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Lab on a Chip

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https://www.readbyqxmd.com/read/28207921/pneumatic-stimulation-of-c-elegans-mechanoreceptor-neurons-in-a-microfluidic-trap
#1
Adam L Nekimken, Holger Fehlauer, Anna A Kim, Sandra N Manosalvas-Kjono, Purim Ladpli, Farah Memon, Divya Gopisetty, Veronica Sanchez, Miriam B Goodman, Beth L Pruitt, Michael Krieg
New tools for applying force to animals, tissues, and cells are critically needed in order to advance the field of mechanobiology, as few existing tools enable simultaneous imaging of tissue and cell deformation as well as cellular activity in live animals. Here, we introduce a novel microfluidic device that enables high-resolution optical imaging of cellular deformations and activity while applying precise mechanical stimuli to the surface of the worm's cuticle with a pneumatic pressure reservoir. To evaluate device performance, we compared analytical and numerical simulations conducted during the design process to empirical measurements made with fabricated devices...
February 16, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28207920/paper-based-microfluidic-system-for-tear-electrolyte-analysis
#2
Ali K Yetisen, Nan Jiang, Ali Tamayol, Guillermo U Ruiz-Esparza, Yu Shrike Zhang, Sofía Medina-Pando, Aditi Gupta, James S Wolffsohn, Haider Butt, Ali Khademhosseini, Seok-Hyun Yun
The analysis of tear constituents at point-of-care settings has a potential for early diagnosis of ocular disorders such as dry eye disease, low-cost screening, and surveillance of at-risk subjects. However, current minimally-invasive rapid tear analysis systems for point-of-care settings have been limited to assessment of osmolarity or inflammatory markers and cannot differentiate between dry eye subclassifications. Here, we demonstrate a portable microfluidic system that allows quantitative analysis of electrolytes in the tear fluid that is suited for point-of-care settings...
February 16, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28205656/an-autonomous-lab-on-a-chip-for-space-flight-calibration-of-gravity-induced-transcellular-calcium-polarization-in-single-cell-fern-spores
#3
J Park, M L Salmi, W W A Wan Salim, A Rademacher, B Wickizer, A Schooley, J Benton, A Cantero, P F Argote, M Ren, M Zhang, D M Porterfield, A J Ricco, S J Roux, J L Rickus
This report describes the development of lab-on-a-chip device designed to measure changes in cellular ion gradients that are induced by changes in gravitational (g) forces. The bioCD presented here detects differential calcium ion concentrations outside of individual cells. The device includes sufficient replicates for statistical analysis of the gradients around multiple single cells and around control wells that are empty or include dead cells. In the data presented, the degree of the cellular response correlates with the magnitude of the g-force applied via rotation of the bioCD...
February 16, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28205652/bridging-the-gap-microfluidic-devices-for-short-and-long-distance-cell-cell-communication
#4
REVIEW
Timothy Quang Vu, Ricardo Miguel Bessa de Castro, Lidong Qin
Cell-cell communication is a crucial component of many biological functions. For example, understanding how immune cells and cancer cells interact, both at the immunological synapse and through cytokine secretion, can help us understand and improve cancer immunotherapy. The study of how cells communicate and form synaptic connections is important in neuroscience, ophthalmology, and cancer research. But in order to increase our understanding of these cellular phenomena, better tools need to be developed that allow us to study cell-cell communication in a highly controlled manner...
February 16, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28205650/photobleaching-kinetics-based-bead-encoding-for-multiplexed-bioassays
#5
Thomas H Linz, W Hampton Henley, J Michael Ramsey
Multiplexing bead-based bioassays requires that each type of microsphere be uniquely encoded to distinguish one type from another. Microspheres are typically encoded using fluorescent dyes with different spectral properties and varying concentrations. However practical limits exist on the number of dyes that can be spectrally resolved or the number of distinguishable intensity levels with each dye. To expand the number of encoding levels, a novel method was developed that incorporates photobleaching kinetics into bead decoding, unlocking additional multiplexing levels unattainable by conventional decoding methods...
February 16, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28198493/correction-the-magnitude-of-lift-forces-acting-on-drops-and-bubbles-in-liquids-flowing-inside-microchannels
#6
Claudiu A Stan, Audrey K Ellerbee, Laura Guglielmini, Howard A Stone, George M Whitesides
Correction for 'The magnitude of lift forces acting on drops and bubbles in liquids flowing inside microchannels' by Claudiu A. Stan et al., Lab Chip, 2013, 13, 365-376.
February 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28197611/anisotropic-sliding-on-dual-rail-hydrophilic-tracks
#7
Xiaolong Yang, Jinlong Song, Huanxi Zheng, Xu Deng, Xin Liu, Xiaohong Lu, Jing Sun, Danyang Zhao
Biomimetic surfaces with sliding angle (SA) anisotropy have the capacity to directionally control the motion of water droplets and therefore have wide applications in various domains. Parallel and narrowing dual-rail hydrophilic tracks (DRHTs) are fabricated on etched superhydrophobic Al surfaces using a micromilling technique. Orthogonal and linear SA anisotropies are observed and investigated on the parallel and narrowing DRHTs, respectively. Track spacings of the parallel DRHTs are designed to regulate the orthogonal SA anisotropy of the water droplet...
