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FEMS Yeast Research

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https://www.readbyqxmd.com/read/28637316/incorporating-comparative-genomics-into-the-design-test-learn-cycle-of-microbial-strain-engineering
#1
Maria Sardi, Audrey P Gasch
Engineering microbes with new properties is an important goal in industrial engineering, to establish biological factories for production of biofuels, commodity chemicals, and pharmaceutics. But engineering microbes to produce new compounds with high yield remains a major challenge toward economically viable production. Incorporating several modern approaches, including synthetic and systems biology, metabolic modeling, and regulatory rewiring have proven to significantly advance industrial strain engineering...
June 16, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28637280/networking-and-collaboration-a-stronghold-of-the-yeast-research-community
#2
John Morrissey
No abstract text is available yet for this article.
June 15, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28637248/expression-and-comparison-of-codon-optimised-aspergillus-tubingensis-amylase-variants-in-saccharomyces-cerevisiae
#3
Rosemary A Cripwell, Shaunita H Rose, Willem H van Zyl
The expression of codon optimised genes is a popular genetic engineering approach for the production of industrially relevant proteins. This study investigates and compares the expression of codon optimised and codon adapted amylase variants. The Aspergillus tubingensis raw starch hydrolysing α-amylase (amyA) and glucoamylase (glaA) encoding genes were redesigned using synonymous codons and expressed in S. cerevisiae Y294. Codon optimisation to favour S. cerevisiae codon bias resulted in a decrease in extracellular enzyme activity of 72% (30...
June 15, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28633406/in-saccharomyces-cerevisiae-gene-targeting-fidelity-depends-on-a-transformation-method-and-proportion-of-the-overall-length-of-the-transforming-and-targeted-dna
#4
Anamarija Štafa, Marina Svetec Miklenic, Antonio Zandona, Bojan Žunar, Neža Cadež, Hrvoje Petkovic, Ivan Krešimir Svetec
Gene replacement is one of the most essential approaches in construction of the genetically modified yeast strains. However, the fidelity of gene targeting and the effort needed for construction of a particular strain can vary significantly. We investigated the influence of two important factors-the choice of the transformation method and the design of the transforming DNA fragment, which can vary in overall length (including flanking regions and selectable marker) compared to the length of the targeted region in the genome...
June 15, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28586408/engineering-tolerance-to-industrially-relevant-stress-factors-in-yeast-cell-factories
#5
Quinten Deparis, Arne Claes, Maria R Foulquié-Moreno, Johan M Thevelein
The main focus in development of yeast cell factories has generally been on establishing optimal activity of heterologous pathways and further metabolic engineering of the host strain to maximize product yield and titer. Adequate stress tolerance of the host strain has turned out to be another major challenge for obtaining economically viable performance in industrial production. Although general robustness is a universal requirement for industrial micro-organisms, production of novel compounds using artificial metabolic pathways presents additional challenges...
June 6, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28582501/what-do-the-pictures-say-snapshots-of-a-career
#6
Terrance G Cooper
What follows are snapshots of my career in chicken eyes, yeast and R. rubrum, castor beans, E. coli, and finally yeast again. In contrast, only a few of the failures that realistically make up a career are included. It is a tale of the generosity and influences of those who shaped what I am and what I learned in a wonderful profession. The science described is only that which I was lucky enough to do or was performed in my laboratory by those who really deserve the credit for any success that I've enjoyed. Not mentioned for lack of space are the critical contributions of many impressive investigators in the field of nitrogen-responsive regulation for no scientific investigation occurs in isolation...
June 4, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28582498/microorganism-design-for-heterologous-biosynthesis-of-cannabinoids
#7
Carvalho Ângela, Esben Halkjær Hansen, Oliver Kayser, Simon Carlsen, Felix Stehle
During the last decade the use of medical Cannabis has expanded globally and legislation is getting more liberal in many countries, facilitating the research on cannabinoids. The unique interaction of cannabinoids with the human endocannabinoid system make these compounds an interesting target to be studied as a therapeutic agent for the treatment of several medical conditions. However, currently there are important limitations in the study, production and use of cannabinoids as pharmaceutical drugs. Besides the main constituent tetrahydrocannabinolic acid, the structurally related compound cannabidiol is of high interest as drug candidate...
June 4, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28582494/engineering-of-saccharomyces-cerevisiae-for-the-efficient-co-utilization-of-glucose-and-xylose
#8
Jin Hou, Chenxi Qiu, Yu Shen, Hongxing Li, Xiaoming Bao
The rapid co-fermentation of both glucose and xylose is important for the efficient conversion of lignocellulose biomass into fuels and chemicals. Saccharomyces cerevisiae is considered to be a potential cell factory and has been used to produce various fuels and chemicals, but it cannot metabolize xylose, which has greatly limited the utilization of lignocellulose materials. Therefore, numerous studies have attempted to develop xylose fermenting strains in past decades. The simple introduction of the xylose metabolic pathway does not enable yeast to rapidly utilize xylose, and several limitations still need to be addressed, including glucose repression and slow xylose transport, cofactor imbalance in the xylose reductase/xylitol dehydrogenase pathway, functional expression of a heterologous xylose isomerase, the low efficiency of downstream pathways and low ethanol production...
