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Zhongguo Shi Yan Xue Ye Xue za Zhi

Shu-Min Qiao, Yi Wang, Xiu-Ling Song, Yi-Nan Yue
Hematopoietic stem cell transplantation is an important method for the treatment of hematological malignancies. The hematopoietic recovery after transplantation, graft-versus-host disease (GVHD) prevention and treatment are the key to affect transplant success. Umbilical cord mesenchymal stem cells (hUC-MSC) can improve the hematopoietic microenvironment, promote hematopoietic stem cell homing and hematopoietic recovery, support hematopoiesis. In aduition, the hUC-MSC have immune function for a variety of immune cells, can avoid and alleviate the host immune response, have a certain effect on the treatment of GVHD...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ai-Fei Liu, Jin-Xia Wang, Fang-Lin Li, Yi-Jian Chen
MicroRNAs (miRNAs) are a class of endogenous non-coding single-stranded small noncoding RNAs with the length of 20 to 23 nucleotides. MicroRNA-125 (miR-125) family, which is a highly conserved miRNA family, is consist of miR-125a, miR-125b-1 and miR-125b-2. Accumulating evidence demonstrated that miR-125 can be involved in various physiological and pathological processes in vivo. Importantly, it is closely related with the tumorigenesis and tumor development, including tumor cell proliferation, apoptosis, invasion and metastasis, metabolism and immune response...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Huan Wang, Jian-Yong Li, Chao Sun, Xin Zhou
Primary plasma cell leukemia (PPCL) is an uncommon and very aggressive hematological disease, which has different cytogenetic abnormalities, special molecular and biological characteristics, compared with multiple myeloma(MM). The clinical course is unfavorable with short remissions and reduced overall survival(OS). Intensive chemotherapy regimens and bortezomib-based regimens, immunoregulatory drugs(IMiDs) as well as stem cell transplantation may be beneficial to PPCL patients. This review summarizes the recent advances of this malignant disease, including clinical and biochemical characteristics of PPCL, therapy and prognosis of PPCL, new drugs for PPCL and so on...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Qian-Chuan Huang, Jin-Ya Ding
More than 80% of patients with MM is present as intact monoclonal immunoglobulin (Ig). Usually, the patients with intact immunoglobulin MM (IIMM) show parallel fluctuations of their intact Ig and FLCs or BJP. In the era of novel agents, including thalidomide, lenalidomide and bortezomib, the natural disease development and classic relapse patterns have been changed, the relapse was characterized by an increase in sFLC or BJP without a corresponding increase in paraprotein level, a phenomenon termed "light chain escape", indicates a worse outcome in patients with MM...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Rui-Meng Zhuang, Jian Cheng
As more and more clinical trials about chimeric antigen receptor T cells (CAR-T) have been carried out successfully, the CAR-T are getting well-known. Thanks for genetic engineering, CAR-T has the tumor special antigen expressed on the surface. CAR-T therapy is the adoptive immunotheraphy for rapidly advancing tumors. This technology has made a great progress in both blood malignancies and solid tumor. However, CAR-T also meet many difficulties in the way of development. This review discusses the clinical application, challenges and developing trend of CAR-T...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jun-Bin Huang, Jian Wang, Li Jiang, Xiao-Jun Wu, Chun Chen, Hong-Gui Xu, Hong-Man Xue
OBJECTIVE: To analyze the clinical features and pathogenetic gene mutation in a fetus at his third trimester with familial haemophagocytic lymphohistiocytosis (FHL). METHODS: Target region sequencing and high-throughput sequencing were used to detect pathogenetic gene mutations for familial haemophagocytic lymphohistiocytosis in a late onset HLH fetus. Pathogenetic gene mutations of the patient and his parents were verified by Sanger dideoxy sequencing. RESULTS: A male neonate, who had right pleural effusion, hepatomegaly and splenomegaly previously revealed by fetus ultrasound, was delivered at full-term by cesarean section...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Qi Chen, Hui Huang, Chang-Jiu Tang, Jing Kong, Lan Zhang
OBJECTIVE: To study the correlation and consistency of the 2 different methods for detecting coagulation function. METHODS: One hundred and six inpatients of ICU and department of gastroenterology in our hospital were retrospectively analyzed. The clinical data of thromboelastography and coagulation test were collected, and the detection results of 2 different methods were statistically analyzed. RESULTS: The K, MA value and α-Angle in thrombelastometry significantly related with fibrinogen (r=-0...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Shou-Shan Men, Lian-Zhi Lv, Yuan-Feng Chen, Chun-Hua Han, Hong-Yu Liu, Yan Yan