February 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28197602/automating-multi-step-paper-based-assays-using-integrated-layering-of-reagents
#8
Sana Jahanshahi-Anbuhi, Balamurali Kannan, Kevin Pennings, M Monsur Ali, Vincent Leung, Karen Giang, Jingyun Wang, Dawn White, Yingfu Li, Robert H Pelton, John D Brennan, Carlos D M Filipe
We describe a versatile and simple method to perform sequential reactions on paper analytical devices by stacking dry pullulan films on paper, where each film contains one or more reagents or acts as a delay layer. Exposing the films to an aqueous solution of the analyte leads to sequential dissolution of the films in a temporally controlled manner followed by diffusive mixing of the reagents, so that sequential reactions can be performed. The films can be easily arranged for lateral flow assays or for spot tests (reactions take place sequentially in the z-direction)...
February 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28197601/active-droplet-sorting-in-microfluidics-a-review
#9
REVIEW
Heng-Dong Xi, Hao Zheng, Wei Guo, Alfonso M Gañán-Calvo, Ye Ai, Chia-Wen Tsao, Jun Zhou, Weihua Li, Yanyi Huang, Nam-Trung Nguyen, Say Hwa Tan
The ability to manipulate and sort droplets is a fundamental issue in droplet-based microfluidics. Various lab-on-a-chip applications can only be realized if droplets are systematically categorized and sorted. These micron-sized droplets act as ideal reactors which compartmentalize different biological and chemical reagents. Array processing of these droplets hinges on the competence of the sorting and integration into the fluidic system. Recent technological advances only allow droplets to be actively sorted at the rate of kilohertz or less...
February 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28197593/tandem-emulsification-for-high-throughput-production-of-double-emulsions
#10
M L Eggersdorfer, W Zheng, S Nawar, C Mercandetti, A Ofner, I Leibacher, S Koehler, D A Weitz
Core-shell double emulsions produced using microfluidic methods with controlled structural parameters exhibit great potential in a wide range of applications, but the low production rate of microfluidic methods hinders the exploitation of the capabilities of microfluidics to produce double emulsions with well-defined features. A major obstacle towards the scaled-up production of core-shell double emulsions is the difficulty of achieving robust spatially controlled wettability in integrated microfluidic devices...
February 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28197586/determination-of-the-temperature-dependent-cell-membrane-permeabilities-using-microfluidics-with-integrated-flow-and-temperature-control
#11
Cifeng Fang, Fujun Ji, Zhiquan Shu, Dayong Gao
We developed an integrated microfluidic platform for instantaneous flow and localized temperature control. The platform consisted of a flow-focusing region for sample delivery and a cross-junction region embedded with a microheater for cell trapping and localized temperature control by using an active feedback control system. We further used it to measure the membrane transport properties of Jurkat cells, including the osmotically inactive cell volume (Vb) and cell membrane permeabilities to water (Lp) and to cryoprotective agent (CPA) solutions (dimethyl sulfoxide (DMSO) in this study) (PS) at various temperatures (room temperature, 30 °C, and 37 °C)...
February 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28197582/wearable-microfluidics-fabric-based-digital-droplet-flowmetry-for-perspiration-analysis
#12
Yahui Yang, Siyuan Xing, Zecong Fang, Ruya Li, Helen Koo, Tingrui Pan
The latest development in wearable technologies has attracted much attention. In particular, collection and analysis of body fluids has been a focus. In this paper, we have reported a wearable microfluidic platform made using conventional fabric materials and laser micromachining to measure the flow rate on a patterned fabric surface, referred to as digital droplet flowmetry (DDF). The proposed wearable DDF is capable of collecting and measuring continuous perspiration with high precision (96% on average) in a real-time fashion over a defined area of skin...
February 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28194463/improved-bovine-embryo-production-in-an-oviduct-on-a-chip-system-prevention-of-poly-spermic-fertilization-and-parthenogenic-activation
#13
Marcia A M M Ferraz, Heiko H W Henning, Pedro F Costa, Jos Malda, Ferry P Melchels, R Wubbolts, Tom A E Stout, Peter L A M Vos, Bart M Gadella
The oviduct provides the natural micro-environment for gamete interaction, fertilization and early embryo development in mammals, such as the cow. In conventional culture systems, bovine oviduct epithelial cells (BOEC) undergo a rapid loss of essential differentiated cell properties; we aimed to develop a more physiological in vitro oviduct culture system capable of supporting fertilization. U-shaped chambers were produced using stereo-lithography and mounted with polycarbonate membranes, which were used as culture inserts for primary BOECs...
