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Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

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https://www.readbyqxmd.com/read/30415146/studying-drug-drug-interaction-through-chromatographic-analysis-of-two-mixtures-offering-antimicrobial-synergism
#1
Dina El Sherbiny, Mary E K Wahba
Micellar mobile phase was utilized for the separation and quantification of two antimicrobial mixtures with some analgesics that were found to augment their antimicrobial activity, namely; cefotaxime sodium (CFX) with ibuprofen (IBU) and naproxen (NPX) (mixture Ι), and azithromycin dihydrate (AZT) with diclofenac sodium (DIC) (mixture ΙΙ). Both mixtures were analyzed using a C18 column operated at 50 °C using a mobile phase composed of sodium dodecyl sulphate (SDS), an organic modifier; (1-propanol or acetonitrile), and 0...
November 3, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30396050/quantitative-bioanalytical-assay-for-the-tropomyosin-receptor-kinase-inhibitor-larotrectinib-in-mouse-plasma-and-tissue-homogenates-using-liquid-chromatography-tandem-mass-spectrometry
#2
Rolf W Sparidans, Yaogeng Wang, Alfred H Schinkel, Jan H M Schellens, Jos H Beijnen
Larotrectinib is a promising tyrosine kinase inhibitor for solid tumors harboring tropomyosin receptor kinase gene fusions. A bioanalytical assay was developed for this drug in small volume samples using a 96-well format to efficiently support multiple mouse studies. The assay was completely validated for mouse plasma and partially for homogenates of eight different tissues: brain, heart, kidneys, liver, lungs, small intestine, spleen, and testes. Proteins in 10-μl samples were precipitated using acetonitrile containing momelotinib as internal standard...
October 30, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30391728/cell-metabolomics-identify-regulatory-pathways-and-targets-of-magnoline-against-prostate-cancer
#3
Hui Sun, Ai-Hua Zhang, Shao-Bo Liu, Shi Qiu, Xian-Na Li, Tian-Lei Zhang, Liang Liu, Xi-Jun Wang
Prostate cancer is known as a common malignant tumor in clinics and moreover, traditional chemotherapeutic drugs have great toxic side effects and drug resistance. Therefore, the searching the highly efficient and low toxicity antitumor drugs from natural drugs has become an important approach for the treatment of prostate cancer. Many studies showed that Cortex Phellodendri has important therapeutic significance for prostate cancer. Magnoline is the main component of Cortex Phellodendri Amurensis, and it is of great significance to evaluate the effect of magnoline on prostate cancer...
October 27, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30384187/purification-of-recombinant-human-butyrylcholinesterase-on-hupresin%C3%A2
#4
Oksana Lockridge, Emilie David, Lawrence M Schopfer, Patrick Masson, Xavier Brazzolotto, Florian Nachon
Affinity chromatography on procainamide-Sepharose has been an important step in the purification of butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) since its introduction in 1978. The procainamide affinity gel has limitations. In the present report a new affinity gel called Hupresin® was evaluated for its ability to purify truncated, recombinant human butyrylcholinesterase (rHuBChE) expressed in a stably transfected Chinese Hamster Ovary cell line. We present a detailed example of the purification of rHuBChE secreted into 3940 mL of serum-free culture medium...
October 25, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30380468/chromatographic-separation-strategies-for-precision-mass-spectrometry-to-study-protein-protein-interactions-and-protein-phosphorylation
#5
REVIEW
Zoran Minic, Tanya E S Dahms, Mohan Babu
Investigating protein-protein interactions and protein phosphorylation can be of great significance when studying biological processes and human diseases at the molecular level. However, sample complexity, presence of low abundance proteins, and dynamic nature of the proteins often impede in achieving sufficient analytical depth in proteomics research. In this regard, chromatographic separation methodologies have played a vital role in the identification and quantification of proteins in complex sample mixtures...
