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Microbial Cell Factories

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https://www.readbyqxmd.com/read/28526017/the-effect-of-hypoxia-on-the-lipidome-of-recombinant-pichia-pastoris
#1
Núria Adelantado, Pablo Tarazona, Karlheinz Grillitsch, Xavier García-Ortega, Sergi Monforte, Francisco Valero, Ivo Feussner, Günther Daum, Pau Ferrer
BACKGROUND: Cultivation of recombinant Pichia pastoris (Komagataella sp.) under hypoxic conditions has a strong positive effect on specific productivity when the glycolytic GAP promoter is used for recombinant protein expression, mainly due to upregulation of glycolytic conditions. In addition, transcriptomic analyses of hypoxic P. pastoris pointed out important regulation of lipid metabolism and unfolded protein response (UPR). Notably, UPR that plays a role in the regulation of lipid metabolism, amino acid metabolism and protein secretion, was found to be upregulated under hypoxia...
May 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28526016/efficient-extracellular-recombinant-production-and-purification-of-a-bacillus-cyclodextrin-glucanotransferase-in-escherichia-coli
#2
Christian Sonnendecker, Ren Wei, Elisabeth Kurze, Jinpeng Wang, Thorsten Oeser, Wolfgang Zimmermann
BACKGROUND: Cyclodextrin glucanotransferases (CGTases) catalyze the synthesis of cyclodextrins, cyclic oligosaccharides composed of glucose monomers that find applications in the pharmaceutical, food, and cosmetic industries. An economic application of these industrially important enzymes requires their efficient production and recovery. In this study, the effect of Sec-type signal peptides on the recombinant expression of a CGTase derived from Bacillus sp. G825-6 was investigated in Escherichia coli BL21(DE3) using a codon-adapted gene...
May 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28521794/in-vitro-synthesis-of-9-10-dihydroxyhexadecanoic-acid-using-recombinant-escherichia-coli
#3
Anees Kaprakkaden, Preeti Srivastava, Virendra Swarup Bisaria
BACKGROUND: Hydroxy fatty acids are widely used in food, chemical and cosmetic industries. A variety of dihydroxy fatty acids have been synthesized so far; however, no studies have been done on the synthesis of 9,10-dihydroxyhexadecanoic acid. In the present study recombinant E. coli has been used for the heterologous expression of fatty acid hydroxylating enzymes and the whole cell lysate of the induced culture was used for in vitro production of 9,10-dihydroxyhexadecanoic acid. RESULTS: A first of its kind proof of principle has been successfully demonstrated for the production of 9,10-dihydroxyhexadecanoic acid using three different enzymes viz...
May 18, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28511724/production-of-a-recombinant-swollenin-from-trichoderma-harzianum-in-escherichia-coli-and-its-potential-synergistic-role-in-biomass-degradation
#4
Clelton A Santos, Jaire A Ferreira-Filho, Anthonia O'Donovan, Vijai K Gupta, Maria G Tuohy, Anete P Souza
BACKGROUND: Fungal swollenins (SWOs) constitute a class of accessory proteins that are homologous to canonical plant expansins. Expansins and expansin-related proteins are well known for acting in the deagglomeration of cellulose structure by loosening macrofibrils. Consequently, SWOs can increase the accessibility and efficiency of the other enzymes involved in the saccharification of cellulosic substrates. Thus, SWOs are promising targets for improving the hydrolysis of plant biomass and for use as an additive to enhance the efficiency of an enzyme cocktail designed for the production of biofuels...
May 16, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28511681/chromosome-engineering-of-escherichia-coli-for-constitutive-production-of-salvianic-acid-a
#5
Liang Zhou, Qi Ding, Guo-Zhen Jiang, Zhen-Ning Liu, Hai-Yan Wang, Guang-Rong Zhao
BACKGROUND: Salvianic acid A (SAA), a valuable natural product from herbal plant Salvia miltiorrhiza, exhibits excellent antioxidant activities on food industries and efficacious therapeutic potential on cardiovascular diseases. Recently, production of SAA in engineered Escherichia coli was established via the artificial biosynthetic pathway of SAA on the multiple plasmids in our previous work. However, the plasmid-mediated system required to supplement expensive inducers and antibiotics during the fermentation process, restricting scale-up production of SAA...
