Rui Gao, Guang Yang, Mengting Wang, Jing Xiao, Shanru Yi, Yanxin Huang, Zhenxiang Guo, Yunzhe Kang, Qianzheng Fu, Mingzhu Wang, Ben Xu, Shijun Shen, Qianshu Zhu, Meng Liu, Liping Wang, Xinyu Cui, Shanshan Yi, Xiaochen Kou, Yanhong Zhao, Liang Gu, Hong Wang, Shaorong Gao, Cizhong Jiang, Jiayu Chen
Transcription factors (TFs) play important roles in early embryonic development, but factors regulating TF action, relationships in signaling cascade, genome-wide localizations, and impacts on cell fate transitions during this process have not been clearly elucidated. In this study, we used uliCUT&RUN-seq to delineate a TFAP2C-centered regulatory network, showing that it involves promoter-enhancer interactions and regulates TEAD4 and KLF5 function to mediate cell polarization. Notably, we found that maternal retinoic acid metabolism regulates TFAP2C expression and function by inducing the active demethylation of SINEs, indicating that the RARG-TFAP2C-TEAD4/KLF5 axis connects the maternal-to-zygotic transition to polarization...
April 1, 2024: Developmental Cell