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Analytical and Bioanalytical Chemistry

Muhsin Aydin, Jacqueline Carter-Conger, Ning Gao, David F Gilmore, Steven C Ricke, Soohyoun Ahn
Salmonella is one of major foodborne pathogens and the leading cause of foodborne illness-related hospitalizations and deaths. It is critical to develop a sensitive and rapid detection assay that can identify Salmonella to ensure food safety. In this study, a DNA sensor-based suspension array system of high multiplexing ability was developed to identify eight Salmonella serovars commonly associated with foodborne outbreaks to the serotype level. Each DNA sensor was prepared by activating pre-encoded microspheres with oligonucleotide probes that are targeting virulence genes and serovar-specific regions...
February 19, 2018: Analytical and Bioanalytical Chemistry
Paolo Ugo
No abstract text is available yet for this article.
February 19, 2018: Analytical and Bioanalytical Chemistry
Siriluck Pojjanapornpun, Yada Nolvachai, Kornkanok Aryusuk, Chadin Kulsing, Kanit Krisnangkura, Philip J Marriott
New generation inert ionic liquid (iIL) GC columns IL60i, IL76i and IL111i, comprising phosphonium or imidazolium cationic species, were investigated for separation of fatty acid methyl esters (FAME). In general, the iIL phases provide comparable retention times to their corresponding conventional columns, with only minor selectivity differences. The average tailing factors and peak widths were noticeably improved (reduced) for IL60i and IL76i, while they were slightly improved for IL111i. Inert IL phase columns were coupled with conventional IL columns in comprehensive two-dimensional GC (GC × GC) with a solid-state modulator which offers variable modulation temperature (TM ), programmable TM during analysis and trapping stationary phase material during the trap/release (modulation) process, independent of oven T and column sets...
February 17, 2018: Analytical and Bioanalytical Chemistry
Tessema F Mekonnen, Ulrich Panne, Matthias Koch
Biotransformation processes of fluopyram (FLP), a new succinate dehydrogenase inhibitor (SDHI) fungicide, were investigated by electrochemistry (EC) coupled online to liquid chromatography (LC) and electrospray mass spectrometry (ESI-MS). Oxidative phase I metabolite production was achieved using an electrochemical flow-through cell equipped with a boron-doped diamond (BDD) electrode. Structural elucidation and prediction of oxidative metabolism pathways were assured by retention time, isotopic patterns, fragmentation, and accurate mass measurements using EC/LC/MS, LC-MS/MS, and/or high-resolution mass spectrometry (HRMS)...
February 17, 2018: Analytical and Bioanalytical Chemistry
Måns Ekelöf, Jeffrey Manni, Milad Nazari, Mark Bokhart, David C Muddiman
Laser systems are widely used in mass spectrometry as sample probes and ionization sources. Mid-infrared lasers are particularly suitable for analysis of high water content samples such as animal and plant tissues, using water as a resonantly excited sacrificial matrix. Commercially available mid-IR lasers have historically been bulky and expensive due to cooling requirements. This work presents a novel air-cooled miniature mid-IR laser with adjustable burst-mode output and details an evaluation of its performance for mass spectrometry imaging...
February 17, 2018: Analytical and Bioanalytical Chemistry
Mesay Mulugeta Wolle, Sean D Conklin
Several extraction and chromatographic methods were evaluated to identify optimum conditions for arsenic speciation analysis in seafood and seaweed. The extraction systems, which include aqueous, aqueous-organic, acidic, basic, and enzymatic solutions, were examined for their efficiency in extracting arsenic from finfish, crustaceans, molluscs, and seaweed keeping the chemical forms of the native arsenicals intact. While dilute solutions of nitric acid, hydrochloric acid, and tetramethylammonium hydroxide (TMAH) extract high fractions of arsenic from most of the matrices, the extractants oxidized arsenite (As3+ ) to arsenate (As5+ ) and converted some arsenosugars and non-polar arsenicals to known and/or unknown forms...
February 17, 2018: Analytical and Bioanalytical Chemistry
Minghao Ge, Pengli Bai, Mingli Chen, Jingjing Tian, Jun Hu, Xu Zhi, Huancai Yin, Jian Yin
Here, we utilized the ultrasonic emulsification technique to generate hyaluronic acid microspheres incorporating a fluorescence-based glucose biosensor. We synthesized a novel lanthanide ion luminophore based on Eu3+ . Eu sulfosuccinimidyl dextran (Eu-dextran) and Alexa Fluor 647 sulfosuccinimidyl-ConA (Alexa Fluor 647-ConA) were encapsulated in hyaluronic acid hydrogel to generate microspheres. Glucose sensing was carried out using a fluorescence resonance energy transfer (FRET)-based assay principle. A proportional fluorescence intensity increase was found within a 0...
