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Cell Communication & Adhesion

Florian B Stierlin, Filippo Molica, Jean-Luc Reny, Brenda R Kwak, Pierre Fontana
Pannexin1 (Panx1), a membrane channel-forming protein permitting the passage of small-sized molecules, such as ATP, is expressed in human platelets. Recently, we showed that inhibiting Panx1 affects collagen-induced platelet aggregation but not aggregation triggered by other agonists. We also found that a single nucleotide polymorphism (SNP; rs1138800) in the Panx1 gene encoded for a gain-of-function channel (Panx1-400C) and was associated with enhanced collagen-induced platelet reactivity. Here, we assessed the association of this SNP with platelet reactivity in a cohort of 758 stable cardiovascular patients from the ADRIE study treated with aspirin and/or clopidogrel...
December 2017: Cell Communication & Adhesion
Chuan Yu, Ming Li, Yanan Sun, Xingguo Wang, Yong Chen
As the initiation step of bacterial infection or biofouling, bacterial adhesion on cells or substrates is generally an optimal target for antibacterial design. Phosphatidylethanolamine (PE) is the principal phospholipid in bacteria, and its function in bacterial adhesion remains unclear. In this study, four E. coli strains including two PE-deficient mutants (PE(-)PC(-) and PE(-)PC(+ )strains) and two PE-containing wild-type controls (PE (+) PC(-) strains) were recruited to investigate the influence of PE deficiency on bacterial adhesion...
December 2017: Cell Communication & Adhesion
Sharareh Jalali, Mohammad Tafazzoli-Shadpour, Nooshin Haghighipour, Ramin Omidvar, Farzaneh Safshekan
Although substrate stiffness has been previously reported to affect various cellular aspects, such as morphology, migration, viability, growth, and cytoskeletal structure, its influence on cell adherence has not been well examined. Here, we prepared three soft, medium, and hard polyacrylamide (PAAM) substrates and utilized AFM to study substrate elasticity and also the adhesion and mechanical properties of endothelial cells in response to changing substrate stiffness. Maximum detachment force and cell stiffness were increased with increasing substrate stiffness...
April 2015: Cell Communication & Adhesion
Dorothea Fischbacher, Marion Merle, Anja Liepert, Christine Grabrucker, Tanja Kroell, Andreas Kremser, Julia Dreyßig, Markus Freudenreich, Friedhelm Schuster, Arndt Borkhardt, Doris Kraemer, Claus-Henning Koehne, Hans-Jochem Kolb, Christoph Schmid, Helga Maria Schmetzer
To enlighten interactions between autologous, allogeneic or T-cells from patients after stem cell transplantation with leukaemia-derived-dendritic-cells containing dendritic cells or blast containing mononuclear cells (n = 21, respectively), we determined cytokine-concentrations (interleukin 2, 4, 6, 10, tumor-necrosis-factor-α, interferon-γ) in supernatants of mixed-lymphocyte-culture and in serum (n = 16) of 20 patients with acute myeloid leukaemia and three patients with myelodysplastic syndromes by cytometric-bead-assay...
April 2015: Cell Communication & Adhesion
Mi-Hyun Nam, Won-Rak Son, Young Sik Lee, Kwang-Won Lee
Advanced glycation end-products (AGEs) are involved in the development of vascular smooth muscle cell (VSMC) dysfunction and the progression of atherosclerosis. However, AGEs may indirectly affect VSMCs via AGEs-induced signal transduction between monocytes and human umbilical endothelial cells (HUVECs), rather than having a direct influence. This study was designed to elucidate the signaling pathway underlying AGEs-RAGE axis influence on VSMC dysfunction using a co-culture system with monocytes, HUVECs and VSMCs...
April 2015: Cell Communication & Adhesion
Joost Willebrords, Sara Crespo Yanguas, Michaël Maes, Elke Decrock, Nan Wang, Luc Leybaert, Tereza Cristina da Silva, Isabel Veloso Alves Pereira, Hartmut Jaeschke, Bruno Cogliati, Mathieu Vinken
Gap junctions are a specialized group of cell-to-cell junctions that mediate direct intercellular communication between cells. They arise from the interaction of two hemichannels of adjacent cells, which in turn are composed of six connexin proteins. In liver, gap junctions are predominantly found in hepatocytes and play critical roles in virtually all phases of the hepatic life cycle, including cell growth, differentiation, liver-specific functionality and cell death. Liver gap junctions are directed through a broad variety of mechanisms ranging from epigenetic control of connexin expression to post-translational regulation of gap junction activity...
