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Bioprocess and Biosystems Engineering

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https://www.readbyqxmd.com/read/28447169/modeling-of-carbon-dioxide-mass-transfer-behavior-in-attached-cultivation-photobioreactor-using-the-analysis-of-the-ph-profiles
#1
Chunli Ji, Junfeng Wang, Runzhi Li, Tianzhong Liu
The CO2 mass transfer model associated with growth kinetics of microalgal biofilm in attached cultivation photobioreactor was developed and verified by using the analysis of pH profiles which were in equilibrium with inorganic carbon components concentrations (CO2, H2CO3, HCO3(-) and CO3(2-)) in medium. Model simulation results showed that the model well presented the biofilm growth process. The overall volumetric mass transfer coefficient of CO2 was more influenced by CO2 concentration in aerated gas but less by gas aeration rate and medium circulation rate...
April 26, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28447168/effect-of-carbon-sources-on-the-growth-and-ethanol-production-of-native-yeast-pichia-kudriavzevii-itv-s42-isolated-from-sweet-sorghum-juice
#2
L E Díaz-Nava, N Montes-Garcia, J M Domínguez, M G Aguilar-Uscanga
The importance of non-Saccharomyces yeast species in fermentation processes is widely acknowledged. Within this group, Pichia kudriavzevii ITV-S42 yeast strain shows particularly desirable characteristics for ethanol production. Despite this fact, a thorough study of the metabolic and kinetic characteristics of this strain is currently unavailable. The aim of this work is to study the nutritional requirements of Pichia kudriavzevii ITV-S42 strain and the effect of different carbon sources on the growth and ethanol production...
April 26, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28439671/tobacco-etch-virus-protease-mediating-cleavage-of-the-cellulose-binding-module-tagged-colored-proteins-immobilized-on-the-regenerated-amorphous-cellulose
#3
Xuelian Yu, Jiaqi Sun, Weiyu Wang, Li Jiang, Ruyue Wang, Wenjun Xiao, Beijiu Cheng, Jun Fan
In this study, four fusion proteins were designed, in which the N-terminal cellulose-binding module as the affinity tag was immobilized on the regenerated amorphous cellulose (RAC), and the release of the C-terminal colored proteins was detected easily and rapidly after on-resin cleavage using the free tobacco etch virus protease (TEVp) variant, or the immobilized cognate protease with a binding capacity of up to 220 mg protein per gram of RAC. The enhanced stability and repetitive use of the immobilized TEVp compensated slight loss of the catalytic efficiency toward the soluble protein substrate...
April 24, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28432429/enhanced-production-of-recombinant-proteins-by-a-small-molecule-protein-synthesis-enhancer-in-combination-with-an-antioxidant-in-recombinant-chinese-hamster-ovary-cells
#4
Joseph Camire, Dongjoo Kim, Soonjo Kwon
The improvement in the production of recombinant proteins has been linked in a number of small molecules such as carboxylic acids to the inhibition of histone deacetylase, leading to increased transcription of genes. However, carboxylic acids such as pentanoic acid and butanoic acid have been shown to promote an apoptotic response in Chinese hamster ovary (CHO) cell culture. Supplementation of cultures with antioxidants has shown the ability to reduce the apoptotic response of carboxylic acid supplementation, leading to increased therapeutic protein production...
April 21, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28409400/comprehensive-assessment-of-the-l-lysine-production-process-from-fermentation-of-sugarcane-molasses
#5
Omar Anaya-Reza, Teresa Lopez-Arenas
L-Lysine is an essential amino acid that can be produced by chemical processes from fossil raw materials, as well as by microbial fermentation, the latter being a more efficient and environmentally friendly procedure. In this work, the production process of L-lysine-HCl is studied using a systematic approach based on modeling and simulation, which supports decision making in the early stage of process design. The study considers two analysis stages: first, the dynamic analysis of the fermentation reactor, where the conversion of sugars from sugarcane molasses to L-lysine with a strain of Corynebacterium glutamicum is carried out...
