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Zhongguo Shi Yan Xue Ye Xue za Zhi

Ya-Zhi Yang, Xiao-Yan Zhang, Jian Li
The replicative senescence of mesenchymal stem cells(MSC) during the in vitro expansion limits their use in the research and treatment of many disease. Also, MSC senescence may promote the individual senility, hypofunction of tissue and organ, and tumorigenesis. Thus, in order to better understand the senescent mechanism and delay the senescence, even reverse MSC senescence, the roles of signaling pathway, such as Wnt/β-catenin, MAPK/ERK, PI3K/AKT and ROS-related signaling pathway during the MSC senescence all were reviewed, so as to can deepen the under-standing of MSC senescence and provide a new thinkings for delaying, even reversing the MSC senescence...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Li Wang, Rui Gao, Shu-Feng Liu, Chun-Ji Gao
Graft-versus-host disease (GVHD) and infection are the frequently encountered complications after hematopoietic stem cell transplantation (HSCT), which influence the outcome and limit the widespread application of HSCT. Intestinal microbiota plays an important role in maintaining intestinal immune balance. The diverse levels of intestinal microbiota associated with the incidences of GVHD, infection and the prognosis of HSCT, thus remodeling intestinal microbiota can alleviate GVHD and infection after HSCT. Herein, the recent research progress about the role and the involved mechanisms of intestinal microbiota in HSCT, and the novel manipulation strategies of intestinal microbiota are systematically reviewed...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xiu-Li Chen, Zhen-Shu Xu
B-cell malignancy, a kind of malignant tumor of blood system, is characterized by heterogeneity and complexity of the pathogenesis. In this review, the recent advances of studies on B-cell malignancy and signaling pathways are briefly summarized. This review focuses on the role of signaling pathways, especially the NF-κ B, Wnt, PI3K/Akt, Notch and JAK/STAT signaling pathway in the occurrence and development of B-cell malignancy, and discussed the influence of signaling pathways on the invasiveness and drug resistance of B-cell malignancy, and the clinical application of signaling pathway inhibitors...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jing Zhou, Yi Xiao
Epstein-Barr virus(EBV) closely relates with the occurrence, development and prognosis of multiple lymphomas, such as Burkitt' s lymphoma, diffuse large B-cell lymphoma, Hodgkin' s lymphoma, angioimmunoblastic T cell lymphoma. The mechanism of EBV in tumorigenesis can be understanded through the research of EBV biological characteristics, EBV+ lymphoma and EBV- lymphoma. Based on traditional therapy, supplement of specific treatment for EBV, such as antiviral treatment, immunotherapy, can improve the curative rate of lymphoma...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Dian Zhou, Wei Chen, Kai-Lin Xu
Essential thrombocythemia(ET) is one of the Ph chromosome-negative myeloproliferative neoplasms. Some studies discovered that the mutation of JAK2 V617F existed in 50%-70% patients with ET. Recently, many significant advances in researches about pathogenesis of ET, such as mutations of JAK2V617F, MPL, CALR and other related mutation; the epigenetic abnomalities in incidence of ET; the changes of bone marrow microenvironment of ET and the regulation of related cytokines were obtained. In this article, the advances of above mentioned aspects of ET are summarized...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jun-Xiu Liu, Wei Chen, Kai-Lin Xu
Myelofibrosis(MF) is a type of myeloprolifirative neoplasms which is difficult to be treated. With the discovery of V617F mutation site in Janus kinase 2 (JAK2), JAK inhibitor provides a new treatment strategy for patients with myelofibrosis. Since 2011 the FDA in USA approved the first generation of JAK inhibitor Ruxolitinib for marketing, a growing number of JAK inhibitors have been entering into the clinical trials and showed a certain clinical efficacy. On the one hand, some JAK inhibitors for single application can effectively relieve the clinical symptoms of patients with myelofibrosis, slow down disease progression, and prolong the survival; on the other hand, JAK inhibitor can also be applied in combination with traditional or other new targeted drugs for MF patients, even during the allogenetic hematopoietic stem cell transplantation, thus providing more choices for targeted therapy on the patients with myelofibrosis...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Hui-Ping Shi, Zhao-Yue Wang
