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FEMS Yeast Research

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https://www.readbyqxmd.com/read/28520895/distribution-patterns-of-saccharomyces-species-in-cultural-landscapes-of-germany
#1
Michael Brysch-Herzberg, Martin Seidel
The distribution patterns of the three Saccharomyces species, S. paradoxus, S. uvarum and S. cerevisiae, were investigated by a culture-dependent approach in order to understand better how these species propagate in the cultural landscape of Germany. Saccharomyces paradoxus, the closest relative of S. cerevisiae, is shown to be a true woodland species. It was frequentely found in the soil under conifers indicating that S. paradoxus is an autochthonous member of the microbial community in this habitat. Physiological characteristics of the species like the Crabtree effect and high tolerance against ethanol suggest that the species is adapted to regular supply with considerable amounts of sugars...
May 17, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28520879/phylogenetic-relationship-and-ftir-spectroscopy-derived-lipid-determinants-of-lifespan-parameters-in-the-saccharomyces-cerevisiae-yeast
#2
Mateusz Molon, Jacek Zebrowski
Yeast aging has been gaining much attention in gerontology research, yet the process itself is still not entirely clear. One of the constraints related to the use of the Saccharomyces cerevisiae yeast in studies is the ambiguity of the results concerning aging determinants for different genetic backgrounds. In this paper, we compare reproductive potentials and lifespans of seven widely used haploid laboratory strains differing in daughter cells production capabilities and highlight the importance of choosing an appropriate genotype for the studies on aging...
May 17, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28505256/crispr-cas-system-for-yeast-genome-engineering-advances-and-applications
#3
Vratislav Stovicek, Carina Holkenbrink, Irina Borodina
The methods based on the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system have quickly gained popularity for genome editing and transcriptional regulation in many organisms, including yeast. This review aims to provide a comprehensive overview of CRISPR application for different yeast species: from basic principles and genetic design to applications.
May 15, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28505306/secretion-of-2-3-dihydroxyisovalerate-as-a-limiting-factor-for-isobutanol-production-in-saccharomyces-cerevisiae
#4
Wesley Cardoso Generoso, Martin Brinek, Heiko Dietz, Mislav Oreb, Eckhard Boles
Isobutanol is a superior biofuel compared to ethanol, and it is naturally produced by yeasts. Previously, Saccharomyces cerevisiae has been genetically engineered to improve isobutanol production. We found that yeast cells engineered for a cytosolic isobutanol biosynthesis secrete large amounts of the intermediate 2,3-dihydroxyisovalerate (DIV). This indicates that the enzyme dihydroxyacid dehydratase (Ilv3) is limiting the isobutanol pathway and/or yeast exhibit effective transport systems for the secretion of the intermediate, competing with isobutanol synthesis...
May 12, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28505300/impact-of-glutathione-metabolism-on-zinc-homeostasis-in-saccharomyces-cerevisiae
#5
Matthias G Steiger, Anett Patzschke, Caterina Holz, Christine Lang, Tim Causon, Stephan Hann, Diethard Mattanovich, Michael Sauer
Zinc is a crucial mineral for all organisms as it is an essential cofactor for the proper function of a plethora of proteins and depletion of zinc causes oxidative stress. Glutathione is the major redox buffering agent in the cell and therefore important for mitigation of the adverse effects of oxidative stress. In mammalian cells, zinc deficiency is accompanied by a glutathione depletion. In the yeast Saccharomyces cerevisiae, the opposite effect is observed: under low zinc conditions an elevated glutathione concentration is found...
May 12, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28475763/cell-wall-canals-formed-upon-growth-of-%C3%B0-andida-maltosa-in-the-presence-of-hexadecane-are-associated-with-polyphosphates
#6
Anton N Zvonarev, David E Crowley, Lubov P Ryazanova, Lydia P Lichko, Tatiana G Rusakova, Tatiana V Kulakovskaya, Vladimir V Dmitriev
Canals are supramolecular complexes observed in the cell wall of Candida maltosa grown in the presence of hexadecane as a sole carbon source. Such structures were not observed in glucose grown cells. Microscopic observations of cells stained with diaminobenzidine revealed the presence of oxidative enzymes in the canals. DAPI staining revealed that a substantial part of cellular polyphosphate was present in the cell wall of cells grown on hexadecane in condition of phosphate limitation. The content and chain length of polyphosphates were higher in hexadecane-grown cells than in glucose grown ones...
May 4, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28472279/the-genetic-architecture-in-saccharomyces-cerevisiae-that-contributes-to-variation-in-drug-response-to-the-antifungals-benomyl-and-ketoconazole
#7
Christina A Roberts, John H Miller, Paul H Atkinson
The genetic basis of variation in drug response was investigated in individual Saccharomyces cerevisiae strains that exhibited different susceptibility to two antifungal agents-benomyl and ketoconazole. Following dose-response screening of 25 strains, four were selected on the basis of resistance or sensitivity relative to the standard laboratory strain BY. UWOPS87-2421 and L-1374 were respectively resistant and sensitive to benomyl; DBVPG6044 and Y12 were respectively resistant and sensitive to ketoconazole...
