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Zhongguo Shi Yan Xue Ye Xue za Zhi

Jing-Jing Xu, Hui-Xia Xiong
Ph-like acute lymphoblastic leukemia (ALL) is a high-risk subtype of precursor B-cell acute lymphoblastic leukemia (BCP-ALL) with a gene expression profile and a high frequency of IKZF1 gene alteration similar to that of Ph-positive ALL, which is a clinically and biologically heterogeneous subtype of BCP-ALL. The prognosis correlats negatively with age increasing. The incidence of this "Ph-like" subtype may be higher in young adults. Ph-like ALL is characterized by genetic alterations that activate cytokine receptor genes and kinase signaling pathways...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yan-Bin Pang, Li-Xia Fan, Hua Xue, Jia Liu, Xin DU, Luo-Ming Hua, Jing Wang
Hypomethylating agents(HMA) currently are widely used in the treatment of myelodysplastic syndromes (MDS), provide a significant improvement in the treatment of MDS. However, resistance to HMA is an almost universal phenomenon. This review was focused on immune effects related to DNA methylation, and to explore the mechanism underlying HMA resistance involved in immune checkpoint pathways. However, the optimal role of checkpoint blockade therapy (CBT) and immune checkpoint pathways remain in HMA failure questionable...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xue Yang, Chun-Ze Tan, Ju-Lin Li
Glucose-6-phosphate dehydrogenase deficiency (G6PD) is one of hereditary diseases sariously influencing the human health. G6PD is characterized by wide distribution, high incidence, inducing the hemolysis, complex mechanism of hemolysis and common occurence in children and so on. The blood transfusion is most effective method for acute ouset of hemolysis, but the risk is more high, thereby it is necessary to guarante the safety of blood transfusion. In addition of the routine managementin blood transfusion and standard procedures, the basic rules and indicators of blood transfusion must be grasped, the blood preparations should be known well, the blood protection must be strengthened, and the measures of personalized psychologic interference must be drawn up carnestly and performed strictily so as to improve the therapeutic efficacy and safe of blood transfusion...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Nan Yang, Chun-Ji Gao
BK virus(BKV)associated hemorrhagic cystitis is one of the most common complications after hematopoietic stem cell transplantation(HSCT), which can increase the suffering, become time consuming and even life threatening. However, BKV infection has not attracted enough attention in clinical practice. Accurate BKV monitoring and effective clinical treatments are still in urgent need. In recent years, BKV-DNA monitoring has shown a significant clinical value in BKV-associated hemorrhagic cystitis after HSCT...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Zhen-Hua Wang, Hui-Hui Liu, Ning Ma, Li-Hong Wang, Wei Liu, Bo Tang, Zhi-Xiang Qiu, Xi-Nan Cen, Han-Yun Ren, Yu-Jun Dong
OBJECTIVE: To investigate the expression of CD160 on the surface of human natural killer (NK) cells and its possible relationship with hematological malignancies. METHODS: CD160 expression on human leukemia cell line NK92 cells was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The proliferation characteristics and cell surface markers of this cell line were determined. Cytotoxicity of NK92 against 2 human myeloid leukemia cell lines, K562 and THP-1 was analyzed ex vivo...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Shu-Jun Wang, Xiao-Bao Shao, Na Li, Guang-Chao Zhao, Tao Hou, Wen Tang, Jun Yuan, Cai Chen, Meng-Qi Xue, Lu Wang, Jian-Feng Luan, Pei-Yuan Zhu
OBJECTIVE: To study the coagulation properties the refrigerated whole blood stored at 4℃. METHODS: Ten units of whole blood were obtained from healthy volunteer donors and stored at 4±2℃ for 21 days. Samples were collected on the day after donation and on days 2, 4, 6, 8, 10, 14 and 21 for delection including complete blood count, electrolyte, APTT, PT, Fg, blood coagulation factors, and thromboelastography(TEG). RESULTS: The levels of Hb, WBC, Plt, sodium and potassium in each sample accorded with standard of storing whole blood...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Tong-Bo Liu, Mao-Quan Wang, Wei Guan, Yu Jing
OBJECTIVE: To evaluate the diagnostic values of procalcitonin(PCT), C-reactive protein(CRP) and interleukin-6 (IL-6) in patients suffered from hematological diseases with bacterial infection, and to provide further evidence for clinical application. METHODS: A total of 3631 blood cultures, serum levels of PCT and CRP and IL-6 (n=1587) from the inpatients from 2014-01-02 to 2018-01-27 were retrospectively analyzed. The patients were divided into positive (n=208) and negative blood culture (n=3423) groups...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Wei Chen, Dian Zhou, Ya-Ru Li, Jun-Xiu Liu, Xiang-Min Wang, Feng Zhu, Kai-Lin Xu
