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Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

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https://www.readbyqxmd.com/read/29153998/determination-of-isoquercitrin-in-rat-plasma-by-high-performance-liquid-chromatography-coupled-with-a-novel-synergistic-cloud-point-extraction
#1
Jun Zhou, Jiang Bing Sun, Qiao Feng Wang
A novel improved preconcentration method known as synergistic cloud point extraction was established for isoquercitrin preconcentration and determination in rat plasma prior to its determination by high performance liquid chromatography. Synergistic cloud point extraction greatly simplified isoquercitrin extraction and detection. This method was accomplished at room temperature (about 22°C) in 1min with the nonionic surfactant Tergitol TMN-6 as the extractant, n-octanol as cloud point revulsant and synergic reagent...
November 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29153997/first-investigations-for-the-characterization-of-glucosamine-6-phosphate-synthase-by-capillary-electrophoresis
#2
Miriam Beneito-Cambra, Pierre Gareil, Bernard Badet, Marie-Ange Badet-Denisot, Nathalie Delaunay
The enzyme glucosamine-6-phosphate synthase (GlmS) is an important point of metabolic control in biosynthesis of amino sugar-containing macromolecules and is therefore a potential target in order to design antibacterial and antifungal drugs. It has two oligomerization states, which are the active dimer and the inactive hexamer. For the first time, the potential of CE to separate and quantify the two forms was studied. After incubating GlmS with the d-glucosamine 6-phosphate (GlcN6P) inhibitor, an electrolyte based on sodium phosphate at pH 7...
November 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29149736/graphene-oxide-reinforced-ionic-liquid-functionalized-adsorbent-for-solid-phase-extraction-of-phenolic-acids
#3
Xiudan Hou, Xiaofeng Lu, Sheng Tang, Licheng Wang, Yong Guo
An environmental friendly sorbent of polymeric ionic liquids modified graphene oxide-grafted silica (PILs@GO@Sil) was synthesized for solid-phase extraction (SPE) of phenolic acids. The sorbent was prepared via a chemical layer-to-layer fabrication including amidation reaction, surface radical chain-transfer polymerization and in situ anion exchange. After modification with PILs, the silica surface had higher positive potential so that it would exhibit stronger electrostatic interaction for acidic compounds compared with GO@Sil...
November 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29145024/direct-analysis-of-ethylene-glycol-in-human-serum-on-the-basis-of-analyte-adduct-formation-and-liquid-chromatography-tandem-mass-spectrometry
#4
Marek Dziadosz
The aim of this work was to develop a fast, cost-effective and time-saving liquid chromatography-tandem mass spectrometry (LC-MS/MS) analytical method for the analysis of ethylene glycol (EG) in human serum. For these purposes, the formation/fragmentation of an EG adduct ion with sodium and sodium acetate was applied in the positive electrospray mode for signal detection. Adduct identification was performed with appropriate infusion experiments based on analyte solutions prepared in different concentrations...
November 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29153999/improving-the-selectivity-and-sensitivity-for-quantifying-8-%C3%AE-hydroxy-mutilin-in-rabbit-tissues-by-using-basic-mobile-phases-and-negative-ionization
#5
Aimin Tan, Guifen Gu, Xuan Gui, Yanxin Wu, Gordon Bolger, Albert Licollari, John C Fanaras
Previously reported LC-MS methods for quantifying 8-α-hydroxy-mutilin (a marker residue of tiamulin) in tissues all used a pseudo MRM transition (from protonated molecular ion to protonated molecular ion, m/z 337→337) due to difficulties in finding a product ion, leading to suboptimal selectivity and sensitivity for detection. By using electrospray negative ionization in a basic medium, we, for the first time, found a highly selective and sensitive true MRM transition for 8-α-hydroxy-mutilin, m/z 335→179...
