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BMC Biotechnology

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https://www.readbyqxmd.com/read/28633643/enhancement-of-thermoalkaliphilic-xylanase-production-by-pichia-pastoris-through-novel-fed-batch-strategy-in-high-cell-density-fermentation
#1
Tingting Shang, Dayong Si, Dongyan Zhang, Xuhui Liu, Longmei Zhao, Cong Hu, Yu Fu, Rijun Zhang
BACKGROUND: Xylanase degrades xylan into monomers of various sizes by catalyzing the endohydrolysis of the 1,4-β-D-xylosidic linkage randomly, possessing potential in wide industrial applications. Most of xylanases are susceptible to be inactive when suffering high temperature and high alkaline process. Therefore, it is necessary to develop a high amount of effective thermoalkaliphilic xylanases. This study aims to enhance thermoalkaliphilic xylanase production in Pichia pastoris through fermentation parameters optimization and novel efficient fed-batch strategy in high cell-density fermentation...
June 21, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28637431/continuous-long-term-cytotoxicity-monitoring-in-3d-spheroids-of-beetle-luciferase-expressing-hepatocytes-by-nondestructive-bioluminescence-measurement
#2
Mayu Yasunaga, Yasuko Fujita, Rumiko Saito, Mitsuo Oshimura, Yoshihiro Nakajima
BACKGROUND: Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated treatment of the spheroids with a test object is possible. Generally, in the repeated treatment test to assess cytotoxicity in the spheroids, ATP assay, colorimetric measurement using pigments or high-content imaging analysis is performed. However, continuous assessment of cytotoxicity in the same spheroids using the above assays or analysis is impossible because the spheroids must be disrupted or killed...
June 20, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28629346/a-hyper-thermostable-%C3%AE-amylase-from-pyrococcus-furiosus-accumulates-in-nicotiana-tabacum-as-functional-aggregates
#3
Hong Zhu, L Bruce Reynolds, Rima Menassa
BACKGROUND: Alpha amylase hydrolyzes α-bonds of polysaccharides such as starch and produces malto-oligosaccharides. Its starch saccharification applications make it an essential enzyme in the textile, food and brewing industries. Commercially available α-amylase is mostly produced from Bacillus or Aspergillus. A hyper-thermostable and Ca (2++) independent α-amylase from Pyrococcus furiosus (PFA) expressed in E.coli forms insoluble inclusion bodies and thus is not feasible for industrial applications...
June 19, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28606082/expression-and-functional-analyses-of-a-kinesin-gene-ghkis13a1-from-cotton-gossypium-hirsutum-fiber
#4
Yan-Jun Li, Shou-Hong Zhu, Xin-Yu Zhang, Yong-Chang Liu, Fei Xue, Lan-Jie Zhao, Jie Sun
BACKGROUND: Cotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. A large number of genes are believed to be involved in fiber formation, but so far only a few fiber genes have been isolated and functionally characterized in this developmental process. The Kinesin13 subfamily was found to play key roles during cell division and cell elongation, and was considered to be involved in the regulation of cotton fiber development...
June 12, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28606077/evaluation-of-gaussia-luciferase-and-foot-and-mouth-disease-virus-2a-translational-interrupter-chimeras-as-polycistronic-reporters-for-transgene-expression
#5
Michael Puckette, Thomas Burrage, John G Neilan, Max Rasmussen
BACKGROUND: The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simultaneously quantitate production of other recombinant proteins by transcriptionally linking the Gaussia princeps luciferase gene to other genes of interest through the foot-and-mouth disease virus 2A translational interrupter sequence. RESULTS: We produced six plasmids, each encoding a single open reading frame, with the foot-and-mouth disease virus 2A sequence placed either N-terminal or C-terminal to the Gaussia princeps luciferase gene...
June 12, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28606076/flocculating-performance-of-a-bioflocculant-produced-by-arthrobacter-humicola-in-sewage-waste-water-treatment
#6
Mayowa Oladele Agunbiade, Esta Van Heerden, Carolina H Pohl, Anofi Tom Ashafa
BACKGROUND: The discharge of poorly treated effluents into the environment has far reaching, consequential impacts on human and aquatic life forms. Thus, we evaluated the flocculating efficiency of our test bioflocculant and we report for the first time the ability of the biopolymeric flocculant produced by Arthrobacter humicola in the treatment of sewage wastewater. This strain was isolated from sediment soil sample at Sterkfontein dam in the Eastern Free State province of South Africa...
June 12, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28587679/somatic-cell-selection-for-chlorsulfuron-resistant-mutants-in-potato-identification-of-point-mutations-in-the-acetohydroxyacid-synthase-gene
#7
Philippa J Barrell, Julie M Latimer, Samantha J Baldwin, Michelle L Thompson, Jeanne M E Jacobs, Anthony J Conner
BACKGROUND: Somatic cell selection in plants allows the recovery of spontaneous mutants from cell cultures. When coupled with the regeneration of plants it allows an effective approach for the recovery of novel traits in plants. This study undertook somatic cell selection in the potato (Solanum tuberosum L.) cultivar 'Iwa' using the sulfonylurea herbicide, chlorsulfuron, as a positive selection agent. RESULTS: Following 5 days' exposure of potato cell suspension cultures to 20 μg/l chlorsulfuron, rescue selection recovered rare potato cell colonies at a frequency of approximately one event in 2...
