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Microbial Cell Factories

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https://www.readbyqxmd.com/read/29778095/establishing-an-innovative-carbohydrate-metabolic-pathway-for-efficient-production-of-2-keto-l-gulonic-acid-in-ketogulonicigenium-robustum-initiated-by-intronic-promoters
#1
Cai-Yun Wang, Ye Li, Zi-Wei Gao, Li-Cheng Liu, Meng-Yue Zhang, Tian-Yuan Zhang, Chun-Fu Wu, Yi-Xuan Zhang
BACKGROUND: 2-Keto-L-gulonic acid (2-KGA), the precursor of vitamin C, is currently produced by two-step fermentation. In the second step, L-sorbose is transformed into 2-KGA by the symbiosis system composed of Ketogulonicigenium vulgare and Bacillus megaterium. Due to the different nutrient requirements and the uncertain ratio of the two strains, the symbiosis system significantly limits strain improvement and fermentation optimization. RESULTS: In this study, Ketogulonicigenium robustum SPU_B003 was reported for its capability to grow well independently and to produce more 2-KGA than that of K...
May 19, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29778094/substrate-sustained-release-based-high-efficacy-biosynthesis-of-gaba-by-lactobacillus-brevis-ncl912
#2
Qiong Wang, Xiaohua Liu, Jinheng Fu, Shuixing Wang, Yuanhong Chen, Kunpeng Chang, Haixing Li
BACKGROUND: Gamma-aminobutyric acid (GABA) plays a significant role in the food and drug industries. Our previous study established an efficient fed-batch fermentation process for Lactobacillus brevis NCL912 production of GABA from monosodium L-glutamate; however, monosodium L-glutamate may not be an ideal substrate, as it can result in the rapid increase of pH due to decarboxylation. Thus, in this study, L-glutamic acid was proposed as a substrate. To evaluate its potential, key components of the fermentation medium affecting GABA synthesis were re-screened and re-optimized to enhance GABA production from L...
May 19, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29778093/bio-detheobromination-of-cocoa-pod-husks-reduction-of-ochratoxin-a-content-without-change-in-nutrient-profile
#3
Daniel Oduro-Mensah, Augustine Ocloo, Sammy T Lowor, Cheetham Mingle, Laud K N-A Okine, Naa Ayikailey Adamafio
BACKGROUND: Utilization of cocoa pod husks (CPH) in animal feed is hindered by the presence of theobromine, which is variably toxic to animals. Treatment of this agro-waste to remove theobromine, while preserving its nutrient content, would allow beneficial use of the millions of metric tonnes discarded annually. The aim of this study was to assess the suitability of selected theobromine-degrading filamentous fungi for use as bio-tools in degradation of theobromine in CPH. RESULTS: The candidate fungi assessed in this study were an Aspergillus niger (AnTD) and three Talaromyces spp...
May 19, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29776370/characterization-of-a-promiscuous-cadmium-and-arsenic-resistance-mechanism-in-thermus-thermophilus-hb27-and-potential-application-of-a-novel-bioreporter-system
#4
Immacolata Antonucci, Giovanni Gallo, Danila Limauro, Patrizia Contursi, Ana Luisa Ribeiro, Alba Blesa, José Berenguer, Simonetta Bartolucci, Gabriella Fiorentino
BACKGROUND: The characterization of the molecular determinants of metal resistance has potential biotechnological application in biosensing and bioremediation. In this context, the bacterium Thermus thermophilus HB27 is a metal tolerant thermophile containing a set of genes involved in arsenic resistance which, differently from other microbes, are not organized into a single operon. They encode the proteins: arsenate reductase, TtArsC, arsenic efflux membrane transporter, TtArsX, and transcriptional repressor, TtSmtB...
