journal
MENU ▼
Read by QxMD icon Read
search

Bioprocess and Biosystems Engineering

journal
https://www.readbyqxmd.com/read/28239774/change-of-monascus-pigment-metabolism-and-secretion-in-different-extractive-fermentation-process
#1
Gong Chen, Rui Tang, Xiaofei Tian, Peng Qin, Zhenqiang Wu
Monascus pigments that were generally produced intracellularly from Monascus spp. are important natural colorants in food industry. In this study, change of pigment metabolism and secretion was investigated through fed-batch extractive fermentation and continuous extractive fermentation. The biomass, secreting rate of pigment and total pigment yield closely correlated with the activated time of extractive fermentation as well as the composition of feeding nutrients. Metal ions played a key role in both the cell growth and pigment metabolism...
February 26, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28236148/successive-membrane-separation-processes-simplify-concentration-of-lipases-produced-by-aspergillus-niger-by-solid-state-fermentation
#2
Christian Oliveira Reinehr, Helen Treichel, Marcus Vinicius Tres, Juliana Steffens, Vandré Barbosa Brião, Luciane Maria Colla
In this study, we developed a simplified method for producing, separating, and concentrating lipases derived from solid-state fermentation of agro-industrial residues by filamentous fungi. First, we used Aspergillus niger to produce lipases with hydrolytic activity. We analyzed the separation and concentration of enzymes using membrane separation processes. The sequential use of microfiltration and ultrafiltration processes made it possible to obtain concentrates with enzymatic activities much higher than those in the initial extract...
February 25, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28224230/newly-isolated-bacillus-sp-g51-from-patagonian-wool-produces-an-enzyme-combination-suitable-for-felt-resist-treatments-of-organic-wool
#3
Martín S Iglesias, Cynthia Sequeiros, Sebastián García, Nelda L Olivera
Bacteria from Patagonian Merino wool were isolated to assess their wool-keratinolytic activity and potential for felt-resist treatments. Strains from Bacillus, Exiguobacterium, Deinococcus, and Micrococcus produced wool-degrading enzymes. Bacillus sp. G51 showed the highest wool-keratinolytic activity. LC-MS/MS analysis revealed that G51 secreted two serine proteases belonging to the peptidase family S8 (MEROPS) and a metalloprotease associated with Bacillolysin, along with other enzymes (γ-glutamyltranspeptidase and dihydrolipoyl dehydrogenases) that could be involved in reduction of keratin disulfide bonds...
February 21, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28220238/application-of-calcium-phosphate-flocculation-in-high-density-cell-culture-fluid-with-high-product-titer-of-monoclonal-antibody
#4
Guoqiang Chen, Zhiguo Su, Feng Li, Hui Fang Liu
The calcium phosphate [Ca3(PO4)2] precipitation was used for improving the clarification efficiency in harvest process of the monoclonal antibody (mAb) containing cell culture fluid (CCF) with high turbidity and product titer. The flocculation conditions (concentration, addition order of flocculants, pH, and operation time), and the effect of flocculants on the mAb physical chemical properties (such as distribution of charge variants and aggregates) and process-related impurities removal (such as DNA and CHOP) were evaluated in this study...
February 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28220237/microbial-fuel-cell-coupled-to-biohydrogen-reactor-a-feasible-technology-to-increase-energy-yield-from-cheese-whey
#5
J Wenzel, L Fuentes, A Cabezas, C Etchebehere
An important pollutant produced during the cheese making process is cheese whey which is a liquid by-product with high content of organic matter, composed mainly by lactose and proteins. Hydrogen can be produced from cheese whey by dark fermentation but, organic matter is not completely removed producing an effluent rich in volatile fatty acids. Here we demonstrate that this effluent can be further used to produce energy in microbial fuel cells. Moreover, current production was not feasible when using raw cheese whey directly to feed the microbial fuel cell...
