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FEMS Yeast Research

Karen L Hinkle, Darlene Olsen
The pesticide 3-trifluoromethyl-4-nitrophenol (TFM) is used to control sea lamprey populations in the Great Lakes and Lake Champlain. Little is known about the effects of this pesticide on gene expression in eukaryotic species. This study used microarray analysis to determine the effects of short term, high dose TFM exposure on gene expression in Saccharomyces cerevisiae. Yeast grown in standard glucose-containing media showed significant variation in gene expression in pathways related to cytoplasmic translation with the majority of these genes being downregulated...
November 15, 2018: FEMS Yeast Research
Jennifer E G Gallagher
Ribosomes are synthesized by large ribonucleoprotein complexes cleaving and properly assembling highly structured rRNAs with ribosomal proteins. Transcription and processing of pre-rRNAs are linked by the t-Utps, a sub-complex of the SSU (small subunit) processome and prompted the investigations for the requirements of t-Utp formation and transition into the SSU processome. The rDNA promoter, the first 44 nucleotides of the 5'ETS, and active transcription by pol I were sufficient to recruit the t-Utps to the rDNA...
November 15, 2018: FEMS Yeast Research
Ana Claudia G Zimbres, Julia C V Reuwsaat, Vanessa A Barcellos, Luna S Joffe, Fernanda L Fonseca, Charley C Staats, Augusto Schrank, Livia Kmetzsch, Marilene H Vainstein, Marcio L Rodrigues
Melanin formation is a promising target for antifungal development. We screened a collection of 727 compounds that were previously approved for clinical use in humans for inhibition of pigmentation in Cryptococcus gattii, a lethal fungal pathogen that causes damage to both immunocompetent and immunocompromised hosts. The pyrimidine analogues flucytosine (5-fluorocytosine; 5-FC), 5- fluorouracil (5-FU) and carmofur were identified as efficient inhibitors of pigmentation in the C. gattii model. Since melanin synthesis is enzymatically catalyzed by laccase in Cryptococcus, we investigated whether inhibition of pigmentation by the pyrimidine analogues was laccase-mediated...
November 10, 2018: FEMS Yeast Research
Deepak Kumar, Nimisha Sharma
Transcription of protein coding genes is a highly regulated process. In eukaryotes, it involves cross talk between the RNA polymerase II enzyme and different proteins of the transcriptional machinery. Twelve different subunits, Rpb1 to Rpb12, constitute RNA polymerase II. The sequence of the Rpb7 subunit is highly conserved across organisms. However, our knowledge and understanding of the role of Rpb7 in Schizosaccharomyces pombe is still limited. Therefore, in the present study we have studied the transcriptional response of Schizosaccharomyces pombe cells expressing reduced levels of rpb7+...
November 5, 2018: FEMS Yeast Research
Steffi A Davison, Riaan den Haan, Willem Heber van Zyl
The yeast Saccharomyces cerevisiae is considered an important host for consolidated bioprocessing and the production of high titres of recombinant cellulases are required for efficient hydrolysis of lignocellulosic substrates to fermentable sugars. Since recombinant protein secretion profiles vary highly among different strain backgrounds, careful selection of robust strains with optimal secretion profiles are of crucial importance. Here, we construct and screen sets of haploid derivatives, isolated from natural strain isolates YI13, FINI and YI59, for improved general cellulase secretion...
November 1, 2018: FEMS Yeast Research
R N de Witt, H Kroukamp, H Volschenk
Strains of Saccharomyces cerevisiae with improved tolerance to plant hydrolysates are of utmost importance for the cost-competitive production of value-added chemicals and fuels. However, engineering strategies are constrained by a lack of understanding of the yeast response to complex inhibitor mixtures. Natural S. cerevisiae isolates display niche-specific phenotypic and metabolic diversity, encoded in their DNA, which has evolved to overcome external stresses, utilise available resources and ultimately thrive in their challenging environments...
October 27, 2018: FEMS Yeast Research
Rui Silva, Tatiana Q Aguiar, Rui Oliveira, Lucília Domingues
The overproduction of riboflavin (vitamin B2) by Ashbya gossypii, one of the most distinctive traits of this filamentous hemiascomycete, has been proposed to act as an ecological defense mechanism, since it is triggered by environmental stress. The interaction of endogenous riboflavin with light generates reactive oxygen species (ROS) and induces oxidative DNA damage in mammalian cells, but exogenous riboflavin was shown to protect A. gossypii spores against UV light. Envisioning a better understanding of this biotechnologically relevant trait, here we investigated the putative genotoxic effects associated with the overproduction of riboflavin by A...
