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FEMS Yeast Research

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https://www.readbyqxmd.com/read/28199631/lack-of-cortical-endoplasmic-reticulum-protein-ist2-alters-sodium-accumulation-in-saccharomyces-cerevisiae-cells
#1
Klara Papouskova, Marketa Andrsova, Hana Sychrova
No abstract text is available yet for this article.
February 11, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28175306/dynamic-modeling-reveals-a-three-step-response-of-s-cerevisiae-to-high-co2-levels-accompanied-by-increasing-atp-demands
#2
Gerhard Eigenstetter, Ralf Takors
No abstract text is available yet for this article.
February 8, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28175291/selection-of-non-saccharomyces-yeasts-to-be-used-in-grape-musts-with-high-alcoholic-potential-a-strategy-to-obtain-wines-with-reduced-ethanol-content
#3
M Victoria Mestre, Y Paola Maturano, Mariana Combina, Laura A Mercado, M Eugenia Toro, Fabio Vazquez
No abstract text is available yet for this article.
February 8, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28158539/defects-in-methylation-of-arginine-37-on-cenp-a-cse4-are-compensated-by-the-ubiquitin-ligase-complex-ubr2-mub1
#4
Anke Samel, Thi Kim Loan Nguyen, Ann E Ehrenhofer-Murray
No abstract text is available yet for this article.
February 2, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28158422/role-of-phosphatidylserine-synthase-in-shaping-the-phospholipidome-of-candida-albicans
#5
Chelsi D Cassilly, Abigail T Farmer, Anthony E Montedonico, Terry K Smith, Shawn R Campagna, Todd B Reynolds
No abstract text is available yet for this article.
February 2, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28087675/dna-repair-and-mutations-during-quiescence-in-yeast
#6
Serge Gangloff, Benoit Arcangioli
Life is maintained through alternating phases of cell division and quiescence. The causes and consequences of spontaneous mutations have been extensively explored in proliferating cells, and the major sources include errors of DNA replication and DNA repair. The foremost consequences are genetic variations within a cell population that can lead to heritable diseases and drive evolution. While most of our knowledge on DNA damage response and repair has been gained through cells actively dividing, it remains essential to also understand how DNA damage is metabolized in cells which are not dividing...
January 12, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28087674/yeast-as-a-tool-to-express-sugar-acid-transporters-with-biotechnological-interest
#7
David Ribas, Joana Sá-Pessoa, Isabel Soares-Silva, Sandra Paiva, Yvonne Nygård, Laura Ruohonen, Merja Penttilä, Margarida Casal
Sugar acids can be used as platform chemicals to generate primary building blocks of industrially relevant products. Microbial production of these organic compounds at high yields requires the engineering of the enzymatic machinery and the presence of plasma membrane transporters able to export them outside the cells. In this study several yeast carboxylic acid transporters belonging to the Jen family were screened for the transport of biotechnologically relevant sugar acids, namely gluconic, saccharic, mucic, xylaric and xylonic acid, and functionally characterized in Saccharomyces cerevisiae We show that Jen permeases are capable of transporting most of these sugar acids, although with different specificities...
January 12, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28087673/rdna-stability-is-supported-by-many-buffer-genes-introduction-to-the-yeast-rdna-stability-database
#8
Takehiko Kobayashi, Mariko Sasaki
The ribosomal RNA gene (rDNA) is the most abundant gene in yeast and other eukaryotic organisms. Due to its heavy transcription, repetitive structure and programmed replication fork pauses, the rDNA is one of the most unstable regions in the genome. Thus, the rDNA is the best region to study the mechanisms responsible for maintaining genome integrity. Recently, we screened a library of ∼4,800 budding yeast gene knockout strains to identify mutants defective in the maintenance of rDNA stability. The results of this screen are summarized in the Yeast rDNA Stability Database, in which the stability and copy number of rDNA in each mutant are presented...
