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Bioprocess and Biosystems Engineering

Weishuai Lian, Weifei Wang, Chin Ping Tan, Jianrong Wang, Yonghua Wang
LML-type structured lipids are one type of medium- and long-chain triacylglycerols. LML was synthesized using immobilized Talaromyces thermophilus lipase (TTL)-catalyzed interesterification of tricaprylin and ethyl linoleate. The resin AB-8 was chosen, and the lipase/support ratio was determined to be 60 mg/g. Subsequently, the immobilized TTL with strict sn-1,3 regiospecificity was applied to synthesize LML. Under the optimized conditions (60 °C, reaction time 6 h, enzyme loading of 6% of the total weight of substrates, substrate of molar ratio of ethyl linoleate to tricaprylin of 6:1), Triacylglycerols with two long- and one medium-chain FAs (DL-TAG) content as high as 52...
November 12, 2018: Bioprocess and Biosystems Engineering
Remya Vijayan, Siby Joseph, Beena Mathew
The synthesis of metal nanoparticles by green methods attained enormous attention in recent years due to its easiness, non-toxicity, and eco-friendly nature. In the present study, noble metal nanoparticles such as silver and gold were prepared using an aqueous leaf extract of a medicinal plant, Bauhinia purpurea. The leaf extract performed as both reducing and stabilizing agents for the development of nanoparticles. The formations of silver and gold nanoparticles were confirmed by observing the surface plasmon resonance peaks at 430 nm and 560 nm, respectively, in UV-Vis absorption spectrum...
November 12, 2018: Bioprocess and Biosystems Engineering
Zou Bin, Chu Yanhong, Xia Jiaojiao
A novel and highly stable biomimetic oxidase sensor system was designed for catehol detection. FePP used as biomimetic horseradish peroxidase (HRP) was immobilized onto modified multi-walled carbon nanotubes (MWCNTs). Functional groups such as -OH, -NH2 and -COOH were introduced onto the surface of MWCNTs to provide biomimetic microenvironment for iron porphyrins (FePP). Stable biomimetic enzyme electrode has been developed to detect catechol as a simple, economical and efficient method. At optimal condition, the detection limit of OH-MWCNTs/FePP/Nafion was 3...
November 12, 2018: Bioprocess and Biosystems Engineering
Mesfin Geberekidan, Jian Zhang, Z Lewis Liu, Jie Bao
A low-cost and sustainable cellulosic ethanol production is vital for fermentation-based industrial applications. Reducing the expenses of cellulose-deconstruction enzymes is one of the significant challenges to economic cellulose-to-ethanol conversion. Here, we report the improved ethanol production from corn stover after dry biorefining using a natural β-glucosidase-producing strain Clavispora NRRL Y-50464 with a low cellulase dose of 5 mg protein/g glucan under separate enzymatic hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) conditions...
November 8, 2018: Bioprocess and Biosystems Engineering
Katarína Boriová, Slavomír Čerňanský, Peter Matúš, Marek Bujdoš, Alexandra Šimonovičová, Martin Urík
This paper provides a unique comparison of the performance of four wild-type Aspergillus niger strains in remediation of aluminium(III)-contaminated aqueous solutions. The direct fungal aluminium removal via biosorption and bioaccumulation was compared among all fungal strains, including bioaccumulation efficiency during dynamic and static cultivation. Our results indicate that aluminium bioaccumulation by living biomass outperformed biosorption, although biosorption by non-living biomass is a less time-demanding process...
November 7, 2018: Bioprocess and Biosystems Engineering
Daniel Borchert, Diego A Suarez-Zuluaga, Patrick Sagmeister, Yvonne E Thomassen, Christoph Herwig
Root cause analysis (RCA) is one of the most prominent tools used to comprehensively evaluate a biopharmaceutical production process. Despite of its widespread use in industry, the Food and Drug Administration has observed a lot of unsuitable approaches for RCAs within the last years. The reasons for those unsuitable approaches are the use of incorrect variables during the analysis and the lack in process understanding, which impede correct model interpretation. Two major approaches to perform RCAs are currently dominating the chemical and pharmaceutical industry: raw data analysis and feature-based approach...
October 31, 2018: Bioprocess and Biosystems Engineering
Ehsan Mahdinia, Ali Demirci, Aydin Berenjian
Menaquinone-7 (MK-7) as the most important form of Vitamin K has been reported to have miraculous benefits such as preventing cardiovascular diseases and osteoporosis along with antitumor effects. Therefore, there have been numerous studies in the past decades to improve MK-7 production via microbial fermentation. Unfortunately, both solid and liquid state fermentation strategies that are utilized for MK-7 production, face fundamental operational and scale-up issues as well as intense heat and mass transfer problems during fermentation...