April 13, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28391378/on-line-identification-of-fermentation-processes-for-ethanol-production
#6
M M Câmara, R M Soares, T Feital, P Naomi, S Oki, J M Thevelein, M Amaral, J C Pinto
A strategy for monitoring fermentation processes, specifically, simultaneous saccharification and fermentation (SSF) of corn mash, was developed. The strategy covered the development and use of first principles, semimechanistic and unstructured process model based on major kinetic phenomena, along with mass and energy balances. The model was then used as a reference model within an identification procedure capable of running on-line. The on-line identification procedure consists on updating the reference model through the estimation of corrective parameters for certain reaction rates using the most recent process measurements...
April 8, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28389850/production-and-partial-characterization-of-biosurfactant-produced-by-streptomyces-sp-r1
#7
Nor Syafirah Zambry, Adilah Ayoib, Nur Asshifa Md Noh, Ahmad Ramli Mohd Yahya
The present study focused on developing a wild-type actinomycete isolate as a model for a non-pathogenic filamentous producer of biosurfactants. A total of 33 actinomycetes isolates were screened and their extracellular biosurfactants production was evaluated using olive oil as the main substrate. Out of 33 isolates, 32 showed positive results in the oil spreading technique (OST). All isolates showed good emulsification activity (E24) ranging from 84.1 to 95.8%. Based on OST and E24 values, isolate R1 was selected for further investigation in biosurfactant production in an agitated submerged fermentation...
April 7, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28389849/high-efficient-production-of-citric-acid-by-aspergillus-niger-from-high-concentration-of-substrate-based-on-the-staged-addition-glucoamylase-strategy
#8
Baoshi Wang, Hua Li, Linghuan Zhu, Fengling Tan, Youran Li, Liang Zhang, Zhongyang Ding, Guiyang Shi
Citric acid (CA), an important platform-compound, has attracted much attention because of its broad applications and huge market demand. To solve high residual sugar at the fermentation end, we put forwarded strategy of pre-saccharification and then fermentation. Results showed that the residual total sugar decreased by 10.4% and the productivity increased by 4.0% and initially high glucose inhibited cell growth. Furthermore, commercial glucoamylase with high low-pH stability was proposed to staged-add in the fermentation process, which timely compensated enzyme loss, ensuring the glucose supply rate...
April 7, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28386667/dynamic-modeling-of-temperature-change-in-outdoor-operated-tubular-photobioreactors
#9
Dominic Deo Androga, Basar Uyar, Harun Koku, Inci Eroglu
In this study, a one-dimensional transient model was developed to analyze the temperature variation of tubular photobioreactors operated outdoors and the validity of the model was tested by comparing the predictions of the model with the experimental data. The model included the effects of convection and radiative heat exchange on the reactor temperature throughout the day. The temperatures in the reactors increased with increasing solar radiation and air temperatures, and the predicted reactor temperatures corresponded well to the measured experimental values...
April 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28382460/capillary-electrophoresis-method-for-the-analysis-of-organic-acids-and-amino-acids-in-the-presence-of-strongly-alternating-concentrations-of-aqueous-lactic-acid
#10
Hendrik Laube, Jana Boden, Roland Schneider
During the production of bio-based bulk chemicals, such as lactic acid (LA), organic impurities have to be removed to produce a ready-to-market product. A capillary electrophoresis method for the simultaneous detection of LA and organic impurities in less than 10 min was developed. LA and organic impurities were detected using a direct UV detection method with micellar background electrolyte, which consisted of borate and sodium dodecyl sulfate. We investigated the effects of electrolyte composition and temperature on the speed, sensitivity, and robustness of the separation...
