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Annals of Clinical Microbiology and Antimicrobials

Melissa Barreto Falcao, Sergio Cimerman, Kleber Giovanni Luz, Alberto Chebabo, Helena Andrade Brigido, Iza Maria Lobo, Artur Timerman, Rodrigo Nogueira Angerami, Clovis Arns da Cunha, Helio Arthur Bacha, Jesse Reis Alves, Alexandre Naime Barbosa, Ralcyon Francis Teixeira, Leonardo Weissmann, Priscila Rosalba Oliveira, Marco Antonio Cyrillo, Antonio Carlos Bandeira
A panel of national experts was convened by the Brazilian Infectious Diseases Society in order to organize the national recommendations for the management of zika virus infection. The focus of this document is the diagnosis, both clinical and laboratorial, and appropriate treatment of the diverse manifestations of this infection, ranging from acute mild disease to Guillain-Barré syndrome and also microcephaly and congenital malformations.
September 29, 2016: Annals of Clinical Microbiology and Antimicrobials
Jun Li, Mingxiang Zou, Qingya Dou, Yongmei Hu, Haichen Wang, Qun Yan, Wen' En Liu
BACKGROUND: Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011-2012 at nine hospitals in the Hunan province of China. METHODS: Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR)...
May 23, 2016: Annals of Clinical Microbiology and Antimicrobials
Agila K Pragasam, M Raghanivedha, Shalini Anandan, Balaji Veeraraghavan
Pseudomonas aeruginosa is the most common nosocomial pathogen, notorious for its multidrug resistance and causes life threatening infections. Carbapenems were considered as the last resort of drugs for the treatment of multi drug resistant P. aeruginosa infections. The emergence of resistance to carbapenems limits its use for treatment. Unlike other organisms, in P. aeruginosa intrinsic/chromosomal mediated resistance mechanisms plays a major role for carbapenem resistance rather than the carbapenemases. Carbapenemase producing organisms becomes resistant to both imipenem and meropenem...
February 24, 2016: Annals of Clinical Microbiology and Antimicrobials
Waleed M Sweileh, Naser Y Shraim, Samah W Al-Jabi, Ansam F Sawalha, Adham S AbuTaha, Sa'ed H Zyoud
BACKGROUND: Antimicrobial resistance is a global public health challenge and carbapenem resistance, in particular, is considered an urgent global health threat. This study was carried out to give a bibliometric overview of literature on carbapenem resistance. In specific, number of publications, top productive countries and institutes, highly cited articles, citation analysis, co-authorships, international collaboration, top active authors, and journals publishing articles on carbapenem resistance were analyzed and discussed...
2016: Annals of Clinical Microbiology and Antimicrobials
Alexander Rohde, Jens Andre Hammerl, Sascha Al Dahouk
BACKGROUND: The emerging threat posed by antibiotic resistance has affected public health systems all over the world. Surveillance of resistant bacteria in clinical settings and identifying them in mixed cultures is of paramount importance and can contribute to the control of their spreading. Culture-independent monitoring approaches are highly desirable, since they yield results much faster than traditional susceptibility testing. However, many rapid molecular methods like PCR only detect the sole presence of a potential resistance gene, do not provide information regarding efficient transcription, expression and functionality and, in addition, cannot assign resistance genes to species level in mixed cultures...
2016: Annals of Clinical Microbiology and Antimicrobials
Eleanor A Powell, Joel E Mortensen
The BioMerieux NucliSENS easyMAG total nucleic acid extractor was evaluated for use on bacterial isolates in the clinical microbiology laboratory. Forty eight isolates were extracted, yielding quantifiable amounts of DNA for all isolates. The easyMAG is appropriate for DNA extraction from bacterial isolates and will be incorporated in the clinical laboratory.
2016: Annals of Clinical Microbiology and Antimicrobials
Geoffrey Omuse, Kristien Nel Van Zyl, Kim Hoek, Shima Abdulgader, Samuel Kariuki, Andrew Whitelaw, Gunturu Revathi
BACKGROUND: Staphylococcus aureus (S. aureus) has established itself over the years as a major cause of morbidity and mortality both within the community and in healthcare settings. Methicillin resistant S. aureus (MRSA) in particular has been a major cause of nosocomial infections resulting in significant increase in healthcare costs. In Africa, the MRSA prevalence has been shown to vary across different countries. In order to better understand the epidemiology of MRSA in a setting, it is important to define its population structure using molecular tools as different clones have been found to predominate in certain geographical locations...
