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Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

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https://www.readbyqxmd.com/read/28806686/simultaneous-determination-of-16-macrolide-antibiotics-and-4-metabolites-in-milk-by-using-quick-easy-cheap-effective-rugged-and-safe-extraction-quechers-and-high-performance-liquid-chromatography-tandem-mass-spectrometry
#1
Weie Zhou, Yun Ling, Tong Liu, Yuan Zhang, Jianzhi Li, Hongna Li, Wenjie Wu, Shoujun Jiang, Feng Feng, Fei Yuan, Feng Zhang
A novel analytical method was developed and validated for simultaneous analysis of 16 macrolide antibiotics and 4 metabolites in milk. A modified quick, easy, cheap, effective, rugged, and safe extraction method (QuEChERS) optimized by response surface methodology (RSM) was used for sample preparation. All the drugs were subsequently separated and detected by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS), using roxithromycin as internal standards for maximum accuracy and precision...
August 5, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28800540/rapid-extraction-of-amomum-tsao-ko-essential-oil-and-determination-of-its-chemical-composition-antioxidant-and-antimicrobial-activities
#2
Qi Cui, Li-Tao Wang, Ju-Zhao Liu, Hui-Mei Wang, Na Guo, Cheng-Bo Gu, Yu-Jie Fu
A simple, green and efficient extraction method named modified-solvent free microwave extraction (M-SFME) was employed for the extraction of essential oils (EOs) from Amomun tsao-ko. The process of M-SFME was optimized with the prominent preponderance of such higher extraction yield (1.13%) than those of solvent free microwave extraction (SFME, 0.91%) and hydrodistillation (HD, 0.84%) under the optimal parameters. Thirty-four volatile substances representing 95.4% were identified. The IC50 values of EOs determined by DPPH radical scavenging activity and β-carotene/linoleic acid bleaching assay were 5...
August 3, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28803116/efficient-molecularly-imprinted-polymer-as-a-pipette-tip-solid-phase-sorbent-for-determination-of-carvedilol-enantiomers-in-human-urine
#3
Anny Talita Maria da Silva, Hanna Leijoto de Oliveira, Camilla Fonseca Silva, Matheus Cecílio Fonseca, Thales Fernando Dias Pereira, Clebio Soares Nascimento, Eduardo Costa de Figueiredo, Keyller Bastos Borges
In this work, an efficient pipette tip based on molecularly imprinted polymers solid-phase extraction (PT-MIP-SPE) method was developed for carvedilol (CAR) analysis. This compound is available in clinical practice as a racemic mixture, in which (-)-(S)-CAR is a β- and α1-adrenergic antagonist, while (+)-(R)-CAR only acts as an α1-adrenergic antagonist. Enantioseparation of CAR presented satisfactory retention times (5.85 and 14.84min), acceptable theoretical plates (N=2048 and 2018) and good resolution (Rs=9...
August 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28802218/pharmaceutical-and-biomedical-applications-of-dispersive-liquid-liquid-microextraction
#4
REVIEW
Fotouh R Mansour, Mostafa A Khairy
Sample treatment is so crucial in pharmaceutical and biomedical analysis. Proper sample preparation protects the analytical instrument, increases the sensitivity, and enhances the selectivity by removing probable interfering substances. Dispersive liquid-liquid microextraction (DLLME) is one of the most important approaches in sample treatment. In normal DLLME, the organic droplet of the extractant is mixed with a dispersing solvent before being injected into the sample. After manual or mechanical shaking, the cloudy solution is centrifuged to break the formed emulsion...
August 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28800539/development-of-a-rapid-and-sensitive-multiple-reaction-monitoring-proteomic-approach-for-quantification-of-transporters-in-human-liver-tissue
#5
Li Wang, Kasi J Rubadue, Jeffrey Alberts, David W Bedwell, Kenneth J Ruterbories
With increasing knowledge on the role of hepatic transporters in drug disposition, numerous efforts have been described to quantify the expression of human hepatic transporters. However, reported quantitative proteomic approaches often require long analysis times. Additionally, greater assay sensitivity is still necessary for less abundant transporters or limited quantity of samples (e.g. hepatocytes and liver tissue). In the present study, an LC-MS/MS method for rapid and simultaneous quantification of 12 hepatic transporters (BCRP, BSEP, MATE1, MRP2, MRP3, MRP4, NTCP, OATP1B1, 1B3, 2B1, OCT1, and P-gp) was developed...