May 16, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28494761/production-of-fuels-and-chemicals-from-xylose-by-engineered-saccharomyces-cerevisiae-a-review-and-perspective
#6
REVIEW
Suryang Kwak, Yong-Su Jin
Efficient xylose utilization is one of the most important pre-requisites for developing an economic microbial conversion process of terrestrial lignocellulosic biomass into biofuels and biochemicals. A robust ethanol producing yeast Saccharomyces cerevisiae has been engineered with heterologous xylose assimilation pathways. A two-step oxidoreductase pathway consisting of NAD(P)H-linked xylose reductase and NAD(+)-linked xylitol dehydrogenase, and one-step isomerase pathway using xylose isomerase have been employed to enable xylose assimilation in engineered S...
May 11, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28490329/directed-evolution-and-secretory-expression-of-a-pyrethroid-hydrolyzing-esterase-with-enhanced-catalytic-activity-and-thermostability
#7
Xiaolong Liu, Mingjun Liang, Yuhuan Liu, Xinjiong Fan
BACKGROUND: Pyrethroids are potentially harmful to human health and ecosystems. It is necessary to develop some efficient strategies to degrade pyrethroid residues. Biodegradation is generally considered as a safe, efficient, and inexpensive way to eliminate environmental contaminants. To date, although several pyrethroid-hydrolyzing esterases have been cloned, there has been no report about a pyrethroid hydrolase with high hydrolytic activity, good stability, and high productivity, indispensable enzymatic properties in practical biodegradation...
May 11, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28490371/purification-and-properties-of-a-novel-quizalofop-p-ethyl-hydrolyzing-esterase-involved-in-quizalofop-p-ethyl-degradation-by-pseudomonas-sp-j-2
#8
Hui Zhang, Mengya Li, Jie Li, Guangli Wang, Yuan Liu
Quizalofop-p-ethyl (QPE) is a post-emergence herbicide that effectively controls grass weeds and is often detected in the environment. However, the biochemical and molecular mechanisms of QPE degradation in the environment remains unclear. In this study, a highly effective QPE-degrading bacterial strain J-2 was isolated from acclimated activated sludge and identified as a Pseudomonas sp., containing the QPE breakdown metabolite quizalofop acid (QA) identified by Liquid Chromatography-Ion Trap-Mass Spectrometry (LC-IT-MS(n)) analysis...
May 10, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28482902/substrates-and-oxygen-dependent-citric-acid-production-by-yarrowia-lipolytica-insights-through-transcriptome-and-fluxome-analyses
#9
Wael Sabra, Rajesh Reddy Bommareddy, Garima Maheshwari, Seraphim Papanikolaou, An-Ping Zeng
BACKGROUND: Unlike the well-studied backer yeast where catabolite repression represents a burden for mixed substrate fermentation, Yarrowia lipolytica, an oleaginous yeast, is recognized for its potential to produce single cell oils and citric acid from different feedstocks. These versatilities of Y. lipolytica with regards to substrate utilization make it an attractive host for biorefinery application. However, to develop a commercial process for the production of citric acid by Y. lipolytica, it is necessary to better understand the primary metabolism and its regulation, especially for growth on mixed substrate...
May 8, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28482838/beneficial-effects-on-host-energy-metabolism-of-short-chain-fatty-acids-and-vitamins-produced-by-commensal-and-probiotic-bacteria
#10
REVIEW
Jean Guy LeBlanc, Florian Chain, Rebeca Martín, Luis G Bermúdez-Humarán, Stéphanie Courau, Philippe Langella
The aim of this review is to summarize the effect in host energy metabolism of the production of B group vitamins and short chain fatty acids (SCFA) by commensal, food-grade and probiotic bacteria, which are also actors of the mammalian nutrition. The mechanisms of how these microbial end products, produced by these bacterial strains, act on energy metabolism will be discussed. We will show that these vitamins and SCFA producing bacteria could be used as tools to recover energy intakes by either optimizing ATP production from foods or by the fermentation of certain fibers in the gastrointestinal tract (GIT)...
