journal
MENU ▼
Read by QxMD icon Read
search

Expert Review of Molecular Diagnostics

journal
https://www.readbyqxmd.com/read/28135893/recent-advances-in-biochemical-and-molecular-diagnostics-for-the-rapid-detection-of-antibiotic-resistant-enterobacteriaceae-a-focus-on-%C3%A3-lactam-resistance
#1
Jean-Winoc Decousser, Laurent Poirel, Patrice Nordmann
Drug resistance among bacteria is a scourge to patients and infectious disease and infection control specialists. The rapid detection of resistance is a challenge for clinical microbiologists who wish to prevent deleterious individual and collective consequences such as (i) delaying efficient antibiotic therapy, which worsens the survival rate of the most severely ill patients, or (ii) delaying the isolation of the carriers of multidrug-resistant bacteria and promoting outbreaks; this last consequence is of special concern, and there are an increasing number of approaches and market-based solutions in response...
January 30, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28103450/paper-based-analytical-devices-for-clinical-diagnosis-recent-advances-in-the-fabrication-techniques-and-sensing-mechanisms
#2
Mazhar Sher, Rachel Zhuang, Utkan Demirci, Waseem Asghar
There is a significant interest in developing inexpensive portable biosensing platforms for various applications including disease diagnostics, environmental monitoring, food safety, and water testing at the point-of-care (POC) settings. Current diagnostic assays available in the developed world require sophisticated laboratory infrastructure and expensive reagents. Hence, they are not suitable for resource-constrained settings with limited financial resources, basic health infrastructure, and few trained technicians...
January 19, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28092481/the-effect-of-main-urine-inhibitors-on-the-activity-of-different-dna-polymerases-in-loop-mediated-isothermal-amplification
#3
Jekaterina Jevtuševskaja, Katrin Krõlov, Indrek Tulp, Ülo Langel
BACKGROUND: The use of rapid amplification methods to detect pathogens in biological samples is mainly limited by the amount of pathogens present in the sample and the presence of inhibiting substances. Inhibitors can affect the amplification efficiency by either binding to the polymerase, interacting with the DNA, or interacting with the polymerase during primer extension. Amplification is performed using DNA polymerase enzymes and even small changes in their activity can influence the sensitivity and robustness of molecular assays Methods: The main purpose of this research was to examine which compounds present in urine inhibit polymerases with strand displacement activity...
January 16, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28058850/molecular-diagnostics-in-the-management-of-rhabdomyosarcoma
#4
Michael A Arnold, Fredric G Barr
A classification of rhabdomyosarcoma (RMS) with prognostic relevance has primarily relied on clinical features and histologic classification as either embryonal or alveolar RMS. The PAX3-FOXO1 and PAX7-FOXO1 gene fusions occur in 80% of cases with the alveolar subtype and are more predictive of outcome than histologic classification. Identifying additional molecular hallmarks that further subclassify RMS is an active area of research. Areas Covered: The authors review the current state of the PAX3-FOXO1 and PAX7-FOXO1 fusions as prognostic biomarkers...
January 6, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28043179/the-cobas%C3%A2-6800-8800-system-a-new-era-of-automation-in-molecular-diagnostics
#5
Bryan Cobb, Christian O Simon, Susan L Stramer, Barbara Body, P Shawn Mitchell, Natasa Reisch, Wendy Stevens, Sergio Carmona, Louis Katz, Stephen Will, Oliver Liesenfeld
Molecular diagnostics is a key component of laboratory medicine. Here, the authors review key triggers of ever-increasing automation in nucleic acid amplification testing (NAAT) with a focus on specific automated Polymerase Chain Reaction (PCR) testing and platforms such as the recently launched cobas® 6800 and cobas® 8800 Systems. The benefits of such automation for different stakeholders including patients, clinicians, laboratory personnel, hospital administrators, payers, and manufacturers are described...
January 3, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28004592/non-culture-based-diagnostics-for-intravascular-catheter-related-bloodstream-infections
#6
Li Zhang, C M Rickard
intravascular catheter related bloodstream infection (IVC-BSI) is a leading cause of nosocomial infections and associated with significant morbidity and mortality. Early detection and adequate treatment of causative pathogens is critical for a favourable outcome. However, it takes significant time to receive microbiological results due to the current reference diagnostic method's reliance on microbial growth. Areas covered: This review discusses culture and non-culture based techniques for the diagnosis of non IVC-BSI and IVC-BSI, including molecular methods and biomarkers...
