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Doklady. Biochemistry and Biophysics

A A Semenov, A G Enikeev, L V Snetkova, A V Permyakov, N A Sokolova, L V Dudareva
Optically active bis-2R(-)ethylhexyl o-phthalate was obtained with 0.18% yield from dry cultured cells of Aconitum baicalense Turcz ex Rapaics 1907 by extraction with petroleum ether followed by silica gel column chromatography. Its structure was confirmed by the analysis of (13)C and (1)H NMR spectra. Seasonal fluctuations of quantitative phthalate content in A. baicalense cells were identified. The tests were performed under conditions excluding the presence of phthalates in reagents, materials, and laboratory dishes...
November 2016: Doklady. Biochemistry and Biophysics
A A Fedorov, A S Berdnikov, D G Sochivko, D A Varlamov, V E Kurochkin, R V Petrov
Development of methods for obtaining approximate analytical solutions of nonlinear differential equations and their systems is a rapidly developing field of mathematical physics. Earlier, an approximate solution of the simplest system of kinetic enzymatic equations for calculating dynamics of complementary strands of nucleic acids was obtained. In this study, we consider an alternative approach to selecting the basic linear approximation of the used method, which makes it possible to obtain more accurate analytical solutions of the set problem...
November 2016: Doklady. Biochemistry and Biophysics
D A Dolgikh, A Yu Malyshev, M V Roshchin, G R Smirnova, O V Nekrasova, L E Petrovskaya, T B Feldman, P M Balaban, M P Kirpichnikov, M A Ostrovsky
Anion-selective opsins slow ChloC and ACR2 were expressed in rat brain cortical neurons by electroporation in utero. It is shown that the light-activated channel ACR2 has pronounced advantages in terms of both the inactivation kinetics and the neuron inhibition intensity, which is associated with a more negative value of the light-activated current reversal potential compared to the slow ChloC channel. The identified properties of opsin ACR2 indicate that it can be used for strictly controlled suppression of neuronal activity in optogenetic experiments, including the expression in the retinal ganglionic cells for reconstituting the OFF-component of their receptive field, which is essential for optogenetic prosthetics of degenerative retina...
November 2016: Doklady. Biochemistry and Biophysics
Z A Gabbasov, O S Saburova, O A Antonova, N V Golubeva, S G Khaspekova, O N Shustova, I T Zyuryaev, M Ya Ruda, A V Mazurov
Platelets bearing leukocyte antigen CD45 were identified in the blood of patients with myocardial infarction (MI) and healthy donors by flow cytofluorimetry. Part of these platelets contained tissue factor (TF)-primary initiator of blood clotting. The number of CD45(+) and CD45(+)/TF(+) platelets in MI patients at the first day was comparable with their level in healthy donors, but was increased at 8-12 days after MI onset. At that time in some patients the amount of CD45(+) and CD45(+)/TF(+) platelets reached 5-6 and 2-3% of their total number...
November 2016: Doklady. Biochemistry and Biophysics
I V Elanskaya, I A Kononova, E P Lukashev, Y V Bolychevtseva, M F Yanushin, I N Stadnichuk
In the large linker ArcE polypeptide of the phycobilisome (PBS) from the cyanobacterium Synechocystis sp. PCC 6803, the chromophore-containing 26-kDa domain was deleted with consequent disturbance of the main PBS functions. Phycobilisomes in mutant cells staying in contact with photosystem I cannot transfer energy to the photosystem II. Under the bright light conditions, the interaction of PBSs with the photoprotective orange carotenoid protein in the mutant was lost and the implementation of transition states 1 and 2 of the pigment apparatus was significantly reduced...
November 2016: Doklady. Biochemistry and Biophysics
Z I Krutetskaya, L S Milenina, A A Naumova, S N Butov, V G Antonov, A D Nozdrachev
Using Fura-2AM microfluorimetry, we have shown for the first time that methyl-β-cyclodextrin, inducing cholesterol extraction from membranes and raft disruption, significantly inhibits glutoxim- and molixan-induced Ca(2+)-responses in rat peritoneal macrophages. The results suggest that intact rafts are necessary for signaling cascade induced by glutoxim or molixan and leading to intracellular Ca(2+) concentration increase in macrophages.
