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FEMS Yeast Research

Heejeong Lee, Eun-Rhan Woo, Dong Gun Lee
Apigenin, a natural flavone, has been well characterized for its their anticarcinogenic property; however, its bioactivity against pathogenic fungi has not been investigated in detail. In the present study, we examined the antifungal activity and mode of action of apigenin. Apigenin inhibited the growth of fungal pathogens, which induced superficial infection and reduced biofilm mass. Three-dimensional flow cytometric analysis demonstrated that apigenin induced morphological changes, especially cell shrinkage, in Candida albicans...
January 16, 2018: FEMS Yeast Research
Ming Zhang, Wenqiang Chang, Hongzhuo Shi, Ying Li, Sha Zheng, Wei Li, Hongxiang Lou
Candida albicans, one most prevalent fungal pathogen, causes severe mucosal and invasive infections in predisposed individuals. The rise of fungal infection and drug-resistance demands the development of novel antifungal agents. In this study, we observed that floricolin C (FC), a p-terphenyl pigment from an endolichenic fungus, killed C. albicans cells at planktonic state or within biofilms through reactive oxygen species (ROS) accumulation. Further test revealed that FC could directly damage the mitochondria to cause ROS accumulation...
January 15, 2018: FEMS Yeast Research
In Iok Kong, Timothy Lee Turner, Heejin Kim, Soo Rin Kim, Yong-Su Jin
Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeast, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically-modified S. cerevisiae have been found in industrial environments for various purposes. These industrial strains are isolated from industrial fermentation sites and they are considered as potential host strains for superior fermentation processes...
January 9, 2018: FEMS Yeast Research
Karen O Osiro, Daniel P Brink, Celina Borgström, Lisa Wasserstrom, Magnus Carlquist, Marie F Gorwa-Grauslund
One of the challenges of establishing an industrially competitive process to ferment lignocellulose to value-added products using Saccharomyces cerevisiae is to get efficient mixed sugar fermentations. Despite successful metabolic engineering strategies, the xylose assimilation rates of recombinant S. cerevisiae remain significantly lower than for the preferred carbon source, glucose. Previously we established a panel of in vivo biosensor strains (TMB371X) where different promoters (HXT1/2/4p; SUC2p, CAT8p; TPS1p/2p, TEF4p) from the main sugar signaling pathways were coupled with the yEGFP3 gene, and observed that wild type S...
January 5, 2018: FEMS Yeast Research
Yasuyuki Suda, Hiroyuki Tachikawa, Ichiro Inoue, Tomokazu Kurita, Chieko Saito, Kazuo Kurokawa, Akihiko Nakano, Kenji Irie
Sec2 activates Sec4 Rab GTPase as a gunanine nucleotide exchange factor for the recruitment of downstream effectors to facilitate tethering and fusion of post-Golgi vesicles at the plasma membrane. During the meiosis and sporulation of budding yeast, post-Golgi vesicles are transported to and fused at the spindle pole body (SPB) to form a de novo membrane, called the prospore membrane. Previous studies have revealed the role of the SPB outer surface called the meiotic outer plaque (MOP) in docking and fusion of post-Golgi vesicles...
December 27, 2017: FEMS Yeast Research
Gesine Hoffmann, Anke Samel-Pommerencke, Jan Weber, Alessandro Cuomo, Tiziana Bonaldi, Ann E Ehrenhofer-Murray
Centromeres are the sites of assembly of the kinetochore, which connect the chromatids to the microtubules for sister chromatid segregation during cell division. Centromeres are characterized by the presence of the histone H3 variant CENP-A (termed Cse4 in Saccharomyces cerevisiae). Here, we investigated the function of serine 33 phosphorylation of Cse4 (Cse4-S33ph) in S. cerevisiae, which lies within the essential N-terminal domain (END) of the extended Cse4 N-terminus. Significantly, we identified histone H4-K5, 8, 12R to cause a temperature-sensitive growth defect with mutations in Cse4-S33 and sensitivity to nocodazole and hydroxyurea...
