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Bioprocess and Biosystems Engineering

Ayobami Matthew Olajuyin, Maohua Yang, Tingzhen Mu, Jiangnan Tian, Anders Thygesen, Omolola Abidemi Adesanoye, Oluwatosin Adekunle Adaramoye, Andong Song, Jianmin Xing
A biorefinery process for high yield production of succinic acid from biomass sugars was investigated using recombinant Escherichia coli. The major problem been addressed is utilization of waste biomass for the production of succinic acid using metabolic engineering strategy. Here, methanol extract of Strophanthus preussii was used for fermentation. The process parameters were optimized. Glucose (9 g/L), galactose (4 g/L), xylose (6 g/L) and arabinose (0.5 g/L) were the major sugars present in the methanol extract of S...
July 13, 2018: Bioprocess and Biosystems Engineering
Anand Govind More, Sunil Kumar Gupta
The assessment of chromium removal efficiency (CRE) was studied using experimental trials with variations in operating conditions for evaluating performance efficiency of the Bioelectrochemical system (BES). During the study, maximum CRE of 88.36 ± 8.16% was obtained at cathode pH 2 ± 0.1 from the varied cathode pH (pH 1, 2, 3, 4, 5, 6, 7). With varying reactor temperatures from 25 to 45 °C (25, 30, 35, 40, 45 °C), BES resulted in maximum CRE of 85.93 ± 9.62% at 40 °C. CRE increased from 78...
July 13, 2018: Bioprocess and Biosystems Engineering
Ming Hong, Jianguo Liao, Ju Chu
Erythromycin A is an important antibiotic. A chemically defined synthetic medium for erythromycin production was systematically optimized in this study. A high-throughput method was employed to reduce the number of components and optimize the concentration of each component. After two round single composition deletion experiment, only 19 components were remained in the medium, and then the concentration of each component was optimized through PB experiment. The optimal medium from the PB experiment was further optimized according to the nitrogen and phosphate metabolic consumption in 5 L bioreactor...
July 13, 2018: Bioprocess and Biosystems Engineering
Mustafa Evren Ersahin
Anaerobic digestion is one of the most commonly accepted processes applied for the stabilization and treatment of primary sludge generated in municipal wastewater treatment plants enabling energy recovery via biogas production. Understanding and optimizing anaerobic sludge digesters play a key role in sustainability of wastewater treatment plants. Mathematical modeling of biological treatment systems provides several advantages such as better understanding of biochemical processes, interrelations of different biomass types, and impact of environmental conditions on the treatment plant performance...
July 12, 2018: Bioprocess and Biosystems Engineering
Marc Tuczinski, Florencia Saravia, Harald Horn
For an undisturbed operation of two-stage high-pressure fermentation up to 100 bar, a particle-free hydrolysate appears to be necessary. This is even more important if the second stage, i.e., the methane reactor, is designed as fixed bed. Here, we present the potential of microfiltration membranes as separation unit after the first stage, which is the hydrolysis. The study included the selection of membrane material, membrane performance investigations, and long-term-behavior during the filtration period. In a series of experiments, the optimum type of membrane material and the mode of operation [either crossflow (CF) or submerged (S)] were determined...
July 12, 2018: Bioprocess and Biosystems Engineering
Weizhu Zeng, Sha Xu, Guocheng Du, Song Liu, Jingwen Zhou
A strategy to achieve the efficient co-production of α-ketoglutarate (KGA) and pyruvate (PYR) via Yarrowia lipolytica fermentation was established in our previous work. The next big challenge is to achieve an efficient separation of the two keto acids. A strategy for simultaneously separating and purifying KGA and PYR based on their different boiling points was established, leading to the efficient separation and purification of the two keto acids from the fermentation broth of Y. lipolytica. The purity and yield of KGA/PYR reached 99...
July 11, 2018: Bioprocess and Biosystems Engineering
Alexander Schopf, Robert Delatolla, Rochelle Mathew, Alexandra Tsitouras, Kathlyn M Kirkwood
Copper, a prevalent heavy metal in industrial mining wastewaters, has been shown to inhibit nitrification in wastewater treatment systems. Biofilm treatment systems have an inherent potential to reduce inhibition. This study investigated the effects of copper concentration on nitrifying biofilms in moving bed biofilm reactor (MBBR) systems across long term operation using influent ammonia concentrations representative of gold mining wastewater. Conventional isotherm models did not adequately model the attachment of copper to the biofilm...