May 18, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29776364/identification-function-and-application-of-3-ketosteroid-%C3%AE-1-dehydrogenase-isozymes-in-mycobacterium-neoaurum-dsm-1381-for-the-production-of-steroidic-synthons
#5
Ruijie Zhang, Xiangcen Liu, Yushi Wang, Yuchang Han, Junsong Sun, Jiping Shi, Baoguo Zhang
BACKGROUND: 3-Ketosteroid-Δ1-dehydrogenase (KstD) is a key enzyme in the metabolic pathway for chemical modifications of steroid hormones. Only a few KstDs have thus far been characterized biochemically and applied for the production of steroidal pharmaceutical intermediates. Three KstDs, KstD1, KstD2, and KstD3, were identified in Mycobacterium neoaurum DSM 1381, and they shared up to 99, 85 and 97% amino acid identity with previously reported KstDs, respectively. In this paper, KstDs from M...
May 18, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29773074/enhanced-cellulase-production-in-trichoderma-reesei-rut-c30-via-constitution-of-minimal-transcriptional-activators
#6
Jiajia Zhang, Guoxiu Zhang, Wei Wang, Wei Wang, Dongzhi Wei
BACKGROUND: Cellulase can convert lignocellulosic feedstocks into fermentable sugars, which can be used for the industrial production of biofuels and chemicals. The high cost of cellulase production remains a challenge for lignocellulose breakdown. Trichoderma reesei RUT C30 serves as a well-known industrial workhorse for cellulase production. Therefore, the enhancement of cellulase production by T. reesei RUT C30 is of great importance. RESULTS: Two sets of novel minimal transcriptional activators (DBDace2 -VP16 and DBDcre1 -VP16) were designed and expressed in T...
May 17, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29773073/metabolome-analysis-based-design-and-engineering-of-a-metabolic-pathway-in-corynebacterium-glutamicum-to-match-rates-of-simultaneous-utilization-of-d-glucose-and-l-arabinose
#7
Hideo Kawaguchi, Kumiko Yoshihara, Kiyotaka Y Hara, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
BACKGROUND: L-Arabinose is the second most abundant component of hemicellulose in lignocellulosic biomass, next to D-xylose. However, few microorganisms are capable of utilizing pentoses, and catabolic genes and operons enabling bacterial utilization of pentoses are typically subject to carbon catabolite repression by more-preferred carbon sources, such as D-glucose, leading to a preferential utilization of D-glucose over pentoses. In order to simultaneously utilize both D-glucose and L-arabinose at the same rate, a modified metabolic pathway was rationally designed based on metabolome analysis...
May 17, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29764434/real-time-monitoring-of-the-budding-index-in-saccharomyces-cerevisiae-batch-cultivations-with-in-situ-microscopy
#8
Anna-Maria Marbà-Ardébol, Jörn Emmerich, Michael Muthig, Peter Neubauer, Stefan Junne
BACKGROUND: The morphology of yeast cells changes during budding, depending on the growth rate and cultivation conditions. A photo-optical microscope was adapted and used to observe such morphological changes of individual cells directly in the cell suspension. In order to obtain statistically representative samples of the population without the influence of sampling, in situ microscopy (ISM) was applied in the different phases of a Saccharomyces cerevisiae batch cultivation. The real-time measurement was performed by coupling a photo-optical probe to an automated image analysis based on a neural network approach...
May 15, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29764418/engineering-xylose-metabolism-for-production-of-polyhydroxybutyrate-in-the-non-model-bacterium-burkholderia-sacchari
#9
Linda P Guamán, Carlos Barba-Ostria, Fuzhong Zhang, Edmar R Oliveira-Filho, José Gregório C Gomez, Luiziana F Silva
BACKGROUND: Despite its ability to grow and produce high-value molecules using renewable carbon sources, two main factors must be improved to use Burkholderia sacchari as a chassis for bioproduction at an industrial scale: first, the lack of molecular tools to engineer this organism and second, the inherently slow growth rate and poly-3-hydroxybutyrate [P(3HB)] production using xylose. In this work, we have addressed both factors. RESULTS: First, we adapted a set of BglBrick plasmids and showed tunable expression in B...
May 15, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29753327/corynebacterium-glutamicum-as-platform-for-the-production-of-hydroxybenzoic-acids
#10
Nicolai Kallscheuer, Jan Marienhagen
BACKGROUND: Hydroxybenzoic acids are industrially relevant aromatic compounds, which also play key roles in the microbial carbon metabolism, e.g., as precursors for the synthesis of cofactors or metal-chelating molecules. Due to its pronounced resistance to aromatics Corynebacterium glutamicum represents an interesting platform for production of these compounds. Unfortunately, a complex catabolic network for aromatic molecules prevents application of C. glutamicum for microbial production of aromatic compounds other than aromatic amino acids, which cannot be metabolized by this microorganism...
