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BMC Biochemistry

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https://www.readbyqxmd.com/read/28320308/binding-of-smoothelin-like-1-to-tropomyosin-and-calmodulin-is-mutually-exclusive-and-regulated-by-phosphorylation
#1
Annegret Ulke-Lemée, David Hao Sun, Hiroaki Ishida, Hans J Vogel, Justin A MacDonald
BACKGROUND: The smoothelin-like 1 protein (SMTNL1) can associate with tropomyosin (Tpm) and calmodulin (CaM), two proteins essential to the smooth muscle contractile process. SMTNL1 is phosphorylated at Ser301 by protein kinase A during calcium desensitization in smooth muscle, yet the effect of SMTNL1 phosphorylation on Tpm- and CaM-binding has yet to be investigated. RESULTS: Using pull down studies with Tpm-Sepharose and CaM-Sepharose, we examined the interplay between Tpm binding, CaM binding, phosphorylation of SMTNL1 and calcium concentration...
March 21, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28302049/increased-enzymatic-hydrolysis-of-sugarcane-bagasse-by-a-novel-glucose-and-xylose-stimulated-%C3%AE-glucosidase-from-anoxybacillus-flavithermus-subsp-yunnanensis-e13-t
#2
Yang Liu, Rui Li, Jing Wang, Xiaohan Zhang, Rong Jia, Yi Gao, Hui Peng
BACKGROUND: β-Glucosidase is claimed as a key enzyme in cellulose hydrolysis. The cellulosic fibers are usually entrapped with hemicelluloses containing xylose. So there is ongoing interest in searching for glucose- and xylose-stimulated β-glucosidases to increase the efficiency of hydrolysis of cellulosic biomass. RESULTS: A thermostable β-glucosidase gene (Bglp) was cloned from Anoxybacillus flavithermus subsp. yunnanensis E13(T) and characterized. Optimal enzyme activity was observed at 60 °C and pH 7...
March 16, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28264651/interactions-of-histatin-3-and-histatin-5-with-actin
#3
Edna Blotnick, Asaf Sol, Gilad Bachrach, Andras Muhlrad
BACKGROUND: Histatins are histidine rich polypeptides produced in the parotid and submandibular gland and secreted into the saliva. Histatin-3 and -5 are the most important polycationic histatins. They possess antimicrobial activity against fungi such as Candida albicans. Histatin-5 has a higher antifungal activity than histatin-3 while histatin-3 is mostly involved in wound healing in the oral cavity. We found that these histatins, like other polycationic peptides and proteins, such as LL-37, lysozyme and histones, interact with extracellular actin...
March 6, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28264645/systematic-substitutions-at-blip-position-50-result-in-changes-in-binding-specificity-for-class-a-%C3%AE-lactamases
#4
Carolyn J Adamski, Timothy Palzkill
BACKGROUND: The production of β-lactamases by bacteria is the most common mechanism of resistance to the widely prescribed β-lactam antibiotics. β-lactamase inhibitory protein (BLIP) competitively inhibits class A β-lactamases via two binding loops that occlude the active site. It has been shown that BLIP Tyr50 is a specificity determinant in that substitutions at this position result in large differential changes in the relative affinity of BLIP for class A β-lactamases. RESULTS: In this study, the effect of systematic substitutions at BLIP position 50 on binding to class A β-lactamases was examined to further explore the role of BLIP Tyr50 in modulating specificity...
March 6, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28118841/copper-chelation-and-interleukin-6-proinflammatory-cytokine-effects-on-expression-of-different-proteins-involved-in-iron-metabolism-in-hepg2-cell-line
#5
Luca Marco Di Bella, Roberto Alampi, Flavia Biundo, Giovanni Toscano, Maria Rosa Felice
BACKGROUND: In vertebrates, there is an intimate relationship between copper and iron homeostasis. Copper deficiency, which leads to a defect in ceruloplasmin enzymatic activity, has a strong effect on iron homeostasis resulting in cellular iron retention. Much is known about the mechanisms underlying cellular iron retention under "normal" conditions, however, less is known about the effect of copper deficiency during inflammation. RESULTS: We show that copper deficiency and the inflammatory cytokine interleukin-6 have different effects on the expression of proteins involved in iron and copper metabolism such as the soluble and glycosylphosphtidylinositol anchored forms of ceruloplasmin, hepcidin, ferroportin1, transferrin receptor1, divalent metal transporter1 and H-ferritin subunit...
