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Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

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https://www.readbyqxmd.com/read/28917128/application-of-ultra-high-performance-liquid-chromatography-coupled-with-ltq-orbitrap-mass-spectrometry-for-identification-confirmation-and-quantitation-of-illegal-adulterated-weight-loss-drugs-in-plant-dietary-supplements
#1
Qiaoyuan Cheng, Linjun Shou, Cen Chen, Shi Shi, Minghao Zhou
In this paper, an ultra-high-performance liquid chromatography coupled with linear ion trap quadrupole Orbitrap high resolution mass spectrometry (UHPLC-LTQ-Orbitrap HRMS) method was developed and validated for identification, confirmation and quantitation of illegal adulterated weight-loss drugs in plant dietary supplements. 13 wt-loss drugs were well separated by the gradient elution of 10mmol/L ammonium acetate - 0.05% formic acid H2O and acetonitrile at a flow rate of 0.2mL/min within 12min. The MS analysis was operated under the positive ion and in full MS/dd-MS(2) (data-dependent MS(2)) mode...
September 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28915421/determination-of-chlormequat-and-mepiquat-residues-and-their-dissipation-rates-in-tomato-cultivation-matrices-by-ultra-performance-liquid-chromatography-tandem-mass-spectrometry
#2
Hongxia Zhang, Zheng Feei Ma, Haiyan Yang, Lingming Kong
This study described the development and validation of a simple, rapid, specific and sensitive method for detecting chlormequat chloride (CQ) and mepiquat chloride (MQ) residues in tomato cultivation matrices covering soil, water, seedling samples. The dissipation rates of CQ and MQ in tomato cultivation matrices were also determined in this study. A Hydrophilic Interaction Liquid Chromatography (HILIC) column was used for chromatographic separation. A triple quadrupole mass spectrometer equipped with an electrospray ionisation source in positive ion mode by multiple reaction monitoring was used for detection...
September 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28915420/determination-of-eight-quinolones-in-milk-using-immunoaffinity-microextraction-in-a-packed-syringe-and-liquid-chromatography-with-fluorescence-detection
#3
Xinda Zhang, Cuicui Wang, Linyan Yang, Wei Zhang, Jing Lin, Cun Li
We have established a new, highly selective, and sensitive method for the determination of eight quinolones (QNs) in milk: danofloxacin, enrofloxacin, orbifloxacin, norfloxacin, ofloxacin, lomefloxacin, fleroxacin, and ciprofloxacin. The method uses immunoaffinity microextraction in a packed syringe and liquid chromatography with fluorescence detection (IA-MEPS-LC-FLD). Traditionally, QN residues are determined by liquid-liquid extraction (LLE) and solid phase extraction (SPE) sample preparation techniques; however, these methods are time-consuming and require large quantities of organic solvents...
September 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28915419/sensitivity-improvement-of-the-lc-ms-ms-quantification-of-carbidopa-in-human-plasma-and-urine-by-derivatization-with-2-4-pentanedione
#4
Nico C van de Merbel, Kees J Bronsema, Steven H Gorman, Ray Bakhtiar
The reliable quantification of carbidopa in biological samples at low concentrations is challenging because of the polar and highly unstable nature of the compound. In this paper, LC-MS/MS methods are described for the determination of carbidopa in 50μL of human plasma and 25μL of human urine in the concentration ranges 1-1,000ng/mL and 100-50,000ng/mL, respectively. After a simple protein precipitation (plasma) or dilution (urine) step, carbidopa is derivatized at its hydrazine moiety by reaction for one hour with 2,4-pentanedione under acidic conditions and at 40°C...
September 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28922649/determination-of-74-new-psychoactive-substances-in-serum-using-automated-in-line-solid-phase-extraction-liquid-chromatography-tandem-mass-spectrometry
#5
Sabrina Lehmann, Tobias Kieliba, Justus Beike, Mario Thevis, Katja Mercer-Chalmers-Bender
A detailed description is given of the development and validation of a fully automated in-line solid-phase extraction-liquid chromatography-tandem mass spectrometry (SPE-LC-MS/MS) method capable of detecting 90 central-stimulating new psychoactive substances (NPS) and 5 conventional amphetamine-type stimulants (amphetamine, 3,4-methylenedioxy-methamphetamine (MDMA), 3,4-methylenedioxy-amphetamine (MDA), 3,4-methylenedioxy-N-ethyl-amphetamine (MDEA), methamphetamine) in serum. The aim was to apply the validated method to forensic samples...
