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Doklady. Biochemistry and Biophysics

K V Purtov, A Yu Gorokhovatsky, A A Kotlobay, Z M Osipova, V N Petushkov, N S Rodionova, A S Tsarkova, T V Chepurnykh, I V Yampolsky, J I Gitelson
This is the first study to obtain a high-purity luciferase from the fungus Neonothopanus nambi biomass that is suitable for subsequent sequencing.
May 2018: Doklady. Biochemistry and Biophysics
A P Puzyr, S E Medvedeva, A E Burov, Yu P Zernov, V S Bondar
In in vitro experiments, the possibility of using a luminescent system extracted from the luminous fungus Armillaria borealis has been shown to detect and determine the concentration of hispidin. A linear dependence of the luminescent response on the content of hispidin in solutions in the concentration range of 5.4 × 10-5 -1.4 × 10-2 µM was detected. The stability of the enzyme system and the high sensitivity of the bioluminescent reaction allows carrying out multiple measurements with the analyte detection limit of 1...
May 2018: Doklady. Biochemistry and Biophysics
T S Lopatina, E S Zadereev, N A Oskina, M V Petrichenkov
We investigated the sensitivity of resting eggs of the cladoceran Moina macrocopa to the effect of ionizing radiation during the reactivation of the eggs. The study showed that the resting eggs during reactivation are more vulnerable to irradiation than the resting eggs in a stage of deep dormancy. The decrease in the efficiency of egg reactivation was observed at high doses, the growth rate of juveniles, fecundity, and the number of produced clutches by females strongly decreased when resting eggs at the reactivation stage absorbed doses of 64 Gy and higher...
May 2018: Doklady. Biochemistry and Biophysics
O G Maksimenko, E V Belova, P G Georgiev
The role of the gypsy insulator in the replication origin (RO) activity in the presence and absence of one and two copies of this insulator in several genomic sites was studied. Due to the fact that the prepared model system makes it possible to study the activity of this element in a given genomic site, it was shown that the RO stabilization, indeed, is determined by the activity of the insulator rather than by the construct integration site into the genome. The role of the Su(Hw) protein in this process was also studied in detail...
May 2018: Doklady. Biochemistry and Biophysics
Z I Krutetskaya, L S Milenina, A A Naumova, S N Butov, V G Antonov, A D Nozdrachev
Using Fura-2AM microfluorimetry, we have shown for the first time that preincubation of macrophages with sigma-1 receptor antagonist haloperidol leads to a significant inhibition of the store-dependent Ca2+ entry induced by endoplasmic Ca2+ -ATPase inhibitors thapsigargin or cyclopiazonic acid in rat peritoneal macrophages. The results suggest the involvement of the sigma-1 receptor in the regulation of storedependent Ca2+ entry in macrophages.
May 2018: Doklady. Biochemistry and Biophysics
I V Alekseenko, V V Pleshkan, A V Sass, O B Filyukova, E V Snezhkov, E D Sverdlov
An artificial double tandem tumor-specific promoter based on survivin and human telomerase reverse transcriptase gene promoters was constructed. Studies in in vitro and ex vivo therapeutic systems showed that the designed promoter exhibits a high activity in tumor cells, which is comparable to the activity of the CMV constitutive promoter.
May 2018: Doklady. Biochemistry and Biophysics
O G Maksimenko, E V Belova, P G Georgiev
The interaction of the Drosophila ENY2 protein with the ORC complex subunits was investigated. It is found that ORC4 and ORC6 subunits directly interact with ENY2.
May 2018: Doklady. Biochemistry and Biophysics
Z I Krutetskaya, A V Melnitskaya, V G Antonov, A D Nozdrachev
Using voltage-clamp technique, the involvement of epoxygenases in immunomodulatory drug glutoxim regulation of Na+ transport in frog skin was investigated. We have shown for the first time that preincubation of the frog skin with epoxygenase inhibitors econazole or proadifen almost completely inhibits the stimulatory effect of glutoxim on Na+ transport. The data suggest the involvement of the enzymes and/or products of epoxygenase oxidation pathway of arachidonic acid metabolism in glutoxim effect on Na+ transport in frog skin epithelium...