January 24, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/27905872/knockdown-of-ggct-inhibits-cell-proliferation-and-induces-late-apoptosis-in-human-gastric-cancer
#6
Wenjie Zhang, Lei Chen, Honggang Xiang, Chunhua Hu, Weibin Shi, Ping Dong, Wenjie Lv
BACKGROUND: Gamma glutamylcyclotransferase (GGCT) has been proved to be involved in various cancers, but the biological function of GGCT in gastric cancer is still largely unknown. METHODS: The expression level of GGCT was evaluated by informatics analyses based on the Oncomine database. GGCT gene was then effectively knocked down via lentivirus mediated short hairpin RNA (shRNA) system. Then a series of functional assays, including MTT, colony formation and flow cytometry analysis were conducted on gastric cancer cells following GGCT knockdown...
December 1, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27716023/identification-of-replication-dependent-and-replication-independent-linker-histone-complexes-tpr-specifically-promotes-replication-dependent-linker-histone-stability
#7
Pei Zhang, Owen E Branson, Michael A Freitas, Mark R Parthun
BACKGROUND: There are 11 variants of linker histone H1 in mammalian cells. Beyond their shared abilities to stabilize and condense chromatin, the H1 variants have been found to have non-redundant functions, the mechanisms of which are not fully understood. Like core histones, there are both replication-dependent and replication-independent linker histone variants. The histone chaperones and other factors that regulate linker histone dynamics in the cell are largely unknown. In particular, it is not known whether replication-dependent and replication-independent linker histones interact with distinct or common sets of proteins...
October 1, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27974044/effects-of-protonation-on-the-hydrolysis-of-triphosphate-in-vacuum-and-the-implications-for-catalysis-by-nucleotide-hydrolyzing-enzymes
#8
Farooq Ahmad Kiani, Stefan Fischer
BACKGROUND: Nucleoside triphosphate (NTP) hydrolysis is a key reaction in biology. It involves breaking two very stable bonds (one P-O bond and one O-H bond of water), in either a concurrent or a sequential way. Here, we systematically examine how protonation of the triphosphate affects the mechanism of hydrolysis. RESULTS: The hydrolysis reaction of methyl triphosphate in vacuum is computed with protons in various numbers and position on the three phosphate groups...
June 29, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27009139/altered-activity-patterns-of-transcription-factors-induced-by-endoplasmic-reticulum-stress
#9
Sheena Jiang, Eric Zhang, Rachel Zhang, Xianqiang Li
BACKGROUND: The endoplasmic-reticulum (ER) responds to the burden of unfolded proteins in its lumen by activating intracellular signal transduction pathways, also known as the unfolded protein response (UPR). Many signal transduction events and transcription factors have been demonstrated to be associated with ER stress. The process in which ER stress affects or interacts with other pathways is still a progressing topic that is not completely understood. Identifying new transcription factors associated with ER stress pathways provides a platform to comprehensively characterize mechanism and functionality of ER...
March 24, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27001594/conserved-motif-viii-of-murine-dna-methyltransferase-dnmt3a-is-essential-for-methylation-activity
#10
Olga V Lukashevich, Natalia A Cherepanova, Renata Z Jurkovska, Albert Jeltsch, Elizaveta S Gromova
BACKGROUND: Dnmt3a is a DNA methyltransferase that establishes de novo DNA methylation in mammals. The structure of the Dnmt3a C-terminal domain is similar to the bacterial M. HhaI enzyme, a well-studied prokaryotic DNA methyltransferase. No X-ray structure is available for the complex of Dnmt3a with DNA and the mechanistic details of DNA recognition and catalysis by mammalian Dnmts are not completely understood. RESULTS: Mutant variants of the catalytic domain of the murine Dnmt3a carrying substitutions of highly conserved N167, R200, and R202 have been generated by site directed mutagenesis and purified...
