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Doklady. Biochemistry and Biophysics

V L Zamoyski, E A Vikhareva, V V Grigoriev, S O Bachurin
Using patch-clamp method (whole cell configuration), it was shown that tetraethylammonium (TEA) and 4-aminopyridine (4-AP) block calcium-dependent chloride currents in the membrane of freshly isolated cerebellar Purkinje cells of rats (12-15 days). In the concentration range studied (50 μM-10 mM TEA and 100 μM-1 mM 4-AP), both compounds blocked the chloride current at IC50 130 μM for TEA and 110 μM for 4-AP. TEA blockade was reversible after washing. The effect of 4-AP at concentrations greater than 100 μM was irreversible: both outward and inward chloride currents were blocked even after the removal of 4-AP from the incubation medium...
September 2016: Doklady. Biochemistry and Biophysics
A A Tikhomirov, S V Trifonov, E A Morozov, Yu A Kudenko, G S Kalacheva, S A Ushakova
Methods of physicochemical further oxidation of hardly soluble sediment obtained from "wet combustion" of human exometabolites applied to space-purpose Bio Technological Life Support Systems (BTLLS) were studied. Most hardly dissoluble sediment containing Ca, P, Mg, and other essential plant nutrition elements were shown to dissolve in H2O2 and HNO3 aqueous media activated by alternating electric current. Dissolved additional mineral elements allowed (as demonstrated for lettuce) to increase the productivity of BTLLS phototrophic unit plants more than twice, which is comparable to their productivity on standard Knop solution with balanced chemical composition...
September 2016: Doklady. Biochemistry and Biophysics
E R Mingazov, M V Ugrumov
Degeneration of dopaminergic neurons in the substantia nigra and the decrease in the dopamine level in the striatum lead to dysfunctions of motor behavior. This is accompanied by dysregulation of neuro-transmission in glutamatergic neurons of the motor cortex and GABA-ergic neurons of the striatum. It is shown that dysregulation of the gene expression of vesicle cycle proteins in neurons of the motor cortex occurs at an early (presymptomatic) stage of degeneration of the nigrostriatal system, and in more severe degeneration (symptomatic stage) the level of gene expression of vesicle cycle proteins in the striatum decreases...
September 2016: Doklady. Biochemistry and Biophysics
T M Mirzoev, Y N Lomonosova, O E Zinovyeva, E A Lysenko, B S Shenkman, T L Nemirovskaya
Intracellular signaling pathways were investigated in skeletal muscle cells at the early stages of alcohol addiction manifestations. No muscle fiber atrophy was observed in m. vastus lateralis of male patients. No significant changes in the signaling mechanisms that control protein degradation were detected as well. However, the concentration of the insulin-like growth factor (IGF-1) in blood plasma as well as the content of markers of intracellular signaling pathways regulating protein synthesis were significantly reduced compared to the control group...
September 2016: Doklady. Biochemistry and Biophysics
E V Kuevda, E A Gubareva, S V Krasheninnikov, T E Grigoriev, I S Gumenyuk, A S Sotnichenko, I V Gilevich, D D Karal-Ogly, S V Orlov, S N Chvalun, A N Redko, S N Alekseenko, P Macciarini
The effect of decellularization on the biomechanical properties of macaque lungs was studied. The quality of the biological scaffold was additionally assessed by morphological methods, and the contents of extracellular matrix (ECM) fibers were determined both qualitatively and quantitatively. Histological analysis revealed no damage of structural integrity of ECM components, but the scaffold elasticity significantly decreased, which was confirmed by the changes in the hysteresis loop without a concomitant decrease in peak loads, with the mechanical strength of the samples being retained...
September 2016: Doklady. Biochemistry and Biophysics
A A Fedorov, A S Berdnikov, D G Sochivko, D A Varlamov, V E Kurochkin, R V Petrov
Macroscopic kinetic models describing the process of polymerase chain reaction (PCR) are currently solved only by numerical methods, which hampers the development of effective software algorithms for processing the results of the reaction. This paper considers the application of the homotopy perturbation method for obtaining approximate analytical solution of the simplest system of enzymatic kinetic equations describing the synthesis of nucleic acid molecules during PCR. The resulting approximate analytic solution with high accuracy reproduces the results of a numerical solution of the system in a wide range of ratios of enzyme and substrate concentrations both for the case of a large excess of the substrate over the enzyme and vice versa...
