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Zhongguo Shi Yan Xue Ye Xue za Zhi

Liu-Yan Xin, Ai-Fei Liu, Si-Si Zhong, Yi-Jian Chen
CRISPR/Cas genome editing technology is a newly developed powerful tool for genetic manipulation, which can be used to manipulate the genome at specific locations precisely, to restore the function of genetic defect cells, and to develop various disease models. In recentl years, with the advances of precise genome manipulation, CRISPR/Cas technology has been applied to many aspects of diseases research and becomes an unique tool to investigate gene function and discover new therapeutic targets for genetic diseases...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Hai-Ying Zhang, Qiang Zhao, Tao Wu
Graft versus host disease (GVHD) is the major complication of allogenic hematopoietic stem cell transplantation (allo-HSCT), and is a mainly responsible result for the non-recurrence-related mortality of long-term survived patient after transplantation. Studies indicated that regulatory T (Treg) cells can inhibit early-GVHD. Since the transcriptional factor NF-κB has been implicated in the regulation of Treg cell proliferation and activation, it becomes a potential target of GVHD therapy. Thus, in this review the function and signaling pathway of NF-κB, as well as the relationship between GVHD and NF-κB, are discussed and summarized...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ya-Ling Zhao, Gui-Min Liu, Li-Jun Zhang, Wen-Tong Liang, Zhi-Yong Cheng
Jauns kinase (JAK)/transducer and activator of transcription(STAT) pathway is a classical approach to study the rapid changes of the gene expression in specific target cells by a variety of extracellular signals. The JAK and STAT transfer cytokine receptor signaling plays a unique role in multiple cellular and molecular biological changes.The abnormal signal of JAK/STAT pathway will lead to the hematopoietic abnormalities.Studies had shown that the abnormal activation of JAK2/STAT signaling pathway are in many kinds of malignant hematological diseases, such as in acute lymphoblastic/myeloid leukemia, chronic myeloid leukemia, lymphoma, myelodysplastic syndromes, myeloprofilerative neoplasm, especially in the patients of myeloproliferative neoplasm(MPN) with JAK gene mutation(JAK2V617F), this mutation has an important value for MPN diagnosis...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Li-Xiao Shi, Jian-Hua Wang, Xiao-Dong Shi
The PI3K/Akt/mTOR signaling pathway plays a central role in cell growth, proliferation and survival in physiological conditions. This signal pathway is considered to be an innovative targeted therapy of cancer, and its abnormal activation has been proved to be related to T-cell acute lymphoblastic leukemia (T-ALL) .Despite improved treatment strategies, such as multi-drug combination, high-dose chemotherapy and all kinds of application and popularization of hematopoietic stem cell transplantation, children with drug resistance or relapse T-ALL are still rather worse and its overall outcome and prognosis are much poorer than the more common B-lineage ALL...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Min Wang, Li-Ru Wang
Ki-67 antigen expression is closely associated with the cell cycle. Its expression is in almost the entire mitosis in cell cycle. Detection of Ki-67 antigen can reflect the cell proliferation activity in hematological malignancies. Detection of Ki-67 has been widely applied in staging, therapy effect monitoring and prognosis judgment in lymphoma. There has been a lot of studies investigating the expression of Ki-67 in acute lymphocytic leukemia (ALL) and chronic lymphocytic leukemia (CLL), and investigating the relevance between Ki-67 expression and therapy effect and prognosis...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jing-Zheng Zhang, Jian Gu
Inflammasome is a complex of protein, which can recognize pathogen molecules and pathogens simulating human heat shock protein via pattern recognition receptors, thus it causes their own activation, sensitizes NF-κB pathway, induces the production of inflammatory mediators, leads the chronic inflammation and promotes thrombosis. Some studies reported that nearly 17% of the new tumors can be caused by chronic inflammation, and it seems that there is a complicated relationship between inflammasome and cancer-associated thrombosis...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jing-Shi Wang, Zhao Wang
