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Experimental Biology and Medicine

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https://www.readbyqxmd.com/read/29444597/an-experimental-model-for-hypertensive-crises-emergencies-long-term-high-fat-diet-followed-by-acute-vasoconstriction-stress-on-spontaneously-hypertensive-rats
#1
Hong Liu, Wei-Wei Su, Chao-Feng Long, Wei-Jian Zhang, Pei-Bo Li, Zhong Wu, Yin-Yin Liao, Xuan Zeng, Tao-Bin Chen, Yu-Ying Zheng, Zeng-Hao Yan, Cong Bi, Hong-Liang Yao
Currently, the prevention and treatment of hypertensive crises especially when it occurs with serious adverse outcomes have led to worldwide controversy. Despite of clinical possibilities of multiple agents, clinical failures still occur frequently. Therefore, early evaluations and observations of different therapies on appropriate animals should be emphasized. In the present study, an animal model for hypertensive crises emergencies was firstly established and experimentally testified. Five-month-male spontaneously hypertensive rat was consecutively fed with 60%-Kcal fat diet for four, six, and eight weeks with body weight and blood pressure monitored every two weeks, and then followed by an acute vasoconstriction stress of 5-min ice-bath treatment in the 4-h time interval of two adrenaline injections (0...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29444596/activation-of-the-cannabinoid-receptor-1-by-acea-suppresses-senescence-in-human-primary-chondrocytes-through-sirt1-activation
#2
Dawei Zhang, Gang Zhang, Zongyu Li, Bingsheng Li
Senescence of chondrocytes and cartilage degeneration induced by the proinflammatory cytokine interleukin-1β is associated with the pathogenesis of osteoarthritis. The cannabinoid receptor 1 has been involved in the pathological development of various diseases. Here, we evaluated whether activation of cannabinoid receptor 1 using its selective agonist arachidonyl-2-chloroethylamide had an influence on cellular senescence induced by interleukin-1βin human chondrocytes. Our findings demonstrate that agonist arachidonyl-2-chloroethylamidedecreased senescence-associated β-galactosidase activity and cell cycle arrest in the G0/G1 phase induced by interleukin-1β...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29436846/effect-of-dimethyl-fumarate-on-renal-disease-progression-in-a-genetic-ortholog-of-nephronophthisis
#3
Oliver Oey, Padmashree Rao, Magdalena Luciuk, Carly Mannix, Natasha M Rogers, Priyanka Sagar, Annette Wong, Gopala Rangan
Dimethyl fumarate is an FDA-approved oral immunomodulatory drug with anti-inflammatory properties that induces the upregulation of the anti-oxidant transcription factor, nuclear factor erythroid-derived factor 2. The aim of this study was to determine the efficacy of dimethyl fumarate on interstitial inflammation and renal cyst growth in a preclinical model of nephronophthisis. Four-week-old female Lewis polycystic kidney disease (a genetic ortholog of human nephronophthisis-9) rats received vehicle (V), 10 mg/kg (D10) or 30 mg/kg (D30) ( n = 8-9 each) dimethyl fumarate in drinking water for eight weeks...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29415560/current-model-systems-for-the-study-of-preeclampsia
#4
M L Martinez-Fierro, G P Hernández-Delgadillo, V Flores-Morales, E Cardenas-Vargas, M Mercado-Reyes, I P Rodriguez-Sanchez, I Delgado-Enciso, C E Galván-Tejada, J I Galván-Tejada, J M Celaya-Padilla, I Garza-Veloz
Preeclampsia (PE) is a pregnancy complex disease, distinguished by high blood pressure and proteinuria, diagnosed after the 20th gestation week. Depending on the values of blood pressure, urine protein concentrations, symptomatology, and onset of disease there is a wide range of phenotypes, from mild forms developing predominantly at the end of pregnancy to severe forms developing in the early stage of pregnancy. In the worst cases severe forms of PE could lead to systemic endothelial dysfunction, eclampsia, and maternal and/or fetal death...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29412002/close-pathological-correlations-between-chronic-kidney-disease-and-reproductive-organ-associated-abnormalities-in-female-cotton-rats
#5
Osamu Ichii, Teppei Nakamura, Takao Irie, Hirokazu Kouguchi, Kozue Sotozaki, Taro Horino, Yuji Sunden, Yaser Hosny Ali Elewa, Yasuhiro Kon
