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Bioprocess and Biosystems Engineering

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https://www.readbyqxmd.com/read/28646332/a-rational-approach-for-the-improvement-of-biomass-production-and-lipid-profile-in-cacao-cell-suspensions
#1
Adriana María Gallego Rúa, Luisa Fernanda Rojas, Aura Ines Urrea Trujillo, Oriana Parra Zuleta, Cristian David Correa Alvarez, Lucía Atehortúa Garcés
Cocoa butter (CB) is produced in the seeds of Theobroma cacao representing 50% of its dry weight. The lipid composition plays an important role in the physicochemical, rheological, and sensory properties of the CB, making this fat a valuable resource for the production of chocolates, cosmetics, and pharmaceuticals. In this paper, are described experimental strategies used for a rational improvement of biomass production and fatty acids in cacao cell suspension cultures. First, the lipid profile in four cacao varieties is characterized, and then, one variety is selected to induce cell suspensions using a direct method without previous establishment of a callus phase...
June 23, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28642968/enhancing-the-enzymatic-saccharification-of-bamboo-shoot-shell-by-sequential-biological-pretreatment-with-galactomyces-sp-cczu11-1-and-deep-eutectic-solvent-extraction
#2
Yong Dai, Hai-Sheng Zhang, Bin Huan, Yucai He
In this study, sequential biological pretreatment (BP) with Galactomyces sp. CCZU11-1 at 30 °C for 3 days and deep eutectic solvent (DES) choline chloride: oxalic acid (ChCl:OA, 1 mol/2 mol) extraction at 120 °C for 1.5 h was used for pretreating BSS. It was found that combination pretreatment could effectively remove xylan and lignin for enhancing enzymatic saccharification. The reducing sugars and glucose from the hydrolysis of 100 g/L pretreated BSS with complexed cellulases of Galactomyces sp. CCZU11-1 were obtained in the yields of 81...
June 22, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28634846/lipid-accumulation-and-metabolic-analysis-based-on-transcriptome-sequencing-of-filamentous-oleaginous-microalgae-tribonema-minus-at-different-growth-phases
#3
Hui Wang, Lili Gao, Huimin Shao, Wenjun Zhou, Tianzhong Liu
Filamentous oleaginous microalgae specie Tribonema minus is a promising feedstock for biodiesel production. However, the metabolic mechanism of lipid production in this filamentous microalgal specie remains unclear. Here, we compared the lipid accumulation of T. minus at different growth phases, and described the de novo transcriptome sequencing and assembly and identified important pathways and genes involved in TAG production. Total lipid increased by 2.5-fold and its TAG level in total lipid reached 81.1% at stationary phase...
June 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28624929/latex-peptidases-of-calotropis-procera-for-dehairing-of-leather-as-an-alternative-to-environmentally-toxic-sodium-sulfide-treatment
#4
Laura M I Lopéz, Carolina A Viana, María E Errasti, María L Garro, José E Martegani, Germán A Mazzilli, Cléverson D T Freitas, Ídila M S Araújo, Rafaela O da Silva, Márcio V Ramos
Dehairing of crude leather is a critical stage performed at the beginning of its processing to obtain industrially useful pieces. Tanneries traditionally apply a chemical process based on sodium sulfide. Since this chemical reactive is environmentally toxic and inefficiently recycled, innovative protocols for reducing or eliminating its use in leather depilation are welcomed. Therefore, latex peptidases from Calotropis procera (CpLP) and Cryptostegia grandiflora (CgLP) were assayed for this purpose. Enzyme activity on substrates representative of skin such as hide powder azure (UHPA), elastin (UE), azocollagen (UAZOCOL), keratin (UK), and epidermis (UEP) was determined, while depilation activity was assayed on cow hide...
June 17, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28616686/mechanistic-approach-for-fabrication-of-gold-nanoparticles-by-nitzschia%C3%A2-diatom-and-their%C3%A2-antibacterial-activity
#5
Hemant P Borase, Chandrashekhar D Patil, Rahul K Suryawanshi, Sunil H Koli, Bhavana V Mohite, Giovanni Benelli, Satish V Patil
The problem of chemically synthesized nanoproducts motivated scientific community to explore ecofriendly methods of nanosynthesis. Diatoms belong to a group of aquatic, unicellular, photosynthetic microalgae have been scarcely investigated as a source of reducing and capping agents for nanosynthesis of pesticides and antibiotics. The present study reports a novel ecofriendly method for the fabrication of bioactive gold nanoparticles using locally isolated Nitzschia diatoms. The diatom-fabricated gold nanoparticles show characteristic ruby red colored with sharp absorbance peak at 529 nm...
