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FEMS Yeast Research

Roman Maslanka, Magdalena Kwolek-Mirek, Renata Zadrag-Tecza
Glucose plays an important role in cell metabolism and has an impact on cellular physiology. Changes in glucose availability may strongly influence growth rate of the cell size, cell metabolism and the rate of generation of cellular by-products, such as reactive oxygen species. The positive effect of low glucose concentration conditions-calorie restriction is observed in a wide range of species, including the Saccharomyces cerevisiae yeast, yet little is known about the effect of high glucose concentrations-calorie excess...
November 14, 2017: FEMS Yeast Research
Joanna Rytka
No abstract text is available yet for this article.
November 14, 2017: FEMS Yeast Research
Robert Mans, Else-Jasmijn Hassing, Melanie Wijsman, Annabel Giezekamp, Jack T Pronk, Jean-Marc Daran, Antonius J A van Maris
CRISPR/Cas9-based genome editing allows rapid, simultaneous modification of multiple genetic loci in Saccharomyces cerevisiae. Here, this technique was used in a functional analysis study aimed at identifying the hitherto unknown mechanism of lactate export in this yeast. First, an S. cerevisiae strain was constructed with deletions in 25 genes encoding transport proteins, including the complete aqua(glycero)porin family and all known carboxylic-acid transporters. The 25-deletion strain was then transformed with an expression cassette for Lactobacillus casei lactate dehydrogenase (LcLDH)...
November 14, 2017: FEMS Yeast Research
Akaraphol Watcharawipas, Daisuke Watanabe, Hiroshi Takagi
Sodium and acetate inhibit cell growth and ethanol fermentation by different mechanisms in Saccharomyces cerevisiae. We identified the substitution of a conserved Thr255 to Ala (T255A) in the essential Nedd4-family ubiquitin ligase Rsp5, which enhances cellular sodium acetate tolerance. The T255A mutation selectively increased the resistance of cells against sodium acetate, suggesting that S. cerevisiae cells possess an Rsp5-mediated mechanism to cope with the composite stress of sodium and acetate. The sodium acetate tolerance was dependent on the extrusion of intracellular sodium ions by the plasma membrane-localized sodium pumps Ena1, Ena2, and Ena5 (Ena1/2/5) and two known upstream regulators: the Rim101 pH signaling pathway and the Hog1 mitogen-activated protein kinase (MAPK)...
November 2, 2017: FEMS Yeast Research
Ema Cavallo, Hernán Charreau, Patricia Cerrutti, María Laura Foresti
Every year more than 2 million tons of citric acid (CA) are produced around the world for industrial uses. Although initially extracted from citrus, the low profitability of the process and the increasing demand soon stimulated the search for more efficient methods to produce CA. Currently, most world CA demand (99%) is satisfied by fermentations with microorganisms, especially filamentous fungi and yeasts. CA production with yeasts has certain advantages over molds (e.g. higher productivity and easier cultivation), which in the last two decades have triggered a clear increase in publications and patents devoted to the use of yeasts in this field...
November 2, 2017: FEMS Yeast Research
Irina Borodina, Zongbao Kent Zhao
No abstract text is available yet for this article.
November 1, 2017: FEMS Yeast Research
Yueping Zhang, Jens Nielsen, Zihe Liu
Terpenoids represent a large class of natural products with significant commercial applications. These chemicals are currently mainly obtained through extraction from plants and microbes or through chemical synthesis. However, these sources often face challenges of unsustainability and low-productivity. In order to address these issues, Escherichia coli and yeast have been metabolic engineered to produce non-native terpenoids. With recent reports of engineering yeast metabolism to produce several terpenoids at high yields, it has become possible to establish commercial yeast production of terpenoids that find applications as perfume ingredients, pharmaceuticals, and advanced biofuels...