November 2016: Doklady. Biochemistry and Biophysics
O V Andreenkov, E I Volkova, S A Demakov, X Xie, E B Dubrovsky, I F Zhimulev
For the first time we used a homologous recombination method to obtain complete and precise deletion of Drosophila dRNaseZ gene. In the founder line of flies in which the RNaseZ sequence was replaced by attP site, the full-length sequence of the gene was reintegrated, and its functionality was shown. This approach will allow us to generate further gene mutations in different domains of dRNaseZ protein and discover a broad spectrum and uncover functions outside of tRNA processing.
November 2016: Doklady. Biochemistry and Biophysics
D A Karagodin, N V Battulina, T I Merkulova, E M Baricheva
The regulatory region of the Trl gene was analyzed using the mutation Trl (3609) , resulting from the insertion of the P-element into the promoter region of the gene as well as mutations obtained on its basis. It is shown that two last transcription start sites, which are most often used in vitro in S2 cells, are almost not used in vivo. Experimental data indicate that transcription terminators in transposons play an important role in the decrease in the transcription level of the recipient gene.
November 2016: Doklady. Biochemistry and Biophysics
V M Olshanskii, V D Baron, Xue Wei
In 2-year-old Chinese giant salamanders Andrias davidianus, occasional electric discharges with a characteristic pattern similar to the electric discharges of weakly electric catfish, Polypterus and Protopterus, were recorded for the first time. The discharges markedly differ in shape from the myograms accompanying abrupt movements of the salamander or exceeded them in amplitude by more than an order of magnitude. The discharges were recorded both in the autonomous experiment in the absence of experimenters and at a weak tactile stimulation...
November 2016: Doklady. Biochemistry and Biophysics
M M Vorob'ev, N M Rao, K A Kochetkov
Proteolysis of β-lactoglobulin by trypsin was studied with fluorescence spectroscopy and an empirical exponential model was engaged to describe the peptide bond hydrolysis kinetics. The shift in the fluorescence maximum of tryptophan residues, from 342 to 352 nm, in the course of β-lactoglobulin degradation was used as an indicator of the transition of masked peptide bonds to the demasked ones, which were accessible for the enzyme action. A simple equation with only two parameters was suggested to link together the degree of demasking of peptide bonds and the degree of their hydrolysis, allowing the kinetic description of proteolysis...
November 2016: Doklady. Biochemistry and Biophysics
O N Shilova, E A Souslova, A M Pilunov, S M Deyev, R V Petrov
Development of agents for theranostics implies combining the targeting module, the effector module, and the detection module within the same complex or recombinant protein. We have constructed, isolated, and characterized the 4D5scFv-mCherry-PE(40) protein, which exhibits fluorescent properties and specifically binds to cancer cells expressing the HER2 receptor and reduces their viability. The ability of the obtained targeted antitumor agent 4D5scFv-mCherry-PE(40) to selectively stain the HER2-positive cells and its highly selective cytotoxicity against these cells make the obtained targeted recombinant protein 4D5scFv-mCherry-PE(40) a promising theranostic agent for the diagnostics and therapy of HER2-positive human tumors...
November 2016: Doklady. Biochemistry and Biophysics
M V Stanovova, P A Erokhov, N G Gornostaev, V S Mikhailov, Yu V Lyupina
We investigated functioning of proteasomes and chaperones in Arenicola marina coelomocytes in conditions of lipopolysaccharide-induced inflammation. We observed the increase of chymotrypsin-like proteasome activity in coelomocytes 1 h after induction. Amount of proteasome subunits alpha- and beta-5 types increased as well. We also detected appearance of a new form of Hsp70 chaperone in infected coelomocytes. Our results allow us to consider the changes in proteasome structure and induction of chaperones as principle mechanisms in stress adaptation and defensive reactions development in annelids...
November 2016: Doklady. Biochemistry and Biophysics
V V Lavrova, Zh V Udalova, E M Matveeva, F K Khasanov, S V Zinovieva
The dynamics of expression of two homologous genes Mi-1.1 and Mi-1.2 in the roots of resistant and susceptible tomato plants in non-invasion conditions and during invasion with the root-knot nematode M. incognita was studied. Nematode invasion was accompanied by a significant increase in the expression level of both genes; however, the accumulation of transcripts at the early stages of nematode invasion in the penetration of nematode juveniles to the roots was observed only in plants that contained the Mi-1...