November 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29145025/coupling-of-on-column-trypsin-digestion-peptide-mapping-and-principal-component-analysis-for-stability-and-biosimilarity-assessment-of-recombinant-human-growth-hormone
#6
Sara M Shatat, Basma M Eltanany, Abeer A Mohamed, Medhat A Al-Ghobashy, Faten A Fathalla, Samah S Abbas
Peptide mapping (PM) is a vital technique in biopharmaceutical industry. The fingerprint obtained helps to qualitatively confirm host stability as well as verify primary structure, purity and integrity of the target protein. Yet, in-solution digestion followed by tandem mass spectrometry is not suitable as a routine quality control test. It is time consuming and requires sophisticated, expensive instruments and highly skilled operators. In an attempt to enhance the fuctionality of PM and extract multi-dimentional data about various critical quality attributes and comparability of biosimilars, coupling of PM generated using immobilized trypsin followed by HPLC-UV to principal component analysis (PCA) is proposed...
November 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29132023/quantification-of-fluorescent-dyes-in-organ-tissue-samples-via-hplc-analysis
#7
S Morsbach, A García-Bardon, J Kamuf, B Müller, N Beghersa, K Mohr, K Landfester
The determination of regional blood flow via the accumulation of fluorescent microspheres is a concept regularly used in medical research. Typically, the microbeads get extracted from the tissue of interest and are then quantified by measuring the absorption or fluorescence of the incorporated dyes without further separation from the medium. However, in that case the absorption spectra of different dyes can overlap when used simultaneously, leading to an overestimation of the concentration. Additionally, background absorption from the medium can be problematic...
November 10, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29129604/simultaneous-determination-of-nine-kinds-of-dominating-bile-acids-in-various-snake-bile-by-ultrahigh-performance-liquid-chromatography-with-triple-quadrupole-linear-iontrap-mass-spectrometry
#8
Jie Zhang, Yeqin Fan, Yajun Gong, Xiaoyong Chen, Luosheng Wan, Chenggao Zhou, Jiewen Zhou, Shuangcheng Ma, Feng Wei, Jiachun Chen, Jing Nie
Snake bile is one of the most expensive traditional Chinese medicines (TCMs). However, due to the complicated constitutes of snake bile and the poor ultraviolet absorbance of some trace bile acids (BAs), effective analysis methods for snake bile acids were still unavailable, making it difficult to solve adulteration problems. In present study, ultrahigh-performance liquid chromatography with triple quadrupole linear ion trap mass spectrometry (UHPLC-QqQ-MS/MS) was applied to conduct a quantitative analysis on snake BAs...
November 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29136552/development-and-validation-of-modified-quechers-method-coupled-with-lc-ms-ms-for-simultaneous-determination-of-cymiazole-fipronil-coumaphos-fluvalinate-amitraz-and-its-metabolite-in-various-types-of-honey-and-royal-jelly
#9
Weijia Zheng, Jin-A Park, A M Abd El-Aty, Seong-Kwan Kim, Sang-Hyun Cho, Jeong-Min Choi, Hee Yi, Soo-Min Cho, Amer Ramadan, Ji Hoon Jeong, Jae-Han Shim, Ho-Chul Shin
Over the past few decades, honey products have been polluted by different contaminants, such as pesticides, which are widely applied in agriculture. In this work, a modified EN - quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method was developed for the simultaneous quantification of pesticide residues, including cymiazole, fipronil, coumaphos, fluvalinate, amitraz, and its metabolite 2,4-dimethylaniline (2,4-DMA), in four types of honey (acacia, wild, chestnut, and manuka) and royal jelly...
November 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29132025/micro-quechers-extraction-coupled-to-gc-ms-for-a-fast-determination-of-bisphenol-a-in-human-urine
#10
Luísa Correia-Sá, Sónia Norberto, Cristina Delerue-Matos, Conceição Calhau, Valentina F Domingues
Bisphenol A (BPA) is considered an endocrine disruptor and public concern over BPA exposure has been raised. Several studies have assessed human exposure to this plasticizer, confirming its ubiquitous presence and highlighting children as a public of special concern. A simple, efficient, cheap and green analytical procedure is reported within this paper. This paper reports, for the first time, the development of a modified Micro-QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method coupled to gas chromatography-mass spectrometry (GC-MS) as a new strategy for the efficient extraction and determination of Bisphenol A in human urine samples...
