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Doklady. Biochemistry and Biophysics

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https://www.readbyqxmd.com/read/28726109/the-study-of-immunogenicity-of-the-antigenic-protein-of-high-risk-oncogenic-type-of-the-human-papillomavirus-hpv16-l1-produced-in-the-plant-expression-system-on-the-base-of-transgenic-tomato
#1
R K Salyaev, N I Rekoslavskaya, A S Stolbikov, A V Tretyakova
The immunogenicity of plant-made peroral vaccine against cervical cancer was studied in mice during 342 days after vaccination with the material of tomato fruits genetically transformed with HPV16 L1 Ώ 5'UTR TMV. The immune response was found on day 4 after vaccination in blood serum of vaccinated mice. On days 90-100, the rise to maximum value of the level of antibodies to HPV16 L1 was in the range of 500 ng of the standard antibodies to HPV16 L1 (Santa Cruz Biotechnology, United States). This level of antibodies was retained until the end of the study...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726108/why-does-the-bioluminescent-fungus-armillaria-mellea-have-luminous-mycelium-but-nonluminous-fruiting-body
#2
K V Purtov, V N Petushkov, N S Rodionova, J I Gitelson
By determining the components involved in the bioluminescence process in luminous and nonluminous organs of the honey fungus Armillaria mellea, we have established causes of partial luminescence of this fungus. The complete set of enzymes and substrates required for bioluminescence is formed only in the mycelium and only under the conditions of free oxygen access. Since the synthesis of luciferin precursor (hispidin) and 3-hydroxyhispidin hydroxylase in the fruiting bodies is blocked, the formation of luciferin-the key component of fungal bioluminescent system-was not observed...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726107/studying-a-novel-ecdysone-dependent-enhancer
#3
J V Nikolenko, A N Krasnov, M Y Mazina, S G Georgieva, N E Vorobyeva
Earlier [4], we developed a two-stage scheme of the activation of expression of the ecdysone cascade gene ftz-f1 in Drosophila melanogaster S2 cells. In the first intron of the ftz-f1 gene, we found a binding site for the transcription activator DHR3. In this work, we studied the properties of this genomic element: the change in the histone modification level at different stages of transcription activation were analyzed, and data indicating the interaction of the intron element with the promoter were obtained...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726106/arginine-derivatives-of-dicarboxylic-acids-from-the-parotid-gland-secretions-of-common-toad-bufo-bufo-new-agonists-of-ionotropic-%C3%AE-aminobutyric-acid-receptors
#4
D S Lebedev, I A Ivanov, E V Kryukova, V G Starkov, V I Tsetlin, Yu N Utkin
Compounds activating γ-aminobutyric acid type A receptor were isolated from the toad Bufo bufo venom as a result of chromatographic separation. Analysis of the structure of these compounds by mass spectrometry and nuclear magnetic resonance showed that they are arginine derivatives of dicarboxylic acids and represent suberylarginine, pimeloylarginine, and adipoylarginine.
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726105/changes-in-levels-of-metabolic-pathway-gene-expression-under-conditions-of-clear-cell-renal-carcinoma
#5
V Yu Bashmakov, T M Gorbacheva, A V Panevina, S A Solodskikh, I P Moshurov, A A Mikhaylov, A Yu Maslov, V N Popov
A whole-transcriptome analysis of gene expression in six samples of clear cell renal cancer was performed. Using bioinformatics methods, we established a relationship between gene expression data and changes in activity of metabolic pathways: in this cancer type, the expression of genes involved in the metabolism of carbohydrates, lipids, and amino acids as well as in energy metabolism changed most significantly.
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726104/n-acyl-dopamines-induce-apoptosis-in-pc12-cell-line-via-the-gpr55-receptor-activation
#6
M G Akimov, A M Ashba, N M Gretskaya, V V Bezuglov
Dopamine amides of arachidonic, docosahexaenoic, and oleic acids were found to induce apoptosis in PC12 cells, which was blocked exclusively by antagonists and preincubation agonists of the receptor GPR55, belonging to the group of non-CB1/CB2 receptors.
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726103/the-effect-of-chlorpromazine-on-intracellular-ca-2-concentration-in-macrophages
#7
Z I Krutetskaya, L S Milenina, A A Naumova, S N Butov, V G Antonov, A D Nozdrachev
Using Fura-2AM microfluorimetry, it was shown for the first time that neuroleptic chlorpromazine causes intracellular Ca(2+) concentration increase in macrophages due to Ca(2+) mobilization from intracellular Ca(2+) stores and subsequent Ca(2+) entry from the external medium. Chlorpromazine-induced Ca(2+) entry is inhibited by La(3+) and 2-aminoethoxydiphenyl borate and is associated with Ca(2+) store depletion.