November 2016: Doklady. Biochemistry and Biophysics
A M Marchenkov, A A Bondar, D P Petrova, K V Habudaev, Yu P Galachyants, Yu R Zakharova, N A Volokitina, M A Grachev
The existence of the cluster of duplicated sit silicon transporter genes in the chromosome of the diatom Synedra acus subsp. radians was shown for the first time. Earlier, the localization of sit genes in the same chromosome and cluster formation caused by gene duplication was shown only for the marine raphid pennate diatom P. tricornutum. Only non-clustered sit genes were found in the genomes of other diatoms. It is reasonable to assume that sit tandem (sit-td) and sit triplet (sit-tri) genes of S. acus subsp...
November 2016: Doklady. Biochemistry and Biophysics
V D Lakhno, A S Shigaev, T B Feldman, V A Nadtochenko, M A Ostrovsky
A quantum-classical model of photoisomerization of the visual pigment rhodopsin chromophore is proposed. At certain (and more realistic) parameter value combinations, the model is shown to accurately reproduce a number of independent experimental data on the photoreaction dynamics: the quantum yield, the time to reach the point of conical intersection of potential energy surfaces, the termination time of the evolution of quantum subsystem, as well as the characteristic low frequencies of retinal molecular lattice fluctuations during photoisomerization...
November 2016: Doklady. Biochemistry and Biophysics
E V Litasova, V V Iljin, A V Sokolov, V B Vasilyev, M A Dumpis, L B Piotrovskiy
It is shown for the first time that the mammalian enzymes can cause the degradation of the C60 fullerene molecules. This biodegradation is caused by the action of а hypochlorite generated neutrophil enzyme myeloperoxidase of fullerene molecule and leads to the loss of the topology of the fullerene core.
November 2016: Doklady. Biochemistry and Biophysics
M A Bolshakov, A A Ashikhmin, Z K Makhneva, A A Moskalenko
Carotenoid mixture enriched by rhodopin and spirilloxanthin was incorporated in LH2 and LH1 complexes from Allochromatium (Alc.) minutissimum in vitro. The maximum incorporating level was ~95%. Rhodopin (56.4%) and spirilloxanthin (13.8%) were incorporated into the LH1 complex, in contrast to the control complex, which contained primarily spirilloxanthin (66.8%). After incorporating, the LH2 complex contained rhodopin (66.7%) and didehydrorhodopin (14.6%), which was close to their content in the control (67...
November 2016: Doklady. Biochemistry and Biophysics
E N Sviriaeva, K V Korneev, M S Drutskaya, S A Nedospasov, D V Kuprash
The combined effect of innate immunity receptors in viral-bacterial coinfections was studied in vitro using the primary culture of murine macrophages activated by different combinations of ligands of innate immunity receptors belonging to the family of Toll-like receptors. The activation of macrophages first with a viral ligand and then with a bacterial one significantly decreased the expression of proinflammatory cytokine genes. Such attenuation of immune responses may occur during the development of bacterial complications in viral infections...
November 2016: Doklady. Biochemistry and Biophysics
M G Sharapov, R G Goncharov, A E Gordeeva, V I Novoselov, O A Antonova, A K Tikhaze, V Z Lankin
It is shown that endothelial cells from human umbilical vein have a reduced activity and gene expression of the "classic" antioxidant enzymes (Cu,Zn-superoxide dismutase, catalase, and Se-containing glutathione peroxidase). At the same time, a high expression level of peroxiredoxin genes was identified in the same endothelial cells, which obviously indicates the predominant involvement of these enzymes in protecting the endothelium from the damaging effect of free radical peroxidation.
November 2016: Doklady. Biochemistry and Biophysics
T A Gudasheva, V V Grigoriev, K N Koliasnikova, V L Zamoyski, S B Seredenin
We have previously shown that neuropeptide cycloprolylglycine (CPG) increases the content of brain-derived neurotrophic factor (BDNF) in the culture of neuronal cells under normal conditions and in pathology. This is the first study to show that CPG at a physiological concentration of 10(-6) M significantly enhances the transmembrane AMPA currents in rat cerebellar Purkinje cells. Thus, CPG is a positive endogenous modulator of AMPA receptors. It was assumed that the neuropsychotropic effects of CPG are implemented as a result of BDNF accumulation after the activation of AMPA receptors by this neuropeptide...
November 2016: Doklady. Biochemistry and Biophysics
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