OBJECTIVE: To investigate the blood test patterns for blood donors after nucleic acid detection in blood center. METHODS: The collected blood samples after voluntary blood donors first were detected by conventional ELISA, then 31981 negative samples were detected via HBV/HCV/HIV combined nucleic acid test of 6 mixed samples(22716 cases) or single samples(9265 cases) by means of Roche cobas s201 instrument. The combined detection method as follows: the blood samples were assayed by conventional nucleic acid test of 6 mixed samples, at same time, 6 mixed samples were treated with polyethylene glycol precipitation method to concentrate the virus, then the nucleic acid test of blood samples was performed; the single detection method as follows: firstly the conventional nucleic acid test of single sample was performed, then the positive reactive samples after re-examination were 6-fold diluted to simulate the nucleic acid test of 6-mixed samples...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Qian-Ni Liang, Zhi-Jian Liao, Run-Qing Zhang, Yan-Li Ji, Guang-Ping Luo, Mei-Na Li
OBJECTIVE: To performe the immuneserological and RHD Genotype analyses for DVI type 3 genotype pregnemt women with anti-D. METHODS: RhD blood type of this pregnant women was identified by common serological methods, then the blood group specific antibodies was screened and identified; the polymerase chain reaction-sequence specific primer(PCR-SSP) was used to identify the pregnant women's RHD genotype; RhD blood group for the pregnant women, her spouse and daughter was genogrouped and genetically analyzed by multiplex ligation-dependent probe amplification(MLPA)...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Hong Luo, Ji-Zhi Wen, Run-Qing Zhang, Yan-Li Ji, Chun-Yan Mo, Ling Wei, Zhen Wang, Zhi-Jiang Liao, Guang-Ping Luo
OBJECTIVE: To explore the phenotype types and genetic mutation mechanism of Rhesus D variant individuals. METHODS: Fouty-eight peripheral blood samples of pregnancies and blood donors who had been identified as Rhesus D variant by using routine serologic methods were collected from January 2013 to October 2015 in our center. The multiple ligation-dependent probe amplification(MLPA) was used to determine the RHD after genomic DNA had been extracted from the blood sample, then the data including gene copy number variations, point mutations, deletions and hybrid fusions were analyzed by GeneMarker software...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ying Liu, Dong-Mei Bi, Su-Zhen Zhao, Ting-Yu Yan, Feng-Liang Lu, Xin Wang, Peng-Yu Jiang
OBJECTIVE: The purpose of this study was to investigate the 24 kinds of antigen gene polymorphism chara-cteristics in 11 RBC blood group systems of Manchu population blood donor in Harbin Area of China, so as to better perform the clinical blood transfusion and to avoid adverse transfusion reactions. METHODS: Twenty-four antigen genes (GYPB (S/S), Duffy, Kell, Dombrock, Diego, Kidd, Scianna, Colton, Lutheran, Yt, Mur) were assessed by the polymerase chain reaction sequence specific primers (PCR-SSP) in 11 blood type systems, including Fya, Fyb; K, K; Doa, Dob; Dia, Dib; JKa, JKb; Sc1,Sc2; Coa, Cob; Lua, Lub; Yta, Ytb; Kpa, Kpb; Mur, Mur2 among 200 Manchus in Harbin...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Fa-Wen Chen, Hai-Hua Xie, Xiao-Jun Yang, Jia-Feng Sun, Li-Hong Lin, Lu-Fei Chen, Peng-Wei Cai
OBJECTIVE: To explore molecular and genetic mechanism of 3 cases of para-Bombay blood group. METHODS: The bood samples of proband and family members were selected to identify their blood groups with conventional serologic methods, and salivary components carrying the ABH antigens were detected. The coding regions of FUT1 as well as exon 6 and 7 of the ABO gene were amplified using polymerase chain reaction(PCR), and the FUT1 gene was directly sequenced. RESULTS: All the 3 cases of proband were confirmed as para-Bombay blood group...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xie-Bing Bao, Wen-Zhi Cai, Xue-Feng He, Su-Ning Chen, Hui-Ying Qiu, Ai-Ning Sun, De-Pei Wu
OBJECTIVE: To explore the effect of BCR-ABL gene transcripts on Leukemia-free survival (LFS) and prognosis of patients with Philadelphia chromosome positive acute lymphoblastic leukemia (Ph+ ALL) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). METHODS: The clinical data of 107 cases of Ph+ B-ALL patients received allo-HSCT from July 2006 to November 2014 in the First Affiliated Hospital of Soochow University were collected and the relationship between the clinical characteristics and LFS after transplantation was analyzed...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Li Li, Yi Liu, Jia-Xin Liu, De-Feng Zhao, Pei-Hao Zheng, Wen-Jie Yin, Yuan-Yuan Ma, Li-Ren Qian, Heng-Xiang Wang, Chun-Ji Gao, Jian-Liang Shen
