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Microbial Cell Factories

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https://www.readbyqxmd.com/read/28431563/inheritance-of-brewing-relevant-phenotypes-in-constructed-saccharomyces-cerevisiae%C3%A2-%C3%A3-%C3%A2-saccharomyces-eubayanus-hybrids
#1
Kristoffer Krogerus, Tuulikki Seppänen-Laakso, Sandra Castillo, Brian Gibson
BACKGROUND: Interspecific hybridization has proven to be a potentially valuable technique for generating de novo lager yeast strains that possess diverse and improved traits compared to their parent strains. To further enhance the value of hybridization for strain development, it would be desirable to combine phenotypic traits from more than two parent strains, as well as remove unwanted traits from hybrids. One such trait, that has limited the industrial use of de novo lager yeast hybrids, is their inherent tendency to produce phenolic off-flavours; an undesirable trait inherited from the Saccharomyces eubayanus parent...
April 21, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28431560/a-new-generation-of-bacillus-subtilis-cell-factory-for-further-elevated-scyllo-inositol-production
#2
Kosei Tanaka, Ayane Natsume, Shu Ishikawa, Shinji Takenaka, Ken-Ichi Yoshida
BACKGROUND: A stereoisomer of inositol, scyllo-inositol (SI), has been regarded as a promising therapeutic agent for Alzheimer's disease. However, this compound is relatively rare, whereas another stereoisomer of inositol, myo-inositol (MI) is abundant in nature. Bacillus subtilis 168 has the ability to metabolize inositol stereoisomers, including MI and SI. Previously, we reported a B. subtilis cell factory with modified inositol metabolism that converts MI into SI in the culture medium...
April 21, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28424096/metabolic-and-proteomic-analyses-of-product-selectivity-and-redox-regulation-in-clostridium-pasteurianum-grown-on-glycerol-under-varied-iron-availability
#3
Christin Groeger, Wei Wang, Wael Sabra, Tyll Utesch, An-Ping Zeng
BACKGROUND: Clostridium pasteurianum as an emerging new microbial cell factory can produce both n-butanol (BuOH) and 1,3-propanediol (1,3-PDO), and the pattern of product formation changes significantly with the composition of the culture medium. Among others iron content in the medium was shown to strongly affect the products selectivity. However, the mechanism behind this metabolic regulation is still unclear. For a better understanding of such metabolic regulation and for process optimization, we carried out fermentation experiments under either iron excess or iron limitation conditions, and performed metabolic, stoichiometric and proteomic analyses...
April 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28424079/erratum-to-rhodococcus-erythropolis-as-a-host-for-expression-secretion-and-glycosylation-of-mycobacterium-tuberculosis-proteins
#4
Antonio J Vallecillo, Cristina Parada, Pedro Morales, Clara Espitia
No abstract text is available yet for this article.
April 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28420406/genomics-insights-into-different-cellobiose-hydrolysis-activities-in-two-trichoderma-hamatum-strains
#5
Peng Cheng, Bo Liu, Yi Su, Yao Hu, Yahui Hong, Xinxin Yi, Lei Chen, Shengying Su, Jeffrey S C Chu, Nansheng Chen, Xingyao Xiong
BACKGROUND: Efficient biomass bioconversion is a promising solution to alternative energy resources and environmental issues associated with lignocellulosic wastes. The Trichoderma species of cellulolytic fungi have strong cellulose-degrading capability, and their cellulase systems have been extensively studied. Currently, a major limitation of Trichoderma strains is their low production of β-glucosidases. RESULTS: We isolated two Trichoderma hamatum strains YYH13 and YYH16 with drastically different cellulose degrading efficiencies...
April 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28410609/engineered-microbial-biosensors-based-on-bacterial-two-component-systems-as-synthetic-biotechnology-platforms-in-bioremediation-and-biorefinery
#6
REVIEW
Sambandam Ravikumar, Mary Grace Baylon, Si Jae Park, Jong-Il Choi
Two-component regulatory systems (TCRSs) mediate cellular response by coupling sensing and regulatory mechanisms. TCRSs are comprised of a histidine kinase (HK), which serves as a sensor, and a response regulator, which regulates expression of the effector gene after being phosphorylated by HK. Using these attributes, bacterial TCRSs can be engineered to design microbial systems for different applications. This review focuses on the current advances in TCRS-based biosensors and on the design of microbial systems for bioremediation and their potential application in biorefinery...
April 14, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28403896/gas-fermentation-cellular-engineering-possibilities-and-scale-up
#7
REVIEW
Björn D Heijstra, Ching Leang, Alex Juminaga
Low carbon fuels and chemicals can be sourced from renewable materials such as biomass or from industrial and municipal waste streams. Gasification of these materials allows all of the carbon to become available for product generation, a clear advantage over partial biomass conversion into fermentable sugars. Gasification results into a synthesis stream (syngas) containing carbon monoxide (CO), carbon dioxide (CO2), hydrogen (H2) and nitrogen (N2). Autotrophy-the ability to fix carbon such as CO2 is present in all domains of life but photosynthesis alone is not keeping up with anthropogenic CO2 output...