May 12, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29753326/biosynthesis-of-angelyl-coa-in-saccharomyces-cerevisiae
#11
Roberta Callari, David Fischer, Harald Heider, Nora Weber
BACKGROUND: The angelic acid moiety represents an essential modification in many biologically active products. These products are commonly known as angelates and several studies have demonstrated their therapeutic benefits, including anti-inflammatory and anti-cancer effects. However, their availability for use in the development of therapeutics is limited due to poor extraction yields. Chemical synthesis has been achieved but its complexity prevents application, therefore microbial production may offer a promising alternative...
May 12, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29753319/biotransformation-of-steroids-by-entomopathogenic-strains-of-isaria-farinosa
#12
Ewa Kozłowska, Natalia Hoc, Jordan Sycz, Monika Urbaniak, Monika Dymarska, Jakub Grzeszczuk, Edyta Kostrzewa-Susłow, Łukasz Stępień, Elżbieta Pląskowska, Tomasz Janeczko
BACKGROUND: Steroid compounds are very interesting substrates for biotransformation due to their high biological activity and a high number of inactivated carbons which make chemical modification difficult. Microbial transformation can involve reactions which are complicated and uneconomical in chemical synthesis, and searching for a new effective biocatalyst is necessary. The best known entomopathogenic species used in steroid modification is Beauveria bassiana. In this study we tested the ability of Isaria farinosa, another entomopathogenic species, to transform several steroids...
May 12, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29743073/parallel-substrate-supply-and-ph-stabilization-for-optimal-screening-of-e-coli-with-the-membrane-based-fed-batch-shake-flask
#13
P Philip, D Kern, J Goldmanns, F Seiler, A Schulte, T Habicher, J Büchs
BACKGROUND: Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism. As a screening tool on shake flask level, the membrane-based fed-batch shake flask was developed. While a controlled supply of a substrate was realized with the in-built membrane tip, the possibilities for replenishing nutrients and stabilizing pH values was not yet exploited...
May 9, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29729667/bioprospection-of-actinobacteria-derived-from-freshwater-sediments-for-their-potential-to-produce-antimicrobial-compounds
#14
Zothanpuia, Ajit Kumar Passari, Vincent Vineeth Leo, Preeti Chandra, Brijesh Kumar, Chandra Nayak, Abeer Hashem, Elsayed Fathi Abd Allah, Abdulaziz A Alqarawi, Bhim Pratap Singh
BACKGROUND: Actinobacteria from freshwater habitats have been explored less than from other habitats in the search for compounds of pharmaceutical value. This study highlighted the abundance of actinobacteria from freshwater sediments of two rivers and one lake, and the isolates were studied for their ability to produce antimicrobial bioactive compounds. RESULTS: 16S rRNA gene sequencing led to the identification of 84 actinobacterial isolates separated into a common genus (Streptomyces) and eight rare genera (Nocardiopsis, Saccharopolyspora, Rhodococcus, Prauserella, Amycolatopsis, Promicromonospora, Kocuria and Micrococcus)...
May 5, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29729665/metabolic-engineering-of-saccharomyces-cerevisiae-for-efficient-production-of-glucaric-acid-at-high-titer
#15
Na Chen, Jingya Wang, Yunying Zhao, Yu Deng
BACKGROUND: Glucaric acid is a high-value-added chemical that can be used in various fields. Because chemical oxidation of glucose to produce glucaric acid is not environmentally friendly, microbial production has attracted increasing interest recently. Biological pathways to synthesize glucaric acid from glucose in both Escherichia coli and Saccharomyces cerevisiae by co-expression of genes encoding myo-inositol-1-phosphate synthase (Ino1), myo-inositol oxygenase (MIOX), and uronate dehydrogenase (Udh) have been constructed...
