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Polish Journal of Veterinary Sciences

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https://www.readbyqxmd.com/read/29166288/spirulina-platensis-affects-factors-involved-in-spermatogenesis-and-increases-ghrelin-receptors-in-testis-tissue-of-rats-fed-a-high-fat-diet
#1
O B B Esener, E Gurel-Gurevin, B Isbilen-Basok, F Yigit, T Bilal, A Altiner, N Yilmazer, E I Armutak
Ghrelin is a peptide hormone which plays important role in maintaining growth hormone release and energy homeostasis in vertebrates. Spirulina platensis (SP) has antioxidant and hypolipidemic effects due to its ingredients. In this study we aimed to investigate the effects of SP on the testicular structure and relation between ghrelin and testosterone in the testis of rats fed a high fat diet (HFD). Sixty four young adult male rats were used and divided to 8 equal groups. Experimental groups received addition of 10% cholesterol (CHL), 43% hydrogenated vegetable oil (HVO) and 3% SP alone or in combination to basal diet while the control group received only basal diet...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166287/assessment-of-genomic-inbreeding-in-polish-konik-horses
#2
S Kamiński, D M Hering, Z Jaworski, T Zabolewicz, A Ruść
The aim of this study was to assess the inbreeding coefficient of Polish Konik horses based on runs of homozygosity (ROH). Ninety six horses kept in 6 herds located across Poland were genotyped with the use of EquineSNP60 BeadChip (Illumina). SNP markers with a Minor Allele Frequency lower than 0.01 and SNPs assigned to chromosome X or Y were excluded from the study. A total of 50 708 SNPs were included for statistical analysis (SVS software, Golden Helix). The analysis showed that the population is in genetic equilibrium, with He and Ho estimates both equal to 0...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166286/a-trial-of-doramectin-injection-and-ivermectin-spot-on-for-treatment-of-rabbits-artificially-infested-with-the-ear-mite-psoroptes-cuniculi
#3
N M Elhawary, Sh S G H Sorour, M A El-Abasy, E K Bazh, K Sultan
The ear mite "Psoroptes cuniculi" is the main cause of ear mange, a highly contagious parasitic skin disease in rabbits all over the world. In the current work, a preliminary therapeutic trial to study the effect of the broad use acaricides doramectin and ivermectin on P. cuniculi was performed on artificially infested rabbits. Twenty five adult New Zealand white rabbits were used in this study. The rabbits were assigned randomly into five groups/ 5 rabbits in each group. Each rabbit was experimentally infested with 100 mites/ ear...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166285/an-evaluation-of-the-usefulness-of-invasive-and-non-invasive-methods-used-to-diagnose-helicobacter-spp-infections-in-dogs
#4
M Jankowski, J Spużak, K Kubiak, K Glińska-Suchocka, M Biernat
The aim of this study was to assess the suitability of invasive and non-invasive methods used to diagnose Helicobacter spp. in the stomachs of dogs. The study was carried out on 30 dogs of both sexes and different breeds, between one and 15 years old. A histopathologic examination, a microbiological culture, a rapid urease test, a direct bacteriological preparation and a nested PCR assay were carried out. Gastric Helicobacter spp. was identified in gastric biopsy specimens from 16 (53.3%) dogs using direct bacteriological preparation, in four (13...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166284/pulmonary-adiaspiromycosis-in-the-eurasian-beaver-castor-fiber-inhabiting-poland
#5
I Dolka, A Giżejewska, Z Giżejewski, J Kołodziejska-Lesisz, W Kluciński
Adiaspiromycosis is a rare fungal infection caused by saprophytic fungi Emmonsia spp. (type Ascomycota) occurring especially in small free-living mammals. The aim of this study was to evaluate the occurrence of histopathological lesions asscociated with adiaspiromycosis in the Eurasian beaver inhabiting Poland. In order to evaluate the presence of natural adiaspiromycosis we systematically investigated beaver populations from north-eastern Poland for adiaspores in the lungs. This study reveals for the first time the presence of pulmonary adiaspiromycosis of Eurasian beaver in Poland...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166283/cardiac-disorders-in-dogs-infected-with-babesia-canis
#6
M Bartnicki, P Łyp, P Dębiak, M Staniec, S Winiarczyk, K Buczek, Ł Adaszek
The aim of this study was to assess cardiac disorders in dogs infected with B. canis. The study included 50 dogs with babesiosis and 20 healthy control animals. All the animals had haematological tests, ECG, echocardiography and serum troponin I and CK-MB levels checked. The haematology in the group of dogs with babesiosis confirmed thrombocytopaenia in 100% of dogs, decreased haematocrit in 52% and anaemia in 46%. The most common abnormalities in ECG and echocardiography in dogs infected with protozoa included: change in appearance and/or amplitude of the T-wave (34%), increased fractional shortening (24%), an increased sinus rhythm (14%) and heart axis deviation (10%)...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166282/adenocarcinoma-of-the-posterior-segment-of-the-gastrointestinal-tract-in-dogs-clinical-endoscopic-histopathological-and-immunohistochemical-findings
#7
J Spużak, R Ciaputa, K Kubiak, M Jankowski, K Glińska-Suchocka, D Poradowski, M Nowak
