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Doklady. Biochemistry and Biophysics

M Yu Mazina, E V Kocheryzhkina, J V Nikolenko, A N Krasnov, S G Georgieva, N E Vorobyeva
We found that an increase in the expression level of E75, DHR3, and ERR increases the degree of activation of dhr3 and hr4 genes in Drosophila S2 cells. We also detected a repressing effect of these nuclear receptors on the basal transcription level of these genes. This is the first study to show the ability of nuclear receptors E75, DHR3, and ERR to function as activators or repressors depending on external conditions. We also confirmed the existence of the interaction of all studied nuclear receptors with the promoters of dhr3 and hr4 genes of the ecdysone cascade in vivo...
March 2017: Doklady. Biochemistry and Biophysics
E D Nikolskaya, O A Zhunina, N G Yabbarov, V I Shvets, B I Krugliy, E S Severin
A recombinant alpha-fetoprotein (rAFP) was obtained in the yeast P. pastoris system, and its functional activity was confirmed. A method for producing polymer particles loaded with dactinomycin was developed, and a conjugate of these nanoparticles with rAFP was synthesized. The efficiency of the obtained conjugate on the HeLa, SKOV3, and MG-63 tumor cells and the absence of toxicity on the normal cells was shown. Experiments in vivo demonstrated a significant increase in the antitumor efficacy of the conjugate at a lower general toxicity as compared to the commercially available dactinomycin...
March 2017: Doklady. Biochemistry and Biophysics
A A Rosenkranz, T A Slastnikova, Yu V Khramtsov, T S Karyagina, G P Georgiev, A S Sobolev
A new modular nanotransporter (MNT) for the delivery of anticancer agents into the nuclei of cells with folate receptor overexpression was created. An effective method for acceding labeling of this MNT with Auger electron emitter (111)In has been developed. A significant therapeutic effect was observed after a single intratumoral injection of the new (111)In-labeled MNT to mice grafted with human cervical carcinoma characterized by folate receptor overexpression.
March 2017: Doklady. Biochemistry and Biophysics
O E Dick, B V Krylov, A D Nozdrachev
The use of the mathematical model of rat nociceptive neuron membrane allowed us to predict a new mechanism of suppression of ectopic bursting discharges, which arise in neurons of dorsal root ganglia and are one of the causes of neuropathic pain. The treatment with comenic acid leads to switching off the ectopic bursting discharges due to a decrease in the effective charge transferring via the activation gating structure of the slow sodium channels (Na V1.8a). Comenic acid is a drug substance of a new non-opioid analgesic [1] Thus, this analgesic not only reduces the frequency of rhythmic discharges of nociceptive neuron membrane [2] but also it suppresses its ectopic bursting discharges...
March 2017: Doklady. Biochemistry and Biophysics
E Z Kochieva, M A Filyushin, A V Beletsky, N V Ravin, K G Skryabin
Genes encoding six chitinases, five of which belong to classes I (MhCHI3 and MhCHI4), IV (MhCHI1), V (MhCHI5), and VII (MhCHI2), were identified in the transcriptome of the parasitic mixoheterotrophic plant Monotropa hypopitys. The transcription level of MhCHI5 and MhCHI1 was low; however, in the leaves (bracts) and roots it was higher than in flowers. MhCHI4 transcripts were detected primarily in the flowers and were almost absent in the roots, whereas the expression level of MhCHI3 was relatively high in all organs but maximum in the leaves (bracts)...
March 2017: Doklady. Biochemistry and Biophysics
A V Kolesnikov, A V Kozyr, A K Ryabko, I G Shemyakin, I A Dyatlov
A model of a controlled conversion of polysaccharide Vi-antigen of S. typhi into zwitterionic antigen is proposed. The immunological properties of modifications of this antigen conjugated to a protein support were studied.
March 2017: Doklady. Biochemistry and Biophysics
N A Ushakova, A E Dontsov, A I Bastrakov, N Y Garmash, D S Pavlov
Larva, prepupa (last instar larva), pupa, and an empty shell of pupa after hatching of the black soldier fly Hermetia illucens contain eumelanin, an active synthesis of which is observed at the prepupal stage, which is probably due to the release of prepupa from the feed substrate thickness to the open space for pupation. It was shown for the first time that prepupa contains high quantities of the magnetically active form of manganese Mn(2+). This fact indicates that Mn(2+) stimulates the copper-containing tyrosinase-the key enzyme in the synthesis of melanin in the period of migration and adaptation of the insect to the solar radiation...
