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Lab on a Chip

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https://www.readbyqxmd.com/read/28740980/micro-engineered-perfusable-3d-vasculatures-for-cardiovascular-diseases
#1
Nishanth Venugopal Menon, Hui Min Tay, Soon Nan Wee, King Ho Holden Li, Han Wei Hou
Vessel geometries in microengineered in vitro vascular models are important to recapitulate a pathophysiological microenvironment for the study of flow-induced endothelial dysfunction and inflammation in cardiovascular diseases. Herein, we present a simple and novel extracellular matrix (ECM) hydrogel patterning method to create perfusable vascularized microchannels of different geometries based on the concept of capillary burst valve (CBV). No surface modification is necessary and the method is suitable for different ECM types including collagen, matrigel and fibrin...
July 25, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28736788/characterisation-of-anticancer-peptides-at-the-single-cell-level
#2
L Armbrecht, G Gabernet, F Kurth, J A Hiss, G Schneider, P S Dittrich
The development of efficacious anticancer therapeutics is difficult due to the heterogeneity of the cellular response to chemotherapy. Anticancer peptides (ACPs) are promising drug candidates that have been shown to be active against a range of cancer cells. However, few ACP studies focus on tumour single-cell heterogeneities. In order to address this need, we developed a microfluidic device and an imaging procedure that enable the capture, monitoring, and analysis of several hundred single cells for the study of drug response...
July 24, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28731104/a-new-droplet-forming-fluidic-junction-for-the-generation-of-highly-compartmentalised-capsules
#3
J Li, D A Barrow
A new oscillatory microfluidic junction is described, which enables the consistent formation of highly uniform and complex double emulsions, and is demonstrated for the encapsulation of four different reagents within the inner droplets (called cores) of the double emulsion droplets. Once the double emulsion droplets had attained a spherical form, the cores assumed specific 3D arrangements, the orchestration of which appeared to depend upon the specific emulsion morphology. Such double emulsion droplets were used as templates to produce highly compartmentalised microcapsules and multisomes...
July 21, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28731086/microfluidic-and-nanofluidic-phase-behaviour-characterization-for-industrial-co2-oil-and-gas
#4
REVIEW
Bo Bao, Jason Riordon, Farshid Mostowfi, David Sinton
Microfluidic systems that leverage unique micro-scale phenomena have been developed to provide rapid, accurate and robust analysis, predominantly for biomedical applications. These attributes, in addition to the ability to access high temperatures and pressures, have motivated recent expanded applications in phase measurements relevant to industrial CO2, oil and gas applications. We here present a comprehensive review of this exciting new field, separating microfluidic and nanofluidic approaches. Microfluidics is practical, and provides similar phase properties analysis to established bulk methods with advantages in speed, control and sample size...
July 21, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28726927/custom-3d-printer-and-resin-for-18-%C3%AE-m-%C3%A3-20-%C3%AE-m-microfluidic-flow-channels
#5
Hua Gong, Bryce P Bickham, Adam T Woolley, Gregory P Nordin
While there is great interest in 3D printing for microfluidic device fabrication, to-date the achieved feature sizes have not been in the truly microfluidic regime (<100 μm). In this paper we demonstrate that a custom digital light processor stereolithographic (DLP-SLA) 3D printer and a specifically-designed, low cost, custom resin can readily achieve flow channel cross sections as small as 18 μm × 20 μm. Our 3D printer has a projected image plane resolution of 7.6 μm and uses a 385 nm LED, which dramatically increases the available selection of UV absorbers for resin formulation compared to 3D printers with 405 nm LEDs...
July 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28726923/robust-manufacturing-of-lipid-polymer-nanoparticles-through-feedback-control-of-parallelized-swirling-microvortices
#6
Michael J Toth, Taeyoung Kim, YongTae Kim
A variety of therapeutic and/or diagnostic nanoparticles (NPs), or nanomedicines, have been formulated for improved drug delivery and imaging applications. Microfluidic technology enables continuous and highly reproducible synthesis of NPs through controlled mixing processes at the micro- and nanoscale. Yet, the inherent low-throughput remains a critical roadblock, precluding the probable applications of new nanomedicines for clinical translation. Here we present robust manufacturing of lipid-polymer NPs (LPNPs) through feedback controlled operation of parallelized swirling microvortex reactors (SMRs)...
