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Bioprocess and Biosystems Engineering

Holger Morschett, Danny Schiprowski, Jannis Rohde, Wolfgang Wiechert, Marco Oldiges
Extended cultivation times, rendering phototrophic bioprocess development time inefficient, resulted in the recent development of micro-photobioreactors enabling accelerated process development. However, especially for laboratory photobioreactors, only little is known concerning the influence of design on process performance. Thus, the aim of the present investigation was to evaluate the scalability of a microtiter plate-based parallelized micro-photobioreactor against a representative set of established laboratory photobioreactors...
January 18, 2017: Bioprocess and Biosystems Engineering
D Tafoukt, A Soric, J-C Sigoillot, J-H Ferrasse
The competitiveness of the second-generation bioethanol by biotechnological process requires an effective and quantitative control of biochemical reactions. In this study, the potential of isothermal calorimetry technique to measure heat and kinetics of a non-homogeneous substrate enzymatic hydrolysis is intended. Using this technique, optimum temperature of the enzymes used for lignocellulosic molecules hydrolysis was determined. Thus, the amount of substrate-to-enzyme ratio was highlighted as an important parameter of the hydrolysis yield...
January 6, 2017: Bioprocess and Biosystems Engineering
M A Valderrama-Gomez, D Kreitmayer, S Wolf, A Marin-Sanguino, A Kremling
Computational methods have enabled the discovery of non-intuitive strategies to enhance the production of a variety of target molecules. In the case of succinate production, reviews covering the topic have not yet analyzed the impact and future potential that such methods may have. In this work, we review the application of computational methods to the production of succinic acid. We found that while a total of 26 theoretical studies were published between 2002 and 2016, only 10 studies reported the successful experimental implementation of any kind of theoretical knowledge...
December 31, 2016: Bioprocess and Biosystems Engineering
Jung-Eun Lee, Yadhu N Guragain, Krishna P Bastola, Praveen V Vadlani
Sorghum stalks were evaluated to generate clean sugars; enzymatic hydrolysis efficiency of acid pretreated biomass was significantly low (12.3 g sugar/g pretreated biomass) compared to alkali method (49.1 g sugar/g pretreated biomass). Inhibitory compounds derived from acid pretreatment, such as hydroxymethyl furfural (HMF) and furfural, were effectively removed using an appropriate detoxification method using 1% (w/v) activated charcoal. The resin mixture containing cationic exchangers (DOWEX 50WX8) and anionic exchangers (IRA 743) in 7:3 ratio at pH 2...
December 31, 2016: Bioprocess and Biosystems Engineering
Ranjitha R Prabhu, Deepak Parashar, T Satyanarayana
The recombinant alkalistable and moderately thermostable bifunctional endoglucanase gene (BhCell-Xyl) of polyextremophilic bacterium Bacillus halodurans TSLV1 has been expressed in Pichia pastoris under constitutive GAP as well as inducible AOX promoters. A higher titre of recombinant BhCell-Xyl was attained after induction (4.8 U mL(-1)) as compared to that of the constitutive production (2.1 U mL(-1)). The recombinant P. pastoris strains integrated two copies of BhCell-Xyl under AOX and GAP promoters...
December 30, 2016: Bioprocess and Biosystems Engineering
Pornkamol Unrean
We have previously developed a dynamic flux balance analysis of Saccharomyces cerevisiae for elucidation of genome-wide flux response to furfural perturbation (Unrean and Franzen, Biotechnol J 10(8):1248-1258, 2015). Herein, the dynamic flux distributions were analyzed by flux control analysis to identify target overexpressed genes for improved yeast robustness against furfural. The flux control coefficient (FCC) identified overexpressing isocitrate dehydrogenase (IDH1), a rate-controlling flux for ethanol fermentation, and dicarboxylate carrier (DIC1), a limiting flux for cell growth, as keys of furfural-resistance phenotype...
December 26, 2016: Bioprocess and Biosystems Engineering
Malin Hultberg, Olle Lind, Göran Birgersson, Håkan Asp
The effluent from the biogas process was tested as a nutrient source during cultivation of the protein-rich and edible microalgae Spirulina (Arthrospira platensis) and compared with conventional Spirulina medium. Equal biomass production was observed until late exponential phase and no significant differences could be observed between the treatments in protein amount, amino acid composition, and total lipid concentration. The concentration of the pigment phycocyanin differed significantly between Spirulina medium and the effluent-based medium (63...