Erdheim-Chester Disease (ECD) is a rare form of non Langerhans' cell histiocytosis. The etiology of ECD is unknown yet. The disease is characterized by the infiltration of lipid-laden histiocytes with foamy to bones and various organs. The heterogeneous manifestations of ECD vary among different individuals. This results in a presentation that may vary from an indolent focal disease to a life threatening organ failure. This review focuses on the etiology, clinical presentation, diagnostic criteria and treatment of Erdheim-Chester disease (ECD)...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jun Yuan, Hong-Ling Hao, Yan Li, Rui-Cang Wang, Xiao-Xia Zhang
There is no gold diagnostic standard for BCR-ABL fusion gene negative chronic myeloproliterative neoplasm(cMPN). The following detection methods such as comprehensive bone marrow cell morphology, bone marrow pathology, genetic mutation, flow cytometry and immunohistochemical are needed to diagnose the BCR-ABL fusion gene positive cMPN. The JAK2 mutation can be used as a specific diagnostic criteria for polycythemia vera (PV), but there is no specific and sensitive indication for the JAK2 mutation-negative MPN...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
He Huang, Wen-Jian Guo, Ron-Xin Yao
Multiple myeloma(MM) as one of the most common tumors of hmatologic system, is characterized by malignant proliferation of plasma cells, and the chemotherapy is the main therapeutic method. MM is an incurable disease because of drug-resistance of MM cells. Although the pathogenesis of MM remains unknown, the chromosome abnormalities exit in half of the patients, particularly the highly expressed gene C-MYC. Furthermore, plenty of clinical researches indicated a high expression level of C-MYC implied worse progression and/or poor prognosis of MM...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ming Liang, Hui Zhang, Fu-Xiong Chen
Hematologic malignancies are the most common childhood malignancy and the main cause of death in pediatric cancer patients. Systemic diagnosis and risk-based treatment in these 30 years have achived a great improvement in pediatric cancer therapy, for example, the event-free 5-years survival rate of childhood acute lymphoblastic leukemia was over 80%. Unfortunately, 20%-25% patients relapsed after-therapy, therefore, the novel therapeutic strategies that can improve survival are urgently required. T lymphocytes are the most potent effective anti-tumor cells...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yan-Fen Li, Hai-Yan Zhu
Outstanding progress has been achieved in the treatment of Hodgkin's lymphoma in recent years, most of patients can be cured by using modern therapy. However, more and more attentions have been paid on treatment-related complications, the current treatment strategies are focusing on further improving treatment efficacy, while reducing treatment-related toxicity. It is still to be determined whether interim PET imaging could directly judge the therapeutic response so as to make the individualized treatment programs for the patients, for instance through intensifying or abbreviating chemotherapy regimens and/or omitting radiotherapy...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Bing-Jie Wang, Xi-Nan Cen, Han-Yun Ren
Histologic transformation (HT) is a frequent event in the clinical course of patients with indolent lymphoma with dismal outcome. The diagnosis of HT is based on clinical manifestation, PET-CT and pathologic biopsy, and the latter is a golden standard for HT. There are contradictory data about the impact of initial management on the risk of transformation. Patients who present with HT did not receive R-CHOP or chemotherapy-naive, should receive this regimen. For the subset of patients received R-CHOP prior to HT, the second line chemotherapy for DLBCL should be adopted...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Guo-Liang Ding, Wei-Sheng Qin, Lin-Yuan Zhao, Lin Zhu, Yu-Fang Bo, Zhen Liu, Jing-Han Liu
OBJECTIVE: To explore the key technique for preparation of the frozen platelet and efficacy of its clinical application. METHODS: The influences of the donators' peripheral platelet count, starting time of freeze, injection rate and evenness of the freeze-protective agent, storage mode, re-melting temperature and the capacity of water-bath etc. on the quality of the frozen platelets were analyzed retrospectively in 3 257 samples of frozen platelets before platelet pheresis...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ya-Lan Lu, Xin Zhang, Yu-Fang Wang, Shan-Dong Ke, Jin-Yong Ke, Geng-Fu Liu, Shi-Ming Chen
OBJECTIVE: To study the influence of leukodeplated blood transfusion on cellular immunity of patients with acute leuemia, so as to provide support for application of leuko-deplated blood transfusion in clinic. METHODS: A total of 100 AL patients from January 2012 to December 2015 were chosen, and were divided into 2 groups: leukodeplated blood transfusion group(50 cases) and routine blood transfusion group(RBT) as control (50 cases). The effective rate, side effects, peripheral blood T cells and expression level of TLR2 and TLR4 were compared between 2 groups...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ping Lu, Bing Ling, Ning Wang, Lennart Hammarstrom