January 12, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28087672/elimination-of-sucrose-transport-and-hydrolysis-in-saccharomyces-cerevisiae-a-platform-strain-for-engineering-sucrose-metabolism
#9
Wesley Leoricy Marques, Robert Mans, Eko Roy Marella, Rosa Lorizolla Cordeiro, Marcel van den Broek, Jean-Marc G Daran, Jack T Pronk, Andreas K Gombert, Antonius J A van Maris
Many relevant options to improve efficacy and kinetics of sucrose metabolism in Saccharomyces cerevisiae and, thereby, the economics of sucrose-based processes, remain to be investigated. An essential first step is to identify all native sucrose-hydrolysing enzymes and sucrose transporters in this yeast, including those that can be activated by suppressor mutations in sucrose-negative strains. A strain in which all known sucrose-transporter genes (MAL11, MAL21, MAL31, MPH2, MPH3) were deleted did not grow on sucrose after 2 months of incubation...
January 12, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28073930/editorial-3rs-tightly-intertwined-to-maintain-genome-stability
#10
EDITORIAL
Michael Lisby, Uffe H Mortensen
No abstract text is available yet for this article.
January 10, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28073929/improving-heterologous-production-of-phenylpropanoids-in-saccharomyces-cerevisiae-by-tackling-an-unwanted-side-reaction-of-tsc13-an-endogenous-double-bond-reductase
#11
Beata Joanna Lehka, Michael Eichenberger, Walden Emil Bjørn-Yoshimoto, Katherina Garcia Vanegas, Nicolaas Buijs, Niels Bjerg Jensen, Jane Dannow Dyekjær, Håvard Jenssen, Ernesto Simon, Michael Naesby
Phenylpropanoids, such as flavonoids and stilbenoids, are of great commercial interest, and their production in S. cerevisiae is a very promising strategy. However, to achieve commercially viable production, each step of the process must be optimized. We looked at carbon loss, known to occur in the heterologous flavonoid pathway in yeast, and identified an endogenous enzyme, the enoyl reductase Tsc13, which turned out to be responsible for the accumulation of phloretic acid via reduction of p-coumaroyl-CoA...
January 10, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/27993914/advances-and-challenges-in-logical-modeling-of-cell-cycle-regulation-perspective-for-multi-scale-integrative-yeast-cell-models
#12
Matteo Barberis, Robert G Todd, Lucas van der Zee
The eukaryotic cell cycle is robustly designed, with interacting molecules organized within a definite topology that ensures temporal precision of its phase transitions. Its underlying dynamics are regulated by molecular switches, for which remarkable insights have been provided by genetic and molecular biology efforts. In a number of cases, this information has been made predictive, through computational models. These models have allowed for the identification of novel molecular mechanisms, later validated experimentally...
January 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28011906/saccharomyces-uvarum-is-responsible-for-the-traditional-fermentation-of-apple-chicha-in-patagonia
#13
M E Rodríguez, L Pérez-Través, M P Sangorrín, E Barrio, A Querol, C A Lopes
Apple chicha is a fresh low alcoholic beverage elaborated by aboriginal communities of Andean Patagonia (Argentina and Chile). In the present work we identified the yeast microbiota associated with this fermentation, and characterized genetically those belonging to the genus Saccharomyces Both Saccharomyces cerevisiae and Saccharomyces uvarum were found in the analyzed fermentations. Phylogenetic and population structure analyses based on genes sequence analysis were carried out for both S. cerevisiae and S...
December 23, 2016: FEMS Yeast Research
https://www.readbyqxmd.com/read/28011905/replication-fork-regression-and-its-regulation
#14
Xiangzhou Meng, Xiaolan Zhao
One major challenge during genome duplication is the stalling of DNA replication forks by various forms of template blockages. As these barriers can lead to incomplete replication, multiple mechanisms have to act concertedly to correct and rescue stalled replication forks. Among these mechanisms, replication fork regression entails simultaneous annealing of nascent and template strands, which leads to regression of replication forks and formation of four-way DNA junctions. In principle, this process can lead to either positive outcomes, such as DNA repair and replication resumption, or less desirable outcomes, such as misalignment between nascent and template DNA and DNA cleavage...
December 23, 2016: FEMS Yeast Research
https://www.readbyqxmd.com/read/28011904/multifunctional-roles-of-saccharomyces-cerevisiae-srs2-protein-in-replication-recombination-and-repair
#15
Hengyao Niu, Hannah L Klein
The Saccharomyces cerevisiae Srs2 DNA helicase has important roles in DNA replication, recombination and repair. In replication, Srs2 aids in repair of gaps by repair synthesis by preventing gaps from being used to initiate recombination. This is considered to be an anti-recombination role. In recombination, Srs2 plays both pro-recombination and anti-recombination roles to promote the synthesis-dependent strand annealing (SDSA) recombination pathway and to inhibit gaps from initiating homologous recombination (HR)...