May 2, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28460089/genome-sequence-of-the-highly-weak-acid-tolerant-zygosaccharomyces-bailii-ist302-amenable-to-genetic-manipulations-and-physiological-studies
#8
Margarida Palma, Martin Münsterkötter, João Peça, Ulrich Güldener, Isabel Sá-Correia
Zygosaccharomyces bailii is one of the most problematic spoilage yeast species found in the food and beverage industry particularly in acidic products, due to its exceptional resistance to weak acid stress. This article describes the annotation of the genome sequence of Z. bailii IST302, a strain recently proven to be amenable to genetic manipulations and physiological studies. The work was based on the annotated genomes of strain ISA1307, an interspecies hybrid between Z. bailii and a closely related species, and the Z...
April 29, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28460027/saccharomyces-riding-the-waves-of-technology-and-transition
#9
Terrance G Cooper
No abstract text is available yet for this article.
April 27, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28449102/enhancing-expression-of-ssu1-genes-in-saccharomyces-uvarum-leads-to-an-increase-in-sulfite-tolerance-and-transcriptome-profiles-change
#10
X Z Liu, M Sang, X A Zhang, T K Zhang, H Y Zhang, X He, S X Li, X D Sun, Z M Zhang
Saccharomyces uvarum is a good wine yeast species which may have great potential for the future. However, sulfur tolerance of most S. uvarum strains is very poor. In addition there is still little information about the SSU1 gene of S. uvarum. In order to analyze the function of SSU1 genes, two expression vectors which contained different SSU1 genes were constructed and transferred into a sulfite-tolerant S. uvarum strain A9. Then sulfite tolerance, PCR analysis, sequencing analysis, SO2 output production analysis, RT-qPCR analysis and transcriptome analysis were used to access the function of the S...
April 25, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28449083/proteomic-response-to-linoleic-acid-hydroperoxide-in-saccharomyces-cerevisiae
#11
Patrick J O'Doherty, Alamgir Khan, Adam J Johnson, Peter J Rogers, Trevor D Bailey, Ming J Wu
Yeast AP-1 transcription factor (Yap1p) and the enigmatic oxidoreductases Oye2p and Oye3p are involved in counteracting lipid oxidants and their unsaturated breakdown products. In order to uncover the response to linoleic acid hydroperoxide (LoaOOH) and the roles of Oye2p, Oye3p, and Yap1p, we carried out proteomic analysis of the homozygous deletion mutants oye3Δ, oye2Δ, and yap1Δ alongside the diploid parent strain BY4743. The findings demonstrate that deletion of YAP1 narrowed the response to LoaOOH, as the number of proteins differentially expressed in yap1Δ was 70% of that observed in BY4743...
April 25, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28419226/sugar-versus-fat-elimination-of-glycogen-storage-improves-lipid-accumulation-in-yarrowia-lipolytica
#12
Govindprasad Bhutada, Martin Kavšcek, Rodrigo Ledesma-Amaro, Stéphane Thomas, Gerald N Rechberger, Jean-Marc Nicaud, Klaus Natter
Triacylglycerol (TAG) and glycogen are the two major metabolites for carbon storage in most eukaryotic organisms. We investigated the glycogen metabolism of the oleaginous Y. lipolytica and found that this yeast accumulates up to 16% glycogen in its biomass. Assuming that elimination of glycogen synthesis would result in an improvement of lipid accumulation, we characterized and deleted the single gene coding for glycogen synthase, YlGSY1. The mutant was grown under lipogenic conditions with glucose and glycerol as substrates and we obtained up to 60% improvement in TAG accumulation compared to the wild type strain...
April 17, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28334329/metabolomics-of-pichia-pastoris-impact-of-buffering-conditions-on-the-kinetics-and-nature-of-metabolite-loss-during-quenching
#13
Matthias Mattanovich, Hannes Rußmayer, Theresa Scharl-Hirsch, Verena Puxbaum, Jonas Burgard, Diethard Mattanovich, Stephan Hann
Mass spectrometry based metabolomic profiling is a powerful strategy to quantify the concentrations of numerous primary metabolites in parallel. To avoid distortion of metabolite concentrations, quenching is applied to stop the cellular metabolism instantly. For yeasts, cold methanol quenching is accepted to be the most suitable method to stop metabolism, while keeping the cells intact for separation from the supernatant. During this treatment, metabolite loss may occur while the cells are suspended in the quenching solution...
March 15, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28334169/characterization-of-saccharomyces-uvarum-beijerinck-1898-and-related-hybrids-assessment-of-molecular-markers-that-predict-the-parent-and-hybrid-genomes-and-a-proposal-to-name-yeast-hybrids
#14
Huu-Vang Nguyen, Teun Boekhout
The use of the nuclear-DNA re-association technique has led taxonomists to consider Saccharomyces uvarum a synonym of S. bayanus. The latter, however, is not a species but a hybrid harbouring S. eubayanus (Seu) and S. uvarum (Su) sub-genomes with a minor DNA contribution from S. cerevisiae (Sc). To recognize genetically pure lines of S. uvarum and putative interspecies hybrids among so-called S. bayanus strains present in public culture collections, we propose the use of four markers that were defined from the S...