June 4, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28582493/new-yeasts-new-brews-modern-approaches-to-brewing-yeast-design-and-development
#9
B Gibson, J-M A Geertman, C T Hittinger, K Krogerus, D Libkind, E J Louis, F Magalhães, J P Sampaio
The brewing industry is experiencing a period of change and experimentation largely driven by customer demand for product diversity. This has coincided with a greater appreciation of the role of yeast in determining the character of beer and the widespread availability of powerful tools for yeast research. Genome analysis in particular has helped clarify the processes leading to domestication of brewing yeast and has identified domestication signatures that may be exploited for further yeast development. The functional properties of non-conventional yeast (both Saccharomyces and non-Saccharomyces) are being assessed with a view to creating beers with new flavours as well as producing flavoursome non-alcoholic beers...
June 4, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28582489/pathway-engineering-for-the-production-of-heterologous-aromatic-chemicals-and-their-derivatives-in-saccharomyces-cerevisiae-bioconversion-from-glucose
#10
Manuela Gottardi, Mara Reifenrath, Eckhard Boles, Joanna Tripp
Saccharomyces cerevisiae has been extensively engineered for optimizing its performance as a microbial cell factory to produce valuable aromatic compounds and their derivatives as bulk and fine chemicals. The production of heterologous aromatic molecules in yeast is achieved via engineering of the aromatic amino acid biosynthetic pathway. This pathway is connected to two pathways of the central carbon metabolism, and is highly regulated at the gene and protein level. These characteristics impose several challenges for tailoring it, and various modifications need to be applied in order to redirect the carbon flux towards the production of the desired compounds...
June 4, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28817926/yeast-multistress-resistance-and-lag-phase-characterisation-during-wine-fermentation
#11
David Ferreira, Virginie Galeote, Isabelle Sanchez, Jean-Luc Legras, Anne Ortiz-Julien, Sylvie Dequin
Saccharomyces cerevisiae has been used to perform wine fermentation for several millennia due to its endurance and unmatched qualities. Nevertheless, at the moment of inoculation, wine yeasts must cope with specific stress factors that still challenge wine makers by slowing down or compromising the fermentation process. To better assess the role of genetic and environmental factors that govern multistress resistance during the wine fermentation lag phase, we used a factorial plan to characterise the individual and combined impact of relevant stress factors on eight S...
September 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28810705/flowers-as-a-reservoir-of-yeast-diversity-description-of-wickerhamiella-nectarea-f-a-sp-nov-and-wickerhamiella-natalensis-f-a-sp-nov-from-south-african-flowers-and-pollinators-and-transfer-of-related-candida-species-to-the-genus-wickerhamiella-as-new-combinations
#12
Clara de Vega, Rafael G Albaladejo, Beatriz Guzmán, Sandy-Lynn Steenhuisen, Steven D Johnson, Carlos M Herrera, Marc-André Lachance
Flowers offer favourable microenvironments for yeast growth, and are increasingly recognised as a rich source of novel yeast species. Independent surveys of yeasts associated with flowers and pollinators in South Africa led to the discovery of 38 strains of two new species. Physiological profiles and analysis of the internal transcribed spacer and the D1/D2 domains of the large subunit rRNA gene showed that they represent two novel species that belong to the Wickerhamiella clade. We describe the species as Wickerhamiella nectarea f...
August 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28810704/yeast-kch1-and-kch2-membrane-proteins-play-a-pleiotropic-role-in-membrane-potential-establishment-and-monovalent-cation-homeostasis-regulation
#13
Kristina Felcmanova, Petra Neveceralova, Hana Sychrova, Olga Zimmermannova
The Kch1 and Kch2 plasma-membrane proteins were identified in Saccharomyces cerevisiae as being essential for the activation of a high-affinity Ca2+ influx system. We searched for Kch proteins roles in the maintenance of cation homeostasis and tested the effect of kch1 and/or kch2 deletions on various physiological parameters. Compared to wild-type, kch1 kch2 mutant cells were smaller, relatively hyperpolarised, grew better under limited K+ conditions and exhibited altered growth in the presence of monovalent cations...
August 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28810703/exploring-the-potential-of-saccharomyces-eubayanus-as-a-parent-for-new-interspecies-hybrid-strains-in-winemaking
#14
Frederico Magalhães, Kristoffer Krogerus, Sandra Castillo, Anne Ortiz-Julien, Sylvie Dequin, Brian Gibson
Yeast cryotolerance brings some advantages for wine fermentations, including the improved aromatic complexity of white wines. Naturally cold-tolerant strains are generally less adept at wine fermentation but fermentative fitness can potentially be improved through hybridization. Here we studied the potential of using hybrids involving Saccharomyces eubayanus and a S. cerevisiae wine strain for low-temperature winemaking. Through screening the performance in response to variable concentrations of sugar, nitrogen and temperature, we isolated one hybrid strain that exhibited the superior performance...
