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Bioprocess and Biosystems Engineering

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https://www.readbyqxmd.com/read/28324179/interactions-of-phosphate-solubilising-microorganisms-with-natural-rare-earth-phosphate-minerals-a-study-utilizing-western-australian-monazite
#1
Melissa K Corbett, Jacques J Eksteen, Xi-Zhi Niu, Jean-Philippe Croue, Elizabeth L J Watkin
Many microbial species are capable of solubilising insoluble forms of phosphate and are used in agriculture to improve plant growth. In this study, we apply the use of known phosphate solubilising microbes (PSM) to the release of rare-earth elements (REE) from the rare-earth phosphate mineral, monazite. Two sources of monazite were used, a weathered monazite and mineral sand monazite, both from Western Australia. When incubated with PSM, the REE were preferentially released into the leachate. Penicillum sp...
March 21, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28303325/use-of-pervaporation-process-for-the-recovery-of-aroma-compounds-produced-by-p-fermentans-in-sugarcane-molasses
#2
Suzan Cristina Rossi, Adriane Bianchi Pedroni Medeiros, Thiago André Weschenfelder, Agnes de Paula Scheer, Carlos Ricardo Soccol
Natural fruity aroma was produced during submerged fermentation by Pichia fermentans using sugarcane molasses as a cultivation broth. The aroma compounds were recovered from the fermentation by a pervaporation process using a polydimethylsiloxane membrane (Pervap 4060-Sulzer). Isoamyl acetate, a characteristic compound associated with fruity aromas, was the major compound produced. The pervaporation module was fed at three different temperatures to test the best conditions to recover the natural fruity aroma...
March 17, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28299465/extraction-of-microalgae-derived-lipids-with-supercritical-carbon-dioxide-in-an-industrial-relevant-pilot-plant
#3
Jan Lorenzen, Nadine Igl, Marlene Tippelt, Andrea Stege, Farah Qoura, Ulrich Sohling, Thomas Brück
Microalgae are capable of producing up to 70% w/w triglycerides with respect to their dry cell weight. Since microalgae utilize the greenhouse gas CO2, they can be cultivated on marginal lands and grow up to ten times faster than terrestrial plants, the generation of algae oils is a promising option for the development of sustainable bioprocesses, that are of interest for the chemical lubricant, cosmetic and food industry. For the first time we have carried out the optimization of supercritical carbon dioxide (SCCO2) mediated lipid extraction from biomass of the microalgae Scenedesmus obliquus and Scenedesmus obtusiusculus under industrrially relevant conditions...
March 15, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28289882/simulating-a-cyclic-activated-sludge-system-by-employing-a-modified-asm3-model-for-wastewater-treatment
#4
Feng Gao, Jun Nan, Xinhui Zhang
To interpret the biological nutrient removal in a cyclic activated sludge system (CAS), a modified model was developed by combining the process of simultaneous storage and growth, and the kinetics of soluble microbial product (S SMP) and extracellular polymeric substance (X EPS) with activated sludge model no. 3 (ASM3). These most sensitive parameters were initially selected whilst parameters with low sensitivity were given values from literature. The selected parameters were then calibrated on an oxygen uptake rate test and a batch CAS reactor on an operational cycle...
March 13, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28285455/nitrile-hydrolyzing-enzyme-from-meyerozyma-guilliermondii-and-its-potential-in-biosynthesis-of-3-hydroxypropionic-acid
#5
Qiang Zhang, Jin-Song Gong, Ting-Ting Dong, Ting-Ting Liu, Heng Li, Wen-Fang Dou, Zhen-Ming Lu, Jin-Song Shi, Zheng-Hong Xu
3-Hydroxypropionic acid (3-HP) is an important platform chemical in organic synthesis. Traditionally, 3-HP was produced by chemical methods and fermentation process. In this work, a novel enzymatic method was developed for green synthesis of 3-HP. A yeast strain harboring nitrile-hydrolyzing enzyme was newly isolated from environmental samples using 3-hydroxypropionitrile (3-HPN) as the sole nitrogen source. It was identified to be Meyerozyma guilliermondii CGMCC12935 by sequencing of the 18S ribosomal DNA and internal transcribed spacer, together with analysis of the morphology characteristics...