July 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28726916/a-novel-3-d-bio-microfluidic-system-mimicking-in-vivo-heterogeneous-tumour-microstructures-reveals-complex-tumour-stroma-interactions
#7
Qihui Fan, Ruchuan Liu, Yang Jiao, Chunxiu Tian, James D Farrell, Wenwen Diao, Xiaochen Wang, Fengrong Zhang, Wei Yuan, Haibo Han, Jinfeng Chen, Yue Yang, Xixiang Zhang, Fangfu Ye, Ming Li, Zhongcan Ouyang, Liyu Liu
A 3-D microfluidic system consisting of microchamber arrays embedded in a collagen hydrogel with tuneable biochemical gradients that mimics the tumour microenvironment of mammary glands was constructed for the investigation on the interactions between invasive breast cancer cells and stromal cells. The hollow microchambers in collagen provide a very similar 3-D environment to that in vivo that regulates collective cellular dynamics and behaviour, while the microfluidic channels surrounding the collagen microchamber arrays allow one to impose complex concentration gradients of specific biological molecules or drugs...
July 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28726914/3d-printed-smartphone-based-point-of-care-tool-for-fluorescence-and-magnetophoresis-based-cytometry
#8
Stephanie Knowlton, Ashwini Joshi, Philip Syrrist, Ahmet F Coskun, Savas Tasoglu
In developing countries, there are often limited resources available to provide important medical diagnostics, which severely limits our ability to diagnose conditions and administer proper treatment, leading to high mortality rates for treatable conditions. Here, we propose a multiplex tool capable of density-based cell sorting via magnetic focusing in parallel with fluorescence imaging to provide highly specific clinical assays. While many cell sorting techniques and fluorescence microscopes generally are costly and require extensive user training, limiting accessibility and usability in developing countries, this device is compact, low-cost, and portable...
July 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28726912/3d-material-cytometry-3dmac-a-very-high-replicate-high-throughput-analytical-method-using-microfabricated-shape-specific-cell-material-niches
#9
Kirsten Parratt, Jenny Jeong, Peng Qiu, Krishnendu Roy
Studying cell behavior within 3D material niches is key to understanding cell biology in health and diseases, and developing biomaterials for regenerative medicine applications. Current approaches to studying these cell-material niches have low throughput and can only analyze a few replicates per experiment resulting in reduced measurement assurance and analytical power. Here, we report 3D material cytometry (3DMaC), a novel high-throughput method based on microfabricated, shape-specific 3D cell-material niches and imaging cytometry...
July 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28725897/integration-and-application-of-optical-chemical-sensors-in-microbioreactors
#10
Pia Gruber, Marco P C Marques, Nicolas Szita, Torsten Mayr
The quantification of key variables such as oxygen, pH, carbon dioxide, glucose, and temperature provides essential information for biological and biotechnological applications and their development. Microfluidic devices offer an opportunity to accelerate research and development in these areas due to their small scale, and the fine control over the microenvironment, provided that these key variables can be measured. Optical sensors are well-suited for this task. They offer non-invasive and non-destructive monitoring of the mentioned variables, and the establishment of time-course profiles without the need for sampling from the microfluidic devices...
July 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28722051/holographic-microscope-slide-in-a-spatio-temporal-imaging-modality-for-reliable-3d-cell-counting
#11
Biagio Mandracchia, Vittorio Bianco, Zhe Wang, Martina Mugnano, Alessia Bramanti, Melania Paturzo, Pietro Ferraro
In the current trend of miniaturization and simplification of imaging flow cytometry, Lab-on-a-Chip (LoC) microfluidic devices represent an innovative and cost-effective solution. In this framework, we propose for the first time a novel platform based on the compactness of a holographic microscope slide (HMS) in combination with the new computational features of space-time digital holography (STDH) that uses a 1D linear sensor array (LSA) instead of 2D CCD or CMOS cameras to respond to real diagnostic needs...
July 19, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28718848/label-free-high-throughput-holographic-screening-and-enumeration-of-tumor-cells-in-blood
#12
Dhananjay Kumar Singh, Caroline C Ahrens, Wei Li, Siva A Vanapalli
We introduce inline digital holographic microscopy (in-line DHM) as a label-free technique for detecting tumor cells in blood. The optimized DHM platform fingerprints every cell flowing through a microchannel at 10 000 cells per second, based on three features - size, maximum intensity and mean intensity. To identify tumor cells in a background of blood cells, we developed robust gating criteria using machine-learning approaches. We established classifiers from the features extracted from 100 000-cell training sets consisting of red blood cells, peripheral blood mononuclear cells and tumor cell lines...
July 18, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28717801/mimicking-arterial-thrombosis-in-a-3d-printed-microfluidic-in-vitro-vascular-model-based-on-computed-tomography-angiography-data
#13
Pedro F Costa, Hugo J Albers, John E A Linssen, Heleen H T Middelkamp, Linda van der Hout, Robert Passier, Albert van den Berg, Jos Malda, Andries D van der Meer
Arterial thrombosis is the main instigating factor of heart attacks and strokes, which result in over 14 million deaths worldwide every year. The mechanism of thrombosis involves factors from the blood and the vessel wall, and it also relies strongly on 3D vessel geometry and local blood flow patterns. Microfluidic chip-based vascular models allow controlled in vitro studies of the interaction between vessel wall and blood in thrombosis, but until now, they could not fully recapitulate the 3D geometry and blood flow patterns of real-life healthy or diseased arteries...
