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Journals International Journal of Mass ...

International Journal of Mass Spectrometry

https://read.qxmd.com/read/38352886/nonlinear-frequency-modulation-for-fourier-transform-ion-mobility-mass-spectrometry-improves-experimental-efficiency
#1
JOURNAL ARTICLE
Elvin R Cabrera, Robert L Schrader, Thomas E Walker, Arthur Laganowsky, David H Russell, Brian H Clowers
Through optimization of terminal frequencies and effective sampling rates, we have developed nonlinear sawtooth-shaped frequency sweeps for efficient Fourier transform ion mobility mass spectrometry (FT-IM-MS) experiments. This is in contrast to conventional FT-IM-MS experiments where ion gates are modulated according to a linear frequency sweep. Linear frequency sweeps are effective but can be hindered by the amount of useful signal obtained using a single sweep over a large frequency range imposed by ion gating inefficiencies, particularly small ion packets, and gate depletion...
March 2024: International Journal of Mass Spectrometry
https://read.qxmd.com/read/38053979/triboelectric-nanogenerators-low-cost-power-supplies-for-improved-electrospray-ionization
#2
JOURNAL ARTICLE
Daniel D Vallejo, Joseph L Corstvet, Facundo M Fernández
Electrospray ionization (ESI) is one of the most popular methods to generate ions for mass spectrometry (MS). When compared with other ionization techniques, it can generate ions from liquid-phase samples without additives, retaining covalent and non-covalent interactions of the molecules of interest. When hyphenated to liquid chromatography, it greatly expands the versatility of MS analysis of complex mixtures. However, despite the extensive growth in the application of ESI, the technique still suffers from some drawbacks when powered by direct current (DC) power supplies...
January 2024: International Journal of Mass Spectrometry
https://read.qxmd.com/read/38009161/absolute-quantitation-of-peptides-and-proteins-by-coulometric-mass-spectrometry-after-derivatization
#3
JOURNAL ARTICLE
Praneeth Ivan Joel Fnu, Md Tanim-Al-Hassan, Timothy Yaroshuk, Yongling Ai, Hao Chen
Peptide/protein quantitation using mass spectrometry (MS) is advantageous due to its high sensitivity. Traditional absolute peptide quantitation methods rely on making calibration curves using peptide standards or isotope-labelled peptide standards, which are expensive and take time to synthesize. A method which can eliminate the need for using standards would be beneficial. Recently, we developed coulometric mass spectrometry (CMS) which can be used to quantify peptides that are oxidizable (e.g., those containing tyrosine or tryptophan), without using peptide standard...
January 2024: International Journal of Mass Spectrometry
https://read.qxmd.com/read/37981917/triboelectric-nanogenerator-coated-blade-spray-mass-spectrometry-for-volume-limited-drug-analysis
#4
JOURNAL ARTICLE
Xin Ma, Facundo M Fernández
The demand for analytical tools for the analysis of low-concentration volume-limited samples has driven researchers to explore new analytical approaches. Mass spectrometry excels at trace analysis due to its high sensitivity and specificity, whereas ambient methods simplify, or completely eliminate sample preparation. Herein, we report a triboelectric nanogenerator-coated blade spray ambient mass spectrometry (TENG-CBS MS) method for the extraction, elution, and ionization of volume-limited, low-concentration small molecule drug samples with minimum sample preparation...
January 2024: International Journal of Mass Spectrometry
https://read.qxmd.com/read/37928050/towards-im-n-with-electrostatic-drift-fields-resetting-the-potential-of-trapped-ions-between-dimensions-of-ion-mobility
#5
JOURNAL ARTICLE
Benjamin P Zercher, Yuan Feng, Matthew F Bush
Increasing the dimensionality of ion mobility (IM) presents an enticing opportunity to increase the information content and selectivity of many analyses. However, for implementations of IM that use constant electrostatic gradients to separate ions in a buffer gas, technical challenges have limited the adoption of the technique and number of dimensions within individual experiments. Here, we introduce a strategy to "reset" the potentials of ions between IM dimensions. To achieve this, mobility-selected ions are trapped between dimensions of IM, using a combination of RF and electrostatic fields, while the subsequent dimension of IM is devoid of any drift field...
