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Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

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https://www.readbyqxmd.com/read/29775840/development-of-a-lc-ms-method-for-simultaneous-determination-of-amoxicillin-and-metronidazole-in-human-serum-using-hydrophilic-interaction-chromatography-hilic
#1
Udani L Kathriarachchi, Sagar S Vidhate, Naser Al-Tannak, Alison H Thomson, Michael J J da Silva Neto, David G Watson
A method was developed for the determination of amoxicillin and metronidazole in human serum. The procedure used was hydrophilic interaction chromatography (HILIC) followed by mass spectrometric (MS) detection. Chromatographic separation was achieved on a ZIC-HILIC column and the mobile phase consisted of a mixture of 0.1% (v/v) formic acid in water and 0.1% (v/v) formic acid in acetonitrile. The method was validated with regard to selectivity, accuracy, precision, calibration, lower limit of quantification (LOQ), extraction recovery and matrix effect...
May 15, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29763745/analysis-of-malondialdehyde-in-human-plasma-samples-through-derivatization-with-2-4-dinitrophenylhydrazine-by-ultrasound-assisted-dispersive-liquid-liquid-microextraction-gc-fid-approach
#2
Reyhane Malaei, Amir M Ramezani, Ghodratollah Absalan
A sensitive and reliable ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) procedure was developed and validated for extraction and analysis of malondialdehyde (MDA) as an important lipids-peroxidation biomarker in human plasma. In this methodology, to achieve an applicable extraction procedure, the whole optimization processes were performed in human plasma. To convert MDA into readily extractable species, it was derivatized to hydrazone structure-base by 2,4-dinitrophenylhydrazine (DNPH) at 40 °C within 60 min...
May 4, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29753212/quantification-of-doping-compounds-in-faecal-samples-from-racing-pigeons-by-liquid-chromatography-tandem-mass-spectrometry
#3
Fernando X Moreira, Renata Silva, Maria B André, Paula G de Pinho, Maria L Bastos, João Ruivo, Patrícia Ruivo, Helena Carmo
The use of performance enhancing drugs is not only common in humans, but also in animal sports, including racing of horses, greyhounds and pigeons. The development of accurate analytical procedures to detect doping agents in sports is crucial in order to protect the fair-play of the game, avoid financial fraud in the attribution of eventual awards and, even more important, to protect the animals from harmful drugs and/or dangerous dosage regimens. The present study aimed to develop and validate, a method that enabled the screening and confirmation of the presence of a beta-agonist (clenbuterol) and three corticosteroids (betamethasone, prednisolone and budesonide) in faeces from pigeons...
May 4, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29758408/abundant-plasma-protein-depletion-using-ammonium-sulfate-precipitation-and-protein-a-affinity-chromatography
#4
Lentel Pringels, Valérie Broeckx, Kurt Boonen, Bart Landuyt, Liliane Schoofs
Plasma is a highly valuable resource for biomarker research since it is easy obtainable and contains a high amount of information on patient health status. Although advancements in the field of proteomics enabled analysis of the plasma proteome, identification of low abundant proteins remains challenging due to high complexity and large dynamic range. In order to reduce the dynamic range of protein concentrations, a tandem depletion technique consisting of ammonium sulfate precipitation and Protein A affinity chromatography was developed...
May 1, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29753211/lc-ms-ms-method-for-determination-of-cyclin-dependent-kinase-inhibitors-bp-14-and-bp-20-and-its-application-in-pharmacokinetic-study-in-rat
#5
Jitka Široká, Martina Čečková, Lubor Urbánek, Vladimír Kryštof, Tomáš Gucký, Jakub Hofman, Miroslav Strnad, František Štaud
N2 -(4-Amino-cyclohexyl)-9-cyclopentyl-N6 -(6-furan-2-yl-pyridine-3-ylmethyl)-9H-purine-2,6-diamine (BP-14) and 2-(5-{[2-(4-amino-cyclohexylamino)-9-cyclopentyl-9H-purine-6-ylamino]-methyl}-pyridine-2-yl)-phenol (BP-20) are novel cyclin-dependent kinase inhibitors, structurally related to roscovitine, with significant biological activity. A simple, selective and sensitive liquid chromatography - tandem mass spectrometry method for determining them in rat plasma, using roscovitine as an internal standard, was developed and validated...
