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Lab on a Chip

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https://www.readbyqxmd.com/read/27910972/a-liver-microphysiological-system-of-tumor-cell-dormancy-and-inflammatory-responsiveness-is-affected-by-scaffold-properties
#1
A M Clark, S E Wheeler, C L Young, L Stockdale, J Shepard Neiman, W Zhao, D B Stolz, R Venkataramanan, D Lauffenburger, L Griffith, A Wells
Distant metastasis is the major cause of breast cancer-related mortality, commonly emerging clinically after 5 or more years of seeming 'cure' of the primary tumor, indicating a quiescent dormancy. The lack of relevant accessible model systems for metastasis that recreate this latent stage has hindered our understanding of the molecular basis and the development of therapies against these lethal outgrowths. We previously reported on the development of an all-human 3D ex vivo hepatic microphysiological system that reproduces several features of liver physiology and enables spontaneous dormancy in a subpopulation of breast cancer cells...
December 2, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27909706/out-of-plane-integration-of-a-multimode-optical-fiber-for-single-particle-cell-detection-at-multiple-points-on-a-microfluidic-device-with-applications-to-particle-cell-counting-velocimetry-size-discrimination-and-the-analysis-of-single-cell-lysate-injections
#2
Jalal Sadeghi, Damith E W Patabadige, Anne H Culbertson, Hamid Latifi, Christopher T Culbertson
In this paper a single particle/cell-tracking microfluidic device that integrates an out-of-plane multimode optical fiber (OP-MMF) is reported. This OP-MMF is used to generate three excitation light-lines and three detection spots using only one excitation source and one detector. It takes advantage of an optical tunneling mode to create two excitation lines in a microfluidic channel emanating from a single fiber end. This method was used to accurately count particles/cells and perform velocity measurements and size discrimination...
December 2, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27901159/a-portable-and-reconfigurable-multi-organ-platform-for-drug-development-with-onboard-microfluidic-flow-control
#3
J R Coppeta, M J Mescher, B C Isenberg, A J Spencer, E S Kim, A R Lever, T J Mulhern, R Prantil-Baun, J C Comolli, J T Borenstein
The drug development pipeline is severely limited by a lack of reliable tools for prediction of human clinical safety and efficacy profiles for compounds at the pre-clinical stage. Here we present the design and implementation of a platform technology comprising multiple human cell-based tissue models in a portable and reconfigurable format that supports individual organ function and crosstalk for periods of up to several weeks. Organ perfusion and crosstalk are enabled by a precision flow control technology based on electromagnetic actuators embedded in an arrayed format on a microfluidic platform...
November 30, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27896351/on-chip-integration-of-organic-synthesis-and-hplc-ms-analysis-for-monitoring-stereoselective-transformations-at-the-micro-scale
#4
Josef J Heiland, Rico Warias, Carsten Lotter, Laura Mauritz, Patrick J W Fuchs, Stefan Ohla, Kirsten Zeitler, Detlev Belder
We present a microfluidic system, seamlessly integrating microflow and microbatch synthesis with a HPLC/nano-ESI-MS functionality on a single glass chip. The microfluidic approach allows to efficiently steer and dispense sample streams down to the nanoliter-range for studying reactions in quasi real-time. In a proof-of-concept study, the system was applied to explore amino-catalyzed reactions, including asymmetric iminium-catalyzed Friedel-Crafts alkylations in microflow and micro confined reaction vessels.
November 29, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27891537/on-demand-magnetic-manipulation-of-liquid-metal-in-microfluidic-channels-for-electrical-switching-applications
#5
Jinpyo Jeon, Jeong-Bong Lee, Sang Kug Chung, Daeyoung Kim
We report magnetic-field-driven on-demand manipulation of liquid metal in microfluidic channels filled with base or acid. The liquid metal was coated with iron (Fe) particles and treated with hydrochloric acid to have strong bonding strength with the Fe particles. The magnetic liquid metal slug inserted in the microchannel is manipulated, merged, and separated. In addition, corresponding to the repositioning of an external magnet, the liquid metal slug can be readily moved in microfluidic channels with different angles (>90°) and cross-linked channels in any direction...
November 28, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27883138/characterization-of-size-dependent-mechanical-properties-of-tip-growing-cells-using-a-lab-on-chip-device
#6
Chengzhi Hu, Gautam Munglani, Hannes Vogler, Tohnyui Ndinyanka Fabrice, Naveen Shamsudhin, Falk K Wittel, Christoph Ringli, Ueli Grossniklaus, Hans J Herrmann, Bradley J Nelson
Quantification of mechanical properties of tissues, living cells, and cellular components is crucial for the modeling of plant developmental processes such as mechanotransduction. Pollen tubes are tip-growing cells that provide an ideal system to study the mechanical properties at the single cell level. In this article, a lab-on-a-chip (LOC) device is developed to quantitatively measure the biomechanical properties of lily (Lilium longiflorum) pollen tubes. A single pollen tube is fixed inside the microfluidic chip at a specific orientation and subjected to compression by a soft membrane...
