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Lab on a Chip

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https://www.readbyqxmd.com/read/29327010/dna-methylation-assay-using-droplet-based-dna-melting-curve-analysis
#1
F-W Liu, H-F Liao, S-P Lin, Y-W Lu
DNA methylation is an epigenetic regulation of gene expression, which has drawn great attention in biomedical research due to its association with various diseases. A robust, inexpensive platform to detect and quantify the methylation status in a specific genomic region is necessary. In this study, an on-chip analytical technique of cytosine methylation with droplets in a microchannel is proposed. Genomic DNA samples are encapsulated into a series of droplets and transported through a detection region, where a stable temperature gradient is created...
January 12, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29326990/a-fluorescent-microbead-based-microfluidic-immunoassay-chip-for-immune-cell-cytokine-secretion-quantification
#2
Xin Cui, Ya Liu, Dinglong Hu, Weiyi Qian, Chung Tin, Dong Sun, Weiqiang Chen, Raymond H W Lam
Quantitative and dynamic analyses of immune cell secretory cytokines are essential for precise determination and characterization of the "immune phenotype" of patients for clinical diagnosis and treatment of immune-related diseases. Although multiple methods including the enzyme-linked immunosorbent assay (ELISA) have been applied for cytokine detection, such measurements remain very challenging in real-time, high-throughput, and high-sensitivity immune cell analysis. In this paper, we report a highly integrated microfluidic device that allows for on-chip isolation, culture, and stimulation, as well as sensitive and dynamic cytokine profiling of immune cells...
January 12, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29318250/razor-printed-sticker-microdevices-for-cell-based-applications
#3
Loren E Stallcop, Yasmín R Álvarez-García, Ana M Reyes-Ramos, Karla P Ramos-Cruz, Molly M Morgan, Yatao Shi, Lingjun Li, David J Beebe, Maribella Domenech, Jay W Warrick
Tape-based razor-printing is a flexible and affordable ultra-rapid prototyping approach for microscale device fabrication. However, integration of this prototyping approach into cell-based assay development has been limited to proof of principle demonstrations. This is in large part due to lack of an established or well-characterized option for biocompatible adhesive tape. Without such an option, integration of these areas will remain unexplored. Therefore, to address this critical hurdle, we characterized microscale devices made using a potentially biocompatible double-sided adhesive, ARCare 90106...
January 10, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29313859/correction-towards-microwave-imaging-of-cells
#4
Mehmet Kelleci, Hande Aydogmus, Levent Aslanbas, Selcuk Oguz Erbil, M Selim Hanay
Correction for 'Towards microwave imaging of cells' by Mehmet Kelleci et al., Lab Chip, 2018, DOI: 10.1039/c7lc01251a.
January 9, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29313542/a-programmable-platform-for-sub-second-multichemical-dynamic-stimulation-and-neuronal-functional-imaging-in-c-elegans
#5
T Rouse, G Aubry, Y Cho, M Zimmer, H Lu
Caenorhabditis elegans (C. elegans) is a prominent model organism in neuroscience, as its small stereotyped nervous system offers unique advantages for studying neuronal circuits at the cellular level. Characterizing temporal dynamics of neuronal circuits is essential to fully understand neuronal processing. Characterization of the temporal dynamics of chemosensory circuits requires a precise and fast method to deliver multiple stimuli and monitor the animal's neuronal activity. Microfluidic platforms have been developed that offer an improved control of chemical delivery compared to manual methods...
January 9, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29309079/fundamentals-of-rapid-injection-molding-for-microfluidic-cell-based-assays
#6
Ulri N Lee, Xiaojing Su, David J Guckenberger, Ashley M Dostie, Tianzi Zhang, Erwin Berthier, Ashleigh B Theberge
Microscale cell-based assays have demonstrated unique capabilities in reproducing important cellular behaviors for diagnostics and basic biological research. As these assays move beyond the prototyping stage and into biological and clinical research environments, there is a need to produce microscale culture platforms more rapidly, cost-effectively, and reproducibly. 'Rapid' injection molding is poised to meet this need as it enables some of the benefits of traditional high volume injection molding at a fraction of the cost...
January 8, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29309077/nanofiber-membrane-supported-lung-on-a-chip-microdevice-for-anti-cancer-drug-testing
#7
Xingyuan Yang, Kaiyan Li, Xu Zhang, Chang Liu, Bingkun Guo, Weijia Wen, Xinghua Gao
Organ-on-a-chip technology can simulate the physiological and pathological microenvironment of tissues and organs in vitro, thus offering the potential of dispensing with animal models to predict the toxicity and efficacy of therapies. In this study, taking the alveolar microenvironment as a model, we developed a lung-on-a-chip with a poly(lactic-co-glycolic acid) (PLGA) electrospinning nanofiber membrane as the chip substrate and cell scaffold. The PLGA nanofiber membrane, with a controlled thickness of ∼3 μm, is porous and permeable to molecules, has good biocompatibility, and offers a means to simulate the alveolar respiratory membrane...