October 27, 2018: Bioprocess and Biosystems Engineering
Daniela Bresolin, Arthur S Estrella, Jacqueline R P da Silva, Alexsandra Valério, Cláudia Sayer, Pedro H H de Araújo, Débora de Oliveira
The use of green sources for materials synthesis has gained popularity in recent years. This work investigated the immobilization of lipase NS-40116 (Thermomyces lanuginosus lipase) in polyurethane foam (PUF) using a biopolyol obtained through the enzymatic glycerolysis between castor oil and glycerol, catalyzed by the commercial lipase Novozym 435 for the PUF formation. The reaction was performed to obtain biopolyol resulting in the conversion of 64% in mono- and diacylglycerol, promoting the efficient use of the reaction product as biopolyol to obtain polyurethane foam...
October 26, 2018: Bioprocess and Biosystems Engineering
Jordi Gabarró, Miriam Guivernau, Laura Burgos, Oswald Garanto, August Bonmatí
A biological aerated filter (BAF) pilot plant consisting of two reactors (aerobic and anoxic one) was used to determine a strategy to remove nitrogen via nitrite. RNA/DNA analysis was performed to assess microbial activity and support chemical results. In less than 13 days the pilot plant was able to remove COD and suspended solids. Nitrogen removal via nitrite pathway could not be observed until day 130 when the empty bed contact time (EBCT) was set at 0.71 h. Nitrite was detected in the aerated BAF effluent but never nitrate...
October 26, 2018: Bioprocess and Biosystems Engineering
Dharmesh H Sur, Mausumi Mukhopadhyay
The biological treatment of textile effluent is enhanced by the use of zinc oxide (ZnO) nanoparticles for the reduction in chemical oxygen demand (COD) from its initial value of 1700 ppm. The present research investigated the effect of ZnO nanoparticles when microbial cultures of Pseudomonas putida and Pseudomonas aureofaciens were used to treat textile effluent in three-phase inverse fluidized bed bioreactor. The parameters like-size of ZnO nanoparticles, static bed height, superficial gas velocities and solid media particle size-together affected the COD reduction and all of these were investigated in this paper...
October 25, 2018: Bioprocess and Biosystems Engineering
Sirwan Khanchezar, Sameereh Hashemi-Najafabadi, Seyed Abbas Shojaosadati, Valiollah Babaeipour
Miniaturized bubble columns (MBCs) have different hydrodynamics in comparison with the larger ones, but there is a lack of scientific data on MBCs. Hence, in this study, the effect of gas hold-up, flow regimes, bubble size distribution on volumetric oxygen mass transfer coefficient at different pore size spargers and gas flow rates in MBCs in the presence and absence of microorganisms were investigated. It was found that flow regime transition occurred around low gas flow rates of 1.18 and 0.85 cm/s for small (16-40 µm) and large (40-100 µm) pore size spargers, respectively...
October 25, 2018: Bioprocess and Biosystems Engineering
Xiyan Ji, Yanyun Liu, Jibiao Zhang, Deying Huang, Peiguo Zhou, Zheng Zheng
A pilot-scale deep bed denitrification filter using quartz sand as the filter media was operated under filtration velocity of 5.23 m/h. Nitrate, nitrite, ammonia, and total nitrogen removal rates were relatively high at influent C/N ratios of 4:1 and 5:1. A model was developed using software to simulate the processes operating in the filter and improve the related parameters in the actual operations. The normalized sensitivity coefficient and the mean square sensitivity measure were used for the sensitivity analysis...
October 23, 2018: Bioprocess and Biosystems Engineering
José María Encinar, Juan Félix González, Nuria Sánchez, Sergio Nogales-Delgado
The transesterification of sunflower oil with methanol, using immobilized lipase enzymes as catalysts, was studied. The process was carried out in a semi-continuous mode. Temperature (30-50 °C), methanol flow (0.024-0.04 ml/min), kind of enzyme (Lipozyme 62350, Lipozyme TL-IM, Novozym 435 and Pseudomonas cepacia Sol-Gel-AK) and enzyme concentrations (1.25-10% by weight) were the operating variables. The final product was characterized by the EN 14214 standard. All the parameters, except for cold filter plugging point, were similar to a diesel fuel...
October 9, 2018: Bioprocess and Biosystems Engineering
Agnieszka Tuszynska, Magdalena Kaszubowska, Przemyslaw Kowal, Slawomir Ciesielski, Jacek Makinia
The effect of distillery waste product (fusel oil) as an alternative external organic carbon source (EOCS) was investigated in terms of the metabolic properties of denitrifying polyphosphate accumulating organisms (DPAOs). Samples of the non-acclimated biomass were collected from a local full-scale wastewater treatment plant employing A2 /O type bioreactors. The acclimated biomass was obtained after cultivation (with fusel oil added) in a bench-scale reactor with a process configuration similar to the full-scale bioreactor...