May 8, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28468662/heterologous-expression-of-abaecin-peptide-from-apis-mellifera-in-pichia-pastoris
#11
Denis Prudencio Luiz, Juliana Franco Almeida, Luiz Ricardo Goulart, Nilson Nicolau-Junior, Carlos Ueira-Vieira
BACKGROUND: Antimicrobial peptides (AMPs) are the first line of host immune defense against pathogens. Among AMPs from the honeybee Apis mellifera, abaecin is a major broad-spectrum antibacterial proline-enriched cationic peptide. RESULTS: For heterologous expression of abaecin in Pichia pastoris, we designed an ORF with HisTag, and the codon usage was optimized. The gene was chemically synthetized and cloned in the pUC57 vector. The new ORF was sub-cloned in the pPIC9 expression vector and transformed into P...
May 3, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28468624/direct-hydrogen-production-from-dilute-acid-pretreated-sugarcane-bagasse-hydrolysate-using-the-newly-isolated-thermoanaerobacterium-thermosaccharolyticum-mj1
#12
Bin-Bin Hu, Ming-Jun Zhu
BACKGROUND: Energy shortage and environmental pollution are two severe global problems, and biological hydrogen production from lignocellulose shows great potential as a promising alternative biofuel to replace the fossil fuels. Currently, most studies on hydrogen production from lignocellulose concentrate on cellulolytic microbe, pretreatment method, process optimization and development of new raw materials. Due to no effective approaches to relieve the inhibiting effect of inhibitors, the acid pretreated lignocellulose hydrolysate was directly discarded and caused environmental problems, suggesting that isolation of inhibitor-tolerant strains may facilitate the utilization of acid pretreated lignocellulose hydrolysate...
May 3, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28464872/functional-screening-of-aldehyde-decarbonylases-for-long-chain-alkane-production-by-saccharomyces-cerevisiae
#13
Min-Kyoung Kang, Yongjin J Zhou, Nicolaas A Buijs, Jens Nielsen
BACKGROUND: Low catalytic activities of pathway enzymes are often a limitation when using microbial based chemical production. Recent studies indicated that the enzyme activity of aldehyde decarbonylase (AD) is a critical bottleneck for alkane biosynthesis in Saccharomyces cerevisiae. We therefore performed functional screening to identify efficient ADs that can improve alkane production by S. cerevisiae. RESULTS: A comparative study of ADs originated from a plant, insects, and cyanobacteria were conducted in S...
May 2, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28464820/purification-and-characterization-of-a-novel-cold-adapted-fungal-glucoamylase
#14
Mario Carrasco, Jennifer Alcaíno, Víctor Cifuentes, Marcelo Baeza
BACKGROUND: Amylases are used in various industrial processes and a key requirement for the efficiency of these processes is the use of enzymes with high catalytic activity at ambient temperature. Unfortunately, most amylases isolated from bacteria and filamentous fungi have optimal activity above 45 °C and low pH. For example, the most commonly used industrial glucoamylases, a type of amylase that degrades starch to glucose, are produced by Aspergillus strains displaying optimal activities at 45-60 °C...
May 2, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28454584/direct-expression-of-active-human-tissue-inhibitors-of-metalloproteinases-by-periplasmic-secretion-in-escherichia-coli
#15
Ki Baek Lee, Dong Hyun Nam, Jacob A M Nuhn, Juan Wang, Ian C Schneider, Xin Ge
BACKGROUND: As regulators of multifunctional metalloproteinases including MMP, ADAM and ADAMTS families, tissue inhibitors of metalloproteinases (TIMPs) play a pivotal role in extracellular matrix remodeling, which is involved in a wide variety of physiological processes. Since abnormal metalloproteinase activities are related to numerous diseases such as arthritis, cancer, atherosclerosis, and neurological disorders, TIMPs and their engineered mutants hold therapeutic potential and thus have been extensively studied...