January 2024: International Journal of Mass Spectrometry
https://read.qxmd.com/read/38465269/maldi-peptide-mapping-for-fast-analysis-in-protein-footprinting
#6
JOURNAL ARTICLE
Ruidong Jiang, Don L Rempel, Michael L Gross
Although protein footprinting results are commonly obtained by ESI-based LC-MS/MS, a more rapid-turnaround alternative approach is desirable to expand the scope of protein footprinting and facilitate routine analysis such as monitoring protein high order structure in quality control or checking epitope maps. Considering that MALDI is a faster procedure that can be easily adapted for high-throughput analysis, we explore here the feasibility of developing a MALDI-based analysis "portfolio" of bottom-up peptide mass mapping for footprinting...
August 2023: International Journal of Mass Spectrometry
https://read.qxmd.com/read/37601139/structural-elucidation-and-relative-quantification-of-sodium-and-potassium-cationized-phosphatidylcholine-regioisomers-directly-from-tissue-using-electron-induced-dissociation
#7
JOURNAL ARTICLE
Tingting Yan, Matthias-Erich N Born, Boone M Prentice
Comprehensive structural characterization of phosphatidylcholines (PCs) is essential to understanding their biological functions and roles in metabolism. Electron induced dissociation (EID) of protonated PCs directly generated from biological tissues has previously been shown to provide in-depth structural information on the lipid headgroup, regiosiomerism of fatty acyl tails and double bond positions. Although phosphatidylcholine ions formed via alkali metal cationization ( i.e., [M + Na]+ and [M + K]+ ) are commonly generated during matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry experiments, the gas-phase ion chemistry behavior of EID on sodium- and potassium-cationized phosphatidylcholine ion types has not been studied for ions generated directly from tissue...
March 2023: International Journal of Mass Spectrometry
https://read.qxmd.com/read/36440090/assessing-the-use-of-host-guest-chemistry-in-conjunction-with-cyclic-ion-mobility-separations-for-the-linkage-specific-characterization-of-human-milk-oligosaccharides
#8
JOURNAL ARTICLE
Sanaz C Habibi, Gabe Nagy
Human milk oligosaccharides (HMOs) are a class of glycans that are highly abundant in human milk and contribute to the healthy growth of an infant's immune system. While new advancements in analytical methodologies have been made in glycomics, the high degree of isomeric heterogeneity and lack of authentic standards have made the high-resolution separation and accurate characterization of linkage positioning of all HMO species very challenging. Herein, we present an evaluation of the use of host-guest chemistry in conjunction with cyclic ion mobility spectrometry-mass spectrometry (cIMS-MS)-based separations for the identification of linkage positioning in three pairs of di-, tetra-, and hexasaccharide HMO isomers that only differ in the positioning of one glycosidic linkage (β1,3 versus β1,4)...
January 2023: International Journal of Mass Spectrometry
https://read.qxmd.com/read/36211475/separation-methods-in-single-cell-proteomics-rplc-or-ce
#9
JOURNAL ARTICLE
Kellye A Cupp-Sutton, Mulin Fang, Si Wu
Cellular heterogeneity is commonly investigated using single-cell genomics and transcriptomics to investigate biological questions such as disease mechanism, therapeutic screening, and genomic and transcriptomic diversity between cellular populations and subpopulations at the cellular level. Single-cell mass spectrometry (MS)-based proteomics enables the high-throughput examination of protein expression at the single-cell level with wide applicability, and with spatial and temporal resolution, applicable to the study of cellular development, disease, effect of treatment, etc...
November 2022: International Journal of Mass Spectrometry
https://read.qxmd.com/read/37577146/characterization-of-lipid-carbon-carbon-double-bond-isomerism-via-ion-mobility-mass-spectrometry-ims-ms-combined-with-cuprous-ion-induced-fragmentation
#10
JOURNAL ARTICLE
Syuan-Ting Kuo, Shuli Tang, David H Russell, Xin Yan
Characterization of phospholipid isomers is challenging due to their identical masses and similarities in structures. Here, we report that copper (I) ion complexed with phospholipids can be used to characterize both carbon-carbon double-bond (C=C bond) positional and geometric isomers. We investigate the distinct fragmentation patterns induced by the π-Cu+ interaction and developed strategies to rapidly characterize the isomerism of phospholipids. The multi-stage fragmentation of Cu+ -adducted lipids by collision-induced dissociation can generate diagnostic ions to locate C=C bonds in unsaturated lipids...
