journal
MENU ▼
Read by QxMD icon Read
search

Archaea: An International Microbiological Journal

journal
https://www.readbyqxmd.com/read/30228804/soil-microbial-community-structure-and-diversity-around-the-aging-oil-sludge-in-yellow-river-delta-as-determined-by-high-throughput-sequencing
#1
Shaoping Kuang, Yaqing Su, Huihui Wang, Wenjuan Yu, Qiaolin Lang, Ravichandra Matangi
Microorganisms are sensitive indicators of edaphic environmental variation. The Illumina MiSeq sequencing technology was used to analyze soil bacterial community diversity around an aging oil sludge in the Yellow River Delta. The alpha diversity index of soil bacterial community results (Ace, Chao, Shannon, and Simpson) determined that bacterial community diversity sampling within the scope of a 20 cm radius from the center of an aging oil sludge spot showed the most abundant diversity. The level of diversity distributed symmetrically with radial direction from the center of the aging oil sludge spot...
2018: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/30210264/comparative-genomic-analysis-of-members-of-the-genera-methanosphaera-and-methanobrevibacter-reveals-distinct-clades-with-specific-potential-metabolic-functions
#2
Anja Poehlein, Dominik Schneider, Melissa Soh, Rolf Daniel, Henning Seedorf
Methanobrevibacter and Methanosphaera species represent some of the most prevalent methanogenic archaea in the gastrointestinal tract of animals and humans and play an important role in this environment. The aim of this study was to identify genomic features that are shared or specific for members of each genus with a special emphasis of the analysis on the assimilation of nitrogen and acetate and the utilization of methanol and ethanol for methanogenesis. Here, draft genome sequences of Methanobrevibacter thaueri strain DSM 11995T , Methanobrevibacter woesei strain DSM 11979T , and Methanosphaera cuniculi strain 4103T are reported and compared to those of 16 other Methanobrevibacter and Methanosphaera genomes, including genomes of the 13 currently available types of strains of the two genera...
2018: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/30174556/autohydrogenotrophic-denitrification-using-the-membrane-biofilm-reactor-for-removing-nitrate-from-high-sulfate-concentration-of-water
#3
Yanhao Zhang, Haohan Zhang, Zhibin Zhang, Yuchen Wang, Taha Marhaba, Jixiang Li, Cuizhen Sun, Wen Zhang
This study investigated the performance of an autohydrogenotrophic membrane biofilm reactor (MBfR) to remove nitrate from water with high sulfate concentrations. The results of simulated running showed that TN removal could be over than 98.8% with the maximum denitrification rate of 134.6 g N/m3  d under the conditions of the influent sulfate concentrations of 300 mg SO4 2- /l. The distribution ratio of H2 electron donor for nitrate and sulfate was 70.0 : 26.9 at the high influent loading ratio of sulfate/nitrate of 853...
2018: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/30154680/bioaugmentation-with-mixed-hydrogen-producing-acetogen-cultures-enhances-methane-production-in-molasses-wastewater-treatment
#4
Shuo Wang, Jianzheng Li, Guochen Zheng, Guocheng Du, Ji Li
Hydrogen-producing acetogens (HPA) have a transitional role in anaerobic wastewater treatment. Thus, bioaugmentation with HPA cultures can enhance the chemical oxygen demand (COD) removal efficiency and CH4 yield of anaerobic wastewater treatment. Cultures with high degradation capacities for propionic acid and butyric acid were obtained through continuous subculture in enrichment medium and were designated as Z08 and Z12. Bioaugmentation with Z08 and Z12 increased CH4 production by glucose removal to 1.58...
2018: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/30123085/hydrogenotrophic-methanogenesis-and-autotrophic-growth-of-methanosarcina-thermophila
#5
Nina Lackner, Anna Hintersonnleitner, Andreas Otto Wagner, Paul Illmer
Although Methanosarcinales are versatile concerning their methanogenic substrates, the ability of Methanosarcina thermophila to use carbon dioxide (CO2 ) for catabolic and anabolic metabolism was not proven until now. Here, we show that M. thermophila used CO2 to perform hydrogenotrophic methanogenesis in the presence as well as in the absence of methanol. During incubation with hydrogen, the methanogen utilized the substrates methanol and CO2 consecutively, resulting in a biphasic methane production. Growth exclusively from CO2 occurred slowly but reproducibly with concomitant production of biomass, verified by DNA quantification...