April 12, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28403867/accelerated-triacylglycerol-production-and-altered-fatty-acid-composition-in-oleaginous-microalga-neochloris-oleoabundans-by-overexpression-of-diacylglycerol-acyltransferase-2
#8
Paeka Klaitong, Sirirat Fa-Aroonsawat, Wipa Chungjatupornchai
BACKGROUND: Microalgae are promising sources of lipid triacylglycerol (TAG) for biodiesel production. However, to date, microalgal biodiesel is technically feasible, but not yet economically viable. Increasing TAG content and productivity are important to achieve economic viability of microalgal biodiesel. To increase TAG content, oleaginous microalga Neochloris oleoabundans was genetically engineered with an endogenous key enzyme diacylglycerol acyltransferase 2 (NeoDGAT2) responsible for TAG biosynthesis...
April 12, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28388915/improved-curdlan-production-with-discarded-bottom-parts-of-asparagus-spear
#9
Rex Frimpong Anane, Huifang Sun, Lamei Zhao, Le Wang, Chun Lin, Zichao Mao
BACKGROUND: This work evaluated the improvement of curdlan production of Agrobacterium sp. ATCC 31749 by using culture medium containing juice of discarded bottom part of green Asparagus spear (MJDA). Curdlan production was carried out using Agrobacterium sp. ATCC 31749 in flasks with different volumes of MJDA and its non-juice-adding control (CK) incubated in shaker at 30 °C, 200 rpm rotation for 168 h. RESULTS: All MJDA media increased Agrobacterium sp. ATCC 31749 cell mass and enhanced the cells' ability to utilise sucrose, the carbon source for curdlan biosynthesis, and thereby produced higher concentration of curdlan than CK which is used for commercial production of curdlan...
April 7, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28381263/engineering-halomonas-species-td01-for-enhanced-polyhydroxyalkanoates-synthesis-via-crispri
#10
Wei Tao, Li Lv, Guo-Qiang Chen
BACKGROUND: Clustered regularly interspaced short palindromic repeats interference (CRISPRi) has provided an efficient approach for targeted gene inhibition. A non-model microorganism Halomonas species TD01 has been developed as a promising industrial producer of polyhydroxyalkanoates (PHA), a family of biodegradable polyesters accumulated by bacteria as a carbon and energy reserve compound. A controllable gene repression system, such as CRISPRi, is needed for Halomonas sp. TD01 to regulate its gene expression levels...
April 6, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28381218/development-of-an-efficient-technique-for-gene-deletion-and-allelic-exchange-in-geobacillus-spp
#11
Leann F Bacon, Charlotte Hamley-Bennett, Michael J Danson, David J Leak
BACKGROUND: Geobacillus thermoglucosidasius is a thermophilic, natural ethanol producer and a potential candidate for commercial bioethanol production. Previously, G. thermoglucosidasius has been genetically modified to create an industrially-relevant ethanol production strain. However, creating chromosomal integrations and deletions in Geobacillus spp. is laborious. Here we describe a new technique to create marker-less mutations in Geobacillus utilising a novel homologous recombination process...
April 5, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28376880/a-review-on-lactococcus-lactis-from-food-to-factory
#12
REVIEW
Adelene Ai-Lian Song, Lionel L A In, Swee Hua Erin Lim, Raha Abdul Rahim
Lactococcus lactis has progressed a long way since its discovery and initial use in dairy product fermentation, to its present biotechnological applications in genetic engineering for the production of various recombinant proteins and metabolites that transcends the heterologous species barrier. Key desirable features of this gram-positive lactic acid non-colonizing gut bacteria include its generally recognized as safe (GRAS) status, probiotic properties, the absence of inclusion bodies and endotoxins, surface display and extracellular secretion technology, and a diverse selection of cloning and inducible expression vectors...
April 4, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28376879/cell-surface-engineering-of-bacillus-subtilis-improves-production-yields-of-heterologously-expressed-%C3%AE-amylases
#13
Haojie Cao, Auke J van Heel, Hifza Ahmed, Maarten Mols, Oscar P Kuipers
BACKGROUND: Bacillus subtilis is widely used as a cell factory for numerous heterologous proteins of commercial value and medical interest. To explore the possibility of further enhancing the secretion potential of this model bacterium, a library of engineered strains with modified cell surface components was constructed, and the corresponding influences on protein secretion were investigated by analyzing the secretion of α-amylase variants with either low-, neutral- or high- isoelectric points (pI)...
