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Doklady. Biochemistry and Biophysics

E S Meteleva, Yu S Chistyachenko, L P Suntsova, M A Tsyganov, G B Vishnivetskaya, D F Avgustinovich, M V Khvostov, N E Polyakov, T G Tolstikova, V A Mordvinov, A V Dushkin, N Z Lyakhov
The mechanochemical preparation of solid compositions of praziquantel with plant saponin (glycyrrhizic acid disodium salt) is described. The study of a number of physicochemical parameters showed that dissolving solid compositions in water is accompanied by the inclusion of praziquantel molecules into micelles, which are formed in the solution of the glycyrrhizic acid disodium salt. Using the opisthorchiasis model caused by Opisthorchis felineus, we found a 4- to 11-fold increase in the anthelmintic activity of praziquantel in the composition as compared to the official praziquantel...
July 2018: Doklady. Biochemistry and Biophysics
T A Gudasheva, P Yu Povarnina, T A Antipova, S B Seredenin
Dipeptide mimetic of the brain-derived neurotrophic factor bis(N-monosuccinyl-L-seryl-L-lysine) hexamethylenediamide (working name GSB-106), which reproduces the homodimeric structure of BDNF and the beta-turn of its fourth loop, activates TrkB, AKT, and ERK, exhibits neuroprotective and antidepressant activity, and is able to stimulate neurogenesis in the hippocamp of stressed mice. Using Western blot hybridization and synaptophysin (synaptogenesis marker), we showed the ability of chronically administered GSB-106 to stimulate synaptogenesis, increasing the synaptic density in the hippocamp by 50%...
July 2018: Doklady. Biochemistry and Biophysics
Z I Krutetskaya, L S Milenina, A A Naumova, S N Butov, V G Antonov, A D Nozdrachev
Using Fura-2AM microfluorimetry, we have shown for the first time that sigma-1 receptor agonist, tricyclic antidepressant amitriptyline, significantly inhibits glutoxim- and molixan-induced Ca2+ -responses in rat peritoneal macrophages. The results suggest possible involvement of sigma-1 receptors in the signaling cascade induced by glutoxim or molixan and leading to intracellular Ca2+ concentration increase in macrophages.
July 2018: Doklady. Biochemistry and Biophysics
M V Zinovyeva, L G Nikolaev, L G Kondratyeva, T V Vinogradova, E D Sverdlov
The mRNA content of the transcription factors KLF5 and ZEB1 was studied in pancreatic tumor tissues and in fetal and normal pancreas. Transcription of these factors was not high and similar in normal and fetal pancreatic tissues but greatly increased in the pancreatic ductal adenocarcinoma tissues. A significant positive correlation between the KLF5 and ZEB1 transcription levels in the pancreatic tumor tissues was observed.
July 2018: Doklady. Biochemistry and Biophysics
D A Gnatenko, E P Kopantzev, E D Sverdlov
Stimulation of BxPC-3, Panc-1, and MIA PaCA-2 pancreatic cancer cells with EGF, HGF, FGF-1, FGF-2, FGF-7, and FGF-10 growth factors caused changes in the expression of master genes regulating pancreatic development (SOX9, HNF3b, GATA-4, GATA-6, and HES1). This, in turn, caused changes in the expression profile of important transcription factors, embryonic development regulators. It was also found that the master genes belonging to the same family may cause opposite effects (suppression or enhancement of expression of a particular transcriptional regulator) in the same cell line...
July 2018: Doklady. Biochemistry and Biophysics
V N Petushkov, N S Rodionova
The first results of the study of chromatographic and spectral properties of the detected lowmolecular-weight activators, putative emitters in the luminescent reaction of Siberian enchytraeid Henlea sp., are presented.
July 2018: Doklady. Biochemistry and Biophysics
L G Kondratyeva, I P Chernov, M V Zinovyeva, V I Egorov, E P Kopantzev, E D Sverdlov
The expression levels of the SOX9 gene in fetal, postnatal, and neoplastic pancreatic tissues were compared. In the fetal pancreatic samples, the mean relative level of the SOX9 gene expression was 8 times greater than the normal level. The tumor samples were divided into three groups depending on the SOX9 expression level. The first group showed a 6.5-fold increased expression level of SOX9 with respect to the normal one. The second and normal groups had approximately equal levels expression. The third group showed a 25-fold decreased expression level of SOX9...