August 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28810702/the-nutrient-transceptor-pka-pathway-functions-independently-of-tor-and-responds-to-leucine-and-gcn2-in-a-tor-independent-manner
#15
Michaela Conrad, Harish Nag Kankipati, Marlies Kimpe, Griet Van Zeebroeck, Zhiqiang Zhang, Johan M Thevelein
Two nutrient-controlled signalling pathways, the PKA and TOR pathway, play a major role in nutrient regulation of growth as well as growth-correlated properties in yeast. The relationship between the two pathways is not well understood. We have used Gap1 and Pho84 transceptor-mediated activation of trehalase and phosphorylation of fragmented Sch9 as a read-out for rapid nutrient activation of PKA or TORC1, respectively. We have identified conditions in which L-citrulline-induced activation of Sch9 phosphorylation is compromised, but not activation of trehalase: addition of the TORC1 inhibitor, rapamycin and low levels of L-citrulline...
August 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28810701/yeast-genes-involved-in-regulating-cysteine-uptake-affect-production-of-hydrogen-sulfide-from-cysteine-during-fermentation
#16
Chien-Wei Huang, Michelle E Walker, Bruno Fedrizzi, Richard C Gardner, Vladimir Jiranek
An early burst of hydrogen sulfide (H2S) produced by Saccharomyces cerevisiae during fermentation could increase varietal thiols and therefore enhance desirable tropical aromas in varieties such as Sauvignon Blanc. Here we attempted to identify genes affecting H2S formation from cysteine by screening yeast deletion libraries via a colony colour assay on media resembling grape juice. Both Δlst4 and Δlst7 formed lighter coloured colonies and produced significantly less H2S than the wild type on high concentrations of cysteine, likely because they are unable to take up cysteine efficiently...
August 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28633312/the-influence-of-dekkera-bruxellensis-on-the-transcriptome-of-saccharomyces-cerevisiae-and-on-the-aromatic-profile-of-synthetic-wine-must
#17
Janez Kosel, Neža Cadež, Dorit Schuller, Laura Carreto, Ricardo Franco-Duarte, Peter Raspor
A double compartment membrane system was constructed in order to systematically study possible microbial interactions between yeasts Saccharomyces cerevisiae and Dekkera bruxellensis and their impact on wine aroma. The presence of D. bruxellensis induced 77 transcripts of S. cerevisiae. These were mostly of unknown function; however, some were involved in thiamine biosynthesis and in amino acid and polyamine transport, suggesting a competitive relationship between the two yeast species. Among the transcripts with no biological function, 14 of them were found to be the members of the PAU gene family that is associated with response to anaerobiosis stress...
June 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28520879/phylogenetic-relationship-and-fourier-transform-infrared-spectroscopy-derived-lipid-determinants-of-lifespan-parameters-in-the-saccharomyces-cerevisiae-yeast
#18
Mateusz Molon, Jacek Zebrowski
Yeast ageing has been gaining much attention in gerontology research, yet the process itself is still not entirely clear. One of the constraints related to the use of the Saccharomyces cerevisiae yeast in studies is the ambiguity of the results concerning ageing determinants for different genetic backgrounds. In this paper, we compare reproductive potentials and lifespans of seven widely used haploid laboratory strains differing in daughter cells production capabilities and highlight the importance of choosing an appropriate genotype for the studies on ageing...
June 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28505300/impact-of-glutathione-metabolism-on-zinc-homeostasis-in-saccharomyces-cerevisiae
#19
Matthias G Steiger, Anett Patzschke, Caterina Holz, Christine Lang, Tim Causon, Stephan Hann, Diethard Mattanovich, Michael Sauer
Zinc is a crucial mineral for all organisms as it is an essential cofactor for the proper function of a plethora of proteins and depletion of zinc causes oxidative stress. Glutathione is the major redox buffering agent in the cell and therefore important for mitigation of the adverse effects of oxidative stress. In mammalian cells, zinc deficiency is accompanied by a glutathione depletion. In the yeast Saccharomyces cerevisiae, the opposite effect is observed: under low zinc conditions, an elevated glutathione concentration is found...
June 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28334164/transcription-factor-mxr1-promotes-the-expression-of-aox1-by-repressing-glycerol-transporter-1-in-pichia-pastoris
#20
Chunjun Zhan, Yankun Yang, Zhenyang Zhang, Xiang Li, Xiuxia Liu, Zhonghu Bai
In the methylotrophic yeast Pichia pastoris (P. pastoris), the efficient promoter of alcohol oxidase (PAox1) is induced by methanol and repressed by glycerol, but the molecular mechanism is not clear. In this study, the relationship between alcohol oxidase 1 (aox1), methanol expression regulator 1 (mxr1) and glycerol transporter 1 (gt1) was studied. By RT-PCR, it was found that the overexpression of gt1 could increase the glycerol content in cells and repress the expression of mxr1 and aox1, and the deletion of gt1 reduced the glycerol content in cells and promoted the expression of aox1...
June 1, 2017: FEMS Yeast Research
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