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BMC Biotechnology

Lei Wang, Sheng Chen, Jing Wu
BACKGROUND: Cyclodextrin glycosyltransferases (CGTases) catalyze the synthesis of cyclodextrins, which are circular α-(1,4)-linked glucans used in many applications in the industries related to food, pharmaceuticals, cosmetics, chemicals, and agriculture, among others. Economic use of these CGTases, particularly γ-CGTase, requires their efficient production. In this study, the effects of chemical chaperones, temperature and inducers on cell growth and the production of soluble γ-CGTase by Escherichia coli were investigated...
November 12, 2018: BMC Biotechnology
Hesham A El Enshasy, Elsayed Ahmed Elsayed, Noorhamizah Suhaimi, Roslinda Abd Malek, Mona Esawy
BACKGROUND: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4 billion by 2020. RESULTS: The production medium was first optimized using a statistical optimization approach to increase pectinase production. A maximal enzyme concentration of 76.35 U/mL (a 2.8-fold increase compared with the initial medium) was produced in a medium composed of (g/L): pectin, 32...
November 9, 2018: BMC Biotechnology
Santhanasabapathy Rajasekaran, Jayashree Thatte, Jayaprakash Periasamy, Alok Javali, Manjunath Jayaram, Dwaipayan Sen, Akshaya Krishnagopal, Giridhara R Jayandharan, Ramkumar Sambasivan
BACKGROUND: Recombinant adeno-associated viruses (AAVs) are emerging as favoured transgene delivery vectors for both research applications and gene therapy. In this context, a thorough investigation of the potential of various AAV serotypes to transduce specific cell types is valuable. Here, we rigorously tested the infectivity of a number of AAV serotypes in murine testis by direct testicular injection. RESULTS: We report the tropism of serotypes AAV2, 5, 8, 9 and AAVrh10 in mouse testis...
November 1, 2018: BMC Biotechnology
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No abstract text is available yet for this article.
October 29, 2018: BMC Biotechnology
Bao Tang, Cheng Sun, Yancun Zhao, Huiyong Xu, Gaoge Xu, Fengquan Liu
BACKGROUND: Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has reported on the fermentation process involved, representing huge obstacles for large-scale industrial production. RESULTS: Medium capacity, culture temperature, and fermentation time were identified as the most significant factors affecting the production of HSAF and employed for further optimization through statistical methods...
October 24, 2018: BMC Biotechnology
Dmitry Miroshnichenko, Danila Ashin, Alexander Pushin, Sergey Dolgov
BACKGROUND: Domesticated einkorn (Triticum monococcum L.) is one of the oldest cultivated cereal crops in the world. Its small genome size (~ 5.7 GB), low ploidy (2n = 2x = 14, Am Am ) and high genetic polymorphism make this species very attractive for use as a diploid model for understanding the genomics and proteomics of Triticeae. Einkorn, however, is still a recalcitrant monocotyledonous species for the application of modern biotechnologies, including transgenesis. This paper reports the factors that may influence transgene delivery, integration, expression and inheritance in einkorn...
October 23, 2018: BMC Biotechnology
Zhaojun Zheng, Xubiao Wei, Tingting Shang, Yan Huang, Cong Hu, Rijun Zhang
BACKGROUND: As the protein-laden by-product, red blood cells (RBCs) from poultry blood is a potential source of protein used as food and feed ingredient. However, RBC was currently underutilized. Therefore, it is an urgent need to develop feasible and cost-effective methods for converting poultry waste into nutritional and functional products. RESULTS: To take full advantage of this poultry waste, peptide hydrolysate was produced by deep controllable bioconversion of RBC, by means of synergistic combination of neutrase and flavourzyme...
October 20, 2018: BMC Biotechnology
Xiaofeng Liu, Min Wang, Yufeng Qin, Xuan Shi, Peiqing Cong, Yaosheng Chen, Zuyong He
BACKGROUND: Targeted DNA integration is widely used in basic research and commercial applications because it eliminates positional effects on transgene expression. Targeted integration in mammalian cells is generally achieved through a double crossover event between the genome and a linear donor containing two homology arms flanking the gene of interest. However, this strategy is generally less efficient at introducing larger DNA fragments. Using the homology-independent NHEJ mechanism has recently been shown to improve efficiency of integrating larger DNA fragments at targeted sites, but integration through this mechanism is direction-independent...
