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Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia

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https://www.readbyqxmd.com/read/30238936/changes-in-cholesterol-metabolism-during-acute-upper-gastrointestinal-bleeding-liver-cirrhosis-and-non-cirrhosis-compared
#1
Vladimir Hrabovsky, Vladimir Blaha, Radomir Hyspler, Alena Ticha, Martina Skrobankova, Zdenek Svagera
BACKGROUND: Cholesterol is derived via de novo synthesis and dietary absorption. Both processes can be monitored by determination of non-cholesterol sterol concentrations (lathosterol for synthesis; sitosterol and campesterol for absorption). The hypocholesterolemia that occurs during acute illness is a result of a multifactorial inability to compensate for the increased needs for this metabolite. The aim of this study was to examine the plasma cholesterol profile and both processes of cholesterol acquisition during acute upper gastrointestinal haemorrhage with emphasis on liver cirrhosis...
September 20, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30238935/gestational-surrogacy-in-the-czech-republic
#2
David Rumpik, Tatana Rumpikova, Michal Pohanka, Pavel Ventruba, Silvie Belaskova
BACKGROUND: Gestational surrogacy, is a treatment option for women with certain clearly defined medical problems, usually an absent uterus, to help them have their own genetic children. The aim of our study was to review, evaluate and share our experience and outcomes over the last 13 years of the largest surrogacy program in the Czech republic. METHODS: A total of 75 intended mothers and 82 surrogate mothers participated in this study. A retrospective cohort study was performed...
September 20, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30238934/descemet-membrane-endothelial-keratoplasty-with-stromal-rim-dmek-s-in-complicated-patients
#3
Pavel Studeny, Miroslav Veith, Deli Krizova
PURPOSE: To evaluate the outcomes of the hybrid technique of posterior lamellar keratoplasty (Descemets' membrane endothelial keratoplasty with stromal rim, DMEK-S) in eyes with multiple ocular pathologies. METHODS: A retrospective case control study of the consecutive group of all eyes with combined ocular pathologies which underwent DMEK-S keratoplasty. We evaluated the number of pre- and post-operative complications; uncorrected distance visual acuity (UDVA); corrected distance visual acuity (CDVA) and endothelial cell density (ECD)...
September 20, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30215436/comparison-of-different-methods-of-abi-acquisition-for-detection-of-peripheral-artery-disease-in-diabetic-patients
#4
Miroslav Homza, Ondrej Machaczka, Martin Porzer, Milan Kozak, Jiri Plasek, David Sipula
BACKGROUND: Ankle brachial index (ABI) is the principal screening method for peripheral arterial disease (PAD). In this study, we compare various types of Doppler-derived and oscillometric ABIs with results obtained through duplex ultrasonography. METHODS: 62 patients were enrolled in the study. For each limb, blood pressures for both ankle arteries and the arm were measured using Doppler and an automated oscillometric device. Duplex ultrasound was performed for all limbs and occlusions >50% were considered PAD-positive...
September 14, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30215435/from-open-packing-to-negative-wound-pressure-therapy-a-critical-overview-of-deep-sternal-wound-infection-treatment-strategies-after-cardiac-surgery
#5
Martin Simek, Adam Chudoba, Roman Hajek, Patrick Tobbia, Martin Molitor, Petr Nemec
Deep sternal wound infection is a challenging aspect of modern cardiac surgery. The considerable mortality rate, devastating morbidity and, negative impact on long-term survival has driven cardiac and plastic surgeons to seek a more advantageous treatment solution. This review summarizes progress in the field of deep sternal wound infection treatment after cardiac surgery. Emphasis is placed on outcomes analysis of contemporary treatment strategy based on negative pressure wound therapy followed by sternotomy wound reconstruction, and its comparison with conventional treatment modalities used afore...