July 2018: Doklady. Biochemistry and Biophysics
I O Petrova, S A Tyganov, T M Mirzoev, A K Tsaturyan, I B Kozlovskaya, B S Shenkman
The study was aimed at testing the hypotheses about the role of cross-bridges and calpains in reduction of rat soleus passive tension under conditions of hindlimb unloading. For this purpose, we used an inhibitor of μ-calpain PD 150606 as well as a blocker of actomyosin interaction (blebbistatin). It was found for the first time that a decrease in passive tension of rat soleus after 3-day hindlimb unloading is associated with the activity of μ-calpain and does not depend on the processes of cross-bridges formation...
July 2018: Doklady. Biochemistry and Biophysics
E P Isakova, E G Serdyuk, N N Gessler, E V Trubnikova, Y K Biryukova, E Y Epova, Y I Deryabina, A V Nikolaev
A new recombinant strain of Yarrowia lipolytica synthesizing encapsulated highly thermostable phytase of Obesumbacterium proteus, which is recommended for use as a premix component of feed compositions in animal husbandry, was obtained.
July 2018: Doklady. Biochemistry and Biophysics
P A Kotelnikova, V O Shipunova, U F Aghayeva, O A Stremovskiy, M P Nikitin, I A Novikov, A A Schulga, S M Deyev, R V Petrov
A new method for obtaining biomodified magnetite nanoparticles for targeted delivery to cells was developed. The method is based on the use of the C-terminal fragment of the Mms6 protein, which is involved in the magnetite biomineralization during the synthesis of magnetosomes in magnetotactic bacteria Magnetospirillum magneticum AMB-1, and the barnase*barstar high-affinity protein pair. The Mms6 protein fragment is required for stabilizing magnetite, and the barnase*barstar pair mediates the interaction between nanoparticles and the component for modification...
July 2018: Doklady. Biochemistry and Biophysics
M V Yermokhin, N N Sushchik, V G Tabachishin, G S Kalacheva, A A Kolmakova, M I Gladyshev
The flow of long-chain polyunsaturated fatty acids (PUFAs) of the omega-3 family, namely, eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA), exported by amphibian metamorphs from water to terrestrial ecosystems in the Medveditsa River floodplain, was quantified for the first time. The total biomass export by three amphibian species (Pelobates fuscus, Bombina bombina, and Pelophylax ridibundus) per unit area of the lake surface was 0.594 g/m2 per year (as a mean for 2 years). The biomass flow per unit area of land was 0...
July 2018: Doklady. Biochemistry and Biophysics
A S Baik, K S Mironov, D V Arkhipov, M S Piotrovskii, E S Pojidaeva
The PepP protein has been purified in vitro and characterized for the first time. It is encoded by the sll0136 gene of the unicellular cyanobacterium Synechocystis sp. PCC6803. It is established that the PepP protein is a Mn2+ -dependent Xaa-Pro-specific aminopeptidase. The protein in the reaction of hydrolysis of the fluorescent peptide Lys(N-Abz)-Pro-Pro-pNA has a maximal activity at pH 7.6 and 32°C.
July 2018: Doklady. Biochemistry and Biophysics
O I Nedelyaeva, A V Shuvalov, O V Mayorova, A A Yurchenko, L G Popova, Yu V Balnokin, I V Karpichev
One of the genes of the CLC (Chloride Channel) family, SaCLCc1, from the halophyte Suaeda altissima (L.) Pall. was cloned. To investigate the function of SaCLCc1, it was expressed in the S. cerevisiae deletion mutant Δgef1::LEU2 for the only gene of the CLC family in this organism. The growth of the transformed SaCLCc1-expressing mutant Δgef1 was restored when cells were grown in Fe2+ -deficient YPEG medium, in minimal synthetic media SD and SR (pH 7.0), and in rich YPD medium containing Mn2+ . The complementation of the Δgef1 mutant phenotype with the SaClCc1 gene indicates the involvement of the SaClCc1 protein in the transport of Cl- ions...