June 14, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28612166/biodegradation-of-pyrene-by-candida-sp-s1-under-high-salinity-conditions
#6
Tony Hadibarata, Ameer Badr Khudhair, Risky Ayu Kristanti, Hesam Kamyab
Polycyclic aromatic hydrocarbon is a toxic recalcitrant environmental pollutant and its removal from the environment is very essential. In this study, a novel S1 strain isolated from the tropical rain forest was identified as Candida species based on 18S rRNA. The pyrene biodegradation was performed by Candida sp. S1. Pyrene was 35% degraded in 15 days. The percentage of pyrene biodegradation increased up to 75% with 24 g L(-1) of sodium chloride and decreased along with increasing salinity. Under the acidic condition, the biodegradation was increased up to 60% at pH 5...
June 13, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28601941/chitosan-based-hybrid-immobilization-in-bienzymatic-reactions-and-its-application-to-the-production-of-laminaribiose
#7
Clarissa Müller, Dave Hartig, Karl Vorländer, Ann-Cathérine Sass, Stephan Scholl, Hans-Joachim Jördening
A hybrid-immobilization method was developed to improve the long-term stability of laminaribiose phosphorylase immobilized on epoxy supports Sepabeads EC-EP/S. Entrapment in chitosan retained all of the enzyme activity depending on the amount of entrapped solid materials and increased half-life by a factor of 10-94.4 h. No enzyme activity loss was determined during 12 times reuse. The immobilization method is also applicable to sucrose phosphorylase immobilized on Sepabeads EC-EP/S. Up to 31.9 g/L laminaribiose were produced in bienzymatic batch experiments with reaction-integrated product separation by adsorption on zeolites...
June 10, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28597212/a-purely-green-synthesis-of-silver-nanoparticles-using-carica-papaya-manihot-esculenta-and-morinda-citrifolia-synthesis-and-antibacterial-evaluations
#8
Achmad Syafiuddin, Salmiati, Tony Hadibarata, Mohd Razman Salim, Ahmad Beng Hong Kueh, Ajeng Arum Sari
Green procedure for synthesizing silver nanoparticles (AgNPs) is currently considered due to its economy and toxic-free effects. Several existing works on synthesizing AgNPs using leaves extract still involve the use of physical or mechanical treatment such as heating or stirring, which consume a lot of energy. To extend and explore the green extraction philosophy, we report here the synthesis and antibacterial evaluations of a purely green procedure to synthesize AgNPs using Carica papaya, Manihot esculenta, and Morinda citrifolia leaves extract without the aforementioned additional treatment...
June 8, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28593458/sensitivity-based-adaptive-mesh-refinement-collocation-method-for-dynamic-optimization-of-chemical-and-biochemical-processes
#9
Long Xiao, Ping Liu, Xinggao Liu, Zeyin Zhang, Yalin Wang, Chunhua Yang, Weihua Gui, Xu Chen, Bochao Zhu
Collocation on finite element (CFE) is an effective simultaneous method of dynamic optimization to increase the profitability or productivity of industrial process. The approach needs to select an optimal mesh of time interval to balance the computational cost with desired solution. A new CFE approach with non-uniform refinement procedure based on the sensitivity analysis for dynamic optimization problems is, therefore, proposed, where a subinterval is further refined if the obtained control parameters have significant effect on the performance index...
June 7, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28593457/use-of-solvent-mixtures-for-total-lipid-extraction-of-chlorella-vulgaris-and-gas-chromatography-fame-analysis
#10
Narges Moradi-Kheibari, Hossein Ahmadzadeh, Majid Hosseini
Lipid extraction is the bottleneck step for algae-based biodiesel production. Herein, 12 solvent mixture systems (mixtures of three non-polar and two polar organic solvents) were examined to evaluate their effects on the total lipid yield from Chlorella vulgaris (C. vulgaris). Moreover, the extraction yields of three solvent systems with maximum extraction efficiency of esterifiable lipids were determined by acidic transesterification and GC-FID analysis. Three solvent systems, which resulted in a higher extraction yield, were further subjected to fatty acid methyl ester (FAME) analysis...
