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FEMS Yeast Research

Chien-Wei Huang, Michelle Walker, Bruno Fedrizzi, Miguel Roncoroni, Richard Gardner, Vladimir Jiranek
The undesirable rotten-egg odour of hydrogen sulfide (H2S) produced by yeast shortly after yeast inoculation of grape musts, might be an important source of desirable varietal thiols, which contribute to tropical aromas in varieties such as Sauvignon Blanc. In this study, we observed that Saccharomyces cerevisiae strains produce an early burst of H2S from cysteine. Both Δmet2 and Δmet17 strains produce a larger burst, likely because they are unable to utilise the H2S in the sulfate assimilation pathway. For the first time, we show that TUM1 is partly responsible for the early production of H2S from cysteine...
December 2, 2016: FEMS Yeast Research
Jun-Ichi Nagao, Tamaki Cho, Makoto Mitarai, Keishi Iohara, Kazumi Hayama, Shigeru Abe, Yoshihiko Tanaka
Protamine peptides (PP) derived from salmon are 14-mer consisting of 10 arginine residues. We investigated the in vitro and in vivo antifungal activity of PP against Candida albicans PP showed the concentration-dependent dual mode of action; fungicidal activity and inhibitory activity of hyphal development in vitro. At lethal concentration of PP, intracellular accumulation of PP was energy-dependent but independent of endocytosis that resulted in ATP efflux and the generation of reactive oxygen species in the cells...
December 2, 2016: FEMS Yeast Research
Benjamin Pardo, Laure Crabbé, Philippe Pasero
Eukaryotic cells activate the S-phase checkpoint in response to a variety of events affecting the progression of replication forks, collectively referred to as replication stress. This signaling pathway is divided in two branches: the DNA damage checkpoint (DDC) and the DNA replication checkpoint (DRC). Both pathways are activated by the sensor kinase Mec1 and converge on the effector kinase Rad53. However, the DDC operates throughout the cell cycle and depends on the checkpoint mediator Rad9 to activate Rad53, whereas the DRC is specific to S phase and is mediated by Mrc1 and other fork components to signal replication impediments...
December 2, 2016: FEMS Yeast Research
Genrikh V Serpionov, Alexander I Alexandrov, Michael D Ter-Avanesyan
Expansion of polyglutamine stretches in several proteins cause neurodegenerative amyloidoses, including Huntington disease. In yeast, mutant huntingtin (mHtt) with a stretch of 103 glutamine residues (HttQ103) forms toxic aggregates. A range of yeast strains have been used to elucidate the mechanisms of mHtt toxicity, and have revealed perturbations of various unrelated processes. HttQ103 aggregates can induce aggregation of cellular proteins, many of which contain glutamine/asparagine-rich regions, including Sup35 and Def1...
December 2, 2016: FEMS Yeast Research
Dmitry S Karpov, Evgenia N Grineva, Arvo T Leinsoo, Nonna I Nadolinskaia, Nataliya K Danilenko, Vera V Tutyaeva, Daria S Spasskaya, Olga V Preobrazhenskaya, Yuriy P Lysov, Vadim L Karpov
The transcription factor ScRpn4 coordinates the expression of Saccharomyces cerevisiae proteasomal genes. ScRpn4 orthologs are found in a number of other Saccharomycetes yeasts. Their functions, however, have not yet been characterised experimentally in vivo. We expressed the Debaryomyces hansenii DEHA2D12848 gene encoding an ScRpn4 ortholog (DhRpn4), in an S. cerevisiae strain lacking RPN4 We showed that DhRpn4 activates transcription of proteasomal genes using ScRpn4 binding site and provides resistance to various stresses...
November 16, 2016: FEMS Yeast Research
Teun Boekhout, Cletus P Kurtzman
No abstract text is available yet for this article.
November 3, 2016: FEMS Yeast Research
Catherine H Freudenreich, Xiaofeng A Su
Early screens in yeast for mutations exhibiting sensitivity to DNA damage identified nuclear pore components, but their role in DNA repair was not well understood. Over the last decade, studies have revealed that several types of persistent DNA lesions relocate to either the nuclear pore complex (NPC) or nuclear envelope. Of these two sites, the nuclear pore appears to be crucial for DNA repair of persistent double-strand breaks (DSBs), eroded telomeres, and sites of fork collapse at expanded CAG repeats. Using a combination of cell biological imaging techniques and yeast genetic assays for DNA repair, researchers have begun to understand both the how and why of lesion relocation to the NPC...