October 24, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30391730/expanding-the-applicability-of-cork-as-extraction-phase-for-disposable-pipette-extraction-in-multiresidue-analysis-of-pharmaceuticals-in-urine-samples
#6
Gabriela Mafra, Daniel Spudeit, Rafael Brognoli, Josias Merib, Eduardo Carasek
In this paper, cork is proposed as a natural and renewable material for the extraction phase in disposable pipette extraction to be applied in the multiresidue determination of pharmaceuticals in human urine by HPLC with diode array detection. The compounds carbamazepine, losartan, ketoprofen, 17‑β‑estradiol, naproxen, diazepam, 17‑α‑ethinylestradiol, estrone, diclofenac and ibuprofen were studied. A known amount (5 mg) of cork, with a size of 200 mesh, was inserted into a pipette tip. The method optimization was carried out with univariate and multivariate approaches...
October 23, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30388703/optimized-bio-analytical-methods-development-and-comparative-pharmacokinetic-studies-of-four-antidepressants-in-egyptian-population-based-on-gender-difference
#7
Ehab F ElKady, Ahmed A Abo Elwafa, Faten Farouk
The pharmacokinetics (PK) and pharmacodynamics of many oral antidepressants (OADs) vary substantially among different genders and ethnicities. Likewise is their therapeutic effectiveness, time to response and the incidence of adverse drug events. The aim of this study is to compare the PK of four OADs (desvenlafaxine; DSV, venlafaxine; VLX, escitalopram; ESP, and agomelatine; AGT) among Egyptian males and females. In this study, LC-MS/MS methods were developed and validated for determining the four OADs in human plasma...
October 22, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30384188/rapid-bioanalytical-lc-ms-ms-method-for-the-simultaneous-determination-of-sofosbuvir-and-velpatasvir-in-human-plasma-application-to-a-pharmacokinetic-study-in-egyptian-volunteers
#8
Ehab F Elkady, Ahmed A Aboelwafa
A novel, rapid and validated LC-MS/MS bioanalytical method has been developed for the extraction and determination of sofosbuvir and velpatasvir simultaneously in human plasma using ledipasvir as an internal standard (IS). The simple and reproducible protein precipitation technique with acetonitrile was successfully used for the deproteinization and extraction of the analytes from human plasma matrix. The developed method achieved consistent recoveries over different concentrations with average extraction recoveries of 81...
October 22, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30380467/identification-and-quantification-of-product-related-quality-attributes-in-bio-therapeutic-monoclonal-antibody-via-a-simple-and-robust-cation-exchange-hplc-method-compatible-with-direct-online-detection-of-uv-and-native-esi-qtof-ms-analysis
#9
Praveen Kallamvalliillam Sankaran, Pradeep G Kabadi, Chethan Gejjalagere Honnappa, Malini Subbarao, Harish V Pai, Laxmi Adhikary, Dinesh V Palanivelu
Modern analytical ion-exchange chromatography is one of the conventional tools used for assessment of product-related quality attributes in bio-therapeutic monoclonal antibodies (mAbs). Here, we present an approach to resolve, identify, and quantify product-related substances of therapeutic mAb at its intact molecular level by cation exchange (CIEX) HPLC coupled directly to electrospray ionization - quadrupole time of flight mass spectrometry (ESI-QTOF-MS). This method utilizes pH gradient elution mode comprised of ammonium formate buffer components, and a weak cation exchange column as stationary phase...
October 21, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30366208/a-direct-sensitive-and-efficient-method-for-determination-of-alpha-fluoro-beta-alanine-in-urine-evaluating-the-influence-of-magnesium-isoglycyrrhizinate-on-excretion-in-rat-model
#10
Zhipeng Wang, Yang Yang, Feng Zhang, Mingming Li, Jing Chen, Huan Man, Wei Jiang, Rui Zhang, Shouhong Gao, Wansheng Chen
Alpha-fluoro-beta-alanine (FBAL), the final metabolite of capecitabine, is a toxic compound excreting with urine. Magnesium isoglycyrrhizinate injection is a traditional Chinese medicine prescribed with capecitabine as a hepatoprotective agent. The purposes of this study are to develop an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for direct, efficient and sensitive determination of FBAL in urine and explore the influence of magnesium isoglycyrrhizinate on the excretion of FBAL in rat model...