October 19, 2018: BMC Biotechnology
Azat Akhmetov, Andrew D Ellington, Edward M Marcotte
BACKGROUND: Encoding arbitrary digital information in DNA has attracted attention as a potential avenue for large scale and long term data storage. However, in order to enable DNA data storage technologies there needs to be improvements in data storage fidelity (tolerance to mutation), the facility of writing and reading the data (biases and systematic error arising from synthesis and sequencing), and overall scalability. RESULTS: To this end, we have developed and implemented an encoding scheme that is suitable for detecting and correcting errors that may arise during storage, writing, and reading, such as those arising from nucleotide substitutions, insertions, and deletions...
October 17, 2018: BMC Biotechnology
Anhar Danial Mustafa, Jeevanathan Kalyanasundram, Sarah Sabidi, Adelene Ai-Lian Song, Maha Abdullah, Raha Abdul Rahim, Khatijah Yusoff
BACKGROUND: Tuberculosis is one of the most common and deadliest infectious diseases worldwide affecting almost a third of the world's population. Although this disease is being prevented and controlled by the Bacille Calmette Guérin (BCG) vaccine, the protective efficacy is highly variable and substandard (0-80%) in adults. Therefore, novel and effective tuberculosis vaccine that can overcome the limitations from BCG vaccine need to be developed. RESULTS: A novel approach of utilizing an in-trans protein surface display system of Lactobacillus plantarum carrying and displaying combination of Mycobacterium tuberculosis subunit epitope antigens (Ag85B, CFP-10, ESAT-6, Rv0475 and Rv2031c) fused with LysM anchor motif designated as ACERL was constructed, cloned and expressed in Esherichia coli Rossetta expression host...
October 11, 2018: BMC Biotechnology
Meng Wang, Kaiwen Zheng, Jinglian Lin, Minhua Huang, Yi Ma, Shan Li, Xiaochun Luo, Jufang Wang
BACKGROUND: Cecropin A (CeA), a natural cationic antimicrobial peptide, exerts potent antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria, making it an attractive candidate substitute for antimicrobials. However, the low production rate and cumbersome, expensive processes required for both its recombinant and chemical synthesis have seriously hindered the exploitation and application of CeA. Here, we utilized a short β-structured self-aggregating protein, ELK16, as a fusion partner of CeA, which allowed the efficient production of high-purity CeA antibacterial peptide with a simple inexpensive process...
October 5, 2018: BMC Biotechnology
Ji-Xin Tang, Dafeng Chen, Shou-Long Deng, Jian Li, Yuanyuan Li, Zheng Fu, Xiu-Xia Wang, Yan Zhang, Su-Ren Chen, Yi-Xun Liu
BACKGROUND: Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) has been wildly used to generate gene knockout models through inducing indels causing frame-shift. However, there are few studies concerning the post-transcript effects caused by CRISPR-mediated genome editing. RESULTS: In the present study, we showed that gene knockdown model also could be generated using CRISPR-mediated gene editing by disrupting the boundary of exon and intron in mice (C57BL/6 J)...
October 3, 2018: BMC Biotechnology
Dafeng Chen, Ji-Xin Tang, Beibei Li, Li Hou, Xianhui Wang, Le Kang
BACKGROUND: The CRISPR/Cas9 system has been widely used to generate gene knockout/knockin models by inducing frameshift mutants in cell lines and organisms. Several recent studies have reported that such mutants can lead to in-frame exon skipping in cell lines. However, there was little research about post-transcriptional effect of CRISPR-mediated gene editing in vivo. RESULTS: We showed that frameshift indels also induced complete or stochastic exon skipping by deleting different regions to influence pre-mRNA splicing in vivo...
September 25, 2018: BMC Biotechnology
Yi Li, Yanting Xu, Ruixue Song, Congqi Tian, Lei Liu, Tianling Zheng, Hailei Wang
BACKGROUND: Microbial flocculation is a good choice for harvest of microalgae biomass, which has gained extensive attention. There have been carried out massive studies in bacterial flocculation, many bacterial strains with flocculation activity were isolated and different types of bioflocculants were produced. However, harvest of algal biomass by bioflocculants which produced from actinomycete are deficiency. In this study, the bioflocculant from an actinomycete Streptomyces sp. hsn06 could be used to harvest Chlorella vulgaris biomass...