Of all the tumours in dogs, three percent are located in the intestines, and 36-60% of those tumours affect the large intestine. Adenocarcinomas of the intestines account for 20-35% of the gastrointestinal tumours and for almost 60% of the large intestine tumours. The aim of the study was to analyze clinical disorders and endoscopic, histopathological and immunohistochemical changes in colorectal adenocarcinomas in dogs with the use of the E-cadherin, β-catenin, cytokeratin 20 (CK20), Ki-67 and minichromosome maintenance 3 (MCM-3)...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166281/epidemiological-study-of-canine-mast-cell-tumours-according-to-the-histological-malignancy-grade
#8
Anna Śmiech, B Ślaska, W Łopuszyński, A Jasik, M Szczepanik, P Wilkołek
The aim of the study was to identify significant relationships between the tumour malignancy grade and dogs' age, breed, sex, size, and location of mast cell tumours (MCTs). MCTs accounted for 13.27% of all diagnosed canine skin tumours. The highest incidence was recorded among Boxers, Labrador Retrievers, American Staffordshire Terriers, and Golden Retrievers. Statistical analysis revealed significantly higher probability of occurrence of the grade I mast cell tumour in the French Bulldog in the head, neck, torso, and limb regions, the grade-II mast cell tumour in Boxer, Doberman, Dachshund, shepherds, and setters in the scrotal region, and the grade III mast cell tumour in Shar-Pei in the axilla region...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166280/differentiation-of-infectious-bronchitis-virus-vaccine-strains-ma5-and-4-91-by-taqman-real-time-pcr
#9
T Stenzel, D Dziewulska, M Śmiałek, B Tykałowski, J Kowalczyk, A Koncicki
The aim of this study was to develop rapid molecular assays for differentiating vaccine strains Ma5 and 4/91 of the infectious bronchitis virus (IBV). Specific primers and probes for S1 and N genes were designed based on the nucleotide sequences of both vaccine strains. Cross-reactivity was not observed. Assay sensitivity was 2.373 × 103 copies of the Ma5 strain, and 3.852 x 103 copies of the 4/91 strain. Samples belonging to a known genotype demonstrated that the designed assays supported rapid and sensitive detection of Ma5 and 4/91 vaccine strains of IBV...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166279/influence-of-long-term-oral-application-of-quinolones-on-the-ecg-curve-in-dogs
#10
A Cepiel, A Noszczyk-Nowak, A Cekiera, J Popiel, U Pasławska
The aim of the study was to analyse the influence of enrofloxacin and pradofloxacin administered orally for 14 days on the ECG in dogs. The ECG was performed before and after a 14 day period of quinolone administration. There was an increase in the QTc and the TpTe interval in the group treated with quinolones. QTc was prolonged by 24 ms (p=0.001). The TpTe interval was shortened, on average, by 6.55 ms (p=0.048). In the group treated with enrofloxacin, QTc was prolonged by 16.27 ms (p=0.006) and the TpTe interval was shortened by 9...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166278/interactions-between-plant-derived-oestrogenic-substances-and-the-mycoestrogen-zearalenone-in-a-bioassay-with-mcf-7-cells
#11
Sabine Hessenberger, K Botzi, C Degrassi, P Kovalsky, C Schwab, D Schatzmayr, G Schatzmayr, J Fink-Gremmels
Human and animal diets may contain several non-steroidal oestrogenic compounds which originate either from plants (phytoestrogens) or from fungi that infect plants (mycoestrogens such as zearalenone (ZEN)). Phytoestrogens may compete with ZEN in binding to the oestrogen receptor β and thereby may counteract the oestrogenic activity of ZEN. Using a modified version of the E-screen assay, plant-derived oestrogenic substances were tested for their proliferative or anti-proliferative effect on oestrogen-dependent MCF-7 cells...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166277/the-influence-of-doxazosin-on-the-contractility-of-the-urinary-bladder-in-female-pigs-with-experimentally-induced-cystitis
#12
W Markiewicz, A Bossowska, E Lepiarczyk, M Majewski, P Radziszewski, A Wiśniewska, J J Jaroszewski
The present in vitro study investigated the influence of doxazosin on the contractility of the urinary bladder in female pigs with experimentally induced cystitis. Fifteen juvenile female piglets (18-20 kg body weight) were randomly assigned into three groups (n=5 animals each): i) control (clinically healthy animals, without doxazosin treatment), ii) animals with induced inflammation of the urinary bladder, but without doxazosin treatment (experimental group I) and iii) animals with inflamed bladder, treated orally with doxazosin (0...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166276/comparison-of-body-surface-area-based-and-weight-based-dosing-format-for-oral-prednisolone-administration-in-small-and-large-breed-dogs
#13
A Nam, S M Kim, J W Jeong, K H Song, T S Koo, K W Seo
This study compared the pharmacokinetics of Prednisolone (PDS) in small- and large breed dogs with a dosing format based on body surface area (BSA) or body weight (BW). The maximum concentration and area under the curve in large-breed dogs orally administered 2 mg/kg PDS were significantly greater than those in small-breed dogs given 2 mg/kg and in large-breed dogs given 40 mg/m2. The higher blood concentrations that result from BW-based dosing of oral PDS in large-breed dogs can be more than required for effect...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166275/plasma-profile-of-cimicoxib-in-sheep-after-oral-administration-at-two-different-rates
