journal
MENU ▼
Read by QxMD icon Read
search

BMC Biochemistry

journal
https://www.readbyqxmd.com/read/29216817/characterization-of-sulfhydryl-oxidase-from-aspergillus-tubingensis
#1
Outi Nivala, Greta Faccio, Mikko Arvas, Perttu Permi, Johanna Buchert, Kristiina Kruus, Maija-Liisa Mattinen
BACKGROUND: Despite of the presence of sulfhydryl oxidases (SOXs) in the secretomes of industrially relevant organisms and their many potential applications, only few of these enzymes have been biochemically characterized. In addition, basic functions of most of the SOX enzymes reported so far are not fully understood. In particular, the physiological role of secreted fungal SOXs is unclear. RESULTS: The recently identified SOX from Aspergillus tubingensis (AtSOX) was produced, purified and characterized in the present work...
December 8, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/29166856/glycyl-alanyl-histidine-protects-pc12-cells-against-hydrogen-peroxide-toxicity
#2
Hideki Shimura, Ryota Tanaka, Yoshiaki Shimada, Kazuo Yamashiro, Nobutaka Hattori, Takao Urabe
BACKGROUND: Peptides with cytoprotective functions, including antioxidants and anti-infectives, could be useful therapeutics. Carnosine, β-alanine-histidine, is a dipeptide with anti-oxidant properties. Tripeptides of Ala-His-Lys, Pro-His-His, or Tyr-His-Tyr are also of interest in this respect. RESULTS: We synthesized several histidine-containing peptides including glycine or alanine, and tested their cytoprotective effects on hydrogen peroxide toxicity for PC12 cells...
November 22, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28893174/antiproliferative-factor-apf-binds-specifically-to-sites-within-the-cytoskeleton-associated-protein-4-ckap4-extracellular-domain
#3
Burzin Chavda, Jun Ling, Thomas Majernick, Sonia Lobo Planey
BACKGROUND: Antiproliferative factor (APF) is a sialoglycopeptide elevated in the urine of patients with interstitial cystitis-a chronic, painful bladder disease. APF inhibits the proliferation of normal bladder epithelial cells and cancer cells in vitro, presumably by binding to its cellular receptor, cytoskeleton associated-protein 4 (CKAP4); however, the biophysical interaction of APF with CKAP4 has not been characterized previously. In this study, we used surface plasmon resonance (SPR) to explore the binding kinetics of the interaction of APF and as-APF (a desialylated APF analogue with full activity) to CKAP4...
September 11, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28724347/use-of-a-special-brazilian-red-light-emitting-railroad-worm-luciferase-in-bioassays-of-nek7-protein-kinase-and-creatine-kinase
#4
Arina Marina Perez, Bruno Aquino, Vadim Viviani, Jörg Kobarg
BACKGROUND: Luciferases, enzymes that catalyze bioluminescent reactions in different organisms, have been extensively used for bioanalytical purposes. The most well studied bioluminescent system is that of firefly and other beetles, which depends on a luciferase, a benzothiazolic luciferin and ATP, and it is being widely used as a bioanalytical reagent to quantify ATP. Protein kinases are proteins that modify other proteins by transferring phosphate groups from a nucleoside triphosphate, usually ATP...
July 19, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28701157/dacin-one-metalloproteinase-from-deinagkistrodon-acutus-venom-inhibiting-contraction-of-mouse-ileum-muscle
#5
Bin Zhou, Gang Liu, Qiyi He, Bo Li, Xiaodong Yu
BACKGROUND: Mice were bitten by five-pace vipers (Deinagkistrodon acutus), and then envenomed. It was well-known that the snake venom mainly disturbed the blood homeostasis of the envenomed victims. Ocassionally, we found that the venom of D. acutus could inhibit the contraction tension of mouse ileum, so in this study we aimed to identify the active component inhibiting the contraction tension of mouse ileum in the snake venom. RESULTS: The active component inhibiting the contraction tension of mouse ileum, designated as Dacin, was isolated from D...