July 6, 2018: Bioprocess and Biosystems Engineering
Delei Xu, Haiqing Yao, Changhong Cao, Zhaoxian Xu, Sha Li, Zheng Xu, Jiahai Zhou, Xiaohai Feng, Hong Xu
The antibacterial polymer ɛ-poly-L-lysine (ε-PL) has been widely used as a safe food preservative. As the synthesis of ε-PL requires a rich supply of nitrogen, the efficiency of nitrogen translocation and utilization is extremely important. The objective of this study was to improve the production of ε-PL by overexpressing the ammonium transporter gene amtB in Streptomyces albulus PD-1. Using the recombinant bacteria, the optimum carbon-to-nitrogen ratio in the synthesis stage of fermentation increased from 3 to 4...
July 5, 2018: Bioprocess and Biosystems Engineering
Abdi Escalante-Sánchez, Josefina Barrera-Cortés, Héctor Mario Poggi-Varaldo, Teresa Ponce-Noyola, Ieroham Solomon Baruch
On bioprocess engineering, experimental measurements are always a costly part of the modeling effort; therefore, there is a constant need to develop cheaper, simpler, and more efficient methodologies to exploit the information available. The aim of the present work was to develop a soft sensor with the capacity to perform reliable substrate predictions and control in microbial cultures of the fed-batch type, using mainly microbial growth data. This objective was achieved using dielectric spectroscopy technology for online monitoring of microbial growth and hybrid neural networks for online prediction of substrate concentration...
July 3, 2018: Bioprocess and Biosystems Engineering
Kathrin Doll, Anton Rückel, Peter Kämpf, Maximilian Wende, Dirk Weuster-Botz
Microbial batch production of alcohols by fermentation of CO-rich gases with Clostridia is limited by low volumetric productivities due to the need for formation of organic acids first (acidogenic phase) followed by re-consumption of the acids to form alcohols (solventogenic phase). Continuous autotrophic production of alcohols was made possible with C. carboxidivorans by use of two continuously operated stirred-tank bioreactors in series without cell retention. The pH in the first reactor was controlled to pH 6...
July 3, 2018: Bioprocess and Biosystems Engineering
Asma Gnouma, Emna Sehli, Walid Medhioub, Rym Ben Dhieb, Mahmoud Masri, Norbert Mehlmer, Wissem Slimani, Khaled Sebai, Amel Zouari, Thomas Brück, Amel Medhioub
Microalgae could be of importance for future biodiesel production as an alternative for a third generation of biofuels. To select the most appropriate strain for biodiesel production, three microalgae species, namely Isochrysis sp., Nannochloropsis maritima and Tetraselmis sp., isolated from Tunisian coast, were biochemically characterized. Initially, gas chromatography analysis showed that Isochrysis sp. and N. maritima contained 5- and 10-fold total fatty acids, respectively, more than Tetraselmis sp. Then, the two microalgae Isochrysis sp...
June 26, 2018: Bioprocess and Biosystems Engineering
Wubliker Dessie, Junru Zhu, Fengxue Xin, Wenming Zhang, Youming Jiang, Hao Wu, Jiangfeng Ma, Min Jiang
Coffee husk (CH), a waste obtained from processing of coffee cherries via dry method, causes serious environmental problems. In this study, strategies were designed to utilize CH for succinic acid (SA) production. Three different CH hydrolysis methods: thermal, thermochemical and crude enzymes obtained by solid state fermentation of Aspergillus niger and Trichoderma reesei, were evaluated to generate fermentable feedstock for SA production using Actinobacillus succinogenes. The feasibility of these pretreatment methods was investigated...
June 26, 2018: Bioprocess and Biosystems Engineering
Xiujuan Chen, Cheng Zhou, Yanfen Xue, Jinsong Shi, Yanhe Ma
A novel alkaline protease (named AprV) gene from Vibrio sp. DA1-1 was cloned and expressed in Escherichia coli BL21 (DE3) pLysS. The sequence analysis showed the highest homology of 68% with the characterized protease from Alkalimonas collagenimarina AC40T . The recombinant AprV was purified with the molecular weight of 28 kDa. The optimum temperature and pH were determined to be 55 °C and 10.0, respectively. The enzyme activity was slightly enhanced by Ca2+ , Mg2+ , Zn2+ , Ba2+ , and, however, was highly inhibited by Sn2+ and EDTA...