November 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29136555/non-targeted-metabolomics-guided-sildenafil-metabolism-study-in-human-liver-microsomes
#11
Ju-Hyun Kim, Jun Hyun Jo, Kyung-Ah Seo, Hayoung Hwang, Hye Suk Lee, Sangkyu Lee
Metabolomics combined with high-resolution mass spectrometry (HR-MS) and multivariate data analysis has broad applications in the study of xenobiotic metabolism. Although information about xenobiotic metabolism is essential to understand toxic mechanisms, pharmacokinetic parameters and excretion pathways, it is limited to predict all generated metabolites in biological fluids. Here, we revisited sildenafil metabolism in human liver microsomes using a metabolomics approach to achieve a global picture of sildenafil phase 1 metabolism...
November 7, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29136554/separation-and-purification-of-four-phenolic-compounds-from-persimmon-by-high-speed-counter-current-chromatography
#12
Jinming Peng, Kaikai Li, Wei Zhu, Xiangyi Deng, Chunmei Li
An efficient method was established by high-speed counter-current chromatography (HSCCC) for preparation of four phenolic compounds from the depolymerization products of persimmon tannin. Using the two solvent systems of n-hexane/ethyl acetate/water (3:17:20, v/v/v) and ethyl acetate/methanol/water (50:1:50, v/v/v), the preparative isolation was successfully performed by a two-step separation. The yields of one run (150mg crude sample) for gallic acid, methyl gallate, and epigallocatechin-3-gallate-(4β→8, 2β→O→7)-epigallocatechin-3-gallate dimer (A-type EGCG dimer) were 4...
November 7, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29145026/lc-ms-ms-quantification-of-felinine-metabolites-in-tissues-fluids-and-excretions-from-the-domestic-cat-felis-catus
#13
Ayami Futsuta, Wataru Hojo, Tamako Miyazaki, Tetsuro Yamashita, Masao Miyazaki
Domestic cat urine contains large concentrations of the unusual amino acid, felinine (2-amino-7-hydroxy-5,5-dimethyl-4-thiaheptanoic acid). A felinine derivative, 3-mercapt-3-methylbutanol is a potential scent signal for letting other animals know that the scent owners are cats. 3-Methylbutanol-glutathione (MBG) is an upstream precursor of the biosynthetic pathway of felinine that may be produced in hepatocytes by conjugation of glutathione with isopentenyl pyrophosphate, an intermediate for cholesterol biosynthesis...
November 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29132022/determination-of-perfluoroalkyl-acid-isomers-in-biosolids-biosolids-amended-soils-and-plants-using-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#14
Hongna Zhang, Bei Wen, Wen Wen, Yibing Ma, Xiaoyu Hu, Yali Wu, Lei Luo, Shuzhen Zhang
Isomer-specific analysis of perfluoroalkyl acids (PFAAs) is important to accurately assess their environmental source, fate, and human risks. In this study, a method was developed for the determination of perfluorooctanoic acid (PFOA), perfluorooctane sulfonate (PFOS), and perfluorohexane sulfonate (PFHxS) isomers in biosolids, biosolids-amended soils and plants using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The separation efficiencies of two chromatographic columns and extraction capacities of different methods were tested...
November 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29129603/a-rapid-quantitative-analysis-of-bile-acids-lysophosphatidylcholines-and-polyunsaturated-fatty-acids-in-biofluids-based-on-ultraperformance-liquid-chromatography-coupled-with-triple-quadrupole-tandem-massspectrometry
#15
Zhangxiao Peng, Qian Zhang, Ziming Mao, Jie Wang, Chunying Liu, Xuejing Lin, Xin Li, Weidan Ji, Jianhui Fan, Maorong Wang, Changqing Su
Much evidence suggested that quantitative analysis of bile acids (BAs), lysophosphatidylcholines (LPCs), and polyunsaturated fatty acids (PUFAs) in biofluids may be very useful for diagnosis and prevention of hepatobiliary disease with a non-invasive manner. However, simultaneously fast analysis of these metabolites has been challenging for their huge differences of physicochemical properties and concentration levels in biofluids. In this study, we present a liquid chromatography-mass spectrometry method with a high throughput analytical cycle (10min) to fast and accurately quantify fifteen potential biomarkers (eight BAs, four LPCs and three PUFAs) of hepatobiliary disease...