2018: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/29853797/dynamics-of-archaeal-and-bacterial-communities-in-response-to-variations-of-hydraulic-retention-time-in-an-integrated-anaerobic-fluidized-bed-membrane-bioreactor-treating-benzothiazole-wastewater
#6
Yue Li, Qi Hu, Da-Wen Gao
An integrated anaerobic fluidized-bed membrane bioreactor (IAFMBR) was investigated to treat synthetic high-strength benzothiazole wastewater (50 mg/L) at a hydraulic retention time (HRT) of 24, 18, and 12 h. The chemical oxygen demand (COD) removal efficiency (from 93.6% to 90.9%), the methane percentage (from 70.9% to 69.27%), and the methane yield (from 0.309 m3 CH4 /kg·CODremoved to 0.316 m3 CH4 /kg·CODremoved ) were not affected by decreasing HRTs. However, it had an adverse effect on membrane fouling (decreasing service period from 5...
2018: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/29853796/characterization-of-microbial-communities-in-pilot-scale-constructed-wetlands-with-salicornia-for-treatment-of-marine-aquaculture-effluents
#7
Xiaona Ma, Xingqiang Song, Xian Li, Songzhe Fu, Meng Li, Ying Liu
Microorganisms play an essential role in the performance of constructed wetlands (CWs) for wastewater treatment. However, there has been limited discussion on the characteristics of microbial communities in CWs for treatment of effluents from marine recirculating aquaculture systems (RAS). This study is aimed at characterizing the microbial communities of pilot-scale CWs with Salicornia bigelovii for treatment of saline wastewater from a land-based Atlantic salmon RAS plant located in Northern China. Illumina high-throughput sequencing was employed to identify the profile of microbial communities of three CWs receiving wastewater under different total ammonia nitrogen (TAN) concentrations...
2018: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/29692683/inorganic-polyphosphate-exopolyphosphatase-and-pho84-like-transporters-may-be-involved-in-copper-resistance-in-metallosphaera-sedula-dsm-5348-t
#8
Matías Rivero, Constanza Torres-Paris, Rodrigo Muñoz, Ricardo Cabrera, Claudio A Navarro, Carlos A Jerez
Polyphosphates (PolyP) are linear polymers of orthophosphate residues that have been proposed to participate in metal resistance in bacteria and archaea. In addition of having a CopA/CopB copper efflux system, the thermoacidophilic archaeon Metallosphaera sedula contains electron-dense PolyP-like granules and a putative exopolyphosphatase (PPX Msed , Msed_0891 ) and four presumed pho84 -like phosphate transporters ( Msed_0846 , Msed_0866 , Msed_1094 , and Msed_1512 ) encoded in its genome. In the present report, the existence of a possible PolyP-based copper-resistance mechanism in M...
2018: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/29391857/community-and-proteomic-analysis-of-anaerobic-consortia-converting-tetramethylammonium-to-methane
#9
Wei-Yu Chen, Lucia Kraková, Jer-Horng Wu, Domenico Pangallo, Lenka Jeszeová, Bing Liu, Hidenari Yasui
Tetramethylammonium-degrading methanogenic consortia from a complete-mixing suspended sludge (CMSS) and an upflow anaerobic sludge blanket (UASB) reactors were studied using multiple PCR-based molecular techniques and shotgun proteomic approach. The prokaryotic 16S rRNA genes of the consortia were analyzed by quantitative PCR, high-throughput sequencing, and DGGE-cloning methods. The results showed that methanogenic archaea were highly predominant in both reactors but differed markedly according to community structure...
2017: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/29348732/a-flexible-system-for-cultivation-of-methanococcus-and-other-formate-utilizing-methanogens
#10
Feng Long, Liangliang Wang, Boguslaw Lupa, William B Whitman
Many hydrogenotrophic methanogens use either H2 or formate as the major electron donor to reduce CO2 for methane production. The conventional cultivation of these organisms uses H2 and CO2 as the substrate with frequent replenishment of gas during growth. H2 is explosive and requires an expensive gassing system to handle safely. Formate is as an ideal alternative substrate from the standpoints of both economy and safety but leads to large changes in the culture pH during growth. Here, we report that glycylglycine is an inexpensive and nontoxic buffer suitable for growth of Methanococcus maripaludis and Methanothermococcus okinawensis ...