June 7, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28589216/mathematical-modeling-of-the-ethanol-fermentation-of-cashew-apple-juice-by-a-flocculent-yeast-the-effect-of-initial-substrate-concentration-and-temperature
#11
Álvaro Daniel Teles Pinheiro, Andréa da Silva Pereira, Emanuel Meneses Barros, Sandra Regina Ceccato Antonini, Samuel Jorge Marques Cartaxo, Maria Valderez Ponte Rocha, Luciana Rocha B Gonçalves
In this work, the effect of initial sugar concentration and temperature on the production of ethanol by Saccharomyces cerevisiae CCA008, a flocculent yeast, using cashew apple juice in a 1L-bioreactor was studied. The experimental results were used to develop a kinetic model relating biomass, ethanol production and total reducing sugar consumption. Monod, Andrews, Levenspiel and Ghose and Tyagi models were investigated to represent the specific growth rate without inhibition, with inhibition by substrate and with inhibition by product, respectively...
June 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28585068/a-novel-nitrilase-from-ralstonia-eutropha-h16-and-its-application-to-nicotinic-acid-production
#12
Haiyang Fan, Lifeng Chen, Huihui Sun, Hualei Wang, Yuhong Ren, Dongzhi Wei
A novel aliphatic nitrilase, REH16, was found in Ralstonia eutropha H16 and overexpressed in Escherichia coli BL21(DE3), and its enzymatic properties were studied. The temperature and pH optima were 37 °C and 6.6, respectively, and the best thermostability of the nitrilase was observed at 25 °C, which preserved 95% of activity after 120 h of incubation. REH16 has a broad hydrolytic activity toward aliphatic and heterocyclic nitriles and showed high tolerance of 3-cyanopyridine; this enzyme could hydrolyze as high as 100 mM 3-cyanopyridine completely...
June 5, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28577048/impact-of-pluronic-%C3%A2-f68-on-hollow-fiber-filter-based-perfusion-culture-performance
#13
Sen Xu, Rubin Jiang, Yuetian Chen, Fengqiang Wang, Hao Chen
High cell density is an important factor in achieving high bioreactor productivity. To meet the oxygen demand with density at >100 × 10(6) cells/mL, a frit sparger is often used. In this study, the impact of Pluronic(®) F68 on a perfusion process using a frit sparger was studied. The perfusion process was developed using an alternating tangential flow device with a 0.2 µm PES hollow fiber filter. Pluronic(®) F68 at 2 g/L was sufficient in preventing cell damage at gas flow rate of ~0.20 vvm from a drilled hole sparger (0...
June 2, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28567527/-13-c-assisted-metabolomics-analysis-reveals-the-positive-correlation-between-specific-erythromycin-production-rate-and-intracellular-propionyl-coa-pool-size-in-saccharopolyspora-erythraea
#14
Ming Hong, Han Mou, Xiaoyun Liu, Mingzhi Huang, Ju Chu
Metabolomics analysis is extremely essential to explore the metabolism characteristics of Saccharopolyspora erythraea. The lack of suitable methods for the determination of intracellular metabolites, however, hinders the application of metabolomics analysis for S. erythraea. Acyl-CoAs are important precursors of erythromycin; phosphorylated sugars are intermediate metabolites in EMP pathway or PPP pathway; organic acids are intermediate metabolites in TCA cycle. Reliable determination methods for intracellular acyl-CoAs, phosphorylated sugars, and organic acids of S...
May 31, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28567526/mathematical-modelling-and-kinetic-study-for-cd-production-catalysed-by-toruzyme-%C3%A2-and-cgtase-from-bacillus-firmus-strain-37
#15
Keren Hapuque Pinheiro, Leonardo Brunelli do Nascimento, Vanderson Carvalho Fenelon, Carlos Eduardo Barão, Graciette Matioli, Flavio Faria de Moraes
A new mathematical model was developed for the kinetics of α-, β- and γ-cyclodextrin production, expanding an existing model that only included the production of β- and γ-cyclodextrins, because a detailed kinetic modelling of the reactions involved allows the manipulation of the process yields. The kinetic behaviour of the commercial enzyme Toruzyme(®) was studied with maltodextrin as substrate at different concentrations and for CGTase from Bacillus firmus strain 37 at a concentration of 100 g L(-1)...