June 6, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28577554/fermentation-of-lactose-to-ethanol-in-cheese-whey-permeate-and-concentrated-permeate-by-engineered-escherichia-coli
#8
Lorenzo Pasotti, Susanna Zucca, Michela Casanova, Giuseppina Micoli, Maria Gabriella Cusella De Angelis, Paolo Magni
BACKGROUND: Whey permeate is a lactose-rich effluent remaining after protein extraction from milk-resulting cheese whey, an abundant dairy waste. The lactose to ethanol fermentation can complete whey valorization chain by decreasing dairy waste polluting potential, due to its nutritional load, and producing a biofuel from renewable source at the same time. Wild type and engineered microorganisms have been proposed as fermentation biocatalysts. However, they present different drawbacks (e...
June 2, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28558675/development-of-plant-produced-protein-body-vaccine-candidates-for-bluetongue-virus
#9
Albertha R van Zyl, Ann E Meyers, Edward P Rybicki
BACKGROUND: Bluetongue is a disease of domestic and wild ruminants caused by bluetongue virus serotypes (BTV), which have caused serious outbreaks worldwide. Commercially available vaccines are live-attenuated or inactivated virus strains: these are effective, but there is the risk of reversion to virulence or reassortment with circulating strains for live virus, and residual live virus for the inactivated vaccines. The live-attenuated virus vaccines are not able to distinguish naturally infected animals from vaccinated animals (DIVA compliant)...
May 30, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28545553/pigment-fingerprint-profile-during-extractive-fermentation-with-monascus-anka-gim-3-592
#10
Kan Shi, Rui Tang, Tao Huang, Lu Wang, Zhenqiang Wu
BACKGROUND: Traditional submerged fermentation mainly accumulates intracellular orange pigments with absorption maxima at 470 nm, whereas extractive fermentation of Monascus spp. with Triton X-100 can promote the export of intracellular pigments to extracellular broth, mainly obtaining extracellular yellow pigments with absorption maxima at approximately 410 nm. In this study, a strain of Monascus (M. anka GIM 3.592) that produces high yields of pigments was employed to investigate the differences in pigment fingerprint profiles between submerged and extractive fermentations...
May 25, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28521816/biochemical-characterization-of-the-xylan-hydrolysis-profile-of-the-extracellular-endo-xylanase-from-geobacillus-thermodenitrificans-t12
#11
Martinus J A Daas, Patricia Murciano Martínez, Antonius H P van de Weijer, John van der Oost, Willem M de Vos, Mirjam A Kabel, Richard van Kranenburg
BACKGROUND: Endo-xylanases are essential in degrading hemicellulose of various lignocellulosic substrates. Hemicellulose degradation by Geobacillus spp. is facilitated by the hemicellulose utilization (HUS) locus that is present in most strains belonging to this genus. As part of the HUS locus, the xynA gene encoding an extracellular endo-xylanase is one of the few secreted enzymes and considered to be the key enzyme to initiate hemicellulose degradation. Several Geobacillus endo-xylanases have been characterized for their optimum temperature, optimum pH and generation of degradation products...
May 18, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28521780/ultrasound-and-microbubble-induced-release-from-intracellular-compartments
#12
Farah Hussein, Costin Antonescu, Raffi Karshafian
BACKGROUND: Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB-induced release of cellular materials to the extracellular milieu. This study investigates USMB effects on the molecular release from cells through membrane-disruption and exocytosis...
May 18, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28506256/aav-vector-distribution-in-the-mouse-respiratory-tract-following-four-different-methods-of-administration
#13
Lisa A Santry, Joelle C Ingrao, Darrick L Yu, Jondavid G de Jong, Laura P van Lieshout, Geoffrey A Wood, Sarah K Wootton
BACKGROUND: Targeted delivery of gene therapy vectors to the mouse respiratory tract is often performed via intranasal or intratracheal administration; however, there can be a great deal of variability between these methods, which could potentially influence experimental results. Improving the accuracy and precision of lung delivery will not only reduce the number of animals required to detect statistically significant differences, but may reduce the variability of studies from different laboratories...
May 15, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28499450/intrabodies-against-the-polysialyltransferases-st8siaii-and-st8siaiv-inhibit-polysialylation-of-ncam-in-rhabdomyosarcoma-tumor-cells
#14
Stefan Somplatzki, Martina Mühlenhoff, Andrea Kröger, Rita Gerardy-Schahn, Thomas Böldicke
BACKGROUND: Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8SiaII and ST8SiaIV. Intracellular antibodies (intrabodies; IB) expressed inside the ER and retaining proteins passing the ER such as cell surface receptors or secretory proteins provide an efficient means of protein knockdown...