2017: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/29234237/expression-purification-and-characterization-of-r-sulfolactate-dehydrogenase-comc-from-the-rumen-methanogen-methanobrevibacter-millerae-sm9
#11
Yanli Zhang, Linley R Schofield, Carrie Sang, Debjit Dey, Ron S Ronimus
( R )-Sulfolactate dehydrogenase (EC 1.1.1.337), termed ComC, is a member of an NADH/NADPH-dependent oxidoreductase family of enzymes that catalyze the interconversion of 2-hydroxyacids into their corresponding 2-oxoacids. The ComC reaction is reversible and in the biosynthetic direction causes the conversion of ( R )-sulfolactate to sulfopyruvate in the production of coenzyme M (2-mercaptoethanesulfonic acid). Coenzyme M is an essential cofactor required for the production of methane by the methyl-coenzyme M reductase complex...
2017: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/29230105/growth-characteristics-of-methanomassiliicoccus-luminyensis-and-expression-of-methyltransferase-encoding-genes
#12
Lena Kröninger, Jacqueline Gottschling, Uwe Deppenmeier
DNA sequence analysis of the human gut revealed the presence a seventh order of methanogens referred to as Methanomassiliicoccales. Methanomassiliicoccus luminyensis is the only member of this order that grows in pure culture. Here, we show that the organism has a doubling time of 1.8 d with methanol + H2 and a growth yield of 2.4 g dry weight/mol CH4 . M. luminyensis also uses methylamines + H2 (monomethylamine, dimethylamine, and trimethylamine) with doubling times of 2.1-2.3 d. Similar cell yields were obtained with equimolar concentrations of methanol and methylamines with respect to their methyl group contents...
2017: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/29225512/development-of-the-multiple-gene-knockout-system-with-one-step-pcr-in-thermoacidophilic-crenarchaeon-sulfolobus-acidocaldarius
#13
Shoji Suzuki, Norio Kurosawa
Multiple gene knockout systems developed in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius are powerful genetic tools. However, plasmid construction typically requires several steps. Alternatively, PCR tailing for high-throughput gene disruption was also developed in S. acidocaldarius , but repeated gene knockout based on PCR tailing has been limited due to lack of a genetic marker system. In this study, we demonstrated efficient homologous recombination frequency (2.8 × 104  ± 6.9 × 103 colonies/ μ g DNA) by optimizing the transformation conditions...
2017: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/28694737/archaeal-diversity-and-co-2-fixers-in-carbonate-siliciclastic-rock-groundwater-ecosystems
#14
Cassandre Sara Lazar, Wenke Stoll, Robert Lehmann, Martina Herrmann, Valérie F Schwab, Denise M Akob, Ali Nawaz, Tesfaye Wubet, François Buscot, Kai-Uwe Totsche, Kirsten Küsel
Groundwater environments provide habitats for diverse microbial communities, and although Archaea usually represent a minor fraction of communities, they are involved in key biogeochemical cycles. We analysed the archaeal diversity within a mixed carbonate-rock/siliciclastic-rock aquifer system, vertically from surface soils to subsurface groundwater including aquifer and aquitard rocks. Archaeal diversity was also characterized along a monitoring well transect that spanned surface land uses from forest/woodland to grassland and cropland...
2017: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/28634434/an-acidic-exopolysaccharide-from-haloarcula-hispanica-atcc33960-and-two-genes-responsible-for-its-synthesis
#15
Yang Lü, Hua Lu, Shiwei Wang, Jing Han, Hua Xiang, Cheng Jin
A 1.1 × 106  Da acidic exopolysaccharide (EPS) was purified from an extremely halophilic archaeon Haloarcula hispanica ATCC33960 with a production of 30 mg L-1 when grown in AS-168 medium, which mainly composed of mannose and galactose with a small amount of glucose in a molar ratio of 55.9 : 43.2 : 0.9. Two glycosyltransferase genes ( HAH_1662 and HAH_1667 ) were identified to be responsible for synthesis of the acidic EPS. Deletion of either HAH_1662 or HAH_1667 led to loss of the acidic EPS...