March 2017: Doklady. Biochemistry and Biophysics
V S Morozova, I A Moseikin, S N Petrochenko, M A Myagkova, L F Panchenko
Prognostic immuno-biochemical tests to assess the severity of the condition of patients with cardiac diseases were developed. A comparative analysis of clinical and laboratory blood parameters, which are used in practice for the diagnosis and prognosis of diseases, and an analysis of the content of natural antibodies (n-Abs) to β-endorphin, bradykinin, histamine, dopamine, and serotonin were performed. A significant correlation between the level of n-Abs, the main biochemical parameters, and the content of blood corpuscles was revealed...
March 2017: Doklady. Biochemistry and Biophysics
Z I Krutetskaya, L S Milenina, A A Naumova, S N Butov, V G Antonov, A D Nozdrachev
Using Fura-2AM microfluorimetry, we have shown for the first time that preincubation of macrophages with methyl-β-cyclodextrin, inducing cholesterol extraction from membranes and raft disruption, leads to significant inhibition of thapsigargin-induced store-dependent Ca(2+) entry in rat peritoneal macrophages. In contrast, macrophage treatment with methyl-β-cyclodextrin after Ca(2+) entry mechanisms were activated by store depletion by thapsigargin application leads to potentiation of subsequent store-dependent Ca(2+) entry...
March 2017: Doklady. Biochemistry and Biophysics
P P Knox, K N Timofeev, V V Gorokhov, N Kh Seifullina, A B Rubin
The study of the effect of vasodilator, antiplatelet agent, and inhibitor P-glycoprotein dipyridamole (DIP) on the functioning of the transmembrane protein of the reaction center (RC) of Rb. sphaeroides showed that the activation of RC by constant light generates the DIP radical cation, which significantly affects the kinetics of recombination of charges divided between photoactive bacteriochlorophyll and quinone acceptors. Thus, the antioxidant properties of DIP may affect the functional activity of membrane proteins, and this apparently should be taken into account in the studies of the mechanisms of therapeutic action of this drug...
March 2017: Doklady. Biochemistry and Biophysics
K V Shevchenko, I Yu Nagaev, V P Shevchenko, L A Andreeva, S I Shram, N F Myasoedov
It was shown that the neuroactive peptide 5-oxo-Pro-Arg-Pro (5-oxo-PRP) is detected in the brain in the time interval of 5-120 min after it was intravenously or intranasally administered to rats; the maximum concentration of labeled tripeptide in these modes of administration was observed after 30 and 10 min, respectively. A significant difference in the concentrations of 5-oxo-PRP in the blood and brain (the latter was 50 times lower) during intravenous administration indicates a relatively low permeability of the peptide across the blood-brain barrier...
March 2017: Doklady. Biochemistry and Biophysics
A K Golovnin, V V Molodina, I S Shapovalov, P G Georgiev, L S Melnikova
Earlier, we showed in a model system of the yellow gene of D. melanogaster that an increase in the EAST protein concentration leads to repression in bristles, the mechanism of which remained obscure. In this study, an inverted repeat was localized by genetic methods in the long terminal repeat (LTR) sequence of the MDG4 retrotransposon. This repeat includes the binding site for the Mad protein-the key component of the of TGF-β/BMP signaling cascade. The results of this work suggest that the Mad protein recruits to chromatin a regulatory complex that functionally interacts with the EAST protein...
March 2017: Doklady. Biochemistry and Biophysics
S V Prudnikova, O N Vinogradova, M Y Trusova
The study addresses the influence of the physicochemical properties of the reserve cellular macromolecules (polyhydroxyalkanoates, PHA) with different chemical composition on their biodegradation in the agro-transformed field soil of the Siberian region (Krasnoyarsk Territory, Russia). It was shown that the degradation of the PHA samples depends on the degree of polymer crystallinity (Cx). For the first time, it was shown that the range of PHA-degrading microorganisms differs for each of PHA types. The study defines the primary degraders specific to each PHA type and common to all types of examined polymers...
