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Analytical and Bioanalytical Chemistry

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https://www.readbyqxmd.com/read/28735452/characterization-of-polymeric-substance-classes-in-cereal-based-beverages-using-asymmetrical-flow-field-flow-fractionation-with-a-multi-detection-system
#1
Georg Krebs, Thomas Becker, Martina Gastl
Cereal-based beverages contain a complex mixture of various polymeric macromolecules including polysaccharides, peptides, and polyphenols. The molar mass of polymers and their degradation products affect different technological and especially sensory parameters of beverages. Asymmetrical flow field-flow fractionation (AF4) coupled with multi-angle light scattering (MALS) and refractive index detection (dRI) or UV detection (UV) is a technique for structure and molar mass distribution analysis of macromolecules commonly used for pure compound solutions...
July 22, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28735451/importance-of-icpms-for-speciation-analysis-is-changing-future-trends-for-targeted-and-non-targeted-element-speciation-analysis
#2
Joerg Feldmann, Andrea Raab, Eva M Krupp
This article is aimed at researchers interested in organic molecules which contain a heteroatom but who have never considered using inductively coupled plasma mass spectrometry (ICPMS) or who have used ICPMS for years and developed numerous methods for analysis of target elemental species. We try to illustrate (1) that ICPMS has been very useful for speciation analysis of metal(loid) target species and that there is now a trend to replace the costly detector with cheaper detection systems for routine target analysis, and (2) that ICPMS has been used and will be used even more in the future for non-targeted analysis of elements which are not normally associated with ICPMS analysis, such as non-metals such as sulfur, phosphorus, chlorine and fluorine...
July 22, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730313/derivatization-free-lc-ms-ms-method-for-estrogen-quantification-in-mouse-brain-highlights-a-local-metabolic-regulation-after-oral-versus-subcutaneous-administration
#3
Ecaterina Lozan, Svitlana Shinkaruk, Shaam Alice Al Abed, Valérie Lamothe, Mylène Potier, Aline Marighetto, Jean-Marie Schmitter, Catherine Bennetau-Pelissero, Corinne Buré
17β-Estradiol (17β-E2) is a steroid with pleiotropic actions. In addition to being a sexual hormone, it is also produced in the brain where it modulates the reproductive axis. It has been shown that 17β-E2 also acts on synaptic plasticity and plays a role in neurological pathways and in neurodegenerative diseases. Assaying this steroid in the brain is thus interesting to improve our knowledge of 17β-E2 effects in the brain. However, 17β-E2 concentration in the central nervous system has been reported to be of a few nanograms per gram wet weight (nanomolar range concentration); therefore, its quantification requires both an efficient extraction process and a sensitive detection method...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730312/hydrophilic-interaction-liquid-chromatography-tandem-mass-spectrometry-for-quantitation-of-paralytic-shellfish-toxins-validation-and-application-to-reference-materials
#4
Krista M Thomas, Daniel G Beach, Kelley L Reeves, Ryan S Gibbs, Elliott S Kerrin, Pearse McCarron, Michael A Quilliam
Paralytic shellfish toxins (PSTs) are potent neurotoxins produced by marine dinoflagellates that are responsible for paralytic shellfish poisoning (PSP) in humans. This work highlights our ongoing efforts to develop quantitative methods for PSTs using hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). Compared with the commonly used method of liquid chromatography with post-column oxidation and fluorescence detection (LC-ox-FLD), HILIC-MS/MS has the potential of being more robust, sensitive and straightforward to operate, and provides unequivocal confirmation of toxin identity...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730311/aptamer-based-fluorescence-quenching-lateral-flow-strip-for-rapid-detection-of-mercury-ii-ion-in-water-samples
#5
Ze Wu, Haicong Shen, Junhui Hu, Qiangqiang Fu, Cuize Yao, Shiting Yu, Wei Xiao, Yong Tang