October 18, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30391729/pharmacokinetic-study-comparing-pure-desoxo-narchinol-a-and-nardosinonediol-with-extracts-from-nardostachys-jatamansi
#11
Vu Ngoc Han Le, Yan Zhao, Chong Woon Cho, MinKyun Na, Khong Trong Quan, Jang Hoon Kim, Sung Yeoun Hwang, Sang Wook Kim, Kyung Tae Kim, Jong Seong Kang
Nardostachyos Radix et Rhizoma (NR) is a valuable medicinal herb widely used in Korea, India, and China for the treatment of many diseases. Desoxo-narchinol A (DA) and nardosinonediol (ND) are the two main bioactive compounds belonging to the sesquiterpene group. Desoxo-narchinol A possesses anti-inflammatory activity while ND exhibits anti-depressant and cardioprotective activities. A pharmacokinetic study is important to decide whether the isolated compounds or the NR extract have better pharmacological activity...
October 17, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30380466/design-of-experiments-based-rp-hplc-bioanalytical-method-development-for-estimation-of-rufinamide-in-rat-plasma-and-brain-and-its-application-in-pharmacokinetic-study
#12
Avantika V Dalvi, Chandra Teja Uppuluri, Ekta Prasanthi Bommireddy, Punna Rao Ravi
This study reports a fully validated HPLC-UV (High Performance Liquid Chromatography- Ultra Violet) method for quantitative estimation of Rufinamide (RUFI), an antiepileptic drug, in rat plasma and brain matrices. A response surface methodology based Box Behnken experimental design, using the principles of Design of Experiments (DoE), was employed to optimize critical chromatographic conditions viz. pH and proportion of the buffer and wavelength of detection, for achieving good sensitivity (peak area) and specificity (number of theoretical plates)...
October 17, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30366211/binding-studies-based-on-ultrafast-affinity-extraction-and-single-or-two-column-systems-interactions-of-second-and-third-generation-sulfonylurea-drugs-with-normal-or-glycated-human-serum-albumin
#13
Bao Yang, Xiwei Zheng, David S Hage
Ultrafast affinity extraction was evaluated and used with microcolumns containing human serum albumin (HSA) to measure the global affinity constants and dissociation rate constants for several second- and third-generation sulfonylurea drugs with solution-phase normal HSA or glycated HSA. Glibenclamide, glimepiride and glipizide were used as model drugs for this work. Both single- and two-column systems were considered for the analysis of global affinities for the model drugs. These methods were optimized with respect to the flow rates, column sizes and sample residence times that were employed with each drug for ultrafast affinity extraction...
October 17, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30366209/intervention-effect-of-qi-yu-san-long-decoction-on-lewis-lung-carcinoma-in-c57bl-6-mice-insights-from-uplc-qtof-ms-based-metabolic-profiling
#14
Huan Wu, Yang Chen, Qinglin Li, Yating Gao, Xingxing Zhang, Jiabing Tong, Zhiqiang Zhang, Jian Hu, Danyang Wang, Shijie Zeng, Zegeng Li
Qi-Yu-San-Long Decoction (QYSLD) has been used to treat lung carcinoma for over twenty years in clinical practices, and its curative effect is considered credible. However, the therapeutic mechanism of this effect has not been thoroughly elucidated to date. In this study, a MTT dye reduction assay and DAPI staining were first used to evaluate the cell viability and apoptosis of A549 cells with and without QYSLD-treatment, respectively. The weight/volume of Lewis lung carcinoma (LLC) sarcoma was used to assess the therapeutic effect of QYSLD on LLC mice...
October 17, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30368043/a-normal-phase-high-performance-liquid-chromatography-method-for-the-separation-of-hydroxy-and-non-hydroxy-neutral-lipid-classes-compatible-with-ultraviolet-and-in-line-liquid-scintillation-detection-of-radioisotopes
#15
Hari Kiran Kotapati, Philip D Bates
In this paper, we report a method for the separation of hydroxy fatty acid and non-hydroxy fatty acid containing neutral lipid classes via normal phase HPLC with UV detection on a PVA-Sil column. The hexane/isopropanol/methanol/water based method separates all the neutral lipids in 21 min, and subsequently flushes through the polar lipids by 27 min such that prefractionation of neutral and polar lipids are not required, and the column is re-equilibrated for the next run in 15 min, for a total run time of 45 min per sample...