January 8, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29303179/capillary-based-integrated-digital-pcr-in-picoliter-droplets
#8
Jinyu Chen, Zhaofeng Luo, Lin Li, Jinlong He, Luoquan Li, Jianwei Zhu, Ping Wu, Liqun He
The droplet digital polymerase chain reaction (ddPCR) is becoming more and more popular in diagnostic applications in academia and industry. In commercially available ddPCR systems, after they have been made by a generator, the droplets have to be transferred manually to modules for amplification and detection. In practice, some of the droplets (∼10%) are lost during manual transfer, leading to underestimation of the targets. In addition, the droplets are also at risk of cross-contamination during transfer...
January 5, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29302660/acoustofluidic-devices-controlled-by-cell-phones
#9
Hunter Bachman, Po-Hsun Huang, Shuaiguo Zhao, Shujie Yang, Peiran Zhang, Hai Fu, Tony Jun Huang
Acoustofluidic devices have continuously demonstrated their potential to impact medical diagnostics and lab-on-a-chip applications. To bring these technologies to real-world applications, they must be made more accessible to end users. Herein, we report on the effort to provide an easy-to-use and portable system for controlling sharp-edge-based acoustofluidic devices. With the use of a cell phone and a modified Bluetooth® speaker, on-demand and hands-free pumping and mixing are achieved. Additionally, a novel design for a sharp-edge-based acoustofluidic device is proposed that combines both pumping and mixing functions into a single device, thus removing the need for external equipment typically needed to accomplish these two tasks...
January 5, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29300407/acoustically-modulated-biomechanical-stimulation-for-human-cartilage-tissue-engineering
#10
Umesh S Jonnalagadda, Martyn Hill, Walid Messaoudi, Richard B Cook, Richard O C Oreffo, Peter Glynne-Jones, Rahul S Tare
Bioacoustofluidics can be used to trap and levitate cells within a fluid channel, thereby facilitating scaffold-free tissue engineering in a 3D environment. In the present study, we have designed and characterised an acoustofluidic bioreactor platform, which applies acoustic forces to mechanically stimulate aggregates of human articular chondrocytes in long-term levitated culture. By varying the acoustic parameters (amplitude, frequency sweep, and sweep repetition rate), cells were stimulated by oscillatory fluid shear stresses, which were dynamically modulated at different sweep repetition rates (1-50 Hz)...
January 4, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29299579/buffer-free-integrative-nanofluidic-device-for-real-time-continuous-flow-bioassays-by-ion-concentration-polarization
#11
Dinh-Tuan Phan, Lin Jin, Shofarul Wustoni, Chia-Hung Chen
To perform precision medicine in real-time, a sensor capable of continuously monitoring target biomolecules secreted from a patient under dynamic situations is essential. In this study, a novel portable device combining an aptamer probe and a nanofluidic component was developed, enabling the buffer-free continuous monitoring of small molecules in biological fluids. This integration is synergistic: the aptamer sensor is used to bind target biomolecules, triggering a fluorescence signal change, while the nanofluidic component is applied to achieve ion concentration polarization and convert serum into a clean buffer for aptamer signal regeneration...
January 4, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29299576/linking-invasive-motility-to-protein-expression-in-single-tumor-cells
#12
Jung-Ming G Lin, Chi-Chih Kang, Yun Zhou, Haiyan Huang, Amy E Herr, Sanjay Kumar
The invasion of malignant cells into tissue is a critical step in the progression of cancer. While it is increasingly appreciated that cells within a tumor differ in their invasive potential, it remains nearly unknown how these differences relate to cell-to-cell variations in protein expression. Here, we introduce a microfluidic platform that integrates measurements of invasive motility and protein expression for single cells, which we use to scrutinize human glioblastoma tumor-initiating cells (TICs). Our live-cell imaging microdevice is comprised of polyacrylamide microchannels that exhibit tissue-like stiffness and present chemokine gradients along each channel...
January 4, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29297916/engineering-reaction-diffusion-networks-with-properties-of-neural-tissue
#13
Thomas Litschel, Michael M Norton, Vardges Tserunyan, Seth Fraden
We present an experimental system of networks of coupled non-linear chemical reactors, which we theoretically model within a reaction-diffusion framework. The networks consist of patterned arrays of diffusively coupled nanoliter-scale reactors containing the Belousov-Zhabotinsky (BZ) reaction. Microfluidic fabrication techniques are developed that provide the ability to vary the network topology and the reactor coupling strength and offer the freedom to choose whether an arbitrary reactor is inhibitory or excitatory coupled to its neighbor...