December 23, 2016: FEMS Yeast Research
https://www.readbyqxmd.com/read/27993913/direct-stamp-of-technology-or-origin-on-the-genotypic-and-phenotypic-variation-of-indigenous-saccharomyces-cerevisiae-population-in-a-natural-model-of-boiled-grape-juice-fermentation-into-traditional-msalais-wine-in-china
#16
Li-Xia Zhu, Guan-Qiong Wang, Ju-Lan Xue, Dong-Qi Gou, Chang-Qing Duan
Saccharomyces cerevisiae strains worldwide show genetic and phenotypic diversity and their population substructures are greatly affected by their technological application or geographical origins. Msalais is a traditional wine obtained via a unique method of spontaneous fermentation of local boiled grape juice in Southern Xinjiang. We analyzed 436 indigenous S. cerevisiae strains associated with Msalais fermentation. These strains were highly diverse with respect to the inter-delta region and 24 phenotypic traits, with apparent differentiation according to strain origins and technologies used to produce Msalais...
December 18, 2016: FEMS Yeast Research
https://www.readbyqxmd.com/read/27956494/sch-9p-kinase-and-the-gcn4p-transcription-factor-regulate-glycerol-production-during-winemaking
#17
Beatriz Vallejo, Helena Orozco, Cecilia Picazo, Emilia Matallana, Agustín Aranda
Grape juice fermentation is a harsh environment with many stressful conditions, and Saccharomyces cerevisiae adapts its metabolism in response to those environmental challenges. Many nutrient-sensing pathways control this feature. The Tor/Sch 9p pathway promotes growth and protein synthesis when nutrients are plenty, while the transcription factor Gcn4p is required for the activation of amino acid biosynthetic pathways. We previously showed that Sch 9p impact on longevity depends on the nitrogen/carbon ratio...
December 11, 2016: FEMS Yeast Research
https://www.readbyqxmd.com/read/27956493/laura-frontali-my-life-with-yeast
#18
Laura Frontali
No abstract text is available yet for this article.
December 11, 2016: FEMS Yeast Research
https://www.readbyqxmd.com/read/27956492/screening-for-novel-genes-of-saccharomyces-cerevisiae-involved-in-recombinant-antibody-production
#19
Jorg C de Ruijter, German Jurgens, Alexander D Frey
Cost-effective manufacturing of biopharmaceuticals in non-mammalian hosts still requires tremendous efforts in strain development. In order to expedite identification of novel leads for strain engineering, we used a transposon-mutagenized yeast genomic DNA library to create a collection of Saccharomyces cerevisiae deletion strains expressing a full-length IgG antibody. Using a high-throughput screening, transformants with either significantly higher or lower IgG expression were selected. The integration site of the transposon in three of the selected strains was located by DNA sequencing...
December 11, 2016: FEMS Yeast Research
https://www.readbyqxmd.com/read/27956491/fermentation-assays-reveal-differences-in-sugar-and-off-flavor-metabolism-across-different-brettanomyces-bruxellensis-strains
#20
Sam Crauwels, Filip Van Opstaele, Barbara Jaskula-Goiris, Jan Steensels, Christel Verreth, Lien Bosmans, Caroline Paulussen, Beatriz Herrera-Malaver, Ronnie de Jonge, Jessika De Clippeleer, Kathleen Marchal, Gorik De Samblanx, Kris A Willems, Kevin J Verstrepen, Guido Aerts, Bart Lievens
Brettanomyces (Dekkera) bruxellensis is an ascomycetous yeast of major importance in the food, beverage and biofuel industry. It has been isolated from various man-made ecological niches that are typically characterized by harsh environmental conditions such as wine, beer, soft drink etc. Recent comparative genomics studies revealed an immense intraspecific diversity, but it is still unclear whether this genetic diversity also leads to systematic differences in fermentation performance and (off-)flavor production, and to what extent strains have evolved to match their ecological niche...
December 11, 2016: FEMS Yeast Research
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