October 15, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30336346/multi-milligram-resolution-and-determination-of-absolute-configuration-of-pentedrone-and-methylone-enantiomers
#16
Bárbara Silva, José A Pereira, Sara Cravo, Ana Margarida Araújo, Carla Fernandes, Madalena M M Pinto, Paula Guedes de Pinho, Fernando Remião
The enantioresolution of pentedrone and methylone was carried out at a multi-milligram scale by liquid chromatography on a Chiralpak AS® stationary phase. The excellent enantioresolution using this column allowed to collect highly pure enantiomeric fractions, achieving enantiomeric ratios higher than 98%. An overall recovery of 72% was achieved for pentedrone enantiomers and 80% for methylone. Furthermore, the absolute configuration of the enantiomers of both cathinones was determined for the first time by electronic circular dichroism (ECD) spectroscopy, with the aid of theoretical calculations, as (+)‑(S) and (-)‑(R)-pentedrone, and (-)‑(S) and (+)‑(R)‑methylone...
October 15, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30366210/a-uhplc-qtof-ms-ms-method-for-the-simultaneous-determination-of-eight-triterpene-compounds-from-poria-cocos-schw-wolf-extract-in-rat-plasma-application-to-a-comparative-pharmacokinetic-study
#17
Qi Qian, Na Zhou, Pengcheng Qi, Yuqian Zhang, Xiyan Mu, Xiaowei Shi, Qiao Wang
Poria cum Radix Pini (PRP), White Poria (WP), Rubra Poria (RP), and Poriae Cutis (PC), different parts of the dried sclerotium of Poria cocos (Schw.) Wolf (PCW), have possessed various pharmacological effects and clinical application. In the present study, a novel ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS) method was developed and validated for the simultaneous determination of eight triterpene compounds in rat plasma and then was applied in the comparison of pharmacokinetic characteristics of PRP, WP, RP, and PC extracts...
October 13, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30380465/screening-of-fatty-acids-saccharides-and-phytochemicals-in-jatropha-curcas-seed-kernel-as-their-trimethylsilyl-derivatives-using-gas-chromatography-mass-spectrometry
#18
Hoa Truong Thi, Bang Anh Le, Hanh Ngoc Thi Le, Kenji Okitsu, Kiyoshi Imamura, Norimichi Takenaka, Boi Van Luu, Yasuaki Maeda
Jatropha curcas is a multipurpose plant, of which the seed kernel oil (up to 60% content) has been exploited for BDF production. In this report, we explored the various kinds of minor compounds of saccharides, phytochemicals, fatty acids (FAs), and amino acids in the seed kernel using gas chromatography/mass spectrometry (GC/MS) as their trimethylsilyl (TMS) derivatives. The homogenized seed kernels were extracted with methanol, and the extract was distributed into ethyl acetate/water phase. The components of each layer were derivatized with N, O-bis (trimethylsilyl) trifluoroacetamide (BSTFA) and their TMS derivatives were screened by GC/MS analysis...
October 12, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30368044/a-validated-hplc-ms-ms-method-for-estimating-the-concentration-of-the-ganglioside-g-d2-in-human-plasma-or-serum
#19
C M Busch, A V Desai, G S Moorthy, E Fox, F M Balis
GD2 is a ganglioside found in the plasma membrane of the neural crest-derived cancer, neuroblastoma. GD2 is shed into the circulation of patients with neuroblastoma and could serve as a tumor biomarker to monitor tumor burden or response to treatment. We developed and validated a high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method to quantify the D18:1-18:0 (C18) and the D18:1-20:0 (C20) lipoforms of GD2 in human plasma and serum. Human brain derived GD2 containing a mixture of C18 and C20 was used as the analytical standard...
October 12, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30340066/investigation-of-n-g-hydroxy-l-arginine-interference-in-the-quantitative-determination-of-nitrite-and-nitrate-in-human-plasma-and-urine-by-gc-nici-ms
#20
Alexander Bollenbach, Erik Hanff, Dimitrios Tsikas
NG -Hydroxy-l-arginine (NOHA) is the intermediate product of the conversion of l-arginine to l-citrulline and nitric oxide (NO) by nitric oxide synthase (NOS). NO is further oxidized to nitrite and nitrate which circulate in the blood and are excreted in the urine. Nitrite and nitrate may therefore serve as surrogates of NO synthesis. NOHA has been reported to occur in various cells and in blood of animals and humans. The concentration of nitrite in the circulation is comparable to the concentration of NOHA in plasma and serum of humans and laboratory animals...
October 9, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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