September 2016: Doklady. Biochemistry and Biophysics
N V Lifantseva, Ts O Koneeva, S N Voronova, L A Zakharova, V I Melnikova
The mRNA for dopamine receptors of type D1, D3, D5, but not type D2, was detected in the thymus of rats starting from day 16 of embryonic development (E16). Dopamine at concentrations of 10(-8)-10(‒6) M inhibited fetus thymocyte response to mitogen, confirming the functionality of the receptors and the possibility of a direct effect of dopamine on the developing thymus. Pharmacological inhibition of catecholamine synthesis in the crucial period of thymus development leads to long-term changes in the T-system immunity due to increased production of natural regulatory T-lymphocytes...
September 2016: Doklady. Biochemistry and Biophysics
D V Tyulkina, V V Pleshkan, I V Alekseenko, M R Kopantseva, E D Sverdlov
The fibroblast activation protein (FAP) is selectively expressed in cancer-associated fibroblasts (CAFs) and facilitates tumor progression, which makes this protein an attractive therapeutic target. There are difficulties in obtaining CAFs for studying the function and suppression of FAP. In this work, the expression level of FAP was determined by PCR assay in 25 human cell lines and 8 surgical samples of tumor stroma. The expression of FAP was observed in all tumor stroma samples and in four cell lines: NGP-127, SJCRH30, SJSA-1, and A375...
September 2016: Doklady. Biochemistry and Biophysics
M V Lobanova, A Y Ratushnyy, L B Buravkova
The expression of several genes which functions are associated with cellular senescence was analyzed in multipotent mesenchymal stromal cells during long-term cultivation at different oxygen levels (20, 5, and 1%) using the RT² Profiler™ PCR Array Human Cellular Senescence system (Qiagen, United States). It was established that replicative senescence processes develop most actively in the cells cultured under the standard conditions (20% O2). The most significant changes were observed in the expression of CCND1, ID1, IGF1, PIK3CA, and SERPINE1 genes...
September 2016: Doklady. Biochemistry and Biophysics
I E Kasheverov, E V Kryukova, D S Kudryavtsev, I A Ivanov, N V Egorova, M N Zhmak, E N Spirova, I V Shelukhina, A V Odinokov, M V Alfimov, V I Tsetlin
We studies the receptor-binding specificity of the synthetic peptide HAP (High Affinity Peptide) and its analogues, which are regarded as a model of the orthosteric site nicotinic acetylcholine receptors (nAChR). Using radioligand analysis, electrophysiology tests, and calcium imaging, we assessed the ability of HAP to interact with nAChR antagonists: long α-neurotoxins and α-conotoxins. A high affinity of HAP for α-bungarotoxin and the absence of its interaction with α-cobratoxin and α-conotoxins was found...
September 2016: Doklady. Biochemistry and Biophysics
A G Sulkarnayeva, J N Valitova, F V Minibayeva
Three homeologous copies of the TaSMT1 gene for C24-sterol methyltransferase, which are located on chromosomes A, B, and D of Triticum aestivum hexaploid genome, were discovered. The bioinformatic analysis of the structure of these genes and sequencing de novo promoter sequences revealed differential expression of homeologous TaSMT1 genes in leaves and roots of wheat seedlings under normal conditions and in stress.
September 2016: Doklady. Biochemistry and Biophysics
I K Kolomiytseva, A A Lakhina, L N Markevich, E E Fesenko
The content of neutral lipids in tissue homogenates and liver cell nuclei of hibernating Yakutian ground squirrels was studied. In homogenates, hibernation increases the content of fatty acids and reduces the content of glycerides and cholesterol. When studying the liver cell nuclei of torpid winter ground squirrels, we detected a twofold increase in the content of fatty acids, cholesterol, and monoglycerides as compared to the "summer" ground squirrels. In the active "winter" ground squirrels, as compared to the torpid winter ones, the content of cholesterol did not change, whereas the content of fatty acids, monoglycerides, and diglycerides decreased but remained higher than in the "summer" ground squirrels...
September 2016: Doklady. Biochemistry and Biophysics
A S Dyabina, E V Radchenko, V A Palyulin, N S Zefirov
Using fragmental descriptors and artificial neural networks, a predictive model of the relationship between the structure of organic compounds and their blood-brain barrier permeability was constructed and the structural factors affecting the readiness of this penetration were analyzed. This model (N = 529, Q (2) = 0.82, RMSE cv = 0.32) surpasses the previously published models in terms of the prediction accuracy and the applicability domain and can be used for the optimization of the pharmacokinetic parameters during drug development...
