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Tuberculosis

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https://www.readbyqxmd.com/read/29705064/corrigendum-to-a-whole-genome-bioinformatic-approach-to-determine-potential-latent-phase-specific-targets-in-mycobacterium-tuberculosis-tuberculosis-97-march-2016-181-192
#1
Lucas A Defelipe, Dario Fernandez Do Porto, Pablo Ivan Pereira Ramos, Marisa Fabiana Nicolas, Ezequiel Sosa, Leandro Radusky, Esteban Lanzarotti, Adrian G Turjanski, Marcelo A Marti
No abstract text is available yet for this article.
April 25, 2018: Tuberculosis
https://www.readbyqxmd.com/read/29615346/corrigendum-to-mechanisms-of-resistance-to-delamanid-a-drug-for-mycobacterium-tuberculosis-tuberculosis-108-january-2018-186-194
#2
Mamoru Fujiwara, Masanori Kawasaki, Norimitsu Hariguchi, Yongge Liu, Makoto Matsumoto
No abstract text is available yet for this article.
March 31, 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779781/tag-array-gene-chip-rapid-diagnosis-anti-tuberculosis-drug-resistance-in-pulmonary-tuberculosis-a-feasibility-study
#3
Wenjie Wu, Peng Cheng, Jingtong Lyu, Zehua Zhang, Jianzhong Xu
OBJECTIVE: We developed a Tag Array chip for detecting first- and second-line anti tuberculosis drug resistance in pulmonary tuberculosis and compared the analytical performance of the gene chip to that of phenotypic drug susceptibility testing (DST). METHODS: From November 2011 to April 2016.234 consecutive culture-confirmed, clinically and imaging diagnosed patients with pulmonary tuberculosis from Southwest Hospital, Chongqing were enrolled into the study. Specimens collected during sputum or bronchoalveolar lavage fluid from the pulmonary tuberculosis patients were subjected to M...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779780/on-sunspots-click-science-and-molecular-iconography
#4
Igor Mokrousov
CRISPR-spoligotyping and MIRU-VNTR typing, SITVIT_WEB and MIRU-VNTRplus are the methods and online resources most widely used for Mycobacterium tuberculosis genotype family assignment and clustering analysis. They have been proven invaluable for molecular epidemiological studies of this important human pathogen in setting up the terminology and classification framework. However, they are inherently limited by insufficient knowledge of evolution of the targeted genome loci (especially, CRISPR). The situation is aggravated by the dogmatic, iconographic perception of these increasingly user-friendly online tools...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779779/evaluation-of-pyrosequencing-for-extensive-drug-resistance-defining-anti-tuberculosis-drugs-for-use-in-public-healthcare
#5
Remya Nambiar, Daksha Shah, Kanchan Ajbani, Mubin Kazi, Meeta Sadani, Anjali Shetty, Padmaja Keskar, Sanjeev Kamble, Alex van Belkum, Camilla Rodrigues
MGIT 960 drug susceptibility testing (DST) for Mycobacterium tuberculosis was compared for performance and speed with pyrosequencing (PSQ). Pulmonary samples (n = 100), from GeneXpert/MTB/Rifampicin-resistant patients receiving second-line treatment for 1-3 months, were subjected to DST and PSQ for seven drugs (isoniazid, rifampicin, kanamycin, amikacin, capreomycin, moxifloxacin, and ofloxacin). The mean time-to-result was 35 and two days for DST and PSQ, respectively. Average concordancy was 92.7%. Theoretically, PSQ showed substantial incremental value over the commercial Genotype MTBDRplus/sl...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779778/identification-of-tb-e12-as-a-novel-ftsz-inhibitor-with-anti-tuberculosis-activity
#6
Yuan Lin, Hongjuan Zhang, Ningyu Zhu, Xia Wang, Yanxing Han, Minghua Chen, Jiandong Jiang, Shuyi Si
The global pandemic of multidrug-resistant (MDR) Mycobacterium tuberculosis (TB) drives for more effective anti-TB drugs with new drug target. Filamentous temperature sensitive protein Z (FtsZ), a GTP dependent prokaryotic cell division protein, forms a dynamic Z-ring in the center of the cell. Differences between bacterial FtsZ and eukaryotic tubulin make FtsZ a highly attractive drug target. In this study, we used phenotype screening of M. smegmatis and model screening targeting M. tuberculosis FtsZ (Mtb-FtsZ) to identify a hit compound TB-E12...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779777/interleukin-23-interleukin-17-axis-activated-by-mycobacterium-avium-complex-mac-is-attenuated-in-patients-with-mac-lung-disease