March 11, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28265745/investigation-of-deactivation-thermodynamics-of-lipase-immobilized-on-polymeric-carrier
#6
Kirtikumar C Badgujar, Bhalchandra M Bhanage
In the present work, we have investigated biochemical thermo-kinetic stability of lipases immobilized on a biocompatible polymeric material. Immobilization of lipase Candida rugosa (CRL) was carried out on biocompatible blend of poly vinyl alcohol (PVA) and chitosan (CHY) support via entrapment and glutardehyde (Glu) cross-linking method to produce PVA:CHY:CRL and PVA:CHY:Glu:CRL as robust biocatalyst. These immobilized lipases were characterized by various physico-biochemical characterization techniques. Later on, thermal and solvent stability of polymer immobilized lipase was determined in term of half-life time (t 0...
March 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28265744/temperature-profile-optimization-potential-for-multi-enzymatic-biopolymer-depolymerization-processes
#7
Christoph Kirse, Heiko Briesen
Optimal control of temperature was applied to a population balance model of enzymatically catalyzed depolymerization of a soluble polymer coupled with denaturation of enzyme. The reaction time required to reach a desired yield was predicted to be reduced by more than 10[Formula: see text] compared with isothermal operation. Also the yield within a given time could be increased by more than 5[Formula: see text] points. It was also possible to increase the yield and reduce the reaction time if a time-varying temperature profile was used...
March 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28258322/metabolic-engineering-of-synechocystis-sp-pcc-6803-for-enhanced-ethanol-production-based-on-flux-balance-analysis
#8
Katsunori Yoshikawa, Yoshihiro Toya, Hiroshi Shimizu
Synechocystis sp. PCC 6803 is an attractive host for bio-ethanol production due to its ability to directly convert atmospheric carbon dioxide into ethanol using photosystems. To enhance ethanol production in Synechocystis sp. PCC 6803, metabolic engineering was performed based on in silico simulations, using the genome-scale metabolic model. Comprehensive reaction knockout simulations by flux balance analysis predicted that the knockout of NAD(P)H dehydrogenase enhanced ethanol production under photoautotrophic conditions, where ammonium is the nitrogen source...
March 3, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28247098/kinetic-study-of-sulphuric-acid-hydrolysis-of-protein-feathers
#9
Sana Ben Hamad Bouhamed, Nabil Kechaou
Poultry feather keratin is the most important by-product from the poultry industry due to its abundance. Different methods have been still applied to process this by-product such as enzymatic hydrolysis which is expensive and inapplicable at the industrial level. This paper presents a study of acid hydrolysis of poultry feathers using different types of acids, sulphuric acid concentration, different temperatures and solid to liquid ratio to obtain a liquid product rich in peptides. The feathers analysis revealed a crude protein content of 88...
February 28, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28243738/sol-gel-encapsulation-of-pullulanase-in-the-presence-of-hybrid-magnetic-fe3o4-chitosan-nanoparticles-improves-thermal-and-operational-stability
#10
Jie Long, Xingfei Li, Xiaobei Zhan, Xueming Xu, Yaoqi Tian, Zhengjun Xie, Zhengyu Jin
Pullulanase was sol-gel encapsulated in the presence of magnetic chitosan/Fe3O4 nanoparticles. The resulting immobilized pullulanase was characterized by scanning electron microscopy, vibrating sample magnetometry, Fourier transform infrared spectroscopy and thermogravimetric analysis. The results showed that the addition of pullulanase created a more regular surface on the sol-gel matrix and an enhanced magnetic response to an applied magnetic field. The maximal activity retention (83.9%) and specific activity (291...