September 2016: Doklady. Biochemistry and Biophysics
I V Zelepukin, M P Nikitin, V R Cherkasov, P I Nikitin, S M Deyev, P V Petrov
Magnetic markers which can be detected with an extremely high sensitivity with the method of magnetic particle quantification (MPQ) were synthesized. Using a controlled Stober reaction, a set of magnetic silica markers of different sizes and zeta potentials was obtained. The use of a carboxymethyl dextran polymer to stabilize the magnetite particles during the synthesis made it possible to substantially reduce the detection limit of the obtained construct, which opens up new opportunities for creating effective diagnostic nanoagents...
September 2016: Doklady. Biochemistry and Biophysics
I B Alchinova, S G Khaspekova, N V Golubeva, O N Shustova, O A Antonova, M Yu Karganov, A V Mazurov
Size of membrane microparticles (MPs) from blood plasma and MPs produced in vitro by activated endothelial cells (ECs), monocytes, THP-1 monocytic cells, granulocytes, and platelets was evaluated by dynamic light scattering. MPs were sedimented from the culture media, cell supernatants, and plasma at 20 000 g for 30 min. Average diameters of all types of MPs ranged from 300 to 600 nm. Plasma MPs had the smallest size. Close sizes were registered for MPs from platelets and THP-1 cells. MPs from monocytes were larger, and MPs from granulocytes and ECs were the largest ones...
September 2016: Doklady. Biochemistry and Biophysics
S A Zamorina, L S Litvinova, K A Yurova, N A Dunets, O G Khaziakhmatova, V P Timganova, M S Bochkova, P V Khramtsov, M B Rayev
The role of human pregnancy-specific β1-glycoprotein (PSG) in the regulation of expression of the transcription factor FOXP3 was studied. It is found that, under conditions of directed induction of phenotypic changes in CD4(+) lymphocytes to regulatory T cells (Treg), PSG at high concentrations (10 and 100 μg/mL) increased FOXP3 expression. The evaluation of the spontaneous expression level of FOXP3 mRNA showed that PSG (1 and 100 μg/mL) stimulated the expression of this factor in mononuclear cells and isolated CD4(+) lymphocytes...
September 2016: Doklady. Biochemistry and Biophysics
A V Goncharenko, N V Malyuchenko, A M Moisenovich, M S Kotlyarova, A Yu Arkhipova, A S Kon'kov, I I Agapov, A V Molochkov, M M Moisenovich, M P Kirpichnikov
3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds leads to an increase in the expression of alkaline phosphatase, an early marker of bone formation. Increased expression is associated with the actin cytoskeleton reorganization under the influence of 3D cultivation and osteogenic calcium phosphate component of the microcarrier.
September 2016: Doklady. Biochemistry and Biophysics
O A Bezborodova, E R Nemtsova, A R Gevorkov, A V Boyko, J B Venediktova, I V Alekseenko, M B Kostina, G S Monastyrskaya, E D Sverdlov, E V Khmelevskiy, R I Yakubovskaya
Antitumor efficacy of the combined suicide gene therapy and radiotherapy was studied on the model of CT26 murine colon adenocarcinoma. CMV-FCU1-IRES-mGM-CSF-pGL3 construct with PEG-PEI-TAT (FCU1-mGM/5-FC) block copolymer as a vector was used for intratumoral administration. Tumors were irradiated with a single 5 Gy dose. The efficacy was evaluated according to the grade of tumor growth inhibition (T/C) and lifespan of the animals. Pronounced antitumor activity of the combined use of FCU1-mGM/5-FC system with radiotherapy on the background of prolonged lifespan and the synergism of the applied methods was revealed...
September 2016: Doklady. Biochemistry and Biophysics
E V Novosadova, E S Manuilova, E L Arsenyeva, I A Grivennikov, N F Myasoedov
Induced pluripotent stem cells (iPSCs) can be a highly informative model of hereditary and sporadic human diseases. In the future, such cells can be used in substitution and regenerative therapy of a wide range of diseases and for the treatment of injuries and burns. The ability of iPSCs derived from patients with Parkinson's disease to differentiate into fibroblast-like cells (derivatives) was studied. It was found that these cells can serve as an effective feeder layer not only to maintain the pluripotency of allogenic and autologous iPSCs but also to derive new iPSC lines...
September 2016: Doklady. Biochemistry and Biophysics
F F Kulanbaewa, V Yu Sekova, E P Isakova, Y I Deryabina, A V Nikolaev
This article presents the characteristics of the highly inducible promoter of the gene encoding the mitochondrial porin, the voltage-dependent anion channel (VDAC). This promoter is recommended for use in new genetic constructs both in basic research for assessing the adaptive strategy of lower eukaryotes under adverse conditions and in designing new highly competitive transformants producing economically important compounds (proteins, lipids, and organic acids) on its basis.
September 2016: Doklady. Biochemistry and Biophysics
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