February 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28194461/a-3d-microblade-structure-for-precise-and-parallel-droplet-splitting-on-digital-microfluidic-chips
#14
Cheng Dong, Yanwei Jia, Jie Gao, Tianlan Chen, Pui-In Mak, Mang-I Vai, Rui P Martins
Existing digital microfluidic (DMF) chips exploit the electrowetting on dielectric (EWOD) force to perform droplet splitting. However, the current splitting methods are not flexible and the volume of the droplets suffers from a large variation. Herein, we propose a DMF chip featuring a 3D microblade structure to enhance the droplet-splitting performance. By exploiting the EWOD force for shaping and manipulating the mother droplet, we obtain an average dividing error of <2% in the volume of the daughter droplets for a number of fluids such as deionized water, DNA solutions and DNA-protein mixtures...
February 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28194457/disposable-cartridge-platform-for-rapid-detection-of-viral-hemorrhagic-fever-viruses
#15
Steven M Scherr, David S Freedman, Krystle N Agans, Alexandru Rosca, Erik Carter, Melody Kuroda, Helen E Fawcett, Chad E Mire, Thomas W Geisbert, M Selim Ünlü, John H Connor
Light microscopy is a straightforward and highly portable imaging approach that is used for the detection of parasites, fungi, and bacteria. The detection of individual virus particles has historically not been possible through this approach. Thus, characterization of virus particles is typically performed using high-energy approaches such as electron microscopy. These approaches require purification of virions away from its normal milieu, significant levels of expertise, and only count a small number of particles at a time...
February 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28184395/microfluidic-platform-for-rapid-measurement-of-transepithelial-water-transport
#16
Byung-Ju Jin, A S Verkman
Water transport across epithelial monolayers is of central importance in mammalian fluid homeostasis, and epithelial aquaporin (AQP) water channels are potential drug targets. Current methods to measure transepithelial water permeability based on indicator dilution have limited accuracy and can require hours for a single measurement. We report here a microfluidics platform for rapid and accurate measurement of water transport across a conventionally cultured epithelial monolayer on a porous filter requiring only a single image obtained using a standard laboratory fluorescence microscope...
February 10, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28184386/cell-agglomeration-in-the-wells-of-a-24-well-plate-using-acoustic-streaming
#17
Yuta Kurashina, Kenjiro Takemura, James Friend
Cell agglomeration is essential both to the success of drug testing and to the development of tissue engineering. Here, a MHz-order acoustic wave is used to generate acoustic streaming in the wells of a 24-well plate to drive particle and cell agglomeration. Acoustic streaming is known to manipulate particles in microfluidic devices, and even provide concentration in sessile droplets, but concentration of particles or cells in individual wells has never been shown, principally due to the drag present along the periphery of the fluid in such a well...
February 10, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28181607/robust-temperature-change-rate-actuated-valving-and-switching-for-highly-integrated-centrifugal-microfluidics
#18
M Keller, G Czilwik, J Schott, I Schwarz, K Dormanns, F von Stetten, R Zengerle, N Paust
We present new unit operations for valving and switching in centrifugal microfluidics that are actuated by a temperature change rate (TCR) and controlled by the rotational frequency. Implementation is realized simply by introducing a comparatively large fluidic resistance to an air vent of a fluidic structure downstream of a siphon channel. During temperature decrease at a given TCR, the air pressure inside the downstream structure decreases and the fluidic resistance of the air vent slows down air pressure compensation allowing a thermally induced underpressure to build up temporarily...
February 9, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28165521/high-selectivity-cytology-via-lab-on-a-disc-western-blotting-of-individual-cells
#19
John J Kim, Elly Sinkala, Amy E Herr
Cytology of sparingly available cell samples from both clinical and experimental settings would benefit from high-selectivity protein tools. To minimize cell handling losses in sparse samples, we design a multi-stage assay using a lab-on-a-disc that integrates cell handling and subsequent single-cell western blotting (scWestern). As the two-layer microfluidic device rotates, the induced centrifugal force directs dissociated cells to dams, which in turn localize the cells over microwells. Cells then sediment into the microwells, where the cells are lysed and subjected to scWestern...
February 6, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28164203/modulating-motility-of-intracellular-vesicles-in-cortical-neurons-with-nanomagnetic-forces-on-chip
#20
Anja Kunze, Coleman Tylor Murray, Chanya Godzich, Jonathan Lin, Keegan Owsley, Andy Tay, Dino Di Carlo
Vesicle transport is a major underlying mechanism of cell communication. Inhibiting vesicle transport in brain cells results in blockage of neuronal signals, even in intact neuronal networks. Modulating intracellular vesicle transport can have a huge impact on the development of new neurotherapeutic concepts, but only if we can specifically interfere with intracellular transport patterns. Here, we propose to modulate motion of intracellular lipid vesicles in rat cortical neurons based on exogenously bioconjugated and cell internalized superparamagnetic iron oxide nanoparticles (SPIONs) within microengineered magnetic gradients on-chip...
February 6, 2017: Lab on a Chip
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