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Analytical and Bioanalytical Chemistry

Lara F Stadlmair, Sylvia Grosse, Thomas Letzel, Jörg E Drewes, Johanna Grassmann
In this study, transformation products (TPs) of diclofenac, mefenamic acid, and sotalol derived from peroxidase- and laccase-catalyzed transformations were studied with different mass spectrometry (MS)-based workflows. A straightforward pre-screening of enzymatic degradation rate was performed using a robotic nano-ESI source coupled to single quadrupole MS. Accurate mass data and information on molecular hydrophobicity were obtained from a serial coupling of reversed phase liquid chromatography (RPLC) with hydrophilic interaction liquid chromatography (HILIC) to a time-of-flight-mass spectrometer (ToF-MS)...
November 12, 2018: Analytical and Bioanalytical Chemistry
Remington X Poulin, Georg Pohnert
Chemical signals are important mediators of organismal interactions. These interactions significantly influence ecosystem structure and thus are crucial to understand. Ecologists and analytical chemists work closely together to identify the specific molecules regulating ecological interactions. However, limitations in the analytical techniques on the one hand and time-demanding bioassays on the other have been restraining chemical ecology research. Application of metabolomics techniques has recently led to significant advancement of the field...
November 12, 2018: Analytical and Bioanalytical Chemistry
Ignacy Rzagalinski, Nadine Hainz, Carola Meier, Thomas Tschernig, Dietrich A Volmer
Multiple sclerosis (MS) is an immune-mediated neurodegenerative disease of the central nervous system (CNS). One of the most promising recent medications for MS is teriflunomide. Its primary mechanism of action is linked to effects on the peripheral immune system by inhibiting dihydroorotate dehydrogenase (DHODH)-catalyzed de novo pyrimidine synthesis and reducing the expansion of lymphocytes in the peripheral immune system. Some in vitro studies suggested, however, that it can also have a direct effect on the CNS compartment...
November 12, 2018: Analytical and Bioanalytical Chemistry
Rocio Costo, David Heinke, Cordula Grüttner, Fritz Westphal, M Puerto Morales, S Veintemillas-Verdaguer, Nicole Gehrke
Most iron oxide nanoparticles applications, and in special biomedical applications, require the accurate determination of iron content as the determination of particle properties from measurements in dispersions is strongly dependent on it. Inductively coupled plasma (ICP) and spectrophotometry are two typical worldwide used analytical methods for iron concentration determination. In both techniques, precise determination of iron is not straightforward and nanoparticle digestion and dilution procedures are needed prior to analysis...
November 12, 2018: Analytical and Bioanalytical Chemistry
Wei Wang, Yingyan Wang, Liyuan Lin, Yanling Song, Chaoyong James Yang
The traditional Gram-staining method, which was invented more than a century ago for differentiating bacteria as Gram positive or Gram negative, is still widely practiced in microbiology. However, Gram staining suffers from several problems which can affect the accuracy of the diagnosis. Here, we report a new Gram-negative-specific fluorescent probe, which is based on a narrow-spectrum antibiotic, tridecaptin A1, and allows selective staining of Gram-negative bacteria in different fixed bacterial samples. Solid-phase peptide synthesis was used to prepare the tridecaptin A1-fluorophore conjugate with a single structure...
November 10, 2018: Analytical and Bioanalytical Chemistry
Jürgen W Einax
No abstract text is available yet for this article.
November 10, 2018: Analytical and Bioanalytical Chemistry
Fujian Huang, Yan Chen, Yongqian Wang, Fan Xia
A superamphiphobic surface composed of two different size ranges of TiO2 nanoparticles was simply fabricated through spraying the perfluorosilane coated TiO2 nanoparticles suspension dispersing in ethanol. The surface chemistry was finely regulated through gradient UV irradiation-induced organic compound degradation to fabricate surface with gradient solid surface energy or wettability. The fabricated surface shows good droplet sorting ability, which can successfully discriminate ethanol droplets with different concentrations...
November 10, 2018: Analytical and Bioanalytical Chemistry
Alejandro Mahía, María Conde-Giménez, Sandra Salillas, Irantzu Pallarés, Juan J Galano-Frutos, Íñigo Lasa, Salvador Ventura, María D Díaz-de-Villegas, José A Gálvez, Javier Sancho
Amyloid fibrils formed by a variety of peptides are biological markers of different human diseases, such as Alzheimer's disease, Parkinson's disease, and type II diabetes, and are structural constituents of bacterial biofilms. Novel fluorescent probes offering improved sensitivity or specificity toward that diversity of amyloid fibrils or providing alternative spectral windows are needed to improve the detection or the identification of amyloid structures. One potential source for such new probes is offered by molecules known to interact with fibrils, such as the inhibitors of amyloid aggregation found in drug discovery projects...