February 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28210816/efficient-solvothermal-wet-in-situ-transesterification-of-nannochloropsis-gaditana-for-biodiesel-production
#6
Bora Kim, Yong Keun Chang, Jae W Lee
In situ transesterification of wet microalgae is a promising, simplified alternative biodiesel production process that replaces multiple operations of cell drying, extraction, and transesterification reaction. This study addresses enhanced biodiesel production from Nannochloropsis gaditana at elevated temperatures. Compared with the previously reported in situ transesterification process of conducting the reaction at a temperature ranging from 95 to 125 °C, the present work employs higher temperatures of at least 150 °C...
February 16, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28204983/application-of-acetyl-coa-acetyltransferase-atoad-in-escherichia-coli-to-increase-3-hydroxyvalerate-fraction-in-poly-3-hydroxybutyrate-co-3-hydroxyvalerate
#7
Jong-Min Jeon, Hyun-Joong Kim, Shashi Kant Bhatia, Changmin Sung, Hyung-Min Seo, Jung-Ho Kim, Hyung-Yeon Park, Dahye Lee, Christopher J Brigham, Yung-Hun Yang
Polyhydroxyalkanoate (PHA) is a family of biodegradable polymers, and incorporation of different monomers can alter its physical properties. To produce the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) containing a high level of 3-hydroxyvalerate (3HV) by altering acetyl-CoA pool levels, we overexpressed an acetyl-CoA acetyltransferase (atoAD) in an engineered E. coli strain, YH090, carrying PHA synthetic genes bktB, phaB, and phaC. It was found that, with introduction of atoAD and with propionate as a co-substrate, 3HV fraction in PHA was increased up to 7...
February 16, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28204982/influence-of-carbon-sources-on-the-viability-and-resuscitation-of-acetobacter-senegalensis-during-high-temperature-gluconic-acid-fermentation
#8
Rasoul Shafiei, Raziyeh Zarmehrkhorshid, Majid Mounir, Philippe Thonart, Frank Delvigne
Much research has been conducted about different types of fermentation at high temperature, but only a few of them have studied cell viability changes during high-temperature fermentation. In this study, Acetobacter senegalensis, a thermo-tolerant strain, was used for gluconic acid production at 38 °C. The influences of different carbon sources and physicochemical conditions on cell viability and the resuscitation of viable but nonculturable (VBNC) cells formed during fermentation were studied. Based on the obtained results, A...
February 15, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28197730/mechanisms-of-the-inhibition-of-enzymatic-hydrolysis-of-waste-pulp-fibers-by-calcium-carbonate-and-the-influence-of-nonionic-surfactant-for-mitigation
#9
Byeong Cheol Min, Bandaru V Ramarao
Recycled paper mills produce large quantities of fibrous rejects and fines which are usually sent to landfills as solid waste. These cellulosic materials can be enzymatically hydrolyzed into sugars for the production of biofuels and biomaterials. Paper mill wastes also contain large amounts of calcium carbonate which inhibits cellulase activity. The calcium carbonate (30%, w/w) decreased 40-60% of sugar yield of unbleached softwood kraft pulp. The prime mechanisms for this are by pH variation, competitive and non-productive binding, and aggregation effect...
February 14, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28180999/biotic-conversion-of-sulphate-to-sulphide-and-abiotic-conversion-of-sulphide-to-sulphur-in-a-microbial-fuel-cell-using-cobalt-oxide-octahedrons-as-cathode-catalyst
#10
Pritha Chatterjee, M M Ghangrekar, Surampalli Rao, Senthil Kumar
Varying chemical oxygen demand (COD) and sulphate concentrations in substrate were used to determine reaction kinetics and mass balance of organic matter and sulphate transformation in a microbial fuel cell (MFC). MFC with anodic chamber volume of 1 L, fed with wastewater having COD of 500 mg/L and sulphate of 200 mg/L, could harvest power of 54.4 mW/m(2), at a Coulombic efficiency of 14%, with respective COD and sulphate removals of 90 and 95%. Sulphide concentration, even up to 1500 mg/L, did not inhibit anodic biochemical reactions, due to instantaneous abiotic oxidation to sulphur, at high inlet sulphate...