July 12, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28569147/serendipitous-discovery-of-light-induced-in-situ-formation-of-an-azo-bridged-dimeric-sulfonated-naphthol-as-a-potent-ptp1b-inhibitor
#6
Robert D Bongard, Michael Lepley, Khushabu Thakur, Marat R Talipov, Jaladhi Nayak, Rachel A Jones Lipinski, Chris Bohl, Noreena Sweeney, Ramani Ramchandran, Rajendra Rathore, Daniel S Sem
BACKGROUND: Protein tyrosine phosphatases (PTPs) like dual specificity phosphatase 5 (DUSP5) and protein tyrosine phosphatase 1B (PTP1B) are drug targets for diseases that include cancer, diabetes, and vascular disorders such as hemangiomas. The PTPs are also known to be notoriously difficult targets for designing inihibitors that become viable drug leads. Therefore, the pipeline for approved drugs in this class is minimal. Furthermore, drug screening for targets like PTPs often produce false positive and false negative results...
May 31, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28545389/structural-insight-into-the-inactivation-of-mycobacterium-tuberculosis-non-classical-transpeptidase-ldtmt2-by-biapenem-and-tebipenem
#7
Mario A Bianchet, Ying H Pan, Leighanne A Brammer Basta, Harry Saavedra, Evan P Lloyd, Pankaj Kumar, Rohini Mattoo, Craig A Townsend, Gyanu Lamichhane
BACKGROUND: The carbapenem subclass of β-lactams is among the most potent antibiotics available today. Emerging evidence shows that, unlike other subclasses of β-lactams, carbapenems bind to and inhibit non-classical transpeptidases (L,D-transpeptidases) that generate 3 → 3 linkages in bacterial peptidoglycan. The carbapenems biapenem and tebipenem exhibit therapeutically valuable potencies against Mycobacterium tuberculosis (Mtb). RESULTS: Here, we report the X-ray crystal structures of Mtb L,D-transpeptidase-2 (LdtMt2) complexed with biapenem or tebipenem...
May 25, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28545388/chemical-and-structural-characterization-of-%C3%AE-n-acetylgalactosaminidase-i-and-ii-from-starfish-asterina-amurensis
#8
Md Harun-Or Rashid, Golam Sadik, Ahm Khurshid Alam, Toshihisa Tanaka
BACKGROUND: The marine invertebrate starfish was found to contain a novel α-N-acetylgalactosaminidase, α-GalNAcase II, which catalyzes removal of terminal α-N-acetylgalactosamine (α-GalNAc), in addition to a typical α-N-acetylgalactosaminidase, α-GalNAcase I, which catalyzes removal of terminal α-N-acetylgalactosamine (α-GalNAc) and, to a lesser extent, galactose. The interrelationship between α-GalNAcase I and α-GalNAcase II and the molecular basis of their differences in substrate specificity remain unknown...
May 25, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28511672/crmapk3-regulates-the-expression-of-iron-deficiency-responsive-genes-in-chlamydomonas-reinhardtii
#9
Xiaowen Fei, Junmei Yu, Yajun Li, Xiaodong Deng
BACKGROUND: Under iron-deficient conditions, Chlamydomonas exhibits high affinity for iron absorption. Nevertheless, the response, transmission, and regulation of downstream gene expression in algae cells have not to be investigated. Considering that the MAPK pathway is essential for abiotic stress responses, we determined whether this pathway is involved in iron deficiency signal transduction in Chlamydomonas. RESULTS: Arabidopsis MAPK gene sequences were used as entry data to search for homologous genes in Chlamydomonas reinhardtii genome database to investigate the functions of mitogen-activated protein kinase (MAPK) gene family in C...
May 16, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28511668/structural-similarities-and-functional-differences-clarify-evolutionary-relationships-between-trna-healing-enzymes-and-the-myelin-enzyme-cnpase
#10
Gopinath Muruganandam, Arne Raasakka, Matti Myllykoski, Inari Kursula, Petri Kursula
BACKGROUND: Eukaryotic tRNA splicing is an essential process in the transformation of a primary tRNA transcript into a mature functional tRNA molecule. 5'-phosphate ligation involves two steps: a healing reaction catalyzed by polynucleotide kinase (PNK) in association with cyclic phosphodiesterase (CPDase), and a sealing reaction catalyzed by an RNA ligase. The enzymes that catalyze tRNA healing in yeast and higher eukaryotes are homologous to the members of the 2H phosphoesterase superfamily, in particular to the vertebrate myelin enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)...