Cotton rat ( Sigmodon hispidus) is a useful experimental rodent for the study of human infectious diseases. We previously clarified that cotton rats, particularly females, developed chronic kidney disease characterized by cystic lesions, inflammation, and fibrosis. The present study investigated female-associated factors for chronic kidney disease development in cotton rats. Notably, female cotton rats developed separation of the pelvic symphysis and hypertrophy in the vaginal parts of the cervix with age, which strongly associated with pyometra...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29409348/better-living-through-chemistry-addressing-emerging-antibiotic-resistance
#6
Nathan P Coussens, Ashley L Molinaro, Kayla J Culbertson, Tyler Peryea, Gergely Zahoránszky-Köhalmi, Matthew D Hall, Dayle A Daines
The increasing emergence of multidrug-resistant bacteria is recognized as a major threat to human health worldwide. While the use of small molecule antibiotics has enabled many modern medical advances, it has also facilitated the development of resistant organisms. This minireview provides an overview of current small molecule drugs approved by the US Food and Drug Administration (FDA) for use in humans, the unintended consequences of antibiotic use, and the mechanisms that underlie the development of drug resistance...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29409347/fryl-deficiency-is-associated-with-defective-kidney-development-and-function-in-mice
#7
Yong-Sub Byun, Eun-Kyoung Kim, Kimi Araki, Ken-Ichi Yamamura, Kihoon Lee, Won-Kee Yoon, Young-Suk Won, Hyoung-Chin Kim, Kyung-Chul Choi, Ki-Hoan Nam
FRY like transcription coactivator ( Fryl) gene located on chromosome 5 is a paralog of FRY microtubule binding protein ( Fry) in vertebrates. It encodes a protein with unknown functions. Fryl gene is conserved in various species ranging from eukaryotes to human. Although there are several reports on functions of Fry gene, functions of Fryl gene remain unclear. A mouse line containing null mutation in Fryl gene by gene trapping was produced in this study for the first time. The survival and growth of Fryl-/- mice were observed...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29402133/immunomodulatory-effect-of-hemozoin-on-pneumocyte-apoptosis-via-card9-pathway-a-possibly-retarding-pulmonary-resolution
#8
Sitang Maknitikul, Natthanej Luplertlop, Urai Chaisri, Yaowapa Maneerat, Sumate Ampawong
Plasmodium falciparum, the most virulent malaria parasite species, causes severe symptoms especially acute lung injury (ALI), of which characterized by alveolar epithelium and endothelium destruction and accelerated to blood-gas-barrier breakdown. Parasitized erythrocytes, endothelial cells, monocytes, and cytokines are all involved in this mechanism, but hemozoin (HZ), the parasitic waste from heme detoxification, also mainly contributes. In addition, it is not clear why type II pneumocyte proliferation, alveolar restorative stage, is rare in malaria-associated ALI...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29368542/mir-448-inhibits-the-epithelial-mesenchymal-transition-in-breast-cancer-cells-by-directly-targeting-the-e-cadherin-repressor-zeb1-2
#9
Peng Ma, Kan Ni, Jing Ke, Wenyi Zhang, Ying Feng, Qinsheng Mao
Recently, accumulating evidence provides that dysregulation of microRNAs (miRNAs) is considered to play vital roles in tumor progression. Based on microRNA arrays, we found that microRNA-448 (miR-448) was significantly downregulated in breast cancer tissue specimens. In our study, we were in an effort to clarify the function, the direct target gene, and the molecular mechanisms of miR-448 in breast cancer. By quantitative RT-PCR, we analyzed the expression of miR-448 in 16 patients with BC. Overexpression of miR-448 was established by transfecting miR-448-mimics into MDA-MB-231 and MCF-7 cells, methyl thiazolyl- tetrazolium and colony formation assays were performed to evaluate its effects on cell proliferation...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29363328/molecular-physiology-and-pathophysiology-of-stromal-interaction-molecules
#10
Heather A Nelson, Michael W Roe
Ca2+ release from the endoplasmic reticulum is an important component of Ca2+ signal transduction that controls numerous physiological processes in eukaryotic cells. Release of Ca2+ from the endoplasmic reticulum is coupled to the activation of store-operated Ca2+ entry into cells. Store-operated Ca2+ entry provides Ca2+ for replenishing depleted endoplasmic reticulum Ca2+ stores and a Ca2+ signal that regulates Ca2+-dependent intracellular biochemical events. Central to connecting discharge of endoplasmic reticulum Ca2+ stores following G protein-coupled receptor activation with the induction of store-operated Ca2+ entry are stromal interaction molecules (STIM1 and STIM2)...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29359577/phage-therapy-in-allergic-disorders
