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Analytical and Bioanalytical Chemistry

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https://www.readbyqxmd.com/read/28429066/atmospheric-pressure-maldi-for-the-noninvasive-characterization-of-carbonaceous-ink-from-renaissance-documents
#1
Giuseppe Grasso, Marzia Calcagno, Alessandro Rapisarda, Roberta D'Agata, Giuseppe Spoto
The analytical methods that are usually applied to determine the compositions of inks from ancient manuscripts usually focus on inorganic components, as in the case of iron gall ink. In this work, we describe the use of atmospheric pressure/matrix-assisted laser desorption ionization-mass spectrometry (AP/MALDI-MS) as a spatially resolved analytical technique for the study of the organic carbonaceous components of inks used in handwritten parts of ancient books for the first time. Large polycyclic aromatic hydrocarbons (L-PAH) were identified in situ in the ink of XVII century handwritten documents...
April 21, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28429065/rapid-electrochemical-quantification-of-salmonella-pullorum-and-salmonella-gallinarum-based-on-glucose-oxidase-and-antibody-modified-silica-nanoparticles
#2
Yiheng Luo, Wenchao Dou, Guangying Zhao
In this article, a facile and sensitive electrochemical method for quantification of Salmonella Pullorum and Salmonella Gallinarum (S. Pullorum and S. Gallinarum) was established by monitoring glucose consumption with a personal glucose meter (PGM). Antibody-functionalized magnetic nanoparticles (IgG-MNPs) were used to capture and enrich S. Pullorum and S. Gallinarum, and IgG-MNPs-S. Pullorum and IgG-MNPs-S. Gallinarum complexes were magnetically separated from a sample using a permanent magnet. The trace tag was prepared by loading polyclonal antibodies and high-content glucose oxidase on amino-functionalized silica nanoparticles (IgG-SiNPs-GOx)...
April 21, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28429064/simple-and-cost-effective-liquid-chromatography-mass-spectrometry-method-to-measure-dabrafenib-quantitatively-and-six-metabolites-semi-quantitatively-in-human-plasma
#3
Svante Vikingsson, Jan-Olof Dahlberg, Johan Hansson, Veronica Höiom, Henrik Gréen
Dabrafenib is an inhibitor of BRAF V600E used for treating metastatic melanoma but a majority of patients experience adverse effects. Methods to measure the levels of dabrafenib and major metabolites during treatment are needed to allow development of individualized dosing strategies to reduce the burden of such adverse events. In this study, an LC-MS/MS method capable of measuring dabrafenib quantitatively and six metabolites semi-quantitatively is presented. The method is fully validated with regard to dabrafenib in human plasma in the range 5-5000 ng/mL...
April 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28429063/direct-fluorescence-anisotropy-assay-for-cocaine-using-tetramethylrhodamine-labeled-aptamer
#4
Yingxiong Liu, Qiang Zhao
Development of simple, sensitive, and rapid method for cocaine detection is important in medicine and drug abuse monitoring. Taking advantage of fluorescence anisotropy and aptamer, this study reports a direct fluorescence anisotropy (FA) assay for cocaine by employing an aptamer probe with tetramethylrhodamine (TMR) labeled on a specific position. The binding of cocaine and the aptamer causes a structure change of the TMR-labeled aptamer, leading to changes of the interaction between labeled TMR and adjacent G bases in aptamer sequence, so FA of TMR varies with increasing of cocaine...
April 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28429062/detection-of-freshwater-cyanotoxins-and-measurement-of-masked-microcystins-in-tilapia-from-southeast-asian-aquaculture-farms
#5
Brett Greer, Ronald Maul, Katrina Campbell, Christopher T Elliott
Recently, there has been a rise in freshwater harmful algal blooms (HABs) globally, as well as increasing aquaculture practices. HABs can produce cyanotoxins, many of which are hepatotoxins. An ultra-performance liquid chromatography tandem mass spectrometry method was developed and validated for nine cyanotoxins across three classes including six microcystins, nodularin, cylindrospermopsin and anatoxin-a. The method was used to analyse free cyanotoxin(s) in muscle (n = 34), liver (n = 17) and egg (n = 9) tissue samples of 34 fish sourced from aquaculture farms in Southeast Asia...