April 5, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28382459/proteomic-studies-on-anti-tumor-agent-ansamitocin-p-3-producer-actinosynnema-pretiosum-in-response-to-ammonium-and-isobutanol
#11
Jinxia Lin, Jian-Jiang Zhong
Our previous work showed that the biosynthesis of ansamitocin P-3 (AP-3), an anti-tumor agent, by Actinosynnema pretiosum was depressed by ammonium but enhanced by isobutanol in the medium. Here we show proteomics analyses on A. pretiosum in different fermentation conditions with and without ammonium or isobutanol using two-dimensional electrophoresis (2-DE), matrix-assisted laser desorption/ionization, and linear ion trap quadrupole mass spectrometry. Pairwise comparison of repetitive 2-DE maps was performed to find differentially expressed spots, and eight proteins were identified as functionally annotated ones...
April 5, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28361361/in-vitro-antioxidant-and-antidiabetic-activities-of-zinc-oxide-nanoparticles-synthesized-using-different-plant-extracts
#12
Dilaveez Rehana, D Mahendiran, R Senthil Kumar, A Kalilur Rahiman
Phytofabricated green synthesis of zinc oxide (ZnO) nanoparticles using different plant extracts of Azadirachta indica, Hibiscus rosa-sinensis, Murraya koenigii, Moringa oleifera, and Tamarindus indica for biological applications has been reported. ZnO nanoparticles were also synthesized by chemical method to compare the efficiency of the green synthesized nanoparticles. FT-IR spectra confirmed the functional groups involved in the green synthesis of ZnO nanoparticles and the powder XRD patterns of the ZnO nanoparticles revealed pure wurtzite structure with preferred orientation at (100) reflection plane...
March 30, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28341913/medium-chain-length-poly-3-hydroxyalkanoates-carbon-nanotubes-composite-anode-enhances-the-performance-of-microbial-fuel-cell
#13
Y Hindatu, M S M Annuar, R Subramaniam, A M Gumel
Insufficient power generation from a microbial fuel cell (MFC) hampers its progress towards utility-scale development. Electrode modification with biopolymeric materials could potentially address this issue. In this study, medium-chain-length poly-3-hydroxyalkanoates (PHA)/carbon nanotubes (C) composite (CPHA) was successfully applied to modify the surface of carbon cloth (CC) anode in MFC. Characterization of the functional groups on the anodic surface and its morphology was carried out. The CC-CPHA composite anode recorded maximum power density of 254 mW/m(2), which was 15-53% higher than the MFC operated with CC-C (214 mW/m(2)) and pristine CC (119 mW/m(2)) as the anode in a double-chambered MFC operated with Escherichia coli as the biocatalyst...
March 25, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28341912/ralstonia-eutropha-as-a-biocatalyst-for-desulfurization-of-dibenzothiophene
#14
Azita Dejaloud, Farzaneh Vahabzadeh, Alireza Habibi
The potential of Ralstonia eutropha as a biocatalyst for desulfurization of dibenzothiophene (DBT) was studied in growing and resting cell conditions. The results of both conditions showed that sulfur was removed from DBT which accompanied by the formation of 2-hydroxybiphenyl (2-HBP). In growing cell experiments, glucose was used as an energy supplying substrate in initial concentrations of 55 mM (energy-limited) and 111 mM (energy-sufficient). The growing cell behaviors were quantitatively described using the logistic equation and maintenance concept...
March 24, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28324179/interactions-of-phosphate-solubilising-microorganisms-with-natural-rare-earth-phosphate-minerals-a-study-utilizing-western-australian-monazite
#15
Melissa K Corbett, Jacques J Eksteen, Xi-Zhi Niu, Jean-Philippe Croue, Elizabeth L J Watkin
Many microbial species are capable of solubilising insoluble forms of phosphate and are used in agriculture to improve plant growth. In this study, we apply the use of known phosphate solubilising microbes (PSM) to the release of rare-earth elements (REE) from the rare-earth phosphate mineral, monazite. Two sources of monazite were used, a weathered monazite and mineral sand monazite, both from Western Australia. When incubated with PSM, the REE were preferentially released into the leachate. Penicillum sp...