Hereditary platelet disorders are a heterogeneous group of disorders characterized by abnormal number or function of platelets, sometimes even involving other systems apart from blood abnormalities. The great clinical and genetic heterogeneity makes the diagnosis and treatment of hereditary platelet disorders as a huge challenge for clinicians. At present, only a small number of patients have received a clear molecular diagnosis of hereditary platelet diseases, and a lot of pathogenic genetic variations still remain unknown...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Hao-Lan Wang, Bao-Shan Liu
Immune thrombocytopenic purpura (ITP) is a chronic and recurrent autoimmune disease, which seriously affects the life quality of patients. At present, a series of new advances have been made in the pathogenesis of ITP, particularly, in the abnormal cellular immunity. However, in the medical studiess generally research of ITP cellular immunity was limited. Therefore, it is urgent to establish an ideal ITP model for the study of ITP pathogenesis, so as to contribute to promote the ITP new treatment progeamme...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Rui Zhang, Ya-Nan He, Jun Yin, Wen-Yu Wu, Bei-Bei Wang, Hui-Ming Zhang
OBJECTIVE: To explore the occurrence and various influencing factors of the splenic extramedullary hematopoiesis in rats. METHODS: A total of 411 rats of different sex and species were assigned to this study. These rats were fed different feed in the same environment, and killed after 104 weeks. The spleen of all animals was embedded in paraffin, sectioned, stained with hematoxylin and eosin(H& E), then examined by optical microscopy to observe splenic extramedullary hematopoiesis...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xiao-Xia Zhang, Ya-Peng Han
OBJECTIVE: To study and analyse the application of ELISA and nucleic acid test (NAT) to HBV detection of voluntary blood donors in Qingyang city and the characteristics and social attributes of HBV positive population. METHODS: The HBV of 70 659 blood donors in Qingyang city from January 2013 to December 2016 was detected by using ELISA and NAT, and the statistical analysis of results was performed by means of X2 detection and FIsher' s Exact test. RESULTS: The HBV positive rate detected by ELISA was 0...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Sha-Sha Zhao, Shu Fang, Cheng-Ying Zhu, Li-Li Wang, Chun-Ji Gao
OBJECTIVE: To investigate the effect of granulocyte-colony stimulating factor (G-CSF) in vitro stimulation on the distribution of lymphocyte subset in healthy human. METHODS: Peripheral blood mononuclear cells (PBMNCs) were collected from 8 healthy volunteers by density gradient centrifugation on Ficoll-PaqueTM. In vitro 200 ng/ml G-CSF or 200 ng/ml G-CSF plus 10 µg/ml ConA directly act on PBMNCs, then the colleted cells were cultivated for 3 days. Lymphocyte subsets were stained with the corresponding fluoresce labeled antibodies and detected by flow cytometry...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Wei-Jiang Liu, Xue Li, Yuan-Lin Liu, Peng Wang, Yue Fan, Tian-Yi Teng, Bo-Qian Bai, Yue Tong, Wei Zhang, Yi Zhang
OBJECTIVE: To investigate the effect of MicroRNA-3963(miR-3963) on the adipogenic differentiation of mouse bone-derived mesenchymal stem cells(MSC). METHODS: MSCs were isolated from C57BL/6 mice bone fragment and transfected with miR-3963 mimic, miR-3963 inhibitor and negative control. The expression of miR-3963 and transfection efficiency were detected by q-PCR. These transfected cells were induced to adipocytes and stained with oil red O after 14 days culture...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xiao-Li Xu, Zhuo-Wen Chen, Ping Zou
OBJECTIVE: To explore the effects of IFN-γ on pulmonary GVHD after hematopoietic stem cell transplantation (HSCT). METHODS: The mouse GVHD models were established by using C57BL/6J, B6D2F1 mice and different HSCT. According to HSCT modes, the mice were divided into 3 groups: syngeneic HSCT group(C57BL/6J→C57BL/6J), allogeneic HSCT group (C57BL/6J→B6D2F1) and IFN-γ -/- allogeneic HSCT group (IFN-γ -/-→C57BL/6J→B6D2F1). The survival time, GVHD clinical seore, pulmonary pathologic changes in 3 groups were compared and analyzed, and the total cell count in BALF and IFN-γ level in serum were detected in 3 groups after transplantation...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Qi Hao, Shu-Qin Zhang, Dong-Mei Zhang, Jing-Bo Wang, Jie Zhao, Hao-Yu Cheng