#7
Chin-Chung Shu, Jann-Yuan Wang, Ming-Fang Wu, Hsin-Chih Lai, Bor-Luen Chiang, Chong-Jen Yu
BACKGROUND: Mycobacterium avium complex (MAC)-lung disease (LD) is increasing in patients without human immunodeficiency virus infection. However, data on host vulnerability to MAC-related immune responses, and in particular the interleukin (IL)-23/IL-17 axis, are lacking. METHODS: We enrolled 50 patients with MAC-LD, 25 age-matched patients with tuberculosis (TB) and 25 controls. We measured levels of plasma cytokines, and studied IL-12/IL-17 responses in macrophage and lymphocyte activation to MAC...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779776/rd-rio-mycobacterium-tuberculosis-lineage-in-the-brazil-paraguay-argentina-triple-border
#8
Aryadne Larissa de Almeida, Regiane Bertin de Lima Scodro, Hayalla Corrêa de Carvalho, Giovana Ferreira Costacurta, Vanessa Pietrowski Baldin, Nathally Claudiane Souza Santos, Luciana Dias Ghiraldi-Lopes, Paula Aline Zanetti Campanerut-Sá, Vera Lucia Dias Siqueira, Katiany Rizzieri Caleffi-Ferracioli, Flávia Kazumi Shibata, Andressa Sprada, Rosilene Fressatti Cardoso
The high tuberculosis (TB) incidence rates, the closeness of the cities and the high migration flux on the Brazil/Paraguay/Argentina border deserves an in-depth study, using Mycobacterial Interspersed Repetitive Unit (MIRU) and Spoligotyping genetic markers to explore the impact of the Mycobacterium tuberculosis RDRio lineage on disease transmission and resistance to anti-TB drugs in this setting. Although without the totality of M. tuberculosis isolates causing TB in this studied setting, a number of 97 isolates obtained from sputa samples culture of patients with confirmed TB, from 2013 to 2015, were submitted to 24 loci MIRU, Spoligotyping, detection of RDRio lineage and detection of mutation related to isoniazid and rifampicin resistance by MTBDRplus/DNA STRIP...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779775/accuracy-of-whole-genome-sequencing-versus-phenotypic-mgit-and-commercial-molecular-tests-for-detection-of-drug-resistant-mycobacterium-tuberculosis-isolated-from-patients-in-brazil-and-mozambique
#9
Cinara Silva Feliciano, Evangelina Inacio Namburete, Jéssica Rodrigues Plaça, Kamila Peronni, Anzaan Dippenaar, Robin Mark Warren, Wilson Araújo Silva, Valdes Roberto Bollela
BACKGROUND: The fast and accurate diagnosis of drug-resistant tuberculosis (DR-TB) is critical to reducing the spread of disease. Although commercial genotypic drug-susceptibility tests (DST) are close to the goal, they are still not able to detect all relevant DR-TB related mutations. Whole genome sequencing (WGS) allows better comprehension of DR-TB with a great discriminatory power. We aimed to evaluate WGS in M. tuberculosis isolates compared with phenotypic and genotypic DST. METHODS: This cross-sectional study evaluated 30 isolates from patients with detected DR-TB in Brazil and Mozambique...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779774/the-impact-of-repeated-nalc-naoh-decontamination-on-the-performance-of-xpert-mtb-rif-assay
#10
Andrea Rachow, Elmar Saathoff, Bariki Mtafya, Daniel Mapamba, Chacha Mangu, Gabriel Rojas-Ponce, Nyanda E Ntinginya, Martin Boeree, Norbert Heinrich, Stephen H Gillespie, Michael Hoelscher
The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin ). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779773/reverse-line-probe-assay-for-cheap-detection-of-single-nucleotide-polymorphisms-in-mycobacterium-tuberculosis
#11
Memona Yasmin, Guislaine Refregier, Rubina Tabassum Siddiqui, Rizwan Iqbal, Shahid Ahmad Abbasi, Sabira Tahseen