Divalent mercury ion (Hg(2+)) is one of the most common and stable forms of mercury pollution. In this study, a skillfully designed lateral flow strip (LFS) was developed for sensitive detection of Hg(2+) in river water samples. Aptamer, a specific oligonucleotide probe, was used to selectively identify and target Hg(2+) instead of antibody in traditional immunechromatographic strips; and the fluorescence-quenching system was used to generate positive and low background florescence signals in the competitive-likely LFS...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730310/optical-biosensor-optimized-for-continuous-in-line-glucose-monitoring-in-animal-cell-culture
#6
Mircea Tric, Mario Lederle, Lisa Neuner, Igor Dolgowjasow, Philipp Wiedemann, Stefan Wölfl, Tobias Werner
Biosensors for continuous glucose monitoring in bioreactors could provide a valuable tool for optimizing culture conditions in biotechnological applications. We have developed an optical biosensor for long-term continuous glucose monitoring and demonstrated a tight glucose level control during cell culture in disposable bioreactors. The in-line sensor is based on a commercially available oxygen sensor that is coated with cross-linked glucose oxidase (GOD). The dynamic range of the sensor was tuned by a hydrophilic perforated diffusion membrane with an optimized permeability for glucose and oxygen...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730309/a-simple-and-rapid-infrared-assisted-self-enzymolysis-extraction-method-for-total-flavonoid-aglycones-extraction-from-scutellariae-radix-and-mechanism-exploration
#7
Liping Wang, Haotian Duan, Jiebing Jiang, Jiakun Long, Yingjia Yu, Guiliang Chen, Gengli Duan
A new, simple, and fast infrared-assisted self enzymolysis extraction (IRASEE) approach for the extraction of total flavonoid aglycones (TFA) mainly including baicalein, wogonin, and oroxylin A from Scutellariae Radix is presented to enhance extraction yield. Extraction enzymolysis temperature, enzymolysis liquid-to-solid ratio, enzymolysis pH, enzymolysis time and infrared power, the factors affecting IRASEE procedure, were investigated in a newly designed, temperature-controlled infrared-assisted extraction (TC-IRAE) system to acquire the optimum analysis conditions...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730308/tlc-surface-integrity-affects-the-detection-of-alkali-adduct-ions-in-tlc-maldi-analysis
#8
Yonghui Dong, Ruggero Ferrazza, Andrea Anesi, Graziano Guella, Pietro Franceschi
Direct coupling of thin-layer chromatography (TLC) with matrix-assisted laser desorption ionization (MALDI) mass spectrometry allows fast and detailed characterization of a large variety of analytes. The use of this technique, however, presents great challenges in semiquantitative applications because of the complex phenomena occurring at the TLC surface. In our laboratory, we recently observed that the ion intensities of several alkali adduct ions were significantly different between the top and interior layer of the TLC plate...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730307/experiences-and-benefits-of-a-career-development-course-for-undergraduate-chemistry-students
#9
Charles A Lucy
No abstract text is available yet for this article.
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730306/disposable-electrochemical-immunosensor-for-brettanomyces-bruxellensis-based-on-nanogold-reduced-graphene-oxide-hybrid-nanomaterial
#10
Boryana Borisova, María L Villalonga, María Arévalo-Villena, Abderrahmane Boujakhrout, Alfredo Sánchez, Concepción Parrado, José M Pingarrón, Ana Briones-Pérez, Reynaldo Villalonga
The assembly of a novel disposable amperometric immunosensor for the detection of the red wine spoilage yeast Brettanomyces bruxellensis is reported. The nanostructured sensing interface was prepared by first coating carbon screen printed electrodes with a gold nanoparticles-reduced graphene oxide hybrid nanomaterial, which was then modified with 3-mercaptopropionic acid to further immobilize specific antibodies for B. bruxellensis via a carbodiimide-coupling reaction. The functionalized electrode allowed the amperometric detection of B...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730305/self-assembly-of-the-polymer-brush-grafted-silica-colloidal%C3%A2-array-for-recognition-of-proteins
#11
Wei Chen, Kenneth J Shea, Min Xue, Lili Qiu, Yunhe Lan, Zihui Meng
