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Microbial Cell Factories

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https://www.readbyqxmd.com/read/27912768/quasi-continuous-parallel-online-scattered-light-fluorescence-and-dissolved-oxygen-tension-measurement-combined-with-monitoring-of-the-oxygen-transfer-rate-in-each-well-of-a-shaken-microtiter-plate
#1
Tobias Ladner, Markus Held, David Flitsch, Mario Beckers, Jochen Büchs
BACKGROUND: Microtiter plates (MTP) are often applied as culture vessels in high-throughput screening programs. If online measuring techniques are available, MTPs can also be applied in the first steps of process development. For such small-scale bioreactors dipping probes are usually too large; therefore, optical measurements are often used. For example, the BioLector technology allows for the online monitoring of scattered light and fluorescence in each well of a continuously orbitally shaken MTP...
December 3, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27908280/development-of-a-fast-and-easy-method-for-escherichia-coli-genome-editing-with-crispr-cas9
#2
Dongdong Zhao, Shenli Yuan, Bin Xiong, Hongnian Sun, Lijun Ye, Jing Li, Xueli Zhang, Changhao Bi
BACKGROUND: Microbial genome editing is a powerful tool to modify chromosome in way of deletion, insertion or replacement, which is one of the most important techniques in metabolic engineering research. The emergence of CRISPR/Cas9 technique inspires various genomic editing methods. RESULTS: In this research, the goal of development of a fast and easy method for Escherichia coli genome editing with high efficiency is pursued. For this purpose, we designed modular plasmid assembly strategy, compared effects of different length of homologous arms for recombination, and tested different sets of recombinases...
December 1, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27905930/combinatory-optimization-of-chromosomal-integrated-mevalonate-pathway-for-%C3%AE-carotene-production-in-escherichia-coli
#3
Lijun Ye, Chunzhi Zhang, Changhao Bi, Qingyan Li, Xueli Zhang
BACKGROUND: Plasmid expression is a popular method in studies of MVA pathway for isoprenoid production in Escherichia coli. However, heterologous gene expression with plasmid is often not stable and might burden growth of host cells, decreases cell mass and product yield. In this study, MVA pathway was divided into three modules, and two heterologous modules were integrated into the E. coli chromosome. These modules were individually modulated with regulatory parts to optimize efficiency of the pathway in terms of downstream isoprenoid production...
December 1, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27905924/current-knowledge-and-perspectives-of-paenibacillus-a-review
#4
REVIEW
Elliot Nicholas Grady, Jacqueline MacDonald, Linda Liu, Alex Richman, Ze-Chun Yuan
Isolated from a wide range of sources, the genus Paenibacillus comprises bacterial species relevant to humans, animals, plants, and the environment. Many Paenibacillus species can promote crop growth directly via biological nitrogen fixation, phosphate solubilization, production of the phytohormone indole-3-acetic acid (IAA), and release of siderophores that enable iron acquisition. They can also offer protection against insect herbivores and phytopathogens, including bacteria, fungi, nematodes, and viruses...
December 1, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27894344/siropins-novel-serine-protease-inhibitors-from-gut-microbiota-acting-on-human-proteases-involved-in-inflammatory-bowel-diseases
#5
Héla Mkaouar, Nizar Akermi, Vincent Mariaule, Samira Boudebbouze, Nadia Gaci, Florette Szukala, Nicolas Pons, Josan Marquez, Ali Gargouri, Emmanuelle Maguin, Moez Rhimi
BACKGROUND: In eukaryotes, the serpins constitute a wide family of protease inhibitors regulating many physiological pathways. Many reports stressed the key role of serpins in several human physiopathologies including mainly the inflammatory bowel diseases. In this context, eukaryotic serpins were largely studied and their use to limit inflammation was reported. In comparison to that, bacterial serpins and mainly those from human gut microbiota remain poorly studied. RESULTS: The two genes encoding for putative serpins from the human gut bacterium Eubacterium sireaum, display low sequence identities...
