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Assay and Drug Development Technologies

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https://www.readbyqxmd.com/read/27805424/accurate-determination-of-soluble-axl-by-enzyme-linked-immunosorbent-assay
#1
Mirko Dengler, Heidemarie Huber, Christian J Müller, Angela Zellmer, Peter Rauch, Wolfgang Mikulits
Levels of soluble Axl (sAxl) are routinely assessed in human sera by sandwich enzyme-linked immunosorbent assay (ELISA). Although sAxl values are suggested to diagnose different types of disorders, no uniform ELISA method is available, allowing the reliable interassay comparison between results. Furthermore, little is known about the stability of sAxl under storage conditions, which is a relevant parameter for biomedical trials. The evaluation of sAxl stability under various stress conditions and the determination of proper conditions to use the sAxl ELISA for routine clinical applications are of great interest...
November 2, 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27805422/literature-search-and-review
#2
Mindy I Davis, Anton Simeonov, Doug Auld
No abstract text is available yet for this article.
November 2, 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27801595/analytical-application-of-flow-immunosensor-in-detection-of-thyroxine-and-triiodothyronine-in-serum
#3
Tanveer A Wani, Seema Zargar, Salma Majid, Ibrahim A Darwish
In this study, an immunosensor based on kinetic exclusion analysis (KinExA) was used for thyroxine (T4) and triiodothyronine (T3) estimation. A KinExA™ 3200 instrument was used for this analysis, which is an automated flow fluorimeter designed to separate free unbound antibody binding sites in reaction mixtures of antibody, antigen, and antibody-antigen complex. A T3-BSA- and T4-BSA-coated polymethyl methacrylate (PMMA) bead microcolumn is generated inside the flow cell of the instrument. A sample mixture containing T3 and T4 with their respective monoclonal antibodies and their complexes are drawn past the microbead column...
November 1, 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27788017/effect-size-measures-as-descriptors-of-assay-quality-in-high-content-screening-a-brief-review-of-some-available-methodologies
#4
Bartek Rajwa
The field of high-content screening (HCS) typically uses measures of screen quality conceived for fairly straightforward high-throughput screening (HTS) scenarios. However, in contrast to HTS, image-based HCS systems rely on multidimensional readouts reporting biological responses associated with complex cellular phenotypes. Not only is the dimensionality in which the screens operate higher, but also the scale of the individual features describing the quantified phenotypic changes is often smaller than what is seen in one-dimensional HTS platforms...
October 27, 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27631620/big-data-mining-and-adverse-event-pattern-analysis-in-clinical-drug-trials
#5
Callie Federer, Minjae Yoo, Aik Choon Tan
Drug adverse events (AEs) are a major health threat to patients seeking medical treatment and a significant barrier in drug discovery and development. AEs are now required to be submitted during clinical trials and can be extracted from ClinicalTrials.gov ( https://clinicaltrials.gov/ ), a database of clinical studies around the world. By extracting drug and AE information from ClinicalTrials.gov and structuring it into a database, drug-AEs could be established for future drug development and repositioning...
September 15, 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27845849/an-experimental-model-for-the-rapid-screening-of-compounds-with-potential-use-against-mycobacteria
#6
Sofia Santos Costa, Elizeth Lopes, Elisa Azzali, Diana Machado, Tatiane Coelho, Pedro Eduardo Almeida da Silva, Miguel Viveiros, Marco Pieroni, Isabel Couto
Infections caused by Mycobacterium tuberculosis and other mycobacteria are major challenges for global public health. Particularly worrisome are infections caused by multidrug-resistant bacteria, which are increasingly difficult to treat because of the loss of efficacy of the current antibacterial agents, a problem that continues to escalate worldwide. There has been a limited interest and investment on the development of new antibacterial agents in the past decades. This has led to the current situation, in which there is an urgent demand for innovative therapeutic alternatives to fight infections caused by multidrug-resistant pathogens, such as multidrug-resistant tuberculosis...
November 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27845848/interview-with-doug-auld-phd
#7
(no author information available yet)
No abstract text is available yet for this article.
November 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27732065/author-index-by-page-number
#8
(no author information available yet)
No abstract text is available yet for this article.
October 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27732064/discovery-of-small-molecules-that-induce-lysosomal-cell-death-in-cancer-cell-lines-using-an-image-based-screening-platform
#9
Romina J Pagliero, Diego S D'Astolfo, Daphne Lelieveld, Riyona D Pratiwi, Sonja Aits, Marja Jaattela, Nathaniel I Martin, Judith Klumperman, David A Egan
The lysosomal cell death (LCD) pathway is a caspase 3-independent cell death pathway that has been suggested as a possible target for cancer therapy, making the development of sensitive and specific high-throughput (HT) assays to identify LCD inducers highly desirable. In this study, we report a two-step HT screening platform to reliably identify such molecules. First, using a robust HT primary screen based on propidium iodide uptake, we identified compounds that kill through nonapoptotic pathways. A phenotypic image-based assay using a galectin-3 (Gal-3) reporter was then used to further classify hits based on lysosomal permeabilization, a hallmark of LCD...
October 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27661290/a-phenotypic-high-content-screening-assay-to-identify-regulators-of-membrane-protein-localization
#10
Lorey K Smith, Daniel W Thomas, Kaylene J Simpson, Patrick O Humbert
Correct subcellular localization of proteins is a requirement for appropriate function. This is especially true in epithelial cells, which rely on the precise localization of a diverse array of epithelial polarity and cellular adhesion proteins. Loss of cell polarity and adhesion is a hallmark of cancer, and mislocalization of core polarity proteins, such as Scribble, is observed in a range of human epithelial tumors and is prognostic of poor survival. Despite this, little is known about how Scribble membrane localization is regulated...