May 2018: Doklady. Biochemistry and Biophysics
A G Degermendzhi, A I Abakumov
The validity of the competitive exclusion principle (the Gause's principle) at one metabolic regulation factor is demonstrated for a general model of two-species community. The competitive exclusion principle postulates that a long-term coexistence of species is impossible if their number exceeds the number of density-dependent growth-regulating factors. Previously, this principle was proved for the stationary states in a general model of a community with any number of factors. In the dynamic modes, the number of species in a community may exceed the number of regulating factors...
May 2018: Doklady. Biochemistry and Biophysics
M G Gorobets, L A Wasserman, A V Bychkova, M L Konstantinova, I G Plaschina, M A Rosenfeld
For the first time, with the aid of differential scanning calorimetry, the thermal denaturation of fibrinogen under induced oxidation was studied. All fibrinogen structural elements detected by DSC (D region, αC-domain, and E region) are subjected to oxidation. Structural changes in fibrinogen molecule were characterized by the denaturation temperature, denaturation enthalpy, and van't Hoff enthalpy.
May 2018: Doklady. Biochemistry and Biophysics
V G Lebedev, A V Korobova, G V Shendel, G R Kudoyarova, K A Shestibratov
The effects of transformation of downy birch (Betula pubescens Ehrh.) with the GS1 gene encoding the cytosolic form of glutamine synthetase on the rooting of plants in vitro was studied. The transgenic plants had an elevated content of glutamine as well as glutamic and aspartic acids and rooted more rapidly than the control plants. Rooting on a medium containing the glutamine synthetase inhibitor phosphinothricin prevented the accumulation of auxin in birch plants carrying the GS1 gene, indicating the involvement of this enzyme in raising the level of auxins in the transgenic plants...
May 2018: Doklady. Biochemistry and Biophysics
Y Y Toporkova, E K Bessolitsyna, E O Smirnova, S S Gorina, O E Petrova, L S Mukhtarova, A N Grechkin
Data on the influence of the double bond geometry on the antimicrobial properties of different isomers of etherolenic acid against phytopathogenic bacteria are presented. (ω5Z)-Etherolenic acid possesses bactericidal properties against Xanthomonas campestris ssp. vesicatoria, Pseudomonas syringae ssp. tomato, Pectobacterium atrosepticum SCRI1043; the etherolenic and (11Z)-etherolenic acids possess only bacteriostatic properties.
May 2018: Doklady. Biochemistry and Biophysics
O A Mogilnaya, N O Ronzhin, K S Artemenko, V S Bondar
A bifunctional indicating complex was created by immobilization of extracellular oxidases (glucose oxidase and peroxidases) of luminous fungus Neonothopanus nambi onto modified nanodiamonds (MNDs) synthesized by detonation. It was found that the enzymes firmly adsorb onto MND particles and exhibit their catalytic activity. Model in vitro experiments showed that the created MND-enzymes complex is suitable for repeated use for analyte (glucose and phenol) testing and retains its activity after storage at 4°C in deionized water for 1 month...
May 2018: Doklady. Biochemistry and Biophysics
Yu P Kuznetsova, M V Goltyaev, O S Gorbacheva, S V Novoselov, E G Varlamova, E E Fesenko
The sodium selenite concentration that reduces the viability of Du-145 human prostate adenocarcinoma cells and F-9 mouse testicular teratocarcinoma cells was determined. We investigated the effect of sodium selenite on the mRNA expression level of the genes encoding mammalian selenocysteine-containing glutathione peroxidases and thioredoxin reductases (key antioxidant enzymes involved in the regulation of intracellular thiol redox balance), endoplasmic reticulum selenoproteins, and selenoproteins located in the testes and prostate...