April 2015: Cell Communication & Adhesion
Xiujuan Wang, Kun Liu, Bin Li, Yanning Li, Kaiwei Ye, Jinsheng Qi, Yu Wang
Activated macrophages contribute to endothelial dysfunction; however, it is unclear how peroxynitrite contributes to macrophage-mediated human cardiac microvascular endothelial cell (HCMEC) injury in hypoxia. In macrophage-HCMEC co-cultures subjected to hypoxia, there was an increase in hypoxia-inducible factor (HIF)-1α, HIF-2α, inducible nitric oxide synthase (iNOS), endothelin-converting enzyme (ECE)-1 and cyclooxygenase-2 (COX-2), and concomitant decrease in prostacyclin synthase (PGIS). This was mimicked by a peroxynitrite donor and attenuated by its decomposition catalyst...
April 2015: Cell Communication & Adhesion
Otmar Huber, Iver Petersen
Desmosomes represent adhesive, spot-like intercellular junctions that in association with intermediate filaments mechanically link neighboring cells and stabilize tissue architecture. In addition to this structural function, desmosomes also act as signaling platforms involved in the regulation of cell proliferation, differentiation, migration, morphogenesis, and apoptosis. Thus, deregulation of desmosomal proteins has to be considered to contribute to tumorigenesis. Proteolytic fragmentation and downregulation of desmosomal cadherins and plaque proteins by transcriptional or epigenetic mechanisms were observed in different cancer entities suggesting a tumor-suppressive role...
2015: Cell Communication & Adhesion
George Malietzis, Gui H Lee, John T Jenkins, David Bernardo, Morgan Moorghen, Stella C Knight, Hafid O Al-Hassi
Dendritic cells (DCs) either boost the immune system (enhancing immunity) or dampen it (leading to tolerance). This dual effect explains their vital role in cancer development and progression. DCs have been tested as a predictor of outcomes for cancer progression. Eight studies evaluated tumour-infiltrating DCs (TIDCs) as a predictor for colorectal cancer (CRC) outcomes. The detection of TIDCs has not kept pace with the increased knowledge about the identification of DC subsets and their maturation status. For that reason, it is difficult to draw a conclusion about the performance of DCs as a predictor of outcome for CRC...
2015: Cell Communication & Adhesion
Panagiotis Apostolou, Maria Toloudi, Marina Chatziioannou, Eleni Kourtidou, Georgia Mimikakou, Ioanna Vlachou, Aikaterini Chlichlia, Ioannis Papasotiriou
Epithelial-to-mesenchymal transition (EMT) as well as the reverse process, mesenchymal-to-epithelial transition (MET) is important during embryogenesis. EMT is also involved in cancer invasion and metastasis, and can generate cells with properties similar to those of stem cells. Retrotransposons can rearrange the genome by inserting DNA in new loci, thus inducing mutations. This study examines the gene expression of transcription factors involved in EMT and MET. In the second experimental panel, the gene expression of L1 retrotransposon was studied...
2015: Cell Communication & Adhesion
Yasuo Kitajima
Desmosomes are the most important intercellular adhering junctions that adhere two adjacent keratinocytes directly with desmosomal cadherins, that is, desmogleins (Dsgs) and desmocollins, forming an epidermal sheet. Recently, two cell-cell adhesion states of desmosomes, that is, "stable hyper-adhesion" and "dynamic weak-adhesion" conditions have been recognized. They are mutually reversible through cell signaling events involving protein kinase C (PKC), Src and epidermal growth factor receptor (EGFR) during Ca(2+)-switching and wound healing...
December 2014: Cell Communication & Adhesion
Sathappan Shanthi, Sivalingam Manju, Perumal Rajakumaran, Baskaralingam Vaseeharan
The cDNA sequence of peroxinectin was obtained from the haemocytes of Indian white shrimp Fenneropenaeus indicus using RT-PCR and RACE. Fenneropenaeus indicus peroxinectin (Fi-Pxn) sequence has an open reading frame (ORF) of 2415 bp encoding a protein of 804 amino acids with 21 residues signal sequence. The mature protein has molecular mass of 89.8 kDa with an estimated pI of 8.6. Two putative integrin-binding motifs, RGD and KGD, were observed at the basic N-terminal and C-terminal part of the mature aminoacid sequence...
