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Journal of Cellular and Molecular Medicine

Miaoda Shen, Ronghuan Wu, Rilong Jin, Jun Pan, Fang Guo, Zhuoyang Li, Xiangjin Lin, Sanzhong Xu
Osteoporosis is a severe skeletal disorder. Patients have a low bone mineral density and bone structural deterioration. Mounting lines of evidence suggest that inappropriate apoptosis of osteoblasts/osteocytes leads to maladaptive bone remodelling in osteoporosis. It has been suggested that transplantation of stem cells, including mesenchymal stem cells, may alter the trajectory of bone remoulding and mitigate osteoporosis in animal models. However, stem cells needed to be carefully stored and characterized before usage...
May 16, 2018: Journal of Cellular and Molecular Medicine
Rong Wang, Chun-Fang Wang, Hai-Mei Qin, Yu-Lan Lu, Gui-Jiang Wei, Hua-Tuo Huang, Yang Xiang, Jun-Li Wang, Yan Lan, Ye-Sheng Wei
The aim of this study was to investigate the association of genetic polymorphisms in the promoter region of miR-17-92 with systemic lupus erythematosus (SLE). The gene polymorphism was analysed using SNaPshot in 312 SLE patients and 396 controls. Relative expression of miR-17-92 was measured by quantitative real-time PCR. Association was found between rs9515692 and a decreased risk of SLE (CT vs CC: OR = 0.65, 95%CI, 0.46-0.92, P = .014; CT+TT vs CC: OR = 0.64, 95%CI, 0.46-0.90, P = .009; T vs C: OR = 0.69, 95%CI, 0...
May 16, 2018: Journal of Cellular and Molecular Medicine
Defeng Li, Meifeng Yang, Aijun Liao, Bing Zeng, Diqun Liu, Yuhong Yao, Guangsheng Hu, Xuanmin Chen, Zhiqiang Feng, Yanlei Du, Youlian Zhou, Jie He, Yuqiang Nie
Long non-coding RNAs (lncRNAs) are important regulators of many cellular processes, and their aberrant expression and/or function is associated with many different diseases, including cancer. However, the identification of functional lncRNAs in gastric cancer is still a challenge. In this study, we describe a novel functional lncRNA, linc00483, that is upregulated and associated with tumorigenesis, tumour size, metastasis and poor prognosis in gastric cancer. In our study, linc00483 promoted gastric cancer cell proliferation, invasiveness and metastasis in vitro and in vivo...
May 15, 2018: Journal of Cellular and Molecular Medicine
Meixiao Zhan, Ke He, Jing Xiao, Fei Liu, Haihe Wang, Zhenglin Xia, Xiaopeng Duan, Rui Huang, Yong Li, Xu He, Hua Yin, Guoan Xiang, Ligong Lu
Accumulating studies supported that lncRNAs played important roles in tumorigenesis. LncRNA HOXA11-AS was a novel lncRNA that has been proved to involved in several tumours. However, the role of HOXA11-AS in the development of hepatocellular carcinoma (HCC) remains to be explained. In our study, we showed that HOXA11-AS expression was up-regulated in the HCC tissues, and the higher expression of HOXA11-AS was associated with the advanced stage in the HCC samples. In addition, we indicated that the expression of HOXA11-AS was up-regulated in HCC cell lines (Hep3B, SMMC-7721, MHCC97-H and BEL-7402) compared with normal liver cell lines (HL-7702)...
May 15, 2018: Journal of Cellular and Molecular Medicine
Liyuan Huang, Fangfang Song, Hualing Sun, Lu Zhang, Cui Huang
Iroquois homeobox gene 5 (Irx5) is a highly conserved member of the Iroquois homeobox gene family. Members of this family play distinct and overlapping roles in normal embryonic cell patterning and development of malignancies. In this study, we observed that IRX5 was abnormally abundant in tongue squamous cell carcinoma (TSCC) tissues and cell lines. We used gain- and loss-of-function methods to overexpress and knockdown IRX5 expression in the TSCC cell line CAL27. Our results elucidated that elevated levels of IRX5 promoted proliferation, migration and invasion of TSCC cells, whereas stable or transient knockdown of IRX5 expression suppressed TSCC cell proliferation, migration and invasion...