October 30, 2016: FEMS Yeast Research
Yu Chen, Jens Nielsen
Protein phosphorylation is one of the most important mechanisms regulating metabolism as it can directly modify metabolic enzymes by the addition of phosphate groups. Attributed to such a rapid and reversible mechanism, cells can adjust metabolism rapidly in response to temporal changes. The yeast Saccharomyces cerevisiae, a widely used cell factory and model organism, is reported to show frequent phosphorylation events in metabolism. Studying protein phosphorylation in S. cerevisiae allow for gaining new insight into the function of regulatory networks, which may enable improved metabolic engineering as well as identify mechanisms underlying human metabolic diseases...
October 25, 2016: FEMS Yeast Research
Stefan Hohmann
No abstract text is available yet for this article.
October 20, 2016: FEMS Yeast Research
Charles A Abbas
No abstract text is available yet for this article.
October 17, 2016: FEMS Yeast Research
Velimir Gayevskiy, Soon Lee, Matthew R Goddard
Humans have acted as vectors for species and expanded their ranges since at least the dawn of agriculture. While relatively well characterized for macrofauna and macroflora, the extent and dynamics of human-aided microbial dispersal is poorly described. We studied the role which humans have played in manipulating the distribution of Saccharomyces cerevisiae, one of the world's most important microbes, using whole genome sequencing. We include 52 strains representative of the diversity in New Zealand to the global set of genomes for this species...
October 15, 2016: FEMS Yeast Research
Eziuche A Ugbogu, Ke Wang, Lilian M Schweizer, Michael Schweizer
Two of the five unlinked genes theoretically capable of encoding 5-phosphoribosyl-1(α)-pyrophosphate (PRPP) synthetase (Prs) in Saccharomyces cerevisiae, PRS1 and PRS5, contain in-frame insertions which separate the cation- and PRPP-binding sites, diagnostic of Prs polypeptides. The impairment of cell wall integrity (CWI) mitogen-activated protein kinase (MAPK) cascade in strains lacking PRS1 and the synthetic lethality associated with loss of PRS1 and PRS5 imply that these insertions are not gratuitous. Co-immunoprecipitation revealed that Prs1 interacts with the CWI MAPK pathway, only when Slt2 has been phosphorylated by Mkk1/2...
October 15, 2016: FEMS Yeast Research
Peng Mao, John J Wyrick
DNA repair is critical to maintain genome stability. In eukaryotic cells, DNA repair is complicated by the packaging of the DNA 'substrate' into chromatin. DNA repair pathways utilize different mechanisms to overcome the barrier presented by chromatin to efficiently locate and remove DNA lesions in the genome. DNA excision repair pathways are responsible for repairing a majority of DNA lesions arising in the genome. Excision repair pathways include nucleotide excision repair (NER) and base excision repair (BER), which repair bulky and non-bulky DNA lesions, respectively...
October 12, 2016: FEMS Yeast Research
Arpita Maity, Anusha Chaudhuri, Biswadip Das
In Saccharomyces cerevisiae, nuclear exosome along with TRAMP and DRN selectively eliminate diverse aberrant messages. These decay apparatus appear to operate as independent mechanisms in the nucleus. Here, using genetic and molecular approach we systematically investigate the functional relationship between exosome, TRAMP, and DRN mechanisms by examining their relative contributions in the degradation of diverse classes of aberrant nuclear mRNAs generated at various phases of mRNP biogenesis. Our findings suggest that nuclear exosome in association with the TRAMP complex exclusively degrades the transcription-assembly defective mRNPs and splice-defective intron-containing pre-mRNAs, whereas nuclear exosome along with DRN solely degrades the export-defective messages...
September 29, 2016: FEMS Yeast Research
Marie-Noëlle Simon, Dmitri Churikov, Vincent Géli
Replicative senescence is triggered by short unprotected telomeres that arise in the absence of telomerase. In addition, telomeres are known as difficult regions to replicate due to their repetitive G-rich sequence prone to secondary structures and tightly bound non-histone proteins. Here we review accumulating evidence that telomerase inactivation in yeast immediately unmasks the problems associated with replication stress at telomeres. Early after telomerase inactivation, yeast cells undergo successive rounds of stochastic DNA damages and become dependent on recombination for viability long before the bulk of telomeres are getting critically short...
