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Journal of Cellular and Molecular Medicine

Massimo Dal Monte, Maurizio Cammalleri, Valeria Pecci, Monica Carmosino, Giuseppe Procino, Alessandro Pini, Mario De Rosa, Vincenzo Pavone, Maria Svelto, Paola Bagnoli
The urokinase-type plasminogen activator (uPA) receptor (uPAR) participates to the mechanisms causing renal damage in response to hyperglycaemia. The main function of uPAR in podocytes (as well as soluble uPAR -(s)uPAR- from circulation) is to regulate podocyte function through αvβ3 integrin/Rac-1. We addressed the question of whether blocking the uPAR pathway with the small peptide UPARANT, which inhibits uPAR binding to the formyl peptide receptors (FPRs) can improve kidney lesions in a rat model of streptozotocin (STZ)-induced diabetes...
November 13, 2018: Journal of Cellular and Molecular Medicine
Won-Jun Jang, Sung Keun Jung, Tam Thuy Lu Vo, Chul-Ho Jeong
The concept of drug repositioning has recently received considerable attention in the field of oncology. In the present study, we propose that paroxetine can be used as a potent anticancer drug. Paroxetine, one of the selective serotonin reuptake inhibitors (SSRIs), has been widely prescribed for the treatment of depression and anxiety disorders. Recently, SSRIs have been reported to have anticancer activity in various types of cancer cells; however, the underlying mechanisms of their action are not yet known...
November 13, 2018: Journal of Cellular and Molecular Medicine
Qianhui Chen, Xuxue Guo, Nishan Deng, Linlin Liu, Shuo Chen, Ailing Wang, Ruiyun Li, Yi Huang, Xuhong Ding, Hongying Yu, Suping Hu, Hanxiang Nie
Our previous study showed that intraperitoneal injection of α-galactosylceramide (α-GalCer) has the ability to activate lung iNKT cells, but α-GalCer-activated iNKT cells do not result in airway inflammation in wild-type (WT) mice. Many studies showed that iNKT cells had the capacity to induce Treg cells, which gave rise to peripheral tolerance. Therefore, we examined the influence of intraperitoneal administration of α-GalCer on the expansion and suppressive activity of lung Treg cells using iNKT cell-knockout mice and co-culture experiments in vitro...
November 13, 2018: Journal of Cellular and Molecular Medicine
Chenchen Feng, Chao Song, Ziyu Ning, Bo Ai, Qiuyu Wang, Yong Xu, Meng Li, Xuefeng Bai, Jianmei Zhao, Yuejuan Liu, Xuecang Li, Jian Zhang, Chunquan Li
Competing endogenous RNAs (ceRNAs) represent a novel mechanism of gene regulation that may mediate key subpathway regions and contribute to the altered activities of pathways. However, the classical methods used to identify pathways fail to specifically consider ceRNAs within the pathways and key regions impacted by them. We proposed a powerful strategy named ce-Subpathway for the identification of ceRNA-mediated functional subpathways. It provided an effective level of pathway analysis via integrating ceRNAs, differentially expressed (DE) genes and their key regions within the given pathways...
November 12, 2018: Journal of Cellular and Molecular Medicine
Yong-Hua Zhao, Nai-Wei Liu, Chien-Chih Ke, Bo-Wen Liu, Yi-An Chen, Cheng Luo, Qian Zhang, Zhen-Yan Xia, Ren-Shyan Liu
The remodelling of structural and functional neurovascular unit (NVU) becomes a central therapeutic strategy after cerebral ischaemic stroke. In the present study, we investigated the effect of combined therapy of sodium ferulate (SF), n-butylidenephthalide (BP) and adipose-derived stromal cells (ADSCs) to ameliorate the injured NVU in the photochemically induced thrombotic stroke in rats. After solely or combined treatment, the neovascularization, activation of astrocytes, neurogenesis, expressions of vascular endothelial growth factor (VEGF) and claudin-5 were assessed by immunohistochemical or immunofluorescence staining...