2017: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/28634433/the-draft-genome-of-the-non-host-associated-methanobrevibacter-arboriphilus-strain-dh1-encodes-a-large-repertoire-of-adhesin-like-proteins
#16
Anja Poehlein, Rolf Daniel, Henning Seedorf
Methanobrevibacter arboriphilus strain DH1 is an autotrophic methanogen that was isolated from the wetwood of methane-emitting trees. This species has been of considerable interest for its unusual oxygen tolerance and has been studied as a model organism for more than four decades. Strain DH1 is closely related to other host-associated Methanobrevibacter species from intestinal tracts of animals and the rumen, making this strain an interesting candidate for comparative analysis to identify factors important for colonizing intestinal environments...
2017: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/28536498/catalytic-intermediate-crystal-structures-of-cysteine-desulfurase-from-the-archaeon-thermococcus-onnurineus-na1
#17
Thien-Hoang Ho, Kim-Hung Huynh, Diem Quynh Nguyen, Hyunjae Park, Kyoungho Jung, Bookyo Sur, Yeh-Jin Ahn, Sun-Shin Cha, Lin-Woo Kang
Thermococcus onnurineus NA1 is an anaerobic archaeon usually found in a deep-sea hydrothermal vent area, which can use elemental sulfur (S0 ) as a terminal electron acceptor for energy. Sulfur, essential to many biomolecules such as sulfur-containing amino acids and cofactors including iron-sulfur cluster, is usually mobilized from cysteine by the pyridoxal 5'-phosphate- (PLP-) dependent enzyme of cysteine desulfurase (CDS). We determined the crystal structures of CDS from Thermococcus onnurineus NA1 (ToCDS), which include native internal aldimine (NAT), gem-diamine (GD) with alanine, internal aldimine structure with existing alanine (IAA), and internal aldimine with persulfide-bound Cys356 (PSF) structures...
2017: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/28348508/construction-of-expression-shuttle-vectors-for-the-haloarchaeon-natrinema-sp-j7-based-on-its-chromosomal-origins-of-replication
#18
Yuchen Wang, Beibei Chen, Linshan Sima, Mengzhuo Cao, Xiangdong Chen
Haloarchaeon Natrinema sp. J7, the first reported archaeon harboring both plasmid and chromosome-based temperate viruses, is a useful model for investigating archaeal virus-host and virus-virus interactions. However, the lack of genetic tools has limited such studies. On the basis of the automatically replicating sequences of the J7 chromosome and the pyrF marker, we constructed seven vectors, six of which were confirmed to possess replication ability in a pyrF- deletion derivative of J7 (J7-F). Among these vectors, pFJ1, pFJ4, and pFJ6 could be transformed into the host strain with relatively high efficiency (approximately 103 colony-forming units/ μ g DNA) and were present at about one copy per chromosome...
2017: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/28239294/transfection-studies-with-colloidal-systems-containing-highly-purified-bipolar-tetraether-lipids-from-sulfolobus-acidocaldarius
#19
Konrad H Engelhardt, Shashank Reddy Pinnapireddy, Elias Baghdan, Jarmila Jedelská, Udo Bakowsky
Lipid vectors are commonly used to facilitate the transfer of nucleic acids into mammalian cells. In this study, two fractions of tetraether lipids from the archaea Sulfolobus acidocaldarius were extracted and purified using different methods. The purified lipid fractions polar lipid fraction E (PLFE) and hydrolysed glycerol-dialkyl-nonitol tetraether (hGDNT) differ in their structures, charge, size, and miscibility from conventional lipids. Liposomes were prepared by mixing tetraether lipids with cholesterol (CH) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) resulting in stable vectors for gene delivery...
2017: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/28154498/reverse-methanogenesis-and-respiration-in-methanotrophic-archaea
#20
REVIEW
Peer H A Timmers, Cornelia U Welte, Jasper J Koehorst, Caroline M Plugge, Mike S M Jetten, Alfons J M Stams
Anaerobic oxidation of methane (AOM) is catalyzed by anaerobic methane-oxidizing archaea (ANME) via a reverse and modified methanogenesis pathway. Methanogens can also reverse the methanogenesis pathway to oxidize methane, but only during net methane production (i.e., "trace methane oxidation"). In turn, ANME can produce methane, but only during net methane oxidation (i.e., enzymatic back flux). Net AOM is exergonic when coupled to an external electron acceptor such as sulfate (ANME-1, ANME-2abc, and ANME-3), nitrate (ANME-2d), or metal (oxides)...
2017: Archaea: An International Microbiological Journal
journal
journal
40162
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"