April 28, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28446197/re-directing-bacterial-microcompartment-systems-to-enhance-recombinant-expression-of-lysis-protein-e-from-bacteriophage-%C3%AF-x174-in-escherichia-coli
#16
Mimi C Yung, Feliza A Bourguet, Timothy S Carpenter, Matthew A Coleman
BACKGROUND: Recombinant expression of toxic proteins remains a challenging problem. One potential method to shield toxicity and thus improve expression of these proteins is to encapsulate them within protein compartments to sequester them away from their targets. Many bacteria naturally produce so-called bacterial microcompartments (BMCs) in which enzymes comprising a biosynthetic pathway are encapsulated in a proteinaeous shell, which is in part thought to shield the cells from the toxicity of reaction intermediates...
April 26, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28446175/bacillus-pumilus-katx2-confers-enhanced-hydrogen-peroxide-resistance-to-a-bacillus-subtilis-pkata-katx2-mutant-strain
#17
Stefan Handtke, Dirk Albrecht, Daniela Zühlke, Andreas Otto, Dörte Becher, Thomas Schweder, Kathrin Riedel, Michael Hecker, Birgit Voigt
BACKGROUND: Bacillus pumilus cells exhibit a significantly higher resistance to hydrogen peroxide compared to closely related Bacilli like Bacillus subtilis. RESULTS: In this study we analyzed features of the catalase KatX2 of B. pumilus as one of the most important parts of the cellular response to hydrogen peroxide. KatX2, the vegetative catalase expressed in B. pumilus, was compared to the vegetative catalase KatA of B. subtilis. Data of our study demonstrate that B...
April 26, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28438207/large-scale-validation-of-an-efficient-crispr-cas-based-multi-gene-editing-protocol-in-escherichia-coli
#18
Francesca Zerbini, Ilaria Zanella, Davide Fraccascia, Enrico König, Carmela Irene, Luca F Frattini, Michele Tomasi, Laura Fantappiè, Luisa Ganfini, Elena Caproni, Matteo Parri, Alberto Grandi, Guido Grandi
BACKGROUND: The exploitation of the CRISPR/Cas9 machinery coupled to lambda (λ) recombinase-mediated homologous recombination (recombineering) is becoming the method of choice for genome editing in E. coli. First proposed by Jiang and co-workers, the strategy has been subsequently fine-tuned by several authors who demonstrated, by using few selected loci, that the efficiency of mutagenesis (number of mutant colonies over total number of colonies analyzed) can be extremely high (up to 100%)...
April 24, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28438205/expression-of-the-clonostachys-rosea-lactonohydrolase-gene-by-lactobacillus-reuteri-to-increase-its-zearalenone-removing-ability
#19
Wen-Chun Yang, Tsui-Chun Hsu, Kuan-Chen Cheng, Je-Ruei Liu
BACKGROUND: Mycotoxins are secondary metabolites produced by filamentous fungi that can contaminate agricultural crops in the field as well as during harvest, transportation, processing, or storage. Zearalenone (ZEN), a non-steroidal estrogenic mycotoxin, produced by Fusarium species, has been shown to be associated with reproductive disorders in farm animals and to a lesser extent in hyperoestrogenic syndromes in humans. Thus, the decontamination of ZEN in foods and feeds is an important issue...
April 24, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28438200/a-novel-strategy-to-improve-protein-secretion-via-overexpression-of-the-sppa-signal-peptide-peptidase-in-bacillus-licheniformis
#20
Dongbo Cai, Hao Wang, Penghui He, Chengjun Zhu, Qin Wang, Xuetuan Wei, Christopher T Nomura, Shouwen Chen
BACKGROUND: Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a central component for extracellular protein production, there has been a lack of research on whether protein secretion could be enhanced via overexpression of signal peptide peptidases. RESULTS: In this study, both nattokinase and α-amylase were employed as prototypical secreted target proteins to evaluate the function of putative signal peptide peptidases (SppA and TepA) in Bacillus licheniformis...
April 24, 2017: Microbial Cell Factories
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