November 29, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27887615/developing-a-set-of-strong-intronic-promoters-for-robust-metabolic-engineering-in-oleaginous-rhodotorula-rhodosporidium-yeast-species
#6
Yanbin Liu, Sihui Amy Yap, Chong Mei John Koh, Lianghui Ji
BACKGROUND: Red yeast species in the Rhodotorula/Rhodosporidium genus are outstanding producers of triacylglyceride and cell biomass. Metabolic engineering is expected to further enhance the productivity and versatility of these hosts for the production of biobased chemicals and fuels. Promoters with strong activity during oil-accumulation stage are critical tools for metabolic engineering of these oleaginous yeasts. RESULTS: The upstream DNA sequences of 6 genes involved in lipid biosynthesis or accumulation in Rhodotorula toruloides were studied by luciferase reporter assay...
November 25, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27876054/engineering-an-inducible-gene-expression-system-for-bacillus-subtilis-from-a-strong-constitutive-promoter-and-a-theophylline-activated-synthetic-riboswitch
#7
Wenjing Cui, Laichuang Han, Jintao Cheng, Zhongmei Liu, Li Zhou, Junling Guo, Zhemin Zhou
BACKGROUND: Synthetic riboswitches have been increasingly used to control and tune gene expression in diverse organisms. Although a set of theophylline-responsive riboswitches have been developed for bacteria, fully functional expression elements mediated by synthetic riboswitches in Bacillus subtilis are rarely used because of the host-dependent compatibility between the promoters and riboswitches. RESULTS: A novel genetic element composed of the promoter P43 and a theophylline-riboswitch was developed and characterized in B...
November 22, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27871334/exploiting-members-of-the-bahd-acyltransferase-family-to-synthesize-multiple-hydroxycinnamate-and-benzoate-conjugates-in-yeast
#8
Aymerick Eudes, Maxence Mouille, David S Robinson, Veronica T Benites, George Wang, Lucien Roux, Yi-Lin Tsai, Edward E K Baidoo, Tsan-Yu Chiu, Joshua L Heazlewood, Henrik V Scheller, Aindrila Mukhopadhyay, Jay D Keasling, Samuel Deutsch, Dominique Loqué
BACKGROUND: BAHD acyltransferases, named after the first four biochemically characterized enzymes of the group, are plant-specific enzymes that catalyze the transfer of coenzyme A-activated donors onto various acceptor molecules. They are responsible for the synthesis in plants of a myriad of secondary metabolites, some of which are beneficial for humans either as therapeutics or as specialty chemicals such as flavors and fragrances. The production of pharmaceutical, nutraceutical and commodity chemicals using engineered microbes is an alternative, green route to energy-intensive chemical syntheses that consume petroleum-based precursors...
November 21, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27863495/anaerobic-poly-3-d-hydroxybutyrate-production-from-xylose-in-recombinant-saccharomyces-cerevisiae-using-a-nadh-dependent-acetoacetyl-coa-reductase
#9
Alejandro Muñoz de Las Heras, Diogo J Portugal-Nunes, Nathasha Rizza, Anders G Sandström, Marie F Gorwa-Grauslund
BACKGROUND: Poly-3-D-hydroxybutyrate (PHB) that is a promising precursor for bioplastic with similar physical properties as polypropylene, is naturally produced by several bacterial species. The bacterial pathway is comprised of the three enzymes β-ketothiolase, acetoacetyl-CoA reductase (AAR) and PHB synthase, which all together convert acetyl-CoA into PHB. Heterologous expression of the pathway genes from Cupriavidus necator has enabled PHB production in the yeast Saccharomyces cerevisiae from glucose as well as from xylose, after introduction of the fungal xylose utilization pathway from Scheffersomyces stipitis including xylose reductase (XR) and xylitol dehydrogenase (XDH)...
November 18, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27846887/crispr-interference-crispri-for-gene-regulation-and-succinate-production-in-cyanobacterium-s-elongatus-pcc-7942
#10
Chun-Hung Huang, Claire R Shen, Hung Li, Li-Yu Sung, Meng-Ying Wu, Yu-Chen Hu
BACKGROUND: Cyanobacterium Synechococcus elongatus PCC 7942 holds promise for biochemical conversion, but gene deletion in PCC 7942 is time-consuming and may be lethal to cells. CRISPR interference (CRISPRi) is an emerging technology that exploits the catalytically inactive Cas9 (dCas9) and single guide RNA (sgRNA) to repress sequence-specific genes without the need of gene knockout, and is repurposed to rewire metabolic networks in various procaryotic cells. RESULTS: To employ CRISPRi for the manipulation of gene network in PCC 7942, we integrated the cassettes expressing enhanced yellow fluorescent protein (EYFP), dCas9 and sgRNA targeting different regions on eyfp into the PCC 7942 chromosome...