November 9, 2018: Analytical and Bioanalytical Chemistry
Guan-Yuan Chen, Qibin Zhang
Oxylipins, a subclass of lipid mediators, are metabolites of various polyunsaturated fatty acids with crucial functions in regulation of systemic inflammation. Elucidation of their roles in pathological conditions requires accurate quantification of their levels in biological samples. We refined an ultra-performance liquid chromatography-multiple reaction monitoring-mass spectrometry (UPLC-MRM-MS)-based workflow for comprehensive and specific quantification of 131 endogenous oxylipins in human plasma, in which we optimized LC mobile phase additives, column, and gradient conditions...
November 8, 2018: Analytical and Bioanalytical Chemistry
Yanlong Lyu, Zhanhui Tao, Xiaodong Lin, Pengcheng Qian, Yunfei Li, Shuo Wang, Yaqing Liu
Ascorbic acid (AA) detection in biological sample and food sample is critical for human health. Herein, a MnO2 nanosheet (MnO2 -NS)-based ratiometric fluorescent nanosensor has been developed for high sensitive and specific detection of AA. The MnO2 -NS presents peroxidase-like activity and can oxidize non-fluorescent substrate of o-phenylenediamine (OPDA) into fluorescent substrate, presenting maximum fluorescence at 568 nm (F568 ). If MnO2 -NS is premixed with AA, the MnO2 -NS is then decomposed as Mn2+ by AA, decreasing the fluorescent intensity of F568 ...
November 7, 2018: Analytical and Bioanalytical Chemistry
Sofia Soares, Teresa Castro, Tiago Rosado, Nicolás Fernández, Mário Barroso, Eugenia Gallardo
This work describes the optimization of a new method for the determination of five organophosphorus insecticides in whole blood. The analytes were extracted from the matrix (50 μL) using the dried blood spot (DBS) approach and were analyzed by gas chromatography (GC) coupled to tandem mass spectrometry (MS/MS). The studied compounds (diazinon, chlorpyrifos, parathion-ethyl, chlorfenvinphos and quinalphos) were chosen based on the statistics of intoxications in Portugal, and ethion was used as internal standard...
November 7, 2018: Analytical and Bioanalytical Chemistry
Andrea M Giovannozzi, Andrea Hornemann, Beatrix Pollakowski-Herrmann, Felicia M Green, Paul Gunning, Tara L Salter, Rory T Steven, Josephine Bunch, Chiara Portesi, Bonnie J Tyler, Burkhard Beckhoff, Andrea Mario Rossi
Biomedical devices are complex products requiring numerous assembly steps along the industrial process chain, which can carry the potential of surface contamination. Cleanliness has to be analytically assessed with respect to ensuring safety and efficacy. Although several analytical techniques are routinely employed for such evaluation, a reliable analysis chain that guarantees metrological traceability and quantification capability is desirable. This calls for analytical tools that are cascaded in a sensible way to immediately identify and localize possible contamination, both qualitatively and quantitatively...
November 6, 2018: Analytical and Bioanalytical Chemistry
Laura Anfossi, Fabio Di Nardo, Alida Russo, Simone Cavalera, Cristina Giovannoli, Giulia Spano, Sabine Baumgartner, Kathrin Lauter, Claudio Baggiani
In this study, we report the simultaneous use of gold and silver nanoparticles to set a multicolor multiplex lateral flow immunoassay (xLFIA). Silver nanoparticles (AgNPs), spherical in shape and characterized by a brilliant yellow color, were obtained by a new viable one-step synthetic protocol. AgNPs were stable over time and acceptably robust to conditions used for fabricating LFIA devices. These AgNPs were employed as a colorimetric probe in combination with two different kinds of gold nanoparticles (AuNPs) to set a visual xLFIA for detecting allergens...