September 7, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28915422/determination-of-hydroxyurea-in-human-plasma-by-hplc-uv-using-derivatization-with-xanthydrol
#6
Tiphaine Legrand, Marie-Georgine Rakotoson, Frédéric Galactéros, Pablo Bartolucci, Anne Hulin
A simple and rapid high performance liquid chromatography (HPLC) method using ultraviolet (UV) detection was developed to determine hydroxyurea (HU) concentration in plasma sample after derivatization with xanthydrol. Two hundred microliters samples were spiked with methylurea (MeU) as internal standard and proteins were precipitated by adding methanol. Derivatization of HU and MeU was immediately performed by adding 0.02M xanthydrol and 1.5M HCl in order to obtain xanthyl-derivatives of HU and MeU that can be further separated using HPLC and quantified using UV detection at 240nm...
September 7, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28890363/relationship-between-the-color-stability-and-impurity-profile-of-cefotaxime-sodium
#7
Hua Sun, Xuejun Cui, Baoshu Liu, Junli Zhang
The color grade, mainly introduced in the processes of semisynthesis and storage, is an important index used to evaluate the quality of cefotaxime sodium. Because the drug itself is prone to degradation under susceptible conditions, including those involving moisture, heat, ultraviolet light, acids, alkalis, and oxidants, and a series of degradation products as impurities are generated. In this study, the factors affecting color grade stability and the degradation mechanisms of cefotaxime sodium were investigated by designing different accelerated stability tests under the aforementioned conditions...
September 7, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28910661/describing-the-holistic-toxicokinetics-of-hepatotoxic-chinese-herbal-medicines-by-a-novel-integrated-strategy-dioscorea-bulbifera-rhizome-as-a-case-study
#8
Ling-Li Wang, Dong-Sheng Zhao, Wei Shi, Zhuo-Qing Li, Zi-Tian Wu, Ping Li, Hui-Jun Li
It is vital to monitor the holistic toxicokinetics of toxic Chinese herbal medicines (CHMs) for safety. Although an integrated strategy based on the area under the curve (AUC) has been proposed to characterize the pharmacokinetic/toxicokinetic properties of CHMs, improvement is still needed. This study attempted to use 50% inhibitory concentration (IC50) as weighting coefficient to investigate holistic toxicokinetics of the major diosbulbins i.e. diosbulbin A (DA), diosbulbin B (DB), and diosbulbin C (DC) after oral administration of Dioscorea bulbifera rhizome (DBR) extract...
September 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28918318/high-sensitivity-method-validated-to-quantify-estradiol-in-human-plasma-by-lc-ms-ms
#9
Mônica Siqueira Ferreira, André M M Arruda, Giovanni Tieghi Pepi, Aline Cristina Martho, Pâmela Maria Maximiano, Lina S O B O Ricci, Maria Francesca Riccio, Ana Cláudia Noboli, Pedro Serafim
17β-Estradiol (E2) is an endogenous steroid in the human body. Its measurement is important for health and human biology understanding. However, E2 concentration in human plasma is in the range of pg/mL, which makes it difficult to detect. In this way, LC-MS/MS has been shown the most sensitive tool, although E2 is a weakly ionizable molecule. In this work, we validated a more sensitive and accurate method for E2 quantification in human plasma. Our extraction step ensured a cleaner chromatography, resulting in a precise measurement and highly reproducible method in the range of 2-150pg/mL...
September 5, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28915418/purification-identification-and-molecular-mechanism-of-two-dipeptidyl-peptidase-iv-dpp-iv-inhibitory-peptides-from-antarctic-krill-euphausia-superba-protein-hydrolysate
#10
Wei Ji, Chaohua Zhang, Hongwu Ji
Dipeptidyl peptidase IV (DPP-IV) played an important role in blood glucose regulation. Inhibition of DPP-IV may improve glycemic control in diabetics by preventing the rapid breakdown of incretin hormones and prolonging their physiological action. In this study, Antarctic krill (Euphausia superba) protein was hydrolyzed using animal proteolytic enzymes. The hydrolysate was purified sequentially by ultrafiltration, gel filtration chromatography and reversed phase high-performance liquid chromatography (RP-HPLC)...