December 20, 2017: FEMS Yeast Research
F A Sabirzyanov, T A Sabirzyanova, V V Rekstina, A A Adzhubei, T S Kalebina
A cell wall (CW) provides a protective barrier for a yeast cell and is a firm structure that nevertheless dynamically changes during cell's growth. Bgl2p is a non-covalently anchored glucanosyltransglycosylase in the CW of the yeast Saccharomyces cerevisiae. The mode of its anchorage is poorly understood, while its association with CW components is tight and resistant to one-hour treatment with 1% SDS at 37°C. In order to demarcate the potential structural block responsible for incorporation of Bgl2p into the CW, bioinformatics analysis of its sequence was performed, and a conservative structural region was identified in the C-terminal region of Bgl2p, that was absent in its homologues in S...
December 20, 2017: FEMS Yeast Research
C Grangeteau, V David, H Alexandre, M Guilloux-Benatier, S Rousseaux
Although differences in yeast biodiversity have often been found between vineyards subjected to organic protection or conventional protection, little is known about the effect of copper and sulfur fungicides (the only fungicides allowed in organic farming) on yeast populations. The sensitivity to copper and sulfur of 158 yeast isolates of seven different species (Aureobasidium pullulans, Hanseniaspora guilliermondii, Hanseniaspora uvarum, Metschnikowia sp., Pichia membranifaciens, Saccharomyces cerevisiae and Starmerella bacillaris) was evaluated...
November 29, 2017: FEMS Yeast Research
Manuela Gottardi, Peter Grün, Helge B Bode, Thomas Hoffmann, Wilfried Schwab, Mislav Oreb, Eckhard Boles
Trans-cinnamic acid (tCA) and hydrocinnamyl alcohol (HcinOH) are valuable aromatic compounds with applications in the flavour, fragrance and cosmetic industry. They can be produced with recombinant yeasts from sugars via phenylalanine after expression of a phenylalanine ammonia lyase (PAL) and an aryl carboxylic acid reductase (ACAR). Here we show that in Saccharomyces cerevisiae a PAL enzyme from the bacterium Photorhabdus luminescens was superior to a previously used plant PAL enzyme for the production of tCA...
November 23, 2017: FEMS Yeast Research
Valter Longo
No abstract text is available yet for this article.
November 23, 2017: FEMS Yeast Research
Kashif Rahim, Liang Huo, Chenxi Li, Ping Zhang, Abdul Basit, Biyun Xiang, Bie Ting, Xiaoran Hao, Xudong Zhu
Cryptococcus neoformans is a basidiomycetous pathogenic yeast that causes fatal infections in both immunocompetent and immunocompromised patients. Regulation on the production of its virulence factor is not fully understood. Here we reported the characterization of a gene, named CVH1(CNA06260), encoding a Drosophila Vilse-like RhoGAP homologue, which is hallmarked by three conserved functional domains, i.e. WW, MyTH4, and RhoGAP. Phylogenetic analysis suggests that CVH1 is highly conserved from protists to mammals and interestingly in basidiomycetes, but absent in plants or Ascomycota and other lower fungi...
November 21, 2017: FEMS Yeast Research
Diogo Dias Castanheira, Eduardo Perovano Santana, Fernanda Godoy-Santos, Raphael Hermano Santos Diniz, Fábio Faria-Oliveira, Renata Rebeca Pereira, Maria José Magalhães Trópia, Ieso Miranda Castro, Rogelio Lopes Brandão
In yeast, as in other eukaryotes, calcium plays an essential role in signaling transduction to regulate different processes. Many pieces of evidence suggest that glucose-induced activation of plasma membrane H+-ATPase, essential for yeast physiology, is related to calcium signaling. Until now, it was not identified any protein that could be regulated by calcium in this context. Lpx1p, a serine-protease that is also involved in the glucose-induced activation of the plasma membrane H+-ATPase activation, could be a candidate to respond to intracellular calcium signaling involved in this process...