More and more Single Nucleotide Polymosrphisms of interest among pathogenic organisms are described with the advent of Whole Genome Sequencing but WGS approach is still too expensive, time consuming, and relying on bioinformatical means that are not available in many developing countries. This study presents a low-cost reverse hybridization line probe technique for detecting SNPs in Mycobacterium tuberculosis. The proposed test is able to detect mutations in the RRDR of rpoB gene in M. tuberculosis with specificity and sensitivity of 98% and 100%, respectively and for an average cost of less than €3 per sample...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779772/dissecting-whole-genome-sequencing-based-online-tools-for-predicting-resistance-in-mycobacterium-tuberculosis-can-we-use-them-for-clinical-decision-guidance
#12
Rita Macedo, Alexandra Nunes, Isabel Portugal, Sílvia Duarte, Luís Vieira, João Paulo Gomes
Whole-genome sequencing (WGS)-based bioinformatics platforms for the rapid prediction of resistance will soon be implemented in the Tuberculosis (TB) laboratory, but their accuracy assessment still needs to be strengthened. Here, we fully-sequenced a total of 54 multidrug-resistant (MDR) and five susceptible TB strains and performed, for the first time, a simultaneous evaluation of the major four free online platforms (TB Profiler, PhyResSE, Mykrobe Predictor and TGS-TB). Overall, the sensitivity of resistance prediction ranged from 84...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779771/genetic-diversity-of-mycobacterium-tuberculosis-isoniazid-monoresistant-and-multidrug-resistant-in-rio-grande-do-sul-a-tuberculosis-high-burden-state-in-brazil
#13
Leonardo Souza Esteves, Elis Regina Dalla Costa, Sidra Ezidio Gonçalves Vasconcellos, Andrei Vargas, Sérgio Luis Montego Ferreira Junior, Maria Laura Halon, Marta Osorio Ribeiro, Rodrigo Rodenbusch, Harrison Magdinier Gomes, Philip N Suffys, Maria Lucia R Rossetti
Tuberculosis (TB) remains a major public health problem in the world and Brazil is among the countries with the highest incidence and prevalence rates, and Rio Grande do Sul, a Brazilian state, occupy a prominent position. Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) further aggravates this scenario, making it more difficult to treat and control the disease. Isoniazid monoresistance (IMR) may increase the risk of progression to MDR-TB and treatment failure. However, most drug resistance molecular tests only focus on detecting rifampicin (RIF) resistance...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779770/normalised-quantitative-polymerase-chain-reaction-for-diagnosis-of-tuberculosis-associated-uveitis
#14
Manas Ranjan Barik, Soveeta Rath, Rohit Modi, Rajkishori Rana, Mamatha M Reddy, Soumyava Basu
Polymerase chain reaction (PCR)-based diagnosis of tuberculosis-associated uveitis (TBU) in TB-endemic countries is challenging due to likelihood of latent mycobacterial infection in both immune and non-immune cells. In this study, we investigated normalised quantitative PCR (nqPCR) in ocular fluids (aqueous/vitreous) for diagnosis of TBU in a TB-endemic population. Mycobacterial copy numbers (mpb64 gene) were normalised to host genome copy numbers (RNAse P RNA component H1 [RPPH1] gene) in TBU (n = 16) and control (n = 13) samples (discovery cohort)...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779769/seasonal-variation-in-the-performance-of-quantiferon-tb-gold-in-tube-assays-used-for-the-diagnosis-of-tuberculosis-infection
#15
Marc Tebruegge, Nigel Curtis, Vanessa Clifford, Cristina Fernandez-Turienzo, Nigel Klein, Katy Fidler, Salah Mansour, Paul Elkington, Stephen Morris-Jones
This study aimed to determine whether there are seasonal changes in the performance of QuantiFERON-TB Gold In-Tube (QFT-GIT) assays, an interferon-gamma release assay widely used for the diagnosis of tuberculosis infection. Results of 31,932 QFT-GIT assays performed at a large independent, accredited diagnostic service provider in London, UK over a 4.5-year-period were analysed. The proportion of positive results was significantly lower in autumn (14.8%) than in spring (16.0%; p = 0.0366) and summer (17...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779768/genetic-diversity-of-antigen-38-kda-in-mycobacterium-tuberculosis-strains-from-china