May 1, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29738966/sulfotransferase-catalyzed-biotransformation-of-liguzinediol-and-comparison-of-its-metabolism-in-different-species-using-uflc-qtof-ms
#6
Fei Shen, Hong-Mei Wen, Chen-Xiao Shan, An Kang, Bang Dong, Chuan Chai, Ji-Yun Zhang, Qi Zhang, Wei Li
Liguzinediol (2,5-dihydroxymethyl-3,6-dimethylpyrazine, LZDO) is a potential agent for the low-risk treatment of heart failure. 2-N-acetylcysteine-LZDO (2-NAC-LZDO) and 2-cysteine-LZDO (2-Cys-LZDO) are major LZDO metabolites found in the pharmacokinetic studies of rats and beagle dogs. To elucidate the biotransformation pathway and related enzymes, an incubation system with 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as a cofactor and N-acetylcysteine (NAC) as a trapping agent was established using liver cytosol...
May 1, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29738963/purification-of-r-phycoerythrin-from-gracilaria-lemaneiformis-by-centrifugal-precipitation-chromatography
#7
Dongyu Gu, Rodrigo Lazo-Portugal, Chen Fang, Zhantong Wang, Ying Ma, Martha Knight, Yoichiro Ito
Centrifugal precipitation chromatography (CpC) is a powerful chromatographic technique invented in the year 2000 but so far very little applied. The method combines dialysis, counter-current and salting out processes. The separation rotor consists of two identical spiral channels separated by a dialysis membrane (6-8 K MW cut-off) in which the upper channel is eluted with an ammonium sulfate gradient and the lower channel with water, and the mixtures are separated according to their solubility in ammonium sulfate as a chromatographic technique...
May 1, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29763746/ionic-liquid-type-surfactant-modified-attapulgite-as-a-novel-and-efficient-dispersive-solid-phase-material-for-fast-determination-of-pyrethroids-in-tea-drinks
#8
Xiaoling Yang, XiWen Lin, YiDuo Mi, Haixiang Gao, Jing Li, Sanbing Zhang, Wenfeng Zhou, Runhua Lu
In this study, a novel ionic liquid-type surfactant modified attapulgite named as 1-dodecyl-3-methylimidazolium bromide-attapulgite (C12 MIM-ATP) is successfully prepared and applied in dispersive solid phase extraction (dSPE) for the fast determination of pyrethroid residues in tea drinks. The primary factors that influenced the extraction efficiency, including sorbent type, amount of sorbent, extraction time, desorption conditions, pH and ionic strength, are investigated. The optimized results reveal that the extraction and desorption equilibria are rapidly obtained within 1 min...
April 30, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29734073/lateral-fluid-flow-fractionation-using-dielectrophoresis-lfff-dep-for-size-independent-label-free-isolation-of-circulating-tumor-cells
#9
Waqas Waheed, Anas Alazzam, Bobby Mathew, Nicolas Christoforou, Eiyad Abu-Nada
This short communication introduces a continuous-flow, dielectrophoresis-based lateral fluid flow fractionation microdevice for detection/isolation of circulating tumor cells in the presence of other haematological cells. The device utilizes two sets of planar interdigitated transducer electrodes micropatterned on top of a glass wafer using standard microfabrication techniques. A microchannel with a single inlet and two outlets, realized in polydimethylsiloxane, is bonded on the glass substrate. The two sets of electrodes slightly protrude into the microchannel...
April 30, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29730532/development-and-comparison-of-hplc-ms-ms-and-uplc-ms-ms-methods-for-determining-eight-coccidiostats-in-beef
#10
Xia Zhao, Bo Wang, Kaizhou Xie, Jianyu Liu, Yangyang Zhang, Yajuan Wang, Yawen Guo, Genxi Zhang, Guojun Dai, Jinyu Wang
A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method and an ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for determining eight coccidiostat (halofuginone, lasalocid, maduramicin, monensin, narasin, nigericin, robenidine and salinomycin) residues in beef were developed and compared. Samples were extracted with a mixture of acetic acid, acetonitrile and ethyl acetate and were then purified on a C18 solid-phase extraction (SPE) column. The purified samples were analyzed by HPLC-MS/MS and UPLC-MS/MS, using 0...
