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Zhongguo Shi Yan Xue Ye Xue za Zhi

Zhi-Jun Bao, Xu Liu
Multiple myeloma (MM), the second common malignant tumor in the blood system, is a kind of B cell malignancy and its prognosis is poor. The diagnosis of MM is mainly based on the number of abnormal plasma cells and the presence of monoclonal protein in the blood or urine, but there are limitations for diagnosis of MM. Multi-parameter flow cytometry analysis is increasingly used in the in the diagnosis of MM. Therefore, this review focuses on characteristics of immunophenotypes of malignant plasma cells and myeloma progenitor cells...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yu-Jie Wang, Bao-An Chen
Hematologic disease has become a common disease that produces harm and burdens to human individuals today, seriously threatening public health care. Particularly, the damage caused by malignant hematologic disease has been recognized by doctors and patients. However, low cure rate and high recurrence rate are the main issues when treating malignant hematologic disease by current methods. The survival rate could be significantly improved if the early metaphase diagnosis rate could be improved. Therefore, early diagnosis is important for malignant hematologic disease at molecular level...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yu-Lu Wu, Jian-Lin Qiao, Kai-Lin Xu, Ling-Yu Zeng
Platelets are known to play a critical role in thrombosis and hemostasis. However, recent studies demonstrated that beyond their role in thrombosis and hemostasis, platelets are also involved in the regulation of tissue repair and regeneration. Increasing number of studies on the roles of platelets in tissue repair showed that various growth factors, chemokines as well as cytokines secreted from activated platelets regulate injured tissue repair and regeneration with the main mechanisms being through regulation of cell migration, proliferation, and angiogenesis, cell apoptosis and survival...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ling-Ling Fu, Run-Hui Wu
Immune thrombocytopenia (ITP) is the most common immune-mediated acquired bleeding disorders in children. The prognosis of majority of these patient are well, with recent complete remission. However, a few patients are facing the risk of bleeding since they do not respond to the first-line therapy, and need second-line therapy. The present second-line treatments are difficult to be popularized due to their efficacy, side-effects, costs and some other factors. The pulsed high-dose dexamethasone (HDD) therapy with its good effect, small adverse effects and low cost in adults has been used as the first-line therapy in newly diagnosed ITP, which now has attracted the attention of pediatricians...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Shao-Hua Chen, Xiang-Feng Zha, Yang-Qiu Li
The main mechanism of tumor immune suppression is due to the T cell exhaustion which is mediated by abnormal expression of T-cell immunosuppressive receptors in immune cells. Blocking these molecules may restore partial or all functions of T cells. This article reviews the advance on the role of the newly discovered T cell immuno-suppressive receptors such as TIM-3, LAG-3 and BTLA, including their mediated T cell-immune tolerance and the study of targeted immunotherapy in hematological malignancies, so as to provide the new strategy for immune-targeted therapy for hematological malignancies...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Zuo-Fei Chi, Wei Yang, Qiu-Ying He, Shuang Fu, Yu Fu
Necroptosis is a novel programmed cell death mechanism which is characterized by a necrotic morphology, but the necroptosis is precisely regulated by cellular signaling pathway just like apoptosis. Recently, many reports have revealed that necroptosis contributes to the pathogenesis of inflammation, ischemic reperfusion injury and tumour. Hematological malignancies are a set of hemotopathy diseases with poor prognosis. In this review, the research progress of signaling pathway of necroptosis and its role in hematological malignancies are summarized...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Hai-Yun Li, Cong-Yuan Cheng
OBJECTIVE: To understand the regularity of Rh blood typing of valunteary blood donors in Chinese Nanyang city, and to estabbish a Rh DC/c/E/e antigen negative donor base so as to provide the help for clinical emergent blood transfusion to patients and ensure the safety of blood transfusion. METHODS: The Rh D blood group of blood samples from 81462 voluntary blood donors in Chinese Nanyang city in 2014 was identified by serologic method; after first screening and confirmation, the RhE/e/C/c typing of Rh D negative samples was performed; the detailed infornation of donors was registered seriously by using unified creteria; the data base and base in kind of RhE/e/C/c types of valuntary donors were established by means of compater-mamayement system...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Fang Ye, Li-Ping Zhang