July 18, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28714506/an-integrated-microwell-array-platform-for-cell-lasing-analysis
#14
Qiushu Chen, Yu-Cheng Chen, Zhizheng Zhang, Biming Wu, Rhima Coleman, Xudong Fan
Biological cell lasers are emerging as a novel technology in biological studies and biomedical engineering. The heterogeneity of cells, however, can result in various lasing behaviors from cell to cell. Thus, the capability to track individual cells during laser investigation is highly desired. In this work, a microwell array was integrated with high-quality Fabry-Pérot cavities for addressable and automated cell laser studies. Cells were captured in the microwells and the corresponding cell lasing was achieved and analyzed using SYTO9-stained Sf9 cells as a model system...
July 17, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28702612/rapid-label-free-cd4-testing-using-a-smartphone-compatible-device
#15
Manoj Kumar Kanakasabapathy, Hardik J Pandya, Mohamed Shehata Draz, Manjyot Kaur Chug, Magesh Sadasivam, Shreya Kumar, Behzad Etemad, Vinish Yogesh, Mohammadali Safavieh, Waseem Asghar, Jonathan Z Li, Athe M Tsibris, Daniel R Kuritzkes, Hadi Shafiee
The most recent guidelines have called for a significant shift towards viral load testing for HIV/AIDS management in developing countries; however point-of-care (POC) CD4 testing still remains an important component of disease staging in multiple developing countries. Advancements in micro/nanotechnologies and consumer electronics have paved the way for mobile healthcare technologies and the development of POC smartphone-based diagnostic assays for disease detection and treatment monitoring. Here, we report a simple, rapid (30 minutes) smartphone-based microfluidic chip for automated CD4 testing using a small volume (30 μL) of whole blood...
July 12, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28702608/point-of-care-testing-applications-of-3d-printing
#16
REVIEW
Ho Nam Chan, Ming Jun Andrew Tan, Hongkai Wu
Point-of-care testing (POCT) devices fulfil a critical need in the modern healthcare ecosystem, enabling the decentralized delivery of imperative clinical strategies in both developed and developing worlds. To achieve diagnostic utility and clinical impact, POCT technologies are immensely dependent on effective translation from academic laboratories out to real-world deployment. However, the current research and development pipeline is highly bottlenecked owing to multiple restraints in material, cost, and complexity of conventionally available fabrication techniques...
July 12, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28686280/spectral-reading-of-optical-resonance-encoded-cells-in-microfluidics
#17
Matjaž Humar, Avinash Upadhya, Seok Hyun Yun
The ability to label individual cells is useful for single-cell-level studies of complex cellular interactions and heterogeneity. Optically readable cell labeling is attractive as it can be investigated non-invasively and repeatedly at high speeds. Here, we demonstrate the feasibility of large-scale cell barcoding and identification using fluorescent polystyrene microbeads loaded into cells. Intracellular beads with different diameters in a range of 5 to 12 μm generate spectrally distinguished features or barcodes...
July 7, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28685786/on-chip-cell-sorting-by-high-speed-local-flow-control-using-dual-membrane-pumps
#18
Shinya Sakuma, Yusuke Kasai, Takeshi Hayakawa, Fumihito Arai
Although researchers have proposed various methods of on-chip cell sorting, high-throughput sorting of large cells remains hampered by the difficulty of controlling high-speed flow over a wide sorting area. To overcome this problem, we proposed high-speed local-flow control using dual membrane pumps driven by piezoelectric actuators placed on the outside of a microfluidic chip in this paper. We evaluated the controllability of shifting the flow profile by the local-flow. The results indicated that we could sort large cells up to approximately 150 μm in size with an equivalent throughput of 31 kHz...
July 7, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28682395/an-automated-optofluidic-biosensor-platform-combining-interferometric-sensors-and-injection-moulded-microfluidics
#19
C Szydzik, A F Gavela, S Herranz, J Roccisano, M Knoerzer, P Thurgood, K Khoshmanesh, A Mitchell, L M Lechuga
A primary limitation preventing practical implementation of photonic biosensors within point-of-care platforms is their integration with fluidic automation subsystems. For most diagnostic applications, photonic biosensors require complex fluid handling protocols; this is especially prominent in the case of competitive immunoassays, commonly used for detection of low-concentration, low-molecular weight biomarkers. For this reason, complex automated microfluidic systems are needed to realise the full point-of-care potential of photonic biosensors...
July 6, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28671705/organ-body-and-disease-on-a-chip-systems
#20
EDITORIAL
Michael L Shuler
No abstract text is available yet for this article.
July 3, 2017: Lab on a Chip
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