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726102/stereoselective-biotransformation-of-phenylglycine-nitrile-by-heterogeneous-biocatalyst-based-on-immobilized-bacterial-cells-and-enzyme-preparation
#8
Yu G Maksimova, A N Gorbunova, V A Demakov
We studied the effect of a heterogeneous environment on the stereoselectivity of transformation of racemic phenylglycine nitrile. Immobilized biocatalysts were prepared by adhesion of Pseudomonas fluorescens C2 cells on carbon-containing supports and covalent crosslinking of nitrile hydratase and amidase of Rhodococcus rhodochrous 4-1 to activated chitosan as well as by the method of cross-linked aggregates. At a reaction duration of 20 h, the ratio of phenylglycine stereoisomers changes depending on the presence of support in medium...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726101/eukaryotic-elongation-factor-2-kinase-activation-in-m-soleus-under-14-day-hindlimb-unloading-of-rats
#9
Y N Lomonosova, S P Belova, T M Mirzoev, I B Kozlovskaya, B S Shenkman
Functional unloading of m. soleus of male Wistar rats was found to cause a reduction in protein synthesis. The level of phosphorylation of the translation elongation factor 2 (eEF2) and the eEF2 kinase (eEF2k) activity in m. soleus after 14 days of unloading were assessed. Rats were divided into the control group (C) and the group with hindlimb unloading for 14 days (HU14). The level of eEF2 phosphorylation in group HU14 was 80%, whereas in the control is was 40%. The indices of eEF2k expression and protein content in group HU14 increased compared to group C...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726100/microrna-in-hormonal-mechanisms-of-regulation-of-nk-cell-function
#10
S V Shirshev, I V Nekrasova, O L Gorbunova, E G Orlova, I L Maslennikova
We investigated the effect of human chorionic gonadotropin, estriol, leptin, ghrelin, and kisspeptin on the microRNA expression in separated NK cells. All of these hormones are able to effectively modulate the expression of microRNAs both stimulating and suppressing the cytotoxic potential of NK cells and thereby indirectly regulate the functions of these lymphocytes.
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726099/lipoxygenases-modulate-the-effect-of-glutoxim-on-na-transport-in-the-frog-skin-epithelium
#11
Z I Krutetskaya, A V Melnitskaya, V G Antonov, A D Nozdrachev
Using voltage-clamp technique, the involvement of lipoxygenases in the effect of immunomodulatory drug glutoxim on Na(+) transport in frog skin was investigated. It was shown for the first time that preincubation of the skin with lipoxygenase inhibitors caffeic acid, baicalein, and nordihydroguaiaretic acid significantly decreases the stimulatory effect of glutoxim on Na(+) transport. The data suggest the involvement of lipoxygenase oxidation pathway of arachidonic acid metabolism in the glutoxim effect on Na(+) transport in frog skin epithelium...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726098/magnetic-dependent-atp-pool-in-escherichia-coli
#12
U G Letuta, E I Avdeeva
The ATP pool in Escherichia coli is a magnetic-dependent characteristic of microorganism vital activity. It depends on the values of the external static magnetic field and the existence of magnetic moment of magnesium isotopes nuclei added to the growth nutrient medium. The combined effects of the magnetic field 70-95 mT and magnesium magnetic isotope (25)Mg on E. coli bacteria leads to increase intracellular concentration of ATP. Magnetic-field effects in the range of 0.8-16 mT, registered for all bacteria regardless of the magnesium-isotopic enrichment of nutrient medium, evidence about the sensitivity of intracellular processes to weak magnetic fields...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726097/kinetics-of-dna-elongation
#13
V I Bykov, A V Lukovenkov, S D Varfolomeev
A mathematical model of the DNA elongation stage is proposed, and a formula for calculating the characteristic time of doubling a DNA strand consisting of a large numbers of nucleotides was derived. It is shown that, when the number of nucleotides is greater than 50, it is fair to use a linear dependence of the elongation time on the number of nucleotides. Calculations of the DNA elongation time for a number of organisms are given.