September 14, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30214077/midterm-outcomes-of-mini-invasive-surgical-and-hybrid-ablation-of-atrial-fibrillation
#6
Marek Pojar, Ludek Haman, Petr Parizek, Andrej Myjavec, Jan Gofus, Jan Vojacek
BACKGROUND: We evaluated the feasibility and effectiveness of thoracoscopic and a staged surgical and transcatheter ablation technique to treat stand-alone atrial fibrillation (AF). METHODS: . Between 2009 and 2016, a cohort of 65 patients underwent bilateral totally thoracoscopic ablation of symptomatic paroxysmal AF (n=30; 46%), persistent AF (n=18; 28%) or long-standing persistent AF (n=17; 26%) followed by catheter ablation in case of AF recurrence. Surgical box lesion procedure included bilateral pulmonary vein and left atrial posterior wall ablation using irrigated bipolar radiofrequency with documentation of conduction block...
September 13, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30209439/normotensive-glaucoma
#7
Jan Lestak, Nada Jiraskova, Marketa Zakova, Marketa Stredova
This article provides an updated overview of the structural and functional changes in normotensive glaucoma and its variations from hypertensive glaucoma. The authors point out the less familiar facts in which both diagnostic groups differ.
September 11, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30209438/association-of-serum-adipocyte-fatty-acid-binding-protein-and-apolipoprotein-b-apolipoprotein-a1-ratio-with-intima-media-thickness-of-common-carotid-artery-in-dyslipidemic-patients
#8
Jiri Orsag, David Karasek, Milan Halenka, Helena Vaverkova, Jaromira Spurna, Veronika Kubickova, Jiri Lukes, Josef Zadrazil
BACKGROUND: Diseases caused by atherosclerosis play the most important role in mortality and morbidity worldwide. Serum adipocyte fatty acid binding protein (A-FABP) seems to be a new promising marker to determine the risk of atherosclerosis. OBJECTIVE: The aim of this study was to evaluate relationships between serum A-FABP levels in studied individuals and to assess the possibility of modeling the intima media thickness of the common carotid artery (C-IMT) using A-FABP levels and other observed characteristics...
September 11, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30209437/intestinal-dysbiosis-and-hormonal-neuroendocrine-secretion-in-the-fibromyalgic-patient-relationship-and-correlations
#9
Giovanni Tomasello, Margherita Mazzola, Vincenzo Bosco, Giulia Tomasello, Provvidenza Damiani, Emanuele Sinagra, Francesco Carini
Fibromyalgia is a rheumatic syndrome and its pathogenesis is controversial. The recent literature has placed considerable attention on the link between alteration of the intestinal microbiota and fibromyalgia, emphasizing the close connection between the neuroenteric system and the CNS. This study aims to evaluate the probable relationship between intestinal dysbiosis and altered secretion of hormones and vitamins such as cortisol, serotonin, Vitamin D and thyroid hormones in a patient with fibromyalgia.
September 11, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30209435/apparent-foreign-body-like-structure-detected-in-the-left-atrium-after-catheter-ablation-for-persistent-atrial-fibrillation
#10
Tomas Skala, Ondrej Moravec, Martin Hutyra, Zbynek Tudos, Martin Kocher, Milos Taborsky
BACKGROUND: A foreign body left in left atrium after catheter ablation of atrial fibrillation is a very rare complication. Nevertheless, there are no reports so far about a large newly emerged structure only giving the impression of being a foreign body left in the heart after catheter ablation. CASE REPORT: This report presents a case of a patient after catheter ablation for persistent atrial fibrillation. Because of atrial tachycardia during follow-up, patient was indicated for reablation...
September 11, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30209436/abdominal-wall-allotransplantation
#11
Martin Molitor, Martin Oliverius, Andrej Sukop
Allotransplantation of vascularized composite tissue is a new field of transplantation surgery. One application of this technique is abdominal wall transplantation used as a supplementary procedure to the transplantation of visceral organs in patients with abdominal compartment deficits. As abdominal wall closure problems are experienced in around 30-40% of such patients, peer reviewed conclusions on the viability of various options, are important for an informed choice of possible procedures. This review focuses on the abdominal wall allotransplantion procedure...
