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Zhongguo Shi Yan Xue Ye Xue za Zhi

Chen Tian, Yue-Yang Li, Dong-Zhi Hu
Although the application of combined chemotherapy has made a great progress in treatment of adult acute myeloid leukemia (AML), the overall survival rate is still not high, mainly because of high recurrence rate and drug resistance. The treatment regimen for relapsed/refractory AML is always strong. Due to the chemotherapy drugs lacking of specific identification of tumor cells, significant adverse reactions often come out and sometimes even endanger the life of patients. In order to improve the life quality and survival rate, the new treatment strategy is urgently needed...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yan-Bin Pang, Ping Wu, Luo-Ming Hua, Xin DU, Jing Wang
The myelodysplastic syndromes (MDS) are a heterogeneous group of clonal myeloid disorders characterized by ineffective hematopoiesis and increased risk of transformation to acute myelogenous leukemia (AML). The treatment of MDS is highly dependent on the reliability of the prognostic evaluation model. Current clinical prognostic scoring systems are comprised of morphology, pivotal clinical trials and cytogenetic findings. However, none of the available prognostic systems incorporates disease-related molecular abnormalities, such as somatic mutations...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Kun Chi, Xiang-Yu Jing
Cytogenetic abnormalities get wide attention for its guidance value for prognosis and therapy in myelodysplastic syndrome (MDS) and related malignancies. Cytogenetic analysis is also the key to clarify the molecular pathogenesis of these kinds of diseases. The traditional karyotyping technique including metaphase cytogenetic (MC) karyotype analysis and immune fluorescence in situ hybridization (FISH) can detect the chromosomal abnormalities to some degree while the positive rate detected by the techniques is low due to the low resolution, dependence on metaphase dividing cells or the limitation of specific sites on the chromosomes, respectively...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Wen-Jing Fan, Zhi-Qiao Fan, Mei-Juan Yang, Yao-Zhu Pan, Hai Bai
Cereblon(CRBN) is a brain-associated protein with ionic protease activity, which interacts with DNA damage-binding protein-1 (DDB1), Cullin 4 (Cul4A or Cul4B), and regulator of Cullins 1 (RoC1) to form the functional E3 ubiquitin ligase complex(CRBN-CRL4) that performs proteolysis via the ubiquitin-proteasome pathway. And CRBN is a necessary target protein for the anti-myeloma effect of immunomodulators. The combination of lenalidomide and CRBN recruited a new substrate that binds to the CRBN-CRL4 complex, leading to increased ubiquitination and proteasome-dependent degradation, thus resulting in anti-myeloma activity...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jing Shi, Tian-Tian Ma, Hua-Sheng Liu
In recent years, with the incidence of malignant tumors increasing year by year, its treatment has been made great progress on the basis of traditional treatments such as surgery, radiotherapy and chemotherapy, it mainly focuses on rapid development of cellular immunotherapy. The efficacy of dendritic cells combined with cytokine induced killer cell (DC-CIK) immunotherapy for cancer patients is positive, it shows good antitumor activities and the abilities to reconstruct and enhance the immune system of tumor patients in clinical application, it has potential application value...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xi Jia, Yang He, Chang-Geng Ruan
Thrombotic thrombocytopenic purpura (TTP) is an acute, potentially life-threatening thrombotic microangiopathies (TMA). TTP has mainly been diagnosed by clinical findings, such as thrombocytopenia and microangiopathic haemolytic anemia. In addition, the reduced activity of von Willebrand factor-cleaving metalloproteinase ADAMTS13 below 10% has been accepted internationally as a diagnostic criterion for TTP. In clinic, the accurate diagnosis and early initiation of therapy can significantly improve the survival rate of patients...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Wen-Jing Fan, Tao Wu, Hai Bai
POEMS syndrome is a rare multiple organ involvement of the parasympathetic syndrome associated with abnormal plasma cells, mostly with high-dose chemotherapy and stem cell transplantation for the treatment. Recently, more treatment attempts to treat POEMS syndrome have been utilized so as to improve the efficacy and safety for the patients with POEMS syndrome, such as immunomodulator, alkylating agent, cytokine-induced killer cells and so on. Lenalidomide has a significant effect on relapse/refractory POEMS syndrome and patients with endocrinopathy...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yue-Yang Li, Dong-Zhi Hu, Chen Tian