December 2014: Cell Communication & Adhesion
Chandrabose Selvaraj, Ramanathan Bharathi Priya, Sanjeev Kumar Singh
Bacillus anthracis is a pathogenic, Gram-positive bacterium which chiefly affects the livestock of animals and humans through acute disease anthrax. All around the globe this bio-threat organism damages millions of lives in every year and also most of the drugs were not responding properly in inhibition against this diseased pathogen. In recent development, phage therapy is considered as alternative solution to treat this serious infectious disease. In this study, we elucidated the binding of γ phage lysin plyG enzymes toward the SrtA along with its activator peptide LPXTG...
October 2014: Cell Communication & Adhesion
David Garrod, Lydia Tabernero
Hyper-adhesion is a unique, strongly adhesive form of desmosomal adhesion that functions to maintain tissue integrity. In this short review, we define hyper-adhesion, summarise the evidence for it in culture and in vivo, discuss its role in development, wound healing, and skin disease, and speculate about its molecular and cellular basis.
October 2014: Cell Communication & Adhesion
Pooyan Sadr-Eshkevari, Sajjad Ashnagar, Ashkan Rashad, Marisa Dietz, Jochen Jackowski, Amr Abdulazim, Nora Prochnow
Bisphosphonates (BPs) are drugs commonly used in the treatment of various disease arising or affecting bone tissue. There is a standard use in bone neoplasia and metastasis, hormonal and developmental disorders as well as for compensation of adverse effects in several medical therapies. Many in-vivo and in-vitro studies have assessed the efficacy of this drug and its function in cellular scale. In this concern, BPs are described to inhibit the resorptive function of osteoclasts and to prevent apoptosis of osteoblasts and osteocytes...
October 2014: Cell Communication & Adhesion
Jeyachandran Sivakamavalli, Oyyappan Deepa, Baskaralingam Vaseeharan
Ruta graveolens silver nanoparticles (AgNPs) showed the color change within 30 min and characterized using UV-visible spectra, Fourier Transform Infrared (FTIR), X-ray Diffraction (XRD) and Transmission Electron Microscopy (TEM). UV-visible spectrum of R. graveolens AgNPs showed the sharp peak at the wavelength of 440-560 nm. XRD patterns confirmed that crystalline nature of R. graveolens AgNPs and FTIR results revealed that phytochemical reaction of these R. graveolens is responsible for the synthesis of AgNPs...
August 2014: Cell Communication & Adhesion
Bechara A Saykali, Mirvat El-Sibai
The occurrence of invadopodia has been, since its characterization, a hallmark of cancerous cell invasion and metastasis. These structures are now the subject of a controversy concerning their cellular function, molecular regulation, and assembly. The terms invadopodia and podosomes have been used interchangeably since their discovery back in 1980. Since then, these phenotypes are now more established and accepted by the scientific community as vital structures for 3D cancer cell motility. Many characteristics relating to invadopodia and podosomes have been elucidated, which might prove these structures as good targets for metastasis treatment...
August 2014: Cell Communication & Adhesion
Gerardo Arrevillaga-Boni, Marcela Hernández-Ruiz, Elena Cristina Castillo, Vianney Ortiz-Navarrete
Cell-to-cell information exchange mediated by membrane protrusions in tunneling nanotubes (TNTs) has been widely described in distinct cell lines. Here, we describe a new form of direct intercellular communication in a murine macrophage-like cell line that is mediated by pseudopodial fusions that form over scraped plastic tissue culture surfaces along scratch lines. These structures are capable of forming intercellular, tunnel-like channels (inter-pseudopodial axis connections) that can be differentiated from TNTs based on length, thickness, tandem arrangement along an axis, pseudopodial origin and permanency...
August 2014: Cell Communication & Adhesion
Vishwas Tripathi, Romsha Kumar, Amit K Dinda, Jagdeep Kaur, Kalpana Luthra
Abstract CXCL12 acts as a physiological ligand for the chemokine receptor CXCR7. Chemokine receptor expression by human trophoblast and other placental cells have important implications for understanding the regulation of placental growth and development. We had previously reported the differential expression of CXCR7 in different stages of the human placenta suggesting its possible role in regulation of placental growth and development. In this study, we determined the expression of CXCR7 in human choriocarcinoma JAR cells at the mRNA level and protein level and the downstream signaling pathway mediated by CXCL12-CXCR7 interaction...
August 2014: Cell Communication & Adhesion
Werner Franke, Pamela Cowin
No abstract text is available yet for this article.
June 2014: Cell Communication & Adhesion
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