August 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28800538/characterization-of-the-metabolic-transformation-of-thiamethoxam-to-clothianidin-in-helicoverpa-armigera-larvae-by-spe-combined-uplc-ms-ms-and-its-relationship-with-the-toxicity-of-thiamethoxam-to-helicoverpa-armigera-larvae
#6
Yinjun Fan, Xueyan Shi
In order to characterize the metabolic transformation of thiamethoxam (TMX) to clothianidin (CLO) in Helicoverpa armigera larvae and clarify its relationship with the insecticidal toxicity of TMX, method for determination of TMX and its metabolite clothianidin (CLO) residues in H. armigera larvae by solid phase extraction (SPE) combined UPLC-MS/MS was established. Following acetonitrile extraction and purification by SPE on florisil cartridge and C18 cartridge sequently, and cleanup by PSA adsorption, TMX and CLO residues in H...
July 31, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28772224/nanofibers-mat-based-solid-phase-extraction-method-for-the-pretreatment-of-urine-samples-and-its-application-in-the-primary-study-on-the-disposition-of-nonylphenol-after-long-term-low-level-exposure-in-rats
#7
Liangliang Qian, Ruixian Li, Qiannan Di, Yang Shen, Qian Xu, Jian Li
A method was established for the analysis of nonylphenol (NP) in rat urine samples based on a solid-phase extraction (SPE) procedure with an amino functionalized polyacrylonitrile nanofibers mat (NH2-PAN NFSM) as sorbent coupled with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The calibration curves prepared in three different days showed good linearity over a wide range of NP concentrations from 0.1 to 100.0ng/mL. It was remarkable that the proposed NH2-PAN NFsM based SPE method showed superior extraction efficiency with the consumption of only 4mg of sorbent and 500μL of eluent...
July 31, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28802217/comparatively-evaluating-the-pharmacokinetic-of-fifteen-constituents-in-normal-and-blood-deficiency-rats-after-oral-administration-of-xin-sheng-hua-granule-by-uplc-ms-ms
#8
Han-Qing Pang, Yu-Ping Tang, Yu-Jie Cao, Ya-Jie Tan, Yi Jin, Xu-Qin Shi, Sheng-Liang Huang, Da-Zheng Sun, Jin Sun, Zhi-Shu Tang, Jin-Ao Duan
Xin-Sheng-Hua Granule (XSHG), a famous traditional Chinese medicine prescription, are clinically applied for the treatment of postpartum disease through nourishing blood and promoting blood circulation. In this investigation, a multi-constituents (trigonelline, stachydrine hydrochloride, hydroxysafflor yellow A, chlorogenic acid, amygdalin, leonurine, liquiritin, ferulic acid, senkyunolide I, senkyunolide H, glycyrrhizic acid, senkyunolide A, ligustilide, butylidenephthalide and glycyrrhetinic acid) pharmacokinetic study of XSHG was conducted for the first time...
July 30, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28787651/proteome-analysis-of-vitreous-humor-in-retinal-detachment-using-two-different-flow-charts-for-protein-fractionation
#9
Leonor M Gaspar, Fátima M Santos, Tânia Albuquerque, João P Castro-de-Sousa, Luís A Passarinha, Cândida T Tomaz
The deeper understanding of retinal detachment (RD) pathogenesis may improve the visual outcome after surgery. Given the main role of the vitreous in retinal eye diseases, two strategies were explored to identify its proteome in RD. Fractionation techniques such as anion exchange chromatography (IEX) and SDS-PAGE combined with MALDI-TOF/TOF analysis allowed to identify 127 proteins in vitreous of RD patients. From these proteins, 19 were identified using only the IEX fractionation strategy, and 117 using a bidimensional (IEX and SDS-PAGE) fractionation...
July 29, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28783562/simultaneous-determination-of-paeonilactone-a-and-paeonilactone-b-in-rat-plasma-after-oral-administration-of-albiflorin-by-uplc-tof-ms-following-picolinoyl-derivatization
#10
Zhigang Wang, Shuhan Tang, Masao Hattori, Hailong Zhang, Xiuhong Wu
A new highly sensitive analytical method was developed to investigate the in vivo metabolism of albiflorin, one of the most principal components in traditional Chinese medicine. After hydrolyzation with sulfatase, the main metabolites paeonilactone A and paeonilactone B of paeoniflorin in rat plasma were successfully detected for the first time by liquid chromatography mass spectrometry following picolinoyl derivatization. Borneol was used as the internal standard compound to quantify paeonilactone A and paeonilactone B in rat plasma...