UNLABELLED: Objective:To calculate the prevalence of IgAD in a replicate cohort of the Chinese Han population in Shanghai area by screening blood donors and to study the genetic difference of IgAD individuals in the Mongoloid population. METHODS: The prevalence of IgAD in a large number of Chinese blood donors (n=61624) in Shanghai area was investigated. The immunoglobulin class, IgG subclass and anti-IgA serum levels were measured among the IgAD donors. These donors were subsequently tissue typed and the allele frequency was compared with the Shanghai bone marrow donor HLA registry...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ling Ma, Yan-Chun Liu, Ruo-Yang Zhang, Xiao-Yu Zhou, Jing-Ming Xun
OBJECTIVE: To investigate the feasibility of noninvasive fetal ABO genotyping based on RASSF1A gene with circulating cell-free fetal DNA(cffDNA) from maternal plasma. METHODS: DNA was extracted from the O group pregnant plasma, and the presence of cffDNA was confirmed by fetal DNA maker SRY and RASSF1A. B and non-O were detected by real-time PCR, and the genotyping results were evaluated by using the serologic tests for ABO phenotyping. RESULTS: Among the samples of 20 cases, the SRY was found in 11 cases by detecteion, the detection results were consistent with sex of infants after delivery; the RASSF1A was amplified all in samples of other 9 cases after BstU1 cleavage, which confirmed existance of cffDNA...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jing Wang, Chun Zhou, Ni-Zhen Jiang
OBJECTIVE: To understand the epidemiological characteristics of syphilis infection in blood donors of Chinese Nanjing area, to strengthen control of blood quality and to provide the scientific evidence for working out the strategy of prevention and treatment of syphilis infection. METHODS: Firstly the double antigen sandwich ELISA was carried out according to its instructions, test results showing positive will be examed by double orifice second-round. RESULTS: A total of 362425 blood samples were collected from 2010 to 2014, among them 1277 positive samples were detected...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Lin-Lin Hu, Hong Zhen, Xiao-Nan Zhang, Li Zhou, Hesham M Amin, Ping Shi
UNLABELLED: Objective:To explore the effect of tyrosine phosphorylation sites Tyr644 and Tyr664 in oncogenic protein NPM-ALK on cell cycle and its related mechanisms. METHODS: Transiently transfected 293T cells and stably transfected Jurkat cells were used for analysis of cell cycle and protein after the transfection with the constructed recombinant plasmid pEGFP-N1, pEGFP-N1-NPM-ALK and pEGFP-N1-NPM-ALK(644, 664); soft agar assay for colony formation was performed to examine the different carcinogenicity of stable cell lines; cell viability of stable cell lines was examined by CCK-8 after the treatment with PPP...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Fei-Yan Wang, Lan Luo, Sha-Sha Zhao, Hua-Ping Wei, Li Wang, Zhen-Yang Gu, Li-Xun Guan, Nan Yang, Zhe Gao, Li-Li Wang, Chun-Ji Gao
OBJECTIVE: To investigate the expression profile of lncRNA NONHSAT040475 in peripheral blood leukocytes of healthy persons. METHODS: The peripheral blood mononuclear cells were collected from 10 healthy volunteers, the CD3(+) T cells,CD19(+) B cells,CD56(+) NK cells and granulocytes were purified and sorted by flow cytometry. Then, the expression of lncRNA NONHSAT040475 in peripheral blood leukocyte subsets was detected by real-time quantitative PCR. RESULTS: the expression levels of lncRNA NONHSAT040475 were various in lymphocyte subsets, its expression in B lymphocytes was significantly higher, as compared with T lymphocytes, while the expressions of lncRNA NONHSAT040475 in NK cells and granulocytes were relative low(P<0...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jun-Bin Huang, Li Jiang, Chun Chen, Hong-Man Xue
OBJECTIVE: To analyze and compare the clinical features, treatment and prognosis of 31 children with Langerhans cell histiocytosis(LCH) treated with modified DAL-HX83/90 or JLSG-96 protocol. METHODS: The clinical features, treatment and prognosis of 31 children with Langerhans cells admitted in our hospital from January 2005 to December 2014 were analyzed retrospectively. The outcome of patients treated with modified DAL-HX83/90 or JLSG-96 protocols were compared by using the Kaplan-Meier survival curve...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
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