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Microbial Cell Factories

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https://www.readbyqxmd.com/read/28629408/biochemical-characterization-of-an-enantioselective-esterase-from-brevundimonas-sp-ly-2
#1
Jing Zhang, Mengjun Zhao, Die Yu, Jingang Yin, Hao Zhang, Xing Huang
BACKGROUND: Lactofen, a member of the diphenylether herbicides, has high activity and is commonly used to control broadleaf weeds. As a post-emergent herbicide, it is directly released to the environment, and easily caused the pollution. This herbicide is degraded in soil mainly by microbial activity, but the functional enzyme involved in the biodegradation of lactofen is still not clear now. RESULTS: A novel esterase gene lacH, involved in the degradation of lactofen, was cloned from the strain Brevundimonas sp...
June 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28619110/effect-of-aspartic-acid-and-glutamate-on-metabolism-and-acid-stress-resistance-of-acetobacter-pasteurianus
#2
Haisong Yin, Renkuan Zhang, Menglei Xia, Xiaolei Bai, Jun Mou, Yu Zheng, Min Wang
BACKGROUND: Acetic acid bacteria (AAB) are widely applied in food, bioengineering and medicine fields. However, the acid stress at low pH conditions limits acetic acid fermentation efficiency and high concentration of vinegar production with AAB. Therefore, how to enhance resistance ability of the AAB remains as the major challenge. Amino acids play an important role in cell growth and cell survival under severe environment. However, until now the effects of amino acids on acetic fermentation and acid stress resistance of AAB have not been fully studied...
June 15, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28619052/heterologous-expression-of-an-%C3%AE-amylase-inhibitor-from-common-bean-phaseolus-vulgaris-in-kluyveromyces-lactis-and-saccharomyces-cerevisiae
#3
Stephanie Brain-Isasi, Alejandro Álvarez-Lueje, Thomas Joseph V Higgins
BACKGROUND: Phaseolamin or α-amylase inhibitor 1 (αAI) is a glycoprotein from common beans (Phaseolus vulgaris L.) that inhibits some insect and mammalian α-amylases. Several clinical studies support the beneficial use of bean αAI for control of diabetes and obesity. Commercial extracts of P. vulgaris are available but their efficacy is still under question, mainly because some of these extracts contain antinutritional impurities naturally present in bean seeds and also exhibit a lower specific activity αAI...
June 15, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28619035/an-evolutionary-optimization-of-a-rhodopsin-based-phototrophic-metabolism-in-escherichia-coli
#4
Hyun Aaron Kim, Hyun Ju Kim, Jihoon Park, Ah Reum Choi, Kyoo Heo, Haeyoung Jeong, Kwang-Hwan Jung, Yeong-Jae Seok, Pil Kim, Sang Jun Lee
BACKGROUND: The expression of the Gloeobacter rhodopsin (GR) in a chemotrophic Escherichia coli enables the light-driven phototrophic energy generation. Adaptive laboratory evolution has been used for acquiring desired phenotype of microbial cells and for the elucidation of basic mechanism of molecular evolution. To develop an optimized strain for the artificially acquired phototrophic metabolism, an ancestral E. coli expressing GR was adaptively evolved in a chemostat reactor with constant illumination and limited glucose conditions...
June 15, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28619018/efficient-soluble-expression-of-disulfide-bonded-proteins-in-the-cytoplasm-of-escherichia-coli-in-fed-batch-fermentations-on-chemically-defined-minimal-media
#5
Anna Gąciarz, Narendar Kumar Khatri, M Lourdes Velez-Suberbie, Mirva J Saaranen, Yuko Uchida, Eli Keshavarz-Moore, Lloyd W Ruddock
BACKGROUND: The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in the cytoplasm in the form of inclusion bodies, then solubilized and re-folded in vitro. Both of these approaches have limitations. Previously we showed that soluble expression of disulfide bonded proteins in the cytoplasm of E. coli is possible at shake flask scale with a system, known as CyDisCo, which is based on co-expression of a protein of interest along with a sulfhydryl oxidase and a disulfide bond isomerase...