July 29, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28778038/application-of-magnetically-immobilized-edible-fungus-for-the-biotransformation-of-panax-notoginseng-saponin-rb1-to-rd-and-rg3
#11
Yuping Zang, Na Guo, Jiao Jiao, Xiqing Wang, Qingyan Gai, Wenjing Xu, Yujie Fu
In this study, we developed a new magnetically immobilized edible fungus for the biotransformation of panax notoginseng saponins Rb1 to Rd and Rg3. The optimum biotransformation conditions were as follows: temperature 32°C, pH 6.5, time 48h and liquid-solid ratio 25:1(mL/g). The yields of Rd and Rg3 reached 41.35±0.12mg/g and 6.35±0.08mg/g which increased 6.97-fold and 3.23-fold to that of untreated control, respectively. Additionally, SEM demonstrated that vestured pits and cell walls of samples were destroyed obviously which was beneficial to the Rb1 biotransformation and target Rd and Rg3 release...
July 27, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28763758/simultaneous-determination-of-three-di-caffeoylquinic-acids-by-uhplc-ms-ms-in-rat-plasma-and-its-application-to-a-comparative-pharmacokinetic-study-in-normal-and-acute-lung-injury-rat
#12
Yanjuan Wang, Yanmin Wang, Xiao Wu, Zhili Xiong, Wei Xiao, Chao Ma
Acute lung injury (ALI) is a severe inflammatory disease with high mortality rates. Di-caffeoylquinic acids (DCQAs), the bioactive components of reduning injection (RDN), may play important roles in the protective effect on acute lung injury (ALI). A selective and rapid ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method has been developed and validated for the simultaneous determination of 3,4-, 3,5- and 4,5-DCQA in rat plasma. The DCQAs were extracted by liquid-liquid extraction with ethyl acetate-isopropyl alcohol (7:3, v/v)...
July 25, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28772225/an-lc-ms-ms-method-for-quantitation-of-chemopreventive-sphingadienes-in-food-products-and-biological-samples
#13
J H Suh, A M Makarova, J M Gomez, L A Paul, J D Saba
Colorectal cancer (CRC) is a leading cause of cancer mortality. Diet has a significant influence on colon cancer risk. Identifying chemopreventive agents, dietary constituents, practices and/or diet supplements that promote gut health and reduce the incidence of intestinal neoplasias and CRC could significantly impact public health. Sphingadienes (SDs) are dietary sphingolipids found in plant-based food products. SDs are cytotoxic to colon cancer cells and exhibit chemopreventive properties. The aim of the present study was to develop a sensitive and robust ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for quantifying SDs in food products and biological samples...
July 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28763759/metabolomics-analysis-of-danggui-sini-decoction-on-treatment-of-collagen-induced-arthritis-in-rats
#14
Bang Cheng, Hua Zheng, Fang Wu, Jinxia Wu, Xuwen Liu, Chaoling Tang, Shiyin Lu, Zhaoni Chen, Fangming Song, Junxiang Ruan, Hongye Zhang, Hui Song, Wei Liu, Hongwei Guo, Yonghong Liang, Zhiheng Su
Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by persistent joint inflammation leading to bone and cartilage damage and even disability. However, the pathogenesis of RA is multi-factorial and to a large degree, remains unknown. Danggui Sini decoction (DSD), a traditional Chinese medicine (TCM) formula, has been widely used as a remedy for rheumatoid arthritis (RA) in recent years. In our study, (1)H-nuclear magnetic resonance ((1)H NMR) based metabolomics analysis of 7 potential biomarkers, including taurine (1), urea (2), betaine (3), pyruvate (4), hippurate (5), succinate (6) and acetone (7) was performed to investigate the progression of RA and assess the efficacy of DSD in collagen-induced arthritis (CIA) rats...
July 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28759841/a-fast-and-sensitive-lc-ms-ms-method-for-the-quantification-of-fosfomycin-in-human-urine-and-plasma-using-one-sample-preparation-method-and-hilic-chromatography
#15
Rixt A Wijma, Soma Bahmany, E B Wilms, T van Gelder, Johan W Mouton, Birgit C P Koch
Fosfomycin is an old antibiotic that is increasingly prescribed because of emergence of the antibiotic resistance and the growing incidence of multi-drug resistant infections. Surprisingly, little is known about its pharmacokinetics (PK) and the pharmacodynamics (PD). Quantification of fosfomycin in both urine and plasma provides insight into the PK/PD characteristics of fosfomycin, which is crucial for the optimization of the therapy and the prevention of the emergence of resistance. An analytical method is therefore needed for the quantification of fosfomycin in both urine and plasma...