February 27, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28239774/change-of-monascus-pigment-metabolism-and-secretion-in-different-extractive-fermentation-process
#11
Gong Chen, Rui Tang, Xiaofei Tian, Peng Qin, Zhenqiang Wu
Monascus pigments that were generally produced intracellularly from Monascus spp. are important natural colorants in food industry. In this study, change of pigment metabolism and secretion was investigated through fed-batch extractive fermentation and continuous extractive fermentation. The biomass, secreting rate of pigment and total pigment yield closely correlated with the activated time of extractive fermentation as well as the composition of feeding nutrients. Metal ions played a key role in both the cell growth and pigment metabolism...
February 26, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28236148/successive-membrane-separation-processes-simplify-concentration-of-lipases-produced-by-aspergillus-niger-by-solid-state-fermentation
#12
Christian Oliveira Reinehr, Helen Treichel, Marcus Vinicius Tres, Juliana Steffens, Vandré Barbosa Brião, Luciane Maria Colla
In this study, we developed a simplified method for producing, separating, and concentrating lipases derived from solid-state fermentation of agro-industrial residues by filamentous fungi. First, we used Aspergillus niger to produce lipases with hydrolytic activity. We analyzed the separation and concentration of enzymes using membrane separation processes. The sequential use of microfiltration and ultrafiltration processes made it possible to obtain concentrates with enzymatic activities much higher than those in the initial extract...
February 25, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28224230/newly-isolated-bacillus-sp-g51-from-patagonian-wool-produces-an-enzyme-combination-suitable-for-felt-resist-treatments-of-organic-wool
#13
Martín S Iglesias, Cynthia Sequeiros, Sebastián García, Nelda L Olivera
Bacteria from Patagonian Merino wool were isolated to assess their wool-keratinolytic activity and potential for felt-resist treatments. Strains from Bacillus, Exiguobacterium, Deinococcus, and Micrococcus produced wool-degrading enzymes. Bacillus sp. G51 showed the highest wool-keratinolytic activity. LC-MS/MS analysis revealed that G51 secreted two serine proteases belonging to the peptidase family S8 (MEROPS) and a metalloprotease associated with Bacillolysin, along with other enzymes (γ-glutamyltranspeptidase and dihydrolipoyl dehydrogenases) that could be involved in reduction of keratin disulfide bonds...
February 21, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28220238/application-of-calcium-phosphate-flocculation-in-high-density-cell-culture-fluid-with-high-product-titer-of-monoclonal-antibody
#14
Guoqiang Chen, Zhiguo Su, Feng Li, Hui Fang Liu
The calcium phosphate [Ca3(PO4)2] precipitation was used for improving the clarification efficiency in harvest process of the monoclonal antibody (mAb) containing cell culture fluid (CCF) with high turbidity and product titer. The flocculation conditions (concentration, addition order of flocculants, pH, and operation time), and the effect of flocculants on the mAb physical chemical properties (such as distribution of charge variants and aggregates) and process-related impurities removal (such as DNA and CHOP) were evaluated in this study...
February 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28220237/microbial-fuel-cell-coupled-to-biohydrogen-reactor-a-feasible-technology-to-increase-energy-yield-from-cheese-whey
#15
J Wenzel, L Fuentes, A Cabezas, C Etchebehere
An important pollutant produced during the cheese making process is cheese whey which is a liquid by-product with high content of organic matter, composed mainly by lactose and proteins. Hydrogen can be produced from cheese whey by dark fermentation but, organic matter is not completely removed producing an effluent rich in volatile fatty acids. Here we demonstrate that this effluent can be further used to produce energy in microbial fuel cells. Moreover, current production was not feasible when using raw cheese whey directly to feed the microbial fuel cell...