June 23, 2018: Bioprocess and Biosystems Engineering
Zhaojuan Zheng, Meijuan Xia, Xuchao Fang, Ting Jiang, Jia Ouyang
Phenyllactic acid (PLA) is a high-value compound, which was usually produced by lactic acid bacteria (LAB) as biocatalysts and glucose or phenylpyruvic acid (PPA) as starting materials for PLA synthesis in previous studies. However, the PLA produced using LAB is a racemic mixture. Besides, both glucose and PPA were unsatisfactory substrates, as the former could not produce high concentrations of PLA while the latter is not a renewable and green substrate. To overcome these drawbacks, in this study, a new biotransformation process was developed for chiral PLA production from L-phenylalanine via the intermediate PPA using recombinant Escherichia coli co-expressing L-amino acid deaminase, NAD-dependent L-lactate dehydrogenase or NAD-dependent D-lactate dehydrogenase, and formate dehydrogenase...
June 22, 2018: Bioprocess and Biosystems Engineering
Md Maruf Khan, Yoon Seok Choi, Young Kyun Kim, Jin Cheol Yoo
Pectin degrading enzyme has been increasing interest in an industrial application as biocatalysts, such as juice, textile, and wine industry. Bacillus paralicheniformis CBS3, isolated from popular traditional Korean food (kimchi), produced a novel extracellular thermostable alkaline endopolygalacturonase (BPN3). In this study, BPN3 was purified to 22.04-fold with a recovery yield of 18.93% and specific activity of 2216.41 U/mg by gel filtration and anion exchange column chromatography. The molecular mass of BPN3 was approximately 53 kDa as analyzed by SDS-PAGE and pectic zymography...
June 20, 2018: Bioprocess and Biosystems Engineering
Xia Hua, Xin Zhou, Yong Xu
Bioconversion of ethylene glycol (EG) to glycolic acid (GA) by the whole-cell of Gluconobacter oxydans in an aired stirred tank reactor (ASTR) with continuous substrate feeding yielded over 220 g/L of GA. However, the bioreactor productivity declined to an unfavorable level of 0.63 g/L/h due to negative feed-back by GA which inhibited the reaction. To overcome this problem, based on results obtained from techno-economic comparative analysis, we set up a successive recycled-cell catalytic bioprocessing ASTR, and carried out five consecutive cycles stably during 240 h...
June 15, 2018: Bioprocess and Biosystems Engineering
Lu-Xi Jiang, Li-Liang Han, Hui-Ping Wang, Jun-Wei Xu, Jian-Hui Xiao
Jiangxienone produced by Cordyceps jiangxiensis exhibits significant cytotoxicity and good selectivity against various human cancer cells, especially gastric cancer cells. In this work, the effect of nitrogen deficiency on the accumulation of jiangxienone and the transcription levels of jiangxienone biosynthesis genes was studied in submerged fermentation of C. jiangxiensis. Results showed that accumulation of jiangxienone was improved under nitrogen deficiency condition. A maximal jiangxienone content of 3...
June 14, 2018: Bioprocess and Biosystems Engineering
Han Wang, Jianye Xia, Zhiming Zheng, Ying-Ping Zhuang, Xiaoping Yi, Dahe Zhang, Peng Wang
Proliferation of anchorage-dependent cells occurs after adhesion to a suitable surface. Thus, quantitative information about the force of cells adhesion to microcarriers at early culture phases is vital for scaling up such system. In this work, a newly designed shear-generating device was proposed, based on a previously proposed contraction flow device designed for suspended cells. A design equation for the new device was also proposed to correlate the generated energy dissipation rate (EDR) with the cross-sectional area and flow rate...
June 13, 2018: Bioprocess and Biosystems Engineering
Hansung Park, Minsoo Kwak, JeongWoo Seo, JeongHyun Ju, SunYeon Heo, SeungMoon Park, WonKyung Hong
Results to date suggest that microalgal Thraustochytrids family strains can be used to produce high-functional omega-3 rich oil (~ 30-70% of dry cell weight) and carotenoid-based antioxidant pigments simultaneously with value-added bioactive potential. In the present study, we describe the isolation and characterization of a new Thraustochytrid Schizochytrium sp. from the west coastal area of Korea. This newly isolated Thraustochytrid, identified as Schizochytrium sp. through 18S rRNA analysis and named SH104, simultaneously produces high levels of DHA and carotenoid-based antioxidant pigments...
June 13, 2018: Bioprocess and Biosystems Engineering
Christoph J Behrens, Nina K Krahe, Diana Linke, Ralf G Berger
A glycosidase of the basidiomycete Bjerkandera adusta (BadGluc) was found in screenings to possess a strong decolorizing ability towards malvidin-3-galactoside, an anthocyanin abundant in various berry fruits. The BadGluc was purified from the culture supernatant via FPLC, and the corresponding gene was identified which showed low similarity to other characterized glucosidases. Scanning the primary sequence with PROSITE no active site motif was detected. Eventually, a specific 18 aa consensus pattern was identified manually...
June 12, 2018: Bioprocess and Biosystems Engineering
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