May 15, 2018: Journal of Cellular and Molecular Medicine
Wei Yu, Yuming Ma, Sharmila Shankar, Rakesh K Srivastava
The incidence of pancreatic cancer is on the rise. Risk factors for pancreatic cancer include alcohol toxicity and metabolic conditions such as obesity, hypertension, dyslipidaemia, insulin resistance and type 2 diabetes. However, the molecular mechanism by which chronic alcohol consumption contributes to pancreatic cancer is not well understood. The purpose of the study was to demonstrate the effects of long-term chronic ethanol exposure on the transformation of human pancreatic normal ductal epithelial (HPNE) cells...
May 15, 2018: Journal of Cellular and Molecular Medicine
Guan-Sheng Liu, George Gardner, George Adly, Min Jiang, Wen-Feng Cai, Chi Keung Lam, Fawzi Alogaili, Nathan Robbins, Jack Rubinstein, Evangelia G Kranias
Heat shock protein 20 (Hsp20) has been shown to be a critical regulator of cardiomyocyte survival upon cardiac stress. In this study, we investigated the functional significance of a novel human Hsp20 mutation (S10F) in peripartum cardiomyopathy. Previous findings showed that cardiac-specific overexpression of this mutant were associated with reduced autophagy, left ventricular dysfunction and early death in male mice. However, this study indicates that females have normal function with no alterations in autophagy but died within a week after 1-4 pregnancies...
May 15, 2018: Journal of Cellular and Molecular Medicine
Dina Stroopinsky, Hasan Rajabi, Myrna Nahas, Jacalyn Rosenblatt, Maryam Rahimian, Athalia Pyzer, Ashujit Tagde, Akriti Kharbanda, Salvia Jain, Turner Kufe, Rebecca K Leaf, Eleni Anastasiadou, Michal Bar-Natan, Shira Orr, Maxwell D Coll, Kristen Palmer, Adam Ephraim, Leandra Cole, Abigail Washington, Donald Kufe, David Avigan
Acute myeloid leukaemia (AML) is an aggressive haematological malignancy with an unmet need for improved therapies. Responses to standard cytotoxic therapy in AML are often transient because of the emergence of chemotherapy-resistant disease. The MUC1-C oncoprotein governs critical pathways of tumorigenesis, including self-renewal and survival, and is aberrantly expressed in AML blasts and leukaemia stem cells (LSCs). However, a role for MUC1-C in linking leukaemogenesis and resistance to treatment has not been described...
May 15, 2018: Journal of Cellular and Molecular Medicine
Hua Wang, Bin Huang, Bai Mou Li, Kai Yuan Cao, Chen Qiang Mo, Shuang Jian Jiang, Jin Cheng Pan, Zong Ren Wang, Huan Yi Lin, Dao Hu Wang, Shao Peng Qiu
The zinc finger E-box-binding homeobox 1 (ZEB1) induced the epithelial-mesenchymal transition (EMT) and altered ZEB1 expression could lead to aggressive and cancer stem cell (CSC) phenotypes in various cancers. Tissue specimens from 96 prostate cancer patients were collected for immunohistochemistry and CD34/periodic acid-Schiff double staining. Prostate cancer cells were subjected to ZEB1 knockdown or overexpression and assessment of the effects on vasculogenic mimicry formation in vitro and in vivo. The underlying molecular events of ZEB1-induced vasculogenic mimicry formation in prostate cancer were then explored...
May 12, 2018: Journal of Cellular and Molecular Medicine
Jan W Robering, Annika Weigand, Romy Pfuhlmann, Raymund E Horch, Justus P Beier, Anja M Boos
Lymphatic metastasis is one of the main prognostic factors concerning long-term survival of cancer patients. In this regard, the molecular mechanisms of lymphangiogenesis are still rarely explored. Also, the interactions between stem cells and lymphatic endothelial cells (LEC) in humans have not been well examined. Therefore, the main objective of this study was to assess the interactions between mesenchymal stem cells (MSC) and LEC using in vitro angiogenesis assays. Juvenile LEC were stimulated with VEGF-C, bFGF, MSC-conditioned medium (MSC-CM) or by co-culture with MSC...
