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Comptes Rendus Biologies

Raëd Farhat, Ayman El-Seedy, Ariestya Indah Permata Sari, Caroline Norez, Marie-Claude Pasquet, Frédéric Becq, Alain Kitzis, Véronique Ladevèze
Cystic fibrosis is caused by mutations on the Cystic Fibrosis Transmembrane conductance Regulator gene (CFTR). Exonic mutations may have variable effect on the CFTR protein and may alter the normal localization of CFTR on the apical membrane of epithelial cells or/and its function as a chloride channel. Identifying the effect of a missense mutation can be a first step in helping the medical counseling and the therapeutic strategies. In this study, the effect of the c.965T>C exon 8 mutation that induces a valine-to-alanine substitution (p...
August 4, 2017: Comptes Rendus Biologies
Luigi Rosati, Piero Andreuccetti, Marina Prisco
VIP and its receptors (VPACRs) are largely investigated in vertebrate testis, as well as their functions in the control of spermatogenesis and steroidogenesis. By contrast, a few data are available about the presence and role of VIP in the epididymis. The aim of the present paper was to investigate the localization of VIP and its receptors in the epididymis of two vertebrates: Podarcis sicula, a seasonal reproducer, and Rattus rattus, a continuous reproducer. By immunohystochemical investigation, we demonstrated for the first time that VIP and its receptors are widely represented in the epididymis of Podarcis sand Rattus; in particular in Podarcis, we showed that during the reproductive period, as well as in Rattus, VIP and its receptors are well represented in all the epithelial cells and the connective tissue of the epididymis; by contrast, during the non-reproductive period, VIP and its receptors are represented only in the connective tissue...
August 4, 2017: Comptes Rendus Biologies
Marcelly Miranda Aybal Jayme, Rebeca Oliveira Castro, Carlos André Mandarino Silva, Mariana Muniz Silva, Flávia Lima do Carmo, Fábio Vieira de Araujo
Production of antibiotics and enzymes by microorganisms is well recognized for its economic benefits. Searching potentially exploitable microorganisms, strains of heterotrophic bacteria were isolated from marine and estuarine waters in Niterói-RJ, Brazil, and tested for the production of enzymes and antimicrobial substances. From the 76 isolated colonies, 09.21% showed antimicrobial potential, 63.16% showed enzymatic activity for at least one of the substrates tested and 91% showed multidrug resistance. Strains that presented the better results were identified by sequencing...
June 2017: Comptes Rendus Biologies
Xiangbo Xin, Ting Wang, Xinfeng Liu, Guoning Sui, Congfei Jin, Yingwei Yue, Shuping Yang, Hong Guo
CMYA1 is a protein that plays an important role in muscle development and contains a highly conserved Xin repeats region. However, the function of the Xin-repeats in CMYA1 is unknown, and there is little information regarding proteins that interact with the CMYA1/Xin-repeats in the bovine system. In this study, we generated a high-quality bovine muscle cDNA library and performed a yeast two-hybrid screen twice using both CMYA1 and the Xin-repeats as bait. There were 27 candidate-interacting proteins identified in this screen...
June 2017: Comptes Rendus Biologies
Konrad Celiński, Hanna Kijak, Aleksandra Wojnicka-Półtorak, Katarzyna Buczkowska-Chmielewska, Joanna Sokołowska, Ewa Chudzińska
DNA barcoding is a standard and efficient method, frequently used for identification, discrimination and discovery of new species. Although this approach is very useful for classifying the world's biodiversity, little is known about its usefulness in barcoding at lower taxonomic level and its discrimination rate for closely related species, like conifers. In this study, we compared the genetic variation of eight chloroplast DNA barcode regions (matK, rbcL, trnH-psbA, trnL-trnF, rpl20-rps18, trnV, ycf1, ycf2) in 17 conifers - three closely related pines from Pinus mugo complex and 14 more distant conifers representing two genera and four sections of the Pinaceae family...
June 2017: Comptes Rendus Biologies
Maria Shafique, Aqsa Jawaid, Yasir Rehman
Remediation of toxic metal-polluted sites by microorganisms is an environment-friendly remediation technique. Multi-metal-resistant bacteria were isolated from a wastewater treatment plant showing resistance against As(III), As(V), Cr, Co, Cu, Cd, Hg, Ni, Pb, Se and Zn. Maximum resistance against all metals was shown by the bacterial isolate MX-6 (As 20mM, Cd 30mM, Cr 5.0mM, Co 25mM, Cu 25mM, Ni 20mM, Zn 30mM, Pb 15mM, Se 20mM and Hg 2.5mM), which was identified as Pseudomonas sp. through 16S rDNA sequencing...