July 2018: Doklady. Biochemistry and Biophysics
A Ya Bolsunovsky, D V Dementyev, E A Trofimova, E M Iniatkina, Yu V Kladko, M V Petrichenkov
The effect of γ-radiation on the cytogenetic parameters of root meristem cells of onion seedlings was studied in laboratory experiments (Allium-test). An increase in the overall frequency of chromosomal aberrations and micronucleus frequencies in seedling cells at low γ-radiation doses (≤0.1 Gy) was detected for the first time. At a maximum absorbed dose of 13 Gy, chromosomal aberrations were detected in the majority of cells in the anaphase and telophase stages of the cell cycle, and the number of cells with multiple aberrations increased...
July 2018: Doklady. Biochemistry and Biophysics
K V Purtov, A Yu Gorokhovatsky, A A Kotlobay, Z M Osipova, V N Petushkov, N S Rodionova, A S Tsarkova, T V Chepurnykh, I V Yampolsky, J I Gitelson
This is the first study to obtain a high-purity luciferase from the fungus Neonothopanus nambi biomass that is suitable for subsequent sequencing.
May 2018: Doklady. Biochemistry and Biophysics
A P Puzyr, S E Medvedeva, A E Burov, Yu P Zernov, V S Bondar
In in vitro experiments, the possibility of using a luminescent system extracted from the luminous fungus Armillaria borealis has been shown to detect and determine the concentration of hispidin. A linear dependence of the luminescent response on the content of hispidin in solutions in the concentration range of 5.4 × 10-5 -1.4 × 10-2 µM was detected. The stability of the enzyme system and the high sensitivity of the bioluminescent reaction allows carrying out multiple measurements with the analyte detection limit of 1...
May 2018: Doklady. Biochemistry and Biophysics
T S Lopatina, E S Zadereev, N A Oskina, M V Petrichenkov
We investigated the sensitivity of resting eggs of the cladoceran Moina macrocopa to the effect of ionizing radiation during the reactivation of the eggs. The study showed that the resting eggs during reactivation are more vulnerable to irradiation than the resting eggs in a stage of deep dormancy. The decrease in the efficiency of egg reactivation was observed at high doses, the growth rate of juveniles, fecundity, and the number of produced clutches by females strongly decreased when resting eggs at the reactivation stage absorbed doses of 64 Gy and higher...
May 2018: Doklady. Biochemistry and Biophysics
O G Maksimenko, E V Belova, P G Georgiev
The role of the gypsy insulator in the replication origin (RO) activity in the presence and absence of one and two copies of this insulator in several genomic sites was studied. Due to the fact that the prepared model system makes it possible to study the activity of this element in a given genomic site, it was shown that the RO stabilization, indeed, is determined by the activity of the insulator rather than by the construct integration site into the genome. The role of the Su(Hw) protein in this process was also studied in detail...
May 2018: Doklady. Biochemistry and Biophysics
Z I Krutetskaya, L S Milenina, A A Naumova, S N Butov, V G Antonov, A D Nozdrachev
Using Fura-2AM microfluorimetry, we have shown for the first time that preincubation of macrophages with sigma-1 receptor antagonist haloperidol leads to a significant inhibition of the store-dependent Ca2+ entry induced by endoplasmic Ca2+ -ATPase inhibitors thapsigargin or cyclopiazonic acid in rat peritoneal macrophages. The results suggest the involvement of the sigma-1 receptor in the regulation of storedependent Ca2+ entry in macrophages.
May 2018: Doklady. Biochemistry and Biophysics
I V Alekseenko, V V Pleshkan, A V Sass, O B Filyukova, E V Snezhkov, E D Sverdlov
An artificial double tandem tumor-specific promoter based on survivin and human telomerase reverse transcriptase gene promoters was constructed. Studies in in vitro and ex vivo therapeutic systems showed that the designed promoter exhibits a high activity in tumor cells, which is comparable to the activity of the CMV constitutive promoter.
May 2018: Doklady. Biochemistry and Biophysics
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