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Clinical and Experimental Medicine

Alireza Korourian, Raheleh Roudi, Ahmad Shariftabrizi, Elham Kalantari, Kambiz Sotoodeh, Zahra Madjd
Aberrant activation of Wnt and base excision repair (BER) signaling pathways are implicated in tumor progression and chemotherapy resistance in gastric adenocarcinoma. This study was conducted to clarify the role of E2F6 and RhoA, components of the Wnt signaling pathway, and SMUG1, a component of the BER pathway in gastric adenocarcinoma. Expression levels and clinicopathological significance of three biomarkers, namely E2F6, RhoA, and SMUG1, as potential signaling molecules involved in tumorigenesis and aggressive behavior, were examined using tissue microarray...
December 1, 2016: Clinical and Experimental Medicine
Mehrdad Asghari Estiar, Rezvan Esmaeili, Ali-Akbar Zare, Leila Farahmand, Hassan Fazilaty, Ali Zekri, Narges Jafarbeik-Iravani, Keivan Majidzadeh-A
Carcinoembryonic antigen (CEA) family members play important roles in malignancies and are introduced as biomarkers in different types of cancers. Among them CEACAM19 (CEAL1) gene, a new member of the CEA family, remains to be fully elucidated. The aim of this study was investigating the mRNA expression level of CEACAM19 in tumor samples of breast cancer patients compared to breast tissue of normal individuals. We evaluated the expression level of this gene in 75 breast tumors by using real-time quantitative PCR...
December 1, 2016: Clinical and Experimental Medicine
Hanna Romanowicz, Dominik Strapagiel, Marcin Słomka, Marta Sobalska-Kwapis, Ewa Kępka, Anna Siewierska-Górska, Marek Zadrożny, Jan Bieńkiewicz, Beata Smolarz
Breast cancer is the most common cause of malignancy and mortality in women worldwide. This study aimed at localising homologous recombination repair (HR) genes and their chromosomal loci and correlating their nucleotide variants with susceptibility to breast cancer. In this study, authors analysed the association between single nucleotide polymorphisms (SNPs) in homologous recombination repair genes and the incidence of breast cancer in the population of Polish women. Blood samples from 94 breast cancer patients were analysed as test group...
November 30, 2016: Clinical and Experimental Medicine
Shuai Lin, Meng Wang, Xinghan Liu, Wenge Zhu, Yan Guo, Zhiming Dai, Pengtao Yang, Tian Tian, Cong Dai, Yi Zheng, Chunyan Hu, Linyan Wei, Zhijun Dai
Macrophage migration inhibitory factor (MIF) has been reported to associate with increased cancer risk in several cancers. However, the role of MIF in breast cancer (BC) susceptibility remains unknown. For the first time, we conducted a case-control study to assess the potential association of three common MIF gene variants (rs755622, rs1803976, rs11548059) with BC susceptibility in Chinese women. Total 560 breast cancer patients and 583 age- and sex-matched healthy individuals were recruited from Northwest China, and the DNA was genotyped by Sequenom MassARRAY...
November 14, 2016: Clinical and Experimental Medicine
Tobias Ettl, Matthias Hautmann, Torsten E Reichert, Richard Bauer
The expression of the hair follicle stem cell marker CD34 was analyzed in five different head and neck squamous cell carcinoma (HNSCC) cell lines with different antibodies. All HNSCC cell lines expressed CD34 on their cell surface. After cell cycle synchronization via serum starvation, we observed cyclic CD34 expression in HNSCC cells dependent on cell cycle progression via immunofluorescent staining and flow cytometric analysis. Investigation of the CD34(+) and CD34(-) HNSCC populations revealed most of the cells in S-phase and G2/M-Phase in CD34(+) cells in contrast to CD34(-) cells...
November 11, 2016: Clinical and Experimental Medicine
Concetta Panebianco, Jude A Oben, Manlio Vinciguerra, Valerio Pazienza
Hepatic stellate cells (HSCs), also known as perisinusoidal cells, are pericytes found in the perisinusoidal space of the liver. HSCs are the major cell type involved in liver fibrosis, which is the formation of scar tissue in response to liver damage. When the liver is damaged, stellate cells can shift into an activated state, characterized by proliferation, contractility and chemotaxis. The activated HSCs secrete collagen scar tissue, which can lead to cirrhosis. Recent studies have shown that in vivo activation of HSCs by fibrogenic agents can eventually lead to senescence of these cells, which would contribute to reversal of fibrosis although it may also favor the insurgence of liver cancer...