October 31, 2017: FEMS Yeast Research
Xiang Li, Yankun Yang, Chunjun Zhan, Zhenyang Zhang, Xiuxia Liu, Hebin Liu, Zhonghu Bai
The efficient promoter of alcohol oxidase 1 (PAOX1) in methylotrophic yeast Pichia pastoris is strictly induced by methanol but repressed by glycerol with an unclear molecular mechanism. In the present study, the gene of a previously characterized transmembrane protein glycerol transporter 1 (GT1) of P. pastoris GS115 was deleted by homologous recombination. Transcriptional profiles of the mutant (gt1△) and wild-type (WT) were compared with different carbon sources (glycerol, methanol, and glycerol-methanol mix) at various time points using high-throughput RNA-Seq techniques...
October 30, 2017: FEMS Yeast Research
Sara Barbosa Salazar, Can Wang, Martin Musterkotter, Michiyo Okamoto, Azusa Takahashi-Nakaguchi, Hiroji Chibana, Maria Manuel Lopes, Ulrich Güldener, Geraldine Butler, Nuno Pereira Mira
The frequent emergence of azole resistance among Candida glabrata strains contributes to increase the incidence of infections caused by this species. Whole-genome sequencing of a fluconazole and voriconazole-resistant clinical isolate (FFUL887) and subsequent comparison with the genome of the susceptible strain CBS138 revealed prominent differences in several genes documented to promote azole resistance in C. glabrata. Among these was the transcriptional regulator CgPdr1. The CgPdr1 FFUL887 allele included a K274Q modification not documented in other azole-resistant strains...
October 26, 2017: FEMS Yeast Research
Alberto Mercatanti, Samuele Lodovichi, Tiziana Cervelli, Alvaro Galli
Evaluation of functional impact of cancer-associated missense variants is more difficult to assess as compared to protein-truncating mutations and, consequently, standard guidelines for the interpretation of sequence variants have been recently proposed. A number of algorithms and software products were developed to predict the impact of cancer-associated missense mutations on protein structure and function. Importantly, direct assessment of the variants using high-throughput functional assays using simple genetic systems can help in speeding up the functional evaluation of newly identified cancer associated variants...
October 23, 2017: FEMS Yeast Research
Alex N Salazar, Arthur R Gorter de Vries, Marcel van den Broek, Melanie Wijsman, Pilar de la Torre Cortés, Anja Brickwedde, Nick Brouwers, Jean-Marc G Daran, Thomas Abeel
The haploid Saccharomyces cerevisiae strain CEN.PK113-7D is a popular model system for metabolic engineering and systems biology research. Current genome assemblies are based on short-read sequencing data scaffolded based on homology to strain S288C. However, these assemblies contain large sequence gaps, particularly in subtelomeric regions, and the assumption of perfect homology to S288C for scaffolding introduces bias. In this study, we obtained a near-complete genome assembly of CEN.PK113-7D using only Oxford Nanopore Technology's MinION sequencing platform...
September 13, 2017: FEMS Yeast Research
Vasil D'Ambrosio, Michael K Jensen
Our ability to rewire cellular metabolism for the sustainable production of chemicals, fuels and therapeutics based on microbial cell factories has advanced rapidly during the last two decades. Especially the speed and precision by which microbial genomes can be engineered now allow for more advanced designs to be implemented and tested. However, compared to the methods developed for engineering cell factories, the methods developed for testing the performance of newly engineered cell factories in high-throughput are lagging far behind, which consequently impacts the overall biomanufacturing process...
September 13, 2017: FEMS Yeast Research
Jens Nielsen
No abstract text is available yet for this article.
September 11, 2017: FEMS Yeast Research
Ruben Fernandez-Moya, Nancy A Da Silva
Fatty acids and fatty acid derivatives are important biorenewable products, as well as precursors for further transformation via chemical catalysis. This minireview focuses on recent advances in increasing the production of fatty acids and derived products in the yeast Saccharomyces cerevisiae. The engineering of upstream pathways to increase levels of the required precursors, fatty acid synthase systems to increase expression and to modify chain length, and downstream pathways to produce free fatty acids, fatty acid ethyl esters, fatty alcohols, and alkanes are highlighted, and current challenges are discussed...