2016: Annals of Clinical Microbiology and Antimicrobials
Akash Tariq, Muhammad Adnan, Rahila Amber, Kaiwen Pan, Sakina Mussarat, Zabta Khan Shinwari
BACKGROUND: Leishmaniasis and malaria are the two most common parasitic diseases and responsible for large number of deaths per year particularly in developing countries like Pakistan. Majority of Pakistan population rely on medicinal plants due to their low socio-economic status. The present review was designed to gather utmost fragmented published data on traditionally used medicinal plants against leishmaniasis and malaria in Pakistan and their scientific validation. METHODS: Pub Med, Google Scholar, Web of Science, ISI Web of knowledge and Flora of Pakistan were searched for the collection of data on ethnomedicinal plants...
2016: Annals of Clinical Microbiology and Antimicrobials
Alexandra Brunner, Eva Nemes-Nikodem, Csaba Jeney, Dora Szabo, Marta Marschalko, Sarolta Karpati, Eszter Ostorhazi
BACKGROUND: In the 1990s, azithromycin became the drug of choice for many infectious diseases but emerging resistance to the drug has only been reported in the last decade. In the last 5 years, the National Neisseria gonorrhoeae Reference Laboratory of Hungary (NNGRLH) has also observed an increased number of N. gonorrhoeae strains resistant to azithromycin. The aim of this study was to determine the most frequent sequence types (ST) of N. gonorrhoeae related to elevated levels of azithromycin MIC (minimal inhibitory concentration)...
2016: Annals of Clinical Microbiology and Antimicrobials
Tsedale Semunigus, Belay Tessema, Setegn Eshetie, Feleke Moges
BACKGROUND: Tuberculosis (TB) remains one of the globe's deadliest communicable diseases. The homeless individuals are at high risk to acquire TB and multi-drug resistant TB (MDR-TB), because of their poor living conditions and risky behaviors. Tuberculosis and MDR-TB in the homeless individuals can pose a risk to entire communities. However, the magnitude of the problem is not known in Ethiopia. Therefore, the aim of this study was to determine the prevalence and associated factors of smear positive pulmonary TB (PTB) and MDR-TB among homeless individuals in Dessie and Debre Birhan towns, Northeast Ethiopia...
2016: Annals of Clinical Microbiology and Antimicrobials
Noah Obeng-Nkrumah, Appiah-Korang Labi, Naa Okaikor Addison, Juliana Ewuramma Mbiriba Labi, Georgina Awuah-Mensah
BACKGROUND: Bloodstream infections (BSI) are life-threatening emergencies. Identification of the common pathogens and their susceptibility patterns is necessary for timely empirical intervention. METHODS: We conducted a 4-year retrospective analysis of blood cultures from all patients excluding neonates at the Korle-Bu Teaching hospital, Ghana, from January 2010 through December 2013. Laboratory report data were used to determine BSI, blood culture contamination, pathogen profile, and antimicrobial resistance patterns...
2016: Annals of Clinical Microbiology and Antimicrobials
Fatma Elzahraa Akram, Tarek El-Tayeb, Khaled Abou-Aisha, Mohamed El-Azizi
BACKGROUND: Silver nanoparticles (AgNPs) are potential antimicrobials agents, which can be considered as an alternative to antibiotics for the treatment of infections caused by multi-drug resistant bacteria. The antimicrobial effects of double and triple combinations of AgNPs, visible blue light, and the conventional antibiotics amoxicillin, azithromycin, clarithromycin, linezolid, and vancomycin, against ten clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were investigated...
2016: Annals of Clinical Microbiology and Antimicrobials
Dietmar Enko, Gabriele Halwachs-Baumann, Robert Stolba, Ortrun Rössler, Gernot Kriegshäuser
BACKGROUND: A growing number of rapid Helicobacter pylori antibody tests are commercially available now, however, some of these tests are often used without sufficient evaluation. The aim of this study was to evaluate the performance of a commercially available rapid whole-blood immunoassay (gabControl(®) H. pylori; gabmed GmbH, Köln, Germany), for the qualitative detection of IgG antibodies against H. pylori with the (13)C-urea breath test ((13)C-UBT) serving as a reference method...