June 15, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28615057/erratum-to-engineering-an-efficient-and-tight-d-amino-acid-inducible-gene-expression-system-in-rhodosporidium-rhodotorula-species
#6
Yanbin Liu, Chong Mei John Koh, Si Te Ngoh, Lianghui Ji
No abstract text is available yet for this article.
June 14, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28610636/whole-cell-biocatalysts-by-design
#7
REVIEW
Baixue Lin, Yong Tao
Whole-cell biocatalysts provide unique advantages and have been widely used for the efficient biosynthesis of value-added fine and bulk chemicals, as well as pharmaceutically active ingredients. What is more, advances in synthetic biology and metabolic engineering, together with the rapid development of molecular genetic tools, have brought about a renaissance of whole-cell biocatalysis. These rapid advancements mean that whole-cell biocatalysts can increasingly be rationally designed. Genes of heterologous enzymes or synthetic pathways are increasingly being introduced into microbial hosts, and depending on the complexity of the synthetic pathway or the target products, they can enable the production of value-added chemicals from cheap feedstock...
June 13, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28610588/cell-foundry-with-high-product-specificity-and-catalytic-activity-for-21-deoxycortisol-biotransformation
#8
Shuting Xiong, Ying Wang, Mingdong Yao, Hong Liu, Xiao Zhou, Wenhai Xiao, Yingjin Yuan
BACKGROUND: 21-deoxycortisol (21-DF) is the key intermediate to manufacture pharmaceutical glucocorticoids. Recently, a Japan patent has realized 21-DF production via biotransformation of 17-hydroxyprogesterone (17-OHP) by purified steroid 11β-hydroxylase CYP11B1. Due to the less costs on enzyme isolation, purification and stabilization as well as cofactors supply, whole-cell should be preferentially employed as the biocatalyst over purified enzymes. No reports as so far have demonstrated a whole-cell system to produce 21-DF...
June 13, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28606152/heterologous-expression-of-cytotoxic-sesquiterpenoids-from-the-medicinal-mushroom-lignosus-rhinocerotis-in-yeast
#9
Hui-Yeng Yeannie Yap, Mariano Jordi Muria-Gonzalez, Boon-Hong Kong, Keith A Stubbs, Chon-Seng Tan, Szu-Ting Ng, Nget-Hong Tan, Peter S Solomon, Shin-Yee Fung, Yit-Heng Chooi
BACKGROUND: Genome mining facilitated by heterologous systems is an emerging approach to access the chemical diversity encoded in basidiomycete genomes. In this study, three sesquiterpene synthase genes, GME3634, GME3638, and GME9210, which were highly expressed in the sclerotium of the medicinal mushroom Lignosus rhinocerotis, were cloned and heterologously expressed in a yeast system. RESULTS: Metabolite profile analysis of the yeast culture extracts by GC-MS showed the production of several sesquiterpene alcohols (C15H26O), including cadinols and germacrene D-4-ol as major products...
June 12, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28606145/three-steps-in-one-pot-biosynthesis-of-4-hydroxycinnamyl-alcohols-using-immobilized-whole-cells-of-two-genetically-engineered-escherichia-coli-strains
#10
Shuxin Liu, Jiabin Liu, Jiayin Hou, Nan Chao, Ying Gai, Xiangning Jiang
BACKGROUND: 4-Hydroxycinnamyl alcohols are a class of natural plant secondary metabolites that include p-coumaryl alcohol, caffeyl alcohol, coniferyl alcohol and sinapyl alcohol, and have physiological, ecological and biomedical significance. While it is necessary to investigate the biological pathways and economic value of these alcohols, research is hindered because of their limited availability and high cost. Traditionally, these alcohols are obtained by chemical synthesis and plant extraction...