March 2017: Doklady. Biochemistry and Biophysics
O P Zhirnov, K A Akulich, A V Lipatova, E V Usachev
It was shown that full-length virion RNA of segment 8 of influenza virus A/Aichi/2/68 (H3N2) can initiate the synthesis of two major polypeptides with molecular weights of 23 and 13 kD and a minor polypeptide with a molecular weight of 19 kDa, which specifically reacted with the antibodies to the 30-membered peptide of the central part of the NSP protein of influenza A virus. Thus, the genomic-polarity RNA of segment 8 of influenza virus A has a translational template function. These data provide further confirmation of the concept of the bipolar (ambisens) strategy of functioning of the influenza A virus genome...
March 2017: Doklady. Biochemistry and Biophysics
T D Tatarinova, A A Perk, V V Bubyakina, I V Vasilieva, A G Ponomarev, T C Maximov
This is the first study to investigate stress proteins dehydrins with the use of specific antibodies in the Scots pine (Pinus sylvestris L.) needles and their changes in the annual cycle under extreme climate of Yakutia. No pronounced polymorphism of major dehydrins (14-15 and 66 kDa) has been found during the winter dormancy period of P. sylvestris. A clear correlation between the seasonal variations in dehydrins and changes in the water content in needles was revealed. Consistently high levels of dehydrins was retained throughout the period of low negative temperatures...
March 2017: Doklady. Biochemistry and Biophysics
D S Karlov, E V Radchenko, V A Palyulin, N S Zefirov
N-Methyl-D-aspartic acid (NMDA) receptor is a promising target for treatment of neurodegenerative diseases and other brain disorders as well as for designing proneurogenic compounds able to stimulate neurogenesis in adult brain. We analyzed the structure of the binding site of negative allosteric modulators in the amino-terminal domain of the NMDA receptor and identified possible modes of their binding as well as performed molecular design of new modulators that significantly differ from the known ones in structure and binding mode...
March 2017: Doklady. Biochemistry and Biophysics
E V Radchenko, Yu A Rulev, A Ya Safanyaev, V A Palyulin, N S Zefirov
The hERG potassium channel is one of the most important anti-targets determining cardiotoxicity of potential drugs. Using fragmental descriptors and artificial neural networks, the predictive models of the relationship between the structure of organic compounds and their activity with respect to hERG were built, and the structural factors affecting it were analyzed. By their predictive ability and applicability domain, these models (N = 1000, Q (2) = 0.77, RMSE cv = 0.45 for affinity and N = 2886, Q (2) = 0...
March 2017: Doklady. Biochemistry and Biophysics
V A Lakunina, I Y Petrushanko, K M Burnysheva, V A Mitkevich, A A Makarov
We have shown that the inhibition of Na,K-ATPase during its long-term incubation with amyloid beta (Aβ42), an Alzheimer's disease protein, is caused by the change in the thiol redox status of cells leading to induction of glutathionylation α-subunit of Na,K-ATPase. To restore the activity of Na,K-ATPase, it is proposed to use reducing agents, which promote normalization of the redox status of cells and deglutathionylation of the protein.
March 2017: Doklady. Biochemistry and Biophysics
D G Sochivko, A A Fedorov, Y I Alekseev, V E Kurochkin, M V Dubina
The course of the real-time polymerase chain reaction (PCR) is determined by the temperature dependence of the kinetics of the component reactions, particularly the DNA strand hybridization. To investigate the effect of thermal processes on the reaction behavior, a mathematical model in which the variable rate constant of dissociation of "primer-single strand" complexes depends on temperature was proposed. The reaction medium temperature, which depends on time, was also introduced into the model. The proposed model of real-time PCR makes it possible to analyze different aspects of the reaction, which are important for the development of instruments and reagents for PCR...
January 2017: Doklady. Biochemistry and Biophysics
L V Topchieva, I A Nilova, A F Titov
Under exposure of wheat plants to high temperatures (33, 37, and 43°C), the level of transcripts of the genes encoding proapoptotic proteins-TaMCAII (encodes type II metacaspase) and TaBAX (functional homologue of the CDF1 gene of Arabidopsis thaliana)-in leaf cells increased. This process at temperatures of 37 and 43°C passed ahead of (in the case of TaBAX mRNA) or was accompanied by (in the case of TaMCAII mRNA) the emergence of signs of nucleosomal DNA degradation in leaves. The accumulation of malondialdehyde in the leaves of seedlings exposed to temperatures of 37 and 43°C coincided in time with a change in the TaMCAII gene expression and the emergence of signs of programmed cell death...
January 2017: Doklady. Biochemistry and Biophysics
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