OBJECTIVE: To explore the effect of red blood cell(RBC) transfusion on T lymphocyte subsets in the patients with hematologic malignancies. METHODS: The patients admitted in department of hematology of the second hospital of Shanxi Medical University from September 2013 to September 2014 acconuted for 144 cases had been diagnosed with hematologic malignancies and needed RBC transfusions according to clinical transfusion creteria (Hb level<70 g/L). All the patients were in rest period after chemotherapy, furthermore they did not administrate immunomodulatory drugs...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Shan-Hai Ou, Jin-Zhen Xie, Ya-Li Zhang, Hong-Ying Ni, Xiu-Yu Song
OBJECTIVE: To estimate the prevalence of parvovirus B19 infection in Chinese Xiamen area blood donors. METHODS: Blood samples from blood donors were tested for detection of parvovirus B19 DNA and antibody. The direct sequencing and genetype analysis of B19 DNA positive samples were performed. RESULTS: Six out of 10452 samples were B19 DNA positive. The viral loads of the 6 samples were between 3.59×10(2)-1.07×10(4) IU/ml; the positive rate of B19-IgM was 4...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yan-Hui Qiao, Hong-Bin Zhang, Wei-Peng Guo
OBJECTIVE: To investigate the distribution of Colton and Diego rare blood group antigens of blood donors in Chinese Xinjiang minorities. METHODS: A multiplex PCR was applied to screen for alleles antigens Di(a) and Co(b) in 1020 randomly selected healthy donors of Chinese Xinjiang minorities by using each 5 samples mixed detection method. The samples in the positive pools were further tested individually. Furthermore, the positive samples, including Di((a+b-))/Di((a+b+)) and Co((a-b+))/ Co((a+b+)) genotypes were tested via 2 PCR-SSP assays for high frequency allele Di(b) and Co(a) to get the rare genotypes Di((b-)), Co((a-))...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Gui-Ji Li, Xu Zhang
OBJECTIVE: To investigate and identify the molecular and biological characteristics of B305 blood group gene subtypes of a ABO variant. METHODS: The individual was confirmed by standard serological techniques. The genotyping and sequencing were performed by using polymerase chain reaction-sequence specific primer (PCR-SSP), direct sequencing and gene cloning for Exon 6 and exon 7 of ABO locus respectively. RESULTS: B antigen was detected on red blood cells of the proband and there was anti-A antibody in the serum...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yong-Feng Pei, Hui-Ni Huang, Heng-Cong Li, Guo-Guang Wu
OBJECTIVE: To identify a novel human leukocyte antigen (HLA) allele HLA-B*13:92 and analyze 3D model of HLA molecule. METHODS: Polymerase chain reaction sequencing-based (PCR-SBT) was used in routine HLA typing, the B locus typing results of one sample was one base mismatch with B*13:01:01, B*58:01:01 at locus 189, The Group Specific Sequencing Products (GSSP) which target at B*13 and B*58 were used to confirm difference between the new allele and highest homologous allele, then the new allele was modeled by Swiss-model to its 3D structure...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ang Li, Xing Sun, Ke-Ke Fan
OBJECTIVE: To verify the role of the newly identified mitotic regulator candidate pre-mRNA processing factor 19 (Prp19) in mitosis and to clarify its underlying mechanism. METHODS: FACS analyses with propidium iodide (PI) staining were performed to evaluate the effect of Prp19 knockdown on cell cycle distribution. To further clarify the role of Prp19 in mitosis, the effect of Prp19 depletion was monitored by time-lapse imaging of HeLa/GFP-H2B cells. Cold treatment experiment was used to examine the effect of Prp19 knockdown on the attachment of microtubules and kinetochores...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Hai-Ning Li, Shao-Lin Zhao, Jin Yang, Wei Li
OBJECTIVE: To explore the change trend of cell viability, apoptosis and reactive oxygen species (ROS) production of peripheral blood neutrophils in in vitro culture. METHODS: Neutrophils were isolated by density gradient centrifugation and cultured in medium containing 10% fetal bovine serum for diffentrent times in a fall humidified atmosphere with 5% CO2 at 37 °C. Cell viability, spontaneous apoptosis and ROS level were detected by flow cytometry at 0, 24, 48, 72 and 96 hours...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Wei Zhang, Jing Wang, Yan-Fang Wang, Ming-Xia Zhu, Wen-Li Wan, Hai-Shen Li, Fei-Fei Wu, Xin-Xing Yan, Xiao-Yan Ke