February 17, 2018: Analytical and Bioanalytical Chemistry
Ivona Lhotská, Barbora Gajdošová, Petr Solich, Dalibor Šatínský
Sample preparation prior to chromatographic separation plays an important role in the analytical process. To avoid time-consuming and manual handling sample-prep, automated on-line techniques such as on-line SPE-HPLC are therefore preferred. In this study, two different on-line extraction approaches for mycotoxin/endocrine disruptor zearalenone (ZEA) determination using either molecularly imprinted polymer (MIP) with selective cavities and binding sites for extraction or a reversed-phase sorbent C18 providing non-selective interactions have been developed, validated, and compared...
February 17, 2018: Analytical and Bioanalytical Chemistry
Xu Wang, Fang Li, Ziqi Cai, Kaifan Liu, Jing Li, Boyang Zhang, Jianbo He
In this work, a multilayer-modified paper-based colorimetric sensing platform with improved color uniformity and intensity was developed for the sensitive and selective determination of uric acid and glucose with smartphone as signal readout. In detail, chitosan, different kinds of chromogenic reagents, and horseradish peroxidase (HRP) combined with a specific oxidase, e.g., uricase or glucose oxidase (GOD), were immoblized onto the paper substrate to form a multilayer-modified test paper. Hydrogen peroxide produced by the oxidases (uricase or GOD) reacts with the substrates (uric acid or glucose), and could oxidize the co-immoblized chromogenic reagents to form colored products with HRP as catalyst...
February 17, 2018: Analytical and Bioanalytical Chemistry
Joshua Beri, Kaylie I Kirkwood, David C Muddiman, Michael S Bereman
We describe a set of new tools for the detection and quantification of β-N-methylamino-L-alanine (BMAA) which includes a novel stable isotope-labeled BMAA standard (13 C3 ,15 N2 ) and a chip-based capillary electrophoresis mass spectrometry platform for separation and detection. Baseline resolution of BMAA from its potentially confounding structural isomers N-2-aminoethylglycine (AEG) and 2,4-diaminobutyric acid (2,4-DAB) is achieved using the chip-based CE-MS system in less than 1 min. Detection and linearity of response are demonstrated across > 3...
February 17, 2018: Analytical and Bioanalytical Chemistry
Sofie Velghe, Christophe P Stove
We would like to call the reader's attention to the fact that unfortunately in fig. 2 of the original article the figure headings of both graphs are the same.
February 15, 2018: Analytical and Bioanalytical Chemistry
Sherri B Turnipseed, Joseph M Storey, I-Lin Wu, Charles M Gieseker, Nicholas R Hasbrouck, Tina C Crosby, Wendy C Andersen, Shanae Lanier, Christine R Casey, Robert Burger, Mark R Madson
The ability to detect chemical contaminants, including veterinary drug residues in animal products such as fish, is an important example of food safety analysis. In this paper, a liquid chromatography high-resolution mass spectrometry (LC-HRMS) screening method using a quadrupole-Orbitrap instrument was applied to the analysis of veterinary drug residues in incurred tissues from aquacultured channel catfish, rainbow trout, and Atlantic salmon and imported aquacultured products including European eel, yellow croaker, and tilapia...
February 14, 2018: Analytical and Bioanalytical Chemistry
Jenny Schröter, Annabelle Fülöp, Carsten Hopf, Jürgen Schiller
Unequivocal assignment of phospholipid peaks in complex mixtures is difficult if only the m/z values but no tandem mass spectrometry (MS/MS) data are available. This is usually the case for matrix-assisted laser/desorption ionization time-of-flight (MALDI-TOF) MS imaging experiments and the analysis has normally to be performed without prior separation. Another problem might be the often matrix-induced loss of one methyl group in phosphatidylcholine (PC) species, which makes them detectable as negative ions becoming isomers of some phosphatidylethanolamines (PEs)...