June 12, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28606142/erratum-to-expression-of-the-clonostachys-rosea-lactonohydrolase-gene-by-lactobacillus-reuteri-to-increase-its-zearalenone-removing-ability
#11
Wen-Chun Yang, Tsui-Chun Hsu, Kuan-Chen Cheng, Je-Ruei Liu
No abstract text is available yet for this article.
June 12, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28599654/identification-of-nocamycin-biosynthetic-gene-cluster-from-saccharothrix-syringae-nrrl-b-16468-and-generation-of-new-nocamycin-derivatives-by-manipulating-gene-cluster
#12
Xuhua Mo, Chunrong Shi, Chun Gui, Yanjiao Zhang, Jianhua Ju, Qingji Wang
BACKGROUND: Nocamycins I and II, produced by the rare actinomycete Saccharothrix syringae, belong to the tetramic acid family natural products. Nocamycins show potent antimicrobial activity and they hold great potential for antibacterial agent design. However, up to now, little is known about the exact biosynthetic mechanism of nocamycin. RESULTS: In this report, we identified the gene cluster responsible for nocamycin biosynthesis from S. syringae and generated new nocamycin derivatives by manipulating its gene cluster...
June 9, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28599651/microtiter-plate-cultivation-of-oleaginous-fungi-and-monitoring-of-lipogenesis-by-high-throughput-ftir-spectroscopy
#13
Gergely Kosa, Achim Kohler, Valeria Tafintseva, Boris Zimmermann, Kristin Forfang, Nils Kristian Afseth, Dimitrios Tzimorotas, Kiira S Vuoristo, Svein Jarle Horn, Jerome Mounier, Volha Shapaval
BACKGROUND: Oleaginous fungi can accumulate lipids by utilizing a wide range of waste substrates. They are an important source for the industrial production of omega-6 polyunsaturated fatty acids (gamma-linolenic and arachidonic acid) and have been suggested as an alternative route for biodiesel production. Initial research steps for various applications include the screening of fungi in order to find efficient fungal producers with desired fatty acid composition. Traditional cultivation methods (shake flask) and lipid analysis (extraction-gas chromatography) are not applicable for large-scale screening due to their low throughput and time-consuming analysis...
June 9, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28595601/multicopy-plasmid-integration-in-komagataella-phaffii-mediated-by-a-defective-auxotrophic-marker
#14
Maritza Ocampo Betancur, Viviane Castelo Branco Reis, André Moraes Nicola, Janice Lisboa De Marco, Lídia Maria Pepe de Moraes, Fernando Araripe Gonçalves Torres
BACKGROUND: A commonly used approach to improve recombinant protein production is to increase the levels of expression by providing extra-copies of a heterologous gene. In Komagataella phaffii (Pichia pastoris) this is usually accomplished by transforming cells with an expression vector carrying a drug-resistance marker following a screening for multicopy clones on plates with increasingly higher concentrations of an antibiotic. Alternatively, defective auxotrophic markers can be used for the same purpose...
June 8, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28587617/construction-of-energy-conserving-sucrose-utilization-pathways-for-improving-poly-%C3%AE-glutamic-acid-production-in-bacillus-amyloliquefaciens
#15
Jun Feng, Yanyan Gu, Yufen Quan, Weixia Gao, Yulei Dang, Mingfeng Cao, Xiaoyun Lu, Yi Wang, Cunjiang Song, Shufang Wang
BACKGROUND: Sucrose is an naturally abundant and easily fermentable feedstock for various biochemical production processes. By now, several sucrose utilization pathways have been identified and characterized. Among them, the pathway consists of sucrose permease and sucrose phosphorylase is an energy-conserving sucrose utilization pathway because it consumes less ATP when comparing to other known pathways. Bacillus amyloliquefaciens NK-1 strain can use sucrose as the feedstock to produce poly-γ-glutamic acid (γ-PGA), a highly valuable biopolymer...