#14
A Di Salvo, M Giorgi, H K Lee, C Vercelli, F Rueca, M Trabalza Marinucci, G Della Rocca
Sheep are often subjected to painful procedures and thus they need to be treated with analgesics. Nevertheless, knowledges about pharmacokinetic features of these drugs in this species are poor. The aim of this study was to evaluate plasma behaviour of cimicoxib in sheep after a single oral administration at two different dose rates (4 and 6 mg/kg). Maximum plasma concentrations of cimicoxib were equal to 273.78 (median value; range 189.00-567.32) and 565.01 (range 308.27-822.59) ng/mL after treatment with 4 and 6 mg/kg, respectively...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166274/detection-of-experimental-swine-trichinellosis-using-commercial-elisa-test
#15
Michal Gondek, J Bień, Z Nowakowski
The aim of the study carried out on ten young (10-week old) pigs of the native Polish Large White breed experimentally infected with a low dose of 300 invasive muscle larvae (ML) of Trichinella spiralis was intravital detection of trichinellosis using the E-S ELISA test, determination of a variation level of IgG antibodies against excretory-secretory (E-S) antigens of T. spiralis muscle larvae and finally, describing the intensity of T. spiralis larvae infection in selected muscles. The pig sera were collected at 7 and 9 days prior to the experimental infection with T...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166273/first-report-of-feline-calicivirus-fcv-infection-in-stray-cats-in-northeast-china
#16
Kai Wang, Zhihua Pei, Guixue Hu
To improve our understanding of Feline calicivirus (FCV) infection in cats in Northeast China, 1584 serum samples from 974 domestic cats and 610 stray cats were collected between 2012 and 2015. The samples were tested for FCV antibodies using a commercially available ELISA kit. The results revealed an overall seroprevalence of 37.56% (595/1584), a seroprevalence in domestic cats of 32.85% (320/974) and a seroprevalence in stray cats of 45.08% (275/610). Risk factor analysis indicated that species was the only risk factor for the presence of FCV (OR=1...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166272/in-vitro-activity-of-some-essential-oils-alone-and-in-combination-against-the-fish-pathogen-nocardia-seriolae
#17
T F Ismail, T Yoshida
Microplate resazurin assay was applied to investigate the in vitro activity of four essential oils (EOs); cinnamon (Cinnamomum zeylanicum), thyme (Thymus vulgaris), lemongrass (Cymbopogon flexuosus) and tea tree (Melaleuca alternifolia) oils against 80 clinical isolates of the fish pathogen Nocardia seriolae. The checkerboard test was then used to determine the possible synergistic effect of EOs combination against reference type strains of fish nocardiosis. All tested EOs had antibacterial activity against N...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166271/beta-hydroxybutyrate-in-milk-as-screening-test-for-subclinical-ketosis-in-dairy-cows
#18
J Ježek, M R Cincović, M Nemec, B Belić, R Djoković, M Klinkon, J Starič
Ketosis is a very frequent metabolic disease in dairy cows, resulting in lower milk production, impaired fertility and increased frequency of other diseases. The course of the disease is often subclinical, so early detection is very important. The aim of the study was to investigate the relation between the concentration of beta-hydroxybutyrate in blood and milk and to determine the cut-off value in milk for detection of subclinical ketosis. The study included 94 cows, which were in the first third of lactation...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166270/detection-of-yersinia-enterocolitica-species-in-pig-tonsils-and-raw-pork-meat-by-the-real-time-pcr-and-culture-methods
#19
M A Stachelska
The aim of the present study was to establish a rapid and accurate real-time PCR method to detect pathogenic Yersinia enterocolitica in pork. Yersinia enterocolitica is considered to be a crucial zoonosis, which can provoke diseases both in humans and animals. The classical culture methods designated to detect Y. enterocolitica species in food matrices are often very time-consuming. The chromosomal locus _tag CH49_3099 gene, that appears in pathogenic Y. enterocolitica strains, was applied as DNA target for the 5' nuclease PCR protocol...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166269/selected-steps-of-swine-icsi-method-to-overcome-seasonal-effect-and-achieve-acceptable-early-embryonic-development-a-preliminary-study
#20
I A Tsakmakidis, M Tsantarliotou, I A Michos, A G Basioura, G Tsousis, C M Boscos
Porcine intra cytoplasmic sperm injection's (ICSI) efficacy by selected protocol steps was investigated. Three trials per year's period (hot, medium, cold) were carried out. Only large size follicles (6-8mm) were aspirated, brilliant cresyl blue (BCB) test was performed and only the BCB+ oocytes were in vitro maturated (40h) and involved to ICSI process. The presumptive embryos were in vitro cultured (15h). Raw boar semen and SpermCatch® as slowing medium were used. No differences were observed between periods regarding early embryonic development and maturation competence...
September 26, 2017: Polish Journal of Veterinary Sciences
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