May 16, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28320308/binding-of-smoothelin-like-1-to-tropomyosin-and-calmodulin-is-mutually-exclusive-and-regulated-by-phosphorylation
#11
Annegret Ulke-Lemée, David Hao Sun, Hiroaki Ishida, Hans J Vogel, Justin A MacDonald
BACKGROUND: The smoothelin-like 1 protein (SMTNL1) can associate with tropomyosin (Tpm) and calmodulin (CaM), two proteins essential to the smooth muscle contractile process. SMTNL1 is phosphorylated at Ser301 by protein kinase A during calcium desensitization in smooth muscle, yet the effect of SMTNL1 phosphorylation on Tpm- and CaM-binding has yet to be investigated. RESULTS: Using pull down studies with Tpm-Sepharose and CaM-Sepharose, we examined the interplay between Tpm binding, CaM binding, phosphorylation of SMTNL1 and calcium concentration...
March 21, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28302049/increased-enzymatic-hydrolysis-of-sugarcane-bagasse-by-a-novel-glucose-and-xylose-stimulated-%C3%AE-glucosidase-from-anoxybacillus-flavithermus-subsp-yunnanensis-e13-t
#12
Yang Liu, Rui Li, Jing Wang, Xiaohan Zhang, Rong Jia, Yi Gao, Hui Peng
BACKGROUND: β-Glucosidase is claimed as a key enzyme in cellulose hydrolysis. The cellulosic fibers are usually entrapped with hemicelluloses containing xylose. So there is ongoing interest in searching for glucose- and xylose-stimulated β-glucosidases to increase the efficiency of hydrolysis of cellulosic biomass. RESULTS: A thermostable β-glucosidase gene (Bglp) was cloned from Anoxybacillus flavithermus subsp. yunnanensis E13(T) and characterized. Optimal enzyme activity was observed at 60 °C and pH 7...
March 16, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28264651/interactions-of-histatin-3-and-histatin-5-with-actin
#13
Edna Blotnick, Asaf Sol, Gilad Bachrach, Andras Muhlrad
BACKGROUND: Histatins are histidine rich polypeptides produced in the parotid and submandibular gland and secreted into the saliva. Histatin-3 and -5 are the most important polycationic histatins. They possess antimicrobial activity against fungi such as Candida albicans. Histatin-5 has a higher antifungal activity than histatin-3 while histatin-3 is mostly involved in wound healing in the oral cavity. We found that these histatins, like other polycationic peptides and proteins, such as LL-37, lysozyme and histones, interact with extracellular actin...
March 6, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28264645/systematic-substitutions-at-blip-position-50-result-in-changes-in-binding-specificity-for-class-a-%C3%AE-lactamases
#14
Carolyn J Adamski, Timothy Palzkill
BACKGROUND: The production of β-lactamases by bacteria is the most common mechanism of resistance to the widely prescribed β-lactam antibiotics. β-lactamase inhibitory protein (BLIP) competitively inhibits class A β-lactamases via two binding loops that occlude the active site. It has been shown that BLIP Tyr50 is a specificity determinant in that substitutions at this position result in large differential changes in the relative affinity of BLIP for class A β-lactamases. RESULTS: In this study, the effect of systematic substitutions at BLIP position 50 on binding to class A β-lactamases was examined to further explore the role of BLIP Tyr50 in modulating specificity...
March 6, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28118841/copper-chelation-and-interleukin-6-proinflammatory-cytokine-effects-on-expression-of-different-proteins-involved-in-iron-metabolism-in-hepg2-cell-line
#15
Luca Marco Di Bella, Roberto Alampi, Flavia Biundo, Giovanni Toscano, Maria Rosa Felice
BACKGROUND: In vertebrates, there is an intimate relationship between copper and iron homeostasis. Copper deficiency, which leads to a defect in ceruloplasmin enzymatic activity, has a strong effect on iron homeostasis resulting in cellular iron retention. Much is known about the mechanisms underlying cellular iron retention under "normal" conditions, however, less is known about the effect of copper deficiency during inflammation. RESULTS: We show that copper deficiency and the inflammatory cytokine interleukin-6 have different effects on the expression of proteins involved in iron and copper metabolism such as the soluble and glycosylphosphtidylinositol anchored forms of ceruloplasmin, hepcidin, ferroportin1, transferrin receptor1, divalent metal transporter1 and H-ferritin subunit...