November 12, 2018: Journal of Cellular and Molecular Medicine
Binbin Yu, Yan Zhang, Kailiu Wu, Lizhen Wang, Yingying Jiang, Wantao Chen, Ming Yan
CD147/basigin (BSG) is highly upregulated in many types of cancer, our previous study has found that CD147/BSG is highly expressed in head and neck squamous cell carcinoma (HNSCC) stem cells, but its role in HNSCC and the underlying mechanism is still unknown. In this study, we investigated the role of CD147 in the progression of HNSCC. Real-time PCR, western blot and immunohistochemistry were used to detect the expression of CD147 in total 189 HNSCC tissues in compared with normal tissues. In addition, we used proliferation, colony formation, cell cycle and apoptosis, migration and invasion as well as wound-healing assay to determine the biological roles of CD147 in HNSCC...
November 12, 2018: Journal of Cellular and Molecular Medicine
Xiuying Shi, Yang Zhao, Chuifeng Fan
Our previous work showed that Zbed3 is overexpressed in nonsmall cell lung cancer and that down-regulation of Zbed3 inhibited β-catenin expression and cancer cell proliferation and invasiveness. Here, we investigated Zbed3's ability to promote lung cancer cell proliferation and invasion and the involvement of the Axin/TPC/glycogen synthase kinase 3β (Gsk-3β) complex to the response. Coimmunoprecipitation assays showed that wild-type Zbed3 bound to Axin but a Zbed3 mutant lacking the Axin binding site did not...
November 12, 2018: Journal of Cellular and Molecular Medicine
Anabel Fernández-Iglesias, Martí Ortega-Ribera, Sergi Guixé-Muntet, Jordi Gracia-Sancho
Liver cells isolated from pre-clinical models are essential tools for studying liver (patho)physiology, and also for screening new therapeutic options. We aimed at developing a new antibody-free isolation method able to obtain the four main hepatic cell types (hepatocytes, liver sinusoidal endothelial cells [LSEC], hepatic macrophages [HMΦ] and hepatic stellate cells [HSC]) from a single rat liver. Control and cirrhotic (CCl4 and TAA) rat livers (n = 6) were perfused, digested with collagenase and mechanically disaggregated obtaining a multicellular suspension...
November 12, 2018: Journal of Cellular and Molecular Medicine
Wei Wang, Yang Wang, Mingxing Liu, Yifan Zhang, Tao Yang, Dongsheng Li, Yinpeng Huang, Qing Li, Guang Bai, Linlin Shi
Hepatocellular carcinoma (HCC) is a high incidence and mortality malignant tumour globally. Betulinic acid (BA) is a pentacyclic triterpenoid with potential pro-apoptotic activities which widely found in many plants. In this study, we determined the effects of BA on proliferation, apoptosis, invasion, and metastasis in HCC cell lines and on tumour growth and pulmonary metastasis in mice. The results suggested that BA could inhibit cell viability and proliferation of HCC cell lines including HepG2, LM3, and MHCC97H...
November 12, 2018: Journal of Cellular and Molecular Medicine
Shanhu Qian, Yixiang Han, Yifen Shi, Wanling Xu, Yiyi Zhu, Songfu Jiang, Yi Chen, Zhijie Yu, Si Zhang, Yiping Yang, Kang Yu, Shenghui Zhang
Chronic exposure to benzene is known to be associated with haematotoxicity and the development of aplastic anaemia and leukaemia. However, the mechanism underlying benzene-induced haematotoxicity, especially at low concentrations of chronic benzene exposure has not been well-elucidated. Here, we found that increased autophagy and decreased acetylation occurred in bone marrow mononuclear cells (BMMNCs) isolated from patients with chronic benzene exposure. We further showed in vitro that benzene metabolite, hydroquinone (HQ) could directly induce autophagy without apoptosis in BMMNCs and CD34+ cells...