February 8, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28168528/rapid-start-up-of-nitrifying-mbbrs-at-low-temperatures-nitrification-biofilm-response-and-microbiome-analysis
#11
Bradley Young, Robert Delatolla, Turki Abujamel, Kevin Kennedy, Edith Laflamme, Alain Stintzi
The moving bed biofilm reactor (MBBR), operated as a post carbon removal system, requires long start-up times in comparison to carbon removal systems due to slow growing autotrophic organisms. This study investigates the use of carriers seeded in a carbon rich treatment system prior to inoculation in a nitrifying MBBR system to promote the rapid development of nitrifying biofilm in an MBBR system at temperatures between 6 and 8 °C. Results show that nitrification was initiated by the carbon removal carriers after 22 h of operation...
February 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28144742/comment-on-elucidating-the-binding-efficacy-of-%C3%AE-galactosidase-on-graphene-by-docking-approach-and-its-potential-application-in-galacto-oligosaccharide-production
#12
Marek Wiśniewski, Katarzyna Roszek
In a recent study, Satar et al. (Bioprocess Biosyst Eng 39:807-814, 2016) have reported "the synthesis and characterization of graphene for the immobilization of β-galactosidase for improved galacto-oligosaccharide (GOS) production". There are several issues in this study that must be commented.
January 31, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28120126/a-high-throughput-screening-procedure-for-enhancing-pyruvate-production-in-candida-glabrata-by-random-mutagenesis
#13
Zhengshan Luo, Weizhu Zeng, Guocheng Du, Song Liu, Fang Fang, Jingwen Zhou, Jian Chen
Candida glabrata is one of the most promising pyruvate producers. A series of experiments was conducted to enhance pyruvate production by C. glabrata via metabolic and genetic engineering. Here, a novel screening strategy, which combined atmospheric and room temperature plasma-based random mutagenesis and high-throughput screening (HTS), was used to screen for high pyruvate-producing mutants that could use cheap industrial raw materials as nitrogen sources. A high-titer pyruvate producer (H6) was obtained form 30,000 mutants after 30 rounds of mutagenesis and HTS...
January 24, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28120125/enhanced-ethanol-fermentation-by-engineered-saccharomyces-cerevisiae-strains-with-high-spermidine-contents
#14
Sun-Ki Kim, Jung-Hyun Jo, Yong-Su Jin, Jin-Ho Seo
Construction of robust and efficient yeast strains is a prerequisite for commercializing a biofuel production process. We have demonstrated that high intracellular spermidine (SPD) contents in Saccharomyces cerevisiae can lead to improved tolerance against various fermentation inhibitors, including furan derivatives and acetic acid. In this study, we examined the potential applicability of the S. cerevisiae strains with high SPD contents under two cases of ethanol fermentation: glucose fermentation in repeated-batch fermentations and xylose fermentation in the presence of fermentation inhibitors...
January 24, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28120124/metabolic-engineering-of-bacillus-subtilis-for-biosynthesis-of-heparosan-using-heparosan-synthase-from-pasteurella-multocida-pmhs1
#15
Xiaofei Chen, Rong Chen, Xiaoxiao Yu, Dongyang Tang, Wenbing Yao, Xiangdong Gao
Heparosan, the capsular polysaccharide discovered in many pathogenic bacteria, is a promising material for heparin preparation. In this study, the Pasteurella multocida heparosan synthase 1 (PmHS1) module was used to synthesize heparosan with controlled molecular weight, while tuaD/gtaB module or gcaD module was responsible for UDP-precursors production in Bacillus subtilis 168. After metabolic pathway optimization, the yield of heparosan was as high as 237.6 mg/L in strain containing PmHS1 module and tuaD/gtaB module, which indicated that these two modules were key factors in heparosan production...
