571291/factors-associated-to-prevalence-and-treatment-of-carbapenem-resistant-enterobacteriaceae-infections-a-seven-years-retrospective-study-in-three-tertiary-care-hospitals
#11
Feng Pang, Xiu-Qin Jia, Qi-Gang Zhao, Yi Zhang
BACKGROUND: The increasing incidence of carbapenem-resistant Enterobacteriaceae (CRE), has resulted in a difficult problem in the current clinical anti-infective treatment. We performed a retrospective analysis of prevalence and treatment for CRE infections patients. METHODS: This study was conducted in three tertiary care hospitals from January 1, 2010 to December 30, 2016. Baseline data, treatment, and outcomes were collected in patients with ventilator-associated bacterial pneumonia (VABP), bacteremia, complicated urinary tract infection (cUTI)/acute pyelonephritis (AP), hospital-acquired bacterial pneumonia (HABP), superficial wound infection (SWI), biliary tract infection (BTI), deep wound infection (DWI) and sterile body fluids infection (SBFI) due to CRE...
March 23, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29566704/a-case-of-cardiobacterium-valvarum-endocarditis-with-cerebral-hemorrhage-after-mvr-tvp-and-vegetation-removal-operation
#12
Lijia Ni, Xiaoying Xie, Nengyong Ouyang, Baiji Chen, Dongye Wang, Xiaoqiang Liu, Xiquan Wu, Jiajian Guo, Hongyu Li, Yandan Yao, Songyin Huang
BACKGROUND: Cardiobacterium is a fastidious Gram-negative bacillus, and is a rare human pathogen in clinical settings. Herein, we describe a case of Cardiobacterium valvarum (C. valvarum) endocarditis with a rare complication of cerebral hemorrhage after mitral valve replacement (MVR), tricuspid valve prosthesis (TVP) and vegetation removal operation. CASE PRESENTATION: A 41-year-old woman who had a history of gingivitis developed into infective endocarditis due to the infection of C...
March 22, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29562911/application-of-next-generation-sequencing-to-characterize-novel-mutations-in-clarithromycin-susceptible-helicobacter-pylori-strains-with-a2143g-of-23s-rrna-gene
#13
Jiaoe Chen, Liping Ye, Liangmin Jin, Xuehua Xu, Peisong Xu, Xianjun Wang, Hongzhang Li
BACKGROUND: Clarithromycin (CLR) resistance has become a predominant factor for treatment failure of Helicobacter pylori eradication. Although the molecular mechanism of CLR resistance has been clearly understood in H. pylori, it is lack of evidence of other genes involved in drug resistance. Furthermore, the molecular mechanism of phenotype susceptible to CLR while genotype of 23S rRNA is mutant with A2143G is unclear. Here, we characterized the mutations of CLR-resistant and -susceptible H...
March 22, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29554907/prenatal-and-postnatal-antibiotic-exposure-influences-the-gut-microbiota-of-preterm-infants-in-neonatal-intensive-care-units
#14
Zhi-Hui Zou, Dong Liu, Hong-Dong Li, Dan-Ping Zhu, Yu He, Ting Hou, Jia-Lin Yu
BACKGROUND: To explore the influences of prenatal antibiotic exposure, the intensity of prenatal and postnatal antibiotic exposure on gut microbiota of preterm infants and whether gut microbiota and drug resistant strains in the neonatal intensive care unit (NICU) over a defined period are related. METHODS: Among 28 preterm infants, there were two groups, the PAT (prenatal antibiotic therapy) group (12 cases), and the PAF (prenatal antibiotic free) group (12 cases)...
March 19, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29544544/comparative-and-molecular-analysis-of-mrsa-isolates-from-infection-sites-and-carrier-colonization-sites
#15
Khaled R Alkharsah, Suriya Rehman, Fatimah Alkhamis, Amani Alnimr, Asim Diab, Amein K Al-Ali
BACKGROUND: Methicillin resistant Staphylococcus aureus (MRSA) constitutes a major global health concern causing hospital and community acquired infections. A wide diversity of MRSA genotypes are circulating in geographically related regions. Therefore understanding the molecular epidemiology of MRSA is fundamental to design control and clearance measures. METHODS: A total of 106 MRSA isolates from infection (51) and carrier colonization sites (55) are characterized genetically based on SCCmec and MLST genotyping methods in addition to detection of PVL, TSST-1 and enterotoxins...