November 8, 2018: Journal of Cellular and Molecular Medicine
Mingjing Zhang, Pengfei Zhu, Yuting Wang, Jie Wu, Yijun Yu, Xinying Wu, Xiaoyan Liu, Ye Gu
Reducing sympathetic neurohormone expression is a key therapeutic option in attenuating cardiac remodelling. Present study tested the feasibility of attenuating cardiac remodelling through reducing sympathetic neurohormone level by partial cardiac sympathetic denervation in a rat model of chronic volume overload. Male Sprague-Dawley rats were randomized into sham group (S, n = 7), aortocaval fistula group (AV, n = 7), and aortocaval fistula with bilateral sympathetic stellate ganglionectomy group (AD, n = 8)...
November 8, 2018: Journal of Cellular and Molecular Medicine
Yibing Si, Junfeng Liu, Hongliang Shen, Chen Zhang, Yuanhao Wu, Yongyi Huang, Zhangbin Gong, Jun Xue, Te Liu
As a natural flavonol, fisetin has significant inhibitory effects on many cancers. Although fisetin can inhibit kidney cancer, its effects on kidney renal stem cells (HuRCSCs) remain unknown. Our study found that renal cancer tissues and CD44+/CD105+ HuRCSCs both show high TET1 protein expression. Both in vivo and in vitro experiments showed that fisetin can effectively inhibit HuRCSC cell division and proliferation, invasion, in vivo tumourigenesis and angiogenesis. Our findings showed that fisetin can significantly decrease TET1 expression levels in HuRCSCs and overall 5hmC levels in the genomes of these cells...
November 8, 2018: Journal of Cellular and Molecular Medicine
Guinever E Imperio, Mohsen Javam, Phetcharawan Lye, Andrea Constantinof, Caroline E Dunk, Fernando M Reis, Stephen J Lye, William Gibb, Stephen G Matthews, Tania Maria Ortiga-Carvalho, Enrrico Bloise
The ATP-binding cassette (ABC) transporters control placental transfer of several nutrients, steroids, immunological factors, chemicals, and drugs at the maternal-fetal interface. We and others have demonstrated a gestational age-dependent expression pattern of two ABC transporters, P-glycoprotein and breast cancer resistance protein throughout pregnancy. However, no reports have comprehensively elucidated the expression pattern of all 50 ABC proteins, comparing first trimester and term human placentae. We hypothesized that placental ABC transporters are expressed in a gestational-age dependent manner in normal human pregnancy...
November 8, 2018: Journal of Cellular and Molecular Medicine
Roser Guiteras, Anna Sola, Maria Flaquer, Anna Manonelles, Georgina Hotter, Josep M Cruzado
Alternatively activated macrophages (M2) have regenerative properties and shown promise as cell therapy in chronic kidney disease. However, M2 plasticity is one of the major hurdles to overcome. Our previous studies showed that genetically modified macrophages stabilized by neutrophil gelatinase-associated lipocalin (NGAL) were able to preserve their M2 phenotype. Nowadays, little is known about M2 macrophage effects in diabetic kidney disease (DKD). The aim of the study was to investigate the therapeutic effect of both bone marrow-derived M2 (BM-фM2) and ф-NGAL macrophages in the db/db mice...
November 8, 2018: Journal of Cellular and Molecular Medicine
Hitesh Soni, Anberitha T Matthews, Sandeep Pallikkuth, Rajashekhar Gangaraju, Adebowale Adebiyi
Organ toxicity, including kidney injury, limits the use of cisplatin for the treatment of multiple human cancers. Hence, interventions to alleviate cisplatin-induced nephropathy are of benefit to cancer patients. Recent studies have demonstrated that pharmacological inhibition of the Notch signaling pathway enhances cisplatin efficacy against several cancer cells. However, whether augmentation of the anti-cancer effect of cisplatin by Notch inhibition comes at the cost of increased kidney injury is unclear...