OBJECTIVE: To explore the effects of lentiviral-mediated CXC chemokine receptor-4(CXCR-4)gene over-expression on the homing capacity of mesenchymal stem cells(MSC)in vivo. METHODS: The MSC overexpressing CXCR-4 were constructed by using the lentiviral vector-mediated mouse MSC overexpressing the CXCR-4 gene. The BALB/c mice were divided into 3 group: simple radiation group(TBI)in which mice exposed to total body irradiation, then were infused with normal saline; EGFP-MSC group in which mice were infused with MSC(5×105 )transducted by EGFP via tail vein after TBI; and CXCR-4-MSC group in which mice were infused with MSC (5×105 ) simultaneously carraying EGFP and CXCR-4 gene via tail vein after TBI...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Su-Yan Bian, Hong-Bin Liu, Hong-Wei Liu, Qi-Wei Zhu
OBJECTIVE: To explore the proangiogenic activity of exsomes released by human umbilical cord mesenchymal stem cells (MSCs) stimulated by erythropoietin and platelet-derived growth factor BB (PDGF-BB). METHODS: Human umbilical cord-derived MSCs were seeded and maintained in culture overnight. The media were then replaced by alpha-MEM containing EPO (1 U/ml) and/or PDGF-BB (50 ng/ml), and the culture was maintained for 72 hours. The exosomes from the culture supernatants were isolated with a routine ultra-catrifagation method...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ling Fang, Xue-Feng Shi, Hui-Yan Sun, Yu-Xiang Li, Feng-Jun Xiao, Hua Wang, Li-Sheng Wang
OBJECTIVE: To investigate the effects of exosomes derived from miR-486 gene-modified umbilical cord mesenchymal stem cells (UC-MSCs) on biological characteristics of rat cardiomyocytes. METHODS: The human umbilical cord mesenchymal stem cells (UC-MSCs) were isolated and cultured, then the immunophenotypes and ability of osteogenic and adipogenic differentiation of UC-MSC were identified. The structure of exosomes was observed by electron microscopy; the effect of exosomes on cell migration was detected by transwell cell migration test; the miR-486 high expression of UC-MSC was mediated by using recombinant adenovirus vector, moreover the UC-MSC with high expression of miR-486 were identified by qPT-PCR...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jing Guo, Shu-Yue Wang, Xiao-Feng Zhu, Shu-Tan Li, Fan-Li Lin, Xiao-Ming Li, Chun-Lan Huang
OBJECTIVE: To study the effect and mechanism of shh and mesenchymal stem cell(MSC)synergism on the proliferation of hematopoietic stem cells in noninvasive co-culture system in vitro. METHODS: The mesenchymal stem cells were cultured in vitro,CD34+ cells were sorted by mini MACS magnetic bead separator,flow cytometry was used to identify the purity of 2 cells. CD34+ cells and MSCs were seeded to upper and low of transwell respecibely for non-contact coculture,and add exogenous shh protein for intervenece...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jing-Bo Wang, Lei Yuan, Hao-Yu Cheng, Xin-Hong Fei, Jiang-Ying Gu, Song Xue, Jun-Bao He, Wei-Jie Zhang
OBJECTIVE: To investigate the efficiency and safety of treating Epstein-Barr virus (EBV) infection of acute graft versus host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) by EBV specific cytotoxic T lymphocytes (EBV-CTL). METHODS: The Clinical characteristics, therapeutic efficacy and safety of 12 patients with EBV infection treated by EBV-CTL infusion after allo-HSCT in Department of Hemahlogy of Aero Space Center Hospital between Jan 2015 and May 2017 were analyzed retrospectioely...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Peng Hu, Tong-Hua Yang, Ren-Bin Zhao, Ke-Qian Shi, Yan-Mei Yang, Xun Lai
OBJECTIVE: To investigate the efficacy of hematopoietic stem cells cryopreserved by ladder-style freezing from low temperature refrigerator to liquid nitrogen in treatment of hematological malignancies, and to analyze the survival condition of patients after hematopoietic stem cell transplantation. METHODS: The coyoprotectant formed by 3% hydroxyethyl starch, 4% albumin and 5% dimethyl sulfoxide (DMSO) was need for cryopreservation of hematopoietic stem cells,which were first placed in -800 C low temperature refrigerator and then were stored in -1960 C liquid nitrogen tank...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xiao-Fang Hou, Hai-Long Xia