September 27, 2016: FEMS Yeast Research
Martin Kupiec
Each time a cell duplicates, the whole genome must be accurately copied and distributed. The enormous amount of DNA in eukaryotic cells requires a high level of coordination between polymerases and other DNA and chromatin-interacting proteins to ensure timely and accurate DNA replication and chromatin formation. PCNA forms a ring that encircles the DNA. It serves as a processivity factor for DNA polymerases, and as a landing platform for different proteins that interact with DNA and chromatin. It thus serves as a signaling hub and influences the rate and accuracy of DNA replication, the re-formation of chromatin in the wake of the moving fork, and the proper segregation of the sister chromatids...
September 24, 2016: FEMS Yeast Research
Moshe Kafri, Eyal Metzl-Raz, Felix Jonas, Naama Barkai
The minimal description of a growing cell consists of self-replicating ribosomes translating the cellular proteome. While neglecting all other cellular components, this model provides key insights into the control and limitations of growth rate. It shows, for example, that growth rate is maximized when ribosomes work at full capacity, explains the linear relation between growth rate and the ribosome fraction of the proteome, and defines the maximal possible growth rate. This ribosome-centered model also highlights the challenge of coordinating cell growth with related processes such as cell division or nutrient production...
September 19, 2016: FEMS Yeast Research
Zhongpeng Guo, Lisbeth Olsson
High initial cell density is used to increase volumetric productivity and shorten production time in lignocellulosic hydrolysate fermentation. Comparison of physiological parameters in high initial cell density cultivation of Saccharomyces cerevisiae in the presence of acetic, formic, levulinic and cinnamic acid demonstrated general and acid-specific response of cells. All the acids studied impaired growth and inhibited glycolytic flux, and caused oxidative stress and accumulation of trehalose. However, trehalose may play a role other than protecting yeast cells from acid-induced oxidative stress...
September 11, 2016: FEMS Yeast Research
Tatiana A Defosse, Céline Melin, Marc Clastre, Sébastien Besseau, Arnaud Lanoue, Gaëlle Glévarec, Audrey Oudin, Thomas Dugé de Bernonville, Patrick Vandeputte, Tomas Linder, Jean-Philippe Bouchara, Vincent Courdavault, Nathalie Giglioli-Guivarc'h, Nicolas Papon
The fungal CTG clade includes a number of well-known yeasts that impact human health or with high biotechnological potential. To further extend the set of molecular tools dedicated to these microorganisms, the initial focus of this study was to develop a mycophenolic acid (MPA) resistance cassette. Surprisingly, while we were carrying out preliminary susceptibility testing experiments in a set of yeast species, Meyerozyma guilliermondii, while not being a MPA producer, was found to be primarily resistant towards this drug, whereas a series of nine related species were susceptible to MPA...
September 11, 2016: FEMS Yeast Research
Massimo Cogliati, Roberta D'Amicis, Alberto Zani, Maria Teresa Montagna, Giuseppina Caggiano, Osvalda De Giglio, Stella Balbino, Antonella De Donno, Francesca Serio, Serdar Susever, Cagri Ergin, Aristea Velegraki, Mohamed S Ellabib, Simona Nardoni, Cristina Macci, Salvatore Oliveri, Laura Trovato, Ludovico Dipineto, Volker Rickerts, Ilka McCormick-Smith, Sevim Akcaglar, Okan Tore, Emilija Mlinaric-Missoni, Sebastien Bertout, Michele Mallié, Maria da Luz Martins, Ana C F Vencà, Maria L Vieira, Ana C Sampaio, Cheila Pereira, Giuseppe Criseo, Orazio Romeo, Stéphane Ranque, Mohammed H Y Al-Yasiri, Meltem Kaya, Nilgun Cerikcioglu, Anna Marchese, Luigi Vezzulli, Macit Ilkit, Marie Desnos-Ollivier, Vincenzo Pasquale, Maya Korem, Itzhack Polacheck, Antonio Scopa, Wieland Meyer, Kennio Ferreira-Paim, Ferry Hagen, Bart Theelen, Teun Boekhout, Shawn R Lockhart, Kathrin Tintelnot, Anna Maria Tortorano, Françoise Dromer, Ashok Varma, Kyung J Kwon-Chung, Joäo Inácio, Beatriz Alonso, Maria F Colom
No abstract text is available yet for this article.
November 2016: FEMS Yeast Research
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