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FEMS Yeast Research

Joshua A Kritzer, Yelena Freyzon, Susan Lindquist
Tyrosine phosphorylation is a key biochemical signal that controls growth and differentiation in multicellular organisms. Saccharomyces cerevisiae and nearly all other unicellular eukaryotes lack intact phosphotyrosine signaling pathways. However, many of these organisms have primitive phosphotyrosine-binding proteins and tyrosine phosphatases, leading to the assumption that the major barrier for emergence of phosphotyrosine signaling was the negative consequences of promiscuous tyrosine kinase activity. In this work, we reveal that the classic oncogene v-Src, which phosphorylates many dozens of proteins in yeast, is toxic because it disrupts a specific spore wall remodeling pathway...
March 13, 2018: FEMS Yeast Research
Patrícia Branco, Helena Albergaria, Nils Arneborg, Catarina Prista
Saccharomyces cerevisiae secretes antimicrobial peptides (AMPs) derived from glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which induce death of several non-Saccharomyces yeasts. Previously, we demonstrated that the naturally-secreted GAPDH-derived AMPs (i.e. saccharomycin) caused a loss of culturability and decreased the intracellular pH (pHi) of Hanseniaspora guilliermondii cells. In this study, we show that chemically-synthesized analogues of saccharomycin also induce a pHi drop and loss of culturability in H...
March 13, 2018: FEMS Yeast Research
Dana Szabóová, Ivan Hapala, Pavol Sulo
The complete mitochondrial DNA (mtDNA) sequence from Kazachstania sinensis was analyzed and compared to mtDNA from related yeasts. It contained the same set of genes; however, it only contained 23 tRNAs, as the trnR2 gene was absent. Most of the 12 introns within cox1, cob and rnl genes were inserted in the same sites as in other yeasts; however, two introns in rnl were in unusual positions. Traits such as gene order and GC cluster number were more related to Saccharomyces than to the other Kazachstania or linked clades...
March 8, 2018: FEMS Yeast Research
Noori Chai, Aaron D Gitler
A hexanucleotide repeat expansion in the C9orf72 gene has been identified as the most common cause of amyotrophic lateral sclerosis and frontotemporal dementia. The expanded hexanucleotide repeat is translated by an unconventional mechanism to produce five species of dipeptide repeat proteins (DPRs), glycine-proline (GP), glycine-alanine, glycine-arginine (GR), proline-alanine (PA), and proline-arginine (PR). Of these, the arginine-rich ones, PR and GR, are highly toxic in a variety of model systems, ranging from human cells, to Drosophila, to even the budding yeast, Saccharomyces cerevisiae...
March 8, 2018: FEMS Yeast Research
Tânia Raquel Rodrigues Grainha, Paula Alexandra da Silva Jorge, Martín Pérez-Pérez, Gael Pérez Rodríguez, Maria Olívia Baptista Oliveira Pereira, Anália Maria Garcia Lourenço
The complex virulence attributes of Candida albicans are an attractive target to exploit in the development of new antifungals and anti-virulence strategies to combat C. albicans infections. Particularly, quorum-sensing (QS) has been reported as critical for virulence regulation in C. albicans. This work presents two knowledge networks with up-to-date information about QS regulation and experimentally tested anti-QS and anti-virulence agents for C. albicans. A semi-automatic bioinformatics workflow that combines literature mining and expert curation was used to retrieve otherwise scattered information from the scientific literature...
March 6, 2018: FEMS Yeast Research
Tim Meier-Dörnberg, Oliver Ingo Kory, Fritz Jacob, Maximilian Michel, Mathias Hutzler
Saccharomyces cerevisiae variety diastaticus is generally considered to be an obligatory spoilage microorganism and spoilage yeast in beer and beer-mixed beverages (Folz, Hofmann and Stahl 2011; Hutzler et al. 2012). Their super-attenuating ability causes increased carbon dioxide concentrations, beer gushing and potential bottle explosion along with changes in flavor, sedimentation and increased turbidity. This research shows clear differences in the super-attenuating properties of S. cerevisiae var. diastaticus yeast strains and their potential for industrial brewing applications...
March 6, 2018: FEMS Yeast Research
Hajnalka Csoma, Lajos Ács-Szabó, László Attila Papp, Matthias Sipiczki
Starmerella bacillaris (Candida zemplinina) is a genetically heterogeneous species. In this work the diversity of 41 strains of various origins is examined and compared by the analysis of the length polymorphism of nuclear microsatellites and the RFLP of mitochondrial genomes. The band patterns are analysed with UPGMA, Neighbor Joining, Neighbor Net, Minimum Spanning Tree and Non-metric MDS algorithms. The results and their comparison to previous analyses demonstrate that different markers and different clustering methods can result in very different groupings of the same strains...