September 5, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28915417/lc-ms-ms-quantification-of-7%C3%AE-hydroxy-4-cholesten-3-one-c4-in-rat-and-monkey-plasma
#11
Lijuan Kang, Thomas M Connolly, Naidong Weng, Wenying Jian
7α-hydroxy-4-cholesten-3-one (C4) is an oxidative enzymatic product of cholesterol metabolism via cholesterol 7α-hydroxylase, an enzyme also known as cholesterol 7-alpha-monooxygenase or cytochrome P450 7A1 (CYP7A1). C4 is a stable intermediate in the rate limiting pathway of bile acid biosynthesis. Previous studies showed that plasma C4 levels correlated with CYP7A1 enzymatic activity and could serve as a biomarker for bile acid synthesis. Here we developed and qualified a simple and robust high-throughput method using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) to quantify C4 in rat and monkey plasma...
September 5, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28898719/a-cost-effective-lc-ms-ms-method-for-identification-and-quantification-of-%C3%AE-amanitin-in-rat-plasma-application-to-toxicokinetic-study
#12
Chunlei Li, Fen Wei, Saqib Muhammad, Guangde Yang, Sicen Wang, Xinshe Liu
α-Amanitin is the main lethal component of amanita mushrooms, and data on its toxicokinetics are few. The aim of this study was to develop a sensitive and cost-effective method to identify α-amanitin and investigate its toxicokinetic parameters using liquid chromatography-triple quadrupole tandem mass spectrometry. The colchicine was used as the internal standard (IS). The compounds were extracted from plasma samples by protein precipitation with acetonitrile (containing 1% formic acid). The analysis was performed through multiple reactions monitoring...
September 5, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28892742/toxicity-teratogenicity-and-antibacterial-activity-of-posterior-salivary-gland-psg-toxin-from-the-cuttlefish-sepia-pharaonis-ehrenberg-1831
#13
Ramachandran Karthik, Venkatesan Manigandan, Ramachandran Saravanan
Toxins from the posterior salivary gland (PSG) of cuttlefishes are known toxins with pronounced toxicity. In the present study, ionic peptide rich PSG toxin from the cuttlefish S. pharaonis was isolated by ion exchange chromatography and purified by Reversed Phase High Performance Liquid Chromatography (RP-HPLC), with active fraction at a retention time of 26min. The net protein content of the PSG toxin was estimated to be 46.6mg at a proximate molecular weight of∼50kDa. Fourier Transform Infrared Spectroscopy (FT-IR) of PSG toxin revealed the presence of alcoholic OH, primary NH, alkyl CH and conjugated CONH functional groups...
September 5, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28918319/determination-of-chlorogenic-acids-and-caffeine-in-homemade-brewed-coffee-prepared-under-various-conditions
#14
Jong-Sup Jeon, Han-Taek Kim, Il-Hyung Jeong, Se-Ra Hong, Moon-Seog Oh, Kwang-Hee Park, Jae-Han Shim, A M Abd El-Aty
Coffee, a complex mixture of more than 800 volatile compounds, is one of the most valuable commodity in the world, whereas caffeine and chlorogenic acids (CGAs) are the most common compounds. CGAs are mainly composed of caffeoylquinic acids (CQAs), dicaffeoylquinic acids (diCQAs), and feruloylquinic acids (FQAs). The major CGAs in coffee are neochlorogenic acid (3-CQA), cryptochlorogenic acid (4-CQA), and chlorogenic acid (5-CQA). Many studies have shown that it is possible to separate the isomers of FQAs by high-performance liquid chromatography (HPLC)...
August 31, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28918317/bithionol-residue-analysis-in-animal-derived-food-products-by-an-effective-and-rugged-extraction-method-coupled-with-liquid-chromatography-tandem-mass-spectrometry
#15
Weijia Zheng, Jin-A Park, A M Abd El-Aty, Seong-Kwan Kim, Sang-Hyun Cho, Jeong-Min Choi, Hee Yi, Soo-Min Cho, H A El-Banna, Jae-Han Shim, Byung-Joon Chang, Jing Wang, Jin-Suk Kim, Ho-Chul Shin
Herein, we developed a simple analytical procedure for the quantitation of bithionol residues in animal-derived food products such as porcine muscle, eggs, milk, eel, flatfish, and shrimp using a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method coupled with liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI(+)/MS-MS). Samples were extracted with 0.1% solution of formic acid in acetonitrile and the extract was purified using a C18 sorbent. Separation was performed on a Waters XBridge™ C18 reversed-phase analytical column using 0...