June 2017: Comptes Rendus Biologies
Wilson R Lourenço
Plethoscorpiops profusus gen. n., sp. n., belonging to the family Scorpiopidae Kraepelin, 1905 is described on the basis of two specimens, one adult female and one male juvenile collected in the Saddan Cave, in Kayin State, Hpa-An, Burma (Myanmar). This new scorpion taxon most certainly represents an endemic element for the fauna of Burma and seems to be strictly distributed inside the cave system. The new genus is characterized by a previously unknown and totally unique plethotaxic trichobothrial pattern within the family Scorpiopidae...
June 2017: Comptes Rendus Biologies
Babak Abdollahi Mandoulakani, Shilan Nasri, Sahar Dashchi, Sorour Arzhang, Iraj Bernousi, Hossein Abbasi Holasou
The identification of polymorphic markers associated with various quantitative traits allows us to test their performance for the exploitation of the extensive quantitative variation maintained in gene banks. In the current study, a set of 97 wheat germplasm accessions including 48 cultivars and 49 breeding lines were evaluated for 18 agronomic traits. The accessions were also genotyped with 23 ISSR, nine IRAP and 20 REMAP markers, generating a total of 658 clear and scorable bands, 86% of which were polymorphic...
June 2017: Comptes Rendus Biologies
Nicolas Bacaër
This study focuses on the speed of extinction of a population living in a random environment that follows a continuous-time Markov chain. Each individual dies or reproduces at a rate that depends on the environment. The number of offspring during reproduction follows a given probability law that also depends on the environment. In the so-called subcritical case where the population goes for sure to extinction, there is an explicit formula for the speed of extinction. In some sense, environmental stochasticity slows down population extinction...
May 2017: Comptes Rendus Biologies
Fatima Z Maghnia, Hervé Sanguin, Younes Abbas, Marcello Verdinelli, Benaissa Kerdouh, Naima El Ghachtouli, Enrico Lancellotti, Salah Eddine Bakkali Yakhlef, Robin Duponnois
The cork oak forest is an ecosystem playing a major role in Moroccan socio-economy and biodiversity conservation. However, this ecosystem is negatively impacted by extensive human- and climate-driven pressures, causing a strong decrease in its distribution and a worsening of the desertification processes. This study aims at characterising the impact of cork oak forest management on a major actor of its functioning, the ectomycorrhizal (EcM) fungal community associated with Quercus suber, and the determination of EcM bio-indicators...
May 2017: Comptes Rendus Biologies
Mohamed Toumi, Selma Barris, Mohamed Seghiri, Houmam Cheriguene, Fatiha Aid
The planting of several species adapted to forests areas in the Saharan Atlas would allow one to better fight against the desertification of the vegetation cover of these fragile areas. Thanks to its rapid growth on degraded soils, Robinia pseudoacacia L. has an advantage in the repopulation of these areas undergoing desertification. Operation of this large-scale tree requires good control of germination conditions and growth of plants. The scarification of the seeds of Robinia pseudoacacia L. is necessary to allow the absorption of water by the seeds...
May 2017: Comptes Rendus Biologies
Alastair A Macdonald
Patterns of wear on the canine teeth were examined in 260 adult male babirusa skulls. Video recordings of 161 adult male babirusas at salt licks in North Sulawesi were viewed for behaviour related to erosion of the canine teeth. Corresponding observations were made of the behaviour of male babirusa in Antwerp and Surabaya Zoos. Most loss of maxillary canine dental tissue was from the ventro-lateral or lateral surface of the tooth, towards its distal end. The teeth were smoothly flattened on a plane approximating to the plane formed on the side of the face by the snout, the zygion and the zygomatic process...
May 2017: Comptes Rendus Biologies
Carlos E Santibáñez-López, Edmundo González-Santillán
Diplocentrus duende n. sp. is described based on adult males collected from a locality in the Tehuacán-Cuicatlán Valley, Mexico. This species has punctate pedipalp surfaces, a condition present only in four other species of this specious genus. As suggested here, this condition has evolved independently in these species within the "mexicanus" group of Diplocentrus from the rest of the diplocentrids.