March 22, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/26979141/linalool-isomerase-a-membrane-anchored-enzyme-in-the-anaerobic-monoterpene-degradation-in-thauera-linaloolentis-47lol
#11
Robert Marmulla, Barbara Šafarić, Stephanie Markert, Thomas Schweder, Jens Harder
BACKGROUND: Thauera linaloolentis 47Lol uses the tertiary monoterpene alcohol (R,S)-linalool as sole carbon and energy source under denitrifying conditions. The conversion of linalool to geraniol had been observed in carbon-excess cultures, suggesting the presence of a 3,1-hydroxyl-Δ(1)-Δ(2)-mutase (linalool isomerase) as responsible enzyme. To date, only a single enzyme catalyzing such a reaction is described: the linalool dehydratase/isomerase (Ldi) from Castellaniella defragrans 65Phen acting only on (S)-linalool...
March 15, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/26968444/1-2-dichlorobenzene-affects-the-formation-of-the-phosphoenzyme-stage-during-the-catalytic-cycle-of-the-ca-2-atpase-from-sarcoplasmic-reticulum
#12
Javier Vargas-Medrano, Jorge A Sierra-Fonseca, Luis F Plenge-Tellechea
BACKGROUND: 1,2-Dichlorobenzene (1,2-DCB) is a benzene-derived molecule with two Cl atoms that is commonly utilized in the synthesis of pesticides. 1,2-DCB can be absorbed by living creatures and its effects on naturally-occurring enzymatic systems, including the effects on Ca(2+)-ATPases, have been poorly studied. Therefore, we aimed to study the effect of 1,2-DCB on the Ca(2+)-ATPase from sarcoplasmic reticulum (SERCA), a critical regulator of intracellular Ca(2+) concentration. RESULTS: Concentrations of 0...
March 11, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/26956223/substrate-specificity-and-function-of-acetylpolyamine-amidohydrolases-from-pseudomonas-aeruginosa
#13
Andreas Krämer, Jan Herzer, Joerg Overhage, Franz-Josef Meyer-Almes
BACKGROUND: Pseudomonas aeruginosa, a Gram-negative, aerobic coccobacillus bacterium is an opportunistic human pathogen and worldwide the fourth most common cause of hospital-acquired infections which are often high mortality such as ventilator-associated pneumoniae. The polyamine metabolism of P. aeruginosa and particularly the deacetylation of acetylpolyamines has been little studied up to now. Results with other bacterial pathogens e.g., Y. pestis suggest that polyamines may be involved in the formation of biofilms or confer resistance against certain antibiotics...
March 9, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/26782782/characterization-of-a-cold-active-and-salt-tolerant-esterase-identified-by-functional-screening-of-arctic-metagenomic-libraries
#14
Concetta De Santi, Bjørn Altermark, Marcin Miroslaw Pierechod, Luca Ambrosino, Donatella de Pascale, Nils-Peder Willassen
BACKGROUND: The use of metagenomics in enzyme discovery constitutes a powerful approach to access to genomes of unculturable community of microorganisms and isolate novel valuable biocatalysts for use in a wide range of biotechnological and pharmaceutical fields. RESULTS: Here we present a novel esterase gene (lip3) identified by functional screening of three fosmid metagenomic libraries, constructed from three marine sediment samples. The sequenced positive fosmid revealed an enzyme of 281 amino acids with similarity to class 3 lipases...
January 19, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27474398/avoiding-false-discovery-in-biomarker-research
#15
Pranali Patel, Uros Kuzmanov, Seema Mital
BACKGROUND: Human tyrosine-protein phosphatase non-receptor type substrate 1α (SIRPA) is a surface marker identified in cardiomyocytes differentiated from human embryonic stem cells. Our objective was to determine if circulating SIRPA levels can serve as a biomarker of cardiac injury in children undergoing open heart surgery. RESULTS: Paired pre- and post-operative serum samples from 48 pediatric patients undergoing open heart surgery and from 6 pediatric patients undergoing non-cardiac surgery (controls) were tested for SIRPA protein levels using commercially available SIRPA ELISA kits from two manufacturers...