February 14, 2018: Analytical and Bioanalytical Chemistry
Tingting Bai, Meng Wang, Min Cao, Juan Zhang, Kangzhen Zhang, Ping Zhou, Zhengxia Liu, Ying Liu, Zhirui Guo, Xiang Lu
Lateral flow assay strips (LFASs) with Au nanoparticles (NPs) have been widely used as a probe for biomarkers in point-of-care testing; however, there still remain challenges in detection sensitivity and quantitative analysis. In this study, we developed a surface-enhanced Raman scattering (SERS)-based LFAS for quantitative analysis of a biomarker in the low concentration range. Moreover, apart from conventional Au NPs, three other types of citrate-capped Au-Ag bimetallic NPs: Au core with Ag shell NPs (Au@Ag NPs), rattle-like Au core in Ag-Au shell NPs (Au@Ag-Au NPs) and Ag-Au NPs were prepared and functionalized, and their solution-based SERS activities were comprehensively studied by experimental measurement and theoretical analysis...
February 14, 2018: Analytical and Bioanalytical Chemistry
Mario Thevis, Oliver Krug, Hans Geyer, Katja Walpurgis, Norbert Baume, Andreas Thomas
Analytical chemistry represents a central aspect of doping controls. Routine sports drug testing approaches are primarily designed to address the question whether a prohibited substance is present in a doping control sample and whether prohibited methods (for example, blood transfusion or sample manipulation) have been conducted by an athlete. As some athletes have availed themselves of the substantial breadth of research and development in the pharmaceutical arena, proactive and preventive measures are required such as the early implementation of new drug candidates and corresponding metabolites into routine doping control assays, even though these drug candidates are to date not approved for human use...
February 14, 2018: Analytical and Bioanalytical Chemistry
Shuang-Shuang Chai, Mei-Ling Qin, You-Ning Ma, Huan-Huan Gao, Qiao He, Han-Tong Zhang
The authors would like to call the reader's attention to the fact that unfortunately during a recent cross-check of the experimental record, they found that the positions of intercept and slope were reversed in Table 1 in the original manuscript. The authors apologize for the mistake.
February 13, 2018: Analytical and Bioanalytical Chemistry
Nevena Maljurić, Jelena Golubović, Biljana Otašević, Mira Zečević, Ana Protić
Applying green chromatography methods is currently one of the challenges in liquid chromatography. Among different strategies, using cyclodextrin (CD) mobile phase modifiers was applied in this paper. CDs can form inclusion complexes with a wide variety of hydrophobic organic compounds and, consequently, affect their retention behavior. CD-containing mobile phases possess complicated complexation and adsorption equilibria so retention is dependent not only on chromatographic parameters and properties of the compound but also on properties of compound-CD complex...
February 13, 2018: Analytical and Bioanalytical Chemistry
Rosa Pilolli, Elisabetta De Angelis, Linda Monaci
In recent years, mass spectrometry (MS) has been establishing its role in the development of analytical methods for multiple allergen detection, but most analyses are being carried out on low-resolution mass spectrometers such as triple quadrupole or ion traps. In this investigation, performance provided by a high resolution (HR) hybrid quadrupole-Orbitrap™ MS platform for the multiple allergens detection in processed food matrix is presented. In particular, three different acquisition modes were compared: full-MS, targeted-selected ion monitoring with data-dependent fragmentation (t-SIM/dd2), and parallel reaction monitoring...
February 13, 2018: Analytical and Bioanalytical Chemistry
Cecilia Cagliero, Carlo Bicchi, Chiara Cordero, Erica Liberto, Patrizia Rubiolo, Barbara Sgorbini
Fragrances and products deriving from essential oils are often formulated or diluted in aqueous media, usually ethanol/water. Gas chromatography (GC) is the technique of choice to analyze volatiles. However, when using columns coated with conventional stationary phases, its application to aqueous samples often requires time-consuming and/or discriminative sample preparation techniques to extract the target analytes from the aqueous medium, so as to avoid its direct injection. In GC with conventional columns, water produces peak asymmetry, poor sensitivity and efficiency, strong adsorption, stationary phase degradation, and, last but not least, it is not easy to detect reliably when present in high amounts...
February 13, 2018: Analytical and Bioanalytical Chemistry
Bruce A Benner, Michele M Schantz, Carissa D Powers, Rosemary L Schleicher, Johanna E Camara, Katherine E Sharpless, James H Yen, Lorna T Sniegoski
Dietary fatty acids can be both beneficial and detrimental to human health depending on the degree and type of saturation. Healthcare providers and research scientists monitor the fatty acid content of human plasma and serum as an indicator of health status and diet. In addition, both the Centers for Disease Control & Prevention (CDC) and the National Institutes of Health - Office of Dietary Supplements are interested in circulating fatty acids (FAs) because they may be predictive of coronary heart disease...
February 13, 2018: Analytical and Bioanalytical Chemistry
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