#16
Yingzhen Du, Hongxia Li, Xuebing Qin, Yan Wang, Zhijian Zhang, Geping Qu, Xiangqun Fang, Senyang Yu
We used 335 Mycobacterium tuberculosis strains from 2010 National Epidemiologic Survey for TB in China and performed comparative sequence analysis of 38 kDa gene after amplification. From the results, we found that there were 5.07% M.tuberculosis strains that demonstrated genetic diversity of 38 kDa in China, and 2.99% strains showed polymorphism of the 38 kDa antigen, and this may be the reason for changes in the antigen produced, which may in turn cause alterations of related functions, thereby allowing immune evasion...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779767/novel-red-fluorescence-protein-based-microplate-assay-for-drug-screening-against-dormant-mycobacterium-tuberculosis-by-using-paraffin
#17
Amar Yeware, Dhiman Sarkar
The hypoxia model of dormancy is widely used in drug screening programs to identify novel inhibitors against latent Mycobacterium tuberculosis disease. In earlier reported microplate assays, hypoxia was maintained by either sealing the microplate or shifting in an anaerobic chamber to develop dormant phenotype. In these assays, inhibitors were added during inoculation, which mainly represents the active stage inhibitors instead of the dormant ones. Herein, the culture was covered with paraffin to develop hypoxia condition and consequently providing the advantage of adding compounds at any stage during incubation of 96-well plate...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779766/should-all-suspected-tuberculosis-cases-in-high-income-countries-be-tested-with-genexpert
#18
Venanzio Vella, Agnieszka Broda, Francis Drobniewski
In countries with a low incidence of multidrug-resistant tuberculosis (MDR-TB), universal testing with GeneXpert might not be always cost-effective. This study provides hospital managers in low MDR-TB incidence countries with criteria on when decentralised universal GeneXpert testing would make sense. The alternatives taken into consideration include: universal microbiological culture and drug susceptibility testing (DST) only (comparator); as above but with concurrent centralized GeneXpert in a referral laboratory vs a decentralized GeneXpert system in every hospital to test smear-positive cases only; as above but testing all samples with GeneXpert regardless of smear status...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779765/prolonged-intervals-during-mycobacterium-tuberculosis-subunit-vaccine-boosting-contributes-to-eliciting-immunity-mediated-by-central-memory-like-t-cells
#19
Chunxiang Bai, Juanjuan He, Hongxia Niu, Lina Hu, Yanping Luo, Xun Liu, Liang Peng, Bingdong Zhu
It is believed that central memory T cells (TCM ) provide long-term protection against tuberculosis (TB). However, the effects of TB subunit vaccine immunization schedule, especially the vaccination intervals, on T cell immune memory is still unclear. In this study, mice were immunized with fusion protein ESAT6-Ag85B-MPT64 (190-198)-Mtb8.4-Rv2626c (LT70) based subunit vaccine three times according to the following schedules: ① 0, 3rd and 6th week respectively (0-3-6w), ② 0, 4th and 12th week (0-4-12w), and ③ 0, 4th and 24th week (0-4-24w)...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779764/experimental-animal-models-of-central-nervous-system-tuberculosis-a-historical-review
#20
REVIEW
Carlos Sánchez-Garibay, María Elena Hernández-Campos, Martha Lilia Tena-Suck, Citlaltepetl Salinas-Lara
Animal models are and will remain valuable tools in medical research because their use enables a deeper understanding of disease development, thus generating important knowledge for developing disease control strategies. Central nervous system tuberculosis (CNS TB) is the most devastating disease in humans. Moreover, as the variability of signs and symptoms delay a timely diagnosis, patients usually arrive at the hospital suffering from late stage disease. Therefore, it is impossible to obtain fresh human tissue for research before an autopsy...
May 2018: Tuberculosis
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