September 21, 2016: Clinical and Experimental Medicine
Fernanda Buongusto, Claudio Bernardazzi, Agnes N Yoshimoto, Hayandra F Nanini, Raquel L Coutinho, Antonio Jose V Carneiro, Morgana T Castelo-Branco, Heitor S de Souza
Hedgehog (Hh) signaling is essential for intestinal homeostasis and has been associated with inflammation and tissue repair. We hypothesized that Hh signaling could affect the inflammatory process in inflammatory bowel disease (IBD). For this purpose, colon specimens from the inflamed and non-inflamed mucosa of 15 patients with Crohn's disease (CD), 15 with ulcerative colitis, and 15 controls were analyzed by immunohistochemistry and real-time PCR. The production and modulation of cytokines were measured by ELISA from culture explants...
September 21, 2016: Clinical and Experimental Medicine
Gabriele Valentini, Antonella Riccardi, Serena Vettori, Rosaria Irace, Michele Iudici, Salvatore Tolone, Ludovico Docimo, Marialuisa Bocchino, Alessandro Sanduzzi, Domenico Cozzolino
The aim of the study was to evaluate CXCL4 levels in undifferentiated connective tissue disease at risk for SSc (UCTD-SSc-risk) and confirm its increase and investigate its prognostic value. Serum CXCL4 levels were measured in 45 patients and 24 controls. CXCL4 was significantly higher in UCTD-SSc-risk patients than in controls. It resulted higher in patients with a shorter disease duration and in those lacking capillaroscopic alterations. We confirm that CXCL4 levels are increased in UCTD-risk-SSc patients...
September 20, 2016: Clinical and Experimental Medicine
Guglielmo Rd Villani, Giovanna Gallo, Emanuela Scolamiero, Francesco Salvatore, Margherita Ruoppolo
Organic acidurias are inherited metabolic diseases due to the deficiency of an enzyme or a transport protein involved in one of the several cellular metabolic pathways devoted to the catabolism of amino acids, carbohydrates or lipids. These deficiencies result in abnormal accumulation of organic acids in the body and their abnormal excretion in urine. More than 65 organic acidurias have been described; the incidence varies, individually, from 1 out of 10,000 to >1 out of 1000,000 live births. Collectively, their incidence approximates 1 out of 3000 live births...
September 9, 2016: Clinical and Experimental Medicine
Shan Meng, Chenyang Lu, Wanggang Zhang, Wenjun Shen, Yongchang Wei, Dan Su, Fuling Zhou
Multiple myeloma-associated antigen-1 (MMSA-1) is a novel multiple myeloma (MM)-associated antigen which has been recently identified. Herein, we have tried to examine its clinical significance by studying the relationship between its expression and selected clinicopathological features. We extracted mononuclear cells from the bone marrow of MM patients and healthy donors and compared the MMSA-1 expression by RT-PCR and Western blot analysis. In addition, we also analyzed MMSA-1 expression in patients that were grouped based on selected clinical parameters...
November 2016: Clinical and Experimental Medicine
Lucie Vavrova, Jana Rychlikova, Magdalena Mrackova, Olga Novakova, Ales Zak, Frantisek Novak
Markers of oxidative stress and antioxidant status in relation to inflammatory mediators in septic patients (SPs) during the course of sepsis and after recovery were analysed. Patients were 30 critically ill adults in severe sepsis/septic shock, 19 of which completed 3 samplings (S1: within 24 h after onset of sepsis, S7: 7 days after S1, R7: 7 days after clinical recovery). Comparing SPs with healthy controls (HCs), enhanced C-reactive protein, procalcitonin, bilirubin and CuZn-superoxide dismutase activity were found at S1 only...
November 2016: Clinical and Experimental Medicine
Dil Afroze, Adfar Yousuf, Nisar A Tramboo, Zaffar A Shah, Asrar Ahmad
Apolipoprotein E is a fundamental component of various lipoproteins and plays substantial role in cholesterol/lipid transport among cells of various tissues. The ApoE gene is polymorphic with three alleles ε2, ε3, and ε4, coding for isoforms E2, E3, and E4 having different binding inclination for corresponding receptors. This work aimed to investigate the association between ApoE gene polymorphism and coronary artery disease (CAD) in Kashmiri population. APOE genotyping was done by polymerase chain reaction-restriction fragment length polymorphism...
November 2016: Clinical and Experimental Medicine
Jan Novak, Jan Dobrovolny, Jitka Brozova, Lucie Novakova, Tomas Kozak
Immune reconstitution after high-dose chemotherapy and stem cell transplantation plays a key role in restoring immunocompetence including defense against infection, immune regulation, and onco-immune surveillance. In this work, we examined the recovery of mucosal-associated invariant T (MAIT) cells, recently discovered innate-like T cells, after various types of myeloablative chemotherapy and autologous peripheral blood stem cell transplantation in 29 patients. We show that MAIT cells are relatively resistant to myeloablative conditioning...