The 2016 world health organization (WHO) classification of B cell chronic lymphoproliferative disease (B-CLPD) includes chronic lymphocytic leukemia (CLL), B prolymphocytic leukemia, (B-PLL), hairy cell leukemia (HCL), marginal zone lymphoma (MZL), follicular lymphoma (FL), mantle cell lymphoma (MCL), Waldenstrom macroglobulinemia (LPL/WM). All the above-mentioned diseases are partially similar in cell morphology, immunophenotype and molecular genetics, but significantly different in treatment and prognosis...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Min-Er Xie, Fang Dong, Tao Cheng, Ema Hideo
Hematopoietic stem cells (HSC) maintain homeostatic hematopoiesis via their multi-lineage differentiation and self-renewal potentials. HSC can be enriched and purified by flow cytometry relying on their cell surface markers and functional characteristics, however, these methods can not meet the need for deep analysis of HSC biological property and function because of the poor purity. Recent studies have successfully purified and tracked HSC using specifically expressed genes, which can enhance the purification efficiency of HSC, thus provide a better tool for the in-vivo study of HSC...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Shi-Yong Deng, Ming Chen, Xu-Dong Wang, Xia Guo, Kun Wu, Xia Zhang
OBJECTIVE: To investigate the diagnostic value of combined detection of HLA-G and IL-6 in children with infectious mononucleosis (IM). METHODS: Eighty-three children suffered from infectious mononucleosis hospitalized in Wuhan Children's Hospital(Wuhan Maternal and Child Healthcare Hospital) from January 2014 to June 2017 were selected as the IM group, 83 healthy children in the same period were selected as the as control group. Enzyme-linked in munosorbent assay (ELISA) was used to detect and compare the changes of HLA-G and IL-6 levels between 2 groups...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xin-Fang Zhu, Yu-Bo Wu, Li-Hui Yang, Rong Xia, Jing-Han Liu, Jiong-Cai Lan
OBJECTIVE: Through researching preoperative coagulation function in the case of ABO-identical blood insufficient for emergency rescue transfusion according to recommended programs of special emergency rescue transfusion was carried out, the relationship between volume of blood products and coagulation function was analyzed. METHODS: The surgical cases of blood transfusion more than 1 600 ml during operation were collected in our hospitals from Aug 2015 to Dec 2016(n=218), these cases were divided into the normal coagulation group(Group A) and abnormal coagulation group(Group B), and the patients of emergency rescue transfusion O type blood group(Group C)...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Rong Fu, Jin Yu, Ming-Zhen Jing, Xu-Ying Wang
OBJECTIVE: To investigate the infection of HHV-8 virus in free blood donors in Wuhan. METHODS: Whole blood samples were collected from 450 free blood donors in Wuhan, and the genomic DNA extraction and serum collection were performed respectively. And then, the positive rate of HHV-8 was detected by PCR and ELISA, the positive detection rates were compared between them. Finally, HHV-8 ORFK1 gene was cloned by PCR in the positive samples, and the HHV-8 ORFK1 gene th was genetyped through sequencing analysis, homology comparison and phylogenetic tree construction...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jiao-Jiao Yin, Bing Li, Qiang Zhao, Shu-Ting Zhang, Yao-Zhu Pan, Hai Bai
OBJECTIVE: To investigate the autophagy activity changes of umbilical cord mesenchymal cells (MSC) under hypobaric hypoxia and the effect of hypobaric hypoxia on cell viability. METHODS: Umbilical cord mesenchymal cells were cultured in the chamber of hypobaric hypoxia with an air pressure of 41.1 kPa and an oxygen density of 1%. At 0, 4, 8, 16, 24 and 48 hours, the cells were harvested for Western blot and real-time PCR to observe the expression level of the autophagy marker protein LC3B...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Zi-Cen Feng, Yu-Qi Wen, Meng-Ge Wang, Qian Tu, Hong-Tao Wang, Zheng-Yu Wang, Jia-Xi Zhou
OBJECTIVE: To explore the role of bromodomain and extra terminal (BET) bromodomain in hematopoietic differentiation from human enbryonic stem cells (hESC). METHODS: The effect of BET hematopoietic inhibitor I-BET151 on hematopoietic differentiation from hESC was detected by using a monolayer hematopoietic defferentiation model, immunofluorescence, flow cytometry and real-time PCR; moreover the role of I-BET151 in process of hematopoietic differentiation was explored by adding I-BET151 in different differentiation stages...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Wei Liu, Bing Liu