July 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28778039/simultaneous-quantification-six-active-compounds-in-rat-plasma-by-uplc-ms-ms-and-its-application-to-a-pharmacokinetic-study-of-pien-tze-huang
#16
Wen Xu, Yiping Zhang, Caijie Zhou, Yanni Tai, Xiaoqing Zhang, Jie Liu, Mei Sha, Mingqing Huang, Yanlin Zhu, Jun Peng, Jin-Jian Lu
Pien-Tze-Huang (PZH) is a popular traditional Chinese medicine (TCM) formula in China, but its pharmacokinetics has not been investigated yet. To better study the pharmacokinetic behaviors of PZH, an optimal ultra-performance liquid chromatography with triple quadrupole mass spectrometry (UPLC-MS/MS) method was developed for rapid quantification of six compounds (notoginsenoside R1, ginsenosides Re, Rg1, Rb1, Rd, and muscone) in rat plasma after oral administration of PZH. All analytes were extracted by protein precipitation with acetonitrile and separated on a Waters Acquity Cortecs C18 column within 3...
July 23, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28772226/liquid-chromatography-tandem-mass-spectrometric-assay-for-the-quantitative-determination-of-the-tyrosine-kinase-inhibitor-quizartinib-in-mouse-plasma-using-salting-out-liquid-liquid-extraction
#17
Irene A Retmana, Jing Wang, Alfred H Schinkel, Jan H M Schellens, Jos H Beijnen, Rolf W Sparidans
A bioanalytical assay for quizartinib -a potent, and selective FLT3 tyrosine kinase inhibitor- in mouse plasma was developed and validated. Salting-out assisted liquid-liquid extraction (SALLE), using acetonitrile and magnesium sulfate, was selected as sample pretreatment with deuterated quizartinib as internal standard. Separation was performed with reversed-phase liquid chromatography followed by detection with positive electrospray-triple quadrupole mass spectrometry in the selected reaction monitoring mode...
July 23, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28756357/high-performance-liquid-chromatography-with-time-programmed-fluorescence-detection-for-the-quantification-of-levofloxacin-in-human-plasma-and-cerebrospinal-fluid-in-adults-with-tuberculous-meningitis
#18
Pham Van Toi, Thomas Pouplin, Nguyen Duc Khanh Tho, Pham Nguyen Phuong, Tran Thi Hong Chau, Nguyen Thuy Thuong Thuong, Dorothee Heemskerk, Tran Tinh Hien, Guy E Thwaites
An accurate and reliable high-performance liquid chromatography with time-programmed fluorescence detection was developed and validated to measure levofloxacin in human plasma and cerebrospinal fluid (CSF). After solid phase extraction process using Evolute(®) ABN 96 fixed well plate; levofloxacin and internal standard-enoxacin were separated using a mobile phase consisting of phosphate buffer 10mM with 0.025% triethylamine pH 3.0 - acetonitrile (88:12, v/v) on a Purosphere RP-8e column (5μm, 125×4.0mm) at a flow rate of 1...
July 20, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28753519/enantioselectivity-of-anteiso-fatty-acids-in-hitherto-uninspected-sample-matrices
#19
Dorothee Eibler, Carolin Seyfried, Walter Vetter
Anteiso-fatty acids (aFAs) are chiral molecules due to a methyl substituent on the antepenultimate carbon of the otherwise straight acyl chain. 12-Methyltetradecanoic acid (a15:0) and 14-methylhexadecanoic acid (a17:0) are the predominant aFAs in nature but their individual contributions e.g. to food lipids are usually low. Enantioselective data has been collected in fish, bovine milk/cheese, and Brussels sprouts. In this study, we determined the enantioselectivity of a15:0 and a17:0 in shea butter, moose and camel milk, two soil samples and mold (collected from contaminated cheese)...
July 20, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28780068/the-use-of-lc-predicted-retention-times-to-extend-metabolites-identification-with-swath-data-acquisition
#20
Tobias Bruderer, Emmanuel Varesio, Gérard Hopfgartner
The application of predicted LC retention time to support metabolite identification was evaluated for a metabolomics MS/MS database containing 532 compounds representative for the major human metabolite classes. LC retention times could be measured for two C18 type columns using a mobile phase of pH=3.0 for positive ESI mode (n=337, 228) and pH=8.0 for negative ESI mode (n=410, 233). A QSRR modelling was applied with a small set of model compound selected based on the Kennard-Stone algorithm. The models were implemented in the R environment and can be applied to any library...
July 19, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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