January 24, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/27905872/knockdown-of-ggct-inhibits-cell-proliferation-and-induces-late-apoptosis-in-human-gastric-cancer
#16
Wenjie Zhang, Lei Chen, Honggang Xiang, Chunhua Hu, Weibin Shi, Ping Dong, Wenjie Lv
BACKGROUND: Gamma glutamylcyclotransferase (GGCT) has been proved to be involved in various cancers, but the biological function of GGCT in gastric cancer is still largely unknown. METHODS: The expression level of GGCT was evaluated by informatics analyses based on the Oncomine database. GGCT gene was then effectively knocked down via lentivirus mediated short hairpin RNA (shRNA) system. Then a series of functional assays, including MTT, colony formation and flow cytometry analysis were conducted on gastric cancer cells following GGCT knockdown...
December 1, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27716023/identification-of-replication-dependent-and-replication-independent-linker-histone-complexes-tpr-specifically-promotes-replication-dependent-linker-histone-stability
#17
Pei Zhang, Owen E Branson, Michael A Freitas, Mark R Parthun
BACKGROUND: There are 11 variants of linker histone H1 in mammalian cells. Beyond their shared abilities to stabilize and condense chromatin, the H1 variants have been found to have non-redundant functions, the mechanisms of which are not fully understood. Like core histones, there are both replication-dependent and replication-independent linker histone variants. The histone chaperones and other factors that regulate linker histone dynamics in the cell are largely unknown. In particular, it is not known whether replication-dependent and replication-independent linker histones interact with distinct or common sets of proteins...
October 1, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27474398/avoiding-false-discovery-in-biomarker-research
#18
Pranali Patel, Uros Kuzmanov, Seema Mital
BACKGROUND: Human tyrosine-protein phosphatase non-receptor type substrate 1α (SIRPA) is a surface marker identified in cardiomyocytes differentiated from human embryonic stem cells. Our objective was to determine if circulating SIRPA levels can serve as a biomarker of cardiac injury in children undergoing open heart surgery. RESULTS: Paired pre- and post-operative serum samples from 48 pediatric patients undergoing open heart surgery and from 6 pediatric patients undergoing non-cardiac surgery (controls) were tested for SIRPA protein levels using commercially available SIRPA ELISA kits from two manufacturers...
July 30, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27455956/computational-studies-of-human-class-v-alcohol-dehydrogenase-the-odd-sibling
#19
Linus J Östberg, Bengt Persson, Jan-Olov Höög
BACKGROUND: All known attempts to isolate and characterize mammalian class V alcohol dehydrogenase (class V ADH), a member of the large ADH protein family, at the protein level have failed. This indicates that the class V ADH protein is not stable in a non-cellular environment, which is in contrast to all other human ADH enzymes. In this report we present evidence, supported with results from computational analyses performed in combination with earlier in vitro studies, why this ADH behaves in an atypical way...
July 25, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/27393145/effect-of-mutations-to-amino-acid-a301-and-f361-in-thermostability-and-catalytic-activity-of-the-%C3%AE-galactosidase-from-bacillus-subtilis-vtcc-dvn-12-01
#20
Thao Thi Nguyen, Hanh Van Vu, Nhung Thi Hong Nguyen, Tuyen Thi Do, Thanh Sy Le Nguyen
BACKGROUND: Beta-galactosidase (EC 3.2.1.23), a commercially important enzyme, catalyses the hydrolysis of β-1,3- and β-1,4-galactosyl bonds of polymer or oligosaccharidesas well as transglycosylation of β-galactopyranosides. Due to catalytic properties; β-galactosidase might be useful in the milk industry to hydrolyze lactose and produce prebiotic GOS. The purpose of this study is to characterize β-galactosidase mutants from B. subtilis. RESULTS: Using error prone rolling circle amplification (epRCA) to characterize some random mutants of the β-galactosidase (LacA) from B...
July 8, 2016: BMC Biochemistry
journal
journal
38191
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"