November 15, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27842593/easy-regulation-of-metabolic-flux-in-escherichia-coli-using-an-endogenous-type-i-e-crispr-cas-system
#11
Yizhao Chang, Tianyuan Su, Qingsheng Qi, Quanfeng Liang
BACKGROUND: Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is a recently developed powerful tool for gene regulation. In Escherichia coli, the type I CRISPR system expressed endogenously shall be easy for internal regulation without causing metabolic burden in compared with the widely used type II system, which expressed dCas9 as an additional plasmid. RESULTS: By knocking out cas3 and activating the expression of CRISPR-associated complex for antiviral defense (Cascade), we constructed a native CRISPRi system in E...
November 15, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27842591/regulation-of-carotenogenesis-in-the-red-yeast-xanthophyllomyces-dendrorhous-the-role-of-the-transcriptional-co-repressor-complex-cyc8-tup1-involved-in-catabolic-repression
#12
Pamela Córdova, Jennifer Alcaíno, Natalia Bravo, Salvador Barahona, Dionisia Sepúlveda, María Fernández-Lobato, Marcelo Baeza, Víctor Cifuentes
BACKGROUND: The yeast Xanthophyllomyces dendrorhous produces carotenoids of commercial interest, including astaxanthin and β-carotene. Although carotenogenesis in this yeast and the expression profiles of the genes controlling this pathway are known, the mechanisms regulating this process remain poorly understood. Several studies have demonstrated that glucose represses carotenogenesis in X. dendrorhous, suggesting that this pathway could be regulated by catabolic repression. Catabolic repression is a highly conserved regulatory mechanism in eukaryotes and has been widely studied in Saccharomyces cerevisiae...
November 14, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27842546/optimization-of-construct-design-and-fermentation-strategy-for-the-production-of-bioactive-atf-sap-a-saporin-based-anti-tumoral-upar-targeted-chimera
#13
Alfredo Errico Provenzano, Riccardo Posteri, Francesco Giansanti, Francesco Angelucci, Sopsamorn U Flavell, David J Flavell, Maria Serena Fabbrini, Danilo Porro, Rodolfo Ippoliti, Aldo Ceriotti, Paola Branduardi, Riccardo Vago
BACKGROUND: The big challenge in any anti-tumor therapeutic approach is represented by the development of drugs selectively acting on the target with limited side effects, that exploit the unique characteristics of malignant cells. The urokinase (urokinase-type plasminogen activator, uPA) and its receptor uPAR have been identified as preferential target candidates since they play a key role in the evolution of neoplasms and are associated with neoplasm aggressiveness and poor clinical outcome in several different tumor types...
November 14, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27835989/a-penicillium-chrysogenum-based-expression-system-for-the-production-of-small-cysteine-rich-antifungal-proteins-for-structural-and-functional-analyses
#14
Christoph Sonderegger, László Galgóczy, Sandra Garrigues, Ádám Fizil, Attila Borics, Paloma Manzanares, Nikoletta Hegedüs, Anna Huber, Jose F Marcos, Gyula Batta, Florentine Marx
BACKGROUND: Small, cysteine-rich and cationic antifungal proteins (APs) from filamentous ascomycetes, such as NFAP from Neosartorya fischeri and PAF from Penicillium chrysogenum, are promising candidates for novel drug development. A prerequisite for their application is a detailed knowledge about their structure-function relation and mode of action, which would allow protein modelling to enhance their toxicity and specificity. Technologies for structure analyses, such as electronic circular dichroism (ECD) or NMR spectroscopy, require highly purified samples and in case of NMR milligrams of uniformly (15)N-/(13)C-isotope labelled protein...
November 11, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27835967/carbonyl-reductase-identification-and-development-of-whole-cell-biotransformation-for-highly-efficient-synthesis-of-r-3-5-bis-trifluoromethyl-phenyl-ethanol
#15
Kangling Chen, Kefei Li, Jian Deng, Baoqi Zhang, Jinping Lin, Dongzhi Wei
BACKGROUND: (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol [(R)-3,5-BTPE] is a valuable chiral intermediate for Aprepitant (Emend) and Fosaprepitant (Ivemend). Biocatalyzed asymmetric reduction is a preferred approach to synthesize highly optically active (R)-3,5-BTPE. However, the product concentration and productivity of reported (R)-3,5-BTPE synthetic processes remain unsatisfied. RESULTS: A NADPH-dependent carbonyl reductase from Lactobacillus kefir (LkCR) was discovered by genome mining for reduction of 3,5-bis(trifluoromethyl) acetophenone (3,5-BTAP) into (R)-3,5-BTPE with excellent enantioselectivity...
