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Assay and Drug Development Technologies

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https://www.readbyqxmd.com/read/28322599/dissolvit-an-in-vitro-method-for-simulating-the-dissolution-and-absorption-of-inhaled-dry-powder-drugs-in-the-lungs
#1
Per Gerde, Maria Malmlöf, Lina Havsborn, Carl-Olof Sjöberg, Pär Ewing, Stefan Eirefelt, Katarina Ekelund
The main purpose of this work was to develop an in vitro method for simulating the dissolution and absorption of inhaled dry powder drugs that also mimics systemic pharmacokinetic data. A second purpose was to evaluate this method. DissolvIt(®) was developed as a simulation of the air-blood barrier of the upper airways, constituting: "airborne" particles deposited on a glass cover slip, a mucus simulant, a polycarbonate (basal) membrane, and a pumped albumin buffer simulating the pulmonary blood flow. The PreciseInhale(®) exposure system was used to aerosolize and deposit test formulations onto cover slips...
February 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28322598/screening-and-functional-profiling-of-small-molecule-hiv-1-entry-and-fusion-inhibitors
#2
Charline Giroud, Yuhong Du, Mariana Marin, Qui Min, Nathan T Jui, Haian Fu, Gregory B Melikyan
HIV-1 entry and fusion with target cells is an important target for antiviral therapy. However, a few currently approved treatments are not effective as monotherapy due to the emergence of drug resistance. This consideration has fueled efforts to develop new bioavailable inhibitors targeting different steps of the HIV-1 entry process. Here, a high-throughput screen was performed of a large library of 100,000 small molecules for HIV-1 entry/fusion inhibitors, using a direct virus-cell fusion assay in a 384 half-well format...
February 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27996302/determination-of-interference-during-in-vitro-pyrogen-detection-development-and-characterization-of-a-cell-based-assay
#3
Linda Palma, Francesca Rossetti, Sabrina Dominici, Costantina Buondelmonte, Marco B L Rocchi, Gian P Rizzardi, Giuliana Vallanti, Mauro Magnani
Contamination of pharmaceutical products and medical devices with pyrogens such as endotoxins is the most common cause of systemic inflammation and, in worst cases, of septic shock. Thus, quantification of pyrogens is crucial. The limulus amebocyte lysate (LAL)-based assays are the reference tests for in vitro endotoxin detection, in association with the in vivo rabbit pyrogen test (RPT), according to European Pharmacopoeia (EP 2.6.14), and U.S. Pharmacopoeia (USP <85>). However, several substances interfere with LAL assay, while RPT is not accurate, not quantitative, and raises ethical limits...
February 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28092461/sbi-2-hcs-hca-3d-imaging-best-practices-and-unmet-needs-colloquium
#4
Ann F Hoffman, Kaylene J Simpson, Peter Horvath, Carrie Lovitt, Serena Silver, Evan Easton, Daniel V LaBarbera, Melissa Mendez, Mark E Rothenberg, Jan Seldin, Judi Wardwell-Swanson, Myles Fennell
No abstract text is available yet for this article.
January 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28092460/a-three-dimensional-lymphatic-endothelial-cell-tube-formation-assay-to-identify-novel-kinases-involved-in-lymphatic-vessel-remodeling
#5
T Jessica Gambino, Steven P Williams, Carol Caesar, Daniel Resnick, Cameron J Nowell, Rae H Farnsworth, Marc G Achen, Steven A Stacker, Tara Karnezis
The lymphatic system is a series of vessels that transport cells and excess fluid from tissues to the blood vascular system. Normally quiescent, the lymphatics can grow or remodel in response to developmental, immunological, or cells pathological stimuli. Lymphatic vessels comprise lymphatic endothelial cells (LECs) that can respond to external growth factors by undergoing proliferation, migration, adhesion, and tube and lumen formation into new vessel structures, a process known as lymphangiogenesis. To understand the key gene and signaling pathways necessary for lymphangiogenesis and lymphatic vessel remodeling, we have developed a three-dimensional LEC tube formation assay to explore the role of kinase signaling in these processes...
January 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28092459/toward-high-throughput-and-multiplexed-imaging-of-genome-organization
#6
Eric F Joyce
Dr. Eric Joyce from the Department of Genetics at the University of Pennsylvania was awarded The President's Innovation award at the annual Society of Biomolecular Imaging and Informatics meeting held in Boston, September 2016. Chromosome interactions are a fundamental aspect of nuclear organization that can activate and silence genes or even direct chromosome rearrangements. However, the molecular mechanisms underlying how chromosomal segments find each other and form stable interactions within cells remain unknown...
January 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28005393/multiplexed-single-cell-imaging-past-present-and-future
#7
Jia-Ren Lin
Jia-Ren Lin from the Laboratory of Systems Pharmacology at Harvard Medical School was awarded best poster at the annual Society of Biomolecular Imaging and Informatics meeting held in Boston, September 2016. His work focuses on single-cell imaging, especially on developing new methods for simultaneously detecting many antigens, named cyclic immunofluorescence (CycIF). This method could be applied in different stages of drug development, from discovery phase, preclinical research to clinical research. The current works and future directions of CycIF method are summarized in the following overview...