August 31, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28886478/chaihu-shu-gan-san-regulates-phospholipids-and-bile-acid-metabolism-against-hepatic-injury-induced-by-chronic-unpredictable-stress-in-rat
#16
Hong-Mei Jia, Meng Yu, Li-Yan Ma, Hong-Wu Zhang, Zhong-Mei Zou
Chaihu-Shu-Gan-San (CSGS) is a famous classic traditional Chinese medicines (TCM) formula for treatment of liver stagnancy recorded in a famous book of traditional Chinese medicine, Jing Yue Quan Shu published in 1624. It has been extensively accepted as an antidepressant in China and its mechanism of action is still not clear. Previously we have found that hepatic injury happens in chronic unpredicted mild stress (CUMS). Thus, the protection of CSGS against hepatic injury induced by CUMS treatment was explored by metabonomics study and gene expression of the rat liver tissue...
August 31, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28881295/simultaneous-quantitation-of-hydrazine-and-acetylhydrazine-in-human-plasma-by-high-performance-liquid-chromatography-tandem-mass-spectrometry-after-derivatization-with-p-tolualdehyde
#17
Lu Song, Dan Gao, Shangfu Li, Yanwei Wang, Hongxia Liu, Yuyang Jiang
A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for simultaneous quantitative analysis of hydrazine and acetylhydrazine in human plasma based on the strategy of p-tolualdehyde derivatization. The derivatization reactions were easily realized by ultrasonic manipulation for 40min. Good separation of the derivatization products was achieved using a C18 column by gradient elution. The optimized mass transition ion-pairs (m/z) monitored for the two hydrazine derivatives were m/z 237...
August 30, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28869873/hplc-ms-ms-method-for-bioavailability-study-of-bruceines-d-e-in-rat-plasma
#18
Farahdina Man, Chee-Yan Choo
Bruceines D and E are quassinoids from seeds of Brucea javanica (L.) Merr. exhibiting hypoglycemia effect. The crude drug is used as a traditional medicine by diabetes patients. The aim of this study is to understand the bioavailability and pharmacokinetics of both the bruceines D & E. A rapid and sensitive HPLC-MS/MS method was developed and validated for the quantification of both quassinoids, bruceines D & E in rat plasma. Both the bruceines D & E were separated with the Zorbax SBC-18 column with gradient elution and mobile phase system of acetonitrile and deionized water with 0...
August 30, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28869872/semi-preparative-high-performance-countercurrent-chromatography-method-for-the-purification-of-chemically-synthesized-atp-analogue-apppi
#19
Elina Puljula, Petri A Turhanen
An efficient high-performance countercurrent chromatography (HPCCC) based method has been developed for the purification of chemically synthesized 1-adenosin-5'-yl 3-(3-methylbut-3-enyl)triphosphoric acid diester (ApppI). ApppI is an adenosine triphosphate (ATP) analogue with biological significance due to its varied actions in the body. ApppI was synthesized and purified as its tetrabutylammonium (TBA) salt and converted successfully into its more practical sodium salt form after purification. The amount of TBA hydroxide (2...
August 30, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28865332/metabolomic-study-for-monitoring-of-biomarkers-in-mouse-plasma-with-asthma-by-gas-chromatography-mass-spectrometry
#20
Chan Seo, Yun-Ho Hwang, Hyeon-Seong Lee, Youngbae Kim, Tae Hwan Shin, Gwang Lee, Young-Jin Son, Hangun Kim, Sung-Tae Yee, Ae Kyung Park, Man-Jeong Paik
Asthma is a multifaceted chronic disease caused by an alteration of various genetic and environmental factors that is increasing in incidence worldwide. However, the biochemical mechanisms regarding asthma are not completely understood. Thus, we performed of metabolomic study for understanding of the biochemical events by monitoring of altered metabolism and biomarkers in asthma. In mice plasma, 27 amino acids(AAs), 24 fatty acids(FAs) and 17 organic acids(OAs) were determined by ethoxycarbonyl(EOC)/methoxime(MO)/tert-butyldimethylsilyl(TBDMS) derivatives with GC-MS...
August 30, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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