OBJECTIVE: To investigate the expression and subcellular distribution of costimulatory molecules B7-H1, B7-H3 and B7-H4 in human hematologic malignancy cell lines. METHODS: The expression and subcellular distribution of B7-H1, B7-H3 and B7-H4 in 13 human hematologic malignancy cell lines were determined by RT-PCR, qPCR, Western blot and flow cytometry, the peripheral blood mononuclear cells (PB MNC) of 12 volunteers were used as control. RESULTS: The mRNA of B7-H1, B7-H3 and B7-H4 was widely expressed in PB MNC and hematologic malignancy cell lines, with a lower level of B7-H4...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Wei-Jun Gu, Qing-Guo Zhang, Wei Zhu, Yu-Lin Guo, Lu-Qin Zhang
OBJECTIVE: To investigate the imbalance of Tc1/Tc2,Th1/Th2 and Tc/Th in patients with systemic lupus erythematosus(SLE) and its relationship with the clinical stages of SLE. METHODS: The full blood culture and flow cytometry with fluorescence-labelled T lymphocytes were used to detect the levels of T-lymphocyte subsets and its intracellular factors IFN-γ and IL-4 in peripheral blood from SLP patients in active, inactive stages and normal healthy persons as controls, to compare the changes of Tc1,Tc2; Th1,Th2 levels and Tc/Th ratio in SLP patients in active and inactive stages, and to analyze their relationship with SLEDAI staging...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xiao-Ning Wang, Jian-Na Yao, Fang Liu, Xiao-Juan Wang, Juan-Juan Ma, Cai-Li Guo, Hua-Sheng Liu, Peng-Cheng He, Mei Zhang, Chun-Hong Sun
OBJECTIVE: To observe the efficacy and adverse reactions of autologous PBSC collection when the autoPBSC procedure and MNC procedure of COBE Spectra cell separator and the MNC procedure of Spectra Optia cell separator were used. METHODS: The autologous perepheral blood hematopoietic stem cells from 41 patients were collected by using autoPBSC procedure and MNC procedure of COBE Spectra blood cell separator and MNC procedure of Spectra Optia blood cell separator...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Qi Wang, Si-Qiao Sun, Zhi-Hua Cheng, Zhong-Ying Wang, Chao Liu
OBJECTIVE: To explore the relationship among the serum D- two polymer, fibrinogen, C reactive protein and interleukin 6 and thrombus dissolution volume in acute iliac femoral venous thrombosis model rats. METHODS: A total of 60 rats were randomly divided into 3 groups: deep venous thrombosis group (DVT group), sham operation group and normal control group. In DVT group the single side of the iliofemoral vein incomplete with micro vessel was cliped under chloral hydrate anesthesia; in sham operation group the single side of the iliofemoral vein should be explored without using micro vessel clip under chloral hydrate anesthesia; the and normal control group only experienced chloral hydrate anesthesia...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Li-Juan Tang, Xiao-Heng Zhou, Qiong Bin, Xiao-Hua Huang, Jiang-Ming Luo
OBJECTIVE: To investigate the frequency distribution of human platelet antigen allele HPA-2 and HPA-15 among the pediatric patients with acute or chronic idiopathic thrombocytopenic purpura (ITP) in Chinese Guangxi area, and to explore the potential correlation of ITP with HPA-2 and HPA-15 gene polymorphisms. METHODS: The clinical and laboratorial data of 46 children diagnosed as acute ITP and 46 children diagnosed as chronic ITP between January 2007 and December 2014 were collected...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Shan-Shan Ma, Seong-Hyun Ho, Hong-Ling Wang, Li-Ya Nie, Song-Shan Xu
OBJECTIVE: To evaluate the effect of PEGylated IL-11 mutein (PEG-mIL 11) with different dose or injection frequency on thrombocytopenia in myelosuppressed mice and to compare its effect with mIL-11, so as to provide reference data for clinical use. METHODS: Myelosuppressive model with thrombocyopenia was produced in BALB/c mice by whole body (60)Co γ-ray irradiation in dose of administration 2.5 Gy followed by i.p. injections of carboplatin at 50 mg/kg. In study of injection frequency, 30 thrombocytopenic BALB/c mice were randomly divided into 5 groups: vehicle control group (once daily on d1, 4, 7), mIL-11 group [200 µg/(kg·d)×9 d], PEG-mIL 11 A group [111800 µg/(kg·d)×1 d (d 1)], PEG-mIL 11 B group 900 µg/(kg·d)×2 d (d 1,5), and PEG-mIL 11 C group [600 µg/(kg·d)×3 d (d 1,4,7)]...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
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