December 26, 2016: Bioprocess and Biosystems Engineering
Zeina Baalbaki, Elena Torfs, Thomas Maere, Viviane Yargeau, Peter A Vanrolleghem
The presence of micropollutants in the environment has triggered research on quantifying and predicting their fate in wastewater treatment plants (WWTPs). Since the removal of micropollutants is highly related to conventional pollutant removal and affected by hydraulics, aeration, biomass composition and solids concentration, the fate of these conventional pollutants and characteristics must be well predicted before tackling models to predict the fate of micropollutants. In light of this, the current paper presents the dynamic modelling of conventional pollutants undergoing activated sludge treatment using a limited set of additional daily composite data besides the routine data collected at a WWTP over one year...
December 24, 2016: Bioprocess and Biosystems Engineering
María Luisa Alvarado-Gutiérrez, Nora Ruiz-Ordaz, Juvencio Galíndez-Mayer, Fortunata Santoyo-Tepole, Everardo Curiel-Quesada, Jaime García-Mena, Deifilia Ahuatzi-Chacón
The fungicide carbendazim is an ecotoxic agent affecting aquatic biota. Due to its suspected hormone-disrupting effects, it is considered a "priority hazard substance" by the Water Framework Directive of the European Commission, and its degradation is of major concern. In this work, a horizontal tubular biofilm reactor (HTBR) operating in plug-flow regime was used to study the kinetics of carbendazim removal by an acclimated microbial consortium. The reactor was operated in steady state continuous culture at eight different carbendazim loading rates...
December 22, 2016: Bioprocess and Biosystems Engineering
Kamal Elkahlout, Siamak Alipour, Inci Eroglu, Ufuk Gunduz, Meral Yucel
In this study, agar immobilization technique was employed for biological hydrogen production using Rhodobacter capsulatus DSM 1710 (wild type) and YO3 (hup-mutant) strains in sequential batch process. Different agar and glutamate concentrations were tested with defined nutrient medium. Agar concentration 4% (w/v) and 4 mM glutamate were selected for bacterial immobilization in terms of rate and longevity of hydrogen production. Acetate concentration was increased from 40 to 60-100 and 60 mM gave best results with both bacterial strains immobilized in 4% (w/v) agar...
December 20, 2016: Bioprocess and Biosystems Engineering
Sarada Prasanna Mallick, Amit Rastogi, Satyavrat Tripathi, Pradeep Srivastava
The current work is an attempt to study the strategies for cartilage tissue regeneration using porous scaffold in wavy walled airlift bioreactor (ALBR). Novel chitosan, poly (L-lactide) and hyaluronic acid based composite scaffold were prepared. The scaffolds were cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, N-hydroxysuccinimide and chondroitin sulfate to obtain interconnected 3D microstructure showing excellent biocompatibility, higher cellular differentiation and increased stability. The surface morphology and porosity of the scaffolds were analyzed using scanning electron microscopy (SEM) and mercury intrusion porosimeter and optimized for chondrocyte regeneration...
December 19, 2016: Bioprocess and Biosystems Engineering
Jagriti Narang, Nitesh Malhotra, Chaitali Singhal, Rishabh Bhatia, Vikas Kathuria, Manan Jain
Metformin (Mf) plays a major role in controlling insulin level of individuals at risk of developing diabetes mellitus. Overdose of Mf can cause lactic acidosis, diarrhoea, cough, or hoarseness, etc. These particulars point out the identification for selective and sensitive methods of Mf determination. In the present work, graphene nanoflakes-polymethylene blue (GNF-PMB) nano-composites were developed onto fluorine-doped tin oxide (SnO2/F) coated glass substrates for electrochemical sensing of Mf using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)...
December 19, 2016: Bioprocess and Biosystems Engineering
Trung Hau Nguyen, Chae Hun Ra, InYung Sunwoo, Gwi-Taek Jeong, Sung-Koo Kim
This study examined the pretreatment, enzymatic saccharification, and fermentation of the red macroalgae Gracilaria verrucosa using adapted saccharomyces cerevisiae to galactose or NaCl for the increase of bioethanol yield. Pretreatment with thermal acid hydrolysis to obtain galactose was carried out with 11.7% (w/v) seaweed slurry and 373 mM H2SO4 at 121 °C for 59 min. Glucose was obtained from enzymatic hydrolysis. Enzymatic saccharification was performed with a mixture of 16 U/mL Celluclast 1.5L and Viscozyme L at 45 °C for 48 h...
December 18, 2016: Bioprocess and Biosystems Engineering
Manel Boudabbous, Ines Ben Hmad, Walid Saibi, Mariem Mssawra, Hafedh Belghith, Ali Gargouri
An extracellular β-glucosidase from Fusaruim solani cultivated on wheat bran was purified by only two chromatographic steps. The purified enzyme exhibited optimal temperature and pH at 60 °C and pH 5, respectively. The purified β-glucosidase behaves as a very large protein due to its high degree of glycosylation. More interestingly, the endoglycosidase H (Endo H) treatment led to 97.55% loss of its initial activity after 24 h of treatment. Besides, the addition of Tunicamycin (nucleoside antibiotic blocking the N-glycosylation first step) during the culture of the fungus affected seriously the glycosylation of the enzyme...