May 31, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28551857/efficient-enzymatic-degradation-used-as-pre-stage-treatment-for-norfloxacin-removal-by-activated-sludge
#16
Ruinan Zhao, Xiaohong Li, Mancheng Hu, Shuni Li, Quanguo Zhai, Yucheng Jiang
Norfloxacin is often found in wastewater treatment plants, groundwater, and even drinking water causing environmental concerns because of its potential undesirable effects on human health or aquatic ecosystems. However, conventional treatments cannot deal with norfloxacin efficiently. This work proposes an efficiently enzymatic degradation of norfloxacin by chloroperoxidase (CPO). 82.18% degradation efficiency of norfloxacin was achieved after 25 min reaction time at pH 5.0 with an enzyme concentration of 1...
May 27, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28551856/model-based-intensification-of-a-fed-batch-microbial-process-for-the-maximization-of-polyhydroxybutyrate-phb-production-rate
#17
Giannis Penloglou, Athina Vasileiadou, Christos Chatzidoukas, Costas Kiparissides
An integrated metabolic-polymerization-macroscopic model, describing the microbial production of polyhydroxybutyrate (PHB) in Azohydromonas lata bacteria, was developed and validated using a comprehensive series of experimental measurements. The model accounted for biomass growth, biopolymer accumulation, carbon and nitrogen sources utilization, oxygen mass transfer and uptake rates and average molecular weights of the accumulated PHB, produced under batch and fed-batch cultivation conditions. Model predictions were in excellent agreement with experimental measurements...
May 27, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28547539/cultivation-of-scenedesmus-dimorphus-using-anaerobic-digestate-as-a-nutrient-medium
#18
Husam A Abu Hajar, R Guy Riefler, Ben J Stuart
In this study, the microalga Scenedesmus dimorphus was cultivated phototrophically using unsterilized anaerobic digestate as a nutrient medium. A bench-scale experiment was conducted by inoculating the microalga S. dimorphus with 0.05-10% dilutions of the anaerobic digestate supernatant. It was found that 1.25-2.5% dilutions, which is equivalent to 50-100 mg N/L total nitrogen concentrations and 6-12 mg P/L total phosphorus concentrations, provided sufficient nutrients to maximize the growth rate along with achieving high concentrations of algal biomass...
May 25, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28540439/characterization-of-silver-nanoparticles-produced-by-biosynthesis-mediated-by-fusarium-oxysporum-under-different-processing-conditions
#19
Édipo S Almeida, Débora de Oliveira, Dachamir Hotza
The goal of this study was the biosynthesis of silver nanoparticles (SNPs) mediated by the fungus Fusarium oxysporum, as well as the characterization of these nanoparticles including evaluation of the particles size and stability under different processing conditions. The results showed that the biosynthesis produced silver nanoparticles having a mean size of 34 nm and zeta potential values below -30 mV at the conditions used, characterizing the nanoparticles as being stable in suspension. Ultraviolet-visible spectroscopy and flame atomic absorption spectroscopy confirmed the formation of silver nanoparticles and Fourier transform infrared spectroscopy detected the bands corresponding to the binding vibration of amide I and II bands of proteins in addition to the presence of cyclic alkanes, cyclohexane, ethers, and aromatic hydrocarbons...
May 24, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28536853/simultaneous-production-of-laccase-and-degradation-of-bisphenol-a-with-trametes-versicolor-cultivated-on-agricultural-wastes
#20
Shengquan Zeng, Jie Zhao, Liming Xia
Solid state fermentation with Trametes versicolor was carried out on agricultural wastes containing bisphenol A (BPA). It was found that BPA degradation was along with the occurrence of laccase production, and wheat bran and corn straw were identified as suitable mixed substrates for laccase production. In the process of BPA degradation with T. versicolor, laccase activity increased rapidly at the 6th-10th day after inoculation. Moreover, BPA can enhance the production of laccase. After 10 days of fermentation, degradation rate of BPA exceeded 90% without the usage of mediators ABTS and acetosyringone at pH 4...
May 23, 2017: Bioprocess and Biosystems Engineering
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