October 13, 2018: FEMS Yeast Research
Rebecca C Deed, Ruoyu Hou, Matias I Kinzurik, Richard C Gardner, Bruno Fedrizzi
3-(Methylthio)-1-propanol (methionol), produced by yeast as an end-product of L-methionine (L-Met) catabolism, imparts off-odours reminiscent of cauliflower and potato to wine. Saccharomyces cerevisiae ARO genes, including transaminases Aro8p and Aro9p, and decarboxylase Aro10p, catalyse two key steps forming methionol via the Ehrlich pathway. We compared methionol concentrations in wines fermented by single Δaro8, Δaro9, and Δaro10 deletants in lab strain BY4743 versus wine strain Zymaflore F15, and F15 double- and triple-aro deletants versus single-aro deletants, using headspace-solid phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME/GC-MS)...
October 1, 2018: FEMS Yeast Research
Pornkamol Unrean, Jochem Gätgens, Bianca Klein, Stephan Noack, Verawat Champreda
A robust cell factory that can tolerate combined inhibitory lignocellulosic compounds is essential for the cost-effective lignocellulose-based production of second-generation bioethanol and other bulk chemicals. Following high-throughput phenotyping of a yeast genomic overexpression library, we identified a Saccharomyces cerevisiae mutant (denoted AFb.01) with improved growth and fermentation performance under combined toxicity of acetic acid and furfural. AFb.01 carries overexpression of TRX1, which encodes for thioredoxin, a cellular redox machinery...
September 25, 2018: FEMS Yeast Research
Jasmine M Bracher, Oscar A Martinez-Rodriguez, Wijb J C Dekker, Maarten D Verhoeven, Antonius J A van Maris, Jack T Pronk
Expression of a heterologous xylose isomerase, deletion of the GRE3 aldose-reductase gene and overexpression of genes encoding xylulokinase (XKS1) and non-oxidative pentose-phosphate-pathway enzymes (RKI1, RPE1, TAL1, TKL1) enables aerobic growth of Saccharomyces cerevisiae on d-xylose. However, literature reports differ on whether anaerobic growth on d-xylose requires additional mutations. Here, CRISPR-Cas9-assisted reconstruction and physiological analysis confirmed an early report that this basic set of genetic modifications suffices to enable anaerobic growth on d-xylose in the CEN...
September 24, 2018: FEMS Yeast Research
Miguel Fernández-Niño, Sergio Pulido, Despina Stefanoska, Camilo Pérez, Daniel González-Ramos, Antonius J A van Maris, Kathleen Marchal, Elke Nevoigt, Steve Swinnen
Acetic acid tolerance of the yeast Saccharomyces cerevisiae is manifested in several quantifiable parameters, of which the duration of the latency phase is one of the most studied. It has been shown recently that the latter parameter is mostly determined by a fraction of cells within the population that resumes proliferation upon exposure to acetic acid. The aim of the current study was to identify genetic determinants of the difference in this parameter between the highly tolerant strain MUCL 11987-9 and the laboratory strain CEN...
September 14, 2018: FEMS Yeast Research
Susana Perrusquía-Luévano, Marlene Sarahí Cano-Herrera, César Guigón-López, María Del Carmen Avitia-Talamantes, Carlos Torres-Torres, Isaela Villalpando
This work reports important results of aromatic profiles produced by native yeasts isolated from desert-grown wine grapes from the south of Chihuahua, México. These grapes stand very high temperatures during the ripening season, developing high sugar concentration and high pH. Yeast species found in grapes were identified by polymerase chain reaction and sequence analysis of the 5.8S internal transcribed spacer ribosomal RNA region. Aureobasidium namibiae, Sporobolomyces johnsonii, Candida apicola, Hanseniaspora uvarum, Candida thaimueangensis, Hanseniaspora opuntiae, were identified...
September 10, 2018: FEMS Yeast Research
Michael Gossing, Agata Smialowska, Jens Nielsen
Nutrient sensing and signaling controls the cellular response to extracellular nutrients and intracellular metabolites. Nutrient-dependent regulation of metabolism ensures balanced energy production and expenditure. We show that disturbing energy balance by forcing fatty acid synthesis has profound impact on metabolism and physiology of the yeast cell. In addition to an expected increase in storage lipids, we observed increased β-oxidation and reduced amino acid biosynthesis, indicating increased activity of nutrient-sensitive kinase Snf1p...