March 21, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28303325/use-of-pervaporation-process-for-the-recovery-of-aroma-compounds-produced-by-p-fermentans-in-sugarcane-molasses
#16
Suzan Cristina Rossi, Adriane Bianchi Pedroni Medeiros, Thiago André Weschenfelder, Agnes de Paula Scheer, Carlos Ricardo Soccol
Natural fruity aroma was produced during submerged fermentation by Pichia fermentans using sugarcane molasses as a cultivation broth. The aroma compounds were recovered from the fermentation by a pervaporation process using a polydimethylsiloxane membrane (Pervap 4060-Sulzer). Isoamyl acetate, a characteristic compound associated with fruity aromas, was the major compound produced. The pervaporation module was fed at three different temperatures to test the best conditions to recover the natural fruity aroma...
March 17, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28299465/extraction-of-microalgae-derived-lipids-with-supercritical-carbon-dioxide-in-an-industrial-relevant-pilot-plant
#17
Jan Lorenzen, Nadine Igl, Marlene Tippelt, Andrea Stege, Farah Qoura, Ulrich Sohling, Thomas Brück
Microalgae are capable of producing up to 70% w/w triglycerides with respect to their dry cell weight. Since microalgae utilize the greenhouse gas CO2, they can be cultivated on marginal lands and grow up to ten times faster than terrestrial plants, the generation of algae oils is a promising option for the development of sustainable bioprocesses, that are of interest for the chemical lubricant, cosmetic and food industry. For the first time we have carried out the optimization of supercritical carbon dioxide (SCCO2) mediated lipid extraction from biomass of the microalgae Scenedesmus obliquus and Scenedesmus obtusiusculus under industrrially relevant conditions...
March 15, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28289882/simulating-a-cyclic-activated-sludge-system-by-employing-a-modified-asm3-model-for-wastewater-treatment
#18
Feng Gao, Jun Nan, Xinhui Zhang
To interpret the biological nutrient removal in a cyclic activated sludge system (CAS), a modified model was developed by combining the process of simultaneous storage and growth, and the kinetics of soluble microbial product (S SMP) and extracellular polymeric substance (X EPS) with activated sludge model no. 3 (ASM3). These most sensitive parameters were initially selected whilst parameters with low sensitivity were given values from literature. The selected parameters were then calibrated on an oxygen uptake rate test and a batch CAS reactor on an operational cycle...
March 13, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28285455/nitrile-hydrolyzing-enzyme-from-meyerozyma-guilliermondii-and-its-potential-in-biosynthesis-of-3-hydroxypropionic-acid
#19
Qiang Zhang, Jin-Song Gong, Ting-Ting Dong, Ting-Ting Liu, Heng Li, Wen-Fang Dou, Zhen-Ming Lu, Jin-Song Shi, Zheng-Hong Xu
3-Hydroxypropionic acid (3-HP) is an important platform chemical in organic synthesis. Traditionally, 3-HP was produced by chemical methods and fermentation process. In this work, a novel enzymatic method was developed for green synthesis of 3-HP. A yeast strain harboring nitrile-hydrolyzing enzyme was newly isolated from environmental samples using 3-hydroxypropionitrile (3-HPN) as the sole nitrogen source. It was identified to be Meyerozyma guilliermondii CGMCC12935 by sequencing of the 18S ribosomal DNA and internal transcribed spacer, together with analysis of the morphology characteristics...
March 11, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28265745/investigation-of-deactivation-thermodynamics-of-lipase-immobilized-on-polymeric-carrier
#20
Kirtikumar C Badgujar, Bhalchandra M Bhanage
In the present work, we have investigated biochemical thermo-kinetic stability of lipases immobilized on a biocompatible polymeric material. Immobilization of lipase Candida rugosa (CRL) was carried out on biocompatible blend of poly vinyl alcohol (PVA) and chitosan (CHY) support via entrapment and glutardehyde (Glu) cross-linking method to produce PVA:CHY:CRL and PVA:CHY:Glu:CRL as robust biocatalyst. These immobilized lipases were characterized by various physico-biochemical characterization techniques. Later on, thermal and solvent stability of polymer immobilized lipase was determined in term of half-life time (t 0...
March 6, 2017: Bioprocess and Biosystems Engineering
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