March 6, 2018: FEMS Yeast Research
Enea Gino Di Domenico, Ilaria Cavallo, Maria Guembe, Grazia Prignano, Maria Teresa Gallo, Valentina Bordignon, Giovanna D'Agosto, Isabella Sperduti, Luigi Toma, Fabrizio Ensoli
Candida species are opportunistic pathogens responsible for a variety of diseases, ranging from skin and mucosal lesions to severe systemic, life-threatening infections. Candida albicans accounts for more than 70% of all Candida infections, however, the clinical relevance of other species such as Candida parapsilosis and Candida krusei are being increasingly recognized. Biofilm-producing yeasts cells acquire an increased resistance to antifungal agents, often leading to therapeutic failure and chronic infection...
March 6, 2018: FEMS Yeast Research
Marianna Yurkiv, Olena Kurylenko, Roksolana Vasylyshyn, Kostyantyn Dmytruk, Patrick Fickers, Andriy Sibirny
Glutathione is the most abundant cellular thiol and the low molecular weight peptide present in cells. The methylotrophic yeast Ogataea (Hansenula) polymorpha is considered as a promising cell factory for the synthesis of glutathione. In this study, а competitive O. polymorpha glutathione producer was constructed by overexpression of the GSH2 gene, encoding γ-glutamylcysteine synthetase, the first enzyme involved in glutathione biosynthesis, and the MET4 gene coding for central regulator of sulfur metabolism...
March 4, 2018: FEMS Yeast Research
Ping-Wei Ho, Mathias Klein, Matthias Futschik, Elke Nevoigt
Glycerol offers several advantages as a substrate for biotechnological applications. An important step towards using the popular production host Saccharomyces cerevisiae for glycerol-based bioprocesses have been recent studies in which commonly used S. cerevisiae strains were engineered to grow in synthetic medium containing glycerol as the sole carbon source. For metabolic engineering projects of S. cerevisiae growing on glycerol, characterized promoters are missing. In the current study, we used transcriptome analysis and a yECitrine-based fluorescence reporter assay to select and characterize 25 useful promoters...
February 22, 2018: FEMS Yeast Research
Sonoko Tasaki, Tamaki Cho, Jun-Ichi Nagao, Shojiro Ikezaki, Yuka Narita, Ken-Ichi Arita-Morioka, Kanae Yasumatsu, Keita Toyoda, Hiroshi Kojima, Yoshihiko Tanaka
Candida albicans is a human commensal and causes opportunistic infections. Th17 cells provide resistance against mucosal infection with C. albicans; however, the determination of T cell antigens remains little known. Our final goal is to find out the effective T cell antigens of C. albicans responsible for immunotherapy against candidiasis. Here, we prepared some fractions including cytosol, membrane, and cell wall from each yeast and mycelial form-cell. Proteins derived from a membrane fraction of mycelial cells effectively induced differentiation of CD4+ T cells to IL-17A producing Th17 cells...
February 15, 2018: FEMS Yeast Research
Christine Brückner, Mislav Oreb, Gotthard Kunze, Eckhard Boles, Joanna Tripp
A wide range of commercially relevant aromatic chemicals can be synthesized via the shikimic acid pathway. Thus, this pathway has been the target of diverse metabolic engineering strategies. In the present work, an optimized yeast strain for production of the shikimic acid pathway intermediate 3-dehydroshikimate (3-DHS) was generated, which is a precursor for the production of the valuable compounds cis, cis-muconic acid (CCM) and gallic acid (GA). Production of CCM requires the overexpression of the heterologous enzymes 3-DHS dehydratase AroZ, protocatechuic acid (PCA) decarboxylase AroY and catechol dioxygenase CatA...
February 15, 2018: FEMS Yeast Research
Chi Thanh Mai, Quynh Giang Le, Yuki Ishiwata-Kimata, Hiroshi Takagi, Kenji Kohno, Yukio Kimata
Accumulation of unfolded secretory proteins in the endoplasmic reticulum (ER), namely ER stress, is hazardous to eukaryotic cells and promotes the unfolded protein response (UPR). Ire1 is an ER-located transmembrane protein that senses ER stress and triggers the UPR. According to previous in vitro experiments, 4-phenylbutyrate (4-PBA) works as a chemical molecular chaperone. Since 4-PBA attenuates the UPR in mammalian tissue cultures, this chemical may have clinical potential for restoring ER-stressing conditions...
February 14, 2018: FEMS Yeast Research
Meng Zhang, Qilin Yu, Chenpeng Xiao, Kai Zhang, Dan Zhang, Biao Zhang, Mingchun Li
The ability to adapt to environmental changes is the necessary strategy of cell survival. Spt23 is responsible for regulation of Δ-9 desaturase expression in Pichia pastoris. Disruption of SPT23 leads to a remarkable decrease in cellular unsaturated fatty acids. In this study, we found that deletion of SPT23 resulted in growth defect under high temperature culture conditions and heat treatment induced the expression of SPT23. By measuring expression changes of heat shock proteins, protein levels and cellular localization of Hsf1, it revealed that the sensitivity of spt23Δ to high temperature was independent of heat shock response...