April 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28424859/investigation-of-carbon-based-nanomaterials-as-sorbents-for-headspace-in-tube-extraction-of-polycyclic-aromatic-hydrocarbons
#6
Xochitli L Osorio Barajas, Thorsten Hüffer, Pascal Mettig, Beat Schilling, Maik A Jochmann, Torsten C Schmidt
Carbon-based nanomaterials (CNM) represent promising materials for the application as sorbents in micro- and other extraction devices. In this work, we investigate the applicability of five different CNM (multi-walled carbon nanotubes (MWCNTs), fullerenes, carboxylic acid functionalized multi-walled carbon nanotubes (MWCNTs-COOH), graphene platelets, and carbon nanohorns) for their performance on PAH extraction from the aqueous phase by headspace in-tube extraction (HS-ITEX). Optimal extraction parameters for HS-ITEX were determined using a Box-Behnken experimental design...
April 19, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28424858/electrochemical-simulation-of-biotransformation-reactions-of-citrinin-and-dihydroergocristine-compared-to-uv-irradiation-and-fenton-like-reaction
#7
Julia Keller, Hajo Haase, Matthias Koch
Mycotoxins occur widely in foodstuffs and cause a variety of mold-related health risks to humans and animals. Elucidation of the metabolic fate of mycotoxins and the growing number of newly discovered mycotoxins have enhanced the demand for fast and reliable simulation methods. The viability of electrochemistry coupled with mass spectrometry (EC/ESI-MS), Fenton-like oxidation, and UV irradiation for the simulation of oxidative phase I metabolism of the mycotoxins citrinin (CIT) and dihydroergocristine (DHEC) was investigated...
April 19, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28424857/extraction-of-cell-free-dna-from-urine-using-polylysine-coated-silica-particles
#8
Sho Takano, Qingjiang Hu, Takaki Amamoto, Paulo Refinetti, Koshi Mimori, Takashi Funatsu, Masaru Kato
DNA analysis is used for a variety of purposes, including disease diagnosis and DNA profiling; this involves extracting DNA from living organisms. In this study, we prepared polycationic silica particles to extract DNA that has the negatively charged phosphate backbone from solution. The coated particles were prepared by mixing conventional silica gel particles and poly-Lys; these particles could efficiently extract 1.3 μg of cell-free DNA from 50 mL of (male) urine. It is expected that these easily prepared particles (just a mixture of two commercially available chemicals) can be used as a noninvasive diagnostic tool for genetic disorders such as cancer, diabetes, and hypertension...
April 19, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28424856/homogenous-assay-for-protein-detection-based-on-proximity-dna-hybridization-and-isothermal-circular-strand-displacement-amplification-reaction
#9
Manjun Zhang, Ruimin Li, Liansheng Ling
This work proposed a homogenous fluorescence assay for proteins, based on the target-triggered proximity DNA hybridization in combination with strand displacement amplification (SDA). It benefited from target-triggered proximity DNA hybridization to specifically recognize the target and SDA making recycling signal amplification. The system included a molecular beacon (MB), an extended probe (EP), and an assistant probe (AP), which were not self-assembly in the absence of target proteins, due to the short length of the designed complementary sequence among MB, EP, and AP...
April 19, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28424855/analysis-of-multiple-soybean-phytonutrients-by-near-infrared-reflectance-spectroscopy
#10
Gaoyang Zhang, Penghui Li, Wenfei Zhang, Jian Zhao
Improvement of the nutritional quality of soybean is usually facilitated by a vast range of soybean germplasm with enough information about their multiple phytonutrients. In order to acquire this essential information from a huge number of soybean samples, a rapid analytic method is urgently required. Here, a nondestructive near-infrared reflectance spectroscopy (NIRS) method was developed for rapid and accurate measurement of 25 nutritional components in soybean simultaneously, including fatty acids palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid, vitamin E (VE), α-VE, γ-VE, δ-VE, saponins, isoflavonoids, and flavonoids...