September 2022: International Journal of Mass Spectrometry
https://read.qxmd.com/read/37032994/adaptation-and-operation-of-a-quadrupole-time-of-flight-tandem-mass-spectrometer-for-high-mass-ion-ion-reaction-studies
#11
JOURNAL ARTICLE
Jay S Bhanot, Kimberly C Fabijanczuk, Abdirahman M Abdillahi, Hsi-Chun Chao, Nicolas J Pizzala, Frank A Londry, Eric T Dziekonski, James W Hager, Scott A McLuckey
A commercial quadrupole/time-of-flight tandem mass spectrometer has been modified and evaluated for its performance in conducting ion/ion reaction studies involving high mass (>100 kDa) ions. Modifications include enabling the application of dipolar AC waveforms to opposing rods in three quadrupole arrays in the ion path. This modification allows for resonance excitation of ions to effect ion activation, selective ion isolation, and ion parking. The other set of opposing rods in each array is enabled for the application of dipolar DC voltages for the purpose of broad-band (non-selective) ion heating...
August 2022: International Journal of Mass Spectrometry
https://read.qxmd.com/read/35386843/desalting-paper-spay-mass-spectrometry-dps-ms-for-rapid-detection-of-glycans-and-glycoconjugates
#12
JOURNAL ARTICLE
Kai-Yuan Chiu, Qi Wang, Harsha P Gunawardena, Michael Held, Ahmed Faik, Hao Chen
The detection of glycans and glycoconjugates has gained increasing attention in biological fields. Traditional mass spectrometry (MS)-based methods for glycoconjugate analysis are challenged with poor intensity when dealing with complex biological samples. We developed a desalting paper spray mass spectrometry (DPS-MS) strategy to overcome the issue of signal suppression of carbohydrates in salted buffer. Glycans and glycoconjugates (i.e., glycopeptides, nucleotide sugars, etc .) in non-volatile buffer (e.g...
November 2021: International Journal of Mass Spectrometry
https://read.qxmd.com/read/34744497/toward-improvement-of-screening-through-mass-spectrometry-based-proteomics-ovarian-cancer-as-a-case-study
#13
JOURNAL ARTICLE
Gordon T Luu, Laura M Sanchez
Ovarian cancer is one of the leading causes of cancer related deaths affecting United States women. Early-stage detection of ovarian cancer has been linked to increased survival, however, current screening methods, such as biomarker testing, have proven to be ineffective in doing so. Therefore, further developments are necessary to be able to achieve positive patient prognosis. Ongoing efforts are being made in biomarker discovery towards clinical applications in screening for early-stage ovarian cancer. In this perspective, we discuss and provide examples for several workflows employing mass spectrometry-based proteomics towards protein biomarker discovery and characterization in the context of ovarian cancer; workflows include protein identification and characterization as well as intact protein profiling...
November 2021: International Journal of Mass Spectrometry
https://read.qxmd.com/read/34421332/lipid-head-group-adduction-to-soluble-proteins-follows-gas-phase-basicity-predictions-dissociation-barriers-and-charge-abstraction
#14
JOURNAL ARTICLE
Micah T Donor, Jesse W Wilson, Samantha O Shepherd, James S Prell
Native mass spectrometry analysis of membrane proteins has yielded many useful insights in recent years with respect to membrane protein-lipid interactions, including identifying specific interactions and even measuring binding affinities based on observed abundances of lipid-bound ions after collision-induced dissociation (CID). However, the behavior of non-covalent complexes subjected to extensive CID can in principle be affected by numerous factors related to gas-phase chemistry, including gas-phase basicity (GB) and acidity, shared-proton bonds, and other factors...
November 2021: International Journal of Mass Spectrometry
https://read.qxmd.com/read/34539228/reassembling-protein-complexes-after-controlled-disassembly-by-top-down-mass-spectrometry-in-native-mode
#15
JOURNAL ARTICLE
Luis F Schachner, Denise P Tran, Alexander Lee, John P McGee, Kevin Jooss, Kenneth Durbin, Henrique Dos Santos Seckler, Lauren Adams, Erika Cline, Rafael Melani, Ashley N Ives, Benjamin Des Soye, Neil L Kelleher, Steven M Patrie
The combined use of electrospray ionization run in so-called "native mode" with top-down mass spectrometry (nTDMS) is enhancing both structural biology and discovery proteomics by providing three levels of information in a single experiment: the intact mass of a protein or complex, the masses of its subunits and non-covalent cofactors, and fragment ion masses from direct dissociation of subunits that capture the primary sequence and combinations of diverse post-translational modifications (PTMs). While intact mass data are readily deconvoluted using well-known software options, the analysis of fragmentation data that result from a tandem MS experiment - essential for proteoform characterization - is not yet standardized...