October 5, 2018: Bioprocess and Biosystems Engineering
Siyuan Zhai, Yingxin Zhao, Min Ji, Wenfang Qi
A dicyclic-type electrode-based biofilm-electrode reactor (BER) was investigated for simultaneous removal of nitrate and Cr(VI). In the absence of Cr(VI), almost complete denitrification of 50 mg/L NO3 - -N was achieved at a very low C/N ratio of 0.8 with the optimal current of 50 mA. Cr(VI) was removed by biological reduction and co-precipitation when Cr(VI) was taken as the only electron acceptor, and the removal efficiencies of Cr(VI) were 99.8%. In the coexistent system of nitrate and Cr(VI), nitrate removal was the result of the cooperation of hydrogenotrophic denitrification and heterotrophic denitrification...
October 5, 2018: Bioprocess and Biosystems Engineering
Chadathorn Inyai, Panitch Boonsnongcheep, Jukrapun Komaikul, Boonchoo Sritularak, Hiroyuki Tanaka, Waraporn Putalun
Morus alba L. (Moraceae) has been used in traditional medicine for the treatment of several illnesses. Recent research also revealed several pharmacological activities from many groups of secondary metabolites, including the stilbenoids mulberroside A, oxyresveratrol, and resveratrol, which are promising compounds for cosmetic and herbal supplement products. In our previous study, cell cultures of M. alba showed high productivity of these compounds. In this study, we attempted to develop immobilized cell cultures of M...
October 3, 2018: Bioprocess and Biosystems Engineering
Luis C Reyes-Alvarado, Artin Hatzikioseyian, Eldon R Rene, Eric Houbron, Elena Rustrian, Giovanni Esposito, Piet N L Lens
Biological reduction of sulphate at low hydraulic retention time (HRT) is presented in this paper. A sulphidogenic inverse fluidized-bed bioreactor (IFBB) was operated successfully at a progressively decreasing HRT from 1 to 0.125 days for a total of 155 days. Synthetic wastewater containing sulphate at a concentration of 745 (± 17) mg/L was used. COD was supplied as lactate in variable concentrations at COD/SO4 2- ratios of 1.2-2.4. The pH of the feed ranged between 5.2 and 6.2. The highest measured removal rates were 2646 and 4866 mg SO4 2- /L day at an HRT of 0...
September 29, 2018: Bioprocess and Biosystems Engineering
Lingjun Geng, Shenglan Chen, Xiaoman Sun, Xuechao Hu, Xiaojun Ji, He Huang, Lujing Ren
The ω-3/long-chain polyunsaturated fatty acids (LC-PUFAs) play an important role in human health, but they cannot be synthesized in sufficient amounts by the human body. In a previous study, we obtained an engineered Schizochytrium sp. strain (HX-RS) by exchanging the acyltransferase (AT) gene, and it was able to co-produce docosahexaenoic acid and eicosapentaenoic acid. To investigate the mechanism underlying the increase of PUFA content in HX-RS, the discrepancies of fermentation performance, key enzyme activities and intracellular metabolites between HX-RS and its wild-type parent strain (WTS) were analyzed via fed-batch fermentation in 5-L bioreactors...
September 28, 2018: Bioprocess and Biosystems Engineering
Fernanda Roberta Rech, Roselei Claudete Fontana, Carlos A Rosa, Marli Camassola, Marco Antônio Záchia Ayub, Aldo J P Dillon
The present study evaluated 13 strains of yeast for ethanol and xylitol production from xylose. Among them, Spathaspora hagerdaliae UFMG-CM-Y303 produced ethanol yields (YP/S ) of 0.25 g g- 1 and 0.39 g g- 1 under aerobic and microaerophilic conditions, respectively, from a mixture of glucose and xylose in flasks. A pH of 5.0 and an inoculum of 3.0 × 108 cells mL- 1 r resulted in the highest ethanol yields. These conditions were tested in a bioreactor for fermenting a medium containing an enzymatic hydrolysate of sugarcane bagasse with 15...
September 27, 2018: Bioprocess and Biosystems Engineering
Abdullah Amru Indera Luthfi, Jian Ping Tan, Shuhaida Harun, Shareena Fairuz Abdul Manaf, Jamaliah Md Jahim
Continuous bio-production of succinic acid was reported in homogeneous solid dispersion (HSD) system utilizing porous coconut shell activated carbon (CSAC) as immobilization carrier. The aim of the present work was to implement the HSD system to increase the area of cell immobilization and the rate of succinic-acid production from the lignocellulosic medium. The ratio of the two enzymes (cellulase-to-hemicellulase) was initially optimized to break down the lignocellulose into its free monomers, wherein the best ratio was determined as 4:1...
September 27, 2018: Bioprocess and Biosystems Engineering
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