September 7, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30198521/ciprofloxacin-prophylaxis-during-autologous-stem-cell-transplantation-for-multiple-myeloma-in-patients-with-a-high-rate-of-fluoroquinolone-resistant-gram-negative-bacteria-colonization
#12
Alzbeta Zavrelova, Pavla Paterova, Filip Gabalec, Pavel Zak, Jakub Radocha
BACKGROUND: Ciprofloxacin prophylaxis used to be a standard precaution during autologous stem cell transplantation. Its benefit, with a high prevalence of fluoroqinolone resistance in the population, has recently been under scrutiny. OBJECTIVE: To evaluate the impact of cessation of ciprofloxacin prophylaxis during stem cell transplantation for multiple myeloma. PATIENTS AND METHODS: Data from 104 patients with multiple myeloma transplanted during the period from January 2013 to April 2015 were retrospectively reviewed...
September 7, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30198520/therapeutic-plasma-exchange-in-secondary-prevention-of-acute-pancreatitis-in-pregnant-patient-with-familial-hyperchylomicronemia
#13
Renata Michalova, Anna Mankova, Matej Vnucak, Stanislava Mikulova, Frantisek Nehaj, Katarina Raslova, Ivana Dedinska, Alena Jezikova, Pavel Makovicky, Peter Galajda, Marian Mokan
INTRODUCTION: Hormone changes during pregnancy lead to increased plasma lipid levels. When there is added disorder of lipid metabolism, this otherwise physiological change can cause extremely high triglyceride levels with potentionally life-threatening complications, such as non-biliary acute pancreatitis. MATERIALS AND METHODS: We present a case report of a 27-year-old pregnant woman with familial hyperchylomicronemia and a history of 7 hypertriglyceridemia-induced acute pancreatitis attacks...
September 7, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30198519/serum-c-peptide-level-correlates-with-the-course-of-muscle-tissue-healing-in-the-rabbit-model-of-critical-limb-ischemia
#14
Zdenek Tauber, Katerina Cizkova, Maria Janikova, Jana Jurcikova, Katerina Vitkova, Lubomir Pavliska, Ludmila Porubova, Agata Krauze, Carlos Fernandez, Frantisek Jaluvka, Iveta Spackova, Ivo Lochman, Martin Prochazka, Brian H Johnstone, Vaclav Prochazka
AIM: The therapeutic potential of adipose-derived stem cell conditioned medium (ASC-CM) was studied in the rabbit model of critical limb ischemia (CLI). METHODS: Rabbits received treatment with ASC-CM or placebo. Gastrocnemius muscle tissue was collected 35 days after ischemia induction. Ischemic changes were evaluated in hematoxylin-eosin stained tissues for early (necrotic lesions/granulation tissue) and late (fibrous scars) phases of tissue repair. The expression of proangiogenic miR-126 was also evaluated using in situ hybridization...
September 7, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30198518/associations-between-congenital-heart-defects-and-genetic-and-morphological-anomalies-the-importance-of-prenatal-screening
#15
Jan Pavlicek, Tomas Gruszka, Sabina Kapralova, Martin Prochazka, Eva Silhanova, Romana Kaniova, Slavka Polanska, Renata Cernickova, Eva Klaskova
AIM: To study congenital heart defects (CHDs), evaluate their relation to extra-cardiac pathologies, and assess the significance of prenatal diagnostics for heart diseases. METHODS: Data from 1999-2017 were analyzed for the incidence of significant CHDs in fetuses (prenatal ultrasound/echocardiography) and children, including, where applicable, autopsy data and genetic evaluation. RESULTS: Among 220,400 fetuses, 819 (3.7 cases per 1000) significant CHDs were observed...
September 6, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30181666/safety-of-early-discharge-in-low-risk-patients-after-acute-st-segment-elevation-myocardial-infarction-treated-with-primary-percutaneous-coronary-intervention-open-label-randomized-trial
#16
Kamil Novobilskyª, Roman Stipalª, Petr Cernyª, Ivo Horakª, Vladimir Kaucakª, Jan Mrozekª, Jan Vaclavik, Radim Kryzaª
BACKGROUND: The length of hospital stay in patients with acute myocardial infarction and ST-segment elevation (STEMI) has been shortened in recent years with corresponding savings in costs, but there is limited available data on its implementation in clinical practice. The aim of this trial was to determine whether early discharge in selected patients after STEMI is feasible and safe. METHODS: 151 patients with STEMI successfully treated with primary percutaneous coronary intervention (PCI) who fulfilled the inclusion criteria of low risk were randomly assigned to two groups on a 1:1 ratio: early (within 48-72 h of admission) and standard (after 72 h) discharge...