November 11, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27832791/genetic-tools-for-advancement-of-synechococcus-sp-pcc-7002-as-a-cyanobacterial-chassis
#16
Anne M Ruffing, Travis J Jensen, Lucas M Strickland
BACKGROUND: Successful implementation of modified cyanobacteria as hosts for industrial applications requires the development of a cyanobacterial chassis. The cyanobacterium Synechococcus sp. PCC 7002 embodies key attributes for an industrial host, including a fast growth rate and high salt, light, and temperature tolerances. This study addresses key limitations in the advancement of Synechococcus sp. PCC 7002 as an industrial chassis. RESULTS: Tools for genome integration were developed and characterized, including several putative neutral sites for genome integration...
November 10, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27832790/erratum-to-protective-effect-of-tslp-delivered-at-the-gut-mucosa-level-by-recombinant-lactic-acid-bacteria-in-dss-induced-colitis-mouse-model
#17
Camille Aubry, Christophe Michon, Florian Chain, Yolande Chvatchko, Laurence Goffin, Simone C Zimmerli, Sylvia Leguin, Philippe Langella, Luis Bermudez-Humaran, Jean-Marc Chatel
No abstract text is available yet for this article.
November 10, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27832787/the-effect-of-protein-acetylation-on-the-formation-and-processing-of-inclusion-bodies-and-endogenous-protein-aggregates-in-escherichia-coli-cells
#18
Dorota Kuczyńska-Wiśnik, María Moruno-Algara, Karolina Stojowska-Swędrzyńska, Ewa Laskowska
BACKGROUND: Acetylation of lysine residues is a reversible post-translational modification conserved from bacteria to humans. Several recent studies have revealed hundreds of lysine-acetylated proteins in various bacteria; however, the physiological role of these modifications remains largely unknown. Since lysine acetylation changes the size and charge of proteins and thereby may affect their conformation, we assumed that lysine acetylation can stimulate aggregation of proteins, especially for overproduced recombinant proteins that form inclusion bodies...
November 10, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27829451/elucidation-of-the-glycosylation-steps-during-biosynthesis-of-antitumor-macrolides-pm100117-and-pm100118-and-engineering-for-novel-derivatives
#19
Raúl García Salcedo, Carlos Olano, Rogelio Fernández, Alfredo F Braña, Carmen Méndez, Fernando de la Calle, José A Salas
BACKGROUND: Antitumor compounds PM100117 and PM100118 are glycosylated polyketides derived from the marine actinobacteria Streptomyces caniferus GUA-06-05-006A. The organization and characterization of the PM100117/18 biosynthesis gene cluster has been recently reported. RESULTS: Based on the preceding information and new genetic engineering data, we have outlined the pathway by which PM100117/18 are glycosylated. Furthermore, these genetic engineering experiments have allowed the generation of novel PM100117/18 analogues...
November 9, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27825357/fermentative-production-and-direct-extraction-of-%C3%AE-bisabolol-in-metabolically-engineered-escherichia-coli
#20
Gui Hwan Han, Seong Keun Kim, Paul Kyung-Seok Yoon, Younghwan Kang, Byoung Su Kim, Yaoyao Fu, Bong Hyun Sung, Heung Chae Jung, Dae-Hee Lee, Seon-Won Kim, Seung-Goo Lee
BACKGROUND: (-)-α-Bisabolol, also known as levomenol, is an unsaturated sesquiterpene alcohol that has mainly been used in pharmaceutical and cosmetic products due to its anti-inflammatory and skin-soothing properties. (-)-α-Bisabolol is currently manufactured mainly by steam-distillation of the essential oils extracted from the Brazilian candeia tree that is under threat because its natural habitat is constantly shrinking. Therefore, microbial production of (-)-α-bisabolol plays a key role in the development of its sustainable production from renewable feedstock...
November 8, 2016: Microbial Cell Factories
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