April 4, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28376795/intramembrane-protease-rasp-boosts-protein-production-in-bacillus
#14
Jolanda Neef, Cristina Bongiorni, Vivianne J Goosens, Brian Schmidt, Jan Maarten van Dijl
BACKGROUND: The microbial cell factory Bacillus subtilis is a popular industrial platform for high-level production of secreted technical enzymes. Nonetheless, the effective secretion of particular heterologous enzymes remains challenging. Over the past decades various studies have tackled this problem, and major improvements were achieved by optimizing signal peptides or removing proteases involved in product degradation. On the other hand, serious bottlenecks in the protein export process per se remained enigmatic, especially for protein secretion at commercially significant levels by cells grown to high density...
April 4, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28356104/heterologous-biosynthesis-and-manipulation-of-crocetin-in-saccharomyces-cerevisiae
#15
Fenghua Chai, Ying Wang, Xueang Mei, Mingdong Yao, Yan Chen, Hong Liu, Wenhai Xiao, Yingjin Yuan
BACKGROUND: Due to excellent performance in antitumor, antioxidation, antihypertension, antiatherosclerotic and antidepressant activities, crocetin, naturally exists in Crocus sativus L., has great potential applications in medical and food fields. Microbial production of crocetin has received increasing concern in recent years. However, only a patent from EVOVA Inc. and a report from Lou et al. have illustrated the feasibility of microbial biosynthesis of crocetin, but there was no specific titer data reported so far...
March 29, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28351356/erratum-to-switch-a-dynamic-crispr-tool-for-genome-engineering-and-metabolic-pathway-control-for-cell-factory-construction-in-saccharomyces-cerevisiae
#16
Katherina García Vanegas, Beata Joanna Lehka, Uffe Hasbro Mortensen
No abstract text is available yet for this article.
March 28, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28347340/expanding-metabolic-pathway-for-de-novo-biosynthesis-of-the-chiral-pharmaceutical-intermediate-l-pipecolic-acid-in-escherichia-coli
#17
Hanxiao Ying, Sha Tao, Jing Wang, Weichao Ma, Kequan Chen, Xin Wang, Pingkai Ouyang
BACKGROUND: The six-carbon circular non-proteinogenic compound L-pipecolic acid is an important chiral drug intermediate with many applications in the pharmaceutical industry. In the present study, we developed a metabolically engineered strain of Escherichia coli for the overproduction of L-pipecolic acid from glucose. RESULTS: The metabolic pathway from L-lysine to L-pipecolic acid was constructed initially by introducing lysine cyclodeaminase (LCD). Next, L-lysine metabolic flux from glucose was amplified by the plasmid-based overexpression of dapA, lysC, and lysA under the control of the strong trc promoter to increase the biosynthetic pool of the precursor L-lysine...
March 27, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28335772/production-of-s-2-aminobutyric-acid-and-s-2-aminobutanol-in-saccharomyces-cerevisiae
#18
Nora Weber, Anaëlle Hatsch, Ludivine Labagnere, Harald Heider
BACKGROUND: Saccharomyces cerevisiae (baker's yeast) has great potential as a whole-cell biocatalyst for multistep synthesis of various organic molecules. To date, however, few examples exist in the literature of the successful biosynthetic production of chemical compounds, in yeast, that do not exist in nature. Considering that more than 30% of all drugs on the market are purely chemical compounds, often produced by harsh synthetic chemistry or with very low yields, novel and environmentally sound production routes are highly desirable...
March 23, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28320377/application-of-an-e-coli-signal-sequence-as-a-versatile-inclusion-body-tag
#19
Wouter S P Jong, David Vikström, Diane Houben, H Bart van den Berg van Saparoea, Jan-Willem de Gier, Joen Luirink
BACKGROUND: Heterologous protein production in Escherichia coli often suffers from bottlenecks such as proteolytic degradation, complex purification procedures and toxicity towards the expression host. Production of proteins in an insoluble form in inclusion bodies (IBs) can alleviate these problems. Unfortunately, the propensity of heterologous proteins to form IBs is variable and difficult to predict. Hence, fusing the target protein to an aggregation prone polypeptide or IB-tag is a useful strategy to produce difficult-to-express proteins in an insoluble form...
March 21, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28302114/implications-of-evolutionary-engineering-for-growth-and-recombinant-protein-production-in-methanol-based-growth-media-in-the-yeast-pichia-pastoris
#20
Josef W Moser, Roland Prielhofer, Samuel M Gerner, Alexandra B Graf, Iain B H Wilson, Diethard Mattanovich, Martin Dragosits
BACKGROUND: Pichia pastoris is a widely used eukaryotic expression host for recombinant protein production. Adaptive laboratory evolution (ALE) has been applied in a wide range of studies in order to improve strains for biotechnological purposes. In this context, the impact of long-term carbon source adaptation in P. pastoris has not been addressed so far. Thus, we performed a pilot experiment in order to analyze the applicability and potential benefits of ALE towards improved growth and recombinant protein production in P...
March 17, 2017: Microbial Cell Factories
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