November 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29157782/comparative-assessment-of-the-effect-of-hyper-glycosylation-on-the-pattern-and-kinetics-of-degradation-of-darbepoetin-alfa-using-a-stability-indicating-orthogonal-testing-protocol
#16
Eman M Moenes, Medhat A Al-Ghobashy, Abeer A Mohamed, Maissa Y Salem
Darbepoetin alfa (DA); hyper-glycosylated Erythropoietin alfa (EPO) is an essential treatment of anemia in patients with chronic kidney failure and cancer. In this study, DA and EPO were subjected to physicochemical stress factors that might be encountered during production, transport and storage (pH, temperature, agitation, repeated freeze-thaw and oxidation). An orthogonal stability-indicating assay protocol comprised of SE-HPLC, RP-HPLC, ELISA and SDS-PAGE was developed and validated to investigate the effect of further glycosylation of DA on the pattern and kinetics of degradation...
November 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29149735/carbamazepine-lamotrigine-levetiracetam-and-valproic-acid-in-dried-blood-spots-with-liquid-chromatography-tandem-mass-spectrometry-method-development-and-validation
#17
Camilla Linder, Anna Hansson, Sara Sadek, Lars L Gustafsson, Anton Pohanka
Monitoring of antiepileptic drugs in children with epilepsy require multiple visits at a clinic for blood collection. Dried blood spot sampling is an alternative way of collection, performed at home by self-collection and can save time and costs for patients and family members. The aim was to develop and validate an LC-MS/MS dried blood spot method for carbamazepine, lamotrigine, levetiracetam and valproic acid with the requirements of using standard equipment and material in a routine laboratory setting. Whatman-903 filter paper was utilized, and discs were punched into a 96 well plate with an automated puncher and barcode reading...
November 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29132020/separation-of-aflatoxin-b1-from-synthetic-physiological-fluids-using-talc-and-diatomite-kinetic-and-isotherm-aspects
#18
Myroslav Sprynskyy, Iwona Krzemień-Konieczka, Renata Gadzała-Kopciuch, Bogusław Buszewski
The objective of the study was to examine adsorption of the aflatoxin B1 from synthetic gastric fluid and synthetic intestinal fluid by talc, raw and calcined diatomite. The kinetic and equilibrium adsorption processes were studied in the batch adsorption experiments applying high performance liquid chromatography for the aflatoxin B1 determination. The kinetic study showed a very fast adsorption of the aflatoxin B1 onto the selected adsorbents from the both physiological fluids with reaching equilibrium within 1-15min...
November 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29128277/a-fast-sensitive-and-high-throughput-method-for-the-determination-of-esomeprazole-in-dog-plasma-by-uhplc-ms-ms-application-to-formulation-development-of-the-compound-preparation-of-esomeprazole
#19
Ran Liu, Pei Li, Jie Xiao, Yidi Yin, Zheng Sun, Kaishun Bi, Qing Li
To investigate deeply into the preclinical pharmacokinetics and prescription design of esomeprazole, a sensitive, high throughput and robust UHPLC-MS/MS method had been developed and fully validated for the analysis of esomeprazole in dog plasma. Esomeprazole and diazepam (IS) were fast extracted from plasma by alkalified organic solvent, and separated on MP-C18 column with methanol and 0.1% formic acid. The quantification of esomeprazole and IS had been achieved using fragmentation transitions of m/z 346.1→198...
November 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29128276/simultaneous-determination-of-saikosaponin-a-paeonol-and-imperatorin-components-of-da-9805-in-rat-plasma-by-lc-ms-ms-and-application-to-a-pharmacokinetic-study
#20
Mi Hye Kwon, Jin Seok Jeong, Jayoung Ryu, Young Woong Cho, Hee Eun Kang
DA-9805 is a new botanical antiparkinson drug candidate formulated using an ethanolic extract of the root of Bupleurum falcatum, the root cortex of Paeonia suffruticosa, and the root of Angelica dahurica. In this study, a sensitive and rapid LC-MS/MS method was developed to simultaneously determine, saikosaponin a, paeonol, and imperatorin, three active/representative ingredients of DA-9805, in rat plasma. Plasma was extracted by mixture of ethyl acetate and methyl tertiary butyl ether. Chromatographic separation was carried out using a C18 column and a gradient elution of mobile phases consisting of 5mM formic acid in water and acetonitrile...
November 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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