April 30, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29730530/fingerprint-profile-and-efficacy-associated-markers-of-nigella-sativa-oil-for-geographical-origin-determination-using-targeted-and-untargeted-hptlc-multivariate-analysis
#11
Eman Shawky, Nihal M El Newehy, Amira M Beltagy, Mohammad M Abd-Alhaseeb, Gamal A Omran, Fathallah M Harraz
Efficacy directed-fingerprint analysis of high-performance thin layer chromatography is proposed to set up fingerprint activity relationship modeling for precise discrimination of chemical and effective consistency of Nigella sativa oils from different geographical origins. A whole of 27 samples of N. sativa oils from three geographical area (Egypt, Ethiopia and Syria) were collected and their antimicrobial, cytotoxic, anti-inflammatory and analgesic activities were measured. The results revealed that there was significant difference in the biological activities of the oils collected...
April 30, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29747155/manual-punch-versus-automated-flow-through-sample-desorption-for-dried-blood-spot-lc-ms-ms-analysis-of-voriconazole
#12
Lisa C Martial, Erik van den Hombergh, Cornelis Tump, Otto Halmingh, David M Burger, Erik M van Maarseveen, Roger J Brüggemann, Rob E Aarnoutse
Dried blood spot (DBS) sampling is a patient-friendly alternative for plasma sampling for the purpose of therapeutic drug monitoring (TDM). To speed up the analysis time, an automated flow-through desorption method of DBS samples may be beneficial. This article describes the cross-validation of a manual punch DBS method with an automated desorption (DBS autosampler, DBSA) method for the DBS analysis of the antifungal drug voriconazole, followed by cross-validation of both DBS methods with a plasma-based method, and an assessment of agreement between DBS/DBSA and regular plasma concentration measurements (gold standard) in samples from patients on voriconazole treatment...
April 27, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29734072/a-dilute-and-shoot-multispectral-integration-approach-towards-nontargeted-component-profiling-of-traditional-herbal-yin-zhi-huang-using-liquid-chromatography-photodiode-array-ion-trap-time-of-flight-characterization
#13
Meiling Wang, Yu Liu, Bin Guo, Lijuan Wei, Hao Teng, Xiaoqing Chen, Ming Ma
Ying-zhi-huang Injection (YZH-I) is a classic intravenous formulation of polyherbal Chinese medicine which has been prescribed to treat severe jaundice and acute hepatitis for nearly 50 years. Despite some published data on its major components in the constituent herbs, the overall chemical profile of the potentially bioactive ingredients in YZH-I formula remains largely unknown. Here we developed a multispectral integration approach towards nontargeted phytochemical profiling of YZH-I by liquid chromatography-ultraviolet diode array detector coupled to ion trap/time-of-flight multistage mass spectrometry (LC-DAD-IT(MSn )/TOF) with fast polarity-switching mode...
April 27, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29738964/capillary-ultra-performance-liquid-chromatography-tandem-mass-spectrometry-analysis-of-tienilic-acid-metabolites-in-urine-following-intravenous-administration-to-the-rat
#14
Adam M King, Isobelle Grant, Paul D Rainville, Giorgis Isaac, Muireann Coen, Ian D Wilson, Robert S Plumb
Capillary scale (100 mm × 150 μm id) UPLC/MS/MS, performed using reversed-phase gradient chromatography on sub 2 μm particles, has been successfully employed for the characterization of the metabolites of the drug tienilic acid (TA) excreted via the urine following oral administration to the rat. The capillary LC system provided a significant increase (range ca. 11-33-fold) in sensitivity compared with a conventional 150 mm × 2.1 mm id UPLC system. An investigation of the effect of the injection volume and sample mass loading on the capillary column on the results obtained for both endogenous metabolites and TA was performed...
April 26, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29723699/measurement-of-pharmacokinetics-and-tissue-distribution-of-three-bioactive-constituents-from-zanthoxylum-armatum-dc-in-rat-plasma-and-tissues-through-uflc-ms-ms
#15
Yi-Ran Wang, Yong-Hui Li, Tao Guo, Hai-Long Li, Yin-Feng Tan, Zhong Zhang, Xu-Guang Zhang, Shi-Ying Mai, Jun-Qing Zhang
The compounds of N-Methylanhydrotetrahydroberberrubine A, dictamnine and eudesmin were the primary bioactive components in the roots of Zanthoxylum armatum DC (Z. armatum). To clarify the pharmacokinetics and distribution of these three compounds, an ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) was employed to determine the contents of these three compounds in rat plasma and seven tissues. The separation was achieved on a Kinetex XB-C18 100A column (2.1 × 50 mm, 2.6 μm, Phenomenex)...