February 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28210816/efficient-solvothermal-wet-in-situ-transesterification-of-nannochloropsis-gaditana-for-biodiesel-production
#16
Bora Kim, Yong Keun Chang, Jae W Lee
In situ transesterification of wet microalgae is a promising, simplified alternative biodiesel production process that replaces multiple operations of cell drying, extraction, and transesterification reaction. This study addresses enhanced biodiesel production from Nannochloropsis gaditana at elevated temperatures. Compared with the previously reported in situ transesterification process of conducting the reaction at a temperature ranging from 95 to 125 °C, the present work employs higher temperatures of at least 150 °C...
February 16, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28204983/application-of-acetyl-coa-acetyltransferase-atoad-in-escherichia-coli-to-increase-3-hydroxyvalerate-fraction-in-poly-3-hydroxybutyrate-co-3-hydroxyvalerate
#17
Jong-Min Jeon, Hyun-Joong Kim, Shashi Kant Bhatia, Changmin Sung, Hyung-Min Seo, Jung-Ho Kim, Hyung-Yeon Park, Dahye Lee, Christopher J Brigham, Yung-Hun Yang
Polyhydroxyalkanoate (PHA) is a family of biodegradable polymers, and incorporation of different monomers can alter its physical properties. To produce the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) containing a high level of 3-hydroxyvalerate (3HV) by altering acetyl-CoA pool levels, we overexpressed an acetyl-CoA acetyltransferase (atoAD) in an engineered E. coli strain, YH090, carrying PHA synthetic genes bktB, phaB, and phaC. It was found that, with introduction of atoAD and with propionate as a co-substrate, 3HV fraction in PHA was increased up to 7...
February 16, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28204982/influence-of-carbon-sources-on-the-viability-and-resuscitation-of-acetobacter-senegalensis-during-high-temperature-gluconic-acid-fermentation
#18
Rasoul Shafiei, Raziyeh Zarmehrkhorshid, Majid Mounir, Philippe Thonart, Frank Delvigne
Much research has been conducted about different types of fermentation at high temperature, but only a few of them have studied cell viability changes during high-temperature fermentation. In this study, Acetobacter senegalensis, a thermo-tolerant strain, was used for gluconic acid production at 38 °C. The influences of different carbon sources and physicochemical conditions on cell viability and the resuscitation of viable but nonculturable (VBNC) cells formed during fermentation were studied. Based on the obtained results, A...
February 15, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28197730/mechanisms-of-the-inhibition-of-enzymatic-hydrolysis-of-waste-pulp-fibers-by-calcium-carbonate-and-the-influence-of-nonionic-surfactant-for-mitigation
#19
Byeong Cheol Min, Bandaru V Ramarao
Recycled paper mills produce large quantities of fibrous rejects and fines which are usually sent to landfills as solid waste. These cellulosic materials can be enzymatically hydrolyzed into sugars for the production of biofuels and biomaterials. Paper mill wastes also contain large amounts of calcium carbonate which inhibits cellulase activity. The calcium carbonate (30%, w/w) decreased 40-60% of sugar yield of unbleached softwood kraft pulp. The prime mechanisms for this are by pH variation, competitive and non-productive binding, and aggregation effect...
February 14, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28180999/biotic-conversion-of-sulphate-to-sulphide-and-abiotic-conversion-of-sulphide-to-sulphur-in-a-microbial-fuel-cell-using-cobalt-oxide-octahedrons-as-cathode-catalyst
#20
Pritha Chatterjee, M M Ghangrekar, Surampalli Rao, Senthil Kumar
Varying chemical oxygen demand (COD) and sulphate concentrations in substrate were used to determine reaction kinetics and mass balance of organic matter and sulphate transformation in a microbial fuel cell (MFC). MFC with anodic chamber volume of 1 L, fed with wastewater having COD of 500 mg/L and sulphate of 200 mg/L, could harvest power of 54.4 mW/m(2), at a Coulombic efficiency of 14%, with respective COD and sulphate removals of 90 and 95%. Sulphide concentration, even up to 1500 mg/L, did not inhibit anodic biochemical reactions, due to instantaneous abiotic oxidation to sulphur, at high inlet sulphate...
February 8, 2017: Bioprocess and Biosystems Engineering
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