January 2, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28034324/p16-overexpression-in-braf-mutated-gastrointestinal-stromal-tumors
#7
Shan-Shan Shi, Xuan Wang, Qiu-Yuan Xia, Qiu Rao, Qin Shen, Sheng-Bin Ye, Rui Li, Qun-Li Shi, Zhen-Feng Lu, Heng-Hui Ma, Xiao-Jun Zhou
BACKGROUND: The aims of this study were to analyze the histopathology, immunophenotype, molecular features, and prognosis in cases of BRAF-mutated gastrointestinal stromal tumors (GISTs) and to examine the p16 expression in these tumors, and further discuss its effects on tumor formation and progression. METHODS: In all, 283 GIST cases (201 KIT mutants, 12 PDGFRA mutants and 70 wild-type) from the 2010 to 2014 surgical pathology files of the Department of Pathology at Nanjing Jinling Hospital were analyzed for mutations in BRAF exon 15...
December 30, 2016: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/27900885/selecting-heart-failure-patients-for-metabolic-interventions
#8
Harmen G Booij, Anne M Koning, Harry van Goor, Rudolf A de Boer, B Daan Westenbrink
Heart failure (HF) has become the cardiovascular epidemic of the century and now imposes an immense burden on health care systems. While our understanding of the pathophysiology of HF has increased dramatically, the translation of knowledge into clinical practice has been disappointing. Metabolic dysfunction in HF has been studied for eight decades, but these efforts have not resulted in effective therapies. This paucity in clinical translation probably results from the variable contribution of metabolic dysfunction to the underlying heart disease...
December 28, 2016: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/27936985/t-lymphocytes-to-predict-radiation-induced-late-effects-in-normal-tissues
#9
Muriel Brengues, Ariane Lapierre, Céline Bourgier, André Pèlegrin, Mahmut Özsahin, David Azria
Radiotherapy is one of the main treatments for solid tumors. The total dose that can be delivered to the tumor is limited by the radiation amount received by the surrounding normal tissues, which are at risk of developing acute and late radiation-induced effects. Areas covered: Severe late radiation-induced toxicity occurs in 5% to 10% of patients following radiotherapy. However, the current radiotherapy and radiation protection protocols do not take into account the variations in radiosensitivity among individuals...
December 22, 2016: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/27936983/molecular-diagnosis-of-invasive-mycoses-of-the-central-nervous-system
#10
Matthew William McCarthy, Thomas J Walsh
In September 2012, the Centers for Disease Control and Prevention (CDC) began investigating an outbreak of fungal meningitis among patients who had received contaminated preservative-free methyl prednisolone acetate injections from the New England Compounding Center in Framingham, Massachusetts. Thousands of patients were potentially exposed to tainted corticosteroids, but establishing the diagnosis of fungal meningitis during the nationwide outbreak was difficult because little was known about the natural history of the disease...
December 22, 2016: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/27935358/convergent-lines-of-evidence-support-the-role-of-uric-acid-levels-as-a-potential-biomarker-in-bipolar-disorder
#11
Rodrigo Machado-Vieira, Haitham Salem, Benicio N Frey, Izabela G Barbosa, Antonio L Teixeira
No abstract text is available yet for this article.
December 20, 2016: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28218019/nucleic-acid-amplification-methodologies-for-the-detection-of-pulmonary-mold-infections
#12
Matthew William McCarthy, Ruta Petraitiene, Thomas J Walsh
The detection of pulmonary mold infections has historically required technically demanding methods obtained through invasive procedures. Nucleic acid amplification assays have the potential to circumvent the technical hurdles associated with diagnosis, but are not without potential pitfalls. Areas covered: In this paper, the authors review new assays for the diagnosis of pulmonary mold infections due to aspergillosis, mucormycosis, and hyalohyphomycoses as well as uncommon infections caused by dematiaceous molds...
March 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28218018/corrigendum
#13
(no author information available yet)
No abstract text is available yet for this article.