January 3, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29297912/temperature-change-rate-actuated-bubble-mixing-for-homogeneous-rehydration-of-dry-pre-stored-reagents-in-centrifugal-microfluidics
#14
S Hin, N Paust, M Keller, M Rombach, O Strohmeier, R Zengerle, K Mitsakakis
In centrifugal microfluidics, dead volumes in valves downstream of mixing chambers can hardly be avoided. These dead volumes are excluded from mixing processes and hence cause a concentration gradient. Here we present a new bubble mixing concept which avoids such dead volumes. The mixing concept employs heating to create a temperature change rate (TCR) induced overpressure in the air volume downstream of mixing chambers. The main feature is an air vent with a high fluidic resistance, representing a low pass filter with respect to pressure changes...
January 3, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29264613/dynamic-wettability-of-polyethylene-glycol-modified-poly-dimethylsiloxane-surfaces-in-an-aqueous-organic-two-phase-system
#15
Mao Fukuyama, Manabu Tokeshi, Mikhail A Proskurnin, Akihide Hibara
We herein report the preparation of a surface that behaves in a hydrophobic manner but does not undergo protein adsorption in an aqueous/organic two-phase system. We found that polyethylene-glycol (PEG)-modified poly(dimethylsiloxane) (PDMS) exhibits hydrophobic properties when the surface is immersed in an organic solution, while the PEG moiety prevents protein adsorption on the PDMS surface in an aqueous solution at high protein concentrations due to the dynamic behaviour of the PEG moiety. As such, we demonstrated the in-well droplet formation of an aqueous solution containing a high protein concentration...
December 21, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29264612/microfluidic-bypass-manometry-highly-parallelized-measurement-of-flow-resistance-of-complex-channel-geometries-and-trapped-droplets
#16
Naureen S Suteria, Mehdi Nekouei, Siva A Vanapalli
Current lithography methods allow facile fabrication of microfluidic conduits where not only the shape of the bounding walls can be arbitrarily varied but also the internal conduit space can be laden with a variety of microstructures and wetting properties. This virtually infinite design space of microfluidic geometries brings in the challenge of how to quantify fluid resistance in a large number of microfluidic conduits, while maintaining operational simplicity. We report a versatile experimental technique referred to as microfluidic bypass manometry for measurement of pressure drop versus flow rate (ΔP-Q) relations in a parallelized manner...
December 21, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29260185/electrochemical-biosensor-for-cancer-cell-detection-based-on-a-surface-3d-micro-array
#17
Li An, Guangtong Wang, Yu Han, Tianchan Li, Peng Jin, Shaoqin Liu
The detection of rare circulating tumour cells (CTCs) in patients' blood is crucial for the early diagnosis of cancer, highly precise cancer therapy and monitoring therapeutic outcomes in real time. In this study we have developed an efficient strategy to capture and detect CTCs from the blood of cancer patients using a benzoboric acid modified gold-plated polymeric substrate with a regular 3D surface array. Compared with the smooth substrate, the substrate with the surface 3D microarrays exhibited a higher capture efficiency, i...
December 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29244051/towards-microwave-imaging-of-cells
#18
Mehmet Kelleci, Hande Aydogmus, Levent Aslanbas, Selcuk Oguz Erbil, M Selim Hanay
Integrated detection techniques that can characterize the morphological properties of cells are needed for the widespread use of lab-on-a-chip technology. Herein, we establish a theoretical and experimental framework to use resonant microwave sensors in their higher order modes so that the morphological properties of analytes inside a microfluidic channel can be obtained electronically. We built a phase-locked loop system that can track the first two modes of a microstrip line resonator to detect the size and location of microdroplets and cells passing through embedded microfluidic channels...
December 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29242870/high-throughput-double-emulsion-based-microfluidic-production-of-hydrogel-microspheres-with-tunable-chemical-functionalities-toward-biomolecular-conjugation
#19
Eric Y Liu, Sukwon Jung, David A Weitz, Hyunmin Yi, Chang-Hyung Choi
Chemically functional hydrogel microspheres hold significant potential in a range of applications including biosensing, drug delivery, and tissue engineering due to their high degree of flexibility in imparting a range of functions. In this work, we present a simple, efficient, and high-throughput capillary microfluidic approach for controlled fabrication of monodisperse and chemically functional hydrogel microspheres via formation of double emulsion drops with an ultra-thin oil shell as a sacrificial template...
December 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29236123/converting-colour-to-length-based-on-the-coffee-ring-effect-for-quantitative-immunoassays-using-a-ruler-as-readout
#20
Dagan Zhang, Bingbing Gao, Yangtian Chen, Hong Liu
We report a method for converting the colorimetric results of an enzyme-linked immunosorbent assay (ELISA) into length based on the coffee-ring effect, so that the quantitative detection of analytes can be carried out simply using a ruler. The influence of the shape and lamination of the paper strip on the test results is studied. As a demonstration, human IgG is quantitatively analyzed. It is found that the width of the colored stains correlates with the concentration of the analyte which can be measured for quantitative analysis...
December 13, 2017: Lab on a Chip
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