2016: Annals of Clinical Microbiology and Antimicrobials
Mustafa Kolukirik, Mesut Yılmaz, Orhan Ince, Canan Ketre, Ayşe Istanbullu Tosun, Bahar K Ince
BACKGROUND: Group A streptococci (GAS) are the most common bacterial cause of acute pharyngitis and account for 15-30 % of cases of acute pharyngitis in children and 5-10 % of cases in adults. In this study, a real-time quantitative PCR (qPCR) based GAS detection assay in pharyngeal swab specimens was developed. METHODS: The qPCR assay was compared with the gold standard bacterial culture and a rapid antigen detection test (RADT) to evaluate its clinical performance in 687 patients...
2016: Annals of Clinical Microbiology and Antimicrobials
Aydir Cecília Marinho Monteiro, Carlos Magno Castelo Branco Fortaleza, Adriano Martison Ferreira, Ricardo de Souza Cavalcante, Alessandro Lia Mondelli, Eduardo Bagagli, Maria de Lourdes Ribeiro de Souza da Cunha
BACKGROUND: Bloodstream infections are responsible for thousands of deaths each year. The rapid identification of the microorganisms causing these infections permits correct therapeutic management that will improve the prognosis of the patient. In an attempt to reduce the time spent on this step, microorganism identification devices have been developed, including the VITEK(®) 2 system, which is currently used in routine clinical microbiology laboratories. METHODS: This study evaluated the accuracy of the VITEK(®) 2 system in the identification of 400 microorganisms isolated from blood cultures and compared the results to those obtained with conventional phenotypic and genotypic methods...
2016: Annals of Clinical Microbiology and Antimicrobials
Hakan Leblebicioglu
No abstract text is available yet for this article.
2016: Annals of Clinical Microbiology and Antimicrobials
Kata R Mikuláss, Krisztina Nagy, Balázs Bogos, Zsolt Szegletes, Etelka Kovács, Attila Farkas, György Váró, Éva Kondorosi, Attila Kereszt
BACKGROUND: Certain legume plants produce a plethora of AMP-like peptides in their symbiotic cells. The cationic subgroup of the nodule-specific cysteine-rich (NCR) peptides has potent antimicrobial activity against gram-negative and gram-positive bacteria as well as unicellular and filamentous fungi. FINDINGS: It was shown by scanning and atomic force microscopies that the cationic peptides NCR335, NCR247 and Polymyxin B (PMB) affect differentially on the surfaces of Sinorhizobium meliloti bacteria...
2016: Annals of Clinical Microbiology and Antimicrobials
Alberto E Paniz-Mondolfi, Alfonso J Rodriguez-Morales, Gabriela Blohm, Marilianna Marquez, Wilmer E Villamil-Gomez
No abstract text is available yet for this article.
2016: Annals of Clinical Microbiology and Antimicrobials
Akosua Adom Agyeman, Richard Ofori-Asenso
BACKGROUND: Treatment options for drug-resistant tuberculosis are still limited. Linezolid has been recommended for treatment of patients with multidrug-resistant (MDR) or extensively-drug-resistant (XDR) tuberculosis, although uncertainties remain regarding its safety and tolerability in these circumstances. OBJECTIVE: To systematically evaluate the existing evidence regarding the efficacy and tolerability of linezolid in the treatment of MDR or XDR tuberculosis...
2016: Annals of Clinical Microbiology and Antimicrobials
Qingwen He, Weiyuan Chen, Liya Huang, Qili Lin, Jingling Zhang, Rui Liu, Bin Li
BACKGROUND: Carbapenem-resistant Enterobacteriaceae (CRE) is prevalent around the world. Rapid and accurate detection of CRE is urgently needed to provide effective treatment. Automated identification systems have been widely used in clinical microbiology laboratories for rapid and high-efficient identification of pathogenic bacteria. However, critical evaluation and comparison are needed to determine the specificity and accuracy of different systems. The aim of this study was to evaluate the performance of three commonly used automated identification systems on the detection of CRE...
2016: Annals of Clinical Microbiology and Antimicrobials
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