#11
Andrzej Górski, Ewa Jończyk-Matysiak, Marzanna Łusiak-Szelachowska, Ryszard Międzybrodzki, Beata Weber-Dąbrowska, Jan Borysowski
Allergic disorders pose a growing challenge to medicine and our society. Therefore, novel approaches to prevention and therapy are needed. Recent progress in studies on bacterial viruses (phages) has provided new data indicating that they have significant immunomodulating activities. We show how those activities could be translated into beneficial effects in allergic disorders and present initial clinical data that support this hope. Impact statement Allergic disorders pose a growing challenge to medicine and our society, so new approaches to prevention and therapy are urgently needed...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29350067/soluble-neuregulin1-is-strongly-up-regulated-in-the-rat-model-of-charcot-marie-tooth-1a-disease
#12
Benedetta Elena Fornasari, Giulia Ronchi, Davide Pascal, Davide Visigalli, Giovanna Capodivento, Lucilla Nobbio, Isabelle Perroteau, Angelo Schenone, Stefano Geuna, Giovanna Gambarotta
Neuregulin1 (NRG1) is a growth factor playing a pivotal role in peripheral nerve development through the activation of the transmembrane co-receptors ErbB2-ErbB3. Soluble NRG1 isoforms, mainly secreted by Schwann cells, are strongly and transiently up-regulated after acute peripheral nerve injury, thus suggesting that they play a crucial role also in the response to nerve damage. Here we show that in the rat experimental model of the peripheral demyelinating neuropathy Charcot-Marie-Tooth 1A (CMT1A) the expression of the different NRG1 isoforms (soluble, type α and β, type a and b) is strongly up-regulated, as well as the expression of NRG1 co-receptors ErbB2-ErbB3, thus showing that CMT1A nerves have a gene expression pattern highly reminiscent of injured nerves...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29350066/differential-expression-of-cytokeratin-14-and-18-in-bladder-cancer-tumorigenesis
#13
Yun-Peng Li, Xiao-Peng Jia, Yu-Qing Jiang, Wei Wang, Yun-Liang Wang, Xiu-Li Wang, Yue-Xian Guo
It has been previously suggested that cytokeratins (CKs) are important diagnostic and prognostic biomarkers for urothelial lesions. Hence it is imperative to understand the expression pattern of cytokeratins during formation of papillary bladder cancer, which was the objective of the current study. Expression pattern of CK14 and CK18 were examined using immunohistochemical staining in a mice model of papillary bladder cancer. Twenty female mice were divided into two groups-group 1 (NT) and group 2, which received N-butyl- N-(4-hydroxybutyl) nitrosamine (BBN) for 20 weeks plus one week without treatment...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29338309/oral-microbiota-reduce-wound-healing-capacity-of-epithelial-monolayers-irrespective-of-the-presence-of-5-fluorouracil
#14
Eline Vanlancker, Barbara Vanhoecke, Tom Sieprath, Janie Bourgeois, Annelore Beterams, Barbara De Moerloose, Winnok H De Vos, Tom Van de Wiele
Oral mucositis is still one of the most painful side effects of chemotherapeutic treatment and a mounting body of evidence suggests a key role for the oral microbiome in mucositis development. However, the underlying mechanisms remain elusive. In this work, we have investigated the interactions between the host, the microbiome, and chemotherapeutic treatments in more detail. The effect of 5-fluorouracil, commonly inducing mucositis, was assessed on a co-culture model that consists of an epithelial cell layer and a biofilm derived from oral microbiota from different types of samples (saliva, buccal swabs and tongue swabs) and donors (healthy individuals and patients suffering from mucositis)...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29316798/regulation-of-mitophagy-by-the-ubiquitin-pathway-in-neurodegenerative-diseases
#15
Shyamal Desai, Meredith Juncker, Catherine Kim