September 11, 2017: FEMS Yeast Research
Cortés-Acosta Elías, Ibarra J Antonio, Ramírez-Saad Hugo, Vargas-Mendoza C Fabián, Villa-Tanaca Lourdes, Hernández-Rodríguez César
Candida glabrata is an opportunistic fungus infecting mainly immunocompromised people. Its adherence capacity and exoenzymes contribute to damaging host cells. In particular, the yapsins are a family of aspartyl proteases involved in maturation of proteins and cell wall function, and yapsins 1 and 7, respectively encoded by genes CgYPS1 and CgYPS7, are potential virulence factors. In this study, the polymorphism of regulatory regions and the expression profiles of both genes were compared in C. glabrata clinical strains...
December 1, 2017: FEMS Yeast Research
Qun Wu, Kaixiang Cui, Jianchun Lin, Yang Zhu, Yan Xu
Urea is an important intermediate in the synthesis of carcinogenic ethyl carbamate in various food fermentations. Identifying urea-producing microorganisms can help control or reduce ethyl carbamate production. Using Chinese liquor fermentation as a model system, we identified the yeasts responsible for urea production. Urea production was positively correlated to the yeast population (R = 0.523, P = 0.045), and using high-throughput sequencing, we identified 26 yeast species. Partial least squares regression and correlation analysis indicated that Wickerhamomyces anomalus was the most important yeast to produce urea (variable importance plot = 1...
November 1, 2017: FEMS Yeast Research
Juana M Gancedo, Carlos Gancedo
The authors look back at their life together in yeast research, the influences that shaped it, certain challenges and changes in laboratory and funding policies.
November 1, 2017: FEMS Yeast Research
Iva Jancíková, Jakub Zahumenský, Yvetta Gbelská, Dana Gášková
Multidrug transporters are often responsible for failure of medical treatment, since they expel a variety of structurally and functionally unrelated drugs out of the cell. We found that the fluorescent probe diS-C3(3) is a substrate of not only Pdr5p of Saccharomyces cerevisiae (ScPdr5p) but also of its less-explored Kluyveromyces lactis homologue (KlPdr5p). This enabled us to compare the ability of azoles to competitively inhibit the Pdr5p-mediated probe efflux in the two species. In K. lactis, these azoles completely inhibit probe transport by KlPdr5p and also compete with each other for transport...
November 1, 2017: FEMS Yeast Research
Richard J Zahrl, David A Peña, Diethard Mattanovich, Brigitte Gasser
The methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) is one of the most important production systems for heterologous proteins. After the first genome sequences were published in 2009, tremendous effort was made to establish systems-level analytical methods. Methylotrophic lifestyle was one of the most thoroughly investigated topics, studied at the levels of transcriptome, proteome and metabolic flux. Also the responses of P. pastoris to environmental stress conditions experienced during high cell density production processes were studied...
November 1, 2017: FEMS Yeast Research
Sheng Wang, Ruishan Wang, Tan Liu, Zhilai Zhan, Liping Kang, Yanan Wang, Chaogeng Lv, Daniele Werck-Reichhart, Lanping Guo, Luqi Huang
Shikonin and its derivatives are the main active components in the medicinal plant Arnebia euchroma and possess extensive pharmaceutical properties. In this study, we developed an optimized yeast system to obtain high-level production of 3-geranyl-4-hydroxybenzoate acid (GBA), an important intermediate involved in shikonin biosynthesis pathway. For host selection, recombinant expression of p-hydroxybenzoate:geranyltransferase (PGT) derived from A. euchroma was performed in Saccharomyces cerevisiae WAT11U strain and high yield monoterpene strain...
November 1, 2017: FEMS Yeast Research
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