April 19, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28417180/multiplexed-assay-for-protein-quantitation-in-the-invertebrate-gammarus-fossarum-by-liquid-chromatography-coupled-to-tandem-mass-spectrometry
#11
Aurore Charnot, Duarte Gouveia, Jean Armengaud, Christine Almunia, Arnaud Chaumot, Jérôme Lemoine, Olivier Geffard, Arnaud Salvador
A highly multiplexed liquid chromatography mass spectrometry-selected reaction monitoring (SRM)-based assay for determination of 40 potential protein biomarkers from Gammarus fossarum, an ecotoxicological relevant species, was described. The assay relies on 71 stable isotope-labeled reported peptide standards for the quantitation of proteins of interest in relation to essential physiological functions such as reproductive cycle, defense mechanism, and enzymes involved in homeostasis process and in energy. A direct linear relationship between the spiked peptide concentration and the area under the peak was clearly demonstrated in biological extracts...
April 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28417179/analysis-of-honeybush-tea-cyclopia-spp-volatiles-by-comprehensive-two-dimensional-gas-chromatography-using-a-single-stage-thermal-modulator
#12
Gaalebalwe Ntlhokwe, Andreas G J Tredoux, Tadeusz Górecki, Matthew Edwards, Jochen Vestner, Magdalena Muller, Lené Erasmus, Elizabeth Joubert, J Christel Cronje, André de Villiers
The applicability of comprehensive two-dimensional gas chromatography (GC×GC) using a single-stage thermal modulator was explored for the analysis of honeybush tea (Cyclopia spp.) volatile compounds. Headspace solid phase micro-extraction (HS-SPME) was used in combination with GC×GC separation on a non-polar × polar column set with flame ionisation (FID) detection for the analysis of fermented Cyclopia maculata, Cyclopia subternata and Cyclopia genistoides tea infusions of a single harvest season. Method optimisation entailed evaluation of the effects of several experimental parameters on the performance of the modulator, the choice of columns in both dimensions, as well as the HS-SPME extraction fibre...
April 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28417178/highly-sensitive-and-selective-determination-of-hydrogen-sulfide-by-resonance-light-scattering-technique-based-on-silver-nanoparticles
#13
Yangfang Kuang, Shu Chen, Yunfei Long
We have developed a green approach to prepare DNA-templated silver nanoparticles (Ag-NPs) from the direct reaction between Ag(+) and ascorbic acid in the presence of DNA and sodium hydroxide. The Ag-NPs showed strong resonance light scattering (RLS) intensity property. Then, the interaction between hydrogen sulfide (H2S) and Ag-NPs was studied by measuring their RLS spectra. The results showed that there is a strong interaction between Ag-NPs and H2S, which resulted in a decrease in the size of Ag-NPs and a decrease in the RLS intensity of the Ag-NPs solution at the wavelength of 467 nm...
April 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28417177/in-situ-hydrothermal-synthesis-of-titanium-dioxide-nanorods-on-titanium-wire-for-solid-phase-microextraction-of-polycyclic-aromatic-hydrocarbons
#14
Yu Tian, Juanjuan Feng, Yanan Bu, Xiuqin Wang, Chuannan Luo, Min Sun
Titanium dioxide nanorods were prepared on the surface of titanium wire by hydrothermal synthesis for use as a solid-phase microextraction (SPME) fiber. The morphology of the SPME coating was observed by scanning electron microscopy (SEM). Employed in conjunction with gas chromatography (GC), the fiber was investigated with five polycyclic aromatic hydrocarbons (PAHs) and three terphenyls in direct-immersion extraction mode. Various parameters were optimized, such as the extraction time, the stirring rate, the extraction temperature, the ionic strength of the sample solution, and the desorption time...
April 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28417176/functional-gold-nanoparticles-for-optical-affinity-biosensing
#15
Tomáš Špringer, Xue Chadtová Song, Maria Laura Ermini, Josefína Lamačová, Jiří Homola
Functional gold nanoparticles (AuNPs) are commonly used to enhance the response of optical affinity biosensors. In this work, we investigated the effect of preparation conditions on functional properties of AuNPs functionalized with antibody (Ab-AuNPs), specifically AuNPs with antibody against carcinoembryonic antigen (CEA) covalently attached via carboxy-terminated oligo-ethylene thiolate linker layer. The following parameters of preparation of Ab-AuNP have been found to have a significant effect on Ab-AuNP performance in affinity biosensors: the time of reaction of activated AuNPs with antibody, concentrations of antibody and amino-coupling reagents, and composition of immobilization buffer (molarity and salt content)...