August 28, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30181665/ecg-non-gated-multi-detector-computed-tomography-protocol-prior-to-catheter-ablation-of-atrial-fibrillation-provides-sufficient-data-quality-with-lower-radiation-exposure-compared-to-ecg-gated-protocol-results-of-a-prospective-randomized-and-blinded-study
#17
Zbynek Tudos, Tomas Skala, Martin Homola, Ondrej Moravec, Milos Taborsky, Martin Kocher, Marie Cerna, Filip Ctvrtlik, Frantisek Odstrcil, Katerina Langova, Olga Klementova
BACKGROUND: The role of ECG-gating in left atrium (LA) computed tomography (MDCT) imaging is not precisely defined. METHODS AND RESULTS: 62 patients were randomized according to ECG gating with prospective evaluation of image quality, Volume CT Dose Index, Dose Length Product, Effective Dose and registration error between anatomical map and MDCT. We found significant difference in all radiation variables, but not in visual quality, registration error, CA duration, CA fluoroscopy time and CA fluoroscopy dose...
August 28, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30181664/the-effects-of-o-glcnac-alteration-on-alzheimer-like-neurodegeneration-in-sk-n-sh-cells
#18
Nan Ding, Peng Peng, Yu-Jing Chu, Jing-Jing Wang, Shu-Yi Chen, Renuka Arulthas, Yan-Qiu Deng
BACKGROUND: O-GlcNAcylation is a highly dynamic post-translational modification that plays a key role in regulating phosphorylation of protein and cell survival. The proteins O-GlcNAcylation level is regulated dynamically by O-GlcNAc transferase (OGT) and β-N-acetylglucosaminidase (O-GlcNAcase, OGA). Although previous studies have suggested the role of O-GlcNAcylation in neurodegenerative diseases, the mechanism of O-GlcNAcylation in Alzheimer's disease (AD) remains unclear. METHODS: The decrease of O-GlcNAcylation by alloxan, an OGT inhibitor, and increase by NAG-thiazolines (NAG-Ae), an O-GlcNAcase inhibitor were tested to investigate the effects of O-GlcNAc alteration on AD like neurodegeneration in SK-N-SH cells...
August 28, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30150791/identification-of-novel-oxa-134-like-%C3%AE-lactamases-in-acinetobacter-lwoffii-and-acinetobacter-schindleri-isolated-from-chicken-litter
#19
Patrik Mlynarcik, Jan Bardon, Miroslava Htoutou Sedlakova, Petra Prochazkova, Milan Kolar
BACKGROUND: Various food-producing animals have been recognized in recent years as a potential reservoir for the spread of antibiotic resistant bacteria that may pose a risk to human health and therefore their dissemination in the food production chain needs to be assessed. METHODS: In this study, 450 boot swabs from chicken farms were analyzed for the presence of antimicrobial resistance with a focus on β-lactams resistance in Acinetobacter species. RESULTS: Two β-lactamase-encoding genes were first time identified in Acinetobacter lwoffii and Acinetobacter schindleri isolates...
July 18, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/30150790/increased-glutamate-and-deep-brain-atrophy-can-predict-the-severity-of-multiple-sclerosis
#20
Hubert Polacek, Ema Kantorova, Petra Hnilicova, Marian Grendar, Kamil Zelenak, Egon Kurca
OBJECTIVE: In multiple sclerosis (MS), deep grey matter (DGM) atrophy has been recognised as a crucial component of the disease that presents early and it has been associated with disability. Although the precise mechanism underlying grey matter atrophy is unknown, several hypotheses have been postulated. Our previous research pointed to correlations of hypothalamic metabolic alterations with clinical outcomes of MS, therefore we decided to further test the relationship of these alterations with DGM atrophy...
July 16, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
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