January 24, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28101644/comparative-evaluation-of-phototrophic-microtiter-plate-cultivation-against-laboratory-scale-photobioreactors
#16
Holger Morschett, Danny Schiprowski, Jannis Rohde, Wolfgang Wiechert, Marco Oldiges
Extended cultivation times, rendering phototrophic bioprocess development time inefficient, resulted in the recent development of micro-photobioreactors enabling accelerated process development. However, especially for laboratory photobioreactors, only little is known concerning the influence of design on process performance. Thus, the aim of the present investigation was to evaluate the scalability of a microtiter plate-based parallelized micro-photobioreactor against a representative set of established laboratory photobioreactors...
January 18, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28062914/determination-of-kinetics-and-heat-of-hydrolysis-for-non-homogenous-substrate-by-isothermal-calorimetry
#17
D Tafoukt, A Soric, J-C Sigoillot, J-H Ferrasse
The competitiveness of the second-generation bioethanol by biotechnological process requires an effective and quantitative control of biochemical reactions. In this study, the potential of isothermal calorimetry technique to measure heat and kinetics of a non-homogeneous substrate enzymatic hydrolysis is intended. Using this technique, optimum temperature of the enzymes used for lignocellulosic molecules hydrolysis was determined. Thus, the amount of substrate-to-enzyme ratio was highlighted as an important parameter of the hydrolysis yield...
January 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28040871/application-of-theoretical-methods-to-increase-succinate-production-in-engineered-strains
#18
REVIEW
M A Valderrama-Gomez, D Kreitmayer, S Wolf, A Marin-Sanguino, A Kremling
Computational methods have enabled the discovery of non-intuitive strategies to enhance the production of a variety of target molecules. In the case of succinate production, reviews covering the topic have not yet analyzed the impact and future potential that such methods may have. In this work, we review the application of computational methods to the production of succinic acid. We found that while a total of 26 theoretical studies were published between 2002 and 2016, only 10 studies reported the successful experimental implementation of any kind of theoretical knowledge...
December 31, 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28040870/innovative-methods-to-generate-clean-sugar-stream-from-biomass-feedstocks-for-efficient-fermentation
#19
REVIEW
Jung-Eun Lee, Yadhu N Guragain, Krishna P Bastola, Praveen V Vadlani
Sorghum stalks were evaluated to generate clean sugars; enzymatic hydrolysis efficiency of acid pretreated biomass was significantly low (12.3 g sugar/g pretreated biomass) compared to alkali method (49.1 g sugar/g pretreated biomass). Inhibitory compounds derived from acid pretreatment, such as hydroxymethyl furfural (HMF) and furfural, were effectively removed using an appropriate detoxification method using 1% (w/v) activated charcoal. The resin mixture containing cationic exchangers (DOWEX 50WX8) and anionic exchangers (IRA 743) in 7:3 ratio at pH 2...
December 31, 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28039507/production-and-characteristics-of-the-recombinant-extracellular-bifunctional-endoglucanase-of-the-polyextremophilic-bacterium-bacillus-halodurans-and-its-applicability-in-saccharifying-agro-residues
#20
Ranjitha R Prabhu, Deepak Parashar, T Satyanarayana
The recombinant alkalistable and moderately thermostable bifunctional endoglucanase gene (BhCell-Xyl) of polyextremophilic bacterium Bacillus halodurans TSLV1 has been expressed in Pichia pastoris under constitutive GAP as well as inducible AOX promoters. A higher titre of recombinant BhCell-Xyl was attained after induction (4.8 U mL(-1)) as compared to that of the constitutive production (2.1 U mL(-1)). The recombinant P. pastoris strains integrated two copies of BhCell-Xyl under AOX and GAP promoters...
December 30, 2016: Bioprocess and Biosystems Engineering
journal
journal
39187
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"