January 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27788017/effect-size-measures-as-descriptors-of-assay-quality-in-high-content-screening-a-brief-review-of-some-available-methodologies
#8
Bartek Rajwa
The field of high-content screening (HCS) typically uses measures of screen quality conceived for fairly straightforward high-throughput screening (HTS) scenarios. However, in contrast to HTS, image-based HCS systems rely on multidimensional readouts reporting biological responses associated with complex cellular phenotypes. Not only is the dimensionality in which the screens operate higher, but also the scale of the individual features describing the quantified phenotypic changes is often smaller than what is seen in one-dimensional HTS platforms...
January 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27982706/drug-repurposing-patent-applications-april-june-2016
#9
Hermann A M Mucke
No abstract text is available yet for this article.
December 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27982705/drug-repurposing-patent-applications-july-september-2016
#10
Hermann A M Mucke
No abstract text is available yet for this article.
December 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27982704/tramadol-an-opioid-receptor-agonist-an-inhibitor-of-growth-morphogenesis-and-biofilm-formation-in-the-human-pathogen-candida-albicans
#11
Gunderao Hanumantrao Kathwate, S Mohan Karuppayil
Tramadol is a synthetic, centrally acting low-affinity agonist of μ-opioid receptors in humans. It is used as an analgesic and is shown to have local anesthetic action. In this study, we have tried to explore its anti-Candida potential. Minimum inhibitory concentration (MIC50) and minimum fungicidal concentration (MFC) values were established. MIC50 ranged from 2 to 4 mg/mL, whereas MFC was recorded at 8 mg/mL. Also, the effect of tramadol on germ tube formation, adhesion, and biofilms in Candida albicans was studied...
December 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27982703/revisiting-repurposing
#12
Elizabeth R Sharlow
Drug repurposing can be a cost-effective strategy to identify new small molecule-based therapies. Thus, drug repurposing significantly influences the discovery of therapeutics, particularly for rare and neglected diseases, which are often constrained by limited research and development funds. The push for translational science and access to drug discovery-associated resources such as high throughput screening instrumentation, assay development expertise, and Food and Drug Administration-approved drug libraries have intensified interest in drug repurposing...
December 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27922743/interview-with-farid-khan-phd
#13
(no author information available yet)
No abstract text is available yet for this article.
December 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27631620/big-data-mining-and-adverse-event-pattern-analysis-in-clinical-drug-trials
#14
Callie Federer, Minjae Yoo, Aik Choon Tan
Drug adverse events (AEs) are a major health threat to patients seeking medical treatment and a significant barrier in drug discovery and development. AEs are now required to be submitted during clinical trials and can be extracted from ClinicalTrials.gov ( https://clinicaltrials.gov/ ), a database of clinical studies around the world. By extracting drug and AE information from ClinicalTrials.gov and structuring it into a database, drug-AEs could be established for future drug development and repositioning...
December 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27805424/accurate-determination-of-soluble-axl-by-enzyme-linked-immunosorbent-assay
#15
Mirko Dengler, Heidemarie Huber, Christian J Müller, Angela Zellmer, Peter Rauch, Wolfgang Mikulits
Levels of soluble Axl (sAxl) are routinely assessed in human sera by sandwich enzyme-linked immunosorbent assay (ELISA). Although sAxl values are suggested to diagnose different types of disorders, no uniform ELISA method is available, allowing the reliable interassay comparison between results. Furthermore, little is known about the stability of sAxl under storage conditions, which is a relevant parameter for biomedical trials. The evaluation of sAxl stability under various stress conditions and the determination of proper conditions to use the sAxl ELISA for routine clinical applications are of great interest...
November 2, 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27805422/literature-search-and-review
#16
Mindy I Davis, Anton Simeonov, Doug Auld
No abstract text is available yet for this article.
November 2, 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27845849/an-experimental-model-for-the-rapid-screening-of-compounds-with-potential-use-against-mycobacteria
#17
Sofia Santos Costa, Elizeth Lopes, Elisa Azzali, Diana Machado, Tatiane Coelho, Pedro Eduardo Almeida da Silva, Miguel Viveiros, Marco Pieroni, Isabel Couto
Infections caused by Mycobacterium tuberculosis and other mycobacteria are major challenges for global public health. Particularly worrisome are infections caused by multidrug-resistant bacteria, which are increasingly difficult to treat because of the loss of efficacy of the current antibacterial agents, a problem that continues to escalate worldwide. There has been a limited interest and investment on the development of new antibacterial agents in the past decades. This has led to the current situation, in which there is an urgent demand for innovative therapeutic alternatives to fight infections caused by multidrug-resistant pathogens, such as multidrug-resistant tuberculosis...
November 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27845848/interview-with-doug-auld-phd
#18
(no author information available yet)
No abstract text is available yet for this article.
November 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27801595/analytical-application-of-flow-immunosensor-in-detection-of-thyroxine-and-triiodothyronine-in-serum
#19
Tanveer A Wani, Seema Zargar, Salma Majid, Ibrahim A Darwish
In this study, an immunosensor based on kinetic exclusion analysis (KinExA) was used for thyroxine (T4) and triiodothyronine (T3) estimation. A KinExA™ 3200 instrument was used for this analysis, which is an automated flow fluorimeter designed to separate free unbound antibody binding sites in reaction mixtures of antibody, antigen, and antibody-antigen complex. A T3-BSA- and T4-BSA-coated polymethyl methacrylate (PMMA) bead microcolumn is generated inside the flow cell of the instrument. A sample mixture containing T3 and T4 with their respective monoclonal antibodies and their complexes are drawn past the microbead column...
November 1, 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27732065/author-index-by-page-number
#20
(no author information available yet)
No abstract text is available yet for this article.
October 2016: Assay and Drug Development Technologies
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