March 3, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28334164/transcription-factor-mxr1-promotes-the-expression-of-aox1-by-repressing-glycerol-transporter-1-in-pichia-pastoris
#15
Chunjun Zhan, Yankun Yang, Zhenyang Zhang, Xiang Li, Xiuxia Liu, Zhonghu Bai
In methylotrophic yeast Pichia pastoris (P. pastoris), the efficient promoter of alcohol oxidase (PAox1) is induced by methanol and repressed by glycerol, but the molecular mechanism is not clear. In this study, the relationship between alcohol oxidase 1 (aox1), methanol expression regulator 1 (mxr1) and glycerol transporter 1 (gt1) was studied. By RT-PCR, it was found that the overexpression of gt1 could increase the glycerol content in cells and repress the expression of mxr1 and aox1, and the deletion of gt1 reduced the glycerol content in cells and promoted the expression of aox1 ...
March 3, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28334124/a-newly-identified-amino-acid-substitution-t123i-in-the-14%C3%AE-demethylase-erg11p-of-candida-albicans-confers-azole-resistance
#16
YongQin Wu, Ning Gao, Cui Li, Jing Gao, ChunMei Ying
The increasing prevalence of azole resistance in Candida albicans poses a growing problem for clinical treatment. Amino acid substitution of the 14α-demethylase (Erg11p) encoded by the ERG11 gene is one of the most common mechanisms involved in azole resistance. Although amino acid substitutions of Erg11p have been observed in many clinical isolates, only a few amino acid substitutions have been confirmed to be related to azole resistance. In this study, by amplifying and sequencing the open reading frame of the ERG11 gene from 55 clinical isolates, we identified 27 fluconazole-resistant isolates that harbor a novel amino acid substitution, T123I, in Erg11p, in addition to the previously described homozygous substitution Y132H...
February 25, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28334119/climbing-the-yeast-cell-wall
#17
Enrico Cabib
Here is a life in three countries, with different cultures, different political structures and even different skies. The constant through all these changes is the addiction of the subject of this story to science and laboratory work. Perhaps the tale that unfolds here will show to some beginners in research that persistence, seasoned with a little luck, can bring results and satisfaction in the long run.
February 25, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28475761/sugar-versus-fat-elimination-of-glycogen-storage-improves-lipid-accumulation-in-yarrowia-lipolytica
#18
Govindprasad Bhutada, Martin Kavšcek, Rodrigo Ledesma-Amaro, Stéphane Thomas, Gerald N Rechberger, Jean-Marc Nicaud, Klaus Natter
Triacylglycerol (TAG) and glycogen are the two major metabolites for carbon storage in most eukaryotic organisms. We investigated the glycogen metabolism of the oleaginous Yarrowia lipolytica and found that this yeast accumulates up to 16% glycogen in its biomass. Assuming that elimination of glycogen synthesis would result in an improvement of lipid accumulation, we characterized and deleted the single gene coding for glycogen synthase, YlGSY1. The mutant was grown under lipogenic conditions with glucose and glycerol as substrates and we obtained up to 60% improvement in TAG accumulation compared to the wild-type strain...
May 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28444380/polymorphisms-in-the-lac12-gene-explain-lactose-utilisation-variability-in-kluyveromyces-marxianus-strains
#19
Javier A Varela, Noemi Montini, Damhan Scully, Ralph Van der Ploeg, Mislav Oreb, Eckhard Boles, Junya Hirota, Rinji Akada, Hisashi Hoshida, John P Morrissey
Kluyveromyces marxianus is a safe yeast used in the food and biotechnology sectors. One of the important traits that sets it apart from the familiar yeasts, Saccharomyces cerevisiae, is its capacity to grow using lactose as a carbon source. Like in its close relative, Kluyveromyces lactis, this requires lactose transport via a permease and intracellular hydrolysis of the disaccharide. Given the importance of the trait, it was intriguing that most, but not all, strains of K. marxianus are reported to consume lactose efficiently...
May 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28419220/genome-sequence-and-physiological-analysis-of-yamadazyma-laniorum-f-a-sp-nov-and-a-reevaluation-of-the-apocryphal-xylose-fermentation-of-its-sister-species-candida-tenuis
#20
Max A B Haase, Jacek Kominek, Quinn K Langdon, Cletus P Kurtzman, Chris Todd Hittinger
Xylose fermentation is a rare trait that is immensely important to the cellulosic biofuel industry, and Candida tenuis is one of the few yeasts that has been reported with this trait. Here we report the isolation of two strains representing a candidate sister species to C. tenuis. Integrated analysis of genome sequence and physiology suggested the genetic basis of a number of traits, including variation between the novel species and C. tenuis in lactose metabolism due to the loss of genes encoding lactose permease and β-galactosidase in the former...
May 1, 2017: FEMS Yeast Research
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