March 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28129709/profile-of-the-roche-cobas%C3%A2-egfr-mutation-test-v2-for-non-small-cell-lung-cancer
#14
Umberto Malapelle, Rafael Sirera, Eloísa Jantus-Lewintre, Pablo Reclusa, Silvia Calabuig-Fariñas, Ana Blasco, Pasquale Pisapia, Christian Rolfo, Carlos Camps
The discovery of driver mutations in non-small cell lung cancer (NSCLC) has led to the development of genome-based personalized medicine. Fifteen to 20% of adenocarcinomas harbor an epidermal growth factor receptor (EGFR) activating mutation associated with responses to EGFR tyrosine kinase inhibitors (TKIs). Individual laboratories' expertise and the availability of appropriate equipment are valuable assets in predictive molecular pathology, although the choice of methods should be determined by the nature of the samples to be tested and whether the detection of only well-characterized EGFR mutations or rather, of all detectable mutations, is required...
March 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28127999/newborn-screening-for-cystic-fibrosis-can-one-algorithm-fit-all
#15
Michele Caggana
No abstract text is available yet for this article.
March 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28118758/molecular-classification-of-gastric-cancer
#16
Christoph Röcken
Gastric cancer is among the most common cancers worldwide. Despite declining incidences, the prognosis remains dismal in Western countries and is better in Asian countries with national cancer screening programs. Complete endoscopic or surgical resection of the primary tumor with or without lymphadenectomy offers the only chance of cure in the early stage of the disease. Survival of more locally advanced gastric cancers was improved by the introduction of perioperative, adjuvant and palliative chemotherapy...
March 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28117603/he4-test-of-urine-and-body-fluids-for-diagnosis-of-gynecologic-cancer
#17
Wanglei Qu, Quansheng Gao, Haibin Chen, Zuoqing Tang, Xueqiong Zhu, Shi-Wen Jiang
Serum epididymis protein 4 (HE4) level is a useful biomarker for the management of ovarian and endometrial cancer patients. Urine HE4-test, with its easier access than serum test, has emerged as a new method with promising application for the diagnosis of ovarian cancer. Areas covered: This review summarizes data regarding the detection and alteration of HE4 in urine samples collected from ovarian cancer patients and controls. The performance and limitation of the assay and potential direction of future study are also discussed...
March 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28110577/preconception-carrier-screening-and-prenatal-diagnosis-in-thalassemia-and-hemoglobinopathies-challenges-and-future-perspectives
#18
Joanne Traeger-Synodinos, Cornelis L Harteveld
Hemoglobinopathies constitute the most common severe monogenic disorders worldwide, with an increasing global burden each year. The benefit of applying programmes for preconception carrier screening, with the option of prenatal diagnosis, to minimize the incidence of new cases is recognized in many countries. Areas covered: The challenges associated with identifying carrier couples using hematology-based screening, along with DNA diagnosis and prenatal diagnosis were addressed, based on a literature search and the authors expertise...
March 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28093921/microbial-genomics-and-antimicrobial-susceptibility-testing
#19
W Michael Dunne, Magali Jaillard, Olivier Rochas, Alex Van Belkum
Antimicrobial susceptibility testing is key in modern clinical microbiology. With pandemic emergence of (multi-)antibiotic resistance, methods to detect and quantify resistance of clinically important bacterial species are imperative. Historically, antimicrobial susceptibility testing (AST) was mostly performed using methods relying on bacterial growth. Such methods may be time-consuming and more rapid alternatives have been actively sought for. Areas covered: Among the new AST methods there are many that focus on detection of causal resistance genes and/or gene mutations...
March 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28092472/using-biomarkers-to-predict-and-to-prevent-cardiotoxicity-of-cancer-therapy
#20
Daniela Cardinale, Gina Biasillo, Michela Salvatici, Maria Teresa Sandri, Carlo Maria Cipolla
Cardiotoxicity is a common complication that may compromise the clinical effectiveness of anticancer therapy. The current standard for monitoring cardiac function detects cardiotoxicity only when a functional impairment has already occurred, not allowing for any early preventive strategy. Areas covered: A novel approach, based on the use of biomarkers has recently emerged, resulting in a very effective tool for early, real-time identification, and monitoring of cardiotoxicity. In particular, cardiac troponin elevation during chemotherapy allows to identify patients more prone to develop myocardial dysfunction and cardiac events...
March 2017: Expert Review of Molecular Diagnostics
journal
journal
39742
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"