November 24, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27883136/continuous-micro-vortex-based-nanoparticle-manipulation-via-focused-surface-acoustic-waves
#7
David J Collins, Zhichao Ma, Jongyoon Han, Ye Ai
Despite increasing demand in the manipulation of nanoscale objects for next generation biological and industrial processes, there is a lack of methods for reliable separation, concentration and purification of nanoscale objects. Acoustic methods have proven their utility in contactless manipulation of microscale objects mainly relying on the acoustic radiation effect, though the influence of acoustic streaming has typically prevented manipulation at smaller length scales. In this work, however, we explicitly take advantage of the strong acoustic streaming in the vicinity of a highly focused, high frequency surface acoustic wave (SAW) beam emanating from a series of focused 6 μm substrate wavelength interdigital transducers patterned on a piezoelectric lithium niobate substrate and actuated with a 633 MHz sinusoidal signal...
November 24, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27883132/microfluidic-pdms-on-paper-pop-devices
#8
Jin-Wen Shangguan, Yu Liu, Jian-Bin Pan, Bi-Yi Xu, Jing-Juan Xu, Hong-Yuan Chen
In this paper, we propose a generalized concept of microfluidic polydimethylsiloxane (PDMS) on paper (POP) devices, which combines well the merits of paper chips and PDMS chips. First, we optimized the conditions for accurate PDMS spatial patterning on paper, based on screen printing and a high temperature enabled superfast curing technique, which enables PDMS patterning to an accuracy of tens of microns in less than ten seconds. This, in turn, makes it available for seamless, reversible and reliable integration of the resulting paper layer with other PDMS channel structures...
November 24, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27869911/controlled-production-of-sub-millimeter-liquid-core-hydrogel-capsules-for-parallelized-3d-cell-culture
#9
Hugo Doméjean, Mathieu de la Motte Saint Pierre, Anette Funfak, Nicolas Atrux-Tallau, Kevin Alessandri, Pierre Nassoy, Jérôme Bibette, Nicolas Bremond
Liquid core capsules having a hydrogel membrane are becoming a versatile tool for three-dimensional culture of micro-organisms and mammalian cells. Making sub-millimeter capsules at a high rate, via the breakup of a compound jet in air, opens the way to high-throughput screening applications. However, control of the capsule size monodispersity, especially required for quantitative bioassays, was still lacking. Here, we report how the understanding of the underlying hydrodynamic instabilities that occur during the process can lead to calibrated core-shell bioreactors...
November 21, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27868127/digital-dna-detection-based-on-a-compact-optofluidic-laser-with-ultra-low-sample-consumption
#10
Wonsuk Lee, Qiushu Chen, Xudong Fan, Dong Ki Yoon
DNA lasers self-amplify optical signals from a DNA analyte as well as thermodynamic differences between sequences, allowing quasi-digital DNA detection. However, these systems have drawbacks, such as relatively large sample consumption and complicated dye labelling. Moreover, although the lasing signal can detect the target DNA, it is superimposed on an unintended fluorescence background, which persists for non-target DNA samples as well. From an optical point of view, it is thus not truly digital detection and requires spectral analysis to identify the target...
November 21, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27858042/dual-patterned-immunofiltration-dif-device-for-the-rapid-efficient-negative-selection-of-heterogeneous-circulating-tumor-cells
#11
Jiyoon Bu, Yoon-Tae Kang, Young Jun Kim, Young-Ho Cho, Hee Jin Chang, Hojoong Kim, Byung-In Moon, Ho Gak Kim
The analysis of circulating tumor cells (CTCs) is an emerging field for estimating the metastatic relapse and tumor burden of cancer patients. However, the isolation of CTCs is still challenging due to their ambiguity, rarity, and heterogeneity. Here, we present an anti-CD45 antibody based dual-patterned immunofiltration (DIF) device for the enrichment of heterogeneous CTC subtypes by effective elimination of leukocytes. Our uniquely designed dual-patterned layers significantly enhance the binding chance between immuno-patterns and leukocytes due to the fluidic whirling and the increased binding sites, thus achieving superior negative selection in terms of high-throughput and high purity...
November 18, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27858026/a-portable-microfluidic-system-for-rapid-measurement-of-the-erythrocyte-sedimentation-rate
#12
Ziya Isiksacan, Ozcan Erel, Caglar Elbuken
The erythrocyte sedimentation rate (ESR) is a frequently used 30 min or 60 min clinical test for screening of several inflammatory conditions, infections, trauma, and malignant diseases, as well as non-inflammatory conditions including prostate cancer and stroke. Erythrocyte aggregation (EA) is a physiological process where erythrocytes form face-to-face linear structures, called rouleaux, at stasis or low shear rates. In this work, we proposed a method for ESR measurement from EA. We developed a microfluidic opto-electro-mechanical system, using which we experimentally showed a significant correlation (R(2) = 0...