May 11, 2018: Journal of Cellular and Molecular Medicine
Yuanyuan Wang, Jiahui Chen, Man Zhang, Wenwen Zhang, Min Li, Wenqiao Zang, Ziming Dong, Guoqiang Zhao
Human DNA polymerase β (polβ) is a small, monomeric protein essential for short-patch base excision repair (BER). polβ plays an important role in the regulation of chemotherapy sensitivity in tumour cells. In this study, we determined that the expression levels of polβ mRNA and miR-149 in tumour tissues were significantly higher than in adjacent non-tumour tissues. We also found that the expression level of miR-149 in EC tumour tissues was inverse to that of polβ expression. Bioinformatics analysis and dual-luciferase reporter assay predicted that miR-149 negatively regulates polβ expression by directly binding to its 3'UTR...
May 4, 2018: Journal of Cellular and Molecular Medicine
Ji-Chang Wang, Xin Sun, Qiang Ma, Gui-Feng Fu, Long-Long Cong, Hong Zhang, De-Fu Fan, Jun Feng, Shao-Ying Lu, Jian-Lin Liu, Guang-Yue Li, Pei-Jun Liu
Beneficial effects of metformin on cancer risk and mortality have been proved by epidemiological and clinical studies, thus attracting research interest in elucidating the underlying mechanisms. Recently, tumour-associated macrophages (TAMs) appeared to be implicated in metformin-induced antitumour activities. However, how metformin inhibits TAMs-induced tumour progression remains ill-defined. Here, we report that metformin-induced antitumour and anti-angiogenic activities were not or only partially contributed by its direct inhibition of functions of tumour and endothelial cells...
May 4, 2018: Journal of Cellular and Molecular Medicine
Dongfang Tang, Dandan Zhao, Yun Wu, Ruyong Yao, Lin Zhou, Liming Lu, Wen Gao, Yifeng Sun
It is less known about miRNA3127-5p induced up-regulation of PD-L1, immune escape and drug resistance caused by increased PD-L1 in lung cancer. In this study, lentivirus was transduced into lung cancer cells, and quantitative PCR and Western blot were used to detect the expression of PD-L1. Then immunofluorescence assay was applied to detect autophagy, finally we explored the relationship between PD-L1 expressions and chemoresistance in patients. As a result, we found that microRNA-3127-5p promotes pSTAT3 to induce the expression of PD-L1; microRNA-3127-5p promotes STAT3 phosphorylation through suppressing autophagy, and autophagy could retaine pSTAT3 into the nucleus in miRNA-3127-5p knocked cells, and immune escape induced by elevated level of PD-L1 results in chemoresistance of lung cancer...
May 4, 2018: Journal of Cellular and Molecular Medicine
Yin-Quan Chen, Hsin-Yi Lan, Yi-Chang Wu, Wen-Hao Yang, Arthur Chiou, Muh-Hwa Yang
The biological impact and signalling of epithelial-mesenchymal transition (EMT) during cancer metastasis has been established. However, the changes in biophysical properties of cancer cells undergoing EMT remain elusive. Here, we measured, via video particle tracking microrheology, the intracellular stiffness of head and neck cancer cell lines with distinct EMT phenotypes. We also examined cells migration and invasiveness in different extracellular matrix architectures and EMT-related signalling in these cell lines...
May 4, 2018: Journal of Cellular and Molecular Medicine
Ketao Zhang, Siyao Che, Chuzhi Pan, Zheng Su, Shangyou Zheng, Shanglin Yang, Huayao Zhang, Wenda Li, Weidong Wang, Jianping Liu
The cell surface antigen CD90 has recently been established as a promising marker for liver cancer stem cells. This study aimed to investigate potential implications of SHH/Gli signalling in CD90+ liver cancer stem cells. Correlation of the expression of SHH signalling components and CD90 in liver cancer cells and clinical tissues, as well as in enriched CD90+ liver cancer stem cells and the TCGA database, were analysed by quantitative RT-PCR, Western blotting and flow cytometry. Functional analysis was conducted by siRNA-mediated CD90, Gli1 and Gli3 gene knockdown, SHH treatment and application of the JAK2 inhibitor AZD1480 and IL6 neutralizing antibody in CD90+ liver cancer stem cells, followed by cell proliferation, migration, sphere formation and tumorigenicity assays...