November 20, 2017: FEMS Yeast Research
Christina Smolke
No abstract text is available yet for this article.
November 17, 2017: FEMS Yeast Research
Roman Maslanka, Magdalena Kwolek-Mirek, Renata Zadrag-Tecza
Glucose plays an important role in cell metabolism and has an impact on cellular physiology. Changes in glucose availability may strongly influence growth rate of the cell size, cell metabolism and the rate of generation of cellular by-products, such as reactive oxygen species. The positive effect of low glucose concentration conditions-calorie restriction is observed in a wide range of species, including the Saccharomyces cerevisiae yeast, yet little is known about the effect of high glucose concentrations-calorie excess...
November 14, 2017: FEMS Yeast Research
Joanna Rytka
No abstract text is available yet for this article.
November 14, 2017: FEMS Yeast Research
Robert Mans, Else-Jasmijn Hassing, Melanie Wijsman, Annabel Giezekamp, Jack T Pronk, Jean-Marc Daran, Antonius J A van Maris
CRISPR/Cas9-based genome editing allows rapid, simultaneous modification of multiple genetic loci in Saccharomyces cerevisiae. Here, this technique was used in a functional analysis study aimed at identifying the hitherto unknown mechanism of lactate export in this yeast. First, an S. cerevisiae strain was constructed with deletions in 25 genes encoding transport proteins, including the complete aqua(glycero)porin family and all known carboxylic-acid transporters. The 25-deletion strain was then transformed with an expression cassette for Lactobacillus casei lactate dehydrogenase (LcLDH)...
November 14, 2017: FEMS Yeast Research
Akaraphol Watcharawipas, Daisuke Watanabe, Hiroshi Takagi
Sodium and acetate inhibit cell growth and ethanol fermentation by different mechanisms in Saccharomyces cerevisiae. We identified the substitution of a conserved Thr255 to Ala (T255A) in the essential Nedd4-family ubiquitin ligase Rsp5, which enhances cellular sodium acetate tolerance. The T255A mutation selectively increased the resistance of cells against sodium acetate, suggesting that S. cerevisiae cells possess an Rsp5-mediated mechanism to cope with the composite stress of sodium and acetate. The sodium acetate tolerance was dependent on the extrusion of intracellular sodium ions by the plasma membrane-localized sodium pumps Ena1, Ena2, and Ena5 (Ena1/2/5) and two known upstream regulators: the Rim101 pH signaling pathway and the Hog1 mitogen-activated protein kinase (MAPK)...
November 2, 2017: FEMS Yeast Research
Ema Cavallo, Hernán Charreau, Patricia Cerrutti, María Laura Foresti
Every year more than 2 million tons of citric acid (CA) are produced around the world for industrial uses. Although initially extracted from citrus, the low profitability of the process and the increasing demand soon stimulated the search for more efficient methods to produce CA. Currently, most world CA demand (99%) is satisfied by fermentations with microorganisms, especially filamentous fungi and yeasts. CA production with yeasts has certain advantages over molds (e.g. higher productivity and easier cultivation), which in the last two decades have triggered a clear increase in publications and patents devoted to the use of yeasts in this field...
November 2, 2017: FEMS Yeast Research
Irina Borodina, Zongbao Kent Zhao
No abstract text is available yet for this article.
November 1, 2017: FEMS Yeast Research
Yueping Zhang, Jens Nielsen, Zihe Liu
Terpenoids represent a large class of natural products with significant commercial applications. These chemicals are currently mainly obtained through extraction from plants and microbes or through chemical synthesis. However, these sources often face challenges of unsustainability and low-productivity. In order to address these issues, Escherichia coli and yeast have been metabolic engineered to produce non-native terpenoids. With recent reports of engineering yeast metabolism to produce several terpenoids at high yields, it has become possible to establish commercial yeast production of terpenoids that find applications as perfume ingredients, pharmaceuticals, and advanced biofuels...
October 31, 2017: FEMS Yeast Research
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