October 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27661136/society-of-biomolecular-imaging-and-informatics-special-issue-october-2016
#11
Myles Fennell, Kaylene J Simpson
No abstract text is available yet for this article.
October 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27636821/hc-stratominer-a-web-based-tool-for-the-rapid-analysis-of-high-content-datasets
#12
Wienand A Omta, Roy G van Heesbeen, Romina J Pagliero, Lieke M van der Velden, Daphne Lelieveld, Mehdi Nellen, Maik Kramer, Marley Yeong, Amir M Saeidi, Rene H Medema, Marco Spruit, Sjaak Brinkkemper, Judith Klumperman, David A Egan
High-content screening (HCS) can generate large multidimensional datasets and when aligned with the appropriate data mining tools, it can yield valuable insights into the mechanism of action of bioactive molecules. However, easy-to-use data mining tools are not widely available, with the result that these datasets are frequently underutilized. Here, we present HC StratoMineR, a web-based tool for high-content data analysis. It is a decision-supportive platform that guides even non-expert users through a high-content data analysis workflow...
October 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27611036/the-society-of-biomolecular-imaging-and-informatics-3rd-annual-conference-high-content-2016-september-12-14-2016-joseph-b-martin-conference-center-harvard-medical-school-boston-ma
#13
(no author information available yet)
No abstract text is available yet for this article.
October 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27606620/reconfiguring-the-ar-tif2-protein-protein-interaction-hcs-assay-in-prostate-cancer-cells-and-characterizing-the-hits-from-a-lopac-screen
#14
Ashley T Fancher, Yun Hua, Daniel P Camarco, David A Close, Christopher J Strock, Paul A Johnston
The continued activation of androgen receptor (AR) transcription and elevated expression of AR and transcriptional intermediary factor 2 (TIF2) coactivator observed in prostate cancer (CaP) recurrence and the development of castration-resistant CaP (CRPC) support a screening strategy for small-molecule inhibitors of AR-TIF2 protein-protein interactions (PPIs) to find new drug candidates. Small molecules can elicit tissue selective effects, because the cells of distinct tissues express different levels and cohorts of coregulatory proteins...
October 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27552146/sbi-2-high-content-2016-3-rd-annual-conference-september-12-th-14-th
#15
(no author information available yet)
No abstract text is available yet for this article.
September 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27552145/combining-high-content-imaging-and-phenotypic-classification-analysis-of-senescence-associated-beta-galactosidase-staining-to-identify-regulators-of-oncogene-induced-senescence
#16
Keefe T Chan, Lassi Paavolainen, Katherine M Hannan, Amee J George, Ross D Hannan, Kaylene J Simpson, Peter Horvath, Richard B Pearson
Hyperactivation of the PI3K/AKT/mTORC1 signaling pathway is a hallmark of the majority of sporadic human cancers. Paradoxically, chronic activation of this pathway in nontransformed cells promotes senescence, which acts as a significant barrier to malignant progression. Understanding how this oncogene-induced senescence is maintained in nontransformed cells and conversely how it is subverted in cancer cells will provide insight into cancer development and potentially identify novel therapeutic targets. High-throughput screening provides a powerful platform for target discovery...
September 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27552144/development-of-the-theta-comparative-cell-scoring-method-to-quantify-diverse-phenotypic-responses-between-distinct-cell-types
#17
Scott J Warchal, John C Dawson, Neil O Carragher
In this article, we have developed novel data visualization tools and a Theta comparative cell scoring (TCCS) method, which supports high-throughput in vitro pharmacogenomic studies across diverse cellular phenotypes measured by multiparametric high-content analysis. The TCCS method provides a univariate descriptor of divergent compound-induced phenotypic responses between distinct cell types, which can be used for correlation with genetic, epigenetic, and proteomic datasets to support the identification of biomarkers and further elucidate drug mechanism-of-action...
September 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27552143/assessing-drug-efficacy-in-a-miniaturized-pancreatic-cancer-in-vitro-3d-cell-culture-model
#18
Todd B Shelper, Carrie J Lovitt, Vicky M Avery
Pancreatic cancer continues to have one of the poorest prognoses among all cancers. The drug discovery efforts for this disease have largely failed, with no significant improvement in survival outcomes for advanced pancreatic cancer patients over the past 20 years. Traditional in vitro cell culture techniques have been used extensively in both basic and early drug discovery; however, these systems offer poor models to assess emerging therapeutics. More predictive cell-based models, which better capture the cellular heterogeneity and complexities of solid pancreatic tumors, are urgently needed not only to improve drug discovery success but also to provide insight into the tumor biology...
September 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27552142/society-of-biomolecular-imaging-and-informatics-special-issue
#19
Myles Fennell, Kaylene J Simpson
No abstract text is available yet for this article.
September 2016: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/27504922/accurate-cytotoxicity-and-proliferation-determination-advantages-of-a-high-throughput-phenotypic-approach-over-atp-luminescence-assays
#20
Anne F Hammerstein, Paul G Wylie
Cell viability and proliferation assays are a fundamental tool in the drug discovery process and are used to evaluate both the antiproliferative potency and toxicity of compounds. Some lead discovery groups generate cell viability data for up to two million compounds per screen, so any method used to assess these parameters needs to deliver not only on data quality but also on throughput and assay cost per well. Most methods used to determine cell viability cannot deliver on all three of these requirements, so compromises have to be made...
September 2016: Assay and Drug Development Technologies
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