November 2016: Clinical and Experimental Medicine
L B L Maia, F S C Breginski, T C S Cavalcanti, R L R de Souza, V M S Roxo, E M S F Ribeiro
The Cyclin D1 protein has been extensively studied over the last decades, for its various roles in physiological processes, both in normal and cancer cells. Gene amplifications and overexpression of CCND1 are frequently reported in several types of cancers, including breast carcinomas, showing the increasing relevance of Cyclin D1 in tumorigenesis. Little is known about the role of this protein in the metastatic process, and the main objective of this study was to evaluate the importance of the CCND1 as a potential marker of tumor progression in breast carcinomas, in a sample collected in Southern Brazil...
November 2016: Clinical and Experimental Medicine
Anping Peng, Peifeng Ke, Rong Zhao, Xinyi Lu, Cheng Zhang, Xianzhang Huang, Guangjun Tian, Jun Huang, Jinli Wang, Pietro Invernizzi, Qubo Chen, Junhua Zhuang
Primary biliary cirrhosis (PBC) is a progressive autoimmune liver disease in which monocytes/macrophages infiltration and skewed T helper type (Th) 1 and Th17 cell responses participate in the development of the disease. Human peripheral blood monocytes are heterogeneous and can be divided into classical CD14(high)CD16(-), intermediate CD14(high)CD16(+), and nonclassical CD14(low)CD16(+) monocyte subsets. Compared to classical monocytes, CD16(+) monocytes are generally termed pro-inflammatory monocytes and play an important pathogenic role in autoimmune diseases...
November 2016: Clinical and Experimental Medicine
Joanna Rybka, S Mechiel Korte, Małgorzata Czajkowska-Malinowska, Małgorzata Wiese, Kornelia Kędziora-Kornatowska, Józef Kędziora
Depression is highly prevalent in COPD patients, and both diseases are believed to be associated with inflammation. The aim of this study was to elucidate the role of the immune system alterations in pathogenesis of depression in COPD patients. Blood was collected from patients diagnosed with chronic obstructive pulmonary disease and comorbid depressive symptoms [COPD + DS, (N = 13)], from individuals with either COPD (N = 16) or recurrent depressive disorder (rDD) alone (N = 15), and from healthy controls (N = 19)...
November 2016: Clinical and Experimental Medicine
Yoshinao Ohbatake, Sachio Fushida, Tomoya Tsukada, Jun Kinoshita, Katsunobu Oyama, Hironori Hayashi, Tomoharu Miyashita, Hidehiro Tajima, Hiroyuki Takamura, Itasu Ninomiya, Masakazu Yashiro, Kousei Hirakawa, Tetsuo Ohta
Paclitaxel (PTX) which easily elutes into ascites is widely used to treat gastric cancer patients with peritoneal carcinomatosis (PC), but clinical outcomes are suboptimal. Increased concentrations of α1-acid glycoprotein (AGP), an important drug-binding protein, have been reported in the plasma and ascites of cancer patients. This study sought to clarify whether AGP binds to PTX and alters its anticancer effects. AGP concentrations were measured in the serum and ascites of gastric cancer patients with PC and in the serum of healthy volunteers...
November 2016: Clinical and Experimental Medicine
Alexandra Papoudou-Bai, Eleftheria Hatzimichael, Alexandra Barbouti, Panagiotis Kanavaros
The activator protein-1 (AP-1) is a dimeric transcription factor composed of proteins belonging to the Jun (c-Jun, JunB and JunD), Fos (c-Fos, FosB, Fra1 and Fra2) and activating transcription factor protein families. AP-1 is involved in various cellular events including differentiation, proliferation, survival and apoptosis. Deregulated expression of AP-1 transcription factors is implicated in the pathogenesis of various lymphomas such as classical Hodgkin lymphomas, anaplastic large cell lymphomas, diffuse large B cell lymphomas and adult T cell leukemia/lymphoma...
September 6, 2016: Clinical and Experimental Medicine
Michelangelo Vestita, Lucia Lospalluti, Giuseppe Giudice, Domenico Bonamonte, Ignazio Rossiello, Angela Filoni
No abstract text is available yet for this article.
August 2, 2016: Clinical and Experimental Medicine
Franco Dammacco, Vito Racanelli, Sabino Russi, Domenico Sansonno
Cryoglobulinemic vasculitis (CV) is a small-to-medium-vessel vasculitis that appears in 10-15 % of patients chronically infected with hepatitis C virus (HCV). The classic symptom triad of CV, purpura/asthenia/arthralgia, is accompanied by clinical features that include glomerulonephritis, neuropathy, interstitial pneumonitis, and cardiomyopathy, ranging in their severity from mild to life threatening. The risk of developing non-Hodgkin lymphoma is also higher. The cumulative 10-year survival rate of CV patients is significantly lower than in the age- and sex-matched general population, with death typically caused by nephropathy, malignancies, liver involvement, and severe infections...
August 2016: Clinical and Experimental Medicine
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