November 6, 2018: Analytical and Bioanalytical Chemistry
Philippe Corbisier, Hendrik Emons
The GM content in a food or feed product produced from or containing genetically modified organisms (GMO) has to be expressed in Europe in the form of a GM mass fraction. However, the most widely used quantification methods, based on PCR, are basically counting PCR-amplifiable DNA fragments in a sample extract. This paper outlines the requirements for obtaining comparable measurement results which are fit for regulatory decision-making. It introduces the concept of a reference measurement system which enables GMO analysis laboratories to relate their results to a universally accessible reference, thus establishing metrological traceability to a unique reference point...
November 6, 2018: Analytical and Bioanalytical Chemistry
Bradley B Stocks, Jeremy E Melanson
Disulfide bonds are critical linkages for maintaining protein structure and enzyme activity. These linkages, however, can limit peptide sequencing efforts by mass spectrometry (MS) and often require chemical reduction and alkylation. Under such conditions, information regarding cysteine connectivity is lost. Online partial disulfide reduction within the electrospray (ESI) source has recently been established as a means to identify complex cysteine linkage patterns in a liquid chromatography-MS experiment without the need for sample pre-treatment...
November 6, 2018: Analytical and Bioanalytical Chemistry
Maowei Dou, Christopher D Chouinard, Ying Zhu, Gabe Nagy, Andrey V Liyu, Yehia M Ibrahim, Richard D Smith, Ryan T Kelly
Mass spectrometry (MS)-based analysis of complex biological samples is essential for biomedical research and clinical diagnostics. The separation prior to MS plays a key role in the overall analysis, with separations having larger peak capacities often leading to more identified species and improved confidence in those identifications. High-resolution ion mobility (IM) separations enabled by Structures for Lossless Ion Manipulation (SLIM) can provide extremely rapid, high-resolution separations and are well suited as a second dimension of separation following nanoscale liquid chromatography (nanoLC)...
November 5, 2018: Analytical and Bioanalytical Chemistry
Tim Nagel, Florentine Klaus, Ines Gil Ibanez, Henning Wege, Ansgar Lohse, Bernd Meyer
Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC). Glycan structures from glycoproteins are usually altered in cancer. Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells. Glycosylation and phosphorylation of the protein species were quantified by liquid chromatography coupled to mass spectrometry to correlate PTMs to pathological cases...
November 5, 2018: Analytical and Bioanalytical Chemistry
Catarina Coutinho, Álvaro Somoza
MicroRNAs (miRNAs) are small regulatory RNAs, the dysregulation of which has been associated with the progression of several human diseases, including cancer. Interestingly, these molecules can be used as biomarkers for early disease diagnosis and can be found in a variety of body fluids and tissue samples. However, their specific properties and very low concentrations make their detection rather challenging. In this regard, current detection methods are complex, cost-ineffective, and of limited application in point-of-care settings or resource-limited facilities...
November 3, 2018: Analytical and Bioanalytical Chemistry
Qiaofeng Li, Jialei Bai, Shuyue Ren, Jiang Wang, Yifei Gao, Shuang Li, Yuan Peng, Baoan Ning, Zhixian Gao
Bisphenol A (BPA) is one of the endocrine-disrupting chemicals which might cause reproductive and endocrine system diseases, and poses a serious threat to the ecosystem and human health. This paper reports an ultrasensitive sensor for trace BPA detection employing fluorescence resonance energy transfer (FRET) between modified upconversion nanoparticles (UCNPs) and tetramethylrhodamine. To circumvent the problems of low luminous efficiency of FRET and low sensitivity of sensor, the upconversion nanoparticles with very strong fluorescence efficiency were prepared and quantitatively modified...
November 3, 2018: Analytical and Bioanalytical Chemistry
Ellen K Parker, Anna V Nielsen, Michael J Beauchamp, Haifa M Almughamsi, Jacob B Nielsen, Mukul Sonker, Hua Gong, Gregory P Nordin, Adam T Woolley
Preterm birth (PTB) is defined as birth before the 37th week of pregnancy and results in 15 million early deliveries worldwide every year. Presently, there is no clinical test to determine PTB risk; however, a panel of nine biomarkers found in maternal blood serum has predictive power for a subsequent PTB. A significant step in creating a clinical diagnostic for PTB is designing an automated method to extract and purify these biomarkers from blood serum. Here, microfluidic devices with 45 μm × 50 μm cross-section channels were 3D printed with a built-in polymerization window to allow a glycidyl methacrylate monolith to be site-specifically polymerized within the channel...
October 31, 2018: Analytical and Bioanalytical Chemistry
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