OBJECTIVE: To explore the clinical value of detecting adenosine triphosphate (ATP) level in CD4+ T lymphocytes (Immuknow ATP) of patients on early stage after allogeneic hematopoietic stem cell transplantation (allo-HSCT). METHODS: The base-line ATP value in CD4+ T lymphocytes in cases of hematological malignancies and the ATP level in CD4+ T lymphocytes of acute leukemia patients before allo-HSCT were detected. Allo-HSCT recipients were devided into 3 groups with different level of immunereactivity according to ATP concentraiton in month 3 (day 90±5) after allo-HSCT...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yang Fu, Ya-Xiong Jin, Yu-Mei Liu, Qian Niu, Hong Jiang
OBJECTIVE: To evaluate the levels of coagulation indicators [thrombomodulin(TM)/ thrombin-antithrombin complexes(TAT)/ α2-plasmin inhibitor-plasmin complexes(PIC)/ tissue plasminogen activator-inhibitor complexes(t-PAIC) /D-Dimer(D-D)/fibrin degradation products(FDP)] in the critical patients with thromboembolism, analyse their correlation with inflammatory factor (procalcitonin/C reactive protein/ interleukin-6), and explore the diagnostic significance of coagulation indicators for these patients...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yuan Feng, Ling-Ling Geng, Xiao-Qing Li, Nan Nan
OBJECTIVE: To analyze the expression of MicroRNA-15a (miR-15a) in children with primary immune thrombocytopenia (ITP) and its significance. METHODS: The peripheral blood monomuclear cells (PBMNC) were isolated and cultured from ITP patients and healthy volunteers. The expression level of miR-15a was measured by real-time PCR. After miR-15a mimic was transfected into PBMNC, the levels of INF-γ, IL-2, IL-4 and IL-10 were measured by ELISA. RESULTS: The expression of miR-15a was significantly decreased in PBMNC...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Gui-Lan Chen, Yan-Xia Qu, Fan Jiang, Ying Tang, Fang Tang, Lian-Dong Zuo
OBJECTIVE: To investigate the incidence and common types of abnormal hemoglobin diseases of the couples at childbearing age by using high performance liquid chromatography (HPLC) in Guangzhou city. METHODS: The couple of childbearing age in the Birth defect intervention project and Free pre pregnancy health examination were screened from October 2008 to February 2016 in Guangzhou city. The HPLC was used to detect abnormal hemoglobin; The Gap-PCR and reverse dot blot(RDB) were used to detect thalassemia gene deletions...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Wen-Jian Mo, Qian-Qian Sun, Shi-Yi Pan, Ping Mao, Xiao-Wei Chen, Yu-Ping Zhang, Ming Zhou, Yan-Li Xiu, Shun-Qing Wang
OBJECTIVE: To explore the value of CD3+CD4+ T cell count in prediction of viral infections after allogeneic hematopoietic stem cell transplantation(allo-HSCT) in the patients with severe aplastic anemia(SAA). METHODS: A total of 78 SAA patients with allo-HSCT in Guangzhou First People's Hospital from January 2014 to July 2016 were enrolled in this study. The absolute numbers of CD3+CD4+T cells were measured by flow cytometry at 1,2,3,6, and 12 month after allo-HSCT...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ming-Quan Wang, Jing-Jing Gao, Ren-Wei Xie, Qing-Hai Wang
OBJECTIVE: To analyze the prevalence of irregular antibodies in children with severe β-thalassemia after long-term blood transfusion and its correlation with RH and anemia gene mutations site. METHODS: One hundred twenty children with severe β-thalassemia and long-term blood transfusion were selected in our hospital, the genomic DNA was extracted and the genotype of RH factor were determined by PCR-SSP. The irregular antibodies and their types were screened and identified by the serological method, the gene types of the severe β-thalassemia were analyzed by reverse dot blot hybridization on DNA chip and PCR amplification...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Chun-Yang Deng, Shuang Chen, Ming Jiang, Rong Chen, Tuerxun Nilupar, Huan Wang, Jian-Ping Hao
OBJECTIVE: To investigate the value of p15, DAPK, SOCS1 and FHIT genes combined detection in the early diagnosis and prognosis evaluation of patients with myelodysplastic syndrome(MDS). METHODS: The methylation-specific PCR (MSP) was used to detect the methylation of the above-mentioned 4 genes in 67 patients with MDS. The value of 4 gene combined detection in the early diagnosis and prognosis evaluation of patients with MDS was compared and anazlyzed. RESULTS: The methylation rates of p15, DAPK, SOCS1 and FHIT genes in 67 patients with MDS were 37...
December 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
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