Three-dimensional photonic crystal sensors are attractive platforms for autonomous chemical sensing and colorimetric bioassays. At present, the photonic crystal sensors with inverse opal structure were extensively studied, which swells or shrinks in response to the analytes. However, the fabrication of inverse opal sensors still remains a major challenge. Herein, we propose a simple and versatile approach to fabricate 3D opal photonic sensors. This photonic crystal is fabricated via assembly of monodispersed silica particles grafted with linear polymeric ligands (SiO2@LPs)...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730304/novel-ribonuclease-activity-of-cusativin-from-cucumis-sativus-for-mapping-nucleoside-modifications-in-rna
#12
Balasubrahmanyam Addepalli, Sarah Venus, Priti Thakur, Patrick A Limbach
A recombinant ribonuclease, cusativin, was characterized for its cytidine-specific cleavage ability of RNA to map chemical modifications. Following purification of native cusativin protein as described before (Rojo et al. Planta 194:328, 17), partial amino acid sequencing was carried out to identify the corresponding protein coding gene in cucumber genome. Cloning and heterologous expression of the identified gene in Escherichia coli resulted in successful production of active protein as a C-terminal His-tag fusion protein...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730303/exhaled-breath-online-measurement-for-cervical-cancer-patients-and-healthy-subjects-by-proton-transfer-reaction-mass-spectrometry
#13
Wenzhao Zhou, Chaoqun Huang, Xue Zou, Yan Lu, Chengyin Shen, Xiping Ding, Hongzhi Wang, Haihe Jiang, Yannan Chu
Cervical cancer is a common cancer among women and has a high morbidity and mortality. The traditional clinical methods for cervical cancer screening are invasive and limited in terms of cost and time. There is an unmet clinical need for new methods to aid clinicians in the rapid screening and auxiliary diagnosis of cervical precancer. Recently, breath analysis has become an attractive approach for investigation of cancer biomarkers and shows great potential in cancer screening owing to its high sensitivity, quickness, and non-invasive nature...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28717898/thiophene-based-rhodamine-as-selectivef-luorescence-probe-for-fe-iii-and-al-iii-in-living-cells
#14
Kun-Peng Wang, Ju-Peng Chen, Si-Jie Zhang, Yang Lei, Hua Zhong, Shaojin Chen, Xin-Hong Zhou, Zhi-Qiang Hu
The thiophene-modified rhodamine 6G (GYJ) has been synthesized as a novel chemosensor. The sensor has sufficiently high selectivity and sensitivity for the detection of Fe(3+) and Al(3+) ions (M(3+)) by fluorescence and ultraviolet spectroscopy with a strong ability for anti-interference performance. The binding ratio of M(3+)-GYJ complex was determined to be 2:1 according to the Job's plot. The binding constants for Fe(3+) and Al(3+) were calculated to be 3.91 × 10(8) and 5.26 × 10(8) M(-2), respectively...
July 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28717897/development-of-a-fast-and-cost-effective-gas-chromatography-mass-spectrometry-method-for-the-quantification-of-short-chain-and-medium-chain-fatty-acids-in-human-biofluids
#15
Cesare Lotti, Josep Rubert, Francesca Fava, Kieran Tuohy, Fulvio Mattivi, Urska Vrhovsek
The quantification of short-chain and medium-chain fatty acids is becoming more and more relevant in fecal and plasma samples due to their biological impact, which has been associated with colon rectal cancer and fiber consumption. For these reasons, a fast, cost-effective, and reproducible analytical method is highly required. In this research, a gas chromatography-mass spectrometry method based on full scan and multiple reaction monitoring (MRM) acquisition modes were optimized and validated for the analysis of short-chain and medium-chain fatty acids in three biological samples: human fecal water, fecal fermentation supernatants, and human plasma...
July 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28717896/optimization-of-lipid-extraction-and-analytical-protocols-for-uhplc-esi-hrms-based-lipidomic-analysis-of-adherent-mammalian-cancer-cells
#16
Huizhen Zhang, Yue Gao, Jiahong Sun, Shicheng Fan, Xinpeng Yao, Xiaorong Ran, Chuanqi Zheng, Min Huang, Huichang Bi
Lipidomics, which reveals comprehensive characterization of molecular lipids, is a rapidly growing technology used in biomedical research. Lipid extraction is a critical step in lipidomic analysis. However, the effectiveness of different lipid extract solvent systems from cellular samples still remains unclear. In the current study, the protocol of reverse-phase liquid chromatography mass spectrometry (LC/MS)-based lipidomics was optimized for extraction and detection of lipids from human pancreatic cancer cell line PANC-1...