April 26, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29738967/quantification-of-triacontanol-and-its-pegylated-prodrug-in-rat-plasma-by-gc-ms-ms-application-to-a-pre-clinical-pharmacokinetic-study
#16
Xiaoyu Lu, Min Fang, Yu Dai, Yue Yang, Ali Fan, Jiaqiu Xu, Zhiying Qin, Yang Lu, Di Zhao, Xijing Chen, Ning Li
PEGylation techniques have been increasingly employed in drug delivery system and chemical modification of compounds with low aqueous solubility. Triacontanol (TA) is a natural product with several pharmacological activities, but its low aqueous solubility significantly limited its application. PEGylated triacontanol (PEG-TA) was designed as the prodrug to improve the aqueous solubility and pharmacokinetic properties of TA. On the basis of salting-out assisted liquid-liquid extraction (SALLE) and saponification sample preparation procedure, a reliable gas chromatography tandem mass spectrometric (GC-MS/MS) method was developed and validated for the quantification of PEG-TA and its metabolite TA in rat plasma after separation and transformation...
April 24, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29709872/recent-technical-and-biological-development-in-the-analysis-of-biomarker-n-deoxyguanosine-c8-4-aminobiphenyl
#17
REVIEW
Zhidan Chen, Yuesheng Zhang, Paul Vouros
4-Aminobiphenyl (4-ABP) which is primarily formed during tobacco combustion and overheated meat is a major carcinogen responsible for various cancers. Its adducted form, N-deoxyguanosine-C8-4-aminobiphenyl (dG-C8-4-ABP), has long been employed as a biomarker for assessment of the risk for cancer. In this review, the metabolism and carcinogenisity of 4-ABP will be discussed, followed by a discussion of the current common approaches of analyzing dG-C8-4-ABP. The major part of this review will be on the history and recent development of key methods for detection and quantitation of dG-C8-4-ABP in complex biological samples and their biological applications, from the traditional 2 P-postlabelling and immunoassay methods to modern liquid chromatography-mass spectrometry (LC-MS) with the latter as the focus...
April 24, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29704798/validation-of-an-lc-ms-ms-method-for-simultaneous-quantification-of-venlafaxine-and-its-five-metabolites-in-rat-plasma-and-its-application-in-a-pharmacokinetic-study
#18
Guodong Gu, Michelle Black, Colt Cookson, Anna Fiorella, Yinghe Li, Steven H Gorman, Ray Bakhtiar
A sensitive, selective, and reliable LC-MS/MS method was developed and validated for simultaneous quantification of venlafaxine (VEN) and its 5 metabolites (ODV, NDV, NNDDV, OHV and NODDV) in rat plasma. The calibration ranges are 15.0 to 6000 ng/mL for VEN, 1.00 to 400 ng/mL for ODV, 5.00 to 2000 ng/mL for NDV, 1.00 to 400 ng/mL for NNDDV, 10.0 to 4000 ng/mL for OHV, and 0.200 to 20.0 ng/mL for NODDV. Briefly, 50 μL of rat plasma was extracted using liquid-liquid extraction (LLE) with methyl tert-butyl ether (MTBE)...
April 23, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29738965/arginine-homopeptides-for-plasmid-dna-purification-using-monolithic-supports
#19
Sara Cardoso, Ângela Sousa, João A Queiroz, Adriano R Azzoni, Fani Sousa
Purification of plasmid DNA targeting therapeutic applications still presents many challenges, namely on supports and specific ligand development. Monolithic supports have emerged as interesting approaches for purifying pDNA due to its excellent mass transfer properties and higher binding capacity values. Moreover, arginine ligands were already described to establish specific and preferential interactions with pDNA. Additionally, some studies revealed the ability of arginine based cationic peptides to condense plasmid DNA, which increased lengthening can result in strongest interactions with higher binding capacities for chromatographic purposes of large molecules such as pDNA...
April 22, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29702354/the-enrichment-of-chlorogenic-acid-from-eucommia-ulmoides-leaves-extract-by-mesoporous-carbons
#20
Guotong Qin, Jing Ma, Wei Wei, Jaja Li, Fangqing Yue
Herein, we report an efficient separation and enrichment method for chlorogenic acid from crude extracts of Eucommia ulmoides leaves using carbon adsorbents. The effects of the pore structure of the carbon adsorbents on the adsorption capacity were studied. Of the four adsorbents investigated, mesoporous carbon (MC3) showed the highest adsorption capacity (294 mg/g of carbon) for chlorogenic acid due to its high mesopore volume. The static adsorption of CGA on carbon can be accurately described using the Freundlich equation...
April 22, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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