May 2018: Doklady. Biochemistry and Biophysics
Ya V Makarova, E V Kryukova, I V Shelukhina, D S Lebedev, T V Andreeva, D Yu Ryazantsev, S V Balandin, T V Ovchinnikova, V I Tsetlin, Yu N Utkin
Genes encoding two three-finger toxins TFT-AF and TFT-VN, nucleotide sequences of which were earlier determined by cloning cDNA from venom glands of vipers Azemiops feae and Vipera nikolskii, respectively, were expressed for the first time in E. coli cells. The biological activity of these toxins was studied by electrophysiological techniques, calcium imaging, and radioligand analysis. It was shown for the first time that viper three-finger toxins are antagonists of nicotinic acetylcholine receptors of neuronal and muscle type...
March 2018: Doklady. Biochemistry and Biophysics
A I Kurtova, L K Dil'mukhametova, E R Mingazov, M V Ugrumov
In the striatum of rats at different stages of development, we determined the content of tyrosine hydroxylase and aromatic L-amino acids decarboxylase using double immunohistochemical labeling and estimated the expression level of their transcripts by real-time PCR. We found that, in different periods of development, there are three sources of dopamine in the striatum of rats: bienzymatic nerve fibers (throughout ontogeny), bienzymatic neurons (appear on day 18 of embryonic development), and monoenzymatic neurons (in adult animals)...
March 2018: Doklady. Biochemistry and Biophysics
V N Danilevich, V V Sorokin, Ya P Moiseev, S V Sizova
The patterns of formation of RNA nanoparticles (NPs) during thermal cycling of bacterial total tRNA in the presence of cations Ca2+ , Mn2+ , Ni2+ , Zn2+ , Co2+ , and Cu2+ were studied. The optimal conditions for the production of NPs were found, and it was revealed that their size depends on the ratio of the concentrations of Me2+ and tRNA. The concentration of reagents for obtaining NPs of small size (from 5 to 100 nm) was selected. It was shown that tRNA-based nanoparticles can comprise short (20-50 nt) ribooligonucleotides, including aptamers and siRNAs...
March 2018: Doklady. Biochemistry and Biophysics
V A Khoroshko, T Yu Zykova, O O Popova, I F Zhimulev
The precise genomic localization of the borders of 62 intercalary heterochromatin bands in Drosophila polytene chromosomes was determined. A new type of bands containing chromatin of different states was identified. This type is a combination of the gray band and the intercalary heterochromatin band, creating a genetic structure that with a light microscope is identified as a continuous band. The border structure of such bands includes the coding regions of genes with ubiquitous activity.
March 2018: Doklady. Biochemistry and Biophysics
U G Letuta, D M Shailina
The combined effect of the zinc magnetic isotope 67 Zn and weak magnetic field 25-35 mT causes a 2-4-fold increase in the colony-forming ability of bacteria E. coli in comparison with the nonmagnetic isotopes 64, 66 Zn. The effects of magnetic field in the range of 2.2-8 mT were detected for all bacteria regardless of the zinc-isotope enrichment of the media. This indicates the sensitivity of intracellular processes to weak magnetic fields. An increase in the ATP concentration in E. coli cells was only detected for the bacteria grown on the medium with the magnetic zinc isotope in the range of 2...
March 2018: Doklady. Biochemistry and Biophysics
N N Dygalo, T S Kalinina, D A Lanshakov
Irradiation of a mixture of oligonucleotides with dextran resulted in the formation of a complex that is detected by a decelerated migration of an irradiated sample in electrophoretic gel compared to a non-irradiated one. When injected into the brain of neonatal rats, the formed complex penetrated into the cells 3 times easier compared to the original oligonucleotide, thus indicating that the use of radiation crosslinking of oligonucleotides with oligosaccharides is promising to enhance the efficiency of delivery of gene-targeted oligonucleotide drugs into cells...
March 2018: Doklady. Biochemistry and Biophysics
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