November 8, 2018: Journal of Cellular and Molecular Medicine
Xiaoming Liu, Jichun Liu, Xuekang Zhang, Yuben Tong, Xin Gan
Although the non-small cell lung cancer (NSCLC) is one of the most malignant tumours worldwide, the mechanisms controlling NSCLC tumourigenesis remain unclear. Here, we find that the expression of miR-520b is up-regulated in NSCLC samples. Further studies have revealed that miR-520b promotes the proliferation and metastasis of NSCLC cells. In addition, miR-520b activates Hedgehog (Hh) pathway. Inhibitor of Hh pathway could relieve the oncogenic effect of miR-520b upon NSCLC cells. Mechanistically, we demonstrate that miR-520b directly targets SPOP 3'-UTR and decreases SPOP expression, culminating in GLI2/3 stabilization and Hh pathway hyperactivation...
November 8, 2018: Journal of Cellular and Molecular Medicine
Wenguang Chang, Grant M Hatch, Yu Wang, Fei Yu, Man Wang
Insulin resistance induced by high-fat diet and impropriate life style is a major contributor to the pathogenesis of metabolic disease. However, the underlying molecular mechanisms remain unclear. Recent studies in metabolic dysfunction have extended this beyond simply elevated cholesterol and triglycerides levels and have identified a key role for lipid metabolism. For example, altered phospholipid metabolism has now become central in the pathogenesis of metabolic disease. In this review, we discuss the association between insulin sensitivity and phospholipid metabolism and highlight the most significant discoveries generated over the last several decades...
November 6, 2018: Journal of Cellular and Molecular Medicine
Ying Kong, Yan Gao, Dongyi Lan, Ying Zhang, Rixin Zhan, Meiqi Liu, Zhouan Zhu, Guohua Zeng, Qiren Huang
Previous study has shown that thiazolidinediones (TZDs) improved endothelium insulin resistance (IR) induced by high glucose concentration (HG)/hyperglycaemia through a PPARγ-dependent-NFκB trans-repression mechanism. However, it is unclear, whether changes in PPARγ expression affect the endothelium IR and what the underlying mechanism is. In the present study, we aimed to address this issue. HG-treated human umbilical vascular endothelial cells (HUVEC) were transfected by either PPARγ-overexpressing (Ad-PPARγ) or PPARγ-shRNA-containing (Ad-PPARγ-shRNA) adenoviral vectors...
November 5, 2018: Journal of Cellular and Molecular Medicine
Yuewang Song, Mengmeng Zhao, Yuan Xie, Tingfang Zhu, Wenbin Liang, Baiming Sun, Weixin Liu, Liqun Wu, Guoping Lu, Tao-Sheng Li, Tong Yin, Yucai Xie
Bmi-1 gene is well recognized as an oncogene, but has been recently demonstrated to play a role in the self-renewal of tissue-specific stem cells. By using Bmi-1GFP /+ mice, we investigated the role of Bmi-1 in cardiac stem/progenitor cells and myocardial repair. RT-PCR and flow cytometry analysis indicated that the expression of Bmi-1 was significantly higher in cardiac side population than the main population from CD45- Ter119- CD31- heart cells. More Sca-1+ cardiac stem/progenitor cells were found in Bmi-1 GFPhi subpopulation, and these Bmi-1 GFPhi heart cells showed the potential of differentiation into SMM+ smooth muscle-like cells and TnT+ cardiomyocyte-like cells in vitro...
November 5, 2018: Journal of Cellular and Molecular Medicine
Chao Wang, Chen Zhang, Xiang Li, Jiajia Shen, Yue Xu, Hui Shi, Xianmin Mu, Jinshun Pan, Ting Zhao, Mengjing Li, Biao Geng, Che Xu, Hao Wen, Qiang You
Gastric cancer (GC) is a malignancy of the lining of the stomach and is prone to distant metastasis, which involves a variety of complex molecules. The S100 proteins are a family of calcium-binding cytosolic proteins that possess a wide range of intracellular and extracellular functions and play pivotal roles in the invasion and migration of tumour cells. Among these, S100A10 is known to be overexpressed in GC. Lysine succinylation, a recently identified form of protein post-translational modification, is an important regulator of cellular processes...
November 5, 2018: Journal of Cellular and Molecular Medicine
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