OBJECTIVE: To investigate the value of flow cytometry (FCM) detection in prognostic evaluation of minimal residual disease (MRD) of acute myeloid leukemia (AML) patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT). METHODS: Eighty-two cases of AML (except M3) after allo-HSCT who accord to enrolled condition (no MRD positive after allo-HSCT confirmed by regular flow cytometry detection and followed-up for 2 years) from April 2012 to September 2016 in our department were selected...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Guang Tang, Xiao-Min Wang, Jun-Xia Meng, Chun-Lai Luan, Jie-Fu Chen, Yong-Qiang Wu, Xiao-Nan Zhang, Zi-Yan He
OBJECTIVE: To evaluate and compare the clinical efficacy and safety of recombinant human thrombopoietin(rhTPO) and recombinant human interleukin11(rhIL-11) for the treatment of chemotherapy-induced thrombocytopenia in adult acute myeloid leukaemia patients. METHODS: Total of 96 adult acute myeloid leukaemia patients were divided into 3 groups according to randomized controlled method: rhTPO group, rhIL-11 group and control group, 32 cases in each group. The patients in rhTPO group and rhIL-11 received rhTPO of 15000 IU/d and rhIL-11 of 1...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Run-Mei Tian, Ying Rong, Liu-Song Wu, Yan Jiang, Hai-Yan Qing, Ping Zhu, Yan Chen
OBJECTIVE: To explore the mutation and single nucleotide polymorphism(SNP) of LNK gene in the patients with essential thrombocytosis (ET), and to analyze the relationship between LNK gene variation and the occurrence of ET. METHODS: JAK2V617F mutation was identified by allele-specific PCR. The whole exon of LNK gene was amplified by PCR. The amplified sequences included the Rs3184504 (C/T) and Rs78894077 (A/C/G/T) affecting the expression of amino acids in LNK gene, and the Rs7973120 (A/T) unaffecting the expression of amino acids...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Gang Wu, Yu-Han Duan
OBJECTIVE: To study the positive distribution rate of Coombs test in patients with clinical anemia and blood transfusion, and its effect on clinical blood transfusion. METHODS: Seventy patients with hemoglobin level in the normal range were enrolled into control group, while 130 patients with anemia or blood transfusion who' s hemoglobin level was lower comfirmed by micro-column gel antihuman globin detection card and 70 surgical patients with anemia or blood transfusion who' s hemoglobin level was lower comfirmed by micro-column gel anti-human globin card were enrolled into anemia or blood transfusion (A or BT) group...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Wen-Qian Cai, Xi-Jiang Hu, Qian Xiong, Bin Zhou
OBJECTIVE: To investigate the genotypes of newborn α-thalassemia and to analyze its characteristics of molecular epidemiology in Wuhan area. METHODS: The newborn α-thalassemia gene in 1376 cases with positive confirmed in the primary screening in Wuhan area was detected by PCR, flow cytometry and FISH. RESULTS: The α-thalassemia in 436 newborns and α- β-composite thalassemia in 10 newborns were confirmed by detection in Wuhan area. The majority of thalassemia cases [up to 92...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ying-Qi Shao, Hong-Yu Dong, Mei-Li Ge, Feng Zhang, Jun Shi, Xing-Xin Li, Jin-Bo Huang, Zhen-Dong Huang, Jing Zhang, Neng Nie, Yi-Zhou Zheng
OBJECTIVE: To detect the expression of miRNA in de novo and complete response SAA patients and predict the targets of the miRNAs. METHODS: The expression profiles of miRNA from bone marrow mononuclear cells of the SAA patients with de novo and CR were detected by miRNA microarray. RESULTS: Totally 35 up-regulated and 37 down-regulated miRNA were identified in CR SAA patients in comparison with de novo SAA patients. Furthermore, by predicting the targets of the differentlly expressed miRNA, it was found that some targets associated with T cell receptor signaling pathway and cell adhesion molecules...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xue-Fang Zhang, Shu-Ying Zhang, Hong Zhou, Jia-Wei Feng, Li-Bo Jiang, Yan-Zhang Qu, Heng Guo, Shu-Liang Wang
OBJECTIVE: To explore the relationship between expression of CD96 and CD123 and prognosis of patients with myelodysplastic syndrome(MDS). METHODS: Eight-nine MDS patients(MDS group) and 20 persons without hematologic disease as controls(Control group) were enrolled. The patients were grouped by the risk. All participants received bone marrow biopsy. Mononuclear cells were extracted, CD34+CD38-CD123+ and CD34+CD38-CD96+ cells were counted by using flow cytometry...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
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