July 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28717895/correlating-uptake-and-activity-of-proline-rich-antimicrobial-peptides-in-escherichia-coli
#17
Luzia Holfeld, Ralf Hoffmann, Daniel Knappe
Increasing death tolls accounted for by antimicrobial drug resistance demand novel antibiotic lead compounds. Among different promising candidate classes, proline-rich antimicrobial peptides (PrAMPs) are very favorable due to their intracellular mechanism, i.e., binding to the 70S ribosome and DnaK, after active uptake relying on bacterial transporters like SbmA and MdtM. Studies on peptide internalization as the first step of their complex mode of action rely typically on fluorophore or radioactive labeling and quantification using microscopy, flow cytometry, or radioactivity...
July 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28717894/determination-of-ketones-and-ethyl-acetate-a-preliminary-study-for-the-discrimination-of-patients-with-lung-cancer
#18
Patricia Martín Santos, Miguel Del Nogal Sánchez, Ángel Pedro Crisolino Pozas, José Luis Pérez Pavón, Bernardo Moreno Cordero
In this work, ten possible volatile biomarkers of lung cancer (acetone, 2-butanone, ethyl acetate, 2-pentanone, 4-methyl-2-pentanone, 2-hexanone, 3-heptanone, 2-heptanone, 3-octanone, and 2-nonanone) have been analyzed to evaluate their different concentration levels in urine samples from lung cancer patients (n = 12) and healthy controls (n = 12). The volatile compounds were generated with a headspace autosampler and analyzed with a gas chromatograph equipped with a programmed temperature vaporizer and mass spectrometry detector (HS-PTV-GC-MS)...
July 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28717893/normal-phase-liquid-chromatography-retention-behavior-of-polycyclic-aromatic-hydrocarbon-and-their-methyl-substituted-derivatives-on-an-aminopropyl-stationary-phase
#19
Walter B Wilson, Hugh V Hayes, Lane C Sander, Andres D Campiglia, Stephen A Wise
Retention indices for 124 polycyclic aromatic hydrocarbons (PAHs) and 62 methyl-substituted (Me-) PAHs were determined using normal-phase liquid chromatography (NPLC) on a aminopropyl (NH2) stationary phase. PAH retention behavior on the NH2 phase is correlated to the total number of aromatic carbons in the PAH structure. Within an isomer group, non-planar isomers generally elute earlier than planar isomers. MePAHs generally elute slightly later but in the same region as the parent PAHs. Correlations between PAH retention behavior on the NH2 phase and PAH thickness (T) values were investigated to determine the influence of non-planarity for isomeric PAHs with four to seven aromatic rings...
July 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28714042/magnetic-nanoparticles-carbon-nanotubes-hybrid-composites-for-selective-solid-phase-extraction-of-polycyclic-aromatic-hydrocarbons-and-determination-by-ultra-high-performance-liquid-chromatography
#20
Ana I Corps Ricardo, Francisco J Guzmán Bernardo, Mohammed Zougagh, Rosa C Rodríguez Martín-Doimeadios, Ángel Ríos
A rapid and reliable method based on magnetic solid-phase extraction (MSPE) and ultra-high performance liquid chromatography (UHPLC-FLD) analysis was developed and validated for the quantitative determination of seven polycyclic aromatic hydrocarbons (PAHs) in water samples. Hybrid composites made up of magnetic nanoparticles (MNPs) and carbon nanotubes (CNTs) were tested as adsorbent materials. The main factors influencing the extraction yields were optimized, namely the amount and type of adsorbent in the adsorption, and the solvent, the type of energy and the time in the desorption step...
July 17, 2017: Analytical and Bioanalytical Chemistry
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