OBJECTIVE: To establish a transplantation system for a trace amount of adult mouse bone marrow hematopoietic stem cells (HSCs). METHODS: The HSCs from bone marrow cells of GFP adult mouse were seperated by using marker combination ESLAM (CD45+ CD201+ CD150+ CD48- ) The goal cell population acquired by flow cytometry sorting were injected into lethally irradiated recipient mouse plus protective cells. And the peripheral blood cells from recipient mice tail veins at different time points were acquired to observe the reconstruction level and lineage differentiation...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Di Yu, Ya-Hui Han, Chen-Yuan Hu, Bin Zhang, Huan-Xin Zhang, Hu-Jun Li, Kun-Ming Qi, Jiang Cao, Zhen-Yu Li, Zhi-Ling Yan, Kai-Lin Xu
OBJECTIVE: To analyze the risk factors and prognosis of hepatic chronic GVHD after allogeneic hematopoietic stem cell transplantation (allo-HSCT). METHODS: The clinical data of 147 patients undergoing allo-HSCT from January 2013 to December 2016 were analyzed, the correlation between recipient age and sex, disease state, matched degree of HLA, donor sex, stem cell sources, ATG in GVHD prophylaxis, liver dysfunction during conditioning period, pre-transplant HBsAg, prior aGVHD and hepatic cGVHD were studied, and the correlation between hepatic cGVHD and prognosis were analysed...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Lan-Lan Deng, Kun-Yin Qiu, Xiong-Yu Liao, Yi-Wen Liang, Ke Huang, Yang Li, Hong-Gui Xu, Jian-Pei Fang, Dun-Hua Zhou
OBJECTIVE: To prospectively study the correlation BKV with the occurrence and development of late onset hemorrhagic cystitis (LOHC) after allogeneic hematopoietic stem cell transplantation(allo-HSCT). METHODS: The clinical data of a total of 276 patients with allo-HSCT in our department between January 1998 and March 2016 were analyzed ratrospectvely. Quantitative Real-time PCR assay was used to prospectively monitor the BKV DNA load of the urine and plasma for 23 patients accepting allo-HSCT from August 2015 to March 2016...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ying Zhang, Ying Chu, Yong-Hua Yao, Wen-Juan Ye, Yu-Qin Shen, Lian-Hong Xu
OBJECTIVE: To detect the expression level of cyclooxygenase-1(COX-1) and cyclooxygenase-2(COX-2) in the platelet of iron deficiency anemia(IDA)women at childbearing age and to explore its correlation with the different indexes of anemia and platelets. METHODS: Forty female IDA patients at childbearing age and 35 healthy controls were enrolled in this study. The Flow cytometry was used to detect the expression of platelet COX-1 and COX-2,the platelet aggregation function as examined by turbidimetric method,and the levels of serum ferritin were analyzed by electrochemical luminescence method,the leval of serum iron was determined by ELISA,and the correlation of different indexes was analyzed...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xue-Li Wang, Cui-Wei Ouyang
OBJECTIVE: To investigate the changes of cerebral metabolism in rabbit model of hemorrhagic shock by using proton magnetic resonance spectroscopy(PMRS). METHODS: Ten New Zealand white rabbits were used for construction of the model of acute hemorrhagic anemia. 1H-MRS was performed before and at the time-peint of 30, 90, and 180 min after hemorrhagic shock. The concentrations of NAA, Cr, Cho, Lac, and NAA/Cr and Cho/Cr ratios were estimated. RESULTS: Hemorrhagic shock was associated with significant reductions in red blood cell count, hemoglobin level, hematocrit, pH, and PaCO2 , and elevations of blood lactate and PaO2 ...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yao-Wei Liu, Wen Qu, Hua-Quani Wang, Li-Min Xing, Yu-Hong Wu, Zhao-Yun Liu, Yang Zhang, Hui Liu, Xi-Feng Don, Jing-Lian Tao, Zong-Hong Shao
OBJECTIVE: To analyze the number of myeloid-derived suppressor cells(MDSC) and the level of prostaglandin E2(PGE2) in the bone marrow of adult ITP patients, and to explore their possible mechanisms involved in the pathogenesis of this disease. METHODS: Twenty-five patients of newly diagnosed ITP, 25 patients of complete remission group and 15 patients of control group were selected. The number of MDSC in the bone marrow between 3 groups was detect by flow cytometry (FCM)...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
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