Mitophagy is a cellular process by which dysfunctional mitochondria are degraded via autophagy. Increasing empirical evidence proposes that this mitochondrial quality-control mechanism is defective in neurons of patients with various neurodegenerative diseases such as Ataxia Telangiectasia, Alzheimer's disease, Parkinson's disease, and Amyotrophic Lateral Sclerosis. Accumulation of defective mitochondria and the production of reactive oxygen species due to defective mitophagy have been identified as causes underlying neurodegenerative disease pathogenesis...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29310447/phosphorylation-de-phosphorylation-in-specific-sites-of-tumor-suppressor-wwox-and-control-of-distinct-biological-events
#16
Shenq-Shyang Huang, Nan-Shan Chang
Abnormal differentiation and growth of hematopoietic stem cells cause the development of hematopoietic diseases and hematopoietic malignancies. However, the molecular events underlying leukemia development are not well understood. In our recent study, we have demonstrated that calcium ionophore and phorbol ester force the differentiation of T lymphoblastic leukemia. The event involves a newly identified IκBα/WWOX/ERK signaling, in which WWOX is Ser14 phosphorylated. Additional evidence also reveals that pS14-WWOX is involved in enhancing cancer progression and metastasis and facilitating neurodegeneration...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29307283/modulation-of-the-ogf-ogfr-pathway-alters-cytokine-profiles-in-experimental-autoimmune-encephalomyelitis-and-multiple-sclerosis
#17
Michael D Ludwig, Ian S Zagon, Patricia J McLaughlin
The endogenous neuropeptide opioid growth factor, chemically termed [Met5]-enkephalin, has growth inhibitory and immunomodulatory properties. Opioid growth factor is distributed widely throughout most tissues, is autocrine and paracrine produced, and interacts at the nuclear-associated receptor, OGFr. Serum levels of opioid growth factor are decreased in patients with multiple sclerosis and in animals with experimental autoimmune encephalomyelitis suggesting that the OGF-OGFr pathway becomes dysregulated in this disease...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29307282/foxd3-foxd4-is-required-for-the-development-of-hindgut-in-the-rat-model-of-anorectal-malformation
#18
Luo-Jia Wang, Wei-Lin Wang, Hong Gao, Yu-Zuo Bai, Shu-Cheng Zhang
Congenital anorectal malformation is the most common digestive tract malformation in newborns. It has been reported that FOXD3/FOXD4, a forkhead transcription factor, regulates the generation, migration, and differentiation of neural crest cells. However, whether FOXD3/FOXD4 takes part in anorectal malformation remains unclear. In the present study, we used ethylene thiourea to induce the animal models of anorectal malformation in rat embryos and to interrogate the role of FOXD3/FOXD4 in anorectal malformation pathogenesis...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29307281/intermittent-fasting-protects-against-the-deterioration-of-cognitive-function-energy-metabolism-and-dyslipidemia-in-alzheimer-s-disease-induced-estrogen-deficient-rats
#19
Bae Kun Shin, Suna Kang, Da Sol Kim, Sunmin Park
Intermittent fasting may be an effective intervention to protect against age-related metabolic disturbances, although it is still controversial. Here, we investigated the effect of intermittent fasting on the deterioration of the metabolism and cognitive functions in rats with estrogen deficiency and its mechanism was also explored. Ovariectomized rats were infused with β-amyloid (25-35; Alzheimer's disease) or β-amyloid (35-25, Non-Alzheimer's disease; normal cognitive function) into the hippocampus. Each group was randomly divided into two sub-groups: one with intermittent fasting and the other fed ad libitum: Alzheimer's disease-ad libitum, Alzheimer's disease-intermittent fasting, Non-Alzheimer's disease-ad libitum, and Non-Alzheimer's disease-intermittent fasting...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29307280/myogenic-regulatory-factors-the-orchestrators-of-myogenesis-after-30-years-of-discovery
#20
Hasan A Asfour, Mohammed Z Allouh, Raed S Said
Prenatal and postnatal myogenesis share many cellular and molecular aspects. Myogenic regulatory factors are basic Helix-Loop-Helix transcription factors that indispensably regulate both processes. These factors (Myf5, MyoD, Myogenin, and MRF4) function as an orchestrating cascade, with some overlapped actions. Prenatally, myogenic regulatory factors are restrictedly expressed in somite-derived myogenic progenitor cells and their derived myoblasts. Postnatally, myogenic regulatory factors are important in regulating the myogenesis process via satellite cells...
January 1, 2018: Experimental Biology and Medicine
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