December 17, 2016: Bioprocess and Biosystems Engineering
Ramakant Pandey, M Premalatha
Open raceway ponds are widely adopted for cultivating microalgae on a large scale. Working depth of the raceway pond is the major component to be analysed for increasing the volume to surface area ratio. The working depth is limited up to 5-15 cm in conventional ponds but in this analysis working depth of raceway pond is considered as 25 cm. In this work, positioning of the paddle wheel is analysed and corresponding Vertical Mixing Index are calculated using CFD. Flow pattern along the length of the raceway pond, at three different paddle wheel speeds are analysed for L/W ratio of 6, 8 and 10, respectively...
December 17, 2016: Bioprocess and Biosystems Engineering
Adriana Villegas, Juan Pablo Arias, Daira Aragón, Silvia Ochoa, Mario Arias
In this work, a mechanistic model for predicting the dynamic behavior of extracellular and intracellular nutrients, biomass production, and the main metabolites involved in the central carbon metabolism in plant cell cultures of Thevetia peruviana is presented. The proposed model is the first mechanistic model implemented for plant cell cultures of this species, and includes 28 metabolites, 33 metabolic reactions, and 61 parameters. Given the over-parametrization of the model, its nonlinear nature and the strong correlation among the effects of the parameters, a parameter estimation routine based on identifiability analysis was implemented...
December 16, 2016: Bioprocess and Biosystems Engineering
Mingke Wu, Xiaozhan Li, Shunfeng Guo, Wubliker Dessie Lemma, Wenming Zhang, Jiangfeng Ma, Honghua Jia, Hao Wu, Min Jiang, Pingkai Ouyang
Biological synthesis of succinic acid at low pH values was favored since it not only decreased investment cost but also simplified downstream purification process. In this study, the feasibility of using glutamate decarboxylase system to improve succinic acid production of Escherichia coli AFP111, a succinate-producing candidate with mutations in pfl, ldhA, and ptsG, under acidic conditions was investigated. By overexpressing gadBC operon in AFP111, a recombinant named as BA201 (AFP111/pMD19T-gadBC) was constructed...
December 16, 2016: Bioprocess and Biosystems Engineering
Janaina S Lima, Pedro H H Araújo, Claudia Sayer, Antonio A U Souza, Alexandre C Viegas, Débora de Oliveira
Immobilization of cellulases on magnetic nanoparticles, especially magnetite nanoparticles, has been the main approach studied to make this enzyme, economically and industrially, more attractive. However, magnetite nanoparticles tend to agglomerate, are very reactive and easily oxidized in air, which has strong impact on their useful life. Thus, it is very important to provide proper surface coating to avoid the mentioned problems. This study aimed to investigate the immobilization of cellulase on magnetic nanoparticles encapsulated in polymeric nanospheres...
December 10, 2016: Bioprocess and Biosystems Engineering
Diana Ivonne Franco-Medrano, Patricia Guerrero-Germán, Rosa María Montesinos-Cisneros, Jaime Ortega-López, Armando Tejeda-Mansir
The demand for plasmid DNA (pDNA) has increased in response to the rapid advances in vaccines applications to prevent and treat infectious diseases caused by virus, bacteria or parasites, such as Leishmania species. The immunization protocols require large amounts of supercoiled plasmid DNA (sc-pDNA) challenging the development of efficient and profitable processes for capturing and purified pDNA molecules from large volumes of lysates. A typical bioprocess involves four steps: fermentation, primary recovery, intermediate recovery and final purification...
December 2, 2016: Bioprocess and Biosystems Engineering
Marcos Camargo Knirsch, Filippo Dell'Anno, Marco Salerno, Claudio Larosa, Bronislaw Polakiewicz, Roberto Eggenhöffner, Attilio Converti
Polyhemoglobin produced from pure bovine hemoglobin by reaction with PEG bis(N-succynimidil succinate) as a cross-linking agent was encapsulated in gelatin and dehydrated by freeze-drying. Free carboxyhemoglobin and polyhemoglobin microcapsules were characterized by UV-Vis spectroscopy in the absorption range 450-650 nm and cyclic voltammetry in the voltage range from -0.8 to 0.6 mV to evaluate the ability to break the bond with carbon monoxide and to study the carrier's affinity for oxygen, respectively...
December 1, 2016: Bioprocess and Biosystems Engineering
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