November 18, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27847939/on-chip-electromagnetic-tweezers-3-dimensional-particle-actuation-using-microwire-crossbar-arrays
#13
Philipp Rinklin, Hans-Joachim Krause, Bernhard Wolfrum
Emerging miniaturization technologies for biological and bioengineering applications require precise control over position and actuation of microparticles. While many of these applications call for high-throughput approaches, common tools for particle manipulation, such as magnetic or optical tweezers, suffer from low parallelizability. To address this issue, we introduce a chip-based platform that enables flexible three-dimensional control over individual magnetic microparticles. Our system relies on microwire crossbar arrays for simultaneous generation of magnetic and dielectric forces, which actuate the particles along highly localized traps...
November 16, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27841886/passive-and-active-droplet-generation-with-microfluidics-a-review
#14
REVIEW
Pingan Zhu, Liqiu Wang
Precise and effective control of droplet generation is critical for applications of droplet microfluidics ranging from materials synthesis to lab-on-a-chip systems. Methods for droplet generation can be either passive or active, where the former generates droplets without external actuation, and the latter makes use of additional energy input in promoting interfacial instabilities for droplet generation. A unified physical understanding of both passive and active droplet generation is beneficial for effectively developing new techniques meeting various demands arising from applications...
November 14, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27841430/single-cell-isolation-by-a-modular-single-cell-pipette-for-rna-sequencing
#15
Kai Zhang, Min Gao, Zechen Chong, Ying Li, Xin Han, Rui Chen, Lidong Qin
Single-cell transcriptome sequencing highly requires a convenient and reliable method to rapidly isolate a live cell into a specific container such as a PCR tube. Here, we report a modular single-cell pipette (mSCP) consisting of three modular components, a SCP-Tip, an air-displacement pipette (ADP), and ADP-Tips, that can be easily assembled, disassembled, and reassembled. By assembling the SCP-Tip containing a hydrodynamic trap, the mSCP can isolate single cells from 5-10 cells per μL of cell suspension...
November 14, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27833945/microdroplet-chain-array-for-cell-migration-assays
#16
Yan Ma, Jian-Zhang Pan, Shi-Ping Zhao, Qi Lou, Ying Zhu, Qun Fang
Establishing cell migration assays in multiple different microenvironments is important in the study of tissue repair and regeneration, cancer progression, atherosclerosis, and arthritis. In this work, we developed a miniaturized and massive parallel microfluidic platform for multiple cell migration assays combining the traditional membrane-based cell migration technique and the droplet-based microfluidic technique. Nanoliter-scale droplets are flexibly assembled as building blocks based on a porous membrane to form microdroplet chains with diverse configurations for different assay modes...
November 11, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27831583/multiplexed-droplet-interface-bilayer-formation
#17
Nathan E Barlow, Guido Bolognesi, Anthony J Flemming, Nicholas J Brooks, Laura M C Barter, Oscar Ces
We present a simple method for the multiplexed formation of droplet interface bilayers (DIBs) using a mechanically operated linear acrylic chamber array. To demonstrate the functionality of the chip design, a lipid membrane permeability assay is performed. We show that multiple, symmetric DIBs can be created and separated using this robust low-cost approach.
November 10, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27830852/advancements-in-microfluidics-for-nanoparticle-separation
#18
REVIEW
Thoriq Salafi, Kerwin Kwek Zeming, Yong Zhang
Nanoparticles have been widely implemented for healthcare and nanoscience industrial applications. Thus, efficient and effective nanoparticle separation methods are essential for advancement in these fields. However, current technologies for separation, such as ultracentrifugation, electrophoresis, filtration, chromatography, and selective precipitation, are not continuous and require multiple preparation steps and a minimum sample volume. Microfluidics has offered a relatively simple, low-cost, and continuous particle separation approach, and has been well-established for micron-sized particle sorting...
November 10, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27830849/review-imaging-technologies-for-flow-cytometry
#19
REVIEW
Yuanyuan Han, Yi Gu, Alex Ce Zhang, Yu-Hwa Lo
High-throughput single cell imaging is a critical enabling and driving technology in molecular and cellular biology, biotechnology, medicine and related areas. Imaging flow cytometry combines the single-cell imaging capabilities of microscopy with the high-throughput capabilities of conventional flow cytometry. Recent advances in imaging flow cytometry are remarkably revolutionizing single-cell analysis. This article describes recent imaging flow cytometry technologies and their challenges.
November 10, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27824367/enantioselective-reaction-monitoring-utilizing-two-dimensional-heart-cut-liquid-chromatography-on-an-integrated-microfluidic-chip
#20
Carsten Lotter, Elisabeth Poehler, Josef J Heiland, Laura Mauritz, Detlev Belder
Chip-integrated, two-dimensional high performance liquid chromatography is introduced to monitor enantioselective continuous micro-flow synthesis. The herein described development of the first two-dimensional HPLC-chip was realized by the integration of two different columns packed with reversed-phase and chiral stationary phase material on a microfluidic glass chip, coupled to mass spectrometry. Directed steering of the micro-flows at the joining transfer cross enabled a heart-cut operation mode to transfer the chiral compound of interest from the first to the second chromatographic dimension...
November 8, 2016: Lab on a Chip
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