May 5, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29720171/improved-ethanol-productivity-from-lignocellulosic-hydrolysates-by-escherichia-coli-with-regulated-glucose-utilization
#16
Jinfeng Sun, Kangming Tian, Jie Wang, Zixing Dong, Xiaoguang Liu, Kugenthiren Permaul, Suren Singh, Bernard A Prior, Zhengxiang Wang
BACKGROUND: Lignocellulosic ethanol could offer a sustainable source to meet the increasing worldwide demand for fuel. However, efficient and simultaneous metabolism of all types of sugars in lignocellulosic hydrolysates by ethanol-producing strains is still a challenge. RESULTS: An engineered strain Escherichia coli B0013-2021HPA with regulated glucose utilization, which could use all monosaccharides in lignocellulosic hydrolysates except glucose for cell growth and glucose for ethanol production, was constructed...
May 2, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29712559/heterologous-expression-of-spathaspora-passalidarum-xylose-reductase-and-xylitol-dehydrogenase-genes-improved-xylose-fermentation-ability-of-aureobasidium-pullulans
#17
Jian Guo, Siyao Huang, Yefu Chen, Xuewu Guo, Dongguang Xiao
BACKGROUND: Aureobasidium pullulans is a yeast-like fungus that can ferment xylose to generate high-value-added products, such as pullulan, heavy oil, and melanin. The combinatorial expression of two xylose reductase (XR) genes and two xylitol dehydrogenase (XDH) genes from Spathaspora passalidarum and the heterologous expression of the Piromyces sp. xylose isomerase (XI) gene were induced in A. pullulans to increase the consumption capability of A. pullulans on xylose. RESULTS: The overexpression of XYL1...
April 30, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29712558/enhancement-of-the-catalytic-activity-of-isopentenyl-diphosphate-isomerase-idi-from-saccharomyces-cerevisiae-through-random-and-site-directed-mutagenesis
#18
Hailin Chen, Meijie Li, Changqing Liu, Haibo Zhang, Mo Xian, Huizhou Liu
BACKGROUND: Lycopene is a terpenoid pigment that has diverse applications in the food and medicine industries. A prospective approach for lycopene production is by metabolic engineering in microbial hosts, such as Escherichia coli. Isopentenyl diphosphate isomerase (IDI, E.C. 5.3.3.2) is one of the rate-limiting enzymes in the lycopene biosynthetic pathway and one major target during metabolic engineering. The properties of IDIs differ depending on the sources, but under physiological conditions, IDIs are limited by low enzyme activity, short half-life and weak substrate affinity...
April 30, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29685154/a-recet-assisted-crispr-cas9-genome-editing-in-corynebacterium-glutamicum
#19
Bo Wang, Qitiao Hu, Yu Zhang, Ruilin Shi, Xin Chai, Zhe Liu, Xiuling Shang, Yun Zhang, Tingyi Wen
BACKGROUND: Extensive modification of genome is an efficient manner to regulate the metabolic network for producing target metabolites or non-native products using Corynebacterium glutamicum as a cell factory. Genome editing approaches by means of homologous recombination and counter-selection markers are laborious and time consuming due to multiple round manipulations and low editing efficiencies. The current two-plasmid-based CRISPR-Cas9 editing methods generate false positives due to the potential instability of Cas9 on the plasmid, and require a high transformation efficiency for co-occurrence of two plasmids transformation...
April 23, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29678175/gene-repression-via-multiplex-grna-strategy-in-y-lipolytica
#20
Jin-Lai Zhang, Yang-Zi Peng, Duo Liu, Hong Liu, Ying-Xiu Cao, Bing-Zhi Li, Chun Li, Ying-Jin Yuan
BACKGROUND: The oleaginous yeast Yarrowia lipolytica is a promising microbial cell factory due to their biochemical characteristics and native capacity to accumulate lipid-based chemicals. To create heterogenous biosynthesis pathway and manipulate metabolic flux in Y. lipolytica, numerous studies have been done for developing synthetic biology tools for gene regulation. CRISPR interference (CRISPRi), as an emerging technology, has been applied for specifically repressing genes of interest...
April 20, 2018: Microbial Cell Factories
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