May 2017: Comptes Rendus Biologies
Ibtissem Laaribi, Hassouna Gouta, Mouna Mezghani Ayachi, Foued Labidi, Messaoud Mars
Increasing olive germplasm erosion in the coastline of Tunisia has required an imperious conservation of the traditional genotypes before an ultimate disappearance. This region has been relatively neglected in the literature sources of olive identification. In this context, a prospection effort and a preliminary selection of olive accessions belonging to Central-Eastern Tunisia was carried out. Twenty-seven ancient olive accessions were studied by combining molecular and morphological data in order to fingerprint them, and to evaluate their relationships with classical cultivars...
May 2017: Comptes Rendus Biologies
Ramasamy Naveenkumar, Arjunan Muthukumar, Ganesan Sangeetha, Ramanathan Mohanapriya
Three plant oils (Cymbopogon citratus, Cymbopogon martini, and Pelargonium graveolens) were developed as EC formulations and tested for their physical stabilities. EC formulations (10EC, 20EC and 30EC) of C. citratus, C. martini and P. graveolens had emulsion stability, spontaneity property, heat and cold stability. EC formulated plant oils were screened against the major seed borne fungi of rice such as Curvularia lunata, Fusarium moniliforme, Bipolaris oryzae, and Sarocladium oryzae. The level of inhibition varied among the concentrations of EC formulations...
April 2017: Comptes Rendus Biologies
Rosaria Scudiero, Maria Grazia Esposito, Palma Simoniello, Chiara Maria Motta
Nonessential metal cadmium is widely used and released in the environment, causing cell toxicity and posing a severe threat to wildlife. Zebrafish (Danio rerio) is one of the most commonly used animals in the investigation of environmental cadmium toxicity in vertebrates. In this study, we identified two cadmium-responsive genes, RBBP6 and CRYL1, in the early phases of zebrafish development, at the gastrula stage. The retinoblastoma binding protein 6 is associated with increased protein degradation and cell proliferation; crystallin-lambda 1 is a lens protein with redox activity...
April 2017: Comptes Rendus Biologies
Xiaolong Wang, Lin Li, Jiaxin Zhao, Fangliang Li, Wei Guo, Xia Chen
To evaluate the effects of different preservation methods (stored in a -20°C ice chest, preserved in liquid nitrogen and dried in silica gel) on inter simple sequence repeat (ISSR) or random amplified polymorphic DNA (RAPD) analyses in various botanical specimens (including broad-leaved plants, needle-leaved plants and succulent plants) for different times (three weeks and three years), we used a statistical analysis based on the number of bands, genetic index and cluster analysis. The results demonstrate that methods used to preserve samples can provide sufficient amounts of genomic DNA for ISSR and RAPD analyses; however, the effect of different preservation methods on these analyses vary significantly, and the preservation time has little effect on these analyses...
April 2017: Comptes Rendus Biologies
Florence Lormières, Phil M Oger
The bacterial diversity of a naturally seeping bitumen source was investigated by 16S rRNA gene cloning and sequencing. Epsilonproteobacteria were shown to dominate the bacterial diversity in the underground water and within the bitumen, representing ca. 75% of the total bacterial diversity. These Epsilonproteobacteria were dominated by Sulfurimonas OTUs, while Sulfurovum and Arcobacter OTUs completed the remaining diversity. Epsilonproteobacteria are sulfur-oxidizer, nitrate-reducing chemo-lithoautotrophic bacteria, unable to use most organics for growth but capable of CO2 fixation...
April 2017: Comptes Rendus Biologies
Tamara G Petrović, Tanja D Vukov, Nataša Tomašević Kolarov
Current studies of anuran morphology use sacral vertebrae, hindlimbs as main predictor for locomotor modes, while forelimbs and their segments were not analysed in this context. Due the fact that the complex interplay of numerous morphological traits determines the locomotion ability of anurans, we used ratios for both fore- and hindlimbs to obtain useful information of the structural and functional properties of locomotor system. We examined patterns of variation in total length of fore-, and hindlimbs as well as sacral vertebrae width and diapophyseal expansion in order to position two locomotor modes (jumpers and walkers) in a morphological shape space...
April 2017: Comptes Rendus Biologies
Kinjal Samir Patel, Jinal Hardik Naik, Sejal Chaudhari, Natarajan Amaresan
To elucidate the functional diversity of hot spring bacteria, 123 bacteria were isolated and screened for evaluating their multifunctional plant growth promoting (PGP) properties. The antagonistic activity against different phytopathogens showed the presence of a high amount of biocontrol bacteria in the hot springs. During screening for PGP properties, 61.0% isolates showed production of indole acetic acid and 23.6% showed inorganic phosphate solubilization qualitatively. For production of extracellular enzymes, it was found that 61...
April 2017: Comptes Rendus Biologies
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