May 12, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28482870/effect-of-the-cancer-specific-shorter-form-of-human-6-phosphofructo-1-kinase-on-the-metabolism-of-the-yeast-saccharomyces-cerevisiae
#15
Darjan Andrejc, Alenka Možir, Matic Legiša
BACKGROUND: At first glance, there appears to be a high degree of similarity between the metabolism of yeast (the Crabtree effect) and human cancer cells (the Warburg effect). At the root of both effects is accelerated metabolic flow through glycolysis which leads to overflows of ethanol and lactic acid, respectively. It has been proposed that enhanced glycolytic flow in cancer cells is triggered by the altered kinetic characteristics of the key glycolytic regulatory enzyme 6-phosphofructo-1-kinase (Pfk)...
May 8, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28464851/pollen-mediated-gene-flow-from-transgenic-to-non-transgenic-switchgrass-panicum-virgatum-l-in-the-field
#16
Reginald Millwood, Madhugiri Nageswara-Rao, Rongjian Ye, Ellie Terry-Emert, Chelsea R Johnson, Micaha Hanson, Jason N Burris, Charles Kwit, C Neal Stewart
BACKGROUND: Switchgrass is C4 perennial grass species that is being developed as a cellulosic bioenergy feedstock. It is wind-pollinated and considered to be an obligate outcrosser. Genetic engineering has been used to alter cell walls for more facile bioprocessing and biofuel yield. Gene flow from transgenic cultivars would likely be of regulatory concern. In this study we investigated pollen-mediated gene flow from transgenic to nontransgenic switchgrass in a 3-year field experiment performed in Oliver Springs, Tennessee, U...
May 2, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28449656/improved-tissue-culture-conditions-for-the-emerging-c4-model-panicum-hallii
#17
Joshua N Grant, Jason N Burris, C Neal Stewart, Scott C Lenaghan
BACKGROUND: Panicum hallii Vasey (Hall's panicgrass) is a compact, perennial C4 grass in the family Poaceae, which has potential to enable bioenergy research for switchgrass (Panicum virgatum L.). Unlike P. hallii, switchgrass has a large genome, allopolyploidy, self-incompatibility, a long life cycle, and large stature-all suboptimal traits for rapid genetics research. Herein we improved tissue culture methodologies for two inbred P. hallii populations: FIL2 and HAL2, to enable further development of P...
April 27, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28407739/construction-of-a-novel-anaerobic-pathway-in-escherichia-coli-for-propionate-production
#18
Jing Li, Xinna Zhu, Jing Chen, Dongdong Zhao, Xueli Zhang, Changhao Bi
BACKGROUND: Propionate is widely used as an important preservative and important chemical intermediate for synthesis of cellulose fibers, herbicides, perfumes and pharmaceuticals. Biosynthetic propionate has mainly been produced by Propionibacterium, which has various limitations for industrial application. RESULTS: In this study, we engineered E. coli by combining reduced TCA cycle with the native sleeping beauty mutase (Sbm) cycle to construct a redox balanced and energy viable fermentation pathway for anaerobic propionate production...
April 14, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28399854/improved-n-butanol-production-via-co-expression-of-membrane-targeted-tilapia-metallothionein-and-the-clostridial-metabolic-pathway-in-escherichia-coli
#19
Wei-Chih Chin, Kuo-Hsing Lin, Chun-Chi Liu, Kenji Tsuge, Chieh-Chen Huang
BACKGROUND: N-Butanol has favorable characteristics for use as either an alternative fuel or platform chemical. Bio-based n-butanol production using microbes is an emerging technology that requires further development. Although bio-industrial microbes such as Escherichia coli have been engineered to produce n-butanol, reactive oxygen species (ROS)-mediated toxicity may limit productivity. Previously, we show that outer-membrane-targeted tilapia metallothionein (OmpC-TMT) is more effective as an ROS scavenger than human and mouse metallothioneins to reduce oxidative stress in the host cell...
April 11, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28399848/distinctive-molecular-and-biochemical-characteristics-of-a-glycoside-hydrolase-family-20-%C3%AE-n-acetylglucosaminidase-and-salt-tolerance
#20
Junpei Zhou, Zhifeng Song, Rui Zhang, Rui Liu, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han, Zunxi Huang
BACKGROUND: Enzymatic degradation of chitin has attracted substantial attention because chitin is an abundant renewable natural resource, second only to lignocellulose, and because of the promising applications of N-acetylglucosamine in the bioethanol, food and pharmaceutical industries. However, the low activity and poor tolerance to salts and N-acetylglucosamine of most reported β-N-acetylglucosaminidases limit their applications. Mining for novel enzymes from new microorganisms is one way to address this problem...
April 11, 2017: BMC Biotechnology
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