May 2, 2018: Journal of Cellular and Molecular Medicine
Pavel F Pavlov, Birgit Hutter-Paier, Daniel Havas, Manfred Windisch, Bengt Winblad
Mitochondrial dysfunction is an early feature of Alzheimer's disease (AD) and may play an important role in the pathogenesis of disease. It has been shown that amyloid beta peptide (Aβ) and amyloid precursor protein (APP) interact with mitochondria contributing to the mitochondrial dysfunction in AD. Prevention of abnormal protein targeting to mitochondria can protect normal mitochondrial function, increase neuronal survival and at the end, ameliorate symptoms of AD and other neurodegenerative disorders. First steps of mitochondrial protein import are coordinated by molecular chaperones Hsp70 and Hsp90 that bind to the newly synthesized mitochondria-destined proteins and deliver them to the protein import receptors on the surface of organelle...
April 27, 2018: Journal of Cellular and Molecular Medicine
Lei Chen, Baoping Yu
A novel type of interstitial tissue cells in the biliary tree termed telocytes (TCs), formerly known as interstitial Cajal-like cells (ICLCs), exhibits very particular features which unequivocally distinguish these cells from interstitial cells of Cajal (ICCs) and other interstitial cell types. Current research substantiates the existence of TCs and ICCs in the biliary system (gallbladder, extrahepatic bile duct, cystic duct, common bile duct and sphincter of Oddi). Here, we review the distribution, morphology and ultrastructure of TCs and ICCs in the biliary tree, with emphasis on their presumptive roles in physiological and pathophysiological processes...
April 26, 2018: Journal of Cellular and Molecular Medicine
Jingjing Liu, Jiajun Wang, Yongxi Song, Bin Ma, Junlong Luo, Zhongran Ni, Peng Gao, Jingxu Sun, Junhua Zhao, Xiaowan Chen, Zhenning Wang
Early detection is vital for prolonging 5-year survival for patients with gastric cancer (GC). Numerous studies indicate that circulating long non-coding RNAs (lncRNAs) can be used to diagnose malignant tumours. This study aimed to investigate the capacity of novel lncRNAs for diagnosing GC. A lncRNA microarray assay was used to screen differentially expressed lncRNAs between plasma of patients with GC and healthy controls. Plasma samples from 100 patients with healthy controls were used to construct a multiple-gene panel...
April 26, 2018: Journal of Cellular and Molecular Medicine
Pengjiao Zeng, Zhihua Guo, Xuan Zeng, Cui Hao, Yiran Zhang, Meng Zhang, Yong Liu, Hui Li, Juan Li, Lijuan Zhang
Ganoderma lucidum is an edible medicinal mushroom known as "Lingzhi" in China and "Reishi or Manetake" in Japan. It is a highly prized vitality-enhancing herb for more than 2000 years. G. lucidum polysaccharide (GLPS) has been identified as one of the major bioactive components and developed into a drug named "Ji 731 Injection" in China since 1973. The large-scale production of the drug began in 1985 and approved by the Chinese FDA as "Polysaccharidum of G. lucidum Karst Injection" (Ling Bao Duo Tang Zhu She Ye) in 2000, which is applied intramuscularly...
April 24, 2018: Journal of Cellular and Molecular Medicine
Li-Ling Yue, Fu-Chao Wang, Ming-Long Zhang, Dan Liu, Ping Chen, Qing-Bu Mei, Peng-Hui Li, Hong-Ming Pan, Li-Hong Zheng
We tested the hypothesis that genetic variation in ATM and BMI-1 genes can alter the risk of breast cancer through genotyping 6 variants among 524 breast cancer cases and 518 cancer-free controls of Han nationality. This was an observational, hospital-based, case-control association study. Analyses of single variant, linkage, haplotype, interaction and nomogram were performed. Risk was expressed as odds ratio (OR) and 95% confidence interval (CI). All studied variants were in the Hardy-Weinberg equilibrium and were not linked...
April 24, 2018: Journal of Cellular and Molecular Medicine
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