OBJECTIVE: To explore the immunopathogenesis of Henoch-Schonlein purpara (HSP) by detecting the levels of Th17 cells, IL-17 and matrix metallo proteinase-13 (MMP-13) in peripheral blood and the expression of IL-17 in skin lesions at acate phase of HSP. METHODS: Th17 cell ratio in peripheral blood of HSP group and healthy control group was defected by flow cytometry, the plasma levels of IL-17 and MMP-13 in HSP group and healthy control group were defected by ELISA, and expression level of IL-17 in skin lesion of HSP group and skin tissue of healthy control group was deternined by: immunohistochemistry method...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Shi-Xiang Zhao, Zhi-Xiang Lu, Li-Qun Yu, Xin Guan, Hai-Ping He, Ke-Qian Shi, Ren-Bin Zhao, Tong-Hua Yang
OBJECTIVE: To better define the effect of JAK2V617F mutant allele burden on clinical presentation of patients with essential thrombo cythamia (ET), especially thrombosis. METHODS: Two ml of heparin anti-coagulated bone marrow was collected from 229 ET cases, who were diagnosed and treated in the First People's Hospital of Yunnan Province during 2013.10 to 2016.12. and then the mononuclear cells were separated by Red Blood Cell Lysis Buffer, genomic DNA was extracted from mononuclear cells by using a commercial DNA isolation kit and amplified by allele specific polymerase chain reaction (PCR)...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Qun-Fang Jiang, Qian Xiao, Fu-Sheng Jiang, Feng Liu
OBJECTIVE: To investigate the effects of storage lesion in apheresis platelets on platelet apoptosis and the changes of aggregation function, and analyze the relationships between the apoptosis and aggregation function. METHODS: Platelet samples were collected from 10 healthy donors with O blood group. Firstly, the effects of storage lesion in platelets on the platelet apoptosis were detected by flow cytometry. Then, using a multiplate analyzer, individual-donor Plt aggregation response to stimulation by the agonists ADP, Collagen, TRAP and ASPI was examined...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Tao Zhong, Wei Xu, Hai-Liang Hu, Shu-Jun Liu, Hui-Qin Wen, Kang Xia, Mao-Hong Bian
OBJECTIVE: To evaluate the performance of thromboelastography (TEG) to monitor in vivo blood coagulation status and the efficacy of antiplatelet aggregation drugs in the patients with coronary heart disease (CHD) after oral anticoagulation. METHODS: Seventy one CHD patients were enrolled in CHD group and 380 healthy persons with normal TEG were enrolled in the control group. After admission, all CHD patients were administrated with routine anti-platelet aggregation drugs at a clinically recommended dose...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Feng Yu, Xiao-Yang Yang, Meng-Jie Wan, Mei-Qing Lei, Ling-Feng Sun, Xu Han, Wen-Jin Li, Fang-Ping Chen
OBJECTIVE: To investigate the effects of synthetic long-chain polyphosphate on blood coagulation and platelet aggregation. METHODS: The effect of artificial synthetic long chain poly phosphate on blood coagulation and platelet aggregation was detected by coagulation tests, coagulation factor activity detection and platelet aggregation test, and its mechanism was explored by ELISA, flow cytometry and high content imaging system. RESULTS: The long chain polyphosphates prolonged activated partial thromboplastin time, decreased coagulation factor FⅧ, FⅨ, FⅪ and FⅫ activity, blocked ADP-induced platelet aggregation, and decreased the concentration of calcium and TXA2 in platelet...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Hui Liu, Na Sun, Yu-Lan Huang, Xie Su, Dong-Hong Deng, Peng Cheng
OBJECTIVE: To investigate the changes of E-selectin, thrombin-antithrombin complex(TAT), prothrombin fragment 1+2(F1+2), tissue factor(TF)and tissue factor pathway inhibitor(TFPI)before and one year after splenectomy in thalassemia patients. METHODS: A total of 30 thalassemia patients undergoing electric laparoscopic splenectomy and 30 normal controls(NC) were enrolled in the study.Plasma levels of E-selectin, TAT, F1+2, TF and TFPI were detected by enzyme-linked immuno sorbent assay(ELISA)...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Li Wang, Lian-Chun Huang, Yao-Yun Li, Qi-Rong Feng, Gui-Ping Liao, Jun-Hong Chen, Ya-Li Zhou, Tian-Hong Zhou, Xiao-Lin Yin
OBJECTIVE: To investigate the oxidative stress status and its effects on hepcidin in patients with hemoglobin H Constant Spring disease (HbH-CS). METHODS: A total of 35 patients were enrolled in the study, including 15 splenectomized cases and 20 non-splenectomized cases. 20 healthy volunteers were selected as controls. Serum superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), oxidized glutathione (GSSG) levels, erythropoietin (EPO), serum free transferrin receptor (sFTR), growth differentiation factor 15 (GDF15) as well as the level of hepcidin were detected...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
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