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726096/identification-and-characterization-of-the-flower-meristem-identity-gene-mhylfy-in-mycoheterotrophic-plant-monotropa-hypopitys
#14
A V Shchennikova, O A Shulga, A V Beletsky, M A Filyushin, E Z Kochieva, N V Ravin, K G Skryabin
The gene encoding the transcription factor LEAFY was identified in the genome of the mycoheterotrophic plant, pinesap Monotropa hypopitys. In the transcriptomes of roots, bracts, and flowers of flowering pinesaps, the MhyLFY gene expression was absent. These data suggest the conservativeness of the LFY-dependent mechanism of flower meristem identity and flower formation in heterotrophic species with some differences associated to the specificity of development and the structure of such plants. The pinesap flowering under the control of the transcription factor MhyLFY may be initiated either in an embryonic inflorescence during spring dormancy release of adventitious root buds or in an inflorescence of a growing reproductive stem after photoperiodic induction...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726095/fatty-acid-composition-of-fish-species-with-different-feeding-habits-from-an-arctic-lake
#15
M I Gladyshev, N N Sushchik, L A Glushchenko, V A Zadelenov, A E Rudchenko, Y Y Dgebuadze
We compared the composition and content of fatty acids (FAs) in fish with different feeding habits (sardine (least) cisco Coregonus sardinella, goggle-eyed charr (pucheglazka) form of Salvelinus alpinus complex, humpback whitefish Coregonus pidschian, broad whitefish Coregonus nasus, boganid charr Salvelinus boganidae, and northern pike Esox lucius from an Arctic Lake. Feeding habits of the studied fish (planktivore, benthivore, or piscivore) significantly affected the composition of biomarker fatty acids and the ratio of stable isotopes of carbon and nitrogen in their biomass...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726094/gene-expression-and-content-of-enzymes-of-noradrenaline-synthesis-in-the-rat-organ-of-zuckerkandl-at-the-critical-period-of-morphogenesis
#16
A R Murtazina, Yu O Nikishina, N S Bondarenko, A Ya Sapronova, E V Volina, M V Ugrumov
Gene expression and content of the key enzymes involved in the synthesis of noradrenaline-tyrosine hydroxylase and dopamine beta-hydroxylase-was evaluated in the organ of Zuckerkandl of rats in the critical period of morphogenesis. High levels of mRNA and protein of both enzymes in the perinatal period of development and their sharp decline on day 30 of postnatal development were detected. These data indicate that the synthesis of noradrenaline in the organ of Zuckerkandl is maximum during the critical period of morphogenesis and decreases during the involution of this paraganglion...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726093/the-fluorescence-lifetime-of-lipofuscin-granule-fluorophores-contained-in-the-retinal-pigment-epithelium-cells-from-human-cadaver-eyes-in-normal-state-and-in-the-case-of-visualized-pathology
#17
M A Yakovleva, T B Feldman, P M Arbukhanova, S A Borzenok, V A Kuzmin, M A Ostrovsky
A comparative analysis of fluorescence lifetime of lipofuscin granule fluorophores contained in the retinal pigment epithelium cells from human cadaver eyes in normal state and in the case of visualized pathology was carried out. Measurements of fluorescence lifetimes of bis-retinoids and their photooxidation and photodegradation products were carried out using the method of counting time-correlated photons. Comparative analysis showed that, in the case of visualized pathology, the contribution of photooxidation and photodegradation products of bis-retinoids to the total fluorescence of the retinal pigment epithelium cell suspension increases in comparison with the norm...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726092/homeotic-mads-box-genes-encoding-lemads-mc-orthologues-in-wild-tomato-species-genus-solanum
#18
M A Slugina, E Z Kochieva, K G Skryabin, A V Shchennikova
New full-length genes encoding the LeMADS-MC transcription factor orthologues were identified and functionally characterized in wild tomato species S. cheesmaniae and S. habrochaites. A comparative analysis of the encoded proteins and LeMADS-MC of the cultivated tomato species S. lycopersicum revealed two major amino acid residues substitutions: V155E in the K-domain of ShaMADS-MC (S. habrochaites) and S80L in the I-region of SchMADS-MC (S. cheesmaniae). Structural differences of the C-terminal regions of MC and the canonical euAP1 proteins indicate possible chromosomal rearrangements in the Solanoideae subfamily, which, however, did not change the main known conserved euAP1 functions...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726091/modification-of-human-serum-albumin-under-induced-oxidation
#19
M G Gorobets, L A Wasserman, A D Vasilyeva, A V Bychkova, P G Pronkin, A E Bugrova, M I Indeykina, N G Shilkina, M L Konstantinova, A S Kononikhin, E N Nikolaev, M A Rosenfeld
For the first time, by using the complex of physicochemical methods (mass-spectrometry, differential scanning calorimetry, spectrofluorimetry, method of spectral and fluorescent probes, dynamic light scattering, and UV spectrophotometry), the oxidation-mediated modification of chemical and spatial structure of albumin has been studied. All albumin structural regions are subjected to oxidation, methionine and aromatic amino acids primarily involved in oxidation. The albumin melting shows a decrease in thermal stabilization of the structure and changing of aggregation upon oxidation...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726090/flavoprotein-minisog-bret-induced-cytotoxicity-depends-on-its-intracellular-localization
#20
E I Shramova, G M Proshkina, S M Deyev, R V Petrov
It is proposed to use the bioluminescent resonance energy transfer to solve the problem of creating the internal light sources in photodynamic therapy of cancer. Energy donor in the developed system is the oxidized form of the luciferase NanoLuc substrate furimamide, and acceptor is the phototoxic fluorescent protein miniSOG. It is shown that, in the proposed system, the photoinduced cytotoxicity of flavoprotein miniSOG in vitro depends on the intracellular localization, and the cytotoxic effect is 48% for the cytoplasmic localization of the fusion protein, 65% for the mitochondrial localization, and 69% for the membrane localization...
May 2017: Doklady. Biochemistry and Biophysics
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