July 2021: International Journal of Mass Spectrometry
https://read.qxmd.com/read/33897275/application-of-frequency-multiple-ft-icr-ms-signal-acquisition-for-improved-proteome-research
#16
JOURNAL ARTICLE
Sung-Gun Park, Jared P Mohr, Gordon A Anderson, James E Bruce
Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) coupled with liquid chromatography (LC) is a powerful combination useful in many research areas due to the utility of high mass resolving power and mass measurement accuracy for studying highly complex samples. Ideally, every analyte in a complex sample can be subjected to accurate mass MS/MS analysis to aid in identification. FT-ICR MS can provide high mass resolving power and mass accuracy at the cost of long data acquisition periods, reducing the number of spectra that can be acquired per unit time...
July 2021: International Journal of Mass Spectrometry
https://read.qxmd.com/read/33716558/gas-phase-ion-ion-chemistry-for-structurally-sensitive-probes-of-gaseous-protein-ion-structure-electrostatic-and-electrostatic-to-covalent-cross-linking
#17
JOURNAL ARTICLE
Melanie Cheung See Kit, Veronica V Carvalho, Jonah Z Vilseck, Ian K Webb
Intramolecular interactions within a protein are key in maintaining protein tertiary structure and understanding how proteins function. Ion mobility-mass spectrometry (IM-MS) has become a widely used approach in structural biology since it provides rapid measurements of collision cross sections (CCS), which inform on the gas-phase conformation of the biomolecule under study. Gas-phase ion/ion reactions target amino acid residues with specific chemical properties and the modified sites can be identified by MS...
May 2021: International Journal of Mass Spectrometry
https://read.qxmd.com/read/33692650/towards-better-understanding-of-the-heparin-role-in-netosis-feasibility-of-using-native-mass-spectrometry-to-monitor-interactions-of-neutrophil-elastase-with-heparin-oligomers
#18
JOURNAL ARTICLE
Chendi Niu, Yi Du, Igor A Kaltashov
Neutrophil elastase is a serine protease released by neutrophils, and its dysregulation has been associated with a variety of debilitating pathologies, most notably cystic fibrosis. This protein is also a prominent component of the so-called neutrophil extracellular traps (NETs), whose formation is a part of the innate immunity response to invading pathogens, but also contributes to a variety of pathologies ranging from autoimmune disorders and inflammation to cancer to thrombotic complications in COVID-19...
May 2021: International Journal of Mass Spectrometry
https://read.qxmd.com/read/33679212/quantitative-assessment-of-six-different-reagent-gases-for-charge-transfer-dissociation-ctd-of-biological-ions
#19
JOURNAL ARTICLE
Zachary J Sasiene, Praneeth M Mendis, Glen P Jackson
Charge transfer dissociation mass spectrometry (CTD-MS) has been shown to induce high energy fragmentation of biological ions in the gas phase and provide fragmentation spectra similar to extreme ultraviolet photodissociation (XUVPD). To date, CTD has typically employed helium cations with kinetic energies between 4-10 keV to initiate radical-directed fragmentation of analytes. However, as a reagent, helium has recently been listed as a critical mineral that is becoming scarcer and more expensive, so this study explored the potential for using cheaper and more readily available reagent gases...
April 2021: International Journal of Mass Spectrometry
https://read.qxmd.com/read/33642939/investigating-native-capillary-zone-electrophoresis-mass-spectrometry-on-a-high-end-quadrupole-time-of-flight-mass-spectrometer-for-the-characterization-of-monoclonal-antibodies
#20
JOURNAL ARTICLE
Xiaojing Shen, Zhijie Liang, Tian Xu, Zhichang Yang, Qianjie Wang, Daoyang Chen, Lucynda Pham, Wenjun Du, Liangliang Sun
Native capillary zone electrophoresis-mass spectrometry (CZE-MS) has attracted attentions for the characterization of monoclonal antibodies (mAbs) due to the potential of CZE for highly efficient separations of mAbs under native conditions as well as its compatibility with native electrospray ionization (ESI)-MS. However, the low sample loading capacity and limited separation resolution of native CZE for large proteins and protein complexes ( e.g. mAbs) impede the widespread adoption of native CZE-MS. Here, we present a novel native capillary isoelectric focusing (cIEF)-assisted CZE-MS method for the characterization of mAbs with much larger sample loading capacity and significantly better separation resolution than native CZE-MS alone...
April 2021: International Journal of Mass Spectrometry
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