April 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28417175/microwave-assisted-deglycosylation-for-rapid-and-sensitive-analysis-of-n-glycans-via-glycosylamine-derivatization
#16
Yike Wu, Chang Wang, Jing Luo, Yanyan Liu, Liang Zhang, Yun Xia, Xiaojun Feng, Bi-Feng Liu, Yawei Lin, Xin Liu
A rapid and sensitive N-glycan profiling strategy for MALDI-MS incorporating the use of deglycosylation with microwave assistance and the co-derivatization of glycosylamine labeling with tris(2,4,6-trimethoxyphenyl)phosphonium acetic acid N-hydroxysuccinimide ester (TMPP-Ac-OSu) and methylamidation has been developed in this work. Notably, highly efficient release and tagging of N-glycans from ribonuclease B was achieved in less than 90 min, providing up to 35-fold enhancement of MALDI-MS sensitivity with comparison to underivatized N-glycans...
April 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28417174/multidimensional-single-cell-analysis-based-on-fluorescence-microscopy-and-automated-image-analysis
#17
Michael Sandmann, Martin Lippold, Franziska Saalfrank, Chimezie Progress Odika, Sascha Rohn
A single-cell analytical technology was developed for evaluating fast-growing cultures of green algae. The main part of the single-cell analysis is an epifluorescence microscopy-based cytometric approach combined with an automated image analysis algorithm and a single-threshold discrimination procedure. The reliability of the technique in terms of object recognition, evaluating particle size, and determining chlorophyll was successfully proven via reference analyses. The microscopy technique was used to determine the size of single cells, the amount of chlorophyll, and the density of chlorophyll in a model algal culture (Acutodesmus o...
April 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28417173/semiautomated-taqman-pcr-screening-of-gmo-labelled-samples-for-unauthorised-gmos
#18
Ingrid M J Scholtens, Bonnie Molenaar, Richard A van Hoof, Stephanie Zaaijer, Theo W Prins, Esther J Kok
In most countries, systems are in place to analyse food products for the potential presence of genetically modified organisms (GMOs), to enforce labelling requirements and to screen for the potential presence of unauthorised GMOs. With the growing number of GMOs on the world market, a larger diversity of methods is required for informative analyses. In this paper, the specificity of an extended screening set consisting of 32 screening methods to identify different crop species (endogenous genes) and GMO elements was verified against 59 different GMO reference materials...
April 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28417172/simultaneous-determination-of-multiclass-pesticide-residues-in-human-plasma-using-a-mini-quechers-method
#19
Anshuman Srivastava, Satyajeet Rai, Ashish Kumar Sonker, Kajal Karsauliya, Chandra Prabha Pandey, Sheelendra Pratap Singh
Blood is one of the most assessable matrices for the determination of pesticide residue exposure in humans. Effective sample preparation/cleanup of biological samples is very important in the development of a sensitive, reproducible, and robust method. In the present study, a simple, cost-effective, and rapid gas chromatography-tandem mass spectrometry method has been developed and validated for simultaneous analysis of 31 multiclass (organophosphates, organochlorines, and synthetic pyrethroids) pesticide residues in human plasma by means of a mini QuEChERS (quick, easy, cheap, effective, rugged, and safe) method...
April 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28417171/resolution-optimized-headspace-gas-chromatography-ion-mobility-spectrometry-hs-gc-ims-for-non-targeted-olive-oil-profiling
#20
Natalie Gerhardt, Markus Birkenmeier, Daniel Sanders, Sascha Rohn, Philipp Weller
A prototype gas chromatography-ion mobility spectrometry (GC-IMS) system, hyphenating temperature-ramped headspace GC to a modified drift time IMS cell, was evaluated and compared to a conventional, isothermal capillary column (CC)-IMS system on the example of the geographical differentiation of extra virgin olive oils (EVOO) from Spain and Italy. It allows orthogonal, 2D separation of complex samples and individual detection of compounds in robust and compact benchtop systems. The information from the high-resolution 3D fingerprints of volatile organic compound (VOC) fractions of EVOO samples were extracted by specifically developed chemometric MATLAB® routines to differentiate between the different olive oil provenances...
April 17, 2017: Analytical and Bioanalytical Chemistry
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