March 15, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29544479/quality-control-of-malaria-microscopy-reveals-misdiagnosed-non-falciparum-species-and-other-microscopically-detectable-pathogens-in-senegal
#16
Mamadou Alpha Diallo, Khadim Diongue, Mame Cheikh Seck, Mouhamadou Ndiaye, Ibrahima Diallo, Younouss Diedhiou, Tolla Ndiaye, Yaye Die Ndiaye, Aida Sadikh Badiane, Daouda Ndiaye
BACKGROUND: In developing countries, malaria diagnosis relies on microscopy and rapid diagnostic tests. In Senegal, national malaria control program (NMCP) regularly conducts supervisory visits in health services where malaria microscopy is performed. In this study, expert microscopists assessed the performance of laboratory technicians in malaria microscopy. METHODS: The present external quality assessment (EQA) was conducted in three different areas of malaria transmission...
March 15, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29471819/dengue-periodic-outbreaks-and-epidemiological-trends-in-nepal
#17
REVIEW
Birendra Prasad Gupta, Reshma Tuladhar, Roshan Kurmi, Krishna Das Manandhar
Dengue is a global health problem and expansion of its endemics towards new territories in the hilly regions in Nepal is a serious concern. It appeared as a new disease in Nepal in 2004 from Japanese traveler with sporadic cases every year and massive outbreaks in 2010, 2013 and 2016. The serotype was responsible for outbreak in particular year was dengue virus serotype-1 (DENV-1) in 2010, 2016; and DENV-2 in 2013. Nepal lacks basic health related infrastructure in rural areas and does not have a stringent health care policy...
February 23, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29463249/continued-in-vitro-cefazolin-susceptibility-in-methicillin-susceptible-staphylococcus-aureus
#18
Benjamin H Gern, Alexander L Greninger, Scott J Weissman, Jennifer R Stapp, Yue Tao, Xuan Qin
OBJECTIVES: In vitro trends of cefazolin and ceftriaxone susceptibilities from pediatric clinical isolates of methicillin-susceptible Staphylococcus aureus (MSSA) between 2011 and 2016 were analyzed for surveillance. METHODS: Our laboratory continues to use agar disk diffusion for staphylococcal susceptibilities applying Clinical Laboratory Standard Institute's 2012 breakpoints. RESULTS: A total of 3992 MSSA clinical isolates in the last 6 years were analyzed for their in vitro cefazolin and ceftriaxone susceptibilities...
February 20, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29433582/the-frequency-of-klebsiella-pneumonia-encoding-genes-for-ctx-m-tem-1-and-shv-1-extended-spectrum-beta-lactamases-enzymes-isolated-from-urinary-tract-infection
#19
Masomeh Dehshiri, Seyed Sajjad Khoramrooz, Mohammad Zoladl, Seyed Abdolmajid Khosravani, Najmeh Parhizgari, Mohammad Hossein Motazedian, Soheyla Jahedi, Asghar Sharifi
BACKGROUND: The extended- spectrum β-lactamase producing bacteria are widely spread worldwide. The productions of these enzymes cause bacterial resistance to a wide range of antibiotics. The aim of this study was to investigated the frequency of K. pneumonia encoding genes for CTX-M, TEM-1 and SHV-1 extended-spectrum beta lactamases enzymes isolated from urinary tract infection. METHODS: This study is cross-sectional study. All K. pneumonia isolates from urine samples, which had grown on media culture more than 105 were delivered to the medical microbiology laboratory...
February 13, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29422058/an-evaluation-of-e-coli-in-urinary-tract-infection-in-emergency-department-at-kamc-in-riyadh-saudi-arabia-retrospective-study
#20
Menyfah Q Alanazi, Fulwah Y Alqahtani, Fadilah S Aleanizy
BACKGROUND: Urinary tract infection (UTIS) is a common infectious disease in which level of antimicrobial resistance are alarming worldwide. Therefore, this study aims to describe the prevalence and the resistance pattern of the main bacteria responsible for UTIS Escherichia coli (E. coli). METHODS: Retrospective chart review for patients admitted to emergency department and diagnosed with UTIS at KAMC, in Riyadh, Saudi Arabia between January to March 2008 was performed...
February 9, 2018: Annals of Clinical Microbiology and Antimicrobials
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