August 29, 2018: FEMS Yeast Research
Brooke J Bevis
No abstract text is available yet for this article.
January 1, 2019: FEMS Yeast Research
Reed B Wickner
I retrace my path from math to medicine to biochemistry to yeast genetics, my focus on infectious diseases of yeast and finally prions. My discovery of yeast prions relied on my particular focus on the logical relations of non-chromosomal genetic elements and the chromosomal genes involved in their propagation and expression. Pursuing an understanding of yeast prions involved structural biology based on genetics, solid-state NMR, population genetics and more genetics.
January 1, 2019: FEMS Yeast Research
Sudharshan Sj, Bhavana Veerabhadrappa, Subasri Subramaniyan, Madhu Dyavaiah
The budding yeast, Saccharomyces cerevisiae, is an efficient model for studying oxidative stress, programmed cell death and aging. The present study was carried out to investigate antioxidant, the anti-apoptotic and anti-aging activity of a natural compound, astaxanthin, in S. cerevisiae model. The survivability of yeast antioxidant-deficient strains (sod1Δ, sod2Δ, cta1Δ, ctt1Δ and tsa1Δ) increased by 20%-40% when cells were pre-treated with astaxanthin, compared to hydrogen peroxide alone, as demonstrated in spot and colony forming unit assays...
January 1, 2019: FEMS Yeast Research
Eliška Krocová, Sylva Neradová, Rudolf Kupcik, Sylva Janovská, Zuzana Bílková, Olga Heidingsfeld
Most of the phosphatases of human fungal pathogens Candida albicans and C. parapsilosis have never been experimentally characterised, although dephosphorylation reactions are central to many biological processes. PHO15 genes of these yeasts have been annotated as the sequences encoding 4-nitrophenyl phosphatase, on the basis of homology to PHO13 gene from the bakers' yeast Saccharomyces cerevisiae. To examine the real function of these potential phosphatases from Candida spp., CaPho15p and CpPho15p were prepared using expression in Escherichia coli and characterised...
January 1, 2019: FEMS Yeast Research
P Pracharova, P Lieben, B Pollet, J M Beckerich, P Bonnarme, S Landaud, D Swennen
Geotrichum candidum is a fungus-like yeast widely used as a starter culture for cheese ripening for its proteolytic and lipolytic activities and its contribution to the cheese flavours. The sequenced strain G. candidum CLIB 918 was isolated from cheese Pont-L'Evêque. This strain's ability to produce volatile compounds was compared to the ability of a known strong sulphur compound producer G. candidum strain (Gc203). The aminotransferase-coding genes BAT2 and ARO8 were identified to be involved in methionine catabolism...
January 1, 2019: FEMS Yeast Research
Melanie Wijsman, Michal A Swiat, Wesley L Marques, Johanna K Hettinga, Marcel van den Broek, Pilar de la Torre Cortés, Robert Mans, Jack T Pronk, Jean-Marc Daran, Pascale Daran-Lapujade
Hexose transporter-deficient yeast strains are valuable testbeds for the study of sugar transport by native and heterologous transporters. In the popular Saccharomyces cerevisiae strain EBY.VW4000, deletion of 21 transporters completely abolished hexose transport. However, repeated use of the LoxP/Cre system in successive deletion rounds also resulted in major chromosomal rearrangements, gene loss and phenotypic changes. In the present study, CRISPR/SpCas9 was used to delete the 21 hexose transporters in an S...
January 1, 2019: FEMS Yeast Research
Young-Kyoung Park, Paulina Korpys, Monika Kubiak, Ewelina Celinska, Paul Soudier, Pauline Trébulle, Macarena Larroude, Tristan Rossignol, Jean-Marc Nicaud
The non-conventional model yeast Yarrowia lipolytica is of increasing interest as a cell factory for producing recombinant proteins or biomolecules with biotechnological or pharmaceutical applications. To further develop the yeast's efficiency and construct inducible promoters, it is crucial to better understand and engineer promoter architecture. Four conserved cis-regulatory modules (CRMs) were identified via phylogenetic footprinting within the promoter regions of EYD1 and EYK1, two genes that have recently been shown to be involved in erythritol catabolism...
January 1, 2019: FEMS Yeast Research
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