June 6, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28569168/construct-design-production-and-characterization-of-plasmodium-falciparum-48-45-r0-6c-subunit-protein-produced-in-lactococcus-lactis-as-candidate-vaccine
#16
Susheel K Singh, Will Roeffen, Ulrik H Mistarz, Bishwanath Kumar Chourasia, Fen Yang, Kasper D Rand, Robert W Sauerwein, Michael Theisen
BACKGROUND: The sexual stages of Plasmodium falciparum are responsible for the spread of the parasite in malaria endemic areas. The cysteine-rich Pfs48/45 protein, exposed on the surface of sexual stages, is one of the most advanced antigens for inclusion into a vaccine that will block transmission. However, clinical Pfs48/45 sub-unit vaccine development has been hampered by the inability to produce high yields of recombinant protein as the native structure is required for the induction of functional transmission-blocking (TB) antibodies...
May 31, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28569150/heterologous-expression-of-pikromycin-biosynthetic-gene-cluster-using-streptomyces-artificial-chromosome-system
#17
Hye-Rim Pyeon, Hee-Ju Nah, Seung-Hoon Kang, Si-Sun Choi, Eung-Soo Kim
BACKGROUND: Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully applied for precise cloning and tandem integration of a large polyketide tautomycetin (TMC) biosynthetic gene cluster (Nah et al. in Microb Cell Fact 14(1):1, 2015), implying that this strategy could be employed to develop a custom overexpression scheme of natural product pathway clusters present in actinomycetes...
May 31, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28558761/production-characterization-and-antifungal-activity-of-a-biosurfactant-produced-by-rhodotorula-babjevae-ys3
#18
Suparna Sen, Siddhartha Narayan Borah, Arijit Bora, Suresh Deka
BACKGROUND: Sophorolipids are one of the most promising glycolipid biosurfactants and have been successfully employed in bioremediation and various other industrial sectors. They have also been described to exhibit antimicrobial activity against different bacterial species. Nevertheless, previous literature pertaining to the antifungal activity of sophorolipids are limited indicating the need for further research to explore novel strains with wide antimicrobial activity. A novel yeast strain, Rhodotorula babjevae YS3, was recently isolated from an agricultural field in Assam, Northeast India...
May 30, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28545544/exploring-the-biocombinatorial-potential-of-benzoxazoles-generation-of-novel-caboxamycin-derivatives
#19
Armando A Losada, Carmen Méndez, José A Salas, Carlos Olano
BACKGROUND: The biosynthesis pathway of benzoxazole compounds caboxamycin and nataxazole have been recently elucidated. Both compounds share one of their precursors, 3-hydroxyanthranilate (two units in the case of nataxazole). In addition, caboxamycin structure includes a salicylate moiety while 6-methylsalycilate is the third scaffold in nataxazole. Pathways cross-talk has been identified in caboxamycin producer Streptomyces sp. NTK937, between caboxamycin and enterobactin pathways, and nataxazole producer Streptomyces sp...
May 25, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28545467/expression-of-deleted-atoxic-atypical-recombinant-beta2-toxin-in-a-baculovirus-system-and-production-of-polyclonal-and-monoclonal-antibodies
#20
Anna Serroni, Chiara Francesca Magistrali, Giovanni Pezzotti, Luca Bano, Martina Pellegrini, Giulio Severi, Chiara Di Pancrazio, Mirella Luciani, Manuela Tittarelli, Silvia Tofani, Antonio De Giuseppe
BACKGROUND: Clostridium perfringens is an important animal and human pathogen that can produce more than 16 different major and minor toxins. The beta-2 minor toxin (CPB2), comprising atypical and consensus variants, appears to be involved in both human and animal enterotoxaemia syndrome. The exact role of CPB2 in pathogenesis is poorly investigated, and its mechanism of action at the molecular level is still unknown because of the lack of specific reagents such as monoclonal antibodies against the CPB2 protein and/or the availability of a highly purified antigen...
May 25, 2017: Microbial Cell Factories
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