February 13, 2018: FEMS Yeast Research
Kostyantyn V Dmytruk, Justyna Ruchala, Dorota Grabek-Lejko, Czeslaw Puchalski, Nina V Bulbotka, Andriy A Sibirny
Lignocellulosic biomass belongs to main sustainable renewable sources for global energy supply. One of the main challenges in the conversion of saccharified lignocellulosic biomass into bioethanol is the utilization of xylose, since lignocellulosic feedstocks contain a significant amount of this pentose. The nonconventional thermotolerant yeast Ogataea polymorpha naturally ferments xylose to ethanol at elevated temperatures (45°C). Studying the molecular mechanisms of regulation of xylose metabolism is a promising way toward increased xylose conversion to ethanol...
February 9, 2018: FEMS Yeast Research
Hannes Juergens, Javier A Varela, Arthur R Gorter de Vries, Thomas Perli, Veronica J M Gast, Nikola Y Gyurchev, Arun S Rajkumar, Robert Mans, Jack T Pronk, John P Morrissey, Jean-Marc G Daran
While CRISPR-Cas9-mediated genome editing has transformed yeast research, current plasmids and cassettes for Cas9 and guide-RNA expression are species specific. CRISPR tools that function in multiple yeast species could contribute to the intensifying research on non-conventional yeasts. A plasmid carrying a pangenomic origin of replication and two constitutive expression cassettes, for Cas9 and a ribozyme-flanked gRNAs was constructed. Its functionality was tested by analyzing inactivation of the ADE2 gene in four yeast species...
February 9, 2018: FEMS Yeast Research
Barreiro-Alonso Aida, Lamas-Maceiras Mónica, Cerdán M Esperanza, Ángel Vizoso-Vázquez
Ixr1 is a Saccharomyces cerevisiae transcriptional factor that extensively regulates the response to hypoxia and controls other important cellular functions and DNA repair. During aerobic growth, the Ixr1 repressor function is predominant on regulated promoters of hypoxic genes, although activator effects are also observed on other genes. During hypoxia, Ixr1 expression increases and the number of genes activated by Ixr1 also increase. In this work we demonstrate that the NH2-terminal region of Ixr1 is involved in transcriptional activation...
February 9, 2018: FEMS Yeast Research
Akula Deepa, Kodipelli Naveena, Roy Anindya
Iron deprivation induces transcription of genes required for iron uptake and transcription factor Aft1 and Aft2 mediate this by regulating transcriptional program in S. cerevisiae. Iron-dependent Fe(II) and 2-oxoglutarate-dependent dioxygenase family proteins are involved in various cellular pathways including DNA alkylation damage repair. Whether Aft1/Aft2 are required for DNA alkylation repair is currently unknown. In this report, we have analyzed DNA alkylation repair under iron-deprived condition. S. cerevisiae Tpa1 is a member of Fe(II) and 2-oxoglutarate-dependent dioxygenase family and we show that deletion of AFT1 and AFT2 genes affects Tpa1 function resulting in sensitivity to alkylating agent MMS...
February 9, 2018: FEMS Yeast Research
Josephine J Peter, Tommaso L Watson, Michelle E Walker, Jennifer M Gardner, Tom A Lang, Anthony Borneman, Angus Forgan, Tina Tran, Vladimir Jiranek
A deficiency of nitrogenous nutrients in grape juice can cause stuck and sluggish alcoholic fermentation, which has long been a problem in winemaking. Nitrogen requirements vary between wine yeast strains, and the ability of yeast to assimilate nitrogen depends on the nature and concentration of nitrogen present in the medium. In this study a wine yeast gene deletion collection (1844 deletants in the haploid AWRI1631 background) was screened to identify genes whose deletion resulted in a reduction in the time taken to utilise all sugars when grown in a chemically defined grape juice medium supplemented with limited nitrogen (75 mg L-1 as a free amino acid mixture)...
February 7, 2018: FEMS Yeast Research
Matti Korhola
I was fortunate to enter yeast research at the Alko Research Laboratories with a strong tradition in yeast biochemistry and physiology studies. At the same time in the 1980s there was a fundamental or paradigm change in molecular biology research with discoveries in DNA sequencing and other analytical and physical techniques for studying macromolecules and cells. Since that time biotechnological research has expanded the traditional fermentation industries to efficient production of e.g. industrial and other enzymes and specialty chemicals...
February 5, 2018: FEMS Yeast Research
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