2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27455956/computational-studies-of-human-class-v-alcohol-dehydrogenase-the-odd-sibling
#16
Linus J Östberg, Bengt Persson, Jan-Olov Höög
BACKGROUND: All known attempts to isolate and characterize mammalian class V alcohol dehydrogenase (class V ADH), a member of the large ADH protein family, at the protein level have failed. This indicates that the class V ADH protein is not stable in a non-cellular environment, which is in contrast to all other human ADH enzymes. In this report we present evidence, supported with results from computational analyses performed in combination with earlier in vitro studies, why this ADH behaves in an atypical way...
2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27393145/effect-of-mutations-to-amino-acid-a301-and-f361-in-thermostability-and-catalytic-activity-of-the-%C3%AE-galactosidase-from-bacillus-subtilis-vtcc-dvn-12-01
#17
Thao Thi Nguyen, Hanh Van Vu, Nhung Thi Hong Nguyen, Tuyen Thi Do, Thanh Sy Le Nguyen
BACKGROUND: Beta-galactosidase (EC 3.2.1.23), a commercially important enzyme, catalyses the hydrolysis of β-1,3- and β-1,4-galactosyl bonds of polymer or oligosaccharidesas well as transglycosylation of β-galactopyranosides. Due to catalytic properties; β-galactosidase might be useful in the milk industry to hydrolyze lactose and produce prebiotic GOS. The purpose of this study is to characterize β-galactosidase mutants from B. subtilis. RESULTS: Using error prone rolling circle amplification (epRCA) to characterize some random mutants of the β-galactosidase (LacA) from B...
2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27387461/auto-thiophosphorylation-activity-of-src-tyrosine-kinase
#18
M Zulema Cabail, Emily I Chen, Antonius Koller, W Todd Miller
BACKGROUND: Intermolecular autophosphorylation at Tyr416 is a conserved mechanism of activation among the members of the Src family of nonreceptor tyrosine kinases. Like several other tyrosine kinases, Src can catalyze the thiophosphorylation of peptide and protein substrates using ATPγS as a thiophosphodonor, although the efficiency of the reaction is low. RESULTS: Here, we have characterized the ability of Src to auto-thiophosphorylate. Auto-thiophosphorylation of Src at Tyr416 in the activation loop proceeds efficiently in the presence of Ni(2+), resulting in kinase activation...
2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27387347/sustained-activation-of-mtorc1-in-macrophages-increases-ampk%C3%AE-dependent-autophagy-to-maintain-cellular-homeostasis
#19
Hongjie Pan, Xiao-Ping Zhong, Sunhee Lee
BACKGROUND: The mechanistic target of rapamycin complex 1 (mTORC1) is a well-conserved serine/threonine protein kinase that controls autophagy as well as many other processes such as protein synthesis, cell growth, and metabolism. The activity of mTORC1 is stringently and negatively controlled by the tuberous sclerosis proteins 1 and 2 complex (TSC1/2). RESULTS: In contrast to the previous studies using Tsc1 knockout mouse embryonic fibroblasts (MEF) cells, we demonstrated evidence that TSC1 deficient macrophages exhibited enhanced basal and mycobacterial infection-induced autophagy via AMPKα-dependent phosphorylation of ULK1 (Ser555)...
2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27216037/molecular-characterization-of-protein-kinase-c-delta-pkc%C3%AE-smac-interactions
#20
Christian Holmgren, Louise Cornmark, Gry Kalstad Lønne, Katarzyna Chmielarska Masoumi, Christer Larsson
BACKGROUND: Protein kinase C δ (PKCδ) is known to be an important regulator of apoptosis, having mainly pro- but also anti-apoptotic effects depending on context. In a previous study, we found that PKCδ interacts with the pro-apoptotic protein Smac. Smac facilitates apoptosis by suppressing inhibitor of apoptosis proteins (IAPs). We previously established that the PKCδ-Smac complex dissociates during induction of apoptosis indicating a functional importance. Because the knowledge on the molecular determinants of the interaction is limited, we aimed at characterizing the interactions between PKCδ and Smac...
2016: BMC Biochemistry
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