Ying Wang, Li Li, Adam T Sutton, Qiang Tu, Kaixi Zhao, Emily Wen, James Osborn, Andrew Singh, Matthew J Gunsch, Richard Rianto Rustandi, David Foley, Yu He
Messenger RNA (mRNA) vaccines represent a landmark in vaccinology, especially with their success in COVID-19 vaccines, which have shown great promise for future vaccine development and disease prevention. As a platform technology, synthetic mRNA can be produced with high fidelity using in vitro transcription (IVT). Magnesium plays a vital role in the IVT process, facilitating the phosphodiester bond formation between adjacent nucleotides and ensuring accurate transcription to produce high-quality mRNA. The development of the IVT process has prompted key inquiries about in-process characterization of magnesium ion (Mg++ ) consumption, relating to the RNA polymerase (RNAP) activation, fed-batch mode production yield, and mRNA quality...
April 10, 2024: Analytical and Bioanalytical Chemistry