September 21, 2018: BMC Biotechnology
Pingping Jiang, Ke Zhang, Zhaohua Ding, Qiuxia He, Wendi Li, Shuangfeng Zhu, Wen Cheng, Kewei Zhang, Kunpeng Li
BACKGROUND: Transgenic technology has become an important technique for crop genetic improvement. The application of well-characterized promoters is essential for developing a vector system for efficient genetic transformation. Therefore, isolation and functional validation of more alternative constitutive promoters to the CaMV35S promoter is highly desirable. RESULTS: In this study, a 2093-bp sequence upstream of the translation initiation codon ATG of AtSCPL30 was isolated as the full-length promoter (PD1)...
September 21, 2018: BMC Biotechnology
Hui Qian, Chong Zhang, Zhaoxin Lu, Bingjie Xia, Xiaomei Bie, Haizhen Zhao, Fengxia Lu, Guang-Yu Yang
BACKGROUND: Lipoxygenase (LOX) from Anabaena sp. PCC 7120 (Ana-rLOX) offers important applications in the food industry, especially for improving aroma and dough rheological properties. However, industrial applications of LOXs have been limited by their poor thermostability. Herein, we report a bioinformatics-based consensus concept approach for the engineering of thermostable Ana-rLOX. RESULTS: A series of mutations (N130D, G260A, S437T, N130D/G260Q, N130D/S437Y) showed higher thermostability and activity than the wild-type enzyme...
September 20, 2018: BMC Biotechnology
Hirotatsu Ohkawara, Shigeru Miyagawa, Satsuki Fukushima, Shin Yajima, Atsuhiro Saito, Hiroshi Nagashima, Yoshiki Sawa
BACKGROUND: Tissue-engineered cardiac constructs have potential in the functional recovery of heart failure; however, the preservation of these constructs is crucial for the development and widespread application of this treatment. We hypothesized that tissue-engineered skeletal myoblast (SMB) constructs may be preserved by vitrification to conserve biological function and structure. RESULTS: Scaffold-free cardiac cell-sheet constructs were prepared from SMBs and immersed in a vitrification solution containing ethylene glycol, sucrose, and carboxyl poly-L-lysine...
September 10, 2018: BMC Biotechnology
Md Kausar Alam, Michelle Brabant, Raja Solomon Viswas, Kris Barreto, Humphrey Fonge, C Ronald Geyer
BACKGROUND: Advances in antibody engineering provide strategies to construct recombinant antibody-like molecules with modified pharmacokinetic properties. Multermerization is one strategy that has been used to produce antibody-like molecules with two or more antigen binding sites. Multimerization enhances the functional affinity (avidity) and can be used to optimize size and pharmacokinetic properties. Most multimerization strategies involve genetically fusing or non-covalently linking antibody fragments using oligomerization domains...
September 10, 2018: BMC Biotechnology
Guotian Li, Kyle C Jones, Aymerick Eudes, Venkataramana R Pidatala, Jian Sun, Feng Xu, Chengcheng Zhang, Tong Wei, Rashmi Jain, Devon Birdseye, Patrick E Canlas, Edward E K Baidoo, Phat Q Duong, Manoj K Sharma, Seema Singh, Deling Ruan, Jay D Keasling, Jenny C Mortimer, Dominique Loqué, Laura E Bartley, Henrik V Scheller, Pamela C Ronald
BACKGROUND: Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression of OsAT10, a BAHD acyltransferase gene, enhances saccharification efficiency in rice. RESULTS: Here we show that overexpression of the rice OsAT10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T0 plants under greenhouse conditions...
September 4, 2018: BMC Biotechnology
Jian Xu, Yongling Zhu
BACKGROUND: The LoxP site based genetic switch, the FLEX, also known as DIO (Double-Floxed Inverted Open reading frame), was invented to turn on gene expression via Cre-mediated recombination. Since its first publication, numerous FLEX switch plasmids have been generated. These plasmids are designed to only work in combination with Cre. However on many occasions it is necessary to covert these FLEX plasmids back into constitutive expression plasmids so that they can also be used in non-Cre-expressing cells and in non-genetically modified animal models...
August 31, 2018: BMC Biotechnology
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